Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0002831

Physical Location & Seq

Genomic Coordinates Reverse (-)

46829194 .. 46830747

Gene Structure 1554 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G022020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1554 bp

ATGAATCTAAGGAGAGTTCATTTTCGTTGGTGCCGTTGGTTCGCTTCCTCTCCTTCAACAATCTCATCGTCTGTTTCATGGGTCTCTCCTCTTCAGTTATCAAAATTCAATGCTCACAAACCAGATCCCCCTCCCGAAACCACAATTACAAGGACAGAAGCTAGGAGGAGATCCAAATATATATCTCACGATGCTGCCATCAACTTAATCAAACGTGAGAGAGATCCACAGCATGCCTGGGAAATATTTAACATGGTTTCAGAGCAAAAGGGTTTCAACCACAATAATGCCACCTATGCAACTATCCTCAATAAGCTTGCTCAGAACAAGAAGTTCAAGGCTGTTGATGCAGTTCTCTATCAAATGAAGTACGATACCTGCAAATATCATGAGGGTATATTCCTTAATCTCATGAAGCATTTTTCAAAATCGTCTATGCATGAAAGAGTACTTGAAATGTTCTATGCTATCCAGCCAGTAGTTCGGGAAAAGCCATCTCTCAAATGCATTAGTACTTGTTTAAATCTACTGATTGAAGCGAATCAGGTTGATTTGGCCCAACAGTTTCTCATGCATTTGAAGAAGAGTCTTAACTTGAACCTGAATACATGCATTGTCAACATCTTGGTTAAGCACCATTGCACAAATGGGGATCTTGAATCTGCCTTCGAGGTTGTGAAGGAAATGAAAAAGTCTAAGCTTTCCTATCCTAATTTGATTACTTACTCGACTCTAATTGATGGTTTATGTCAAAGTGGAAGACTGACAGAAGCAATGGACCTGTTTGAGGAAATGATCTCAAAGGATCAGATATTGCCTGATGTGCTAACGTACAACATTTTGATTAATGGTTTTTGCCGTGGTGGGAAAGTTAATAGGGCAAGGAAGATATTGGATTTTATGAAGAGCAAAGGATGCAACCCAAATGTATTCAATTACTCAGCTCTGATGAATGGATTCTGTAAGGAGGAAAGATTTGAGGAGACCAAGGAGCTTTTGGATGAAATGAAGAGCTTTGGTATCAAACCAGATACAGTTGTCTACACTACTTTAATTGATTGCTACTGTAGGACTGGGAGAGTTGATGAAGTGATAGAGTTGCTCAAAGAGATGAAAGAAAGACGGTGCAAAGCTGATACTGTGACTTTCAATGTGATACTTGGAGGACTATGTAGAGAAGGCAGAATTGAGGATGCGCTTGAGATGCTGGACAAACTTCCTTATGAGGGTATCTATCTGAATAAGGGAAGCTACAGGATTGTGTTGAATTCCTTGTGTCAGACAGGCGAACTAAACAAAGCTAAAGAGTTGTTGATATTGATGATGGGTAGGGGGTTTGTACCACATTATGCAACTTCAAACGAGTTGCTAGTTAGCCTCTGTGAGGCTGGAATGGCAGATGATGCAACTATAGCTTTATTTGGATTGGTGGAGTTGGGTTTCAAACCACTGCCTGATTCCTGGGCTCTTTTTGTTGAATCAATTTGCAGAGAAAGAAAGCTGCTGCCTGCATTTGAACTGCTCGATGATTTAGTTAATGAAGAGTTTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

517

Amino Acids

59.31

Weight (kDa)

7.79

Isoelectric Point (pI)

