Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0002831

Physical Location & Seq

Genomic Coordinates Reverse (-)

47012871 .. 47016014

Gene Structure 3144 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG254900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGAATCTAAGGAGAGTTCATTTTCGTTGGTGCCGTTGGTTCGCTTCCTCTCCTTCAACAATCTCATCGTCTGTTTCATGGGTCTCTCCTCTTCAGTTATCAAAATTCAATGCTCACAAACCAGATCCCCCTCCCGAAACCACAATTACAAGGACAGAAGCTAGGAGGAGATCCAAATATATATCTCACGATGCTGCCATCAACTTAATCAAACGTGAGAGAGATCCACAGCATGCCTTGGAAATATTTAACATGGTTTCAGAGCAAAAGGGTTTCAACCACAATAATGCCACCTATGCAACTATCCTCAGTAAGCTTGCTCAGAACAAGAAGTTCAAGGCTGTTGATGCAGTTCTCTATCAAATGAAGTATGATACCTGCAAATATCATGAGGGTATATTCCTTAATCTCATGAAGCATTTTTCAAAATCGTCTATGCATGAAAGAGTACTTGAAATGTTCTATGCTATCCAGCCAGTAGTTCGGGAAAAGCCATCTCTCAAATGCATTAGTACTTGTTTAAATCTACTGATTGAAGCGAATCAGGTTGATTTGGCCCAACAGTTTCTCATGCATTTGAAGAGTCTTAACTTGAAGCTGAATACATGCATTGTCAACATCTTGGTTAAGCACCATTGCACAAATGGGGATCTTGAATCTGCCTTCGAGGTTGTGAAGGAAATGAAAAAGTCTAAGCTTTCCTATCCTAATTTGATTACTTACTCGACTCTAATTGATGGTTTATGTCAAAGTGGAAGACTGACAGAAGCAATGGACCTGTTTGAGGAAATGATCTCAAAGGATCAGATATTGCCTGATGTGCTAACGTACAACATTTTGATTAATGGTTTTTGCCGTGGTGGGAAAGTTAATAGGGCAAGGAAGATATTGGATTTTATGAAGAGCAAAGGATGCAACCCAAATGTATTCAATTACTCAGCTCTGATGAATGGATTCTGTAAGGAGGAAAGATTTGAGGAGACCAAGGAGCTTTTGGATGAAATGAAGAGCTTTGGTATCAAACCAGATACAGTTGTCTACACTACTTTAATTGATTGCTACTGTAGGACTGGGAGAGTTGATGAAGCGATAGAGTTGCTCAAAGAGATGAAAGAAAGACGGTGCAAAGCTGATACTGTGACTTTCAATGTGATACTTGGAGGACTATGTAGAGAAGGCAGAATTGAGGATGCGCTTGAGATGCTGGACAAACTTCCTTATGAGGGTATCTATCTGAATAAGGGAAGCTACAGGATTGTGTTGAATTCCTTGTGTCAGACAGGCGAACTAAACAAAGCTAAAGAGTTGTTGAGATTGATGATGGGTAGGGGGTTTGTACCACATTATGCAACTTCAAACGAGTTGCTAGTTAGCCTCTGTGAGGCTGGATTGGCAGATGATGCAACTATAGCTTTATTTGGATTGGTGGAGTTGGGTTTCAAACCACTGCCTGATTCCTGGGCTCTTTTTGTTGAATCAATTTGCAGAGAAAGAAAGCTGCTGCCTGCATTTGAACTGCTCGATGATTTAGTTAATGAAGAGTTTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

516

Amino Acids

59.09

Weight (kDa)

8.02

Isoelectric Point (pI)

