Rh7CG420300

Proteinase inhibitor type-2 CEVI57-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0006762
Physical Location & Seq
Reverse (-)
54187122 .. 54196928
9807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG420300.1

Sequence Viewer

Length: 477 bp
ATGGACTCGGGCGGGTCGAGGATGACGGTGCTGGGGATAGAGAGACGAGTATTCCTTCAATTGCTGATTCCAGTCGTCGTCGTAGAGCTTGTTGATGATGCAGTCGAAGGGGCCTTGCTGGGTCAAGGCCTTGGCTGCATCGACGGGGACCAGATCAATGTCGTGTTGTCCGGCGTGGGCGATCAGCGACGGTTGGATGAAGGCGGCCTCTCCTCACAGCCTCAAAACCCGAAGCAAATTAACATGGCTAGAGACAAAAGTATGGGTGCAATAGTTCTTCTTCTTGTTTGTGGTTCAATCCTACTGGGAATGAGTCCAACTGAGGCCTGCCCTCAAATTTGCTACGAGGCAGCTTATATGACTTGCCCGTCTTCAGGATCGACGCATATTTCTCCATCTTGCAACTGTTGCTTAGCTCCGGAGGTTGGCTGCAAAATTTACTACTCTGATGGAACTCTAATCTGTACTAAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prot_inhib_II

Protein Analysis

158

Amino Acids

16.61

Weight (kDa)

4.59

Isoelectric Point (pI)

