RLG00000008791

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008218
Physical Location & Seq
Reverse (-)
39246087 .. 39249808
3722 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008791

Sequence Viewer

Length: 2850 bp
ATGGCTAGGGTTTTGGCTCTGTCTTCAACAAATAATATTCCTTGTGTGGTTGGTGGGCTAGTTAGGACTGCTCAATCTAAAGAATTGGGTGGAAAAAAGAGTAGTAGAGTGAAGATGATGTGTACTTCTTCACTAGTAGGAGGAGCACTTGCTTTGAGAATGACGTGCATTAGTAGTAGAACTAGTACTGCTTCTAGTTTCTCAGGACTCCGTAGGTTTAACGCCCTAGATACGCTAGTGGCCACACCTAGCAGCCTACACCACGAGGAATACTCTTTTGGAATTGCAACCCCTTGGGGTTCCGAAAGTCGGAAGGGGGCTCGAAGTGGAAGAGCTAGCAGATGTGTACCTAAAGCCATGTTTGAGCGGTTCACAGACAAAGCAATTAAAGTGGTTCTGCTTGCACAAGAGGAATCAAGACGACTTGGTCACAATTTCGTGGGCACTGAGCAGATCCTTTTGGGTCTCATTGGTGAAGGAACTGGTCTTTCTTCCAAGGTTCTGAAATCCTCCATGGGAATTAATCTTAAAGAGGCACGAATTCAAGTGGAGAAGATAATTGGAAGGGGTTCTGGATTTGTTGGTAGTGAGATCCCATTCACTCCCCGTGCCAAACGAGTTTTGGAACTCTCACTCGAAGAGGCTCGCCAACTTGGCCATAACTACATTGGATCCGAACACTTGCTTCTGGGATTGCTTCGAGAGGGGGAGGGTGTAGCAGCAGTTGTTCTAGCGAATTCTGGTGTCGATCCTTGTAATATTCGCACACAGGTTATCCATATGGTGGGCAGTGAGAACACAGCAGAAGCTGTTGTTGATCATAGGCGAGGGGATGATAAGACTAAAATGCCAACACTAGAGGAGTATGGGACCAATTTAACGAAGCTAGCAGAAGAGGGTAAGTTGGATCCTGTTGTTGGAAGAGAACAACAAATATCACGTGTCATCCAAATTTTGGGTAGGCGGACTAAGAATAATCCCTGCCTTATTGGAGAACCTGGTGTTGGGAAGACTGCAATTGCAGAAGGTCTTGCTCAACGTATTGCAAGTGGCGATGCTCCAGAAACAATAGCCAAAAAGACGGTTATAACTCTAGATACGGGTCTTCTTGTGTCTGGTACCAAGTACCGCGGAGAGTTTGAGGGAAGATTGAAGTTGCTAATGGAGGAATTAAAAAAAAGTGGGGGTGAAGTTATTCTGTTTATCGATGAGGTGCACACTTTAATTGGAGCAGGAGCAGCAGAAGGAGCAATTGATGCTGCAAATTTATTGAAGCCGGCCCTTGCAAGAGGTGAACTGCAGTGCATTGGAGCCACAACACTAGATGAATACAGAAAGCATATTGAAAAAGACCCAGCATTGGAAAGACGATTCCAACCAGTCAAAGTTCCTGAACCAACGGTGGATGAAACCATACAGGTTTTGAGAGGGCTTCGAGAACGATATGAGATTCACCACAAAGTGCAGTATACTGATGAGGCCCTGAAAGCAGCTGCGCAGCTATCATACCAATACATCAGCGATCGCTTTCTTCCTGATAAAGCAATTGACTTGCTCGATGAAGCTGGTTCTACAGTACGGCTCCGTCATGCACAGCTTCCTGAAGAAGCTCAAGAGCTTGACAATATTCTGAGGCAGATAACAAAAGAGAAGAATGAAGCTAGTCGTAGCCAAGATTTTGAGAAGGCTGGGAAATTACGCGACAGAGAATTGGACCTTAGGACACAGATCTCAGCAGTAACAGAGAAAGGCAAGGAGATAAGTGAGGCATTGGATTATGTTAATGGTGGTCCCATTGTTACTGAATTGGATATTCAACATATTTTGTCTTGTTGGACTGGTATTCCAGTTGAGAAAGTGTCAACAAATGAATCTGATCGCCTCCTCAAGATGGAAGAGACGCTCCATAAGCGGGTTATTGGCCAGGATGAAGCAGTCAGGGCTATTAGCAGGGCTATTCGTCGAGCTCGTGTTGGACTGAAGAGTCCTAATCGGCCAATTGCCAGTTTCATCTTTTCTGGTCCAACTGGTGTTGGAAAATCTGAGCTCGCAAAAACTTTGGCGACTTGCTATTTTGGCTCTGAAGAATCAATGGTGAGGCTTGATATGAGTGAGTTCATGGAAAGGCACACTGTTTCCAAGCTCATTGGTTCCCCCCCTGGTTATGTTGGTTACACAGAGGGCGGTCAGCTGGTTGAAGCCGTTCGTCGCCATCCTTACACTCTGGTGCTTTTTGATGAGATTGAGAAGGCTCATCCTGATGTTTTCAACTTGATGCTTCAAATACTTGAGGATGGAAGATTGACTGACAGCAAGGGCAGAACTGTAGATTTCAAGAATACACTCATCATTATGACGTCTAATGTTGGAAGCAGTGTCATTGAGAAAGGAGGCCGTAACATGGGATTTGACCCGGCCAAGGATGAAGACAAGGATAGCAGTTACAAACAAATTAAGAGCTTGGTGACGGAGGAATTGAAACATTACTTTCGACCAGAGTTCTTAAATAGGTTGGATGAAATGATTGTTTTTCGGCAGCTGAGTAAATTGGAGGTAAAGGAAATTGCAGATATAATGCTGAATGAGGTTTATGGAAGGCTCAAGTCCAAAGAGATAGAGCTTCGTGTGACAGAGAGGTTCAAAGACAAAGTGGTTGACGAAGGATACGACCCAAGCTATGGAGCAAGGCCTTTAAGAAGAGCCGTAATGAGACTTTTGGAGGACAGCATGGCTGAAAAGATGCTGGCAAGGGAGATCAAAGAGGGCGATTCAGTTATTGTGGATGTTGATTCTGATGGGAATGTGATTGTGCTCATCAATGGCAGCGCTAAGACACAGATAAAGAAATAG

Protein Analysis

950

Amino Acids

104.81

Weight (kDa)

7.61

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 123 - 246 1.6e-30 Clp repeat (R) N-terminal domain
Clp_N PF02861 212 - 267 7.2e-11 Clp repeat (R) N-terminal domain
AAA_16 PF13191 304 - 350 9.8e-06 AAA ATPase domain
AAA PF00004 328 - 441 6.6e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 468 - 568 1.5e-34 AAA lid domain
UVR PF02151 542 - 576 4.7e-09 UvrB/uvrC motif
AAA_2 PF07724 665 - 840 4.7e-67 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 670 - 791 4e-11 AAA domain (dynein-related subfamily)
ClpB_D2-small PF10431 847 - 927 5.1e-25 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1088
AasI GACNNNNNNGTC 2 cut(s) 426, 1983
AatII GACGTC 1 cut(s) 2360
Acc16I TGCGCA 1 cut(s) 1497
Acc65I GGTACC 1 cut(s) 1118
AccB1I GGYRCC 1 cut(s) 1118
AccB7I CCANNNNNTGG 3 cut(s) 784, 955, 2678
AccBSI CCGCTC 1 cut(s) 367
AccI GTMKAC 1 cut(s) 1469
AccII CGCG 2 cut(s) 1131, 1701
AciI CCGC 6 cut(s) 367, 964, 1129, 1131, 1912, 2184
AclWI GGATC 7 cut(s) 448, 586, 666, 679, 743, 902, 915
AcoI YGGCCR 5 cut(s) 240, 655, 1921, 1994, 2415
AcsI RAATTY 4 cut(s) 540, 736, 951, 1264
AcuI CTGAAG 3 cut(s) 1623, 2000, 2103
AcvI CACGTG 1 cut(s) 941
AcyI GRCGYC 1 cut(s) 2357
AdeI CACNNNGTG 1 cut(s) 1462
AfaI GTAC 6 cut(s) 124, 187, 348, 1120, 1127, 1578
AfeI AGCGCT 1 cut(s) 2827
AflIII ACRYGT 1 cut(s) 940
AhlI ACTAGT 2 cut(s) 133, 182
AjiI CACGTC 1 cut(s) 165
AjnI CCWGG 3 cut(s) 997, 1923, 2158
AjuI GAANNNNNNNTTGG 4 cut(s) 261, 293, 987, 1019
AleI CACNNNNGTG 1 cut(s) 2225
Alw21I GWGCWC 5 cut(s) 148, 1218, 1969, 2049, 2814
Alw26I GTCTC 3 cut(s) 470, 1892, 2704
Alw44I GTGCAC 1 cut(s) 1214
