Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0002831

Physical Location & Seq

Genomic Coordinates Forward (+)

16750028 .. 16751581

Gene Structure 1554 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g16680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1554 bp

ATGAATGCTATGAGAGTGAATAAAAGTCAGTGCCGTTGGTTCTCATCCTCTCCTTCGACACTCTCATCGTCACTTCCATGGATCTCTCCTCTTCACTTTTCAAGAGTCAAACTTCACAAACCAGACCCCCCACCGGAAACCACAGACACCCAGACAGAAACGAGGAGGAAAGGCAAATATATTTCTCACGAATCTGCCATCAACTTAATCAAACGTGGGAGAGATCCACAGCATGCCTTGGAAATATTTAACATGGTATCTGAACAAAAGGGTTTTAATCACAACAATGCCACCTATGGAACCATCCTCCAGAAGCTTGCTCAGTCCAAGAAGTTCAAGGCTATCGATGCAATTCTTCATCAAATGACCTATGAGACCTGCAAATTTCATGAGGGTATATTCCTTAATCTCATGAGCCATTTTTCAAAATCCTCTATGCATGAAAGAGTGCTTGAAATGTTCTATGCTATCCAGCCAATAGTTCGAGAAAAACCGTCTCTCAAATCCATCAGTACTTGTCTCAATTTACTGATTGAAGCAAATCAGGTTGATTTGGCCCGACAATTTCTCACGCATTTGAAAAAGAACTTTAGCTTCAAGCCAAATACATGCATTGTCAACATCTTGGTTAAGCACCATTGCAAGAATGGGGATCTTGATTCTGCTTTTGAGGTTCTGAAGGAAATGAAAAGGTTCAAAATTTCTTACCCTAATTTGATTACATATTCAACTCTCTTGGGTGGTCTCTGTGAAAGTGGAAGACTCGCAGAAGCAATGGAGTTGTTCGAAGAAATGATCTCAAAGGACCAAATCTTGCCTGATGCCCTAACTTACAACATTTTGATTAATGGGTTTTGCCATGGTGGCAAAGTCGACAGGGCTAGGAAGATATTGGAGTTTATGAAGAGCAATGGATGCAACCCAAATGTATTCAATTATACCGCTCTGATGAACGGTTTATGTAAAGAGAAGAGATTGAAGGAGGCCAGGGAAGTTTTCCATGAAATGATGAGCTTCGGTATAAAGCCTGATACGGTTGGATACACTGGTCTGATTCATTGCTGCTGTAGGACTGGGAAAGTGAATGAAGCTATAGAGTTGCTCAAAGAGATGAAAGAGAGAGAGTGCAAAGCTGATACCGTGACTTTCAATGTGATACTCGGAGGGTTGTGCAGAGAAGGCAGAATTGAGGATGCTCTTGAGATGCTGGAGAAACTTCCTTACGAGGGTGTCTATCTCAACAAGGCAAGCTACAGGATTGTGTTGAATCTCTTGTGTCAAAAAGGTGAATTGAAGAAGGCGACTGCGTTGTTGGGGTTAATGATGGGTAGGGGGTTCATACCACATTATGCAACTTCAAATGAGTTGCTGGTTCGCATTTCTGAGGCTGGAATGGCAGAAGAGGCGTCCATGGCTTTGTTTGGATTAATGGAGATGGGGTTTAAGCCACTGCCTGATTCCTGGGCTGTTTTGGTTGAATCAATCTGCAGAGAAAGAAAGCTTCTGTCTGCATTTGAACTTCTCGATGAGTTGGTTGTTGGAGAGCAGGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

518

Amino Acids

58.72

Weight (kDa)

8.55

Isoelectric Point (pI)

