Rh4AG136000

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0008218
Physical Location & Seq
Forward (+)
29870162 .. 29873887
3726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG136000.1

Sequence Viewer

Length: 2853 bp
ATGGCTAGGGTTTTGGCTCTGTCTTCAACAAATAATATTCCTTGTGTGGTTGGTGGGATAGTTAGGACTGCTCAATCTAAAGAATTGGGTGGAAAAAAGAGTAGTAGAGTGAAGATGATGTGTACTTCTTCGCTAGTAGGAGGAGCACTTGCTTTGAGAATGAGGTGCATGAGTAGTAGTAGCACTAGTACTGCTTCTAGTTTCTCAGGACTCCGTAGGTTTAACGCCCTAGATAAGCTGGTGGCCACACCTAGCAGCCTACACCACGAGGAATACTCTTTTGGAATTGCAACCCCTTGGGGTTCCGAAAGTCAGAAGGGGGCTAGAAGTGGAAGAGCTAGCAGATGTGTACCTAAAGCCATGTTTGAGCGGTTCACAGACAAAGCAATTAAAGTGGTTCTGCTTGCACAAGAGGAATCAAGACGACTTGGTCACAATTTCGTGGGCACTGAGCAGATCCTTTTGGGTCTCATTGGTGAAGGAACTGGTCTTCCTTCCAAGGTTCTGAAATCCTCCATGGGAATTAATCTTAAAGAGGCACGAATTCAAGTGGAGAAGATAATTGGAAGGGGTTCTGGATTTATTGCTGTTGAGATCCCATTCACTTCTCGTGCCAAACGAGTTTTGGAACTCTCACTCGAAGAGGCTCGCCAACTTGGCCATAACTACATTGGATCCGAACACTTGCTTCTGGGACTGCTTCGAGAGGGGGAGGGTGTAGCAGCAGTTGTTCTAGCGAATTCTGGTGTCGATCCTTGTAATATTCGCACACAGGTTATCCATATGGTGGGCAGTGAGAACACAGCAGAAGCTGTTGTTGATCATAGGCGAGGGGATGATAAGACTAAAATGCCAACACTAGAGGAGTACGGGACCAATTTAACGAAGCTAGCAGAAGAGGGTAAGTTGGATCCTGTTGTTGGAAGAGAACAACAAATATCACGTGTCATCCAAATTTTGGGTAGGCGGACTAAGAATAATCCCTGCCTTATTGGAGAACCTGGTGTTGGAAAGACTGCAATTGCAGAAGGTCTTGCTCAACGTATTGCAAGTGGCGATGCTCCAGAAACAATAGCCAAAAAGACGGTTATAACTCTTGATACAGGTCTTCTTGTGTCTGGTACCAAGTACCGCGGAGAGTTTGAGGGAAGATTGAAGTTGCTAATGGAGGAATTAAAAAAAAGTGGTGGTGAAGTTATTCTGTTTATCGATGAGGTGCACACTTTAATTGGAGCAGGAGCAGCAGAAGGAGCAATTGATGCTGCAAATTTATTGAAGCCGGCCCTTGCAAGAGGTGAACTGCAGTGCATTGGAGCCACAACACTAGATGAATACAGAAAGCACATTGAAAAAGACCCAGCATTGGAAAGACGATTCCAACCAGTCAAAGTTCCTGAACCAACGGTGGATGAAACCATACAGGTTTTGAGAGGGCTTCGAGAACGATATGAGATTCACCACAAAGTGCAGTATACTGATGAGGCCCTGAAAGCAGCTGCGCAGCTATCATACCAATACATCAGCGATCGCTTTCTTCCTGATAAAGCAATTGACTTGCTCGATGAAGCTGGTTCTACAGTACGGCTCCGTCATGCACAGCTTCCTGAAGAAGCTCAAGAGCTTGACAATATTCTGAGGCAGATAACAAAAGAGAAGAATGAAGCTAGTCGTAGCCAAGATTTTGAGAAGGCTGGGAAATTACGCGACAGAGAATTGGACCTTAAGAAACAGATCTCAGCAGTAACAGAGAAAGGCAAGGAGATAAGTGAGGCATTGGATTATGTTAATGGTGGCCCTATTGTTACTGAATTGGATATTCAACATATTTTGTCTTGTTGGACTGGTATTCCAGTTGAGAAAGTGTCAACAAATGAATCTGATCGCCTCCTCAAGATGGAAGAGACGCTCCATAAGCGGGTTATTGGCCAGGATGAAGCAGTCAGGGCTATTAGCAGGGCTATTCGTCGAGCTCGTGTTGGACTGAAGAGTCCTAATCGGCCAATTGCCAGTTTCATCTTTTCTGGTCCAACTGGTGTTGGAAAATCTGAGCTCGCAAAAACTTTGGCGACTTGCTATTTTGGCTCTGAAGAATCAATGGTGAGGCTTGATATGAGTGAGTTCATGGAAAGGCACACTGTTTCCAAGCTCATTGGTTCCCCCCCTGGTTATGTTGGTTACACAGAGGGCGGTCAGCTGGTTGAAGCCGTTCGTCGCCATCCATACACTCTGGTGCTTTTTGATGAGATTGAGAAGGCTCATCCTGATGTTTTCAACTTGATGCTTCAAATACTTGAGGATGGAAGATTGACTGACAGCAAGGGCAGAACAGTAGATTTCAAGAATACACTCATCATTATGACGTCTAATGTTGGAAGCAGTGTCATTGAGAAAGGAGGCGGTAACATGGGATTTGACCCGGCCAAGGATGAAGACAAGGATAGCAGTTACAAACAAATTAAGAGCTTGGTGATGGAGGAATTGAAACACTACTTTCGACCAGAGTTCTTAAATAGGTTGGATGAAATGATTGTTTTTCGGCAGCTGTGTAAGTTGGAGGTAAAGGAAATTGCAGATATAATGCTGAACGAGGTTTATGGAAGGCTCAAGTCCAAAGAGATAGAGCTTCGTGTGACAGAGAGGTTCAAAGACAAAGTGGTTGATGAAGGATACGACCCAAGCTATGGAGCAAGGCCTTTAAGAAGAGCCATAATGAGACTTTTGGAGGACAGCATGGCTGAAAAGATGCTGGCAAGGGAGATCAAAGATGGTGATTCAGTTATTGTGGATGTTGATTCTGATGGGAATGTGATTGTGCTCATCAATGGTAGCGCTAAGACAGAGATAAAGAAATAG

Protein Analysis

950

Amino Acids

104.89

Weight (kDa)

7.28

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 124 - 247 2.6e-28 Clp repeat (R) N-terminal domain
Clp_N PF02861 201 - 269 7e-15 Clp repeat (R) N-terminal domain
AAA_16 PF13191 305 - 351 9.8e-06 AAA ATPase domain
AAA PF00004 329 - 442 6.6e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 469 - 569 1.5e-34 AAA lid domain
UVR PF02151 543 - 577 3.1e-09 UvrB/uvrC motif
AAA_2 PF07724 666 - 841 4.2e-67 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 671 - 792 4.2e-11 AAA domain (dynein-related subfamily)
ClpB_D2-small PF10431 849 - 928 3.7e-25 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1091
AasI GACNNNNNNGTC 2 cut(s) 429, 1986
AatII GACGTC 1 cut(s) 2363
Acc16I TGCGCA 1 cut(s) 1500
Acc65I GGTACC 1 cut(s) 1121
AccB1I GGYRCC 1 cut(s) 1121
AccB7I CCANNNNNTGG 3 cut(s) 787, 958, 2681
AccBSI CCGCTC 1 cut(s) 370
AccI GTMKAC 1 cut(s) 1472
AccII CGCG 2 cut(s) 1134, 1704
AciI CCGC 7 cut(s) 370, 967, 1132, 1134, 1915, 2187, 2397
AclWI GGATC 7 cut(s) 451, 589, 669, 682, 746, 905, 918
AcoI YGGCCR 5 cut(s) 243, 658, 1924, 1997, 2418
AcsI RAATTY 4 cut(s) 543, 739, 954, 1267
AcuI CTGAAG 3 cut(s) 1626, 2003, 2106
AcvI CACGTG 1 cut(s) 944
AcyI GRCGYC 1 cut(s) 2360
AdeI CACNNNGTG 1 cut(s) 1465
AfaI GTAC 7 cut(s) 124, 190, 351, 869, 1123, 1130, 1581
AfeI AGCGCT 1 cut(s) 2830
AfiI CCNNNNNNNGG 9 cut(s) 787, 920, 958, 1007, 1363, 1894, 1915, 2422, 2681
AflII CTTAAG 1 cut(s) 1721
AflIII ACRYGT 1 cut(s) 943
AhlI ACTAGT 1 cut(s) 185
AjnI CCWGG 3 cut(s) 1000, 1926, 2161
AjuI GAANNNNNNNTTGG 4 cut(s) 264, 296, 990, 1022
AleI CACNNNNGTG 1 cut(s) 2228
AloI GAACNNNNNNTCC 2 cut(s) 475, 507