39.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	196 - 247	1.2e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	202 - 251	3.2e-13	PPR repeat family
PPR_long	PF17177	202 - 269	1e-05	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	237 - 287	2.3e-17	PPR repeat family
PPR_1	PF12854	237 - 265	3.7e-13	PPR repeat
PPR	PF01535	240 - 268	2.1e-09	PPR repeat
PPR_long	PF17177	254 - 405	1e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	262 - 320	2.6e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	271 - 302	1.4e-12	PPR repeat
PPR_2	PF13041	273 - 322	2.2e-17	PPR repeat family
PPR	PF01535	277 - 306	6.5e-08	PPR repeat
PPR_1	PF12854	304 - 337	2.5e-10	PPR repeat
PPR_2	PF13041	308 - 353	8.8e-13	PPR repeat family
TPR_24	PF23276	312 - 402	6.1e-07	Fungal tetratrico peptide repeats
PPR	PF01535	313 - 341	2e-06	PPR repeat
PPR_3	PF13812	332 - 388	2.7e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	340 - 372	1.7e-12	PPR repeat
PPR_2	PF13041	343 - 392	3.2e-18	PPR repeat family
PPR	PF01535	346 - 376	2e-10	PPR repeat
PPR_1	PF12854	375 - 405	9.1e-10	PPR repeat
PPR_2	PF13041	379 - 427	4.9e-11	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002831)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G18475
fragaria_vesca	FvH4_5g35730 FvH4_5g35730 FvH4_5g35730 FvH4_5g35730 FvH4_5g35730
prunus_persica	Prupe.1G528200_v2.0.a1
pyrus_communis	pycom08g16680
rosa_chinensis	RchiOBHm_Chr4g0424581 RchiOBHm_Chr7g0237351
rosa_laevigata	RLG00000001010 RLG00000007454
rosa_multiflora	Rmu_co8091050.1_g000001 Rmu_sc0000281.1_g000013 Rmu_sc0000820.1_g000045 Rmu_sc0002603.1_g000021 Rmu_sc0002923.1_g000038 Rmu_sc0006438.1_g000010
rosa_roxburghii	Rroxscaffold_3G00224290 Rroxscaffold_3G00224300 Rroxscaffold_5G00366500
rosa_rugosa	Rorug04G0198000 Rorug07G0302800 Rorug07G0302800
rosa_samantha	Rh1CG247200 Rh1DG083900 Rh4AG254700 Rh4BG260200 Rh4CG271700 Rh4DG254900 Rh5AG212800 Rh6DG464200 Rh7AG460000 Rh7BG429900 Rh7CG477300 Rh7DG446600
rosa_wichuraiana	Rw1G023340 Rw4G022020 Rw7G038100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	386
AccB1I	GGYRCC	1 cut(s)	30
AccI	GTMKAC	1 cut(s)	1041
AclWI	GGATC	5 cut(s)	119, 165, 218, 660, 813
AcsI	RAATTY	2 cut(s)	104, 1267