AlwI GGATC 7 cut(s) 448, 586, 666, 679, 743, 902, 915
AlwNI CAGNNNCTG 1 cut(s) 809
Aor51HI AGCGCT 1 cut(s) 2827
AoxI GGCC 9 cut(s) 240, 655, 1278, 1479, 1921, 1994, 2392, 2415, 2687
ApaLI GTGCAC 1 cut(s) 1214
ApeKI GCWGC 9 cut(s) 252, 719, 1238, 1259, 1490, 1493, 1498, 2536, 2823
ApoI RAATTY 4 cut(s) 540, 736, 951, 1264
AseI ATTAAT 1 cut(s) 522
AsiSI GCGATCGC 1 cut(s) 1525
Asp700I GAANNNNTTC 3 cut(s) 568, 1194, 2202
Asp718I GGTACC 1 cut(s) 1118
AspLEI GCGC 2 cut(s) 1498, 2828
AspS9I GGNCC 6 cut(s) 870, 1279, 1480, 1714, 1790, 2021
AsuC2I CCSGG 1 cut(s) 2414
AsuHPI GGTGA 6 cut(s) 485, 1199, 1304, 1445, 2107, 2476
AsuNHI GCTAGC 2 cut(s) 335, 886
AvaII GGWCC 4 cut(s) 870, 1714, 1790, 2021
AxyI CCTNAGG 1 cut(s) 1718
BaeGI GKGCMC 2 cut(s) 446, 1218
BaeI ACNNNNGTAYC 2 cut(s) 222, 255
BalI TGGCCA 3 cut(s) 242, 657, 1923
BamHI GGATCC 2 cut(s) 671, 907
BanI GGYRCC 1 cut(s) 1118
BanII GRGCYC 3 cut(s) 322, 1969, 2049
BauI CACGAG 2 cut(s) 263, 1968
BbrPI CACGTG 1 cut(s) 941
BbsI GAAGAC 4 cut(s) 15, 1016, 1097, 2433
Bbv12I GWGCWC 5 cut(s) 148, 1218, 1969, 2049, 2814
BbvI GCAGC 9 cut(s) 264, 731, 1246, 1250, 1480, 1502, 1510, 2548, 2835
BccI CCATC 4 cut(s) 1885, 2220, 2288, 2789
BceAI ACGGC 4 cut(s) 1595, 2186, 2379, 2687
BciT130I CCWGG 3 cut(s) 999, 1925, 2160
BciVI GTATCC 1 cut(s) 2657
BclI TGATCA 1 cut(s) 817
BcnI CCSGG 1 cut(s) 2414
BcoDI GTCTC 3 cut(s) 470, 1892, 2704
BcuI ACTAGT 2 cut(s) 133, 182
BfmI CTRYAG 3 cut(s) 1298, 1572, 2325
BfoI RGCGCY 1 cut(s) 2829
BfuI GTATCC 1 cut(s) 2657
BglI GCCNNNNNGGC 1 cut(s) 654
BglII AGATCT 1 cut(s) 1728
BisI GCNGC 9 cut(s) 253, 720, 1239, 1260, 1491, 1494, 1499, 2537, 2824
BlsI GCNGC 9 cut(s) 254, 721, 1240, 1261, 1492, 1495, 1500, 2538, 2825
BmcAI AGTACT 1 cut(s) 187
Bme1390I CCNGG 4 cut(s) 999, 1925, 2160, 2414
Bme18I GGWCC 4 cut(s) 870, 1714, 1790, 2021
BmgBI CACGTC 1 cut(s) 165
BmgT120I GGNCC 6 cut(s) 870, 1279, 1480, 1714, 1790, 2021
BmiI GGNNCC 9 cut(s) 301, 673, 871, 909, 1120, 1312, 1583, 1792, 2152
BmrFI CCNGG 4 cut(s) 999, 1925, 2160, 2414
BmsI GCATC 4 cut(s) 1045, 1246, 2265, 2730
BmtI GCTAGC 2 cut(s) 339, 890
BpiI GAAGAC 4 cut(s) 15, 1016, 1097, 2433
BplI GAGNNNNNCTC 2 cut(s) 257, 289
BpmI CTGGAG 1 cut(s) 1044
BpuEI CTTGAG 4 cut(s) 1596, 1871, 2309, 2585
BpuMI CCSGG 1 cut(s) 2414
Bsa29I ATCGAT 1 cut(s) 1206
BsaAI YACGTR 1 cut(s) 941
BsaHI GRCGYC 1 cut(s) 2357
