Rh7CG400000

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0008218
Physical Location & Seq
Forward (+)
51117008 .. 51140325
23318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG400000.1

Sequence Viewer

Length: 2058 bp
ATGAGTGGAAGAGCTAGCAGATGTGTACCTAAATCCATGTTTGAGCGGTTCACAGACTTTTTGCCTTGGGGTTCCGAAAGTCAAAATGGGAATTCAAGTGAAAGAGAGGATGATGAAACTAAAATTCAAAATGGGGATACAAGTGGAAGAGGGCATGATGAGACTAAAACGCCAACTCTAGAGGAGTGTGGGACTAATTTAACGAAGCTAGCAGAAGAGGGCAAGCTGGATCCTGTTTTTGGAAGAGAAGAACAAATATCACGTGTCATCCAAATTTTGAGTAGGCGGACTAAGAATAATGCCTGCATTATTGGAGAACCAAGTGTTGGGAAGACTGCAATTGCAGAAGGTCTTGCTCAACGTATTGCAAGTGGCAATGTTCCAAATTCAATAGCCAACAAGAAGGTTATAAGACTTGATATGGCTAAACTTGTCTCTTGTACCAAGTACCGTGGAGAGTTTGAGGGAAAATTGAAGATGCTAATGGAGGAAATAGAAAAAAGTGATGGTCAAGTTATTTTGTTTATCGATGAGATGCACAATTTAATGGGAGCAGGAGCAGCAGCAGGAGGAATTGATGCTTCAAATTTATTGAAGCCGGCTCTTGCAAGAGCCAAACAGATTTTGAGAGGGCTTCGAGAACGATATGAGATCCACCACAAAGTCCAGTATACTGATGAGGCCCTGAAAGCAGCTGCACAGCTATCTCACCGATACATCAGCGATCGCTTTCTTCCTGATAAAGCAATTGACTTGATCGATGAAGCTGGTTCTGCAGTAAGGCTGCGTCCTGCACAGGTTCGTAAAGAAGCTCAAGAGCTTGACAATATTCTGAGGCAGATAACAAAAGAGAAGAATGAAGCTATTCGTAGCCATGAGTTTGAGAAGGCTGCGCAATTAGGTGACAGAGAATTGGACCTTAAGACACAAATCTCAGAAGTAATAGAGAAAGGCAGGGAGATAAGTGAGGCATTGGATTATGTTAATGGTGGCCGTATTGTTATTGAATTGGATATTCAACAAGTTGTGTCCTGTATGACTAGTATTCCACTTGAGGAAGTGTCAACAAATGAAGCCAATCGCCTCCTCAAGATGGAAGAGACCCTCCATAAGCGGGTTATTGGTCAGGATGAAGCAGTCAGCGCTATTAGCAGAGCTATTCGTCGAGCTCGTGTTGGACTGAGGAATCCTGATCGGCCAATTGCCAGTTTCATCTTTTGTGGTCCAACAGGTGTTGGAAAATCTGAGCTCGTAAAAACTTTGGCGACTAGCTATTTTGGCTCTGAAGAATCAATGGTAAGGCTCGATATGAGTGAGTTCATGGAAGAGCACTCTGTTTCCAAGCGCATTGGTTCTCCCCCTGGTTATGTTGGTTACACAGAAGGCGGTCAGCTGGTTGAAGCCGTTTGTCGCCGTCCTTACACTCTGGTGCTTTTTGATGAAATTAAGAAGGCTCATCCTAAAATTTTCAACTTGATGCTTCAAATACTTGAGGATGGAAGATTGACTGACTGCAAAGGCAGAACTATAGATTTCAAGAATACACTAGTCATTATGACGTCTAATGTTGGAAGCAGTGAGATTGAGAGAGGAGGCCGCAAAATGGGATTTGACCTGGCCATGGATGAAGACAAGGATGGCAGTTACAAACAAATTAAGGGCTTGGTGACAGAGAAATTGAAACACCACTTTCGACCAGAGTTCTTAAATAGGTTGGATGAAATCATTGTTTTTCGGCAGCTGACTAAGTTGGACGTAAAGGAAATTGCAGATATAATGCTGAACGAGTATTATGGAATGCTCAAGACCAAAGAGATAGAGCTTAGGGTGACAAAGAGGTTCAAAGACAAATTGGTTGACGAAGGATACAACCCAACCTATGGAGCAAGACCTTTAAGACCTTTAATGAGACTTTTGGTGGACACCATGGCTGACAAGATGCTGGCAAAGGAGATCAAAGAGGGCGACTCAGTTATTCTAGATGTCGATTCTGATGGGAATGTAATTATGTTCATCAATGGCAGCACCAAGATAGAGATTAAAGAAATAGGCGATTGA