AcuI	CTGAAG	1 cut(s)	77
AfaI	GTAC	5 cut(s)	371, 450, 514, 833, 1341
AfiI	CCNNNNNNNGG	3 cut(s)	787, 1226, 1384
AjnI	CCWGG	2 cut(s)	236, 1460
Alw26I	GTCTC	2 cut(s)	88, 977
AlwI	GGATC	5 cut(s)	119, 165, 218, 660, 813
AoxI	GGCC	1 cut(s)	555
ApeKI	GCWGC	3 cut(s)	194, 1501, 1504
ApoI	RAATTY	2 cut(s)	104, 1267
AseI	ATTAAT	1 cut(s)	846
Asp700I	GAANNNNTTC	1 cut(s)	956
AspLEI	GCGC	1 cut(s)	1198
AspS9I	GGNCC	2 cut(s)	556, 778
AvaII	GGWCC	1 cut(s)	778
BaeI	ACNNNNGTAYC	2 cut(s)	1023, 1056
BanI	GGYRCC	1 cut(s)	30
BanII	GRGCYC	1 cut(s)	1468
BbsI	GAAGAC	1 cut(s)	766
BbvI	GCAGC	3 cut(s)	181, 1488, 1491
BccI	CCATC	4 cut(s)	206, 502, 734, 1318
BceAI	ACGGC	2 cut(s)	18, 843
BciT130I	CCWGG	2 cut(s)	238, 1462
BcoDI	GTCTC	2 cut(s)	88, 977
BfaI	CTAG	2 cut(s)	162, 1370
BfmI	CTRYAG	3 cut(s)	1066, 1252, 1410
BfuAI	ACCTGC	1 cut(s)	386
BisI	GCNGC	3 cut(s)	195, 1502, 1505
BlsI	GCNGC	3 cut(s)	196, 1503, 1506
BmcAI	AGTACT	2 cut(s)	450, 514
Bme1390I	CCNGG	2 cut(s)	238, 1462
Bme18I	GGWCC	1 cut(s)	778
BmgT120I	GGNCC	2 cut(s)	556, 778
BmiI	GGNNCC	1 cut(s)	32
BmrFI	CCNGG	2 cut(s)	238, 1462
BmrI	ACTGGG	1 cut(s)	1083
BmsI	GCATC	6 cut(s)	181, 337, 905, 1183, 1194, 1393
BmuI	ACTGGG	1 cut(s)	1083
BpiI	GAAGAC	1 cut(s)	766
BpuEI	CTTGAG	1 cut(s)	1220
BsaI	GGTCTC	2 cut(s)	88, 977
BsaJI	CCNNGG	4 cut(s)	237, 859, 987, 1461
Bsc4I	CCNNNNNNNGG	3 cut(s)	787, 1226, 1384
Bse1I	ACTGG	2 cut(s)	476, 1078
Bse3DI	GCAATG	2 cut(s)	637, 780
BseBI	CCWGG	2 cut(s)	238, 1462
BseDI	CCNNGG	4 cut(s)	237, 859, 987, 1461
BseGI	GGATG	3 cut(s)	920, 1006, 1198
BseLI	CCNNNNNNNGG	3 cut(s)	787, 1226, 1384
BseMI	GCAATG	2 cut(s)	637, 780
BseMII	CTCAG	2 cut(s)	335, 954
BseNI	ACTGG	2 cut(s)	476, 1078
BseRI	GAGGAG	3 cut(s)	78, 181, 995
BseXI	GCAGC	3 cut(s)	181, 1488, 1491
BshFI	GGCC	1 cut(s)	557
BshNI	GGYRCC	1 cut(s)	30
BslI	CCNNNNNNNGG	3 cut(s)	787, 1226, 1384
BsmAI	GTCTC	2 cut(s)	88, 977
BsnI	GGCC	1 cut(s)	557
Bso31I	GGTCTC	2 cut(s)	88, 977
Bsp1286I	GDGCHC	1 cut(s)	1468
Bsp143I	GATC	6 cut(s)	124, 170, 223, 652, 795, 805
BspANI	GGCC	1 cut(s)	557
BspCNI	CTCAG	2 cut(s)	334, 953
BspHI	TCATGA	2 cut(s)	388, 411
BspLI	GGNNCC	1 cut(s)	32
BspMI	ACCTGC	1 cut(s)	386
BspPI	GGATC	5 cut(s)	119, 165, 218, 660, 813