32.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	186 - 268	1.3e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	199 - 230	9.7e-09	PPR repeat
PPR_3	PF13812	199 - 247	7e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	201 - 250	2.1e-13	PPR repeat family
PPR_2	PF13041	237 - 287	4.7e-16	PPR repeat family
PPR_1	PF12854	237 - 265	1.8e-11	PPR repeat
PPR	PF01535	240 - 268	1.8e-07	PPR repeat
PPR_3	PF13812	261 - 318	1.7e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	271 - 302	8.7e-11	PPR repeat
PPR_2	PF13041	273 - 322	2.7e-16	PPR repeat family
PPR	PF01535	277 - 306	6.8e-08	PPR repeat
PPR_1	PF12854	304 - 336	2.2e-11	PPR repeat
PPR_2	PF13041	308 - 357	1.3e-15	PPR repeat family
TPR_24	PF23276	312 - 403	2.3e-07	Fungal tetratrico peptide repeats
PPR	PF01535	313 - 341	1.4e-07	PPR repeat
PPR_long	PF17177	324 - 437	2.1e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	332 - 388	8.4e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	340 - 372	1.5e-11	PPR repeat
PPR_2	PF13041	343 - 392	3.4e-17	PPR repeat family
PPR	PF01535	348 - 376	3.7e-08	PPR repeat
PPR_1	PF12854	375 - 405	1.2e-09	PPR repeat
PPR_2	PF13041	379 - 427	5.4e-11	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002831)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G18475
fragaria_vesca	FvH4_5g35730 FvH4_5g35730 FvH4_5g35730 FvH4_5g35730 FvH4_5g35730
prunus_persica	Prupe.1G528200_v2.0.a1
pyrus_communis	pycom08g16680
rosa_chinensis	RchiOBHm_Chr4g0424581 RchiOBHm_Chr7g0237351
rosa_laevigata	RLG00000001010 RLG00000007454
rosa_multiflora	Rmu_co8091050.1_g000001 Rmu_sc0000281.1_g000013 Rmu_sc0000820.1_g000045 Rmu_sc0002603.1_g000021 Rmu_sc0002923.1_g000038 Rmu_sc0006438.1_g000010
rosa_roxburghii	Rroxscaffold_3G00224290 Rroxscaffold_3G00224300 Rroxscaffold_5G00366500
rosa_rugosa	Rorug04G0198000 Rorug07G0302800 Rorug07G0302800
rosa_samantha	Rh1CG247200 Rh1DG083900 Rh4AG254700 Rh4BG260200 Rh4CG271700 Rh4DG254900 Rh5AG212800 Rh6DG464200 Rh7AG460000 Rh7BG429900 Rh7CG477300 Rh7DG446600
rosa_wichuraiana	Rw1G023340 Rw4G022020 Rw7G038100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	386
AccBSI	CCGCTC	1 cut(s)	944
AccI	GTMKAC	1 cut(s)	873
AciI	CCGC	1 cut(s)	942
AclWI	GGATC	3 cut(s)	89, 218, 660