38.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	191 - 268	4.7e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	193 - 246	7.4e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	197 - 229	1e-05	PPR repeat
PPR_2	PF13041	201 - 250	3.1e-13	PPR repeat family
PPR_2	PF13041	236 - 286	2.3e-17	PPR repeat family
PPR_1	PF12854	236 - 264	3.7e-13	PPR repeat
PPR	PF01535	239 - 267	2.1e-09	PPR repeat
PPR_long	PF17177	253 - 405	1.4e-10	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	261 - 319	2.6e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	270 - 301	1.4e-12	PPR repeat
PPR_2	PF13041	272 - 321	2.2e-17	PPR repeat family
PPR	PF01535	276 - 305	6.5e-08	PPR repeat
PPR_1	PF12854	303 - 336	2.5e-10	PPR repeat
PPR_2	PF13041	307 - 351	2.9e-12	PPR repeat family
PPR	PF01535	312 - 340	2e-06	PPR repeat
PPR_3	PF13812	331 - 387	7.6e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	339 - 371	2.6e-15	PPR repeat
TPR_24	PF23276	341 - 408	7.4e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	342 - 391	2.6e-19	PPR repeat family
PPR	PF01535	345 - 375	2.9e-11	PPR repeat
PPR_1	PF12854	374 - 404	9.1e-10	PPR repeat
PPR_2	PF13041	378 - 426	4.9e-11	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002831)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G18475
fragaria_vesca	FvH4_5g35730 FvH4_5g35730 FvH4_5g35730 FvH4_5g35730 FvH4_5g35730
prunus_persica	Prupe.1G528200_v2.0.a1
pyrus_communis	pycom08g16680
rosa_chinensis	RchiOBHm_Chr4g0424581 RchiOBHm_Chr7g0237351
rosa_laevigata	RLG00000001010 RLG00000007454
rosa_multiflora	Rmu_co8091050.1_g000001 Rmu_sc0000281.1_g000013 Rmu_sc0000820.1_g000045 Rmu_sc0002603.1_g000021 Rmu_sc0002923.1_g000038 Rmu_sc0006438.1_g000010
rosa_roxburghii	Rroxscaffold_3G00224290 Rroxscaffold_3G00224300 Rroxscaffold_5G00366500
rosa_rugosa	Rorug04G0198000 Rorug07G0302800 Rorug07G0302800
rosa_samantha	Rh1CG247200 Rh1DG083900 Rh4AG254700 Rh4BG260200 Rh4CG271700 Rh4DG254900 Rh5AG212800 Rh6DG464200 Rh7AG460000 Rh7BG429900 Rh7CG477300 Rh7DG446600
rosa_wichuraiana	Rw1G023340 Rw4G022020 Rw7G038100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	386
AccB1I	GGYRCC	1 cut(s)	30
AccI	GTMKAC	1 cut(s)	1038