62.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_inhib_II PF02428 109 - 156 1.1e-13 Potato type II proteinase inhibitor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 367
AccIII TCCGGA 1 cut(s) 418
AciI CCGC 2 cut(s) 12, 204
AclWI GGATC 1 cut(s) 385
AcsI RAATTY 2 cut(s) 336, 435
AcuI CTGAAG 1 cut(s) 357
AfaI GTAC 1 cut(s) 466
AfiI CCNNNNNNNGG 2 cut(s) 374, 425
AgsI TTSAA 2 cut(s) 59, 297
AluBI AGCT 3 cut(s) 88, 353, 416
AluI AGCT 3 cut(s) 88, 353, 416
Alw26I GTCTC 2 cut(s) 37, 246
AlwI GGATC 1 cut(s) 385
Ama87I CYCGRG 1 cut(s) 7
Aor13HI TCCGGA 1 cut(s) 418
AoxI GGCC 4 cut(s) 111, 127, 205, 324
ApeKI GCWGC 3 cut(s) 135, 350, 429
ApoI RAATTY 2 cut(s) 336, 435
AspS9I GGNCC 2 cut(s) 111, 148
AvaI CYCGRG 1 cut(s) 7
AvaII GGWCC 1 cut(s) 148
BbsI GAAGAC 1 cut(s) 363
BbvI GCAGC 3 cut(s) 122, 362, 416
BccI CCATC 2 cut(s) 403, 443
BcoDI GTCTC 2 cut(s) 37, 246
BfaI CTAG 1 cut(s) 249
BisI GCNGC 4 cut(s) 136, 205, 351, 430
BlpI GCTNAGC 1 cut(s) 412
BlsI GCNGC 4 cut(s) 137, 206, 352, 431
Bme18I GGWCC 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 111, 148
BmiI GGNNCC 2 cut(s) 112, 149
BmrI ACTGGG 1 cut(s) 314
BmsI GCATC 2 cut(s) 88, 147
BmuI ACTGGG 1 cut(s) 314
BpiI GAAGAC 1 cut(s) 363
Bpu1102I GCTNAGC 1 cut(s) 412
BsaJI CCNNGG 1 cut(s) 130
BsaWI WCCGGW 1 cut(s) 418
Bsc4I CCNNNNNNNGG 2 cut(s) 374, 425
Bse1I ACTGG 2 cut(s) 71, 309
BseAI TCCGGA 1 cut(s) 418
BseDI CCNNGG 1 cut(s) 130
BseGI GGATG 2 cut(s) 27, 202
BseLI CCNNNNNNNGG 2 cut(s) 374, 425
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 2 cut(s) 71, 309
BseRI GAGGAG 1 cut(s) 202
BseXI GCAGC 3 cut(s) 122, 362, 416
BseYI CCCAGC 2 cut(s) 31, 118
BshFI GGCC 4 cut(s) 113, 129, 207, 326
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 2 cut(s) 171, 419
BslFI GGGAC 1 cut(s) 161
BslI CCNNNNNNNGG 2 cut(s) 374, 425
BsmAI GTCTC 2 cut(s) 37, 246
BsmBI CGTCTC 1 cut(s) 37
BsmFI GGGAC 1 cut(s) 161
BsnI GGCC 4 cut(s) 113, 129, 207, 326
BsoBI CYCGRG 1 cut(s) 7
Bsp13I TCCGGA 1 cut(s) 418
Bsp143I GATC 3 cut(s) 153, 181, 377
Bsp1720I GCTNAGC 1 cut(s) 412
BspACI CCGC 2 cut(s) 12, 204
BspANI GGCC 4 cut(s) 113, 129, 207, 326
BspCNI CTCAG 1 cut(s) 313
BspEI TCCGGA 1 cut(s) 418
BspLI GGNNCC 2 cut(s) 112, 149
BspPI GGATC 1 cut(s) 385
BsrI ACTGG 2 cut(s) 71, 309
BssECI CCNNGG 1 cut(s) 130
BssMI GATC 3 cut(s) 153, 181, 377
BssT1I CCWWGG 1 cut(s) 130
Bst4CI ACNGT 3 cut(s) 28, 192, 407
BstAPI GCANNNNNTGC 1 cut(s) 408
BstC8I GCNNGC 1 cut(s) 328
BstDEI CTNAG 2 cut(s) 321, 412
BstF5I GGATG 2 cut(s) 27, 202
BstKTI GATC 3 cut(s) 156, 184, 380
BstMAI GTCTC 2 cut(s) 37, 246
BstMBI GATC 3 cut(s) 153, 181, 377
BstMWI GCNNNNNNNGC 2 cut(s) 135, 408
BstV1I GCAGC 3 cut(s) 122, 362, 416
BstV2I GAAGAC 1 cut(s) 363
BsuRI GGCC 4 cut(s) 113, 129, 207, 326
BtsCI GGATG 2 cut(s) 27, 202
Cac8I GCNNGC 1 cut(s) 328
Cfr13I GGNCC 2 cut(s) 111, 148
CseI GACGC 1 cut(s) 391
Csp6I GTAC 1 cut(s) 465
CviAII CATG 1 cut(s) 244
CviQI GTAC 1 cut(s) 465
DdeI CTNAG 2 cut(s) 321, 412
DpnI GATC 3 cut(s) 155, 183, 379
DpnII GATC 3 cut(s) 153, 181, 377
DrdI GACNNNNNNGTC 1 cut(s) 367
