FvH4_5g32140

Proteinase inhibitor type-2 CEVI57-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006762
Physical Location & Seq
Reverse (-)
23227668 .. 23228581
914 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32140.t1

Sequence Viewer

Length: 243 bp
ATGGCTAGTGACAAGAGTATCACTGCAATACTTCTTCTCCTTGTTTGTGGTTCAGTCATACTGGGAGTTGTTCCAACTGAAGCCAAAGTCTGCCCTTTTATTTGCTACGATGCAGCTGCTTATATGACCTGCCCGTCTTCAGGAAACGAGCAACTTTCACCTCCCTGCAACTGTTGCTTAGCGCCGGCACCAGGCTGCGCCCTTTACCGAGCTGATGGAACTCGCCTCTGTACTACTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prot_inhib_II

Protein Analysis

81

Amino Acids

8.25

Weight (kDa)

4.86

Isoelectric Point (pI)

56.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_inhib_II PF02428 29 - 78 4.5e-15 Potato type II proteinase inhibitor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 133
Acc36I ACCTGC 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 187
AcuI CTGAAG 2 cut(s) 99, 123
AfaI GTAC 1 cut(s) 232
AfiI CCNNNNNNNGG 2 cut(s) 140, 191
AjnI CCWGG 1 cut(s) 190
AluBI AGCT 2 cut(s) 116, 212
AluI AGCT 2 cut(s) 116, 212
ApeKI GCWGC 3 cut(s) 113, 116, 195
AspLEI GCGC 2 cut(s) 184, 200
AsuHPI GGTGA 1 cut(s) 150
BaeI ACNNNNGTAYC 1 cut(s) 34
BanI GGYRCC 1 cut(s) 187
BbsI GAAGAC 1 cut(s) 129
BbvI GCAGC 3 cut(s) 103, 125, 182
BccI CCATC 1 cut(s) 209
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BciT130I CCWGG 1 cut(s) 192
BfaI CTAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 185
BfuAI ACCTGC 1 cut(s) 137
BisI GCNGC 3 cut(s) 114, 117, 196
BlpI GCTNAGC 1 cut(s) 178
BlsI GCNGC 3 cut(s) 115, 118, 197
Bme1390I CCNGG 1 cut(s) 192
BmiI GGNNCC 1 cut(s) 189
BmrFI CCNGG 1 cut(s) 192
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 1 cut(s) 100
BmuI ACTGGG 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 129
Bpu1102I GCTNAGC 1 cut(s) 178
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 191
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 1 cut(s) 66
BseBI CCWGG 1 cut(s) 192
BseLI CCNNNNNNNGG 2 cut(s) 140, 191
BseNI ACTGG 1 cut(s) 66
BseXI GCAGC 3 cut(s) 103, 125, 182
BshNI GGYRCC 1 cut(s) 187
BsiSI CCGG 1 cut(s) 185
BslI CCNNNNNNNGG 2 cut(s) 140, 191
Bsp1720I GCTNAGC 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 189
BspMI ACCTGC 1 cut(s) 137
BspT107I GGYRCC 1 cut(s) 187
BsrFI RCCGGY 1 cut(s) 184
BsrI ACTGG 1 cut(s) 66
BssAI RCCGGY 1 cut(s) 184
Bst2UI CCWGG 1 cut(s) 192
Bst4CI ACNGT 1 cut(s) 173
BstAPI GCANNNNNTGC 1 cut(s) 174
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 1 cut(s) 178
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 2 cut(s) 184, 200
BstMWI GCNNNNNNNGC 1 cut(s) 174
BstNI CCWGG 1 cut(s) 192
BstSCI CCNGG 1 cut(s) 190
BstV1I GCAGC 3 cut(s) 103, 125, 182
BstV2I GAAGAC 1 cut(s) 129
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 1 cut(s) 21
BveI ACCTGC 1 cut(s) 137
Cac8I GCNNGC 1 cut(s) 186
CfoI GCGC 2 cut(s) 184, 200
Cfr10I RCCGGY 1 cut(s) 184
Csp6I GTAC 1 cut(s) 231
CviJI RGCY 5 cut(s) 5, 83, 116, 195, 212
CviKI_1 RGCY 5 cut(s) 5, 83, 116, 195, 212
CviQI GTAC 1 cut(s) 231
DdeI CTNAG 1 cut(s) 178
DrdI GACNNNNNNGTC 1 cut(s) 133
DseDI GACNNNNNNGTC 1 cut(s) 133
Eco57I CTGAAG 2 cut(s) 99, 123
EcoRII CCWGG 1 cut(s) 190
FaiI YATR 3 cut(s) 59, 123, 125
Fnu4HI GCNGC 3 cut(s) 114, 117, 196
Fsp4HI GCNGC 3 cut(s) 114, 117, 196
FspBI CTAG 1 cut(s) 6
GlaI GCGC 2 cut(s) 183, 199
GluI GCNGC 3 cut(s) 114, 117, 196
HaeII RGCGCY 1 cut(s) 185
HapII CCGG 1 cut(s) 185
HhaI GCGC 2 cut(s) 184, 200
Hin6I GCGC 2 cut(s) 182, 198
HinP1I GCGC 2 cut(s) 182, 198
HpaII CCGG 1 cut(s) 185
HphI GGTGA 1 cut(s) 150
Hpy188III TCNNGA 1 cut(s) 141
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4V TGCA 3 cut(s) 26, 113, 168
HpyF10VI GCNNNNNNNGC 1 cut(s) 174
HpyF3I CTNAG 1 cut(s) 178
HspAI GCGC 2 cut(s) 182, 198
KroI GCCGGC 1 cut(s) 184
KroNI GCCGGC 1 cut(s) 186
LpnPI CCDG 7 cut(s) 47, 126, 142, 177, 178, 198, 204
Lsp1109I GCAGC 3 cut(s) 103, 125, 182
LweI GCATC 1 cut(s) 100
MaeI CTAG 1 cut(s) 6
MaeIII GTNAC 1 cut(s) 8
MboII GAAGA 2 cut(s) 26, 129
MmeI TCCRAC 1 cut(s) 98
MnlI CCTC 2 cut(s) 171, 236
MroNI GCCGGC 1 cut(s) 184
MspA1I CMGCKG 1 cut(s) 116
MspI CCGG 1 cut(s) 185
MspR9I CCNGG 1 cut(s) 192
MvaI CCWGG 1 cut(s) 192
MwoI GCNNNNNNNGC 1 cut(s) 174
NaeI GCCGGC 1 cut(s) 186
NgoMIV GCCGGC 1 cut(s) 184
NlaIV GGNNCC 1 cut(s) 189
NmuCI GTSAC 1 cut(s) 8
PdiI GCCGGC 1 cut(s) 186
PkrI GCNGC 3 cut(s) 115, 118, 197
Psp6I CCWGG 1 cut(s) 190
PspGI CCWGG 1 cut(s) 190
PspN4I GGNNCC 1 cut(s) 189
PvuII CAGCTG 1 cut(s) 116
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
SatI GCNGC 3 cut(s) 114, 117, 196
ScrFI CCNGG 1 cut(s) 192
SetI ASST 5 cut(s) 118, 131, 163, 214, 242
SfaNI GCATC 1 cut(s) 100
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 1 cut(s) 190
TaaI ACNGT 1 cut(s) 173
TatI WGTACW 1 cut(s) 230
TscAI CASTG 1 cut(s) 28
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 3 cut(s) 113, 116, 195
Tsp45I GTSAC 1 cut(s) 8
TspRI CASTG 1 cut(s) 28
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.