BspQI	GCTCTTC	2 cut(s)	899, 1004
BspT107I	GGYRCC	1 cut(s)	30
BspTNI	GGTCTC	2 cut(s)	88, 977
BsrDI	GCAATG	2 cut(s)	637, 780
BsrI	ACTGG	2 cut(s)	476, 1078
BssECI	CCNNGG	4 cut(s)	237, 859, 987, 1461
BssMI	GATC	6 cut(s)	124, 170, 223, 652, 795, 805
BssT1I	CCWWGG	1 cut(s)	987
Bst2UI	CCWGG	2 cut(s)	238, 1462
Bst4CI	ACNGT	5 cut(s)	564, 1036, 1067, 1125, 1141
Bst6I	CTCTTC	5 cut(s)	96, 578, 899, 1004, 1536
BstAPI	GCANNNNNTGC	2 cut(s)	915, 1403
BstC8I	GCNNGC	3 cut(s)	234, 318, 1509
BstDEI	CTNAG	4 cut(s)	8, 321, 696, 940
BstDSI	CCRYGG	1 cut(s)	859
BstENI	CCTNNNNNAGG	2 cut(s)	785, 1382
BstF5I	GGATG	3 cut(s)	920, 1006, 1198
BstHHI	GCGC	1 cut(s)	1198
BstKTI	GATC	6 cut(s)	127, 173, 226, 655, 798, 808
BstMAI	GTCTC	2 cut(s)	88, 977
BstMBI	GATC	6 cut(s)	124, 170, 223, 652, 795, 805
BstMWI	GCNNNNNNNGC	8 cut(s)	296, 347, 823, 915, 1204, 1394, 1403, 1412
BstNI	CCWGG	2 cut(s)	238, 1462
BstNSI	RCATGY	2 cut(s)	236, 612
BstSCI	CCNGG	2 cut(s)	236, 1460
BstSFI	CTRYAG	3 cut(s)	1066, 1252, 1410
BstV1I	GCAGC	3 cut(s)	181, 1488, 1491
BstV2I	GAAGAC	1 cut(s)	766
BstX2I	RGATCY	4 cut(s)	124, 170, 223, 652
BstYI	RGATCY	4 cut(s)	124, 170, 223, 652
BsuRI	GGCC	1 cut(s)	557
BtgI	CCRYGG	1 cut(s)	859
BtsCI	GGATG	3 cut(s)	920, 1006, 1198
BtsI	GCAGTG	1 cut(s)	1448
BtsIMutI	CAGTG	1 cut(s)	1448
BveI	ACCTGC	1 cut(s)	386
Cac8I	GCNNGC	3 cut(s)	234, 318, 1509
CciI	TCATGA	2 cut(s)	388, 411
CfoI	GCGC	1 cut(s)	1198
Cfr13I	GGNCC	2 cut(s)	556, 778
Csp6I	GTAC	5 cut(s)	370, 449, 513, 832, 1340
CviAII	CATG	8 cut(s)	78, 233, 253, 389, 412, 440, 571, 609
CviQI	GTAC	5 cut(s)	370, 449, 513, 832, 1340
DdeI	CTNAG	4 cut(s)	8, 321, 696, 940
DpnI	GATC	6 cut(s)	126, 172, 225, 654, 797, 807
DpnII	GATC	6 cut(s)	124, 170, 223, 652, 795, 805
DraI	TTTAAA	1 cut(s)	522
Eam1104I	CTCTTC	5 cut(s)	96, 578, 899, 1004, 1536
EarI	CTCTTC	5 cut(s)	96, 578, 899, 1004, 1536
Eco130I	CCWWGG	1 cut(s)	987
Eco24I	GRGCYC	1 cut(s)	1468
Eco31I	GGTCTC	2 cut(s)	88, 977
Eco47I	GGWCC	1 cut(s)	778
Eco57I	CTGAAG	1 cut(s)	77
EcoNI	CCTNNNNNAGG	2 cut(s)	785, 1382
EcoRI	GAATTC	1 cut(s)	1267
EcoRII	CCWGG	2 cut(s)	236, 1460
EcoT14I	CCWWGG	1 cut(s)	987
EcoT22I	ATGCAT	4 cut(s)	441, 509, 576, 614
EcoT38I	GRGCYC	1 cut(s)	1468
ErhI	CCWWGG	1 cut(s)	987
