FvH4_5g35730

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002831
Physical Location & Seq
Reverse (-)
26172099 .. 26174681
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35730.t4

Sequence Viewer

Length: 1683 bp
ATGGCGAGAAGACTCCTCCTCAAAACACTTGCTTCTCGAGCTCTCTTGCCAATCCCAGACCGCTCCGACCGTCGGCGACCCACTTCAGCCGCTGAGCTCTTTCTGGGGCCGGGCCCTAGTAAAACAGTTGTAGTTTTCATGAATCTAAGGAGAGTTCATTTTAGCTGGCGTCGTTGGTTTGCTTCCTCTCCTTCGACAATCTCATCATCAGTTTCATGGATCTCTCCTCTTAAGTTATCAAAACTCAATGCTCACCAACCAGATCCCCCTCCTGATACACGGACAGAAGCTAGGAGGAAATCGAAATATATTTCTCACAATGCTGCCATCAACTTAATCAAACGTGAGAGAGATCCACAACATGCGTTGGAAATTTTTAACATGGTCTCGGAGCAAAAGGGTTTCAACCACAACAATGCCACCTATGCAACTATCCTCAATAAGCTTTCTCAGTCCAAGAAGTTCAAGGCTGTTGATGCAGTTCTCTATCAAATGAAGTATGATACCTGCAAATTTCATGAGGGTATATTCCTTAATCTCATGAAGCATTTTTCAAAATTTTCTATGCATGAGAGAGTGCTTGAAATGTTCCATGCTATCCAGCCAATAGTTCGGGAAAAGCCTTCTCTCAAATGCATTAGTACTTGTCTAAATCTGCTCATTGAAGCGAATCAGGTTGATATGGCCCAACAGTTTCTCATGCATTTGAAGAAGAGTCTTAACTTGAAGCTGAATACATGCATTGCCAACATCTTGGTTAAGCACTATTGCAAAAATGGGGATCTTGAATCTGCCTTTGAAGTTGTGAAGAAAATGAAAAAGTCTAAGCTCTCCTATCCTAATTTGATTACTTATTCAACACTAATTGATGGTCTATGTCAAAGTGGAAAACTGACAGAAGCAATGGACATGTTTGATGAAATGATCTCGAAGGAGCAAATATTGCCTGATGTCCTAACGTACAACATTTTGATGAAGGGGTTTTGCCGTGCTGGGAAAGTTGATAGGGCGAGGAAGATATTGGATTTTATGAAGAGCAAAGGATGCAACCCAAATATATACAATTACTCAACTCTGATGAATGGATTCTGTAAGGAGGTAAGGTTGAAAGAGGCCCAGGAGCTTTTGGATGAAATGAAGAGCTTTGGTATAAAACCAGATACAGTTGTCTACACTACTTTAATTGATTGCCACTGTAGGACTGGTAGAGTTGATGAAGCAATAGAGTTGCTCAAAGAGATGAAAGAAAGAAGGTGCAAAGCTGACACTGTGACTTTCAATGTCATACTTGGAGGATTATGTAGAGAATGTAGAATTGAGGATGCTCTTAAGATGCTGGACGAACTTCCTTACGAGGGTATTTATCTGAATAAGGGAAGCTACAGAATTGTGTTGAATTCCCTGTATCAGAAAGGTGACTTGAACAAAGCTAAGGAGTTGTTGAGATTGATGATGGGCAGGGGTTTTGTACCACATTATGCAACTTCAAACGGGTTGCTAGTTAGCCTTTGTGAGGCTGGAATGATAGATGATGCAACTACGGCTTTGTTTGGATTGGTGGAGATGGGATTCAAACCACTGCTTGATTCCTGGGCTTTTTTTGTTGAATCAATTTGCAGAGAAAGAAAGCTACTGCCTGCGTTTGAACTGCTCGATGAATTGGTTAATGAAGAGTTTGGTTAA

Protein Analysis

561

Amino Acids

64.07

Weight (kDa)