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 6 cut(s) 293, 495, 513, 1129, 2158, 2418
Bse118I RCCGGY 1 cut(s) 1276
Bse1I ACTGG 6 cut(s) 487, 1379, 1843, 1847, 2004, 2032
Bse21I CCTNAGG 1 cut(s) 1718
BseBI CCWGG 3 cut(s) 999, 1925, 2160
BseCI ATCGAT 1 cut(s) 1206
BseDI CCNNGG 6 cut(s) 293, 495, 513, 1129, 2158, 2418
BseMII CTCAG 6 cut(s) 216, 438, 1622, 1746, 2034, 2531
BseNI ACTGG 6 cut(s) 487, 1379, 1843, 1847, 2004, 2032
BseRI GAGGAG 3 cut(s) 156, 875, 1874
BseSI GKGCMC 2 cut(s) 446, 1218
BseXI GCAGC 9 cut(s) 264, 731, 1246, 1250, 1480, 1502, 1510, 2548, 2835
BseYI CCCAGC 2 cut(s) 1354, 1688
BsgI GTGCAG 1 cut(s) 1484
Bsh1236I CGCG 2 cut(s) 1131, 1701
Bsh1285I CGRYCG 1 cut(s) 1525
BshFI GGCC 9 cut(s) 242, 657, 1280, 1481, 1923, 1996, 2394, 2417, 2689
BshNI GGYRCC 1 cut(s) 1118
BshVI ATCGAT 1 cut(s) 1206
BsiEI CGRYCG 1 cut(s) 1525
BsiHKAI GWGCWC 5 cut(s) 148, 1218, 1969, 2049, 2814
BsiSI CCGG 2 cut(s) 1277, 2414
BslFI GGGAC 2 cut(s) 883, 1776
BsmAI GTCTC 3 cut(s) 470, 1892, 2704
BsmBI CGTCTC 1 cut(s) 1892
BsmFI GGGAC 2 cut(s) 883, 1776
BsnI GGCC 9 cut(s) 242, 657, 1280, 1481, 1923, 1996, 2394, 2417, 2689
Bso31I GGTCTC 1 cut(s) 470
Bsp1286I GDGCHC 7 cut(s) 148, 322, 446, 1218, 1969, 2049, 2814
Bsp19I CCATGG 1 cut(s) 513
BspACI CCGC 6 cut(s) 367, 964, 1129, 1131, 1912, 2184
BspANI GGCC 9 cut(s) 242, 657, 1280, 1481, 1923, 1996, 2394, 2417, 2689
BspCNI CTCAG 6 cut(s) 215, 439, 1623, 1745, 2035, 2532
BspDI ATCGAT 1 cut(s) 1206
BspFNI CGCG 2 cut(s) 1131, 1701
BspLI GGNNCC 9 cut(s) 301, 673, 871, 909, 1120, 1312, 1583, 1792, 2152
BspMAI CTGCAG 1 cut(s) 1302
BspOI GCTAGC 2 cut(s) 339, 890
BspPI GGATC 7 cut(s) 448, 586, 666, 679, 743, 902, 915
BspQI GCTCTTC 2 cut(s) 325, 2692
BspT107I GGYRCC 1 cut(s) 1118
BspTNI GGTCTC 1 cut(s) 470
BsrBI CCGCTC 1 cut(s) 367
BsrFI RCCGGY 1 cut(s) 1276
BsrI ACTGG 6 cut(s) 487, 1379, 1843, 1847, 2004, 2032
BssAI RCCGGY 1 cut(s) 1276
BssECI CCNNGG 6 cut(s) 293, 495, 513, 1129, 2158, 2418
BssNAI GTATAC 1 cut(s) 1470
BssNI GRCGYC 1 cut(s) 2357
BssSI CACGAG 2 cut(s) 263, 1968
BssT1I CCWWGG 4 cut(s) 293, 495, 513, 2418
Bst1107I GTATAC 1 cut(s) 1470
Bst2BI CACGAG 2 cut(s) 263, 1968
Bst2UI CCWGG 3 cut(s) 999, 1925, 2160
Bst4CI ACNGT 5 cut(s) 1084, 1402, 1576, 2134, 2326
Bst6I CTCTTC 7 cut(s) 325, 633, 888, 916, 1890, 1976, 2692
BstACI GRCGYC 1 cut(s) 2357
BstAPI GCANNNNNTGC 1 cut(s) 1256
BstBAI YACGTR 1 cut(s) 941