Alw21I GWGCWC 5 cut(s) 148, 1221, 1972, 2052, 2817
Alw26I GTCTC 3 cut(s) 473, 1895, 2707
Alw44I GTGCAC 1 cut(s) 1217
AlwI GGATC 7 cut(s) 451, 589, 669, 682, 746, 905, 918
AlwNI CAGNNNCTG 1 cut(s) 812
Aor51HI AGCGCT 1 cut(s) 2830
AoxI GGCC 9 cut(s) 243, 658, 1281, 1482, 1792, 1924, 1997, 2418, 2690
ApaLI GTGCAC 1 cut(s) 1217
ApeKI GCWGC 8 cut(s) 255, 722, 1241, 1262, 1493, 1496, 1501, 2539
ApoI RAATTY 4 cut(s) 543, 739, 954, 1267
AseI ATTAAT 1 cut(s) 525
AsiSI GCGATCGC 1 cut(s) 1528
Asp700I GAANNNNTTC 3 cut(s) 571, 1197, 2205
Asp718I GGTACC 1 cut(s) 1121
AspLEI GCGC 2 cut(s) 1501, 2831
AspS9I GGNCC 6 cut(s) 873, 1282, 1483, 1717, 1793, 2024
AsuC2I CCSGG 1 cut(s) 2417
AsuHPI GGTGA 7 cut(s) 488, 1202, 1307, 1448, 2110, 2479, 2780
AsuNHI GCTAGC 2 cut(s) 338, 889
AvaII GGWCC 3 cut(s) 873, 1717, 2024
BaeGI GKGCMC 2 cut(s) 449, 1221
BalI TGGCCA 3 cut(s) 245, 660, 1926
BamHI GGATCC 2 cut(s) 674, 910
BanI GGYRCC 1 cut(s) 1121
BanII GRGCYC 2 cut(s) 1972, 2052
BauI CACGAG 3 cut(s) 266, 609, 1971
BbrPI CACGTG 1 cut(s) 944
BbsI GAAGAC 4 cut(s) 15, 482, 1100, 2436
Bbv12I GWGCWC 5 cut(s) 148, 1221, 1972, 2052, 2817
BbvI GCAGC 8 cut(s) 267, 734, 1249, 1253, 1483, 1505, 1513, 2551
BccI CCATC 6 cut(s) 1888, 2223, 2291, 2464, 2759, 2792
BceAI ACGGC 2 cut(s) 1598, 2189
BciT130I CCWGG 3 cut(s) 1002, 1928, 2163
BciVI GTATCC 1 cut(s) 2660
BclI TGATCA 1 cut(s) 820
BcnI CCSGG 1 cut(s) 2417
BcoDI GTCTC 3 cut(s) 473, 1895, 2707
BcuI ACTAGT 1 cut(s) 185
BfmI CTRYAG 2 cut(s) 1301, 1575
BfoI RGCGCY 1 cut(s) 2832
BfrI CTTAAG 1 cut(s) 1721
BfuI GTATCC 1 cut(s) 2660
BglI GCCNNNNNGGC 1 cut(s) 657
BglII AGATCT 1 cut(s) 1731
BisI GCNGC 8 cut(s) 256, 723, 1242, 1263, 1494, 1497, 1502, 2540
BlsI GCNGC 8 cut(s) 257, 724, 1243, 1264, 1495, 1498, 1503, 2541
BmcAI AGTACT 1 cut(s) 190
Bme1390I CCNGG 4 cut(s) 1002, 1928, 2163, 2417
Bme18I GGWCC 3 cut(s) 873, 1717, 2024
BmgT120I GGNCC 6 cut(s) 873, 1282, 1483, 1717, 1793, 2024
BmiI GGNNCC 8 cut(s) 304, 676, 874, 912, 1123, 1315, 1586, 2155
BmrFI CCNGG 4 cut(s) 1002, 1928, 2163, 2417
BmsI GCATC 4 cut(s) 1048, 1249, 2268, 2733
BmtI GCTAGC 2 cut(s) 342, 893
BpiI GAAGAC 4 cut(s) 15, 482, 1100, 2436
BplI GAGNNNNNCTC 2 cut(s) 260, 292
BpmI CTGGAG 1 cut(s) 1047
BpuEI CTTGAG 4 cut(s) 1599, 1874, 2312, 2588
BpuMI CCSGG 1 cut(s) 2417
Bsa29I ATCGAT 1 cut(s) 1209
BsaAI YACGTR 1 cut(s) 944
BsaHI GRCGYC 1 cut(s) 2360
