RLG00000001903

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008218
Physical Location & Seq
Forward (+)
22796582 .. 22797355
774 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001903

Sequence Viewer

Length: 774 bp
ATGGCTACGGCCGTTTTGACTCTACCTTCAACAAACACTGGTATGGTTGGTGGGCTAGTTAGGGGTGGCCAATATAAAGAATCAGGTACAAGAAAGAGTAGTAGTCTGAACATGATCCCTGCTGCAGGGGGAGCAGCAGCTCTTGCTTTGAGAATGAGGTGCAAGAGTACTAGTAGCACTAGTAGTTTTTCTAGTTTCTCCGGACTCCATAGGTTTAATGCCCTAGATATGGTGGCCAGACCTAGCAGCCTAGGTGACGAGGACTACTATTCTGGAACAGCAATCCCTTGGGGTTCCGAAAGTCAAAAGGGGGCTAGAATGAGTGGAAGATCTAGCAGATGTGTACCTAAATCCATGTTTGAGCGGTTCACAGCCTTTTTCCCTTGGGGTTCCGAAAGTCAAAATGGGAATACAAGTGAAAGAGAGGATGATGAAACTAAAATTCAAAATGGGAATACAAGTGGAAGAGGGCATGATGAGACTAAAACGCCAACTCTAGAGGAGTGTGGGACTAATTTAACGAAGCTAGCAGAAGAGGGCAAGCTGGATCCTGTTGTTGGAAGAGAACAACAAATATCGCGTGTCATCCAAATTTTGAGTAAGAGGACTAAGAATAATGCATGCATTATTGGAGAACCTGGTGTTGGGAAGACTGCAATTGCAGAAGGTCTTGCTCAACGTATTGCAAGTGGCGATGTTCCAAAGTCAATAGCCAACAAGAAGGTTATAAGTCTTGATATGGCTAAACTTGTCTCTGGTACCAAGTACCACTGA

Protein Analysis

258

Amino Acids

27.35

Weight (kDa)

