RLG00000007454

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002831
Physical Location & Seq
Forward (+)
16994563 .. 16995526
964 bp
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UTR
Exon/CDS
Intron
RLM00000007454

Sequence Viewer

Length: 912 bp
ATGGTTTCGGAGCAAAAGGGTTTCAACCACAATAATGCCACCTATGCAACTATCCTCAATAAGCTTGCTCAGTCCAAGAAGTTCAAGGCTGTTGATGCAGTTCTCTATCAAATGAAGTACGATACCTGCAAATATCATGAGGGTATATTCCTTAATCTCATGAAGCATTTTTCAAAATCGTCTATGCATGAAAGAGTACTTGAAATGTTCTATGCTATCCGGCCAGTAGTTCGGGAAAAGCCATCTCTCAAATGCATTAGTACTTGTTTAAATCTACTGATTGAAGCGAATCAGGTTGATTTGGCCCAACAGTTTCTCATGCATTTGAAGAAGAGTTGTAACTTGAAGCTGAATACATGCATTGTCAACATCTTGGTTAAGCACCATTGCACAAATGGGGATCTTGAATCTGCCTTTGAGGTTGTGAGGGAAATGAAAAAGTCTAAGCTTTCCTATCCTAATTTGATTACTTACTCGACTCTAATTGATGGTTTATGTCAAAGTGGAAGACTGACAGAAGCAATGGACCTGTTTGAGGAAATGATCTCAAAGGATCAGATATTGCCTGATGTGCTGACGTGCAACATTTTGATTAATGGTTTTTGCCGTGATGGGAAAGTTAATAGGGCAAGGAAGATATTGGATTTTATGAAGAGCAAAGGATGCAACCCAAATGTATTCAATTACTCAGCTCTGATGAATGGATTCTGTATGGAGGAGGCCAAGGAGCTTTGGACTGGGAGAGTTGATGAAGCGATAGATTTGCTCAAAGAGATGAAAGAAAGACGGTGCAAAGCTGATACTGAGACTTTCAATGTGATACTTGGAGCCTTGGAGGACTATGTAGAGAAGGCAGAATTGAGGATGCGCTTGAGATGCTGGACAAACTTCCTTATGAGGGTATCTATCTGA

Protein Analysis

304

Amino Acids

34.99

Weight (kDa)