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 6 cut(s) 296, 498, 516, 1132, 2161, 2421
Bsc4I CCNNNNNNNGG 9 cut(s) 787, 920, 958, 1007, 1363, 1894, 1915, 2422, 2681
Bse118I RCCGGY 1 cut(s) 1279
Bse1I ACTGG 6 cut(s) 490, 1382, 1846, 1850, 2007, 2035
BseBI CCWGG 3 cut(s) 1002, 1928, 2163
BseCI ATCGAT 1 cut(s) 1209
BseDI CCNNGG 6 cut(s) 296, 498, 516, 1132, 2161, 2421
BseLI CCNNNNNNNGG 9 cut(s) 787, 920, 958, 1007, 1363, 1894, 1915, 2422, 2681
BseMII CTCAG 5 cut(s) 219, 441, 1625, 1749, 2037
BseNI ACTGG 6 cut(s) 490, 1382, 1846, 1850, 2007, 2035
BseRI GAGGAG 3 cut(s) 156, 878, 1877
BseSI GKGCMC 2 cut(s) 449, 1221
BseXI GCAGC 8 cut(s) 267, 734, 1249, 1253, 1483, 1505, 1513, 2551
BseYI CCCAGC 2 cut(s) 1357, 1691
BsgI GTGCAG 1 cut(s) 1487
Bsh1236I CGCG 2 cut(s) 1134, 1704
Bsh1285I CGRYCG 1 cut(s) 1528
BshFI GGCC 9 cut(s) 245, 660, 1283, 1484, 1794, 1926, 1999, 2420, 2692
BshNI GGYRCC 1 cut(s) 1121
BshVI ATCGAT 1 cut(s) 1209
BsiEI CGRYCG 1 cut(s) 1528
BsiHKAI GWGCWC 5 cut(s) 148, 1221, 1972, 2052, 2817
BsiSI CCGG 2 cut(s) 1280, 2417
BslFI GGGAC 2 cut(s) 708, 886
BslI CCNNNNNNNGG 9 cut(s) 787, 920, 958, 1007, 1363, 1894, 1915, 2422, 2681
BsmAI GTCTC 3 cut(s) 473, 1895, 2707
BsmBI CGTCTC 1 cut(s) 1895
BsmFI GGGAC 2 cut(s) 708, 886
BsnI GGCC 9 cut(s) 245, 660, 1283, 1484, 1794, 1926, 1999, 2420, 2692
Bso31I GGTCTC 1 cut(s) 473
Bsp1286I GDGCHC 6 cut(s) 148, 449, 1221, 1972, 2052, 2817
Bsp19I CCATGG 1 cut(s) 516
BspACI CCGC 7 cut(s) 370, 967, 1132, 1134, 1915, 2187, 2397
BspANI GGCC 9 cut(s) 245, 660, 1283, 1484, 1794, 1926, 1999, 2420, 2692
BspCNI CTCAG 5 cut(s) 218, 442, 1626, 1748, 2038
BspDI ATCGAT 1 cut(s) 1209
BspFNI CGCG 2 cut(s) 1134, 1704
BspLI GGNNCC 8 cut(s) 304, 676, 874, 912, 1123, 1315, 1586, 2155
BspMAI CTGCAG 1 cut(s) 1305
BspOI GCTAGC 2 cut(s) 342, 893
BspPI GGATC 7 cut(s) 451, 589, 669, 682, 746, 905, 918
BspQI GCTCTTC 2 cut(s) 328, 2695
BspT107I GGYRCC 1 cut(s) 1121
BspTI CTTAAG 1 cut(s) 1721
BspTNI GGTCTC 1 cut(s) 473
BsrBI CCGCTC 1 cut(s) 370
BsrFI RCCGGY 1 cut(s) 1279
BsrI ACTGG 6 cut(s) 490, 1382, 1846, 1850, 2007, 2035
BssAI RCCGGY 1 cut(s) 1279
BssECI CCNNGG 6 cut(s) 296, 498, 516, 1132, 2161, 2421
BssNAI GTATAC 1 cut(s) 1473
BssNI GRCGYC 1 cut(s) 2360
BssSI CACGAG 3 cut(s) 266, 609, 1971
BssT1I CCWWGG 4 cut(s) 296, 498, 516, 2421
Bst1107I GTATAC 1 cut(s) 1473
Bst2BI CACGAG 3 cut(s) 266, 609, 1971
Bst2UI CCWGG 3 cut(s) 1002, 1928, 2163
