Rorug07G0302800

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002831
Physical Location & Seq
Reverse (-)
30119109 .. 30125001
5893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0302800.1

Sequence Viewer

Length: 1179 bp
ATGAGTATACAGAGCATGTTACGAAGAGGCCTCACTTTCAATCACCTCCCCAAATCCGTACGACCAAGTGGGCTGCGAGGAAGAAGGCCCTATGCTAACAATGCCGCCTCCGCCGCCGCCGCCGCCGAGGAACCAAACCCAGATGGCAACAAAGTCCGTGGAAGGAGGCTTAGCAACAAGGAGCTCAAAGCCCTTGTGGAATCATTTGTCCACGAGTACAGAGCAATGAATGCGGGGAAATTCCCTCCCTTTAAGTATACTCAGAGACAAGTGGGTGGCAGTTACTATAGAATTAGGGGGATCCTCCAGGAGCTACAGTACACAGCTAAGATGTCGCCCTCGTGTGGTGGGATTGAGCATTCGTTGGGACGAGAGGGAATAAGAGGAAGTGAGTCTTTGACTGAAGTCGGAGGTGTCATTGCTCCAACTGTTTCTGAGGCAGAAAATGACACAGATCTTAGTGTTGCCAGTGACGGGTGTTTAGAAGCTGAGGAAGAGTCCCAGACGTCCTCCTCAGTTGAGATCTTGTCCGAAGAACTTAGCACTCCAGGGAGAGGTTCTGATCTGGATGTCACACAAAGTAATATCTGGAAAGTGAATGCTGAGGGATCTTCATATATAGGCCCTGATATGGAGGAACTTAAGAAAACTCAATCTGTCTCTATTGCTCCAGGATGTGAGATGTTACAAGTAGGATTTGATGTTATTTCTCATTCACATGACAAATCTAAGGATGGTAAAAGGGAGGAAGCTCAGGCTGATCGACATGATTATGTCTCTAAAGAAAAGCATCTACATATAGGGGAAACTAATACAGCCTCTCCAAGTTCTGATGATACGGAGGATATTACTAAACCGGATCTTGATGATTCAGAGTGTAAAATGAAGCAACATGATGAGACCCCTCAGGCTGATCGACTTGATTCCATTGCTGCAGAAAAACATCTACCAATAGAGGAAACTAATAGAGTCTCTTCAAGTTCTGATGATACAGAGGATGTTACTAAACCAGATCTTGATGATTCAAAGTCTATAGTGGAGCAACATGAAGAGATTCCTCAGGCAGACAAACTATCCAAGCTGGCTGATGATGCAGAACCTCCAAAAAGATCAACAACTCTATGGGGTGCTATGAAATCGTTTGCGGATGGAGTTATCAGTATTTTCAGACAACAGTAA

Protein Analysis

392

Amino Acids

42.96

Weight (kDa)

4.98

Isoelectric Point (pI)

