RLG00000001573

Proteinase inhibitor type-2 CEVI57-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006762
Physical Location & Seq
Forward (+)
17709746 .. 17711759
2014 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001573

Sequence Viewer

Length: 375 bp
ATGGCTAGAGACAAAAGTATGGGTGCAATAGTTCTTCTTCTTGTTTGTGGTTCAATCCTACTGGGTATGAGTCCAACTGAGGCCTGCCCTCAATTTTGCTACGAGGCAGCTTATATGACTTGCCCGTCTTCAGGATCGACGCATATTTCTCCATCTTGCAACTGTTGCTTAGCTCCGGAGATTGCTGCAAAAAAGGCTCCAAACTTCCGATCAAATTTCAATGTCCCGCCAAGAGTTTTGCCTAAATTAGAATTCCTCGACTATATCGAATCTGGGAACAACAAACGAGGCCTCTGTTCTCTCACCCTCACCCAATTCATCATCCTTGCCATTCCATTCCAGATTCACCAAAACAAACACAGCTCGACCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prot_inhib_II

Protein Analysis

125

Amino Acids

13.48

Weight (kDa)

8.41

Isoelectric Point (pI)

69.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_inhib_II PF02428 28 - 59 3.5e-08 Potato type II proteinase inhibitor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 124
AccIII TCCGGA 1 cut(s) 175
AciI CCGC 1 cut(s) 227
AclWI GGATC 1 cut(s) 142
AcsI RAATTY 2 cut(s) 214, 251
AcuI CTGAAG 1 cut(s) 114
AfiI CCNNNNNNNGG 1 cut(s) 131
AgsI TTSAA 2 cut(s) 54, 220
AluBI AGCT 3 cut(s) 110, 173, 363
AluI AGCT 3 cut(s) 110, 173, 363
Alw26I GTCTC 1 cut(s) 3
AlwI GGATC 1 cut(s) 142
Aor13HI TCCGGA 1 cut(s) 175
AoxI GGCC 2 cut(s) 81, 289
ApeKI GCWGC 2 cut(s) 107, 185
ApoI RAATTY 2 cut(s) 214, 251
AsuHPI GGTGA 3 cut(s) 295, 301, 338
BbsI GAAGAC 1 cut(s) 120
BbvI GCAGC 2 cut(s) 119, 172
BccI CCATC 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 3
BfaI CTAG 1 cut(s) 6
BisI GCNGC 2 cut(s) 108, 186
BlpI GCTNAGC 1 cut(s) 169
BlsI GCNGC 2 cut(s) 109, 187
BmiI GGNNCC 1 cut(s) 198
BmrI ACTGGG 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 120
Bpu1102I GCTNAGC 1 cut(s) 169
BsaWI WCCGGW 1 cut(s) 175
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse1I ACTGG 1 cut(s) 66
BseAI TCCGGA 1 cut(s) 175
BseGI GGATG 1 cut(s) 321
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 1 cut(s) 66
BseXI GCAGC 2 cut(s) 119, 172
BshFI GGCC 2 cut(s) 83, 291
BsiSI CCGG 1 cut(s) 176
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 1 cut(s) 131
BsmAI GTCTC 1 cut(s) 3
BsmFI GGGAC 1 cut(s) 209
BsnI GGCC 2 cut(s) 83, 291
Bsp13I TCCGGA 1 cut(s) 175
Bsp143I GATC 2 cut(s) 134, 209
Bsp1720I GCTNAGC 1 cut(s) 169
BspACI CCGC 1 cut(s) 227
BspANI GGCC 2 cut(s) 83, 291
BspCNI CTCAG 1 cut(s) 70
BspEI TCCGGA 1 cut(s) 175
BspLI GGNNCC 1 cut(s) 198
BspPI GGATC 1 cut(s) 142
BsrI ACTGG 1 cut(s) 66
BssMI GATC 2 cut(s) 134, 209
Bst4CI ACNGT 1 cut(s) 164
BstAPI GCANNNNNTGC 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 2 cut(s) 78, 169
