Rh7AG401400

Proteinase inhibitor type-2 CEVI57-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0006762
Physical Location & Seq
Reverse (-)
52775032 .. 52785421
10390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG401400.1

Sequence Viewer

Length: 252 bp
ATGGCTAGAGACAAAAGTATGGGTGCAATAGTTCTTCTTCTTGTTTGTGGTTCAATCATACTGGGAGGGATTCCAACTGAAGCCAAAGTTTGCCCTCTAATTTGCTATGATGCAGCTGCTTATATGACTTGCCCGTCTTCAGGAAACGAGCATCTTTCACCACTCTGCAACTGTTGCTTAGCATCGGAACCAGGCTGCGCCCCCCGCGCCCTTTACCTGTCCGATGGAACTCGCCTCTGTACTAGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prot_inhib_II

Protein Analysis

83

Amino Acids

8.58

Weight (kDa)

5.56

Isoelectric Point (pI)

43.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_inhib_II PF02428 29 - 81 2.9e-13 Potato type II proteinase inhibitor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 133
AccII CGCG 1 cut(s) 207
AciI CCGC 1 cut(s) 205
AcuI CTGAAG 2 cut(s) 99, 123
AfaI GTAC 2 cut(s) 241, 247
AfiI CCNNNNNNNGG 1 cut(s) 140
AgsI TTSAA 1 cut(s) 54
AhlI ACTAGT 1 cut(s) 242
AjnI CCWGG 1 cut(s) 190
AluBI AGCT 1 cut(s) 116
AluI AGCT 1 cut(s) 116
Alw26I GTCTC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 113, 116, 195
AspLEI GCGC 2 cut(s) 200, 209
AsuHPI GGTGA 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 129
BbvI GCAGC 3 cut(s) 103, 125, 182
BccI CCATC 1 cut(s) 218
BciT130I CCWGG 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 3
BcuI ACTAGT 1 cut(s) 242
BfaI CTAG 2 cut(s) 6, 243
BisI GCNGC 3 cut(s) 114, 117, 196
BlpI GCTNAGC 1 cut(s) 178
BlsI GCNGC 3 cut(s) 115, 118, 197
BmcAI AGTACT 1 cut(s) 247
Bme1390I CCNGG 1 cut(s) 192
BmiI GGNNCC 1 cut(s) 189
BmrFI CCNGG 1 cut(s) 192
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 3 cut(s) 100, 160, 191
BmuI ACTGGG 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 129
Bpu1102I GCTNAGC 1 cut(s) 178
Bsc4I CCNNNNNNNGG 1 cut(s) 140
Bse1I ACTGG 1 cut(s) 66
BseBI CCWGG 1 cut(s) 192
BseLI CCNNNNNNNGG 1 cut(s) 140
BseNI ACTGG 1 cut(s) 66
BseXI GCAGC 3 cut(s) 103, 125, 182
Bsh1236I CGCG 1 cut(s) 207
BslI CCNNNNNNNGG 1 cut(s) 140
BsmAI GTCTC 1 cut(s) 3
Bsp1720I GCTNAGC 1 cut(s) 178
BspACI CCGC 1 cut(s) 205
BspFNI CGCG 1 cut(s) 207
BspLI GGNNCC 1 cut(s) 189
BsrI ACTGG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 192
Bst4CI ACNGT 1 cut(s) 173
BstAPI GCANNNNNTGC 1 cut(s) 174
