Rroxscaffold_3G00234850

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008218
Physical Location & Seq
Forward (+)
21539578 .. 21541705
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00234850.1

Sequence Viewer

Length: 1578 bp
ATGCACAATTTAATGGGAGTAGGAGCAGCAGAAGGAGGAATTGATGCTTCAAATTTATTGAAGCCGGCTCTTGCAAGAGGTAAACTGCAGTGCATTGGAGCCACAACAATAGATGAATACAGAAAGCATGTTGAAAAAGACCCAGCATTCGAAAGAAGATTCCAACCTGTCAAAGTTCCTATGCCAACTGTGGATAAAGCCATACAGATTTTGAGAGGGCTTCAAGAACGATATGAGATCCACCACAAAGTCCAGTATACTGATGAGGCCCTGAAAGCAGCTGCGCAGCTATCTCACCGATCCATCAGCGATCGCTTTCTTCCTGATAAAGCAATTGACTTGATCGATGAAGCTAGTTCTGCAGTAAGGCTGCGTCCTACACAGGTTCCTAAAGAAGCTCAAGAGCTTGACAATATTCTGAGGCAAATAACAAAAGAGAAGAATGAAGCTATTCATAGCCATGAGTTTGAGAAGATACATAGTTTTTGTTGCTGGAATTCTGCTGCGCAATTAGGTGACAGAGAATTGGACCTTAAGACACAGATCTCAGAAGTAATAGAGAAAGGCAGGGAGATAAGTGAGGCATTGGATTATATTAATGGTGGCCGTATTGTTACTGAATTGGATATTCAACAAGTTGTGTCCTGTATGACTCATATTCCACTTGAGGAAGTGTCAACAAATGAACCCAATCGCCTCCTCAAGATGGAAGAGACCCTCTATAAGCGGGTTATTGGTCAGGATGAAGCAGTCAGCGCTATTAGCAAAGCTATTCGTCGAGCTCGTGTTGGACTGAGGAATCCTAATCGGCCAATTGCCAGTTTCATCTTTTGTGGTCCAACAGGTGTTGGAAAATCTGAGCTCGTAAAAACTTTGGCGACTAGCTATTTTGGCTCTGAAGAATCAATAGTAAGGCTCTATATGAGTGAATTCATGGAAGAGCACTCTGTTTCCAAGCTCATTGGTTCTCCCCTTGGTTATGTTGGTTACACAGAAGGTGGTCAGCTGGTAGAAGCCGTTCGTCGCCGTCCTTACACTCTGGTGCTTTTTGATGAGATTGAGAAGGCTCATCCTAAAATTTTCAACTTGATGCTTCAAATACTTGAGGATGGAAGATTGACTGACAGCAAAGGCAGAACTGTAGATTTCAAGAATACACTAGTTATTATGACGTCTAACAGTGAGATTGAGAGAGGAGGCCGCAAAATGGGATTTGACCTGGCCATGGATGAAGACAAGGATGGCAGTTACAAAAAAATTAAGGGCTTCGTGACAGAGAAATTGAAACACCACTTTCGACCAGAGTTCTTAAATAGGTTGGATGAAATCATTGTTTTTCGGCAGCTGACTAAGTTGGACGTAAAGGAAATTGCAAATATAATGCTGAACGAGTATTATGGAATGCTCAAGTCCAAAGAGATAGAGCTTAGGGTGACCAAGAGGTTCAAAGACAAAGTGGTTGACGAAGGATACAACCCAACCTATGGAGCAAGGCCTTTAAGAAGAGTCATAATGAGACTTTTGGTGGACACCATGGCTGAGAAGATGCTGGCAAAGGAGATCAAAGAGGGCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004176 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006605 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0008104 GO:0008150 GO:0008152 GO:0008233 GO:0009507 GO:0009526 GO:0009528 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009657 GO:0009658 GO:0009706 GO:0009719 GO:0009725 GO:0009735 GO:0009889 GO:0009941 GO:0009987 GO:0010033 GO:0010380 