Rroxscaffold_3G00231260

Proteinase inhibitor type-2 CEVI57-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006762
Physical Location & Seq
Forward (+)
16346033 .. 16346569
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00231260.1

Sequence Viewer

Length: 234 bp
ATGGCTAGAGACAAAAGTATGGGTGCAATAGTTCTTCTTCTTGTTTGTGGTTCAATCCTACTGGGTATGAGTCCAACTGAGGCCTGCCCTCAAATTTGCTACGAGGCAGCTTATATGACTTGCCCGTCTTCAGGATCGACGCATATTTCTCCATCTTGCAACTGTTGCTTAGCTCCGGAGGTTGGCTGCAAAATTTACTACTCTGATGGAACTCTAATCTGTACTAAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prot_inhib_II

Protein Analysis

77

Amino Acids

8.07

Weight (kDa)

5.54

Isoelectric Point (pI)

74.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_inhib_II PF02428 28 - 75 2e-14 Potato type II proteinase inhibitor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 124
AccIII TCCGGA 1 cut(s) 175
AclWI GGATC 1 cut(s) 142
AcsI RAATTY 2 cut(s) 93, 192
AcuI CTGAAG 1 cut(s) 114
AfaI GTAC 1 cut(s) 223
AfiI CCNNNNNNNGG 2 cut(s) 131, 182
AgsI TTSAA 1 cut(s) 54
AluBI AGCT 2 cut(s) 110, 173
AluI AGCT 2 cut(s) 110, 173
Alw26I GTCTC 1 cut(s) 3
AlwI GGATC 1 cut(s) 142
Aor13HI TCCGGA 1 cut(s) 175
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 2 cut(s) 107, 186
ApoI RAATTY 2 cut(s) 93, 192
BbsI GAAGAC 1 cut(s) 120
BbvI GCAGC 2 cut(s) 119, 173
BccI CCATC 2 cut(s) 160, 200
BcoDI GTCTC 1 cut(s) 3
BfaI CTAG 1 cut(s) 6
BisI GCNGC 2 cut(s) 108, 187
BlpI GCTNAGC 1 cut(s) 169
BlsI GCNGC 2 cut(s) 109, 188
BmrI ACTGGG 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 120
Bpu1102I GCTNAGC 1 cut(s) 169
BsaWI WCCGGW 1 cut(s) 175
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 182
Bse1I ACTGG 1 cut(s) 66
BseAI TCCGGA 1 cut(s) 175
BseLI CCNNNNNNNGG 2 cut(s) 131, 182
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 1 cut(s) 66
BseXI GCAGC 2 cut(s) 119, 173
BshFI GGCC 1 cut(s) 83
BsiSI CCGG 1 cut(s) 176
BslI CCNNNNNNNGG 2 cut(s) 131, 182
BsmAI GTCTC 1 cut(s) 3
BsnI GGCC 1 cut(s) 83
Bsp13I TCCGGA 1 cut(s) 175
Bsp143I GATC 1 cut(s) 134
Bsp1720I GCTNAGC 1 cut(s) 169
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 1 cut(s) 70
BspEI TCCGGA 1 cut(s) 175
BspPI GGATC 1 cut(s) 142
BsrI ACTGG 1 cut(s) 66
BssMI GATC 1 cut(s) 134
Bst4CI ACNGT 1 cut(s) 164
BstAPI GCANNNNNTGC 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 2 cut(s) 78, 169
BstKTI GATC 1 cut(s) 137
BstMAI GTCTC 1 cut(s) 3
BstMBI GATC 1 cut(s) 134
BstMWI GCNNNNNNNGC 1 cut(s) 165
BstV1I GCAGC 2 cut(s) 119, 173
BstV2I GAAGAC 1 cut(s) 120
BsuRI GGCC 1 cut(s) 83
Cac8I GCNNGC 1 cut(s) 85
CseI GACGC 1 cut(s) 148
Csp6I GTAC 1 cut(s) 222
CviJI RGCY 5 cut(s) 5, 83, 110, 173, 186
CviKI_1 RGCY 5 cut(s) 5, 83, 110, 173, 186
CviQI GTAC 1 cut(s) 222
DdeI CTNAG 2 cut(s) 78, 169
DpnI GATC 1 cut(s) 136
DpnII GATC 1 cut(s) 134
DrdI GACNNNNNNGTC 1 cut(s) 124
DseDI GACNNNNNNGTC 1 cut(s) 124
Eco147I AGGCCT 1 cut(s) 83
Eco57I CTGAAG 1 cut(s) 114
FaiI YATR 5 cut(s) 20, 68, 114, 116, 144
Fnu4HI GCNGC 2 cut(s) 108, 187
Fsp4HI GCNGC 2 cut(s) 108, 187
FspBI CTAG 1 cut(s) 6
GluI GCNGC 2 cut(s) 108, 187
HaeIII GGCC 1 cut(s) 83
HapII CCGG 1 cut(s) 176
HgaI GACGC 1 cut(s) 148
HinfI GANTC 1 cut(s) 70
HpaII CCGG 1 cut(s) 176
Hpy188I TCNGA 1 cut(s) 205
Hpy188III TCNNGA 2 cut(s) 132, 176
Hpy99I CGWCG 1 cut(s) 142
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4V TGCA 3 cut(s) 26, 159, 189
HpyF10VI GCNNNNNNNGC 1 cut(s) 165
HpyF3I CTNAG 2 cut(s) 78, 169
Kpn2I TCCGGA 1 cut(s) 175
Kzo9I GATC 1 cut(s) 134
LmnI GCTCC 1 cut(s) 178
LpnPI CCDG 4 cut(s) 47, 97, 117, 189
Lsp1109I GCAGC 2 cut(s) 119, 173
MaeI CTAG 1 cut(s) 6
MalI GATC 1 cut(s) 136
MboI GATC 1 cut(s) 134
MboII GAAGA 3 cut(s) 26, 29, 120
MluCI AATT 2 cut(s) 93, 192
MlyI GAGTC 1 cut(s) 79
MmeI TCCRAC 1 cut(s) 98
MnlI CCTC 4 cut(s) 73, 97, 99, 172
MroI TCCGGA 1 cut(s) 175
MspI CCGG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 165
NdeII GATC 1 cut(s) 134
PceI AGGCCT 1 cut(s) 83
PkrI GCNGC 2 cut(s) 109, 188
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
RsaI GTAC 1 cut(s) 223
RsaNI GTAC 1 cut(s) 222
SatI GCNGC 2 cut(s) 108, 187
Sau3AI GATC 1 cut(s) 134
SchI GAGTC 1 cut(s) 79
SetI ASST 3 cut(s) 112, 175, 183
Sse9I AATT 2 cut(s) 93, 192
SseBI AGGCCT 1 cut(s) 83
SspMI CTAG 1 cut(s) 6
StuI AGGCCT 1 cut(s) 83
TaaI ACNGT 1 cut(s) 164
TaqI TCGA 1 cut(s) 137
TasI AATT 2 cut(s) 93, 192
TatI WGTACW 1 cut(s) 221
TseI GCWGC 2 cut(s) 107, 186
XapI RAATTY 2 cut(s) 93, 192
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.