AcsI	RAATTY	2 cut(s)	383, 699
AcuI	CTGAAG	1 cut(s)	698
AcyI	GRCGYC	1 cut(s)	1406
AfaI	GTAC	1 cut(s)	514
AfiI	CCNNNNNNNGG	2 cut(s)	133, 1226
AjnI	CCWGG	2 cut(s)	986, 1460
AluBI	AGCT	7 cut(s)	316, 594, 1014, 1091, 1133, 1251, 1501
AluI	AGCT	7 cut(s)	316, 594, 1014, 1091, 1133, 1251, 1501
Alw26I	GTCTC	4 cut(s)	368, 501, 524, 749
AlwI	GGATC	3 cut(s)	89, 218, 660
AoxI	GGCC	2 cut(s)	555, 984
ApeKI	GCWGC	1 cut(s)	1062
ApoI	RAATTY	2 cut(s)	383, 699
AseI	ATTAAT	2 cut(s)	846, 1427
Asp700I	GAANNNNTTC	1 cut(s)	692
AspS9I	GGNCC	2 cut(s)	556, 805
AsuHPI	GGTGA	1 cut(s)	1298
AsuII	TTCGAA	1 cut(s)	786
AvaII	GGWCC	1 cut(s)	805
BaeI	ACNNNNGTAYC	2 cut(s)	1033, 1066
BbsI	GAAGAC	1 cut(s)	766
BbvI	GCAGC	1 cut(s)	1049
BccI	CCATC	5 cut(s)	206, 311, 515, 1318, 1429
BceAI	ACGGC	1 cut(s)	18
BciT130I	CCWGG	2 cut(s)	988, 1462
BciVI	GTATCC	1 cut(s)	1034
BcoDI	GTCTC	4 cut(s)	368, 501, 524, 749
BfaI	CTAG	1 cut(s)	882
BfmI	CTRYAG	4 cut(s)	1066, 1092, 1252, 1486
BfuAI	ACCTGC	1 cut(s)	386
BfuI	GTATCC	1 cut(s)	1034
BglI	GCCNNNNNGGC	1 cut(s)	864
BisI	GCNGC	1 cut(s)	1063
BlsI	GCNGC	1 cut(s)	1064
BmcAI	AGTACT	1 cut(s)	514
Bme1390I	CCNGG	2 cut(s)	988, 1462
Bme18I	GGWCC	1 cut(s)	805
BmgT120I	GGNCC	2 cut(s)	556, 805
BmiI	GGNNCC	1 cut(s)	301
BmrFI	CCNGG	2 cut(s)	988, 1462
BmrI	ACTGGG	1 cut(s)	1083
BmsI	GCATC	5 cut(s)	337, 811, 905, 1183, 1194
BmuI	ACTGGG	1 cut(s)	1083
BpiI	GAAGAC	1 cut(s)	766
BpmI	CTGGAG	2 cut(s)	293, 1229
Bpu14I	TTCGAA	1 cut(s)	786
BpuEI	CTTGAG	1 cut(s)	1220
Bsa29I	ATCGAT	1 cut(s)	345
BsaHI	GRCGYC	1 cut(s)	1406
BsaI	GGTCTC	2 cut(s)	368, 749
BsaJI	CCNNGG	6 cut(s)	77, 237, 859, 987, 1410, 1461
BsaWI	WCCGGW	1 cut(s)	133
BsaXI	ACNNNNNCTCC	2 cut(s)	1424, 1454
Bsc4I	CCNNNNNNNGG	2 cut(s)	133, 1226
Bse1I	ACTGG	2 cut(s)	1051, 1078
Bse3DI	GCAATG	4 cut(s)	637, 780, 916, 1057
BseBI	CCWGG	2 cut(s)	988, 1462
BseCI	ATCGAT	1 cut(s)	345
BseDI	CCNNGG	6 cut(s)	77, 237, 859, 987, 1410, 1461
BseGI	GGATG	4 cut(s)	44, 303, 920, 1198
BseLI	CCNNNNNNNGG	2 cut(s)	133, 1226
BseMI	GCAATG	4 cut(s)	637, 780, 916, 1057
BseMII	CTCAG	2 cut(s)	335, 1374
BseNI	ACTGG	2 cut(s)	1051, 1078
BseRI	GAGGAG	2 cut(s)	78, 178
BseXI	GCAGC	1 cut(s)	1049
BsgI	GTGCAG	1 cut(s)	1192
BshFI	GGCC	2 cut(s)	557, 986
BshVI	ATCGAT	1 cut(s)	345
BsiSI	CCGG	1 cut(s)	134