Protein Analysis

685

Amino Acids

76.86

Weight (kDa)

6.05

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NBD_SMAX1 PF23569 83 - 177 5.2e-07 SMAX1 nucleotide binding domain
AAA PF00004 102 - 199 7.1e-11 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 204 - 301 1.5e-33 AAA lid domain
AAA_2 PF07724 399 - 575 2.3e-62 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 404 - 525 4e-10 AAA domain (dynein-related subfamily)
ClpB_D2-small PF10431 581 - 659 1.8e-18 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 410
AatII GACGTC 1 cut(s) 1562
Acc16I TGCGCA 1 cut(s) 894
AccB7I CCANNNNNTGG 2 cut(s) 326, 1880
AccBSI CCGCTC 1 cut(s) 46
AccI GTMKAC 1 cut(s) 671
AciI CCGC 5 cut(s) 46, 286, 1114, 1386, 1597
AclWI GGATC 3 cut(s) 224, 237, 646
AcoI YGGCCR 3 cut(s) 991, 1196, 1617
AcsI RAATTY 6 cut(s) 91, 123, 273, 385, 586, 1464
AcuI CTGAAG 1 cut(s) 1305
AcvI CACGTG 1 cut(s) 263
AcyI GRCGYC 1 cut(s) 1559
AfaI GTAC 3 cut(s) 27, 442, 449
AfeI AGCGCT 1 cut(s) 1144
AfiI CCNNNNNNNGG 7 cut(s) 239, 326, 1093, 1114, 1603, 1621, 1880
AflII CTTAAG 1 cut(s) 920
AflIII ACRYGT 1 cut(s) 262
AhlI ACTAGT 2 cut(s) 1040, 1546
AjnI CCWGG 2 cut(s) 1360, 1614
AjuI GAANNNNNNNTTGG 2 cut(s) 309, 341
AleI CACNNNNGTG 1 cut(s) 1427
Alw21I GWGCWC 3 cut(s) 1171, 1251, 1332
Alw26I GTCTC 4 cut(s) 155, 439, 1094, 1903
AlwI GGATC 3 cut(s) 224, 237, 646
Aor51HI AGCGCT 1 cut(s) 1144
AoxI GGCC 5 cut(s) 681, 991, 1196, 1594, 1617
ApeKI GCWGC 8 cut(s) 560, 563, 692, 695, 784, 890, 1738, 2022
ApoI RAATTY 6 cut(s) 91, 123, 273, 385, 586, 1464
AsiSI GCGATCGC 1 cut(s) 727
Asp700I GAANNNNTTC 1 cut(s) 864
AspLEI GCGC 3 cut(s) 895, 1145, 1347
AspS9I GGNCC 3 cut(s) 682, 916, 1223
AsuHPI GGTGA 4 cut(s) 701, 914, 1678, 1840
AsuNHI GCTAGC 2 cut(s) 14, 208
AvaII GGWCC 2 cut(s) 916, 1223
BalI TGGCCA 1 cut(s) 1619
BamHI GGATCC 1 cut(s) 229
BanII GRGCYC 2 cut(s) 1171, 1251
BauI CACGAG 1 cut(s) 1170
BbrPI CACGTG 1 cut(s) 263
BbsI GAAGAC 2 cut(s) 338, 1635
Bbv12I GWGCWC 3 cut(s) 1171, 1251, 1332
BbvI GCAGC 8 cut(s) 572, 575, 682, 704, 771, 877, 1750, 2034
BccI CCATC 5 cut(s) 500, 1087, 1490, 1631, 1988
BceAI ACGGC 3 cut(s) 978, 1388, 1398
BciT130I CCWGG 2 cut(s) 1362, 1616
BciVI GTATCC 2 cut(s) 130, 1859