FaeI	CATG	8 cut(s)	81, 236, 256, 392, 415, 443, 574, 612
FatI	CATG	8 cut(s)	77, 232, 252, 388, 411, 439, 570, 608
FblI	GTMKAC	1 cut(s)	1041
Fnu4HI	GCNGC	3 cut(s)	195, 1502, 1505
FokI	GGATG	3 cut(s)	927, 1013, 1205
FriOI	GRGCYC	1 cut(s)	1468
Fsp4HI	GCNGC	3 cut(s)	195, 1502, 1505
FspBI	CTAG	2 cut(s)	162, 1370
GlaI	GCGC	1 cut(s)	1197
GluI	GCNGC	3 cut(s)	195, 1502, 1505
HaeIII	GGCC	1 cut(s)	557
HhaI	GCGC	1 cut(s)	1198
Hin1II	CATG	8 cut(s)	81, 236, 256, 392, 415, 443, 574, 612
Hin6I	GCGC	1 cut(s)	1196
HinP1I	GCGC	1 cut(s)	1196
HincII	GTYRAC	1 cut(s)	619
HindII	GTYRAC	1 cut(s)	619
HindIII	AAGCTT	2 cut(s)	314, 698
HinfI	GANTC	8 cut(s)	4, 541, 586, 659, 730, 957, 1457, 1478
Hpy166II	GTNNAC	2 cut(s)	619, 1042
Hpy188I	TCNGA	6 cut(s)	262, 324, 810, 948, 1239, 1281
Hpy188III	TCNNGA	6 cut(s)	134, 188, 389, 412, 485, 656
Hpy8I	GTNNAC	2 cut(s)	619, 1042
HpyAV	CCTTC	4 cut(s)	63, 673, 676, 1172
HpyCH4III	ACNGT	5 cut(s)	564, 1036, 1067, 1125, 1141
HpyCH4IV	ACGT	2 cut(s)	214, 830
HpyF10VI	GCNNNNNNNGC	8 cut(s)	296, 347, 823, 915, 1204, 1394, 1403, 1412
HpyF3I	CTNAG	4 cut(s)	8, 321, 696, 940
HpySE526I	ACGT	2 cut(s)	214, 830
Hsp92II	CATG	8 cut(s)	81, 236, 256, 392, 415, 443, 574, 612
HspAI	GCGC	1 cut(s)	1196
Kzo9I	GATC	6 cut(s)	124, 170, 223, 652, 795, 805
LguI	GCTCTTC	2 cut(s)	899, 1004
LmnI	GCTCC	1 cut(s)	991
Lsp1109I	GCAGC	3 cut(s)	181, 1488, 1491
LweI	GCATC	6 cut(s)	181, 337, 905, 1183, 1194, 1393
MaeI	CTAG	2 cut(s)	162, 1370
MaeII	ACGT	2 cut(s)	214, 830
MaeIII	GTNAC	1 cut(s)	1141
MalI	GATC	6 cut(s)	126, 172, 225, 654, 797, 807
MboI	GATC	6 cut(s)	124, 170, 223, 652, 795, 805
MboII	GAAGA	8 cut(s)	83, 592, 595, 771, 898, 916, 1021, 1553
MflI	RGATCY	4 cut(s)	124, 170, 223, 652
MhlI	GDGCHC	1 cut(s)	1468
MluCI	AATT	9 cut(s)	104, 144, 712, 735, 934, 1053, 1185, 1267, 1482
MlyI	GAGTC	2 cut(s)	595, 724
Mph1103I	ATGCAT	4 cut(s)	441, 509, 576, 614
MroXI	GAANNNNTTC	1 cut(s)	956
MslI	CAYNNNNRTG	1 cut(s)	285
MspR9I	CCNGG	2 cut(s)	238, 1462
MvaI	CCWGG	2 cut(s)	238, 1462
MwoI	GCNNNNNNNGC	8 cut(s)	296, 347, 823, 915, 1204, 1394, 1403, 1412
NdeII	GATC	6 cut(s)	124, 170, 223, 652, 795, 805
NlaIII	CATG	8 cut(s)	81, 236, 256, 392, 415, 443, 574, 612