BslI	CCNNNNNNNGG	2 cut(s)	133, 1226
BsmAI	GTCTC	4 cut(s)	368, 501, 524, 749
BsmBI	CGTCTC	1 cut(s)	501
BsmI	GAATGC	1 cut(s)	10
BsnI	GGCC	2 cut(s)	557, 986
Bso31I	GGTCTC	2 cut(s)	368, 749
Bsp119I	TTCGAA	1 cut(s)	786
Bsp143I	GATC	4 cut(s)	81, 223, 652, 795
Bsp19I	CCATGG	3 cut(s)	77, 859, 1410
BspACI	CCGC	1 cut(s)	942
BspANI	GGCC	2 cut(s)	557, 986
BspCNI	CTCAG	2 cut(s)	334, 1375
BspDI	ATCGAT	1 cut(s)	345
BspHI	TCATGA	2 cut(s)	388, 411
BspLI	GGNNCC	1 cut(s)	301
BspMAI	CTGCAG	1 cut(s)	1490
BspMI	ACCTGC	1 cut(s)	386
BspPI	GGATC	3 cut(s)	89, 218, 660
BspQI	GCTCTTC	1 cut(s)	899
BspT104I	TTCGAA	1 cut(s)	786
BspTNI	GGTCTC	2 cut(s)	368, 749
BsrBI	CCGCTC	1 cut(s)	944
BsrDI	GCAATG	4 cut(s)	637, 780, 916, 1057
BsrI	ACTGG	2 cut(s)	1051, 1078
BssECI	CCNNGG	6 cut(s)	77, 237, 859, 987, 1410, 1461
BssMI	GATC	4 cut(s)	81, 223, 652, 795
BssNI	GRCGYC	1 cut(s)	1406
BssT1I	CCWWGG	4 cut(s)	77, 237, 859, 1410
Bst2UI	CCWGG	2 cut(s)	988, 1462
Bst4CI	ACNGT	4 cut(s)	495, 956, 1036, 1141
Bst6I	CTCTTC	4 cut(s)	96, 899, 965, 1395
BstACI	GRCGYC	1 cut(s)	1406
BstAPI	GCANNNNNTGC	1 cut(s)	915
BstBI	TTCGAA	1 cut(s)	786
BstC8I	GCNNGC	3 cut(s)	234, 318, 1249
BstDEI	CTNAG	2 cut(s)	321, 1383
BstDSI	CCRYGG	3 cut(s)	77, 859, 1410
BstF5I	GGATG	4 cut(s)	44, 303, 920, 1198
BstKTI	GATC	4 cut(s)	84, 226, 655, 798
BstMAI	GTCTC	4 cut(s)	368, 501, 524, 749
BstMBI	GATC	4 cut(s)	81, 223, 652, 795
BstMWI	GCNNNNNNNGC	7 cut(s)	347, 864, 915, 1179, 1394, 1403, 1412
BstNI	CCWGG	2 cut(s)	988, 1462
BstNSI	RCATGY	2 cut(s)	236, 612
BstSCI	CCNGG	2 cut(s)	986, 1460
BstSFI	CTRYAG	4 cut(s)	1066, 1092, 1252, 1486
BstV1I	GCAGC	1 cut(s)	1049
BstV2I	GAAGAC	1 cut(s)	766
BstX2I	RGATCY	3 cut(s)	81, 223, 652
BstYI	RGATCY	3 cut(s)	81, 223, 652
Bsu15I	ATCGAT	1 cut(s)	345
BsuI	GTATCC	1 cut(s)	1034
BsuRI	GGCC	2 cut(s)	557, 986
BsuTUI	ATCGAT	1 cut(s)	345
BtgI	CCRYGG	3 cut(s)	77, 859, 1410
BtsCI	GGATG	4 cut(s)	44, 303, 920, 1198
BtsI	GCAGTG	1 cut(s)	1448
BtsIMutI	CAGTG	3 cut(s)	35, 1044, 1448
BveI	ACCTGC	1 cut(s)	386
Cac8I	GCNNGC	3 cut(s)	234, 318, 1249
CciI	TCATGA	2 cut(s)	388, 411
Cfr13I	GGNCC	2 cut(s)	556, 805
ClaI	ATCGAT	1 cut(s)	345
CseI	GACGC	1 cut(s)	1395
Csp6I	GTAC	1 cut(s)	513
CviQI	GTAC	1 cut(s)	513
DdeI	CTNAG	2 cut(s)	321, 1383
DpnI	GATC	4 cut(s)	83, 225, 654, 797