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017038 GO:0017111 GO:0019222 GO:0019538 GO:0019866 GO:0022607 GO:0030312 GO:0031090 GO:0031323 GO:0031326 GO:0031897 GO:0031967 GO:0031969 GO:0031975 GO:0031976 GO:0031984 GO:0032991 GO:0033036 GO:0033365 GO:0034214 GO:0034357 GO:0034613 GO:0042170 GO:0042221 GO:0042623 GO:0042651 GO:0042802 GO:0042803 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0046983 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051179 GO:0051193 GO:0051234 GO:0051259 GO:0051641 GO:0051649 GO:0055035 GO:0055085 GO:0065002 GO:0065003 GO:0065007 GO:0070011 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0071944 GO:0072594 GO:0072596 GO:0072598 GO:0090056 GO:0098796 GO:0140096 GO:1901401 GO:1901463 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko01100 map01100
Pfam Domains
Protein Families
AAA AAA_2 ClpB_D2-small Clp_N UVR

Protein Analysis

525

Amino Acids

59.74

Weight (kDa)

7.23

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_lid_9 PF17871 64 - 158 6.2e-32 AAA lid domain
AAA_2 PF07724 270 - 443 5e-60 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 275 - 400 2e-11 AAA domain (dynein-related subfamily)
AAA PF00004 276 - 394 9.5e-06 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 449 - 525 2.8e-21 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1175
Acc16I TGCGCA 2 cut(s) 285, 507
AccB7I CCANNNNNTGG 1 cut(s) 1484
AccI GTMKAC 1 cut(s) 257
AciI CCGC 2 cut(s) 727, 1201
AclWI GGATC 2 cut(s) 232, 294
AcoI YGGCCR 3 cut(s) 604, 809, 1221
AcsI RAATTY 4 cut(s) 52, 496, 929, 1077
AcuI CTGAAG 1 cut(s) 918
AcyI GRCGYC 1 cut(s) 1172
AfeI AGCGCT 1 cut(s) 757
AfiI CCNNNNNNNGG 4 cut(s) 706, 1207, 1225, 1484
AflII CTTAAG 1 cut(s) 533
AhlI ACTAGT 1 cut(s) 1159
AjnI CCWGG 1 cut(s) 1218
AleI CACNNNNGTG 1 cut(s) 1040
Alw21I GWGCWC 3 cut(s) 784, 864, 945
Alw26I GTCTC 2 cut(s) 707, 1510
AlwI GGATC 2 cut(s) 232, 294
Aor51HI AGCGCT 1 cut(s) 757
AoxI GGCC 6 cut(s) 267, 604, 809, 1198, 1221, 1493
ApeKI GCWGC 7 cut(s) 26, 278, 281, 286, 370, 503, 1342
ApoI RAATTY 4 cut(s) 52, 496, 929, 1077
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AseI ATTAAT 1 cut(s) 597
AsiSI GCGATCGC 1 cut(s) 313
Asp700I GAANNNNTTC 2 cut(s) 450, 1017
AspLEI GCGC 3 cut(s) 286, 508, 758