9.17

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 61 - 258 2e-12 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 127 - 159 9.7e-08 PPR repeat family
PPR_3 PF13812 139 - 192 3.9e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 143 - 174 1.6e-12 PPR repeat
PPR_2 PF13041 145 - 194 2.8e-18 PPR repeat family
PPR PF01535 149 - 178 1.6e-08 PPR repeat
PPR_1 PF12854 176 - 209 2.4e-12 PPR repeat
PPR_2 PF13041 181 - 225 2.9e-12 PPR repeat family
PPR PF01535 185 - 213 7.2e-07 PPR repeat
TPR_24 PF23276 201 - 281 1.7e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 204 - 260 4e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 212 - 244 1.3e-14 PPR repeat
PPR_2 PF13041 215 - 264 8.3e-19 PPR repeat family
PPR PF01535 218 - 248 1.9e-10 PPR repeat
PPR_1 PF12854 247 - 277 1.3e-07 PPR repeat
PPR_2 PF13041 251 - 297 1.1e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 515
AccBSI CCGCTC 1 cut(s) 63
AccI GTMKAC 1 cut(s) 1170
AciI CCGC 2 cut(s) 61, 90
AclWI GGATC 4 cut(s) 227, 257, 347, 789
AcsI RAATTY 4 cut(s) 372, 512, 557, 1396
AcuI CTGAAG 1 cut(s) 69
AcyI GRCGYC 1 cut(s) 169
AfaI GTAC 3 cut(s) 643, 962, 1470
AfiI CCNNNNNNNGG 3 cut(s) 72, 1355, 1513
AflII CTTAAG 2 cut(s) 230, 1328
AflIII ACRYGT 1 cut(s) 909
AjnI CCWGG 2 cut(s) 1116, 1589
Alw21I GWGCWC 2 cut(s) 43, 99
Alw26I GTCTC 1 cut(s) 391
AlwI GGATC 4 cut(s) 227, 257, 347, 789
AlwNI CAGNNNCTG 1 cut(s) 92
Ama87I CYCGRG 1 cut(s) 36
AoxI GGCC 4 cut(s) 107, 112, 684, 1113
ApaI GGGCCC 1 cut(s) 116
ApeKI GCWGC 1 cut(s) 323
ApoI RAATTY 4 cut(s) 372, 512, 557, 1396
Asp700I GAANNNNTTC 1 cut(s) 1085
AspS9I GGNCC 5 cut(s) 107, 112, 113, 685, 1114
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 2 cut(s) 245, 1427
AvaI CYCGRG 1 cut(s) 36
BaeGI GKGCMC 1 cut(s) 116
BaeI ACNNNNGTAYC 2 cut(s) 1152, 1185
BanII GRGCYC 3 cut(s) 43, 99, 116
BbsI GAAGAC 1 cut(s) 16
Bbv12I GWGCWC 2 cut(s) 43, 99
BbvI GCAGC 1 cut(s) 310
BccI CCATC 4 cut(s) 335, 863, 1447, 1558
BceAI ACGGC 2 cut(s) 972, 1557
BciT130I CCWGG 2 cut(s) 1118, 1591
BcnI CCSGG 1 cut(s) 111
BcoDI GTCTC 1 cut(s) 391
BfaI CTAG 3 cut(s) 117, 291, 1499
BfmI CTRYAG 2 cut(s) 1195, 1381
BfrI CTTAAG 2 cut(s) 230, 1328
BfuAI ACCTGC 1 cut(s) 515
BisI GCNGC 2 cut(s) 90, 324
BlpI GCTNAGC 1 cut(s) 93
BlsI GCNGC 2 cut(s) 91, 325
BmcAI AGTACT 1 cut(s) 643
Bme1390I CCNGG 3 cut(s) 111, 1118, 1591
BmeT110I CYCGRG 1 cut(s) 36
BmgT120I GGNCC 5 cut(s) 107, 112, 113, 685, 1114
BmiI GGNNCC 2 cut(s) 108, 114
BmrFI CCNGG 3 cut(s) 111, 1118, 1591
BmsI GCATC 5 cut(s) 466, 1034, 1312, 1323, 1522
BpiI GAAGAC 1 cut(s) 16
Bpu10I CCTNAGC 1 cut(s) 1431
Bpu1102I GCTNAGC 1 cut(s) 93
BpuMI CCSGG 1 cut(s) 111
BsaHI GRCGYC 1 cut(s) 169