BstC8I GCNNGC 7 cut(s) 337, 402, 646, 888, 1278, 2049, 2745
BstDEI CTNAG 9 cut(s) 202, 447, 969, 1631, 1718, 1732, 2043, 2540, 2829
BstDSI CCRYGG 2 cut(s) 513, 1129
BstFNI CGCG 2 cut(s) 1131, 1701
BstH2I RGCGCY 1 cut(s) 2829
BstHHI GCGC 2 cut(s) 1498, 2828
BstMAI GTCTC 3 cut(s) 470, 1892, 2704
BstMCI CGRYCG 1 cut(s) 1525
BstMWI GCNNNNNNNGC 8 cut(s) 654, 1238, 1247, 1256, 1487, 1909, 1940, 2076
BstNI CCWGG 3 cut(s) 999, 1925, 2160
BstSCI CCNGG 4 cut(s) 997, 1923, 2158, 2412
BstSFI CTRYAG 3 cut(s) 1298, 1572, 2325
BstSLI GKGCMC 2 cut(s) 446, 1218
BstUI CGCG 2 cut(s) 1131, 1701
BstV1I GCAGC 9 cut(s) 264, 731, 1246, 1250, 1480, 1502, 1510, 2548, 2835
BstV2I GAAGAC 4 cut(s) 15, 1016, 1097, 2433
BstX2I RGATCY 5 cut(s) 453, 591, 671, 907, 1728
BstYI RGATCY 5 cut(s) 453, 591, 671, 907, 1728
BstZ17I GTATAC 1 cut(s) 1470
Bsu15I ATCGAT 1 cut(s) 1206
Bsu36I CCTNAGG 1 cut(s) 1718
BsuI GTATCC 1 cut(s) 2657
BsuRI GGCC 9 cut(s) 242, 657, 1280, 1481, 1923, 1996, 2394, 2417, 2689
BsuTUI ATCGAT 1 cut(s) 1206
BtgI CCRYGG 2 cut(s) 513, 1129
BtgZI GCGATG 1 cut(s) 1068
BtrI CACGTC 1 cut(s) 165
BtsI GCAGTG 3 cut(s) 796, 1307, 2380
BtsIMutI CAGTG 5 cut(s) 444, 796, 1307, 2130, 2380
Cac8I GCNNGC 7 cut(s) 337, 402, 646, 888, 1278, 2049, 2745
CaiI CAGNNNCTG 1 cut(s) 809
CfoI GCGC 2 cut(s) 1498, 2828
Cfr10I RCCGGY 1 cut(s) 1276
Cfr13I GGNCC 6 cut(s) 870, 1279, 1480, 1714, 1790, 2021
Cfr42I CCGCGG 1 cut(s) 1132
ClaI ATCGAT 1 cut(s) 1206
CseI GACGC 1 cut(s) 1909
CsiI ACCWGGT 1 cut(s) 997
Csp6I GTAC 6 cut(s) 123, 186, 347, 1119, 1126, 1577
CspCI CAANNNNNGTGG 2 cut(s) 372, 407
CviAII CATG 6 cut(s) 358, 514, 1589, 2119, 2401, 2728
CviQI GTAC 6 cut(s) 123, 186, 347, 1119, 1126, 1577
DdeI CTNAG 9 cut(s) 202, 447, 969, 1631, 1718, 1732, 2043, 2540, 2829
DraIII CACNNNGTG 1 cut(s) 1462
DrdI GACNNNNNNGTC 2 cut(s) 426, 1983
DseDI GACNNNNNNGTC 2 cut(s) 426, 1983
EaeI YGGCCR 5 cut(s) 240, 655, 1921, 1994, 2415
Eam1104I CTCTTC 7 cut(s) 325, 633, 888, 916, 1890, 1976, 2692
EarI CTCTTC 7 cut(s) 325, 633, 888, 916, 1890, 1976, 2692
EciI GGCGGA 1 cut(s) 979
Ecl136II GAGCTC 2 cut(s) 1967, 2047
Eco130I CCWWGG 4 cut(s) 293, 495, 513, 2418
Eco147I AGGCCT 1 cut(s) 2689
Eco24I GRGCYC 3 cut(s) 322, 1969, 2049
Eco31I GGTCTC 1 cut(s) 470
Eco47I GGWCC 4 cut(s) 870, 1714, 1790, 2021
Eco47III AGCGCT 1 cut(s) 2827
Eco53kI GAGCTC 2 cut(s) 1967, 2047
Eco57I CTGAAG 3 cut(s) 1623, 2000, 2103
Eco72I CACGTG 1 cut(s) 941