Bst4CI ACNGT 5 cut(s) 1087, 1405, 1579, 2137, 2329
Bst6I CTCTTC 7 cut(s) 328, 636, 891, 919, 1893, 1979, 2695
BstACI GRCGYC 1 cut(s) 2360
BstAFI CTTAAG 1 cut(s) 1721
BstAPI GCANNNNNTGC 1 cut(s) 1259
BstBAI YACGTR 1 cut(s) 944
BstC8I GCNNGC 7 cut(s) 340, 405, 649, 891, 1281, 2052, 2748
BstDEI CTNAG 7 cut(s) 205, 450, 972, 1634, 1735, 2046, 2832
BstDSI CCRYGG 2 cut(s) 516, 1132
BstFNI CGCG 2 cut(s) 1134, 1704
BstH2I RGCGCY 1 cut(s) 2832
BstHHI GCGC 2 cut(s) 1501, 2831
BstMAI GTCTC 3 cut(s) 473, 1895, 2707
BstMCI CGRYCG 1 cut(s) 1528
BstMWI GCNNNNNNNGC 8 cut(s) 657, 1241, 1250, 1259, 1490, 1912, 1943, 2079
BstNI CCWGG 3 cut(s) 1002, 1928, 2163
BstSCI CCNGG 4 cut(s) 1000, 1926, 2161, 2415
BstSFI CTRYAG 2 cut(s) 1301, 1575
BstSLI GKGCMC 2 cut(s) 449, 1221
BstUI CGCG 2 cut(s) 1134, 1704
BstV1I GCAGC 8 cut(s) 267, 734, 1249, 1253, 1483, 1505, 1513, 2551
BstV2I GAAGAC 4 cut(s) 15, 482, 1100, 2436
BstX2I RGATCY 5 cut(s) 456, 594, 674, 910, 1731
BstYI RGATCY 5 cut(s) 456, 594, 674, 910, 1731
BstZ17I GTATAC 1 cut(s) 1473
Bsu15I ATCGAT 1 cut(s) 1209
BsuI GTATCC 1 cut(s) 2660
BsuRI GGCC 9 cut(s) 245, 660, 1283, 1484, 1794, 1926, 1999, 2420, 2692
BsuTUI ATCGAT 1 cut(s) 1209
BtgI CCRYGG 2 cut(s) 516, 1132
BtgZI GCGATG 1 cut(s) 1071
BtsI GCAGTG 3 cut(s) 799, 1310, 2383
BtsIMutI CAGTG 5 cut(s) 447, 799, 1310, 2133, 2383
Cac8I GCNNGC 7 cut(s) 340, 405, 649, 891, 1281, 2052, 2748
CaiI CAGNNNCTG 1 cut(s) 812
CfoI GCGC 2 cut(s) 1501, 2831
Cfr10I RCCGGY 1 cut(s) 1279
Cfr13I GGNCC 6 cut(s) 873, 1282, 1483, 1717, 1793, 2024
Cfr42I CCGCGG 1 cut(s) 1135
ClaI ATCGAT 1 cut(s) 1209
CseI GACGC 1 cut(s) 1912
CsiI ACCWGGT 1 cut(s) 1000
Csp6I GTAC 7 cut(s) 123, 189, 350, 868, 1122, 1129, 1580
CspCI CAANNNNNGTGG 2 cut(s) 375, 410
CviAII CATG 7 cut(s) 169, 361, 517, 1592, 2122, 2404, 2731
CviQI GTAC 7 cut(s) 123, 189, 350, 868, 1122, 1129, 1580
DdeI CTNAG 7 cut(s) 205, 450, 972, 1634, 1735, 2046, 2832
DraIII CACNNNGTG 1 cut(s) 1465
DrdI GACNNNNNNGTC 2 cut(s) 429, 1986
DseDI GACNNNNNNGTC 2 cut(s) 429, 1986
EaeI YGGCCR 5 cut(s) 243, 658, 1924, 1997, 2418
Eam1104I CTCTTC 7 cut(s) 328, 636, 891, 919, 1893, 1979, 2695
EarI CTCTTC 7 cut(s) 328, 636, 891, 919, 1893, 1979, 2695
EciI GGCGGA 1 cut(s) 982
Ecl136II GAGCTC 2 cut(s) 1970, 2050
Eco130I CCWWGG 4 cut(s) 296, 498, 516, 2421
Eco147I AGGCCT 1 cut(s) 2692
Eco24I GRGCYC 2 cut(s) 1972, 2052
Eco31I GGTCTC 1 cut(s) 473