AspS9I GGNCC 3 cut(s) 268, 529, 836
AsuHPI GGTGA 3 cut(s) 287, 527, 1444
AsuII TTCGAA 1 cut(s) 150
AvaII GGWCC 2 cut(s) 529, 836
BalI TGGCCA 1 cut(s) 1223
BanII GRGCYC 2 cut(s) 784, 864
BauI CACGAG 1 cut(s) 783
BbsI GAAGAC 1 cut(s) 1239
Bbv12I GWGCWC 3 cut(s) 784, 864, 945
BbvI GCAGC 7 cut(s) 38, 268, 290, 298, 357, 490, 1354
BccI CCATC 4 cut(s) 311, 700, 1103, 1235
BceAI ACGGC 3 cut(s) 591, 1001, 1011
BciT130I CCWGG 1 cut(s) 1220
BciVI GTATCC 1 cut(s) 1463
BcoDI GTCTC 2 cut(s) 707, 1510
BcuI ACTAGT 1 cut(s) 1159
BfaI CTAG 3 cut(s) 354, 882, 1160
BfmI CTRYAG 3 cut(s) 86, 360, 1140
BfoI RGCGCY 1 cut(s) 759
BfrI CTTAAG 1 cut(s) 533
BfuI GTATCC 1 cut(s) 1463
BglII AGATCT 1 cut(s) 543
BisI GCNGC 8 cut(s) 27, 279, 282, 287, 371, 504, 1201, 1343
BlsI GCNGC 8 cut(s) 28, 280, 283, 288, 372, 505, 1202, 1344
Bme1390I CCNGG 1 cut(s) 1220
Bme18I GGWCC 2 cut(s) 529, 836
BmgT120I GGNCC 3 cut(s) 268, 529, 836
BmiI GGNNCC 2 cut(s) 100, 387
BmrFI CCNGG 1 cut(s) 1220
BmsI GCATC 3 cut(s) 34, 1080, 1536
BpiI GAAGAC 1 cut(s) 1239
Bpu10I CCTNAGC 1 cut(s) 1427
Bpu14I TTCGAA 1 cut(s) 150
BpuEI CTTGAG 5 cut(s) 384, 686, 686, 1124, 1391
Bsa29I ATCGAT 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 1172
BsaI GGTCTC 1 cut(s) 707
BsaJI CCNNGG 3 cut(s) 973, 1224, 1533
Bsc4I CCNNNNNNNGG 4 cut(s) 706, 1207, 1225, 1484
Bse118I RCCGGY 1 cut(s) 64
Bse1I ACTGG 2 cut(s) 253, 819
BseBI CCWGG 1 cut(s) 1220
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 3 cut(s) 973, 1224, 1533
BseGI GGATG 6 cut(s) 748, 1069, 1114, 1234, 1246, 1327
BseLI CCNNNNNNNGG 4 cut(s) 706, 1207, 1225, 1484
BseMII CTCAG 5 cut(s) 410, 561, 785, 849, 1530
BseNI ACTGG 2 cut(s) 253, 819
BseRI GAGGAG 2 cut(s) 689, 1209
BseXI GCAGC 7 cut(s) 38, 268, 290, 298, 357, 490, 1354
BseYI CCCAGC 1 cut(s) 142
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 6 cut(s) 269, 606, 811, 1200, 1223, 1495
BshVI ATCGAT 1 cut(s) 345
BsiEI CGRYCG 1 cut(s) 313
BsiHKAI GWGCWC 3 cut(s) 784, 864, 945
BsiSI CCGG 1 cut(s) 65
BslI CCNNNNNNNGG 4 cut(s) 706, 1207, 1225, 1484
BsmAI GTCTC 2 cut(s) 707, 1510
BsmI GAATGC 2 cut(s) 146, 1407
BsnI GGCC 6 cut(s) 269, 606, 811, 1200, 1223, 1495
Bso31I GGTCTC 1 cut(s) 707
Bsp119I TTCGAA 1 cut(s) 150
Bsp1286I GDGCHC 3 cut(s) 784, 864, 945
Bsp143I GATC 6 cut(s) 237, 299, 310, 342, 543, 1560