AclWI	GGATC	5 cut(s)	119, 165, 218, 657, 810
AcsI	RAATTY	2 cut(s)	104, 1264
AcuI	CTGAAG	1 cut(s)	77
AfaI	GTAC	4 cut(s)	450, 514, 830, 1338
AfiI	CCNNNNNNNGG	3 cut(s)	784, 1223, 1381
AjnI	CCWGG	1 cut(s)	1457
Alw26I	GTCTC	2 cut(s)	88, 974
AlwI	GGATC	5 cut(s)	119, 165, 218, 657, 810
AoxI	GGCC	1 cut(s)	555
ApeKI	GCWGC	3 cut(s)	194, 1498, 1501
ApoI	RAATTY	2 cut(s)	104, 1264
AseI	ATTAAT	1 cut(s)	843
Asp700I	GAANNNNTTC	1 cut(s)	953
AspLEI	GCGC	1 cut(s)	1195
AspS9I	GGNCC	2 cut(s)	556, 775
AvaII	GGWCC	1 cut(s)	775
BaeI	ACNNNNGTAYC	2 cut(s)	1020, 1053
BanI	GGYRCC	1 cut(s)	30
BanII	GRGCYC	1 cut(s)	1465
BbsI	GAAGAC	1 cut(s)	763
BbvI	GCAGC	3 cut(s)	181, 1485, 1488
BccI	CCATC	4 cut(s)	206, 502, 731, 1315
BceAI	ACGGC	2 cut(s)	18, 840
BcgI	CGANNNNNNTGC	2 cut(s)	1078, 1112
BciT130I	CCWGG	1 cut(s)	1459
BcoDI	GTCTC	2 cut(s)	88, 974
BfaI	CTAG	2 cut(s)	162, 1367
BfmI	CTRYAG	3 cut(s)	1063, 1249, 1407
BfuAI	ACCTGC	1 cut(s)	386
BisI	GCNGC	3 cut(s)	195, 1499, 1502
BlsI	GCNGC	3 cut(s)	196, 1500, 1503
BmcAI	AGTACT	2 cut(s)	450, 514
Bme1390I	CCNGG	1 cut(s)	1459
Bme18I	GGWCC	1 cut(s)	775
BmgT120I	GGNCC	2 cut(s)	556, 775
BmiI	GGNNCC	1 cut(s)	32
BmrFI	CCNGG	1 cut(s)	1459
BmrI	ACTGGG	1 cut(s)	1080
BmsI	GCATC	6 cut(s)	181, 337, 902, 1180, 1191, 1390
BmuI	ACTGGG	1 cut(s)	1080
BpiI	GAAGAC	1 cut(s)	763
BpuEI	CTTGAG	1 cut(s)	1217
BsaI	GGTCTC	2 cut(s)	88, 974
BsaJI	CCNNGG	4 cut(s)	237, 856, 984, 1458
Bsc4I	CCNNNNNNNGG	3 cut(s)	784, 1223, 1381
Bse1I	ACTGG	2 cut(s)	476, 1075
Bse3DI	GCAATG	2 cut(s)	634, 777
BseBI	CCWGG	1 cut(s)	1459
BseDI	CCNNGG	4 cut(s)	237, 856, 984, 1458
BseGI	GGATG	3 cut(s)	917, 1003, 1195
BseLI	CCNNNNNNNGG	3 cut(s)	784, 1223, 1381
BseMI	GCAATG	2 cut(s)	634, 777
BseMII	CTCAG	3 cut(s)	322, 335, 951
BseNI	ACTGG	2 cut(s)	476, 1075
BseRI	GAGGAG	3 cut(s)	78, 181, 992
BseXI	GCAGC	3 cut(s)	181, 1485, 1488
BshFI	GGCC	1 cut(s)	557
BshNI	GGYRCC	1 cut(s)	30
BslI	CCNNNNNNNGG	3 cut(s)	784, 1223, 1381
BsmAI	GTCTC	2 cut(s)	88, 974
BsnI	GGCC	1 cut(s)	557
Bso31I	GGTCTC	2 cut(s)	88, 974
Bsp1286I	GDGCHC	1 cut(s)	1465
Bsp143I	GATC	6 cut(s)	124, 170, 223, 649, 792, 802
BspANI	GGCC	1 cut(s)	557
BspCNI	CTCAG	3 cut(s)	321, 334, 950
BspHI	TCATGA	2 cut(s)	388, 411
BspLI	GGNNCC	1 cut(s)	32
BspMI	ACCTGC	1 cut(s)	386