Eco47I GGWCC 3 cut(s) 873, 1717, 2024
Eco47III AGCGCT 1 cut(s) 2830
Eco53kI GAGCTC 2 cut(s) 1970, 2050
Eco57I CTGAAG 3 cut(s) 1626, 2003, 2106
Eco72I CACGTG 1 cut(s) 944
EcoICRI GAGCTC 2 cut(s) 1970, 2050
EcoO109I RGGNCCY 1 cut(s) 1483
EcoRI GAATTC 2 cut(s) 543, 739
EcoRII CCWGG 3 cut(s) 1000, 1926, 2161
EcoT14I CCWWGG 4 cut(s) 296, 498, 516, 2421
EcoT38I GRGCYC 2 cut(s) 1972, 2052
ErhI CCWWGG 4 cut(s) 296, 498, 516, 2421
Esp3I CGTCTC 1 cut(s) 1895
FaeI CATG 7 cut(s) 172, 364, 520, 1595, 2125, 2407, 2734
FaqI GGGAC 2 cut(s) 708, 886
FatI CATG 7 cut(s) 168, 360, 516, 1591, 2121, 2403, 2730
FauI CCCGC 1 cut(s) 1908
FauNDI CATATG 1 cut(s) 783
FbaI TGATCA 1 cut(s) 820
FblI GTMKAC 1 cut(s) 1472
Fnu4HI GCNGC 8 cut(s) 256, 723, 1242, 1263, 1494, 1497, 1502, 2540
FriOI GRGCYC 2 cut(s) 1972, 2052
Fsp4HI GCNGC 8 cut(s) 256, 723, 1242, 1263, 1494, 1497, 1502, 2540
FspI TGCGCA 1 cut(s) 1500
GlaI GCGC 2 cut(s) 1500, 2830
GluI GCNGC 8 cut(s) 256, 723, 1242, 1263, 1494, 1497, 1502, 2540
GsaI CCCAGC 2 cut(s) 1361, 1695
GsuI CTGGAG 1 cut(s) 1047
HaeII RGCGCY 1 cut(s) 2832
HaeIII GGCC 9 cut(s) 245, 660, 1283, 1484, 1794, 1926, 1999, 2420, 2692
HapII CCGG 2 cut(s) 1280, 2417
HgaI GACGC 1 cut(s) 1912
HhaI GCGC 2 cut(s) 1501, 2831
Hin1I GRCGYC 1 cut(s) 2360
Hin1II CATG 7 cut(s) 172, 364, 520, 1595, 2125, 2407, 2734
Hin6I GCGC 2 cut(s) 1499, 2829
HinP1I GCGC 2 cut(s) 1499, 2829
HincII GTYRAC 1 cut(s) 1866
HindII GTYRAC 1 cut(s) 1866
HinfI GANTC 9 cut(s) 210, 416, 1374, 1453, 1874, 1987, 2090, 2771, 2792
HpaII CCGG 2 cut(s) 1280, 2417
HphI GGTGA 7 cut(s) 488, 1202, 1307, 1448, 2110, 2479, 2780
Hpy166II GTNNAC 7 cut(s) 123, 350, 375, 1219, 1298, 1473, 1866
Hpy188I TCNGA 9 cut(s) 307, 315, 507, 679, 1635, 1879, 2047, 2086, 2797
Hpy8I GTNNAC 7 cut(s) 123, 350, 375, 1219, 1298, 1473, 1866
Hpy99I CGWCG 2 cut(s) 1968, 2214
HpyCH4III ACNGT 5 cut(s) 1087, 1405, 1579, 2137, 2329
HpyCH4IV ACGT 3 cut(s) 943, 1042, 2360
HpyF10VI GCNNNNNNNGC 8 cut(s) 657, 1241, 1250, 1259, 1490, 1912, 1943, 2079
HpyF3I CTNAG 7 cut(s) 205, 450, 972, 1634, 1735, 2046, 2832
HpySE526I ACGT 3 cut(s) 943, 1042, 2360
Hsp92I GRCGYC 1 cut(s) 2360
Hsp92II CATG 7 cut(s) 172, 364, 520, 1595, 2125, 2407, 2734
HspAI GCGC 2 cut(s) 1499, 2829
KpnI GGTACC 1 cut(s) 1125
KroI GCCGGC 1 cut(s) 1279
KroNI GCCGGC 1 cut(s) 1281
Ksp22I TGATCA 1 cut(s) 820
KspI CCGCGG 1 cut(s) 1135