Bsp19I CCATGG 2 cut(s) 1224, 1533
BspACI CCGC 2 cut(s) 727, 1201
BspANI GGCC 6 cut(s) 269, 606, 811, 1200, 1223, 1495
BspCNI CTCAG 5 cut(s) 411, 560, 786, 850, 1531
BspDI ATCGAT 1 cut(s) 345
BspLI GGNNCC 2 cut(s) 100, 387
BspMAI CTGCAG 2 cut(s) 90, 364
BspPI GGATC 2 cut(s) 232, 294
BspQI GCTCTTC 1 cut(s) 933
BspT104I TTCGAA 1 cut(s) 150
BspTI CTTAAG 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 707
BsrFI RCCGGY 1 cut(s) 64
BsrI ACTGG 2 cut(s) 253, 819
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 3 cut(s) 973, 1224, 1533
BssMI GATC 6 cut(s) 237, 299, 310, 342, 543, 1560
BssNAI GTATAC 1 cut(s) 258
BssNI GRCGYC 1 cut(s) 1172
BssSI CACGAG 1 cut(s) 783
BssT1I CCWWGG 3 cut(s) 973, 1224, 1533
Bst1107I GTATAC 1 cut(s) 258
Bst2BI CACGAG 1 cut(s) 783
Bst2UI CCWGG 1 cut(s) 1220
Bst4CI ACNGT 3 cut(s) 190, 1141, 1181
Bst6I CTCTTC 3 cut(s) 705, 933, 1498
BstACI GRCGYC 1 cut(s) 1172
BstAFI CTTAAG 1 cut(s) 533
BstBI TTCGAA 1 cut(s) 150
BstC8I GCNNGC 2 cut(s) 66, 1551
BstDEI CTNAG 7 cut(s) 419, 547, 794, 858, 1350, 1427, 1539
BstDSI CCRYGG 2 cut(s) 1224, 1533
BstEII GGTNACC 1 cut(s) 1432
BstF5I GGATG 6 cut(s) 748, 1069, 1114, 1234, 1246, 1327
BstH2I RGCGCY 1 cut(s) 759
BstHHI GCGC 3 cut(s) 286, 508, 758
BstKTI GATC 6 cut(s) 240, 302, 313, 345, 546, 1563
BstMAI GTCTC 2 cut(s) 707, 1510
BstMBI GATC 6 cut(s) 237, 299, 310, 342, 543, 1560
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 5 cut(s) 275, 359, 755, 762, 891
BstNI CCWGG 1 cut(s) 1220
BstNSI RCATGY 1 cut(s) 131
BstPI GGTNACC 1 cut(s) 1432
BstSCI CCNGG 1 cut(s) 1218
BstSFI CTRYAG 3 cut(s) 86, 360, 1140
BstV1I GCAGC 7 cut(s) 38, 268, 290, 298, 357, 490, 1354
BstV2I GAAGAC 1 cut(s) 1239
BstX2I RGATCY 2 cut(s) 237, 543
BstYI RGATCY 2 cut(s) 237, 543
BstZ17I GTATAC 1 cut(s) 258
Bsu15I ATCGAT 1 cut(s) 345
BsuI GTATCC 1 cut(s) 1463
BsuRI GGCC 6 cut(s) 269, 606, 811, 1200, 1223, 1495
BsuTUI ATCGAT 1 cut(s) 345
BtgI CCRYGG 2 cut(s) 1224, 1533
BtsCI GGATG 6 cut(s) 748, 1069, 1114, 1234, 1246, 1327
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 2 cut(s) 95, 1186
Cac8I GCNNGC 2 cut(s) 66, 1551
CfoI GCGC 3 cut(s) 286, 508, 758
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 3 cut(s) 268, 529, 836
ClaI ATCGAT 1 cut(s) 345