DpnII	GATC	4 cut(s)	81, 223, 652, 795
Eam1104I	CTCTTC	4 cut(s)	96, 899, 965, 1395
EarI	CTCTTC	4 cut(s)	96, 899, 965, 1395
Eco130I	CCWWGG	4 cut(s)	77, 237, 859, 1410
Eco31I	GGTCTC	2 cut(s)	368, 749
Eco47I	GGWCC	1 cut(s)	805
Eco57I	CTGAAG	1 cut(s)	698
EcoRII	CCWGG	2 cut(s)	986, 1460
EcoT14I	CCWWGG	4 cut(s)	77, 237, 859, 1410
EcoT22I	ATGCAT	2 cut(s)	441, 614
ErhI	CCWWGG	4 cut(s)	77, 237, 859, 1410
Esp3I	CGTCTC	1 cut(s)	501
FblI	GTMKAC	1 cut(s)	873
Fnu4HI	GCNGC	1 cut(s)	1063
FokI	GGATG	4 cut(s)	31, 290, 927, 1205
Fsp4HI	GCNGC	1 cut(s)	1063
FspBI	CTAG	1 cut(s)	882
GluI	GCNGC	1 cut(s)	1063
GsuI	CTGGAG	2 cut(s)	293, 1229
HaeIII	GGCC	2 cut(s)	557, 986
HapII	CCGG	1 cut(s)	134
HgaI	GACGC	1 cut(s)	1395
Hin1I	GRCGYC	1 cut(s)	1406
HincII	GTYRAC	2 cut(s)	619, 874
HindII	GTYRAC	2 cut(s)	619, 874
HindIII	AAGCTT	2 cut(s)	314, 1499
HinfI	GANTC	8 cut(s)	105, 191, 659, 762, 1054, 1267, 1457, 1478
HpaII	CCGG	1 cut(s)	134
HphI	GGTGA	1 cut(s)	1298
Hpy166II	GTNNAC	2 cut(s)	619, 874
Hpy188I	TCNGA	6 cut(s)	262, 678, 948, 1053, 1163, 1384
Hpy188III	TCNNGA	9 cut(s)	102, 188, 310, 389, 412, 485, 656, 1199, 1523
Hpy8I	GTNNAC	2 cut(s)	619, 874
HpyAV	CCTTC	5 cut(s)	63, 673, 973, 1172, 1291
HpyCH4III	ACNGT	4 cut(s)	495, 956, 1036, 1141
HpyCH4IV	ACGT	1 cut(s)	214
HpyF10VI	GCNNNNNNNGC	7 cut(s)	347, 864, 915, 1179, 1394, 1403, 1412
HpyF3I	CTNAG	2 cut(s)	321, 1383
HpySE526I	ACGT	1 cut(s)	214
Hsp92I	GRCGYC	1 cut(s)	1406
Kzo9I	GATC	4 cut(s)	81, 223, 652, 795
LguI	GCTCTTC	1 cut(s)	899
Lsp1109I	GCAGC	1 cut(s)	1049
LweI	GCATC	5 cut(s)	337, 811, 905, 1183, 1194
MaeI	CTAG	1 cut(s)	882
MaeII	ACGT	1 cut(s)	214
MaeIII	GTNAC	2 cut(s)	69, 1141
MalI	GATC	4 cut(s)	83, 225, 654, 797
MbiI	CCGCTC	1 cut(s)	944
MboI	GATC	4 cut(s)	81, 223, 652, 795
MboII	GAAGA	9 cut(s)	83, 347, 771, 800, 898, 916, 982, 1306, 1412
MflI	RGATCY	3 cut(s)	81, 223, 652
MluCI	AATT	9 cut(s)	351, 383, 523, 563, 699, 712, 934, 1185, 1289
MlyI	GAGTC	2 cut(s)	114, 756
MmeI	TCCRAC	2 cut(s)	1018, 1519
Mph1103I	ATGCAT	2 cut(s)	441, 614
MroXI	GAANNNNTTC	1 cut(s)	692
MslI	CAYNNNNRTG	2 cut(s)	76, 285
MspI	CCGG	1 cut(s)	134
MspR9I	CCNGG	2 cut(s)	988, 1462
Mva1269I	GAATGC	1 cut(s)	10
MvaI	CCWGG	2 cut(s)	988, 1462
MwoI	GCNNNNNNNGC	7 cut(s)	347, 864, 915, 1179, 1394, 1403, 1412