BspPI	GGATC	5 cut(s)	119, 165, 218, 657, 810
BspQI	GCTCTTC	2 cut(s)	896, 1001
BspT107I	GGYRCC	1 cut(s)	30
BspTNI	GGTCTC	2 cut(s)	88, 974
BsrDI	GCAATG	2 cut(s)	634, 777
BsrI	ACTGG	2 cut(s)	476, 1075
BssECI	CCNNGG	4 cut(s)	237, 856, 984, 1458
BssMI	GATC	6 cut(s)	124, 170, 223, 649, 792, 802
BssT1I	CCWWGG	2 cut(s)	237, 984
Bst2UI	CCWGG	1 cut(s)	1459
Bst4CI	ACNGT	5 cut(s)	564, 1033, 1064, 1122, 1138
Bst6I	CTCTTC	5 cut(s)	96, 575, 896, 1001, 1533
BstAPI	GCANNNNNTGC	2 cut(s)	912, 1400
BstC8I	GCNNGC	3 cut(s)	234, 318, 1506
BstDEI	CTNAG	5 cut(s)	8, 308, 321, 693, 937
BstDSI	CCRYGG	1 cut(s)	856
BstENI	CCTNNNNNAGG	2 cut(s)	782, 1379
BstF5I	GGATG	3 cut(s)	917, 1003, 1195
BstHHI	GCGC	1 cut(s)	1195
BstKTI	GATC	6 cut(s)	127, 173, 226, 652, 795, 805
BstMAI	GTCTC	2 cut(s)	88, 974
BstMBI	GATC	6 cut(s)	124, 170, 223, 649, 792, 802
BstMWI	GCNNNNNNNGC	8 cut(s)	296, 347, 820, 912, 1201, 1391, 1400, 1409
BstNI	CCWGG	1 cut(s)	1459
BstNSI	RCATGY	2 cut(s)	236, 609
BstSCI	CCNGG	1 cut(s)	1457
BstSFI	CTRYAG	3 cut(s)	1063, 1249, 1407
BstV1I	GCAGC	3 cut(s)	181, 1485, 1488
BstV2I	GAAGAC	1 cut(s)	763
BstX2I	RGATCY	4 cut(s)	124, 170, 223, 649
BstYI	RGATCY	4 cut(s)	124, 170, 223, 649
BsuRI	GGCC	1 cut(s)	557
BtgI	CCRYGG	1 cut(s)	856
BtsCI	GGATG	3 cut(s)	917, 1003, 1195
BtsI	GCAGTG	1 cut(s)	1445
BtsIMutI	CAGTG	1 cut(s)	1445
BveI	ACCTGC	1 cut(s)	386
Cac8I	GCNNGC	3 cut(s)	234, 318, 1506
CciI	TCATGA	2 cut(s)	388, 411
CfoI	GCGC	1 cut(s)	1195
Cfr13I	GGNCC	2 cut(s)	556, 775
Csp6I	GTAC	4 cut(s)	449, 513, 829, 1337
CviAII	CATG	8 cut(s)	78, 233, 253, 389, 412, 440, 571, 606
CviQI	GTAC	4 cut(s)	449, 513, 829, 1337
DdeI	CTNAG	5 cut(s)	8, 308, 321, 693, 937
DpnI	GATC	6 cut(s)	126, 172, 225, 651, 794, 804
DpnII	GATC	6 cut(s)	124, 170, 223, 649, 792, 802
DraI	TTTAAA	1 cut(s)	522
Eam1104I	CTCTTC	5 cut(s)	96, 575, 896, 1001, 1533
EarI	CTCTTC	5 cut(s)	96, 575, 896, 1001, 1533
Eco130I	CCWWGG	2 cut(s)	237, 984
Eco24I	GRGCYC	1 cut(s)	1465
Eco31I	GGTCTC	2 cut(s)	88, 974
Eco47I	GGWCC	1 cut(s)	775
Eco57I	CTGAAG	1 cut(s)	77
EcoNI	CCTNNNNNAGG	2 cut(s)	782, 1379
EcoRI	GAATTC	1 cut(s)	1264
EcoRII	CCWGG	1 cut(s)	1457
EcoT14I	CCWWGG	2 cut(s)	237, 984
EcoT22I	ATGCAT	4 cut(s)	441, 509, 576, 611