8.69

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 114 - 146 3e-06 PPR repeat
PPR_3 PF13812 114 - 163 2.2e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 118 - 167 2.2e-13 PPR repeat family
PPR_long PF17177 118 - 242 7e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 153 - 190 7.4e-09 PPR repeat family
PPR_1 PF12854 153 - 181 1.8e-13 PPR repeat
PPR PF01535 156 - 184 1.1e-09 PPR repeat
PPR_3 PF13812 178 - 235 2.5e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 187 - 218 2.3e-10 PPR repeat
PPR_2 PF13041 189 - 237 6.9e-17 PPR repeat family
PPR PF01535 193 - 222 1.9e-07 PPR repeat
TPR_24 PF23276 196 - 279 1.8e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 220 - 260 5.6e-07 PPR repeat
PPR_2 PF13041 246 - 278 2.6e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 134
AclWI GGATC 2 cut(s) 408, 561
AcoI YGGCCR 1 cut(s) 221
AfaI GTAC 3 cut(s) 119, 198, 262
AfiI CCNNNNNNNGG 2 cut(s) 535, 898
AjiI CACGTC 1 cut(s) 579
AluBI AGCT 6 cut(s) 64, 349, 448, 692, 730, 797
AluI AGCT 6 cut(s) 64, 349, 448, 692, 730, 797
Alw26I GTCTC 1 cut(s) 800
AlwI GGATC 2 cut(s) 408, 561
AoxI GGCC 3 cut(s) 221, 303, 720
AseI ATTAAT 1 cut(s) 594
Asp700I GAANNNNTTC 1 cut(s) 704
AspLEI GCGC 1 cut(s) 870
AspS9I GGNCC 2 cut(s) 304, 526
AvaII GGWCC 1 cut(s) 526
BbsI GAAGAC 1 cut(s) 514
BccI CCATC 3 cut(s) 250, 482, 605
BceAI ACGGC 1 cut(s) 591
BcgI CGANNNNNNTGC 2 cut(s) 745, 779
BcoDI GTCTC 1 cut(s) 800
BfuAI ACCTGC 1 cut(s) 134
BmcAI AGTACT 2 cut(s) 198, 262
Bme18I GGWCC 1 cut(s) 526
BmgBI CACGTC 1 cut(s) 579
BmgT120I GGNCC 2 cut(s) 304, 526
BmiI GGNNCC 1 cut(s) 829
BmrI ACTGGG 1 cut(s) 747
BmsI GCATC 4 cut(s) 85, 653, 855, 866
BmuI ACTGGG 1 cut(s) 747
BpiI GAAGAC 1 cut(s) 514
BpuEI CTTGAG 1 cut(s) 892
BsaJI CCNNGG 2 cut(s) 723, 831
Bsc4I CCNNNNNNNGG 2 cut(s) 535, 898
Bse1I ACTGG 2 cut(s) 224, 742
Bse3DI GCAATG 2 cut(s) 385, 528
BseDI CCNNGG 2 cut(s) 723, 831
BseGI GGATG 2 cut(s) 668, 870
BseLI CCNNNNNNNGG 2 cut(s) 535, 898
BseMI GCAATG 2 cut(s) 385, 528
BseMII CTCAG 3 cut(s) 83, 702, 795
BseNI ACTGG 2 cut(s) 224, 742
BseRI GAGGAG 1 cut(s) 731
BshFI GGCC 3 cut(s) 223, 305, 722
BsiSI CCGG 1 cut(s) 220
BslI CCNNNNNNNGG 2 cut(s) 535, 898
BsmAI GTCTC 1 cut(s) 800
BsnI GGCC 3 cut(s) 223, 305, 722
Bsp143I GATC 3 cut(s) 400, 543, 553
BspANI GGCC 3 cut(s) 223, 305, 722
BspCNI CTCAG 3 cut(s) 82, 701, 796
BspHI TCATGA 2 cut(s) 136, 159
BspLI GGNNCC 1 cut(s) 829
BspMI ACCTGC 1 cut(s) 134
BspPI GGATC 2 cut(s) 408, 561
BspQI GCTCTTC 1 cut(s) 647
BsrDI GCAATG 2 cut(s) 385, 528
BsrI ACTGG 2 cut(s) 224, 742
BssECI CCNNGG 2 cut(s) 723, 831
BssMI GATC 3 cut(s) 400, 543, 553
BssT1I CCWWGG 2 cut(s) 723, 831
Bst4CI ACNGT 2 cut(s) 312, 789
Bst6I CTCTTC 2 cut(s) 326, 647
BstAPI GCANNNNNTGC 1 cut(s) 663
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 4 cut(s) 69, 444, 688, 804
BstENI CCTNNNNNAGG 1 cut(s) 533
BstF5I GGATG 2 cut(s) 668, 870
BstHHI GCGC 1 cut(s) 870
BstKTI GATC 3 cut(s) 403, 546, 556
BstMAI GTCTC 1 cut(s) 800
BstMBI GATC 3 cut(s) 400, 543, 553
BstMWI GCNNNNNNNGC 5 cut(s) 44, 95, 571, 663, 876
BstNSI RCATGY 1 cut(s) 360
BstV2I GAAGAC 1 cut(s) 514
BstX2I RGATCY 1 cut(s) 400
BstYI RGATCY 1 cut(s) 400
BsuRI GGCC 3 cut(s) 223, 305, 722
BtrI CACGTC 1 cut(s) 579
BtsCI GGATG 2 cut(s) 668, 870
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 1 cut(s) 66
CciI TCATGA 2 cut(s) 136, 159
CfoI GCGC 1 cut(s) 870
Cfr13I GGNCC 2 cut(s) 304, 526
Csp6I GTAC 3 cut(s) 118, 197, 261
CviAII CATG 5 cut(s) 137, 160, 188, 319, 357
CviQI GTAC 3 cut(s) 118, 197, 261