NcoI	CCATGG	3 cut(s)	77, 859, 1410
NdeII	GATC	4 cut(s)	81, 223, 652, 795
NlaIV	GGNNCC	1 cut(s)	301
NmuCI	GTSAC	2 cut(s)	69, 1141
NsiI	ATGCAT	2 cut(s)	441, 614
NspI	RCATGY	2 cut(s)	236, 612
NspV	TTCGAA	1 cut(s)	786
PaeI	GCATGC	1 cut(s)	236
PagI	TCATGA	2 cut(s)	388, 411
PciSI	GCTCTTC	1 cut(s)	899
PctI	GAATGC	1 cut(s)	10
PdmI	GAANNNNTTC	1 cut(s)	692
PfeI	GAWTC	6 cut(s)	191, 659, 1054, 1267, 1457, 1478
PkrI	GCNGC	1 cut(s)	1064
PleI	GAGTC	2 cut(s)	113, 756
PpsI	GAGTC	2 cut(s)	113, 756
PshBI	ATTAAT	2 cut(s)	846, 1427
Psp6I	CCWGG	2 cut(s)	986, 1460
PspGI	CCWGG	2 cut(s)	986, 1460
PspN4I	GGNNCC	1 cut(s)	301
PspPI	GGNCC	2 cut(s)	556, 805
PstI	CTGCAG	1 cut(s)	1490
PsuI	RGATCY	3 cut(s)	81, 223, 652
RsaI	GTAC	1 cut(s)	514
RsaNI	GTAC	1 cut(s)	513
RseI	CAYNNNNRTG	2 cut(s)	76, 285
SalI	GTCGAC	1 cut(s)	872
SapI	GCTCTTC	1 cut(s)	899
SatI	GCNGC	1 cut(s)	1063
Sau3AI	GATC	4 cut(s)	81, 223, 652, 795
Sau96I	GGNCC	2 cut(s)	556, 805
ScaI	AGTACT	1 cut(s)	514
SchI	GAGTC	2 cut(s)	114, 756
ScrFI	CCNGG	2 cut(s)	988, 1462
SfaNI	GCATC	5 cut(s)	337, 811, 905, 1183, 1194
SfcI	CTRYAG	4 cut(s)	1066, 1092, 1252, 1486
SfuI	TTCGAA	1 cut(s)	786
SinI	GGWCC	1 cut(s)	805
SmiMI	CAYNNNNRTG	2 cut(s)	76, 285
SmlI	CTYRAG	1 cut(s)	1199
SmoI	CTYRAG	1 cut(s)	1199
SphI	GCATGC	1 cut(s)	236
Sse9I	AATT	9 cut(s)	351, 383, 523, 563, 699, 712, 934, 1185, 1289
SsiI	CCGC	1 cut(s)	942
SspI	AATATT	1 cut(s)	246
SspMI	CTAG	1 cut(s)	882
StyD4I	CCNGG	2 cut(s)	986, 1460
StyI	CCWWGG	4 cut(s)	77, 237, 859, 1410
TaaI	ACNGT	4 cut(s)	495, 956, 1036, 1141
TaiI	ACGT	1 cut(s)	217
TaqI	TCGA	6 cut(s)	56, 345, 484, 786, 873, 1524
TasI	AATT	9 cut(s)	351, 383, 523, 563, 699, 712, 934, 1185, 1289
TatI	WGTACW	1 cut(s)	512
TfiI	GAWTC	6 cut(s)	191, 659, 1054, 1267, 1457, 1478
TscAI	CASTG	3 cut(s)	35, 1051, 1455
TseFI	GTSAC	2 cut(s)	69, 1141
TseI	GCWGC	1 cut(s)	1062
Tsp45I	GTSAC	2 cut(s)	69, 1141
TspRI	CASTG	3 cut(s)	35, 1051, 1455
VpaK11BI	GGWCC	1 cut(s)	805
VspI	ATTAAT	2 cut(s)	846, 1427
XapI	RAATTY	2 cut(s)	383, 699
XceI	RCATGY	2 cut(s)	236, 612
XcmI	CCANNNNNNNNNTGG	1 cut(s)	644
XmiI	GTMKAC	1 cut(s)	873
XmnI	GAANNNNTTC	1 cut(s)	692
XspI	CTAG	1 cut(s)	882
ZrmI	AGTACT	1 cut(s)	514
Zsp2I	ATGCAT	2 cut(s)	441, 614

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.