Eco81I CCTNAGG 1 cut(s) 1718
EcoICRI GAGCTC 2 cut(s) 1967, 2047
EcoO109I RGGNCCY 1 cut(s) 1480
EcoRI GAATTC 2 cut(s) 540, 736
EcoRII CCWGG 3 cut(s) 997, 1923, 2158
EcoT14I CCWWGG 4 cut(s) 293, 495, 513, 2418
EcoT38I GRGCYC 3 cut(s) 322, 1969, 2049
ErhI CCWWGG 4 cut(s) 293, 495, 513, 2418
Esp3I CGTCTC 1 cut(s) 1892
FaeI CATG 6 cut(s) 361, 517, 1592, 2122, 2404, 2731
FaqI GGGAC 2 cut(s) 883, 1776
FatI CATG 6 cut(s) 357, 513, 1588, 2118, 2400, 2727
FauI CCCGC 1 cut(s) 1905
FauNDI CATATG 1 cut(s) 780
FbaI TGATCA 1 cut(s) 817
FblI GTMKAC 1 cut(s) 1469
Fnu4HI GCNGC 9 cut(s) 253, 720, 1239, 1260, 1491, 1494, 1499, 2537, 2824
FriOI GRGCYC 3 cut(s) 322, 1969, 2049
Fsp4HI GCNGC 9 cut(s) 253, 720, 1239, 1260, 1491, 1494, 1499, 2537, 2824
FspI TGCGCA 1 cut(s) 1497
GlaI GCGC 2 cut(s) 1497, 2827
GluI GCNGC 9 cut(s) 253, 720, 1239, 1260, 1491, 1494, 1499, 2537, 2824
GsaI CCCAGC 2 cut(s) 1358, 1692
GsuI CTGGAG 1 cut(s) 1044
HaeII RGCGCY 1 cut(s) 2829
HaeIII GGCC 9 cut(s) 242, 657, 1280, 1481, 1923, 1996, 2394, 2417, 2689
HapII CCGG 2 cut(s) 1277, 2414
HgaI GACGC 1 cut(s) 1909
HhaI GCGC 2 cut(s) 1498, 2828
Hin1I GRCGYC 1 cut(s) 2357
Hin1II CATG 6 cut(s) 361, 517, 1592, 2122, 2404, 2731
Hin6I GCGC 2 cut(s) 1496, 2826
HinP1I GCGC 2 cut(s) 1496, 2826
HincII GTYRAC 2 cut(s) 1863, 2656
HindII GTYRAC 2 cut(s) 1863, 2656
HinfI GANTC 9 cut(s) 207, 413, 1371, 1450, 1871, 1984, 2087, 2768, 2789
HpaII CCGG 2 cut(s) 1277, 2414
HphI GGTGA 6 cut(s) 485, 1199, 1304, 1445, 2107, 2476
Hpy166II GTNNAC 8 cut(s) 123, 347, 372, 1216, 1295, 1470, 1863, 2656
Hpy188I TCNGA 9 cut(s) 304, 312, 504, 676, 1632, 1876, 2044, 2083, 2794
Hpy8I GTNNAC 8 cut(s) 123, 347, 372, 1216, 1295, 1470, 1863, 2656
Hpy99I CGWCG 2 cut(s) 1965, 2211
HpyAV CCTTC 9 cut(s) 307, 470, 558, 1019, 1238, 1678, 2242, 2589, 2654
HpyCH4III ACNGT 5 cut(s) 1084, 1402, 1576, 2134, 2326
HpyCH4IV ACGT 4 cut(s) 164, 940, 1039, 2357
HpyF10VI GCNNNNNNNGC 8 cut(s) 654, 1238, 1247, 1256, 1487, 1909, 1940, 2076
HpyF3I CTNAG 9 cut(s) 202, 447, 969, 1631, 1718, 1732, 2043, 2540, 2829
HpySE526I ACGT 4 cut(s) 164, 940, 1039, 2357
Hsp92I GRCGYC 1 cut(s) 2357
Hsp92II CATG 6 cut(s) 361, 517, 1592, 2122, 2404, 2731
HspAI GCGC 2 cut(s) 1496, 2826
KpnI GGTACC 1 cut(s) 1122
KroI GCCGGC 1 cut(s) 1276
KroNI GCCGGC 1 cut(s) 1278
Ksp22I TGATCA 1 cut(s) 817
KspI CCGCGG 1 cut(s) 1132
LguI GCTCTTC 2 cut(s) 325, 2692