BcoDI GTCTC 4 cut(s) 155, 439, 1094, 1903
BcuI ACTAGT 2 cut(s) 1040, 1546
BfaI CTAG 7 cut(s) 15, 179, 209, 1041, 1269, 1547, 1979
BfmI CTRYAG 2 cut(s) 774, 1527
BfoI RGCGCY 1 cut(s) 1146
BfrI CTTAAG 1 cut(s) 920
BfuI GTATCC 2 cut(s) 130, 1859
BisI GCNGC 9 cut(s) 561, 564, 693, 696, 785, 891, 1597, 1739, 2023
BlsI GCNGC 9 cut(s) 562, 565, 694, 697, 786, 892, 1598, 1740, 2024
Bme1390I CCNGG 2 cut(s) 1362, 1616
Bme18I GGWCC 2 cut(s) 916, 1223
BmgT120I GGNCC 3 cut(s) 682, 916, 1223
BmiI GGNNCC 2 cut(s) 73, 231
BmrFI CCNGG 2 cut(s) 1362, 1616
BmsI GCATC 5 cut(s) 468, 525, 568, 1467, 1929
BmtI GCTAGC 2 cut(s) 18, 212
BpiI GAAGAC 2 cut(s) 338, 1635
BplI GAGNNNNNCTC 2 cut(s) 1952, 1984
Bpu10I CCTNAGC 1 cut(s) 1823
BpuEI CTTGAG 5 cut(s) 798, 1073, 1073, 1511, 1787
Bsa29I ATCGAT 2 cut(s) 528, 759
BsaAI YACGTR 1 cut(s) 263
BsaHI GRCGYC 1 cut(s) 1559
BsaI GGTCTC 1 cut(s) 1094
BsaJI CCNNGG 5 cut(s) 65, 451, 1360, 1620, 1926
Bsc4I CCNNNNNNNGG 7 cut(s) 239, 326, 1093, 1114, 1603, 1621, 1880
Bse118I RCCGGY 1 cut(s) 598
Bse1I ACTGG 2 cut(s) 667, 1206
Bse3DI GCAATG 1 cut(s) 382
BseBI CCWGG 2 cut(s) 1362, 1616
BseCI ATCGAT 2 cut(s) 528, 759
BseDI CCNNGG 5 cut(s) 65, 451, 1360, 1620, 1926
BseGI GGATG 8 cut(s) 115, 267, 1135, 1456, 1501, 1630, 1642, 1723
BseLI CCNNNNNNNGG 7 cut(s) 239, 326, 1093, 1114, 1603, 1621, 1880
BseMI GCAATG 1 cut(s) 382
BseMII CTCAG 5 cut(s) 824, 948, 1172, 1236, 1983
BseNI ACTGG 2 cut(s) 667, 1206
BseRI GAGGAG 3 cut(s) 197, 1076, 1605
BseXI GCAGC 8 cut(s) 572, 575, 682, 704, 771, 877, 1750, 2034
BsgI GTGCAG 2 cut(s) 681, 777
Bsh1285I CGRYCG 1 cut(s) 727
BshFI GGCC 5 cut(s) 683, 993, 1198, 1596, 1619
BshVI ATCGAT 2 cut(s) 528, 759
BsiEI CGRYCG 1 cut(s) 727
BsiHKAI GWGCWC 3 cut(s) 1171, 1251, 1332
BsiSI CCGG 1 cut(s) 599
BslFI GGGAC 1 cut(s) 205
BslI CCNNNNNNNGG 7 cut(s) 239, 326, 1093, 1114, 1603, 1621, 1880
BsmAI GTCTC 4 cut(s) 155, 439, 1094, 1903
BsmFI GGGAC 1 cut(s) 205
BsmI GAATGC 1 cut(s) 1803
BsnI GGCC 5 cut(s) 683, 993, 1198, 1596, 1619
Bso31I GGTCTC 1 cut(s) 1094
Bsp1286I GDGCHC 3 cut(s) 1171, 1251, 1332
Bsp143I GATC 6 cut(s) 229, 651, 724, 756, 1192, 1953
Bsp19I CCATGG 2 cut(s) 1620, 1926
BspACI CCGC 5 cut(s) 46, 286, 1114, 1386, 1597
BspANI GGCC 5 cut(s) 683, 993, 1198, 1596, 1619
BspCNI CTCAG 5 cut(s) 825, 947, 1173, 1237, 1982