LguI GCTCTTC 2 cut(s) 328, 2695
LmnI GCTCC 9 cut(s) 143, 1066, 1232, 1238, 1250, 1313, 1590, 1911, 2684
Lsp1109I GCAGC 8 cut(s) 267, 734, 1249, 1253, 1483, 1505, 1513, 2551
LweI GCATC 4 cut(s) 1048, 1249, 2268, 2733
MabI ACCWGGT 1 cut(s) 1000
MaeII ACGT 3 cut(s) 943, 1042, 2360
MaeIII GTNAC 7 cut(s) 431, 1741, 1801, 2174, 2399, 2444, 2629
MbiI CCGCTC 1 cut(s) 370
MfeI CAATTG 4 cut(s) 1020, 1254, 1548, 2001
MflI RGATCY 5 cut(s) 456, 594, 674, 910, 1731
MhlI GDGCHC 6 cut(s) 148, 449, 1221, 1972, 2052, 2817
MlsI TGGCCA 3 cut(s) 245, 660, 1926
MluNI TGGCCA 3 cut(s) 245, 660, 1926
MlyI GAGTC 2 cut(s) 204, 1996
Mox20I TGGCCA 3 cut(s) 245, 660, 1926
MroNI GCCGGC 1 cut(s) 1279
MroXI GAANNNNTTC 3 cut(s) 571, 1197, 2205
MscI TGGCCA 3 cut(s) 245, 660, 1926
MslI CAYNNNNRTG 3 cut(s) 2228, 2262, 2354
Msp20I TGGCCA 3 cut(s) 245, 660, 1926
MspA1I CMGCKG 4 cut(s) 1134, 1496, 2194, 2542
MspCI CTTAAG 1 cut(s) 1721
MspI CCGG 2 cut(s) 1280, 2417
MspR9I CCNGG 4 cut(s) 1002, 1928, 2163, 2417
MunI CAATTG 4 cut(s) 1020, 1254, 1548, 2001
MvaI CCWGG 3 cut(s) 1002, 1928, 2163
MvnI CGCG 2 cut(s) 1134, 1704
MwoI GCNNNNNNNGC 8 cut(s) 657, 1241, 1250, 1259, 1490, 1912, 1943, 2079
NaeI GCCGGC 1 cut(s) 1281
NciI CCSGG 1 cut(s) 2417
NcoI CCATGG 1 cut(s) 516
NdeI CATATG 1 cut(s) 783
NgoMIV GCCGGC 1 cut(s) 1279
NheI GCTAGC 2 cut(s) 338, 889
NlaIII CATG 7 cut(s) 172, 364, 520, 1595, 2125, 2407, 2734
NlaIV GGNNCC 8 cut(s) 304, 676, 874, 912, 1123, 1315, 1586, 2155
NmuCI GTSAC 2 cut(s) 431, 2629
NsbI TGCGCA 1 cut(s) 1500
OliI CACNNNNGTG 1 cut(s) 2228
PceI AGGCCT 1 cut(s) 2692
PciSI GCTCTTC 2 cut(s) 328, 2695
PcsI WCGNNNNNNNCGW 2 cut(s) 616, 1969
PdiI GCCGGC 1 cut(s) 1281
PdmI GAANNNNTTC 3 cut(s) 571, 1197, 2205
PfeI GAWTC 7 cut(s) 416, 1374, 1453, 1874, 2090, 2771, 2792
PflFI GACNNNGTC 1 cut(s) 429
PflMI CCANNNNNTGG 3 cut(s) 787, 958, 2681
PkrI GCNGC 8 cut(s) 257, 724, 1243, 1264, 1495, 1498, 1503, 2541
Ple19I CGATCG 1 cut(s) 1528
PleI GAGTC 2 cut(s) 204, 1995
PmaCI CACGTG 1 cut(s) 944
PmlI CACGTG 1 cut(s) 944
PpsI GAGTC 2 cut(s) 204, 1995
Ppu21I YACGTR 1 cut(s) 944
PshBI ATTAAT 1 cut(s) 525
PsiI TTATAA 1 cut(s) 1091
Psp124BI GAGCTC 2 cut(s) 1972, 2052
Psp6I CCWGG 3 cut(s) 1000, 1926, 2161
PspCI CACGTG 1 cut(s) 944
PspFI CCCAGC 2 cut(s) 1357, 1691
PspGI CCWGG 3 cut(s) 1000, 1926, 2161
PspN4I GGNNCC 8 cut(s) 304, 676, 874, 912, 1123, 1315, 1586, 2155
PspPI GGNCC 6 cut(s) 873, 1282, 1483, 1717, 1793, 2024