9.39

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_16 PF13191 184 - 229 1.7e-06 AAA ATPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 728
Acc65I GGTACC 1 cut(s) 758
AccB1I GGYRCC 1 cut(s) 758
AccBSI CCGCTC 1 cut(s) 364
AccII CGCG 1 cut(s) 580
AccIII TCCGGA 1 cut(s) 200
AciI CCGC 1 cut(s) 364
AclWI GGATC 3 cut(s) 109, 542, 555
AcoI YGGCCR 3 cut(s) 9, 67, 234
AcsI RAATTY 2 cut(s) 441, 591
AfaI GTAC 5 cut(s) 88, 169, 345, 760, 767
AfiI CCNNNNNNNGG 4 cut(s) 125, 229, 557, 644
AgsI TTSAA 2 cut(s) 30, 446
AhlI ACTAGT 2 cut(s) 170, 179
AjnI CCWGG 1 cut(s) 637
AjuI GAANNNNNNNTTGG 2 cut(s) 627, 659
AluBI AGCT 3 cut(s) 140, 526, 544
AluI AGCT 3 cut(s) 140, 526, 544
Alw26I GTCTC 2 cut(s) 473, 757
AlwI GGATC 3 cut(s) 109, 542, 555
Aor13HI TCCGGA 1 cut(s) 200
AoxI GGCC 3 cut(s) 9, 67, 234
ApeKI GCWGC 4 cut(s) 122, 134, 137, 246
ApoI RAATTY 2 cut(s) 441, 591
Asp718I GGTACC 1 cut(s) 758
AspA2I CCTAGG 1 cut(s) 250
AsuHPI GGTGA 1 cut(s) 266
AsuNHI GCTAGC 1 cut(s) 526
AvrII CCTAGG 1 cut(s) 250
BalI TGGCCA 2 cut(s) 69, 236
BamHI GGATCC 1 cut(s) 547
BanI GGYRCC 1 cut(s) 758
BbsI GAAGAC 1 cut(s) 656
BbvI GCAGC 4 cut(s) 109, 146, 149, 258
BceAI ACGGC 1 cut(s) 24
BciT130I CCWGG 1 cut(s) 639
BcoDI GTCTC 2 cut(s) 473, 757
BcuI ACTAGT 2 cut(s) 170, 179
BfmI CTRYAG 1 cut(s) 123
BglII AGATCT 1 cut(s) 329
BisI GCNGC 4 cut(s) 123, 135, 138, 247
BlnI CCTAGG 1 cut(s) 250
BlsI GCNGC 4 cut(s) 124, 136, 139, 248
BmcAI AGTACT 1 cut(s) 169
Bme1390I CCNGG 1 cut(s) 639
BmiI GGNNCC 4 cut(s) 295, 391, 549, 760
BmrFI CCNGG 1 cut(s) 639
BmtI GCTAGC 1 cut(s) 530
BpiI GAAGAC 1 cut(s) 656
BsaJI CCNNGG 3 cut(s) 250, 287, 383
BsaWI WCCGGW 1 cut(s) 200
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 229, 557, 644
Bse1I ACTGG 1 cut(s) 43
BseAI TCCGGA 1 cut(s) 200
BseBI CCWGG 1 cut(s) 639
BseDI CCNNGG 3 cut(s) 250, 287, 383
BseGI GGATG 2 cut(s) 433, 585
BseLI CCNNNNNNNGG 4 cut(s) 125, 229, 557, 644
BseNI ACTGG 1 cut(s) 43
BseRI GAGGAG 1 cut(s) 515
BseX3I CGGCCG 1 cut(s) 9
BseXI GCAGC 4 cut(s) 109, 146, 149, 258
Bsh1236I CGCG 1 cut(s) 580
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 3 cut(s) 11, 69, 236
BshNI GGYRCC 1 cut(s) 758
BsiEI CGRYCG 1 cut(s) 12
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 523
BslI CCNNNNNNNGG 4 cut(s) 125, 229, 557, 644
BsmAI GTCTC 2 cut(s) 473, 757
BsmFI GGGAC 1 cut(s) 523
BsnI GGCC 3 cut(s) 11, 69, 236
Bsp13I TCCGGA 1 cut(s) 200
Bsp143I GATC 3 cut(s) 114, 329, 547
BspACI CCGC 1 cut(s) 364
BspANI GGCC 3 cut(s) 11, 69, 236
BspEI TCCGGA 1 cut(s) 200
BspFNI CGCG 1 cut(s) 580
BspLI GGNNCC 4 cut(s) 295, 391, 549, 760
BspMAI CTGCAG 1 cut(s) 127
BspOI GCTAGC 1 cut(s) 530