DdeI CTNAG 4 cut(s) 69, 444, 688, 804
DpnI GATC 3 cut(s) 402, 545, 555
DpnII GATC 3 cut(s) 400, 543, 553
DraI TTTAAA 1 cut(s) 270
EaeI YGGCCR 1 cut(s) 221
Eam1104I CTCTTC 2 cut(s) 326, 647
EarI CTCTTC 2 cut(s) 326, 647
Eco130I CCWWGG 2 cut(s) 723, 831
Eco47I GGWCC 1 cut(s) 526
EcoNI CCTNNNNNAGG 1 cut(s) 533
EcoT14I CCWWGG 2 cut(s) 723, 831
EcoT22I ATGCAT 4 cut(s) 189, 257, 324, 362
ErhI CCWWGG 2 cut(s) 723, 831
FaeI CATG 5 cut(s) 140, 163, 191, 322, 360
FatI CATG 5 cut(s) 136, 159, 187, 318, 356
FokI GGATG 2 cut(s) 675, 877
GlaI GCGC 1 cut(s) 869
HaeIII GGCC 3 cut(s) 223, 305, 722
HapII CCGG 1 cut(s) 220
HhaI GCGC 1 cut(s) 870
Hin1II CATG 5 cut(s) 140, 163, 191, 322, 360
Hin6I GCGC 1 cut(s) 868
HinP1I GCGC 1 cut(s) 868
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HindIII AAGCTT 2 cut(s) 62, 446
HinfI GANTC 4 cut(s) 289, 407, 478, 705
HpaII CCGG 1 cut(s) 220
Hpy166II GTNNAC 1 cut(s) 367
Hpy188I TCNGA 4 cut(s) 10, 558, 696, 911
Hpy188III TCNNGA 4 cut(s) 137, 160, 233, 404
Hpy8I GTNNAC 1 cut(s) 367
HpyAV CCTTC 1 cut(s) 844
HpyCH4III ACNGT 2 cut(s) 312, 789
HpyCH4IV ACGT 1 cut(s) 578
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 95, 571, 663, 876
HpyF3I CTNAG 4 cut(s) 69, 444, 688, 804
HpySE526I ACGT 1 cut(s) 578
Hsp92II CATG 5 cut(s) 140, 163, 191, 322, 360
HspAI GCGC 1 cut(s) 868
Kzo9I GATC 3 cut(s) 400, 543, 553
LguI GCTCTTC 1 cut(s) 647
LmnI GCTCC 3 cut(s) 10, 727, 827
LpnPI CCDG 8 cut(s) 139, 233, 237, 278, 542, 579, 723, 865
LweI GCATC 4 cut(s) 85, 653, 855, 866
MaeII ACGT 1 cut(s) 578
MaeIII GTNAC 1 cut(s) 338
MalI GATC 3 cut(s) 402, 545, 555
MboI GATC 3 cut(s) 400, 543, 553
MboII GAAGA 5 cut(s) 340, 343, 519, 646, 664
MflI RGATCY 1 cut(s) 400
MluCI AATT 4 cut(s) 460, 483, 682, 857
MlyI GAGTC 1 cut(s) 472
Mph1103I ATGCAT 4 cut(s) 189, 257, 324, 362
MroXI GAANNNNTTC 1 cut(s) 704
MseI TTAA 5 cut(s) 153, 269, 378, 594, 621
MslI CAYNNNNRTG 1 cut(s) 33
MspI CCGG 1 cut(s) 220
MwoI GCNNNNNNNGC 5 cut(s) 44, 95, 571, 663, 876
NdeII GATC 3 cut(s) 400, 543, 553
NlaIII CATG 5 cut(s) 140, 163, 191, 322, 360
NlaIV GGNNCC 1 cut(s) 829
NsiI ATGCAT 4 cut(s) 189, 257, 324, 362
NspI RCATGY 1 cut(s) 360
PagI TCATGA 2 cut(s) 136, 159
PciSI GCTCTTC 1 cut(s) 647
PdmI GAANNNNTTC 1 cut(s) 704
PfeI GAWTC 3 cut(s) 289, 407, 705
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
PshBI ATTAAT 1 cut(s) 594
PspN4I GGNNCC 1 cut(s) 829
PspPI GGNCC 2 cut(s) 304, 526
PsuI RGATCY 1 cut(s) 400
RsaI GTAC 3 cut(s) 119, 198, 262
RsaNI GTAC 3 cut(s) 118, 197, 261
RseI CAYNNNNRTG 1 cut(s) 33
SapI GCTCTTC 1 cut(s) 647
SaqAI TTAA 5 cut(s) 153, 269, 378, 594, 621
Sau3AI GATC 3 cut(s) 400, 543, 553
Sau96I GGNCC 2 cut(s) 304, 526
ScaI AGTACT 2 cut(s) 198, 262
SchI GAGTC 1 cut(s) 472
SfaNI GCATC 4 cut(s) 85, 653, 855, 866
SinI GGWCC 1 cut(s) 526
SmiMI CAYNNNNRTG 1 cut(s) 33
SmlI CTYRAG 1 cut(s) 871
SmoI CTYRAG 1 cut(s) 871
Sse9I AATT 4 cut(s) 460, 483, 682, 857
StyI CCWWGG 2 cut(s) 723, 831
TaaI ACNGT 2 cut(s) 312, 789
TaiI ACGT 1 cut(s) 581
TaqI TCGA 1 cut(s) 476
TasI AATT 4 cut(s) 460, 483, 682, 857
TatI WGTACW 2 cut(s) 196, 260
TfiI GAWTC 3 cut(s) 289, 407, 705
Tru1I TTAA 5 cut(s) 153, 269, 378, 594, 621
Tru9I TTAA 5 cut(s) 153, 269, 378, 594, 621
TspDTI ATGAA 8 cut(s) 128, 176, 204, 449, 665, 713, 765, 791
VpaK11BI GGWCC 1 cut(s) 526
VspI ATTAAT 1 cut(s) 594
XagI CCTNNNNNAGG 1 cut(s) 533
XceI RCATGY 1 cut(s) 360
XcmI CCANNNNNNNNNTGG 1 cut(s) 392
XmnI GAANNNNTTC 1 cut(s) 704
ZrmI AGTACT 2 cut(s) 198, 262
Zsp2I ATGCAT 4 cut(s) 189, 257, 324, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.