DseDI GACNNNNNNGTC 1 cut(s) 367
Eco130I CCWWGG 1 cut(s) 130
Eco147I AGGCCT 2 cut(s) 129, 326
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 357
Eco88I CYCGRG 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 130
Esp3I CGTCTC 1 cut(s) 37
FaeI CATG 1 cut(s) 247
FaiI YATR 5 cut(s) 245, 263, 357, 359, 387
FaqI GGGAC 1 cut(s) 161
FatI CATG 1 cut(s) 243
FauI CCCGC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 136, 205, 351, 430
FokI GGATG 2 cut(s) 34, 209
Fsp4HI GCNGC 4 cut(s) 136, 205, 351, 430
FspBI CTAG 1 cut(s) 249
GluI GCNGC 4 cut(s) 136, 205, 351, 430
GsaI CCCAGC 2 cut(s) 35, 122
HaeIII GGCC 4 cut(s) 113, 129, 207, 326
HapII CCGG 2 cut(s) 171, 419
HgaI GACGC 1 cut(s) 391
Hin1II CATG 1 cut(s) 247
HinfI GANTC 3 cut(s) 5, 67, 313
HpaII CCGG 2 cut(s) 171, 419
Hpy188I TCNGA 1 cut(s) 448
Hpy188III TCNNGA 2 cut(s) 375, 419
Hpy99I CGWCG 5 cut(s) 80, 83, 146, 192, 385
HpyAV CCTTC 3 cut(s) 65, 101, 194
HpyCH4III ACNGT 3 cut(s) 28, 192, 407
HpyCH4V TGCA 5 cut(s) 101, 138, 269, 402, 432
HpyF10VI GCNNNNNNNGC 2 cut(s) 135, 408
HpyF3I CTNAG 2 cut(s) 321, 412
Hsp92II CATG 1 cut(s) 247
Kpn2I TCCGGA 1 cut(s) 418
Kzo9I GATC 3 cut(s) 153, 181, 377
LmnI GCTCC 1 cut(s) 421
LpnPI CCDG 9 cut(s) 17, 84, 104, 164, 184, 290, 340, 360, 432
Lsp1109I GCAGC 3 cut(s) 122, 362, 416
LweI GCATC 2 cut(s) 88, 147
MaeI CTAG 1 cut(s) 249
MalI GATC 3 cut(s) 155, 183, 379
MboI GATC 3 cut(s) 153, 181, 377
MboII GAAGA 3 cut(s) 269, 272, 363
MfeI CAATTG 1 cut(s) 59
MluCI AATT 4 cut(s) 59, 237, 336, 435
MlyI GAGTC 1 cut(s) 322
MmeI TCCRAC 2 cut(s) 174, 341
MnlI CCTC 8 cut(s) 12, 218, 223, 231, 316, 340, 342, 415
MroI TCCGGA 1 cut(s) 418
MseI TTAA 1 cut(s) 240
MspI CCGG 2 cut(s) 171, 419
MunI CAATTG 1 cut(s) 59
MwoI GCNNNNNNNGC 2 cut(s) 135, 408
NdeII GATC 3 cut(s) 153, 181, 377
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 2 cut(s) 112, 149
PceI AGGCCT 2 cut(s) 129, 326
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PfeI GAWTC 1 cut(s) 67
PkrI GCNGC 4 cut(s) 137, 206, 352, 431
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
PspFI CCCAGC 2 cut(s) 31, 118
PspN4I GGNNCC 2 cut(s) 112, 149
PspPI GGNCC 2 cut(s) 111, 148
RsaI GTAC 1 cut(s) 466
RsaNI GTAC 1 cut(s) 465
SaqAI TTAA 1 cut(s) 240
SatI GCNGC 4 cut(s) 136, 205, 351, 430
Sau3AI GATC 3 cut(s) 153, 181, 377
Sau96I GGNCC 2 cut(s) 111, 148
SchI GAGTC 1 cut(s) 322
SetI ASST 4 cut(s) 90, 355, 418, 426
SfaNI GCATC 2 cut(s) 88, 147
SinI GGWCC 1 cut(s) 148
Sse9I AATT 4 cut(s) 59, 237, 336, 435
SseBI AGGCCT 2 cut(s) 129, 326
SsiI CCGC 2 cut(s) 12, 204
SspMI CTAG 1 cut(s) 249
StuI AGGCCT 2 cut(s) 129, 326
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 3 cut(s) 28, 192, 407
TaqI TCGA 4 cut(s) 17, 105, 141, 380
TasI AATT 4 cut(s) 59, 237, 336, 435
TatI WGTACW 1 cut(s) 464
TauI GCSGC 1 cut(s) 207
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TseI GCWGC 3 cut(s) 135, 350, 429
TspDTI ATGAA 1 cut(s) 213
VpaK11BI GGWCC 1 cut(s) 148
XapI RAATTY 2 cut(s) 336, 435
XspI CTAG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.