58.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_AT3G52170 PF25896 59 - 107 3.6e-22 Helix-turn-helix domain in AT3G52170-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 509
AccI GTMKAC 2 cut(s) 7, 257
AciI CCGC 8 cut(s) 105, 111, 114, 117, 120, 123, 233, 1145
AclWI GGATC 4 cut(s) 295, 308, 616, 867
AcsI RAATTY 1 cut(s) 239
AcuI CTGAAG 1 cut(s) 423
AcyI GRCGYC 1 cut(s) 506
AfaI GTAC 3 cut(s) 60, 218, 320
AfiI CCNNNNNNNGG 4 cut(s) 344, 474, 554, 631
AflII CTTAAG 1 cut(s) 641
AgsI TTSAA 3 cut(s) 40, 978, 1026
AjnI CCWGG 3 cut(s) 306, 547, 670
AluBI AGCT 6 cut(s) 184, 313, 326, 488, 752, 1081
AluI AGCT 6 cut(s) 184, 313, 326, 488, 752, 1081
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 5 cut(s) 259, 664, 781, 893, 976
AlwI GGATC 4 cut(s) 295, 308, 616, 867
AoxI GGCC 3 cut(s) 28, 86, 622
ApeKI GCWGC 2 cut(s) 73, 932
ApoI RAATTY 1 cut(s) 239
Asp700I GAANNNNTTC 1 cut(s) 1053
AspS9I GGNCC 2 cut(s) 87, 623
AsuHPI GGTGA 1 cut(s) 35
AxyI CCTNAGG 2 cut(s) 906, 1059
BamHI GGATCC 1 cut(s) 300
BanII GRGCYC 1 cut(s) 186
BauI CACGAG 2 cut(s) 212, 340
Bbv12I GWGCWC 1 cut(s) 186
BbvCI CCTCAGC 2 cut(s) 489, 603
BbvI GCAGC 2 cut(s) 60, 919
BccI CCATC 3 cut(s) 137, 728, 1142
BciT130I CCWGG 3 cut(s) 308, 549, 672
BcoDI GTCTC 5 cut(s) 259, 664, 781, 893, 976
BfmI CTRYAG 4 cut(s) 286, 314, 933, 1032
BfrI CTTAAG 1 cut(s) 641
BglII AGATCT 3 cut(s) 454, 522, 1012
BisI GCNGC 7 cut(s) 74, 105, 114, 117, 120, 123, 933
BlpI GCTNAGC 1 cut(s) 170
BlsI GCNGC 7 cut(s) 75, 106, 115, 118, 121, 124, 934
Bme1390I CCNGG 3 cut(s) 308, 549, 672
BmgT120I GGNCC 2 cut(s) 87, 623
BmiI GGNNCC 2 cut(s) 132, 302
BmrFI CCNGG 3 cut(s) 308, 549, 672
BmsI GCATC 2 cut(s) 799, 1081
BoxI GACNNNNGTC 1 cut(s) 404
BpmI CTGGAG 3 cut(s) 290, 531, 654
Bpu10I CCTNAGC 3 cut(s) 489, 603, 753
Bpu1102I GCTNAGC 1 cut(s) 170
BsaHI GRCGYC 1 cut(s) 506
BsaI GGTCTC 1 cut(s) 893
BsaJI CCNNGG 3 cut(s) 126, 157, 548
BsaWI WCCGGW 1 cut(s) 856
BsaXI ACNNNNNCTCC 4 cut(s) 302, 332, 805, 835
Bsc4I CCNNNNNNNGG 4 cut(s) 344, 474, 554, 631
Bse1I ACTGG 1 cut(s) 468
Bse21I CCTNAGG 2 cut(s) 906, 1059
Bse3DI GCAATG 3 cut(s) 231, 417, 927
BseBI CCWGG 3 cut(s) 308, 549, 672
BseDI CCNNGG 3 cut(s) 126, 157, 548
BseGI GGATG 5 cut(s) 574, 680, 739, 1003, 1153
BseLI CCNNNNNNNGG 4 cut(s) 344, 474, 554, 631
BseMI GCAATG 3 cut(s) 231, 417, 927
BseMII CTCAG 8 cut(s) 275, 426, 480, 528, 594, 767, 920, 1073
BseNI ACTGG 1 cut(s) 468
BseRI GAGGAG 1 cut(s) 502
BseXI GCAGC 2 cut(s) 60, 919
BshFI GGCC 3 cut(s) 30, 88, 624
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 1 cut(s) 857
BsiWI CGTACG 1 cut(s) 58
BslFI GGGAC 2 cut(s) 381, 484
BslI CCNNNNNNNGG 4 cut(s) 344, 474, 554, 631
BsmAI GTCTC 5 cut(s) 259, 664, 781, 893, 976
BsmFI GGGAC 2 cut(s) 381, 484
BsmI GAATGC 3 cut(s) 235, 358, 604
BsnI GGCC 3 cut(s) 30, 88, 624
Bso31I GGTCTC 1 cut(s) 893
Bsp1286I GDGCHC 1 cut(s) 186
Bsp1720I GCTNAGC 1 cut(s) 170
BspACI CCGC 8 cut(s) 105, 111, 114, 117, 120, 123, 233, 1145
BspANI GGCC 3 cut(s) 30, 88, 624
BspCNI CTCAG 8 cut(s) 274, 427, 481, 527, 595, 766, 919, 1072
BspLI GGNNCC 2 cut(s) 132, 302
