FvH4_5g32180

Proteinase inhibitor type-2 CEVI57-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006762
Physical Location & Seq
Reverse (-)
23234588 .. 23235710
1123 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32180.t1

Sequence Viewer

Length: 243 bp
ATGGCTACAGGCAAGAGTATTGGTGCAATAGTTCTTCTCCTTGTTTGTGGTTCAATCCTACTGGGTATGAATCCAACTGAGGCCAAAGTTTGCATTCAAGTTTGCTACGAGGCAGCTTATATGACTTGCCCGTCCTCAGGCTCTACTCATCTCTCACCATCCTGCAACTGTTGCTTAGCTCCGGAGGTCGGCTGCTCCATTTACAACTCCGATGGAACTAAGATCTGTACTGGTACTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prot_inhib_II

Protein Analysis

81

Amino Acids

8.18

Weight (kDa)

5.1

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_inhib_II PF02428 29 - 78 8.5e-14 Potato type II proteinase inhibitor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 130
AccIII TCCGGA 1 cut(s) 181
AfaI GTAC 2 cut(s) 229, 235
AfiI CCNNNNNNNGG 2 cut(s) 137, 188
AgsI TTSAA 2 cut(s) 54, 98
AluBI AGCT 2 cut(s) 116, 179
AluI AGCT 2 cut(s) 116, 179
Aor13HI TCCGGA 1 cut(s) 181
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 2 cut(s) 113, 192
AsuHPI GGTGA 1 cut(s) 147
AxyI CCTNAGG 1 cut(s) 136
BbvI GCAGC 2 cut(s) 125, 179
BccI CCATC 2 cut(s) 166, 206
BfmI CTRYAG 1 cut(s) 6
BglII AGATCT 1 cut(s) 222
BisI GCNGC 2 cut(s) 114, 193
BlpI GCTNAGC 1 cut(s) 175
BlsI GCNGC 2 cut(s) 115, 194
BmrI ACTGGG 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 71
Bpu1102I GCTNAGC 1 cut(s) 175
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 188
Bse1I ACTGG 2 cut(s) 66, 235
Bse21I CCTNAGG 1 cut(s) 136
BseAI TCCGGA 1 cut(s) 181
BseGI GGATG 1 cut(s) 158
BseLI CCNNNNNNNGG 2 cut(s) 137, 188
BseMII CTCAG 2 cut(s) 69, 150
BseNI ACTGG 2 cut(s) 66, 235
BseXI GCAGC 2 cut(s) 125, 179
BshFI GGCC 1 cut(s) 83
BsiSI CCGG 1 cut(s) 182
BslI CCNNNNNNNGG 2 cut(s) 137, 188
BsmI GAATGC 1 cut(s) 93
BsnI GGCC 1 cut(s) 83
Bsp13I TCCGGA 1 cut(s) 181
Bsp143I GATC 1 cut(s) 222
Bsp1720I GCTNAGC 1 cut(s) 175
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 2 cut(s) 70, 149
BspEI TCCGGA 1 cut(s) 181
BsrI ACTGG 2 cut(s) 66, 235
BssMI GATC 1 cut(s) 222
Bst4CI ACNGT 1 cut(s) 170
BstAPI GCANNNNNTGC 1 cut(s) 171
BstDEI CTNAG 4 cut(s) 78, 136, 175, 219
BstF5I GGATG 1 cut(s) 158
BstKTI GATC 1 cut(s) 225
BstMBI GATC 1 cut(s) 222
BstMWI GCNNNNNNNGC 1 cut(s) 171
BstSFI CTRYAG 1 cut(s) 6
BstV1I GCAGC 2 cut(s) 125, 179
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
Bsu36I CCTNAGG 1 cut(s) 136
BsuRI GGCC 1 cut(s) 83
BtsCI GGATG 1 cut(s) 158
Csp6I GTAC 2 cut(s) 228, 234
CviJI RGCY 6 cut(s) 5, 83, 116, 141, 179, 192
CviKI_1 RGCY 6 cut(s) 5, 83, 116, 141, 179, 192
CviQI GTAC 2 cut(s) 228, 234
DdeI CTNAG 4 cut(s) 78, 136, 175, 219
DpnI GATC 1 cut(s) 224
DpnII GATC 1 cut(s) 222
DrdI GACNNNNNNGTC 1 cut(s) 130
DseDI GACNNNNNNGTC 1 cut(s) 130
Eco81I CCTNAGG 1 cut(s) 136
FaiI YATR 3 cut(s) 68, 120, 122
Fnu4HI GCNGC 2 cut(s) 114, 193
FokI GGATG 1 cut(s) 145
Fsp4HI GCNGC 2 cut(s) 114, 193
GluI GCNGC 2 cut(s) 114, 193
HaeIII GGCC 1 cut(s) 83
HapII CCGG 1 cut(s) 182
HinfI GANTC 1 cut(s) 70
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 147
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 1 cut(s) 182
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4V TGCA 3 cut(s) 26, 93, 165
HpyF10VI GCNNNNNNNGC 1 cut(s) 171
HpyF3I CTNAG 4 cut(s) 78, 136, 175, 219
Kpn2I TCCGGA 1 cut(s) 181
Kzo9I GATC 1 cut(s) 222
LmnI GCTCC 2 cut(s) 184, 200
LpnPI CCDG 5 cut(s) 47, 123, 175, 195, 216
Lsp1109I GCAGC 2 cut(s) 125, 179
MalI GATC 1 cut(s) 224
MboI GATC 1 cut(s) 222
MboII GAAGA 1 cut(s) 26
MflI RGATCY 1 cut(s) 222
MmeI TCCRAC 1 cut(s) 98
MnlI CCTC 4 cut(s) 73, 103, 145, 178
MroI TCCGGA 1 cut(s) 181
MspI CCGG 1 cut(s) 182
Mva1269I GAATGC 1 cut(s) 93
MwoI GCNNNNNNNGC 1 cut(s) 171
NdeII GATC 1 cut(s) 222
PctI GAATGC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 70
PkrI GCNGC 2 cut(s) 115, 194
PsuI RGATCY 1 cut(s) 222
RsaI GTAC 2 cut(s) 229, 235
RsaNI GTAC 2 cut(s) 228, 234
SatI GCNGC 2 cut(s) 114, 193
Sau3AI GATC 1 cut(s) 222
SetI ASST 3 cut(s) 118, 181, 189
SfcI CTRYAG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 170
TatI WGTACW 1 cut(s) 227
TfiI GAWTC 1 cut(s) 70
TseI GCWGC 2 cut(s) 113, 192
TspDTI ATGAA 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.