BspDI ATCGAT 2 cut(s) 528, 759
BspLI GGNNCC 2 cut(s) 73, 231
BspMAI CTGCAG 1 cut(s) 778
BspOI GCTAGC 2 cut(s) 18, 212
BspPI GGATC 3 cut(s) 224, 237, 646
BspQI GCTCTTC 2 cut(s) 4, 1320
BspTI CTTAAG 1 cut(s) 920
BspTNI GGTCTC 1 cut(s) 1094
BsrBI CCGCTC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 382
BsrFI RCCGGY 1 cut(s) 598
BsrI ACTGG 2 cut(s) 667, 1206
BssAI RCCGGY 1 cut(s) 598
BssECI CCNNGG 5 cut(s) 65, 451, 1360, 1620, 1926
BssMI GATC 6 cut(s) 229, 651, 724, 756, 1192, 1953
BssNAI GTATAC 1 cut(s) 672
BssNI GRCGYC 1 cut(s) 1559
BssSI CACGAG 1 cut(s) 1170
BssT1I CCWWGG 3 cut(s) 65, 1620, 1926
Bst1107I GTATAC 1 cut(s) 672
Bst2BI CACGAG 1 cut(s) 1170
Bst2UI CCWGG 2 cut(s) 1362, 1616
Bst4CI ACNGT 1 cut(s) 452
Bst6I CTCTTC 6 cut(s) 4, 142, 210, 238, 1092, 1320
BstACI GRCGYC 1 cut(s) 1559
BstAFI CTTAAG 1 cut(s) 920
BstBAI YACGTR 1 cut(s) 263
BstC8I GCNNGC 6 cut(s) 16, 210, 224, 304, 600, 1944
BstDEI CTNAG 8 cut(s) 291, 833, 934, 1181, 1245, 1746, 1823, 1969
BstDSI CCRYGG 3 cut(s) 451, 1620, 1926
BstF5I GGATG 8 cut(s) 115, 267, 1135, 1456, 1501, 1630, 1642, 1723
BstH2I RGCGCY 1 cut(s) 1146
BstHHI GCGC 3 cut(s) 895, 1145, 1347
BstKTI GATC 6 cut(s) 232, 654, 727, 759, 1195, 1956
BstMAI GTCTC 4 cut(s) 155, 439, 1094, 1903
BstMBI GATC 6 cut(s) 229, 651, 724, 756, 1192, 1953
BstMCI CGRYCG 1 cut(s) 727
BstMWI GCNNNNNNNGC 6 cut(s) 560, 689, 773, 1142, 1149, 1278
BstNI CCWGG 2 cut(s) 1362, 1616
BstSCI CCNGG 2 cut(s) 1360, 1614
BstSFI CTRYAG 2 cut(s) 774, 1527
BstV1I GCAGC 8 cut(s) 572, 575, 682, 704, 771, 877, 1750, 2034
BstV2I GAAGAC 2 cut(s) 338, 1635
BstX2I RGATCY 2 cut(s) 229, 651
BstYI RGATCY 2 cut(s) 229, 651
BstZ17I GTATAC 1 cut(s) 672
Bsu15I ATCGAT 2 cut(s) 528, 759
BsuI GTATCC 2 cut(s) 130, 1859
BsuRI GGCC 5 cut(s) 683, 993, 1198, 1596, 1619
BsuTUI ATCGAT 2 cut(s) 528, 759
BtgI CCRYGG 3 cut(s) 451, 1620, 1926
BtsCI GGATG 8 cut(s) 115, 267, 1135, 1456, 1501, 1630, 1642, 1723
BtsI GCAGTG 1 cut(s) 1582
BtsIMutI CAGTG 1 cut(s) 1582
Cac8I GCNNGC 6 cut(s) 16, 210, 224, 304, 600, 1944
CfoI GCGC 3 cut(s) 895, 1145, 1347
Cfr10I RCCGGY 1 cut(s) 598
Cfr13I GGNCC 3 cut(s) 682, 916, 1223
ClaI ATCGAT 2 cut(s) 528, 759
CseI GACGC 1 cut(s) 776
Csp6I GTAC 3 cut(s) 26, 441, 448