CseI GACGC 1 cut(s) 362
CviAII CATG 5 cut(s) 128, 461, 934, 1225, 1534
DdeI CTNAG 7 cut(s) 419, 547, 794, 858, 1350, 1427, 1539
DpnI GATC 6 cut(s) 239, 301, 312, 344, 545, 1562
DpnII GATC 6 cut(s) 237, 299, 310, 342, 543, 1560
EaeI YGGCCR 3 cut(s) 604, 809, 1221
Eam1104I CTCTTC 3 cut(s) 705, 933, 1498
EarI CTCTTC 3 cut(s) 705, 933, 1498
Ecl136II GAGCTC 2 cut(s) 782, 862
Eco130I CCWWGG 3 cut(s) 973, 1224, 1533
Eco147I AGGCCT 1 cut(s) 1495
Eco24I GRGCYC 2 cut(s) 784, 864
Eco31I GGTCTC 1 cut(s) 707
Eco47I GGWCC 2 cut(s) 529, 836
Eco47III AGCGCT 1 cut(s) 757
Eco53kI GAGCTC 2 cut(s) 782, 862
Eco57I CTGAAG 1 cut(s) 918
Eco91I GGTNACC 1 cut(s) 1432
EcoICRI GAGCTC 2 cut(s) 782, 862
EcoO109I RGGNCCY 1 cut(s) 268
EcoO65I GGTNACC 1 cut(s) 1432
EcoRI GAATTC 2 cut(s) 496, 929
EcoRII CCWGG 1 cut(s) 1218
EcoT14I CCWWGG 3 cut(s) 973, 1224, 1533
EcoT38I GRGCYC 2 cut(s) 784, 864
ErhI CCWWGG 3 cut(s) 973, 1224, 1533
FaeI CATG 5 cut(s) 131, 464, 937, 1228, 1537
FatI CATG 5 cut(s) 127, 460, 933, 1224, 1533
FauI CCCGC 1 cut(s) 720
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 8 cut(s) 27, 279, 282, 287, 371, 504, 1201, 1343
FokI GGATG 6 cut(s) 755, 1056, 1121, 1241, 1253, 1334
FriOI GRGCYC 2 cut(s) 784, 864
Fsp4HI GCNGC 8 cut(s) 27, 279, 282, 287, 371, 504, 1201, 1343
FspBI CTAG 3 cut(s) 354, 882, 1160
FspI TGCGCA 2 cut(s) 285, 507
GlaI GCGC 3 cut(s) 285, 507, 757
GluI GCNGC 8 cut(s) 27, 279, 282, 287, 371, 504, 1201, 1343
GsaI CCCAGC 1 cut(s) 146
HaeII RGCGCY 1 cut(s) 759
HaeIII GGCC 6 cut(s) 269, 606, 811, 1200, 1223, 1495
HapII CCGG 1 cut(s) 65
HgaI GACGC 1 cut(s) 362
HhaI GCGC 3 cut(s) 286, 508, 758
Hin1I GRCGYC 1 cut(s) 1172
Hin1II CATG 5 cut(s) 131, 464, 937, 1228, 1537
Hin6I GCGC 3 cut(s) 284, 506, 756
HinP1I GCGC 3 cut(s) 284, 506, 756
HincII GTYRAC 2 cut(s) 678, 1462
HindII GTYRAC 2 cut(s) 678, 1462
HinfI GANTC 5 cut(s) 159, 652, 799, 902, 1506
HpaII CCGG 1 cut(s) 65
HphI GGTGA 3 cut(s) 287, 527, 1444
Hpy166II GTNNAC 5 cut(s) 83, 258, 678, 1462, 1528
Hpy188I TCNGA 4 cut(s) 420, 550, 859, 898
Hpy188III TCNNGA 7 cut(s) 224, 323, 401, 703, 740, 1150, 1270
Hpy8I GTNNAC 5 cut(s) 83, 258, 678, 1462, 1528
Hpy99I CGWCG 2 cut(s) 780, 1026
HpyAV CCTTC 4 cut(s) 26, 989, 1057, 1460
HpyCH4III ACNGT 3 cut(s) 190, 1141, 1181