BspPI GGATC 3 cut(s) 109, 542, 555
BspT107I GGYRCC 1 cut(s) 758
BsrBI CCGCTC 1 cut(s) 364
BsrI ACTGG 1 cut(s) 43
BssECI CCNNGG 3 cut(s) 250, 287, 383
BssMI GATC 3 cut(s) 114, 329, 547
BssT1I CCWWGG 3 cut(s) 250, 287, 383
Bst2UI CCWGG 1 cut(s) 639
Bst6I CTCTTC 3 cut(s) 460, 528, 556
BstAPI GCANNNNNTGC 1 cut(s) 143
BstC8I GCNNGC 3 cut(s) 528, 542, 622
BstDEI CTNAG 1 cut(s) 609
BstENI CCTNNNNNAGG 1 cut(s) 123
BstF5I GGATG 2 cut(s) 433, 585
BstFNI CGCG 1 cut(s) 580
BstKTI GATC 3 cut(s) 117, 332, 550
BstMAI GTCTC 2 cut(s) 473, 757
BstMBI GATC 3 cut(s) 114, 329, 547
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 2 cut(s) 131, 143
BstNI CCWGG 1 cut(s) 639
BstNSI RCATGY 1 cut(s) 624
BstSCI CCNGG 1 cut(s) 637
BstSFI CTRYAG 1 cut(s) 123
BstUI CGCG 1 cut(s) 580
BstV1I GCAGC 4 cut(s) 109, 146, 149, 258
BstV2I GAAGAC 1 cut(s) 656
BstX2I RGATCY 2 cut(s) 329, 547
BstYI RGATCY 2 cut(s) 329, 547
BstZI CGGCCG 1 cut(s) 9
BsuRI GGCC 3 cut(s) 11, 69, 236
BtgZI GCGATG 1 cut(s) 708
BtsCI GGATG 2 cut(s) 433, 585
BtsIMutI CAGTG 2 cut(s) 36, 769
Cac8I GCNNGC 3 cut(s) 528, 542, 622
CsiI ACCWGGT 1 cut(s) 637
Csp6I GTAC 5 cut(s) 87, 168, 344, 759, 766
CviAII CATG 4 cut(s) 112, 355, 473, 621
CviQI GTAC 5 cut(s) 87, 168, 344, 759, 766
DdeI CTNAG 1 cut(s) 609
DpnI GATC 3 cut(s) 116, 331, 549
DpnII GATC 3 cut(s) 114, 329, 547
EaeI YGGCCR 3 cut(s) 9, 67, 234
EagI CGGCCG 1 cut(s) 9
Eam1104I CTCTTC 3 cut(s) 460, 528, 556
EarI CTCTTC 3 cut(s) 460, 528, 556
EclXI CGGCCG 1 cut(s) 9
Eco130I CCWWGG 3 cut(s) 250, 287, 383
Eco52I CGGCCG 1 cut(s) 9
EcoNI CCTNNNNNAGG 1 cut(s) 123
EcoRII CCWGG 1 cut(s) 637
EcoT14I CCWWGG 3 cut(s) 250, 287, 383
EcoT22I ATGCAT 2 cut(s) 622, 626
ErhI CCWWGG 3 cut(s) 250, 287, 383
FaeI CATG 4 cut(s) 115, 358, 476, 624
FaqI GGGAC 1 cut(s) 523
FatI CATG 4 cut(s) 111, 354, 472, 620
Fnu4HI GCNGC 4 cut(s) 123, 135, 138, 247
FokI GGATG 2 cut(s) 440, 572
Fsp4HI GCNGC 4 cut(s) 123, 135, 138, 247
GluI GCNGC 4 cut(s) 123, 135, 138, 247
HaeIII GGCC 3 cut(s) 11, 69, 236
HapII CCGG 1 cut(s) 201
Hin1II CATG 4 cut(s) 115, 358, 476, 624
HinfI GANTC 3 cut(s) 19, 80, 204
HpaII CCGG 1 cut(s) 201
HphI GGTGA 1 cut(s) 266
Hpy166II GTNNAC 2 cut(s) 344, 369
Hpy188I TCNGA 3 cut(s) 108, 298, 394
Hpy188III TCNNGA 4 cut(s) 201, 273, 497, 734
Hpy8I GTNNAC 2 cut(s) 344, 369
HpyAV CCTTC 3 cut(s) 36, 659, 715
HpyCH4IV ACGT 1 cut(s) 679
HpyCH4V TGCA 7 cut(s) 125, 162, 620, 624, 656, 662, 686
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 143
HpyF3I CTNAG 1 cut(s) 609
HpySE526I ACGT 1 cut(s) 679
Hsp92II CATG 4 cut(s) 115, 358, 476, 624
Kpn2I TCCGGA 1 cut(s) 200
KpnI GGTACC 1 cut(s) 762
Kzo9I GATC 3 cut(s) 114, 329, 547