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviAII CATG 6 cut(s) 37, 155, 875, 1321, 1621, 1927
CviQI GTAC 3 cut(s) 26, 441, 448
DdeI CTNAG 8 cut(s) 291, 833, 934, 1181, 1245, 1746, 1823, 1969
DpnI GATC 6 cut(s) 231, 653, 726, 758, 1194, 1955
DpnII GATC 6 cut(s) 229, 651, 724, 756, 1192, 1953
EaeI YGGCCR 3 cut(s) 991, 1196, 1617
Eam1104I CTCTTC 6 cut(s) 4, 142, 210, 238, 1092, 1320
EarI CTCTTC 6 cut(s) 4, 142, 210, 238, 1092, 1320
EciI GGCGGA 1 cut(s) 301
Ecl136II GAGCTC 2 cut(s) 1169, 1249
Eco130I CCWWGG 3 cut(s) 65, 1620, 1926
Eco24I GRGCYC 2 cut(s) 1171, 1251
Eco31I GGTCTC 1 cut(s) 1094
Eco47I GGWCC 2 cut(s) 916, 1223
Eco47III AGCGCT 1 cut(s) 1144
Eco53kI GAGCTC 2 cut(s) 1169, 1249
Eco57I CTGAAG 1 cut(s) 1305
Eco72I CACGTG 1 cut(s) 263
EcoICRI GAGCTC 2 cut(s) 1169, 1249
EcoO109I RGGNCCY 1 cut(s) 682
EcoRI GAATTC 1 cut(s) 91
EcoRII CCWGG 2 cut(s) 1360, 1614
EcoT14I CCWWGG 3 cut(s) 65, 1620, 1926
EcoT38I GRGCYC 2 cut(s) 1171, 1251
ErhI CCWWGG 3 cut(s) 65, 1620, 1926
FaeI CATG 6 cut(s) 40, 158, 878, 1324, 1624, 1930
FaqI GGGAC 1 cut(s) 205
FatI CATG 6 cut(s) 36, 154, 874, 1320, 1620, 1926
FauI CCCGC 1 cut(s) 1107
FblI GTMKAC 1 cut(s) 671
Fnu4HI GCNGC 9 cut(s) 561, 564, 693, 696, 785, 891, 1597, 1739, 2023
FokI GGATG 8 cut(s) 122, 254, 1142, 1443, 1508, 1637, 1649, 1730
FriOI GRGCYC 2 cut(s) 1171, 1251
Fsp4HI GCNGC 9 cut(s) 561, 564, 693, 696, 785, 891, 1597, 1739, 2023
FspBI CTAG 7 cut(s) 15, 179, 209, 1041, 1269, 1547, 1979
FspI TGCGCA 1 cut(s) 894
GlaI GCGC 3 cut(s) 894, 1144, 1346
GluI GCNGC 9 cut(s) 561, 564, 693, 696, 785, 891, 1597, 1739, 2023
HaeII RGCGCY 1 cut(s) 1146
HaeIII GGCC 5 cut(s) 683, 993, 1198, 1596, 1619
HapII CCGG 1 cut(s) 599
HgaI GACGC 1 cut(s) 776
HhaI GCGC 3 cut(s) 895, 1145, 1347
Hin1I GRCGYC 1 cut(s) 1559
Hin1II CATG 6 cut(s) 40, 158, 878, 1324, 1624, 1930
Hin6I GCGC 3 cut(s) 893, 1143, 1345
HinP1I GCGC 3 cut(s) 893, 1143, 1345
HincII GTYRAC 2 cut(s) 1065, 1858
HindII GTYRAC 2 cut(s) 1065, 1858
HinfI GANTC 4 cut(s) 1186, 1289, 1967, 1988
HpaII CCGG 1 cut(s) 599
HphI GGTGA 4 cut(s) 701, 914, 1678, 1840
Hpy166II GTNNAC 6 cut(s) 26, 51, 672, 1065, 1858, 1921
Hpy188I TCNGA 6 cut(s) 76, 834, 937, 1246, 1285, 1993
Hpy8I GTNNAC 6 cut(s) 26, 51, 672, 1065, 1858, 1921
Hpy99I CGWCG 1 cut(s) 1167