NlaIV	GGNNCC	1 cut(s)	32
NmuCI	GTSAC	1 cut(s)	1141
NsiI	ATGCAT	4 cut(s)	441, 509, 576, 614
NspI	RCATGY	2 cut(s)	236, 612
PaeI	GCATGC	1 cut(s)	236
PagI	TCATGA	2 cut(s)	388, 411
PciSI	GCTCTTC	2 cut(s)	899, 1004
PcsI	WCGNNNNNNNCGW	1 cut(s)	31
PdmI	GAANNNNTTC	1 cut(s)	956
PfeI	GAWTC	6 cut(s)	4, 541, 659, 957, 1457, 1478
PkrI	GCNGC	3 cut(s)	196, 1503, 1506
PleI	GAGTC	2 cut(s)	594, 724
PpsI	GAGTC	2 cut(s)	594, 724
PshBI	ATTAAT	1 cut(s)	846
Psp6I	CCWGG	2 cut(s)	236, 1460
PspGI	CCWGG	2 cut(s)	236, 1460
PspN4I	GGNNCC	1 cut(s)	32
PspPI	GGNCC	2 cut(s)	556, 778
PsuI	RGATCY	4 cut(s)	124, 170, 223, 652
RsaI	GTAC	5 cut(s)	371, 450, 514, 833, 1341
RsaNI	GTAC	5 cut(s)	370, 449, 513, 832, 1340
RseI	CAYNNNNRTG	1 cut(s)	285
SapI	GCTCTTC	2 cut(s)	899, 1004
SatI	GCNGC	3 cut(s)	195, 1502, 1505
Sau3AI	GATC	6 cut(s)	124, 170, 223, 652, 795, 805
Sau96I	GGNCC	2 cut(s)	556, 778
ScaI	AGTACT	2 cut(s)	450, 514
SchI	GAGTC	2 cut(s)	595, 724
ScrFI	CCNGG	2 cut(s)	238, 1462
SduI	GDGCHC	1 cut(s)	1468
SfaNI	GCATC	6 cut(s)	181, 337, 905, 1183, 1194, 1393
SfcI	CTRYAG	3 cut(s)	1066, 1252, 1410
SinI	GGWCC	1 cut(s)	778
SmiMI	CAYNNNNRTG	1 cut(s)	285
SmlI	CTYRAG	1 cut(s)	1199
SmoI	CTYRAG	1 cut(s)	1199
SphI	GCATGC	1 cut(s)	236
Sse9I	AATT	9 cut(s)	104, 144, 712, 735, 934, 1053, 1185, 1267, 1482
SspI	AATATT	1 cut(s)	246
SspMI	CTAG	2 cut(s)	162, 1370
StyD4I	CCNGG	2 cut(s)	236, 1460
StyI	CCWWGG	1 cut(s)	987
TaaI	ACNGT	5 cut(s)	564, 1036, 1067, 1125, 1141
TaiI	ACGT	2 cut(s)	217, 833
TaqI	TCGA	3 cut(s)	669, 728, 1524
TasI	AATT	9 cut(s)	104, 144, 712, 735, 934, 1053, 1185, 1267, 1482
TatI	WGTACW	2 cut(s)	448, 512
TfiI	GAWTC	6 cut(s)	4, 541, 659, 957, 1457, 1478
TscAI	CASTG	1 cut(s)	1455
TseFI	GTSAC	1 cut(s)	1141
TseI	GCWGC	3 cut(s)	194, 1501, 1504
Tsp45I	GTSAC	1 cut(s)	1141
TspRI	CASTG	1 cut(s)	1455
VpaK11BI	GGWCC	1 cut(s)	778
VspI	ATTAAT	1 cut(s)	846
XagI	CCTNNNNNAGG	2 cut(s)	785, 1382
XapI	RAATTY	2 cut(s)	104, 1267
XceI	RCATGY	2 cut(s)	236, 612
XcmI	CCANNNNNNNNNTGG	3 cut(s)	234, 644, 994
XmiI	GTMKAC	1 cut(s)	1041
XmnI	GAANNNNTTC	1 cut(s)	956
XspI	CTAG	2 cut(s)	162, 1370
ZrmI	AGTACT	2 cut(s)	450, 514
Zsp2I	ATGCAT	4 cut(s)	441, 509, 576, 614

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.