BspMAI CTGCAG 1 cut(s) 937
BspPI GGATC 4 cut(s) 295, 308, 616, 867
BspTI CTTAAG 1 cut(s) 641
BspTNI GGTCTC 1 cut(s) 893
BsrDI GCAATG 3 cut(s) 231, 417, 927
BsrI ACTGG 1 cut(s) 468
BssECI CCNNGG 3 cut(s) 126, 157, 548
BssNAI GTATAC 2 cut(s) 8, 258
BssNI GRCGYC 1 cut(s) 506
BssSI CACGAG 2 cut(s) 212, 340
Bst1107I GTATAC 2 cut(s) 8, 258
Bst2BI CACGAG 2 cut(s) 212, 340
Bst2UI CCWGG 3 cut(s) 308, 549, 672
Bst4CI ACNGT 3 cut(s) 318, 430, 1176
Bst6I CTCTTC 4 cut(s) 19, 489, 979, 1044
BstACI GRCGYC 1 cut(s) 506
BstAFI CTTAAG 1 cut(s) 641
BstAPI GCANNNNNTGC 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 1083
BstDSI CCRYGG 1 cut(s) 157
BstF5I GGATG 5 cut(s) 574, 680, 739, 1003, 1153
BstMAI GTCTC 5 cut(s) 259, 664, 781, 893, 976
BstMWI GCNNNNNNNGC 7 cut(s) 101, 110, 113, 119, 122, 230, 1091
BstNI CCWGG 3 cut(s) 308, 549, 672
BstNSI RCATGY 1 cut(s) 19
BstPAI GACNNNNGTC 1 cut(s) 404
BstSCI CCNGG 3 cut(s) 306, 547, 670
BstSFI CTRYAG 4 cut(s) 286, 314, 933, 1032
BstV1I GCAGC 2 cut(s) 60, 919
BstX2I RGATCY 6 cut(s) 300, 454, 522, 608, 859, 1012
BstYI RGATCY 6 cut(s) 300, 454, 522, 608, 859, 1012
BstZ17I GTATAC 2 cut(s) 8, 258
Bsu36I CCTNAGG 2 cut(s) 906, 1059
BsuRI GGCC 3 cut(s) 30, 88, 624
BtgI CCRYGG 1 cut(s) 157
BtsCI GGATG 5 cut(s) 574, 680, 739, 1003, 1153
BtsIMutI CAGTG 1 cut(s) 475
Cac8I GCNNGC 1 cut(s) 1083
Cfr13I GGNCC 2 cut(s) 87, 623
Csp6I GTAC 3 cut(s) 59, 217, 319
CspCI CAANNNNNGTGG 2 cut(s) 139, 174
CviAII CATG 5 cut(s) 16, 719, 767, 893, 1046
CviQI GTAC 3 cut(s) 59, 217, 319
Eam1104I CTCTTC 4 cut(s) 19, 489, 979, 1044
EarI CTCTTC 4 cut(s) 19, 489, 979, 1044
EciI GGCGGA 1 cut(s) 100
Ecl136II GAGCTC 1 cut(s) 184
Eco147I AGGCCT 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 186
Eco31I GGTCTC 1 cut(s) 893
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 423
Eco81I CCTNAGG 2 cut(s) 906, 1059
EcoICRI GAGCTC 1 cut(s) 184
EcoO109I RGGNCCY 2 cut(s) 87, 623
EcoRII CCWGG 3 cut(s) 306, 547, 670
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 5 cut(s) 19, 722, 770, 896, 1049
FalI AAGNNNNNCTT 2 cut(s) 379, 411
FaqI GGGAC 2 cut(s) 381, 484
FatI CATG 5 cut(s) 15, 718, 766, 892, 1045
FauI CCCGC 1 cut(s) 226
FblI GTMKAC 2 cut(s) 7, 257
Fnu4HI GCNGC 7 cut(s) 74, 105, 114, 117, 120, 123, 933
FokI GGATG 5 cut(s) 581, 687, 746, 1010, 1160
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 7 cut(s) 74, 105, 114, 117, 120, 123, 933
GluI GCNGC 7 cut(s) 74, 105, 114, 117, 120, 123, 933
GsuI CTGGAG 3 cut(s) 290, 531, 654
HaeIII GGCC 3 cut(s) 30, 88, 624
HapII CCGG 1 cut(s) 857
Hin1I GRCGYC 1 cut(s) 506
Hin1II CATG 5 cut(s) 19, 722, 770, 896, 1049
HinfI GANTC 8 cut(s) 200, 392, 497, 869, 923, 969, 1022, 1054
HpaII CCGG 1 cut(s) 857
HphI GGTGA 1 cut(s) 35
Hpy166II GTNNAC 4 cut(s) 8, 211, 258, 321
Hpy188I TCNGA 9 cut(s) 264, 410, 436, 532, 562, 832, 874, 985, 1169
Hpy188III TCNNGA 4 cut(s) 566, 589, 863, 1016
Hpy8I GTNNAC 4 cut(s) 8, 211, 258, 321
HpyAV CCTTC 2 cut(s) 78, 156
HpyCH4III ACNGT 3 cut(s) 318, 430, 1176
HpyCH4IV ACGT 1 cut(s) 506
HpyCH4V TGCA 2 cut(s) 935, 1094