EcoT38I	GRGCYC	1 cut(s)	1465
ErhI	CCWWGG	2 cut(s)	237, 984
FaeI	CATG	8 cut(s)	81, 236, 256, 392, 415, 443, 574, 609
FatI	CATG	8 cut(s)	77, 232, 252, 388, 411, 439, 570, 605
FblI	GTMKAC	1 cut(s)	1038
Fnu4HI	GCNGC	3 cut(s)	195, 1499, 1502
FokI	GGATG	3 cut(s)	924, 1010, 1202
FriOI	GRGCYC	1 cut(s)	1465
Fsp4HI	GCNGC	3 cut(s)	195, 1499, 1502
FspBI	CTAG	2 cut(s)	162, 1367
GlaI	GCGC	1 cut(s)	1194
GluI	GCNGC	3 cut(s)	195, 1499, 1502
HaeIII	GGCC	1 cut(s)	557
HhaI	GCGC	1 cut(s)	1195
Hin1II	CATG	8 cut(s)	81, 236, 256, 392, 415, 443, 574, 609
Hin6I	GCGC	1 cut(s)	1193
HinP1I	GCGC	1 cut(s)	1193
HincII	GTYRAC	1 cut(s)	616
HindII	GTYRAC	1 cut(s)	616
HindIII	AAGCTT	2 cut(s)	314, 695
HinfI	GANTC	8 cut(s)	4, 541, 583, 656, 727, 954, 1454, 1475
Hpy166II	GTNNAC	2 cut(s)	616, 1039
Hpy188I	TCNGA	6 cut(s)	262, 324, 807, 945, 1236, 1278
Hpy188III	TCNNGA	6 cut(s)	134, 188, 389, 412, 485, 653
Hpy8I	GTNNAC	2 cut(s)	616, 1039
HpyAV	CCTTC	4 cut(s)	63, 670, 673, 1169
HpyCH4III	ACNGT	5 cut(s)	564, 1033, 1064, 1122, 1138
HpyCH4IV	ACGT	2 cut(s)	214, 827
HpyF10VI	GCNNNNNNNGC	8 cut(s)	296, 347, 820, 912, 1201, 1391, 1400, 1409
HpyF3I	CTNAG	5 cut(s)	8, 308, 321, 693, 937
HpySE526I	ACGT	2 cut(s)	214, 827
Hsp92II	CATG	8 cut(s)	81, 236, 256, 392, 415, 443, 574, 609
HspAI	GCGC	1 cut(s)	1193
Kzo9I	GATC	6 cut(s)	124, 170, 223, 649, 792, 802
LguI	GCTCTTC	2 cut(s)	896, 1001
LmnI	GCTCC	1 cut(s)	988
Lsp1109I	GCAGC	3 cut(s)	181, 1485, 1488
LweI	GCATC	6 cut(s)	181, 337, 902, 1180, 1191, 1390
MaeI	CTAG	2 cut(s)	162, 1367
MaeII	ACGT	2 cut(s)	214, 827
MaeIII	GTNAC	1 cut(s)	1138
MalI	GATC	6 cut(s)	126, 172, 225, 651, 794, 804
MboI	GATC	6 cut(s)	124, 170, 223, 649, 792, 802
MboII	GAAGA	7 cut(s)	83, 592, 768, 895, 913, 1018, 1550
MflI	RGATCY	4 cut(s)	124, 170, 223, 649
MhlI	GDGCHC	1 cut(s)	1465
MluCI	AATT	9 cut(s)	104, 144, 709, 732, 931, 1050, 1182, 1264, 1479
MlyI	GAGTC	2 cut(s)	592, 721
Mph1103I	ATGCAT	4 cut(s)	441, 509, 576, 611
MroXI	GAANNNNTTC	1 cut(s)	953
MslI	CAYNNNNRTG	1 cut(s)	285
MspR9I	CCNGG	1 cut(s)	1459
MvaI	CCWGG	1 cut(s)	1459
MwoI	GCNNNNNNNGC	8 cut(s)	296, 347, 820, 912, 1201, 1391, 1400, 1409
NdeII	GATC	6 cut(s)	124, 170, 223, 649, 792, 802
NlaIII	CATG	8 cut(s)	81, 236, 256, 392, 415, 443, 574, 609