HpyAV CCTTC 6 cut(s) 341, 397, 880, 1376, 1444, 1856
HpyCH4III ACNGT 1 cut(s) 452
HpyCH4IV ACGT 4 cut(s) 262, 361, 1559, 1755
HpyF10VI GCNNNNNNNGC 6 cut(s) 560, 689, 773, 1142, 1149, 1278
HpyF3I CTNAG 8 cut(s) 291, 833, 934, 1181, 1245, 1746, 1823, 1969
HpySE526I ACGT 4 cut(s) 262, 361, 1559, 1755
Hsp92I GRCGYC 1 cut(s) 1559
Hsp92II CATG 6 cut(s) 40, 158, 878, 1324, 1624, 1930
HspAI GCGC 3 cut(s) 893, 1143, 1345
KroI GCCGGC 1 cut(s) 598
KroNI GCCGGC 1 cut(s) 600
Kzo9I GATC 6 cut(s) 229, 651, 724, 756, 1192, 1953
LguI GCTCTTC 2 cut(s) 4, 1320
LmnI GCTCC 3 cut(s) 551, 557, 1883
Lsp1109I GCAGC 8 cut(s) 572, 575, 682, 704, 771, 877, 1750, 2034
LweI GCATC 5 cut(s) 468, 525, 568, 1467, 1929
MaeI CTAG 7 cut(s) 15, 179, 209, 1041, 1269, 1547, 1979
MaeII ACGT 4 cut(s) 262, 361, 1559, 1755
MaeIII GTNAC 5 cut(s) 902, 1373, 1643, 1666, 1828
MalI GATC 6 cut(s) 231, 653, 726, 758, 1194, 1955
MbiI CCGCTC 1 cut(s) 46
MboI GATC 6 cut(s) 229, 651, 724, 756, 1192, 1953
MfeI CAATTG 3 cut(s) 339, 747, 1200
MflI RGATCY 2 cut(s) 229, 651
MhlI GDGCHC 3 cut(s) 1171, 1251, 1332
MlsI TGGCCA 1 cut(s) 1619
MluNI TGGCCA 1 cut(s) 1619
MlyI GAGTC 1 cut(s) 1961
MmeI TCCRAC 6 cut(s) 1156, 1216, 1250, 1549, 1695, 1731
Mox20I TGGCCA 1 cut(s) 1619
MroNI GCCGGC 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 864
MscI TGGCCA 1 cut(s) 1619
MslI CAYNNNNRTG 1 cut(s) 1427
Msp20I TGGCCA 1 cut(s) 1619
MspA1I CMGCKG 3 cut(s) 695, 1393, 1741
MspCI CTTAAG 1 cut(s) 920
MspI CCGG 1 cut(s) 599
MspR9I CCNGG 2 cut(s) 1362, 1616
MunI CAATTG 3 cut(s) 339, 747, 1200
Mva1269I GAATGC 1 cut(s) 1803
MvaI CCWGG 2 cut(s) 1362, 1616
MwoI GCNNNNNNNGC 6 cut(s) 560, 689, 773, 1142, 1149, 1278
NaeI GCCGGC 1 cut(s) 600
NcoI CCATGG 2 cut(s) 1620, 1926
NdeII GATC 6 cut(s) 229, 651, 724, 756, 1192, 1953
NgoMIV GCCGGC 1 cut(s) 598
NheI GCTAGC 2 cut(s) 14, 208
NlaIII CATG 6 cut(s) 40, 158, 878, 1324, 1624, 1930
NlaIV GGNNCC 2 cut(s) 73, 231
NmuCI GTSAC 3 cut(s) 902, 1666, 1828
NsbI TGCGCA 1 cut(s) 894
OliI CACNNNNGTG 1 cut(s) 1427
PciSI GCTCTTC 2 cut(s) 4, 1320
PcsI WCGNNNNNNNCGW 1 cut(s) 1168
PctI GAATGC 1 cut(s) 1803
PdiI GCCGGC 1 cut(s) 600
PdmI GAANNNNTTC 1 cut(s) 864
PfeI GAWTC 3 cut(s) 1186, 1289, 1988
PflMI CCANNNNNTGG 2 cut(s) 326, 1880
PkrI GCNGC 9 cut(s) 562, 565, 694, 697, 786, 892, 1598, 1740, 2024