LmnI GCTCC 9 cut(s) 143, 1063, 1229, 1235, 1247, 1310, 1587, 1908, 2681
Lsp1109I GCAGC 9 cut(s) 264, 731, 1246, 1250, 1480, 1502, 1510, 2548, 2835
LweI GCATC 4 cut(s) 1045, 1246, 2265, 2730
MabI ACCWGGT 1 cut(s) 997
MaeII ACGT 4 cut(s) 164, 940, 1039, 2357
MaeIII GTNAC 8 cut(s) 428, 1738, 1798, 2171, 2396, 2441, 2464, 2626
MbiI CCGCTC 1 cut(s) 367
MfeI CAATTG 4 cut(s) 1017, 1251, 1545, 1998
MflI RGATCY 5 cut(s) 453, 591, 671, 907, 1728
MhlI GDGCHC 7 cut(s) 148, 322, 446, 1218, 1969, 2049, 2814
MlsI TGGCCA 3 cut(s) 242, 657, 1923
MluNI TGGCCA 3 cut(s) 242, 657, 1923
MlyI GAGTC 2 cut(s) 201, 1993
Mox20I TGGCCA 3 cut(s) 242, 657, 1923
MroNI GCCGGC 1 cut(s) 1276
MroXI GAANNNNTTC 3 cut(s) 568, 1194, 2202
MscI TGGCCA 3 cut(s) 242, 657, 1923
MslI CAYNNNNRTG 3 cut(s) 2225, 2259, 2351
Msp20I TGGCCA 3 cut(s) 242, 657, 1923
MspA1I CMGCKG 4 cut(s) 1131, 1493, 2191, 2539
MspI CCGG 2 cut(s) 1277, 2414
MspR9I CCNGG 4 cut(s) 999, 1925, 2160, 2414
MunI CAATTG 4 cut(s) 1017, 1251, 1545, 1998
MvaI CCWGG 3 cut(s) 999, 1925, 2160
MvnI CGCG 2 cut(s) 1131, 1701
MwoI GCNNNNNNNGC 8 cut(s) 654, 1238, 1247, 1256, 1487, 1909, 1940, 2076
NaeI GCCGGC 1 cut(s) 1278
NciI CCSGG 1 cut(s) 2414
NcoI CCATGG 1 cut(s) 513
NdeI CATATG 1 cut(s) 780
NgoMIV GCCGGC 1 cut(s) 1276
NheI GCTAGC 2 cut(s) 335, 886
NlaIII CATG 6 cut(s) 361, 517, 1592, 2122, 2404, 2731
NlaIV GGNNCC 9 cut(s) 301, 673, 871, 909, 1120, 1312, 1583, 1792, 2152
NmuCI GTSAC 3 cut(s) 428, 2464, 2626
NsbI TGCGCA 1 cut(s) 1497
OliI CACNNNNGTG 1 cut(s) 2225
PceI AGGCCT 1 cut(s) 2689
PciSI GCTCTTC 2 cut(s) 325, 2692
PcsI WCGNNNNNNNCGW 2 cut(s) 1966, 2664
PdiI GCCGGC 1 cut(s) 1278
PdmI GAANNNNTTC 3 cut(s) 568, 1194, 2202
PfeI GAWTC 7 cut(s) 413, 1371, 1450, 1871, 2087, 2768, 2789
PflFI GACNNNGTC 1 cut(s) 426
PflMI CCANNNNNTGG 3 cut(s) 784, 955, 2678
PkrI GCNGC 9 cut(s) 254, 721, 1240, 1261, 1492, 1495, 1500, 2538, 2825
Ple19I CGATCG 1 cut(s) 1525
PleI GAGTC 2 cut(s) 201, 1992
PmaCI CACGTG 1 cut(s) 941
PmlI CACGTG 1 cut(s) 941
PpsI GAGTC 2 cut(s) 201, 1992
Ppu21I YACGTR 1 cut(s) 941
PshBI ATTAAT 1 cut(s) 522
PsiI TTATAA 1 cut(s) 1088
Psp124BI GAGCTC 2 cut(s) 1969, 2049
Psp6I CCWGG 3 cut(s) 997, 1923, 2158
PspCI CACGTG 1 cut(s) 941
PspFI CCCAGC 2 cut(s) 1354, 1688
PspGI CCWGG 3 cut(s) 997, 1923, 2158
PspN4I GGNNCC 9 cut(s) 301, 673, 871, 909, 1120, 1312, 1583, 1792, 2152
PspPI GGNCC 6 cut(s) 870, 1279, 1480, 1714, 1790, 2021