HpyCH4IV ACGT 2 cut(s) 1172, 1359
HpyCH4V TGCA 6 cut(s) 4, 74, 88, 93, 362, 1373
HpyF10VI GCNNNNNNNGC 5 cut(s) 275, 359, 755, 762, 891
HpyF3I CTNAG 7 cut(s) 419, 547, 794, 858, 1350, 1427, 1539
HpySE526I ACGT 2 cut(s) 1172, 1359
Hsp92I GRCGYC 1 cut(s) 1172
Hsp92II CATG 5 cut(s) 131, 464, 937, 1228, 1537
HspAI GCGC 3 cut(s) 284, 506, 756
KroI GCCGGC 1 cut(s) 64
KroNI GCCGGC 1 cut(s) 66
Kzo9I GATC 6 cut(s) 237, 299, 310, 342, 543, 1560
LguI GCTCTTC 1 cut(s) 933
LmnI GCTCC 3 cut(s) 23, 98, 1487
Lsp1109I GCAGC 7 cut(s) 38, 268, 290, 298, 357, 490, 1354
LweI GCATC 3 cut(s) 34, 1080, 1536
MaeI CTAG 3 cut(s) 354, 882, 1160
MaeII ACGT 2 cut(s) 1172, 1359
MaeIII GTNAC 6 cut(s) 515, 613, 986, 1247, 1270, 1432
MalI GATC 6 cut(s) 239, 301, 312, 344, 545, 1562
MboI GATC 6 cut(s) 237, 299, 310, 342, 543, 1560
MfeI CAATTG 2 cut(s) 333, 813
MflI RGATCY 2 cut(s) 237, 543
MhlI GDGCHC 3 cut(s) 784, 864, 945
MlsI TGGCCA 1 cut(s) 1223
MluNI TGGCCA 1 cut(s) 1223
MlyI GAGTC 2 cut(s) 646, 1515
MmeI TCCRAC 6 cut(s) 187, 769, 829, 863, 1299, 1335
Mox20I TGGCCA 1 cut(s) 1223
MroNI GCCGGC 1 cut(s) 64
MroXI GAANNNNTTC 2 cut(s) 450, 1017
MscI TGGCCA 1 cut(s) 1223
MseI TTAA 6 cut(s) 11, 534, 597, 1260, 1310, 1499
MslI CAYNNNNRTG 2 cut(s) 459, 1040
Msp20I TGGCCA 1 cut(s) 1223
MspA1I CMGCKG 3 cut(s) 281, 1006, 1345
MspCI CTTAAG 1 cut(s) 533
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 1 cut(s) 1220
MunI CAATTG 2 cut(s) 333, 813
Mva1269I GAATGC 2 cut(s) 146, 1407
MvaI CCWGG 1 cut(s) 1220
MwoI GCNNNNNNNGC 5 cut(s) 275, 359, 755, 762, 891
NaeI GCCGGC 1 cut(s) 66
NcoI CCATGG 2 cut(s) 1224, 1533
NdeII GATC 6 cut(s) 237, 299, 310, 342, 543, 1560
NgoMIV GCCGGC 1 cut(s) 64
NlaIII CATG 5 cut(s) 131, 464, 937, 1228, 1537
NlaIV GGNNCC 2 cut(s) 100, 387
NmuCI GTSAC 3 cut(s) 515, 1270, 1432
NsbI TGCGCA 2 cut(s) 285, 507
NspI RCATGY 1 cut(s) 131
NspV TTCGAA 1 cut(s) 150
OliI CACNNNNGTG 1 cut(s) 1040
PceI AGGCCT 1 cut(s) 1495
PciSI GCTCTTC 1 cut(s) 933
PcsI WCGNNNNNNNCGW 1 cut(s) 781
PctI GAATGC 2 cut(s) 146, 1407
PdiI GCCGGC 1 cut(s) 66
PdmI GAANNNNTTC 2 cut(s) 450, 1017
PfeI GAWTC 3 cut(s) 159, 799, 902
PflMI CCANNNNNTGG 1 cut(s) 1484
PkrI GCNGC 8 cut(s) 28, 280, 283, 288, 372, 505, 1202, 1344
Ple19I CGATCG 1 cut(s) 313
PleI GAGTC 2 cut(s) 646, 1514