BsaI GGTCTC 1 cut(s) 391
BsaJI CCNNGG 2 cut(s) 1116, 1590
BsaXI ACNNNNNCTCC 2 cut(s) 1088, 1118
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 1355, 1513
Bse1I ACTGG 1 cut(s) 1207
Bse3DI GCAATG 2 cut(s) 741, 909
BseBI CCWGG 2 cut(s) 1118, 1591
BseDI CCNNGG 2 cut(s) 1116, 1590
BseGI GGATG 3 cut(s) 1049, 1135, 1327
BseLI CCNNNNNNNGG 3 cut(s) 72, 1355, 1513
BseMI GCAATG 2 cut(s) 741, 909
BseMII CTCAG 2 cut(s) 84, 464
BseNI ACTGG 1 cut(s) 1207
BseRI GAGGAG 3 cut(s) 5, 8, 216
BseSI GKGCMC 1 cut(s) 116
BseXI GCAGC 1 cut(s) 310
BseYI CCCAGC 1 cut(s) 992
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 4 cut(s) 109, 114, 686, 1115
BsiEI CGRYCG 1 cut(s) 70
BsiHKAI GWGCWC 2 cut(s) 43, 99
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 1 cut(s) 110
BslI CCNNNNNNNGG 3 cut(s) 72, 1355, 1513
BsmAI GTCTC 1 cut(s) 391
BsnI GGCC 4 cut(s) 109, 114, 686, 1115
Bso31I GGTCTC 1 cut(s) 391
BsoBI CYCGRG 1 cut(s) 36
Bsp120I GGGCCC 1 cut(s) 112
Bsp1286I GDGCHC 3 cut(s) 43, 99, 116
Bsp143I GATC 5 cut(s) 219, 262, 352, 781, 924
Bsp1720I GCTNAGC 1 cut(s) 93
BspACI CCGC 2 cut(s) 61, 90
BspANI GGCC 4 cut(s) 109, 114, 686, 1115
BspCNI CTCAG 2 cut(s) 85, 463
BspHI TCATGA 3 cut(s) 138, 517, 540
BspLI GGNNCC 2 cut(s) 108, 114
BspMI ACCTGC 1 cut(s) 515
BspPI GGATC 4 cut(s) 227, 257, 347, 789
BspQI GCTCTTC 2 cut(s) 1028, 1133
BspTI CTTAAG 2 cut(s) 230, 1328
BspTNI GGTCTC 1 cut(s) 391
BsrBI CCGCTC 1 cut(s) 63
BsrDI GCAATG 2 cut(s) 741, 909
BsrI ACTGG 1 cut(s) 1207
BssECI CCNNGG 2 cut(s) 1116, 1590
BssMI GATC 5 cut(s) 219, 262, 352, 781, 924
BssNI GRCGYC 1 cut(s) 169
Bst2UI CCWGG 2 cut(s) 1118, 1591
Bst4CI ACNGT 6 cut(s) 71, 127, 693, 1165, 1196, 1270
Bst6I CTCTTC 4 cut(s) 707, 1028, 1133, 1665
BstACI GRCGYC 1 cut(s) 169
BstAFI CTTAAG 2 cut(s) 230, 1328
BstAPI GCANNNNNTGC 2 cut(s) 943, 1044
BstC8I GCNNGC 2 cut(s) 167, 1638
BstDEI CTNAG 5 cut(s) 93, 146, 450, 825, 1431
BstENI CCTNNNNNAGG 1 cut(s) 1511
BstF5I GGATG 3 cut(s) 1049, 1135, 1327
BstKTI GATC 5 cut(s) 222, 265, 355, 784, 927
BstMAI GTCTC 1 cut(s) 391
BstMBI GATC 5 cut(s) 219, 262, 352, 781, 924
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 6 cut(s) 38, 425, 476, 943, 1044, 1541
BstNI CCWGG 2 cut(s) 1118, 1591
BstNSI RCATGY 3 cut(s) 365, 741, 913
BstSCI CCNGG 3 cut(s) 109, 1116, 1589
BstSFI CTRYAG 2 cut(s) 1195, 1381
BstSLI GKGCMC 1 cut(s) 116
BstV1I GCAGC 1 cut(s) 310
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 4 cut(s) 219, 262, 352, 781
BstXI CCANNNNNNTGG 1 cut(s) 754
BstYI RGATCY 4 cut(s) 219, 262, 352, 781
BsuRI GGCC 4 cut(s) 109, 114, 686, 1115
BtsCI GGATG 3 cut(s) 1049, 1135, 1327
BtsI GCAGTG 1 cut(s) 1577
BtsIMutI CAGTG 3 cut(s) 1192, 1266, 1577
