RchiOBHm_Chr7g0228221

Proteinase inhibitor type-2 CEVI57-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0006762
Physical Location & Seq
Reverse (-)
51566364 .. 51566615
252 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20442

Sequence Viewer

Length: 252 bp
ATGTGCATGCATGTTCACAAAGGTTTTTGCTCGATTATGAGGGTTGATGTATATGCAGGTTCAATCATACTGGGAGGGATTCCAACTGAAGCCAAAGTTTGCCCTCTAATTTGCTATGATGTAGCTGCTTATATGACTTGCCCGTCTTCAGGAAACGAGCATCTTTCACCACTCTGCAACTGTTGCTTAGCATCGGAACCAGGCTGCGCCATTTACCTGTCCGATGGAACTCGCCTCTGTACTAGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prot_inhib_II

Protein Analysis

83

Amino Acids

8.8

Weight (kDa)

5.77

Isoelectric Point (pI)

55.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_inhib_II PF02428 32 - 81 7.2e-15 Potato type II proteinase inhibitor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 142
Acc36I ACCTGC 1 cut(s) 47
AcuI CTGAAG 2 cut(s) 108, 132
AfaI GTAC 2 cut(s) 241, 247
AfiI CCNNNNNNNGG 1 cut(s) 149
AgsI TTSAA 1 cut(s) 63
AhlI ACTAGT 1 cut(s) 242
AjnI CCWGG 1 cut(s) 199
AluBI AGCT 1 cut(s) 125
AluI AGCT 1 cut(s) 125
ApeKI GCWGC 2 cut(s) 125, 204
AspLEI GCGC 1 cut(s) 209
AsuHPI GGTGA 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 112, 191
BccI CCATC 1 cut(s) 218
BciT130I CCWGG 1 cut(s) 201
BcuI ACTAGT 1 cut(s) 242
BfaI CTAG 1 cut(s) 243
BfuAI ACCTGC 1 cut(s) 47
BisI GCNGC 2 cut(s) 126, 205
BlpI GCTNAGC 1 cut(s) 187
BlsI GCNGC 2 cut(s) 127, 206
BmcAI AGTACT 1 cut(s) 247
Bme1390I CCNGG 1 cut(s) 201
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 201
BmrI ACTGGG 1 cut(s) 80
BmsI GCATC 2 cut(s) 169, 200
BmuI ACTGGG 1 cut(s) 80
BpiI GAAGAC 1 cut(s) 138
Bpu1102I GCTNAGC 1 cut(s) 187
Bsc4I CCNNNNNNNGG 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 75
BseBI CCWGG 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 149
BseNI ACTGG 1 cut(s) 75
BseXI GCAGC 2 cut(s) 112, 191
BslI CCNNNNNNNGG 1 cut(s) 149
Bsp1720I GCTNAGC 1 cut(s) 187
BspLI GGNNCC 1 cut(s) 198
BspMI ACCTGC 1 cut(s) 47
BsrI ACTGG 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 201
Bst4CI ACNGT 1 cut(s) 182
BstAPI GCANNNNNTGC 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 8
BstDEI CTNAG 1 cut(s) 187
BstHHI GCGC 1 cut(s) 209
BstMWI GCNNNNNNNGC 1 cut(s) 183
BstNI CCWGG 1 cut(s) 201
BstNSI RCATGY 2 cut(s) 10, 14
BstSCI CCNGG 1 cut(s) 199
BstV1I GCAGC 2 cut(s) 112, 191