PpsI GAGTC 2 cut(s) 646, 1514
PshBI ATTAAT 1 cut(s) 597
Psp124BI GAGCTC 2 cut(s) 784, 864
Psp6I CCWGG 1 cut(s) 1218
PspEI GGTNACC 1 cut(s) 1432
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 1 cut(s) 1218
PspN4I GGNNCC 2 cut(s) 100, 387
PspPI GGNCC 3 cut(s) 268, 529, 836
PsrI GAACNNNNNNTAC 2 cut(s) 1002, 1034
PstI CTGCAG 2 cut(s) 90, 364
PsuI RGATCY 2 cut(s) 237, 543
PvuI CGATCG 1 cut(s) 313
PvuII CAGCTG 3 cut(s) 281, 1006, 1345
RgaI GCGATCGC 1 cut(s) 313
RseI CAYNNNNRTG 2 cut(s) 459, 1040
SacI GAGCTC 2 cut(s) 784, 864
SapI GCTCTTC 1 cut(s) 933
SaqAI TTAA 6 cut(s) 11, 534, 597, 1260, 1310, 1499
SatI GCNGC 8 cut(s) 27, 279, 282, 287, 371, 504, 1201, 1343
Sau3AI GATC 6 cut(s) 237, 299, 310, 342, 543, 1560
Sau96I GGNCC 3 cut(s) 268, 529, 836
SchI GAGTC 2 cut(s) 646, 1515
ScrFI CCNGG 1 cut(s) 1220
SduI GDGCHC 3 cut(s) 784, 864, 945
SfaAI GCGATCGC 1 cut(s) 313
SfaNI GCATC 3 cut(s) 34, 1080, 1536
SfcI CTRYAG 3 cut(s) 86, 360, 1140
SfuI TTCGAA 1 cut(s) 150
SgfI GCGATCGC 1 cut(s) 313
SinI GGWCC 2 cut(s) 529, 836
SmiMI CAYNNNNRTG 2 cut(s) 459, 1040
SmlI CTYRAG 6 cut(s) 399, 533, 665, 701, 1103, 1406
SmoI CTYRAG 6 cut(s) 399, 533, 665, 701, 1103, 1406
SpeI ACTAGT 1 cut(s) 1159
SseBI AGGCCT 1 cut(s) 1495
SsiI CCGC 2 cut(s) 727, 1201
SspI AATATT 1 cut(s) 415
SspMI CTAG 3 cut(s) 354, 882, 1160
SstI GAGCTC 2 cut(s) 784, 864
StuI AGGCCT 1 cut(s) 1495
StyD4I CCNGG 1 cut(s) 1218
StyI CCWWGG 3 cut(s) 973, 1224, 1533
TaaI ACNGT 3 cut(s) 190, 1141, 1181
TaiI ACGT 2 cut(s) 1175, 1362
TaqI TCGA 4 cut(s) 150, 345, 778, 1297
TauI GCSGC 1 cut(s) 1203
TfiI GAWTC 3 cut(s) 159, 799, 902
Tru1I TTAA 6 cut(s) 11, 534, 597, 1260, 1310, 1499
Tru9I TTAA 6 cut(s) 11, 534, 597, 1260, 1310, 1499
TscAI CASTG 2 cut(s) 95, 1186
TseFI GTSAC 3 cut(s) 515, 1270, 1432
TseI GCWGC 7 cut(s) 26, 278, 281, 286, 370, 503, 1342
Tsp45I GTSAC 3 cut(s) 515, 1270, 1432
TspRI CASTG 2 cut(s) 95, 1186
Van91I CCANNNNNTGG 1 cut(s) 1484
Vha464I CTTAAG 1 cut(s) 533
VpaK11BI GGWCC 2 cut(s) 529, 836
VspI ATTAAT 1 cut(s) 597
XapI RAATTY 4 cut(s) 52, 496, 929, 1077
XceI RCATGY 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 257
XmnI GAANNNNTTC 2 cut(s) 450, 1017
XspI CTAG 3 cut(s) 354, 882, 1160
ZraI GACGTC 1 cut(s) 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.