NlaIV	GGNNCC	1 cut(s)	32
NmuCI	GTSAC	1 cut(s)	1138
NsiI	ATGCAT	4 cut(s)	441, 509, 576, 611
NspI	RCATGY	2 cut(s)	236, 609
PaeI	GCATGC	1 cut(s)	236
PagI	TCATGA	2 cut(s)	388, 411
PciSI	GCTCTTC	2 cut(s)	896, 1001
PcsI	WCGNNNNNNNCGW	1 cut(s)	31
PdmI	GAANNNNTTC	1 cut(s)	953
PfeI	GAWTC	6 cut(s)	4, 541, 656, 954, 1454, 1475
PkrI	GCNGC	3 cut(s)	196, 1500, 1503
PleI	GAGTC	2 cut(s)	591, 721
PpsI	GAGTC	2 cut(s)	591, 721
PshBI	ATTAAT	1 cut(s)	843
Psp6I	CCWGG	1 cut(s)	1457
PspGI	CCWGG	1 cut(s)	1457
PspN4I	GGNNCC	1 cut(s)	32
PspPI	GGNCC	2 cut(s)	556, 775
PsuI	RGATCY	4 cut(s)	124, 170, 223, 649
RsaI	GTAC	4 cut(s)	450, 514, 830, 1338
RsaNI	GTAC	4 cut(s)	449, 513, 829, 1337
RseI	CAYNNNNRTG	1 cut(s)	285
SapI	GCTCTTC	2 cut(s)	896, 1001
SatI	GCNGC	3 cut(s)	195, 1499, 1502
Sau3AI	GATC	6 cut(s)	124, 170, 223, 649, 792, 802
Sau96I	GGNCC	2 cut(s)	556, 775
ScaI	AGTACT	2 cut(s)	450, 514
SchI	GAGTC	2 cut(s)	592, 721
ScrFI	CCNGG	1 cut(s)	1459
SduI	GDGCHC	1 cut(s)	1465
SfaNI	GCATC	6 cut(s)	181, 337, 902, 1180, 1191, 1390
SfcI	CTRYAG	3 cut(s)	1063, 1249, 1407
SinI	GGWCC	1 cut(s)	775
SmiMI	CAYNNNNRTG	1 cut(s)	285
SmlI	CTYRAG	1 cut(s)	1196
SmoI	CTYRAG	1 cut(s)	1196
SphI	GCATGC	1 cut(s)	236
Sse9I	AATT	9 cut(s)	104, 144, 709, 732, 931, 1050, 1182, 1264, 1479
SspI	AATATT	1 cut(s)	246
SspMI	CTAG	2 cut(s)	162, 1367
StyD4I	CCNGG	1 cut(s)	1457
StyI	CCWWGG	2 cut(s)	237, 984
TaaI	ACNGT	5 cut(s)	564, 1033, 1064, 1122, 1138
TaiI	ACGT	2 cut(s)	217, 830
TaqI	TCGA	3 cut(s)	666, 725, 1521
TasI	AATT	9 cut(s)	104, 144, 709, 732, 931, 1050, 1182, 1264, 1479
TatI	WGTACW	2 cut(s)	448, 512
TfiI	GAWTC	6 cut(s)	4, 541, 656, 954, 1454, 1475
TscAI	CASTG	1 cut(s)	1452
TseFI	GTSAC	1 cut(s)	1138
TseI	GCWGC	3 cut(s)	194, 1498, 1501
Tsp45I	GTSAC	1 cut(s)	1138
TspRI	CASTG	1 cut(s)	1452
VpaK11BI	GGWCC	1 cut(s)	775
VspI	ATTAAT	1 cut(s)	843
XagI	CCTNNNNNAGG	2 cut(s)	782, 1379
XapI	RAATTY	2 cut(s)	104, 1264
XceI	RCATGY	2 cut(s)	236, 609
XcmI	CCANNNNNNNNNTGG	2 cut(s)	641, 991
XmiI	GTMKAC	1 cut(s)	1038
XmnI	GAANNNNTTC	1 cut(s)	953
XspI	CTAG	2 cut(s)	162, 1367
ZrmI	AGTACT	2 cut(s)	450, 514
Zsp2I	ATGCAT	4 cut(s)	441, 509, 576, 611

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.