Ple19I CGATCG 1 cut(s) 727
PleI GAGTC 1 cut(s) 1961
PmaCI CACGTG 1 cut(s) 263
PmlI CACGTG 1 cut(s) 263
PpsI GAGTC 1 cut(s) 1961
Ppu21I YACGTR 1 cut(s) 263
PsiI TTATAA 1 cut(s) 410
Psp124BI GAGCTC 2 cut(s) 1171, 1251
Psp6I CCWGG 2 cut(s) 1360, 1614
PspCI CACGTG 1 cut(s) 263
PspGI CCWGG 2 cut(s) 1360, 1614
PspN4I GGNNCC 2 cut(s) 73, 231
PspPI GGNCC 3 cut(s) 682, 916, 1223
PstI CTGCAG 1 cut(s) 778
PsuI RGATCY 2 cut(s) 229, 651
PvuI CGATCG 1 cut(s) 727
PvuII CAGCTG 3 cut(s) 695, 1393, 1741
RgaI GCGATCGC 1 cut(s) 727
RsaI GTAC 3 cut(s) 27, 442, 449
RsaNI GTAC 3 cut(s) 26, 441, 448
RseI CAYNNNNRTG 1 cut(s) 1427
SacI GAGCTC 2 cut(s) 1171, 1251
SapI GCTCTTC 2 cut(s) 4, 1320
SatI GCNGC 9 cut(s) 561, 564, 693, 696, 785, 891, 1597, 1739, 2023
Sau3AI GATC 6 cut(s) 229, 651, 724, 756, 1192, 1953
Sau96I GGNCC 3 cut(s) 682, 916, 1223
SchI GAGTC 1 cut(s) 1961
ScrFI CCNGG 2 cut(s) 1362, 1616
SduI GDGCHC 3 cut(s) 1171, 1251, 1332
SfaAI GCGATCGC 1 cut(s) 727
SfaNI GCATC 5 cut(s) 468, 525, 568, 1467, 1929
SfcI CTRYAG 2 cut(s) 774, 1527
SgfI GCGATCGC 1 cut(s) 727
SinI GGWCC 2 cut(s) 916, 1223
SmiMI CAYNNNNRTG 1 cut(s) 1427
SmlI CTYRAG 6 cut(s) 813, 920, 1052, 1088, 1490, 1802
SmoI CTYRAG 6 cut(s) 813, 920, 1052, 1088, 1490, 1802
SpeI ACTAGT 2 cut(s) 1040, 1546
SsiI CCGC 5 cut(s) 46, 286, 1114, 1386, 1597
SspI AATATT 1 cut(s) 829
SspMI CTAG 7 cut(s) 15, 179, 209, 1041, 1269, 1547, 1979
SstI GAGCTC 2 cut(s) 1171, 1251
StyD4I CCNGG 2 cut(s) 1360, 1614
StyI CCWWGG 3 cut(s) 65, 1620, 1926
TaaI ACNGT 1 cut(s) 452
TaiI ACGT 4 cut(s) 265, 364, 1562, 1758
TaqI TCGA 7 cut(s) 528, 637, 759, 1165, 1305, 1693, 1986
TauI GCSGC 1 cut(s) 1599
TfiI GAWTC 3 cut(s) 1186, 1289, 1988
TscAI CASTG 1 cut(s) 1582
TseFI GTSAC 3 cut(s) 902, 1666, 1828
TseI GCWGC 8 cut(s) 560, 563, 692, 695, 784, 890, 1738, 2022
Tsp45I GTSAC 3 cut(s) 902, 1666, 1828
TspRI CASTG 1 cut(s) 1582
Van91I CCANNNNNTGG 2 cut(s) 326, 1880
Vha464I CTTAAG 1 cut(s) 920
VpaK11BI GGWCC 2 cut(s) 916, 1223
XapI RAATTY 6 cut(s) 91, 123, 273, 385, 586, 1464
XbaI TCTAGA 2 cut(s) 178, 1978
XmiI GTMKAC 1 cut(s) 671
XmnI GAANNNNTTC 1 cut(s) 864
XspI CTAG 7 cut(s) 15, 179, 209, 1041, 1269, 1547, 1979
ZraI GACGTC 1 cut(s) 1560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.