Rh7DG397000

Proteinase inhibitor type-2 CEVI57-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0006762
Physical Location & Seq
Reverse (-)
54419413 .. 54420485
1073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG397000.1

Sequence Viewer

Length: 240 bp
ATGGCTAGAGACAAGAGTATGGGTGCAATAGTTCTTCTCCTTGTTTGTGGTTCAATCCTACTGGGTATGAGTCCAACTGAGGCCAAAGTTTGCCCTCAAATTTGCTACGAGGCAGCTTATATGACTTGCCCGTCTTCAGGATCGACGCATCTTTCGCCATCTTGCAACTGTTGCTTAGCTCCGGAGGTTGGCTGCAAAATTTACAATTCTGATGGAACTCGAATCTGTACTAGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prot_inhib_II

Protein Analysis

79

Amino Acids

8.25

Weight (kDa)

6.5

Isoelectric Point (pI)

59.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_inhib_II PF02428 29 - 77 7.8e-15 Potato type II proteinase inhibitor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 130
AccIII TCCGGA 1 cut(s) 181
AclWI GGATC 1 cut(s) 148
AcsI RAATTY 2 cut(s) 99, 198
AcuI CTGAAG 1 cut(s) 120
AfaI GTAC 2 cut(s) 229, 235
AfiI CCNNNNNNNGG 2 cut(s) 137, 188
AgsI TTSAA 1 cut(s) 54
AhlI ACTAGT 1 cut(s) 230
AluBI AGCT 2 cut(s) 116, 179
AluI AGCT 2 cut(s) 116, 179
Alw26I GTCTC 1 cut(s) 3
AlwI GGATC 1 cut(s) 148
Aor13HI TCCGGA 1 cut(s) 181
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 2 cut(s) 113, 192
ApoI RAATTY 2 cut(s) 99, 198
ArsI GACNNNNNNTTYG 2 cut(s) 136, 168
BbsI GAAGAC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 125, 179
BccI CCATC 2 cut(s) 166, 206
BcoDI GTCTC 1 cut(s) 3
BcuI ACTAGT 1 cut(s) 230
BfaI CTAG 2 cut(s) 6, 231
BisI GCNGC 2 cut(s) 114, 193
BlpI GCTNAGC 1 cut(s) 175
BlsI GCNGC 2 cut(s) 115, 194
BmcAI AGTACT 1 cut(s) 235
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 1 cut(s) 157
BmuI ACTGGG 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 126
Bpu1102I GCTNAGC 1 cut(s) 175
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 188
Bse1I ACTGG 1 cut(s) 66
BseAI TCCGGA 1 cut(s) 181
BseLI CCNNNNNNNGG 2 cut(s) 137, 188
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 1 cut(s) 66
BseXI GCAGC 2 cut(s) 125, 179
BshFI GGCC 1 cut(s) 83
BsiSI CCGG 1 cut(s) 182
BslI CCNNNNNNNGG 2 cut(s) 137, 188
BsmAI GTCTC 1 cut(s) 3
BsnI GGCC 1 cut(s) 83
Bsp13I TCCGGA 1 cut(s) 181
Bsp143I GATC 1 cut(s) 140
Bsp1720I GCTNAGC 1 cut(s) 175
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 1 cut(s) 70
BspEI TCCGGA 1 cut(s) 181
BspPI GGATC 1 cut(s) 148
BsrI ACTGG 1 cut(s) 66
BssMI GATC 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 170
BstAPI GCANNNNNTGC 1 cut(s) 171
BstDEI CTNAG 2 cut(s) 78, 175
BstKTI GATC 1 cut(s) 143
BstMAI GTCTC 1 cut(s) 3
BstMBI GATC 1 cut(s) 140
BstMWI GCNNNNNNNGC 2 cut(s) 154, 171
BstV1I GCAGC 2 cut(s) 125, 179
BstV2I GAAGAC 1 cut(s) 126
BsuRI GGCC 1 cut(s) 83
CseI GACGC 1 cut(s) 154
Csp6I GTAC 2 cut(s) 228, 234
CviJI RGCY 5 cut(s) 5, 83, 116, 179, 192
CviKI_1 RGCY 5 cut(s) 5, 83, 116, 179, 192
CviQI GTAC 2 cut(s) 228, 234
DdeI CTNAG 2 cut(s) 78, 175
DpnI GATC 1 cut(s) 142
DpnII GATC 1 cut(s) 140
DrdI GACNNNNNNGTC 1 cut(s) 130
DseDI GACNNNNNNGTC 1 cut(s) 130
Eco57I CTGAAG 1 cut(s) 120
FaiI YATR 4 cut(s) 20, 68, 120, 122
Fnu4HI GCNGC 2 cut(s) 114, 193
Fsp4HI GCNGC 2 cut(s) 114, 193
FspBI CTAG 2 cut(s) 6, 231
GluI GCNGC 2 cut(s) 114, 193
HaeIII GGCC 1 cut(s) 83
HapII CCGG 1 cut(s) 182
HgaI GACGC 1 cut(s) 154
HinfI GANTC 2 cut(s) 70, 222
HpaII CCGG 1 cut(s) 182
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 2 cut(s) 138, 182
Hpy99I CGWCG 1 cut(s) 148
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4V TGCA 3 cut(s) 26, 165, 195
HpyF10VI GCNNNNNNNGC 2 cut(s) 154, 171
HpyF3I CTNAG 2 cut(s) 78, 175
Kpn2I TCCGGA 1 cut(s) 181
Kzo9I GATC 1 cut(s) 140
LmnI GCTCC 1 cut(s) 184
LpnPI CCDG 3 cut(s) 47, 123, 195
Lsp1109I GCAGC 2 cut(s) 125, 179
LweI GCATC 1 cut(s) 157
MaeI CTAG 2 cut(s) 6, 231
MalI GATC 1 cut(s) 142
MboI GATC 1 cut(s) 140
MboII GAAGA 2 cut(s) 26, 126
MluCI AATT 3 cut(s) 99, 198, 205
MlyI GAGTC 1 cut(s) 79
MmeI TCCRAC 1 cut(s) 98
MnlI CCTC 4 cut(s) 73, 103, 105, 178
MroI TCCGGA 1 cut(s) 181
MspI CCGG 1 cut(s) 182
MwoI GCNNNNNNNGC 2 cut(s) 154, 171
NdeII GATC 1 cut(s) 140
PfeI GAWTC 1 cut(s) 222
PkrI GCNGC 2 cut(s) 115, 194
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
RsaI GTAC 2 cut(s) 229, 235
RsaNI GTAC 2 cut(s) 228, 234
SatI GCNGC 2 cut(s) 114, 193
Sau3AI GATC 1 cut(s) 140
ScaI AGTACT 1 cut(s) 235
SchI GAGTC 1 cut(s) 79
SetI ASST 3 cut(s) 118, 181, 189
SfaNI GCATC 1 cut(s) 157
SpeI ACTAGT 1 cut(s) 230
Sse9I AATT 3 cut(s) 99, 198, 205
SspMI CTAG 2 cut(s) 6, 231
TaaI ACNGT 1 cut(s) 170
TaqI TCGA 2 cut(s) 143, 220
TasI AATT 3 cut(s) 99, 198, 205
TatI WGTACW 2 cut(s) 227, 233
TfiI GAWTC 1 cut(s) 222
TseI GCWGC 2 cut(s) 113, 192
XapI RAATTY 2 cut(s) 99, 198
XspI CTAG 2 cut(s) 6, 231
ZrmI AGTACT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.