PstI CTGCAG 1 cut(s) 1302
PstNI CAGNNNCTG 1 cut(s) 809
PsuI RGATCY 5 cut(s) 453, 591, 671, 907, 1728
PsyI GACNNNGTC 1 cut(s) 426
PvuI CGATCG 1 cut(s) 1525
PvuII CAGCTG 3 cut(s) 1493, 2191, 2539
RgaI GCGATCGC 1 cut(s) 1525
RsaI GTAC 6 cut(s) 124, 187, 348, 1120, 1127, 1578
RsaNI GTAC 6 cut(s) 123, 186, 347, 1119, 1126, 1577
RseI CAYNNNNRTG 3 cut(s) 2225, 2259, 2351
SacI GAGCTC 2 cut(s) 1969, 2049
SacII CCGCGG 1 cut(s) 1132
SapI GCTCTTC 2 cut(s) 325, 2692
SatI GCNGC 9 cut(s) 253, 720, 1239, 1260, 1491, 1494, 1499, 2537, 2824
Sau96I GGNCC 6 cut(s) 870, 1279, 1480, 1714, 1790, 2021
ScaI AGTACT 1 cut(s) 187
SchI GAGTC 2 cut(s) 201, 1993
ScrFI CCNGG 4 cut(s) 999, 1925, 2160, 2414
SduI GDGCHC 7 cut(s) 148, 322, 446, 1218, 1969, 2049, 2814
SexAI ACCWGGT 1 cut(s) 997
SfaAI GCGATCGC 1 cut(s) 1525
SfaNI GCATC 4 cut(s) 1045, 1246, 2265, 2730
SfcI CTRYAG 3 cut(s) 1298, 1572, 2325
Sfr303I CCGCGG 1 cut(s) 1132
SgfI GCGATCGC 1 cut(s) 1525
SgrBI CCGCGG 1 cut(s) 1132
SinI GGWCC 4 cut(s) 870, 1714, 1790, 2021
SmiMI CAYNNNNRTG 3 cut(s) 2225, 2259, 2351
SmlI CTYRAG 4 cut(s) 1611, 1886, 2288, 2600
SmoI CTYRAG 4 cut(s) 1611, 1886, 2288, 2600
SpeI ACTAGT 2 cut(s) 133, 182
SseBI AGGCCT 1 cut(s) 2689
SsiI CCGC 6 cut(s) 367, 964, 1129, 1131, 1912, 2184
SspI AATATT 3 cut(s) 37, 760, 1627
SstI GAGCTC 2 cut(s) 1969, 2049
StuI AGGCCT 1 cut(s) 2689
StyD4I CCNGG 4 cut(s) 997, 1923, 2158, 2412
StyI CCWWGG 4 cut(s) 293, 495, 513, 2418
TaaI ACNGT 5 cut(s) 1084, 1402, 1576, 2134, 2326
TaiI ACGT 4 cut(s) 167, 943, 1042, 2360
TaqI TCGA 9 cut(s) 322, 636, 700, 747, 1206, 1435, 1557, 1963, 2491
TatI WGTACW 2 cut(s) 122, 185
TfiI GAWTC 7 cut(s) 413, 1371, 1450, 1871, 2087, 2768, 2789
TscAI CASTG 5 cut(s) 451, 796, 1307, 2137, 2380
TseFI GTSAC 3 cut(s) 428, 2464, 2626
TseI GCWGC 9 cut(s) 252, 719, 1238, 1259, 1490, 1493, 1498, 2536, 2823
Tsp45I GTSAC 3 cut(s) 428, 2464, 2626
TspGWI ACGGA 3 cut(s) 200, 1574, 2483
TspRI CASTG 5 cut(s) 451, 796, 1307, 2137, 2380
Tth111I GACNNNGTC 1 cut(s) 426
Van91I CCANNNNNTGG 3 cut(s) 784, 955, 2678
VneI GTGCAC 1 cut(s) 1214
VpaK11BI GGWCC 4 cut(s) 870, 1714, 1790, 2021
VspI ATTAAT 1 cut(s) 522
XapI RAATTY 4 cut(s) 540, 736, 951, 1264
XbaI TCTAGA 1 cut(s) 1093
XcmI CCANNNNNNNNNTGG 2 cut(s) 619, 665
XmiI GTMKAC 1 cut(s) 1469
XmnI GAANNNNTTC 3 cut(s) 568, 1194, 2202
ZraI GACGTC 1 cut(s) 2358
ZrmI AGTACT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.