BveI ACCTGC 1 cut(s) 515
Cac8I GCNNGC 2 cut(s) 167, 1638
CaiI CAGNNNCTG 1 cut(s) 92
CciI TCATGA 3 cut(s) 138, 517, 540
Cfr13I GGNCC 5 cut(s) 107, 112, 113, 685, 1114
CseI GACGC 1 cut(s) 158
Csp6I GTAC 3 cut(s) 642, 961, 1469
CviQI GTAC 3 cut(s) 642, 961, 1469
DdeI CTNAG 5 cut(s) 93, 146, 450, 825, 1431
DpnI GATC 5 cut(s) 221, 264, 354, 783, 926
DpnII GATC 5 cut(s) 219, 262, 352, 781, 924
Eam1104I CTCTTC 4 cut(s) 707, 1028, 1133, 1665
EarI CTCTTC 4 cut(s) 707, 1028, 1133, 1665
Ecl136II GAGCTC 2 cut(s) 41, 97
Eco24I GRGCYC 3 cut(s) 43, 99, 116
Eco31I GGTCTC 1 cut(s) 391
Eco53kI GAGCTC 2 cut(s) 41, 97
Eco57I CTGAAG 1 cut(s) 69
Eco88I CYCGRG 1 cut(s) 36
EcoICRI GAGCTC 2 cut(s) 41, 97
EcoNI CCTNNNNNAGG 1 cut(s) 1511
EcoO109I RGGNCCY 1 cut(s) 113
EcoRI GAATTC 1 cut(s) 1396
EcoRII CCWGG 2 cut(s) 1116, 1589
EcoT22I ATGCAT 4 cut(s) 570, 638, 705, 743
EcoT38I GRGCYC 3 cut(s) 43, 99, 116
FblI GTMKAC 1 cut(s) 1170
Fnu4HI GCNGC 2 cut(s) 90, 324
FokI GGATG 3 cut(s) 1056, 1142, 1334
FriOI GRGCYC 3 cut(s) 43, 99, 116
Fsp4HI GCNGC 2 cut(s) 90, 324
FspBI CTAG 3 cut(s) 117, 291, 1499
GluI GCNGC 2 cut(s) 90, 324
GsaI CCCAGC 1 cut(s) 996
HaeIII GGCC 4 cut(s) 109, 114, 686, 1115
HapII CCGG 1 cut(s) 110
HgaI GACGC 1 cut(s) 158
Hin1I GRCGYC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 9 cut(s) 12, 142, 670, 715, 788, 1086, 1569, 1586, 1607
HpaII CCGG 1 cut(s) 110
HphI GGTGA 2 cut(s) 245, 1427
Hpy166II GTNNAC 1 cut(s) 1171
Hpy188I TCNGA 5 cut(s) 67, 391, 1077, 1368, 1410
Hpy188III TCNNGA 8 cut(s) 36, 139, 272, 518, 541, 614, 785, 928
Hpy8I GTNNAC 1 cut(s) 1171
Hpy99I CGWCG 2 cut(s) 75, 174
HpyAV CCTTC 5 cut(s) 201, 633, 925, 970, 1245
HpyCH4III ACNGT 6 cut(s) 71, 127, 693, 1165, 1196, 1270
HpyCH4IV ACGT 2 cut(s) 343, 959
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 425, 476, 943, 1044, 1541
HpyF3I CTNAG 5 cut(s) 93, 146, 450, 825, 1431
HpySE526I ACGT 2 cut(s) 343, 959
Hsp92I GRCGYC 1 cut(s) 169
Kzo9I GATC 5 cut(s) 219, 262, 352, 781, 924
LguI GCTCTTC 2 cut(s) 1028, 1133
LmnI GCTCC 4 cut(s) 68, 391, 934, 1120
Lsp1109I GCAGC 1 cut(s) 310
LweI GCATC 5 cut(s) 466, 1034, 1312, 1323, 1522
MaeI CTAG 3 cut(s) 117, 291, 1499
MaeII ACGT 2 cut(s) 343, 959
MaeIII GTNAC 2 cut(s) 1270, 1415
MalI GATC 5 cut(s) 221, 264, 354, 783, 926
MbiI CCGCTC 1 cut(s) 63
MboI GATC 5 cut(s) 219, 262, 352, 781, 924
MboII GAAGA 8 cut(s) 21, 721, 724, 820, 1027, 1045, 1150, 1682
MflI RGATCY 4 cut(s) 219, 262, 352, 781
MhlI GDGCHC 3 cut(s) 43, 99, 116
MlyI GAGTC 2 cut(s) 6, 724
MmeI TCCRAC 2 cut(s) 90, 348
Mph1103I ATGCAT 4 cut(s) 570, 638, 705, 743
MroXI GAANNNNTTC 1 cut(s) 1085
MslI CAYNNNNRTG 2 cut(s) 414, 971
MspA1I CMGCKG 1 cut(s) 92