LmnI GCTCC 1 cut(s) 131
Lsp1109I GCAGC 4 cut(s) 109, 146, 149, 258
MabI ACCWGGT 1 cut(s) 637
MaeII ACGT 1 cut(s) 679
MaeIII GTNAC 1 cut(s) 254
MalI GATC 3 cut(s) 116, 331, 549
MbiI CCGCTC 1 cut(s) 364
MboI GATC 3 cut(s) 114, 329, 547
MboII GAAGA 5 cut(s) 339, 477, 545, 573, 661
MfeI CAATTG 1 cut(s) 657
MflI RGATCY 2 cut(s) 329, 547
MlsI TGGCCA 2 cut(s) 69, 236
MluCI AATT 4 cut(s) 441, 514, 591, 657
MluNI TGGCCA 2 cut(s) 69, 236
MlyI GAGTC 2 cut(s) 13, 198
MmeI TCCRAC 1 cut(s) 538
MnlI CCTC 7 cut(s) 150, 253, 418, 461, 493, 529, 597
Mox20I TGGCCA 2 cut(s) 69, 236
Mph1103I ATGCAT 2 cut(s) 622, 626
MroI TCCGGA 1 cut(s) 200
MscI TGGCCA 2 cut(s) 69, 236
MseI TTAA 2 cut(s) 216, 518
MslI CAYNNNNRTG 1 cut(s) 41
Msp20I TGGCCA 2 cut(s) 69, 236
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 639
MunI CAATTG 1 cut(s) 657
MvaI CCWGG 1 cut(s) 639
MvnI CGCG 1 cut(s) 580
MwoI GCNNNNNNNGC 2 cut(s) 131, 143
NdeII GATC 3 cut(s) 114, 329, 547
NheI GCTAGC 1 cut(s) 526
NlaIII CATG 4 cut(s) 115, 358, 476, 624
NlaIV GGNNCC 4 cut(s) 295, 391, 549, 760
NmuCI GTSAC 1 cut(s) 254
NsiI ATGCAT 2 cut(s) 622, 626
NspI RCATGY 1 cut(s) 624
PaeI GCATGC 1 cut(s) 624
PfeI GAWTC 1 cut(s) 80
PkrI GCNGC 4 cut(s) 124, 136, 139, 248
PleI GAGTC 2 cut(s) 13, 198
PpsI GAGTC 2 cut(s) 13, 198
PsiI TTATAA 1 cut(s) 728
Psp6I CCWGG 1 cut(s) 637
PspGI CCWGG 1 cut(s) 637
PspN4I GGNNCC 4 cut(s) 295, 391, 549, 760
PstI CTGCAG 1 cut(s) 127
PsuI RGATCY 2 cut(s) 329, 547
RsaI GTAC 5 cut(s) 88, 169, 345, 760, 767
RsaNI GTAC 5 cut(s) 87, 168, 344, 759, 766
RseI CAYNNNNRTG 1 cut(s) 41
SaqAI TTAA 2 cut(s) 216, 518
SatI GCNGC 4 cut(s) 123, 135, 138, 247
Sau3AI GATC 3 cut(s) 114, 329, 547
ScaI AGTACT 1 cut(s) 169
SchI GAGTC 2 cut(s) 13, 198
ScrFI CCNGG 1 cut(s) 639
SexAI ACCWGGT 1 cut(s) 637
SfcI CTRYAG 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 41
SpeI ACTAGT 2 cut(s) 170, 179
SphI GCATGC 1 cut(s) 624
Sse9I AATT 4 cut(s) 441, 514, 591, 657
SsiI CCGC 1 cut(s) 364
StyD4I CCNGG 1 cut(s) 637
StyI CCWWGG 3 cut(s) 250, 287, 383
TaiI ACGT 1 cut(s) 682
TasI AATT 4 cut(s) 441, 514, 591, 657
TatI WGTACW 1 cut(s) 167
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 2 cut(s) 216, 518
Tru9I TTAA 2 cut(s) 216, 518
TscAI CASTG 1 cut(s) 43
TseFI GTSAC 1 cut(s) 254
TseI GCWGC 4 cut(s) 122, 134, 137, 246
Tsp45I GTSAC 1 cut(s) 254
TspDTI ATGAA 1 cut(s) 447
TspRI CASTG 1 cut(s) 43
XagI CCTNNNNNAGG 1 cut(s) 123
XapI RAATTY 2 cut(s) 441, 591
XbaI TCTAGA 1 cut(s) 496
XceI RCATGY 1 cut(s) 624
XmaJI CCTAGG 1 cut(s) 250
ZrmI AGTACT 1 cut(s) 169
Zsp2I ATGCAT 2 cut(s) 622, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.