BstV2I GAAGAC 1 cut(s) 138
BveI ACCTGC 1 cut(s) 47
Cac8I GCNNGC 1 cut(s) 8
CfoI GCGC 1 cut(s) 209
Csp6I GTAC 2 cut(s) 240, 246
CviAII CATG 2 cut(s) 7, 11
CviJI RGCY 3 cut(s) 92, 125, 204
CviKI_1 RGCY 3 cut(s) 92, 125, 204
CviQI GTAC 2 cut(s) 240, 246
DdeI CTNAG 1 cut(s) 187
DrdI GACNNNNNNGTC 1 cut(s) 142
DseDI GACNNNNNNGTC 1 cut(s) 142
Eco57I CTGAAG 2 cut(s) 108, 132
EcoRII CCWGG 1 cut(s) 199
EcoT22I ATGCAT 1 cut(s) 12
FaeI CATG 2 cut(s) 10, 14
FaiI YATR 9 cut(s) 8, 12, 38, 52, 54, 68, 117, 132, 134
FatI CATG 2 cut(s) 6, 10
Fnu4HI GCNGC 2 cut(s) 126, 205
Fsp4HI GCNGC 2 cut(s) 126, 205
FspBI CTAG 1 cut(s) 243
GlaI GCGC 1 cut(s) 208
GluI GCNGC 2 cut(s) 126, 205
HhaI GCGC 1 cut(s) 209
Hin1II CATG 2 cut(s) 10, 14
Hin6I GCGC 1 cut(s) 207
HinP1I GCGC 1 cut(s) 207
HinfI GANTC 1 cut(s) 79
HphI GGTGA 1 cut(s) 159
Hpy166II GTNNAC 1 cut(s) 16
Hpy188I TCNGA 2 cut(s) 196, 223
Hpy188III TCNNGA 1 cut(s) 150
Hpy8I GTNNAC 1 cut(s) 16
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4V TGCA 4 cut(s) 6, 10, 56, 177
HpyF10VI GCNNNNNNNGC 1 cut(s) 183
HpyF3I CTNAG 1 cut(s) 187
Hsp92II CATG 2 cut(s) 10, 14
HspAI GCGC 1 cut(s) 207
LpnPI CCDG 6 cut(s) 42, 56, 135, 186, 213, 230
Lsp1109I GCAGC 2 cut(s) 112, 191
LweI GCATC 2 cut(s) 169, 200
MaeI CTAG 1 cut(s) 243
MboII GAAGA 1 cut(s) 138
MluCI AATT 1 cut(s) 108
MmeI TCCRAC 1 cut(s) 107
MnlI CCTC 4 cut(s) 33, 68, 114, 245
Mph1103I ATGCAT 1 cut(s) 12
MspR9I CCNGG 1 cut(s) 201
MvaI CCWGG 1 cut(s) 201
MwoI GCNNNNNNNGC 1 cut(s) 183
NlaIII CATG 2 cut(s) 10, 14
NlaIV GGNNCC 1 cut(s) 198
NsiI ATGCAT 1 cut(s) 12
NspI RCATGY 2 cut(s) 10, 14
PaeI GCATGC 1 cut(s) 10
PfeI GAWTC 1 cut(s) 79
PkrI GCNGC 2 cut(s) 127, 206
Psp6I CCWGG 1 cut(s) 199
PspGI CCWGG 1 cut(s) 199
PspN4I GGNNCC 1 cut(s) 198
RsaI GTAC 2 cut(s) 241, 247
RsaNI GTAC 2 cut(s) 240, 246
SatI GCNGC 2 cut(s) 126, 205
ScaI AGTACT 1 cut(s) 247
ScrFI CCNGG 1 cut(s) 201
SetI ASST 4 cut(s) 25, 61, 127, 219
SfaNI GCATC 2 cut(s) 169, 200
SpeI ACTAGT 1 cut(s) 242
SphI GCATGC 1 cut(s) 10
Sse9I AATT 1 cut(s) 108
SspMI CTAG 1 cut(s) 243
StyD4I CCNGG 1 cut(s) 199
TaaI ACNGT 1 cut(s) 182
TaqI TCGA 1 cut(s) 32
TasI AATT 1 cut(s) 108
TatI WGTACW 2 cut(s) 239, 245
TfiI GAWTC 1 cut(s) 79
TseI GCWGC 2 cut(s) 125, 204
XceI RCATGY 2 cut(s) 10, 14
XspI CTAG 1 cut(s) 243
ZrmI AGTACT 1 cut(s) 247
Zsp2I ATGCAT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.