MspCI CTTAAG 2 cut(s) 230, 1328
MspI CCGG 1 cut(s) 110
MspR9I CCNGG 3 cut(s) 111, 1118, 1591
MvaI CCWGG 2 cut(s) 1118, 1591
MwoI GCNNNNNNNGC 6 cut(s) 38, 425, 476, 943, 1044, 1541
NciI CCSGG 1 cut(s) 111
NdeII GATC 5 cut(s) 219, 262, 352, 781, 924
NlaIV GGNNCC 2 cut(s) 108, 114
NmuCI GTSAC 2 cut(s) 1270, 1415
NsiI ATGCAT 4 cut(s) 570, 638, 705, 743
NspI RCATGY 3 cut(s) 365, 741, 913
PaeR7I CTCGAG 1 cut(s) 36
PagI TCATGA 3 cut(s) 138, 517, 540
PciI ACATGT 1 cut(s) 909
PciSI GCTCTTC 2 cut(s) 1028, 1133
PdmI GAANNNNTTC 1 cut(s) 1085
PfeI GAWTC 7 cut(s) 142, 670, 788, 1086, 1569, 1586, 1607
PkrI GCNGC 2 cut(s) 91, 325
PleI GAGTC 2 cut(s) 6, 723
PpsI GAGTC 2 cut(s) 6, 723
PscI ACATGT 1 cut(s) 909
Psp124BI GAGCTC 2 cut(s) 43, 99
Psp6I CCWGG 2 cut(s) 1116, 1589
PspFI CCCAGC 1 cut(s) 992
PspGI CCWGG 2 cut(s) 1116, 1589
PspN4I GGNNCC 2 cut(s) 108, 114
PspOMI GGGCCC 1 cut(s) 112
PspPI GGNCC 5 cut(s) 107, 112, 113, 685, 1114
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 4 cut(s) 219, 262, 352, 781
RsaI GTAC 3 cut(s) 643, 962, 1470
RsaNI GTAC 3 cut(s) 642, 961, 1469
RseI CAYNNNNRTG 2 cut(s) 414, 971
SacI GAGCTC 2 cut(s) 43, 99
SapI GCTCTTC 2 cut(s) 1028, 1133
SatI GCNGC 2 cut(s) 90, 324
Sau3AI GATC 5 cut(s) 219, 262, 352, 781, 924
Sau96I GGNCC 5 cut(s) 107, 112, 113, 685, 1114
ScaI AGTACT 1 cut(s) 643
SchI GAGTC 2 cut(s) 6, 724
ScrFI CCNGG 3 cut(s) 111, 1118, 1591
SduI GDGCHC 3 cut(s) 43, 99, 116
SfaNI GCATC 5 cut(s) 466, 1034, 1312, 1323, 1522
SfcI CTRYAG 2 cut(s) 1195, 1381
Sfr274I CTCGAG 1 cut(s) 36
SlaI CTCGAG 1 cut(s) 36
SmiMI CAYNNNNRTG 2 cut(s) 414, 971
SmlI CTYRAG 3 cut(s) 36, 230, 1328
SmoI CTYRAG 3 cut(s) 36, 230, 1328
SsiI CCGC 2 cut(s) 61, 90
SspI AATATT 1 cut(s) 942
SspMI CTAG 3 cut(s) 117, 291, 1499
SstI GAGCTC 2 cut(s) 43, 99
StyD4I CCNGG 3 cut(s) 109, 1116, 1589
TaaI ACNGT 6 cut(s) 71, 127, 693, 1165, 1196, 1270
TaiI ACGT 2 cut(s) 346, 962
TaqI TCGA 5 cut(s) 37, 194, 302, 929, 1653
TatI WGTACW 1 cut(s) 641
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 7 cut(s) 142, 670, 788, 1086, 1569, 1586, 1607
TscAI CASTG 3 cut(s) 1199, 1273, 1584
TseFI GTSAC 2 cut(s) 1270, 1415
TseI GCWGC 1 cut(s) 323
Tsp45I GTSAC 2 cut(s) 1270, 1415
TspGWI ACGGA 1 cut(s) 295
TspRI CASTG 3 cut(s) 1199, 1273, 1584
Vha464I CTTAAG 2 cut(s) 230, 1328
XagI CCTNNNNNAGG 1 cut(s) 1511
XapI RAATTY 4 cut(s) 372, 512, 557, 1396
XceI RCATGY 3 cut(s) 365, 741, 913
XcmI CCANNNNNNNNNTGG 1 cut(s) 1199
XhoI CTCGAG 1 cut(s) 36
XmiI GTMKAC 1 cut(s) 1170
XmnI GAANNNNTTC 1 cut(s) 1085
XspI CTAG 3 cut(s) 117, 291, 1499
ZrmI AGTACT 1 cut(s) 643
Zsp2I ATGCAT 4 cut(s) 570, 638, 705, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.