PstI CTGCAG 1 cut(s) 1305
PstNI CAGNNNCTG 1 cut(s) 812
PsuI RGATCY 5 cut(s) 456, 594, 674, 910, 1731
PsyI GACNNNGTC 1 cut(s) 429
PvuI CGATCG 1 cut(s) 1528
PvuII CAGCTG 3 cut(s) 1496, 2194, 2542
RgaI GCGATCGC 1 cut(s) 1528
RsaI GTAC 7 cut(s) 124, 190, 351, 869, 1123, 1130, 1581
RsaNI GTAC 7 cut(s) 123, 189, 350, 868, 1122, 1129, 1580
RseI CAYNNNNRTG 3 cut(s) 2228, 2262, 2354
SacI GAGCTC 2 cut(s) 1972, 2052
SacII CCGCGG 1 cut(s) 1135
SapI GCTCTTC 2 cut(s) 328, 2695
SatI GCNGC 8 cut(s) 256, 723, 1242, 1263, 1494, 1497, 1502, 2540
Sau96I GGNCC 6 cut(s) 873, 1282, 1483, 1717, 1793, 2024
ScaI AGTACT 1 cut(s) 190
SchI GAGTC 2 cut(s) 204, 1996
ScrFI CCNGG 4 cut(s) 1002, 1928, 2163, 2417
SduI GDGCHC 6 cut(s) 148, 449, 1221, 1972, 2052, 2817
SexAI ACCWGGT 1 cut(s) 1000
SfaAI GCGATCGC 1 cut(s) 1528
SfaNI GCATC 4 cut(s) 1048, 1249, 2268, 2733
SfcI CTRYAG 2 cut(s) 1301, 1575
Sfr303I CCGCGG 1 cut(s) 1135
SgfI GCGATCGC 1 cut(s) 1528
SgrBI CCGCGG 1 cut(s) 1135
SinI GGWCC 3 cut(s) 873, 1717, 2024
SmiMI CAYNNNNRTG 3 cut(s) 2228, 2262, 2354
SmlI CTYRAG 5 cut(s) 1614, 1721, 1889, 2291, 2603
SmoI CTYRAG 5 cut(s) 1614, 1721, 1889, 2291, 2603
SpeI ACTAGT 1 cut(s) 185
SseBI AGGCCT 1 cut(s) 2692
SsiI CCGC 7 cut(s) 370, 967, 1132, 1134, 1915, 2187, 2397
SspI AATATT 3 cut(s) 37, 763, 1630
SstI GAGCTC 2 cut(s) 1972, 2052
StuI AGGCCT 1 cut(s) 2692
StyD4I CCNGG 4 cut(s) 1000, 1926, 2161, 2415
StyI CCWWGG 4 cut(s) 296, 498, 516, 2421
TaaI ACNGT 5 cut(s) 1087, 1405, 1579, 2137, 2329
TaiI ACGT 3 cut(s) 946, 1045, 2363
TaqI TCGA 8 cut(s) 639, 703, 750, 1209, 1438, 1560, 1966, 2494
TatI WGTACW 2 cut(s) 122, 188
TfiI GAWTC 7 cut(s) 416, 1374, 1453, 1874, 2090, 2771, 2792
TscAI CASTG 5 cut(s) 454, 799, 1310, 2140, 2383
TseFI GTSAC 2 cut(s) 431, 2629
TseI GCWGC 8 cut(s) 255, 722, 1241, 1262, 1493, 1496, 1501, 2539
Tsp45I GTSAC 2 cut(s) 431, 2629
TspGWI ACGGA 2 cut(s) 203, 1577
TspRI CASTG 5 cut(s) 454, 799, 1310, 2140, 2383
Tth111I GACNNNGTC 1 cut(s) 429
Van91I CCANNNNNTGG 3 cut(s) 787, 958, 2681
Vha464I CTTAAG 1 cut(s) 1721
VneI GTGCAC 1 cut(s) 1217
VpaK11BI GGWCC 3 cut(s) 873, 1717, 2024
VspI ATTAAT 1 cut(s) 525
XapI RAATTY 4 cut(s) 543, 739, 954, 1267
XcmI CCANNNNNNNNNTGG 2 cut(s) 622, 668
XmiI GTMKAC 1 cut(s) 1472
XmnI GAANNNNTTC 3 cut(s) 571, 1197, 2205
ZraI GACGTC 1 cut(s) 2361
ZrmI AGTACT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.