BstDEI CTNAG 1 cut(s) 178
BstFNI CGCG 1 cut(s) 207
BstHHI GCGC 2 cut(s) 200, 209
BstMAI GTCTC 1 cut(s) 3
BstMWI GCNNNNNNNGC 3 cut(s) 174, 204, 206
BstNI CCWGG 1 cut(s) 192
BstSCI CCNGG 1 cut(s) 190
BstUI CGCG 1 cut(s) 207
BstV1I GCAGC 3 cut(s) 103, 125, 182
BstV2I GAAGAC 1 cut(s) 129
CfoI GCGC 2 cut(s) 200, 209
Csp6I GTAC 2 cut(s) 240, 246
CviJI RGCY 4 cut(s) 5, 83, 116, 195
CviKI_1 RGCY 4 cut(s) 5, 83, 116, 195
CviQI GTAC 2 cut(s) 240, 246
DdeI CTNAG 1 cut(s) 178
DrdI GACNNNNNNGTC 1 cut(s) 133
DseDI GACNNNNNNGTC 1 cut(s) 133
Eco57I CTGAAG 2 cut(s) 99, 123
EcoRII CCWGG 1 cut(s) 190
FaiI YATR 5 cut(s) 20, 59, 108, 123, 125
FauI CCCGC 1 cut(s) 212
Fnu4HI GCNGC 3 cut(s) 114, 117, 196
Fsp4HI GCNGC 3 cut(s) 114, 117, 196
FspBI CTAG 2 cut(s) 6, 243
GlaI GCGC 2 cut(s) 199, 208
GluI GCNGC 3 cut(s) 114, 117, 196
HhaI GCGC 2 cut(s) 200, 209
Hin6I GCGC 2 cut(s) 198, 207
HinP1I GCGC 2 cut(s) 198, 207
HinfI GANTC 1 cut(s) 70
HphI GGTGA 1 cut(s) 150
Hpy188I TCNGA 2 cut(s) 187, 223
Hpy188III TCNNGA 1 cut(s) 141
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4V TGCA 3 cut(s) 26, 113, 168
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 204, 206
HpyF3I CTNAG 1 cut(s) 178
HspAI GCGC 2 cut(s) 198, 207
LpnPI CCDG 5 cut(s) 47, 126, 177, 204, 230
Lsp1109I GCAGC 3 cut(s) 103, 125, 182
LweI GCATC 3 cut(s) 100, 160, 191
MaeI CTAG 2 cut(s) 6, 243
MboII GAAGA 3 cut(s) 26, 29, 129
MluCI AATT 1 cut(s) 99
MmeI TCCRAC 1 cut(s) 98
MnlI CCTC 3 cut(s) 59, 105, 245
MspA1I CMGCKG 1 cut(s) 116
MspR9I CCNGG 1 cut(s) 192
MvaI CCWGG 1 cut(s) 192
MvnI CGCG 1 cut(s) 207
MwoI GCNNNNNNNGC 3 cut(s) 174, 204, 206
NlaIV GGNNCC 1 cut(s) 189
PfeI GAWTC 1 cut(s) 70
PkrI GCNGC 3 cut(s) 115, 118, 197
Psp6I CCWGG 1 cut(s) 190
PspGI CCWGG 1 cut(s) 190
PspN4I GGNNCC 1 cut(s) 189
PvuII CAGCTG 1 cut(s) 116
RsaI GTAC 2 cut(s) 241, 247
RsaNI GTAC 2 cut(s) 240, 246
SatI GCNGC 3 cut(s) 114, 117, 196
ScaI AGTACT 1 cut(s) 247
ScrFI CCNGG 1 cut(s) 192
SetI ASST 2 cut(s) 118, 219
SfaNI GCATC 3 cut(s) 100, 160, 191
SpeI ACTAGT 1 cut(s) 242
Sse9I AATT 1 cut(s) 99
SsiI CCGC 1 cut(s) 205
SspMI CTAG 2 cut(s) 6, 243
StyD4I CCNGG 1 cut(s) 190
TaaI ACNGT 1 cut(s) 173
TasI AATT 1 cut(s) 99
TatI WGTACW 2 cut(s) 239, 245
TfiI GAWTC 1 cut(s) 70
TseI GCWGC 3 cut(s) 113, 116, 195
XspI CTAG 2 cut(s) 6, 243
ZrmI AGTACT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.