BstF5I GGATG 1 cut(s) 321
BstKTI GATC 2 cut(s) 137, 212
BstMAI GTCTC 1 cut(s) 3
BstMBI GATC 2 cut(s) 134, 209
BstMWI GCNNNNNNNGC 2 cut(s) 165, 194
BstV1I GCAGC 2 cut(s) 119, 172
BstV2I GAAGAC 1 cut(s) 120
BsuRI GGCC 2 cut(s) 83, 291
BtsCI GGATG 1 cut(s) 321
Cac8I GCNNGC 1 cut(s) 85
CseI GACGC 1 cut(s) 148
CviJI RGCY 7 cut(s) 5, 83, 110, 173, 197, 291, 363
CviKI_1 RGCY 7 cut(s) 5, 83, 110, 173, 197, 291, 363
DdeI CTNAG 2 cut(s) 78, 169
DpnI GATC 2 cut(s) 136, 211
DpnII GATC 2 cut(s) 134, 209
DrdI GACNNNNNNGTC 1 cut(s) 124
DseDI GACNNNNNNGTC 1 cut(s) 124
Eco147I AGGCCT 2 cut(s) 83, 291
Eco57I CTGAAG 1 cut(s) 114
EcoRI GAATTC 1 cut(s) 251
FaiI YATR 6 cut(s) 20, 68, 114, 116, 144, 264
FaqI GGGAC 1 cut(s) 209
FauI CCCGC 1 cut(s) 234
Fnu4HI GCNGC 2 cut(s) 108, 186
FokI GGATG 1 cut(s) 308
Fsp4HI GCNGC 2 cut(s) 108, 186
FspBI CTAG 1 cut(s) 6
GluI GCNGC 2 cut(s) 108, 186
HaeIII GGCC 2 cut(s) 83, 291
HapII CCGG 1 cut(s) 176
HgaI GACGC 1 cut(s) 148
HinfI GANTC 3 cut(s) 70, 269, 343
HpaII CCGG 1 cut(s) 176
HphI GGTGA 3 cut(s) 295, 301, 338
Hpy188I TCNGA 1 cut(s) 209
Hpy188III TCNNGA 3 cut(s) 132, 176, 340
Hpy99I CGWCG 1 cut(s) 142
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4V TGCA 3 cut(s) 26, 159, 188
HpyF10VI GCNNNNNNNGC 2 cut(s) 165, 194
HpyF3I CTNAG 2 cut(s) 78, 169
Kpn2I TCCGGA 1 cut(s) 175
Kzo9I GATC 2 cut(s) 134, 209
LmnI GCTCC 2 cut(s) 178, 202
LpnPI CCDG 6 cut(s) 47, 97, 117, 189, 258, 353
Lsp1109I GCAGC 2 cut(s) 119, 172
MaeI CTAG 1 cut(s) 6
MalI GATC 2 cut(s) 136, 211
MboI GATC 2 cut(s) 134, 209
MboII GAAGA 3 cut(s) 26, 29, 120
MluCI AATT 5 cut(s) 92, 214, 245, 251, 314
MlyI GAGTC 1 cut(s) 79
MmeI TCCRAC 1 cut(s) 98
MnlI CCTC 7 cut(s) 73, 97, 99, 266, 281, 302, 317
MroI TCCGGA 1 cut(s) 175
MspI CCGG 1 cut(s) 176
MwoI GCNNNNNNNGC 2 cut(s) 165, 194
NdeII GATC 2 cut(s) 134, 209
NlaIV GGNNCC 1 cut(s) 198
PceI AGGCCT 2 cut(s) 83, 291
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PfeI GAWTC 2 cut(s) 269, 343
PkrI GCNGC 2 cut(s) 109, 187
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 198
SatI GCNGC 2 cut(s) 108, 186
Sau3AI GATC 2 cut(s) 134, 209
SchI GAGTC 1 cut(s) 79
SetI ASST 4 cut(s) 112, 175, 365, 371
Sse9I AATT 5 cut(s) 92, 214, 245, 251, 314
SseBI AGGCCT 2 cut(s) 83, 291
SsiI CCGC 1 cut(s) 227
SspMI CTAG 1 cut(s) 6
StuI AGGCCT 2 cut(s) 83, 291
TaaI ACNGT 1 cut(s) 164
TaqI TCGA 4 cut(s) 137, 258, 267, 365
TasI AATT 5 cut(s) 92, 214, 245, 251, 314
TfiI GAWTC 2 cut(s) 269, 343
TseI GCWGC 2 cut(s) 107, 185
TspDTI ATGAA 1 cut(s) 307
XapI RAATTY 2 cut(s) 214, 251
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.