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 110, 113, 119, 122, 230, 1091
HpySE526I ACGT 1 cut(s) 506
Hsp92I GRCGYC 1 cut(s) 506
Hsp92II CATG 5 cut(s) 19, 722, 770, 896, 1049
LmnI GCTCC 5 cut(s) 181, 310, 427, 673, 1039
Lsp1109I GCAGC 2 cut(s) 60, 919
LweI GCATC 2 cut(s) 799, 1081
MaeII ACGT 1 cut(s) 506
MaeIII GTNAC 6 cut(s) 18, 281, 470, 571, 684, 1000
MboII GAAGA 7 cut(s) 36, 93, 506, 545, 603, 966, 1061
MflI RGATCY 6 cut(s) 300, 454, 522, 608, 859, 1012
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 2 cut(s) 239, 291
MlyI GAGTC 3 cut(s) 401, 506, 978
MmeI TCCRAC 2 cut(s) 388, 449
MroXI GAANNNNTTC 1 cut(s) 1053
MseI TTAA 2 cut(s) 252, 642
MslI CAYNNNNRTG 2 cut(s) 717, 771
MspCI CTTAAG 1 cut(s) 641
MspI CCGG 1 cut(s) 857
MspR9I CCNGG 3 cut(s) 308, 549, 672
Mva1269I GAATGC 3 cut(s) 235, 358, 604
MvaI CCWGG 3 cut(s) 308, 549, 672
MwoI GCNNNNNNNGC 7 cut(s) 101, 110, 113, 119, 122, 230, 1091
NlaIII CATG 5 cut(s) 19, 722, 770, 896, 1049
NlaIV GGNNCC 2 cut(s) 132, 302
NmeAIII GCCGAG 1 cut(s) 151
NmuCI GTSAC 2 cut(s) 470, 571
NspI RCATGY 1 cut(s) 19
PceI AGGCCT 1 cut(s) 30
PctI GAATGC 3 cut(s) 235, 358, 604
PdmI GAANNNNTTC 1 cut(s) 1053
PfeI GAWTC 5 cut(s) 200, 869, 923, 1022, 1054
Pfl23II CGTACG 1 cut(s) 58
PfoI TCCNGGA 2 cut(s) 306, 670
PkrI GCNGC 7 cut(s) 75, 106, 115, 118, 121, 124, 934
PleI GAGTC 3 cut(s) 400, 505, 977
PpsI GAGTC 3 cut(s) 400, 505, 977
PshAI GACNNNNGTC 1 cut(s) 404
Psp124BI GAGCTC 1 cut(s) 186
Psp6I CCWGG 3 cut(s) 306, 547, 670
PspGI CCWGG 3 cut(s) 306, 547, 670
PspLI CGTACG 1 cut(s) 58
PspN4I GGNNCC 2 cut(s) 132, 302
PspPI GGNCC 2 cut(s) 87, 623
PstI CTGCAG 1 cut(s) 937
PsuI RGATCY 6 cut(s) 300, 454, 522, 608, 859, 1012
RsaI GTAC 3 cut(s) 60, 218, 320
RsaNI GTAC 3 cut(s) 59, 217, 319
RseI CAYNNNNRTG 2 cut(s) 717, 771
SacI GAGCTC 1 cut(s) 186
SaqAI TTAA 2 cut(s) 252, 642
SatI GCNGC 7 cut(s) 74, 105, 114, 117, 120, 123, 933
Sau96I GGNCC 2 cut(s) 87, 623
SchI GAGTC 3 cut(s) 401, 506, 978
ScrFI CCNGG 3 cut(s) 308, 549, 672
SduI GDGCHC 1 cut(s) 186
SfaNI GCATC 2 cut(s) 799, 1081
SfcI CTRYAG 4 cut(s) 286, 314, 933, 1032
SmiMI CAYNNNNRTG 2 cut(s) 717, 771
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
Sse9I AATT 2 cut(s) 239, 291
SseBI AGGCCT 1 cut(s) 30
SsiI CCGC 8 cut(s) 105, 111, 114, 117, 120, 123, 233, 1145
SstI GAGCTC 1 cut(s) 186
StuI AGGCCT 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 306, 547, 670
TaaI ACNGT 3 cut(s) 318, 430, 1176
TaiI ACGT 1 cut(s) 509
TaqI TCGA 2 cut(s) 763, 916
TasI AATT 2 cut(s) 239, 291
TatI WGTACW 2 cut(s) 216, 318
TauI GCSGC 5 cut(s) 107, 116, 119, 122, 125
TfiI GAWTC 5 cut(s) 200, 869, 923, 1022, 1054
Tru1I TTAA 2 cut(s) 252, 642
Tru9I TTAA 2 cut(s) 252, 642
TscAI CASTG 1 cut(s) 475
TseFI GTSAC 2 cut(s) 470, 571
TseI GCWGC 2 cut(s) 73, 932
Tsp45I GTSAC 2 cut(s) 470, 571
TspDTI ATGAA 5 cut(s) 242, 603, 899, 1062, 1148
TspGWI ACGGA 3 cut(s) 46, 146, 854
TspRI CASTG 1 cut(s) 475
Vha464I CTTAAG 1 cut(s) 641
XapI RAATTY 1 cut(s) 239
XceI RCATGY 1 cut(s) 19
XmiI GTMKAC 2 cut(s) 7, 257
XmnI GAANNNNTTC 1 cut(s) 1053
ZraI GACGTC 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.