FvH4_5g35950

Dihydrofolate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008449
Physical Location & Seq
Reverse (-)
26354745 .. 26356442
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35950.t1

Sequence Viewer

Length: 681 bp
ATGGGAAGCGAGGGTTGTGCCGCTTCGAGGAAACCCAGGTTCCTCTGCCTTCATGGGTTCCGAACCAGCGGCCTGATCCTCAAGACCCAACTCGGTAACAAGTGGCCGGAGTCGGTGCTCCGGCACTTCGACCTCGACTTTCTCGATGCGCCTTTTCCGGCCATTGGAAAATCCGAGGTCGAAGGCATTTTCGATCCTCCTTATTACGAGTGGTTCCAGTTCAACAAGGAATTTTCGGAGTACACAAATTTTGACGAGTGTCTTGAATTCATTGAAGATTACATGGTCAAGAATGGACCTTTTGATGGGCTACTTGGTTTTTCCCAGGGGGCAATACTGTCAGCTGCTCTGCCTGGTCTACAAGCAAAGGGTTTGGCATTGACCAAAGTACCTAAAATTAAGTTCTTGATAATAATTGGAGGGGCAAAATTCAGGTCGCCTACCGTGGTTGAAAATGCTTATTCCACTCCGATTCAAATTCCGTCTCTTCACTTTTTAGGAGAGATGGACTTCTTGAAGCAATATGGACTTGAACTGCTGGAAAACTGCGTGGATCCTTCAGTGATTCATCATCCCAAGGGCCACACGATACCAAGACTCGATGAAAAGGGTTTAGAGACCATTATGAGCTTCATTGAGACGATTCAGAAGAAGTTGGACGAGAAGGAGGAGAAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1

Protein Analysis

227

Amino Acids

25.49

Weight (kDa)

5.43

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 9 - 202 4e-51 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 358
AciI CCGC 2 cut(s) 21, 69
AclWI GGATC 4 cut(s) 70, 188, 548, 561
AcoI YGGCCR 2 cut(s) 104, 159
AcsI RAATTY 5 cut(s) 230, 247, 266, 428, 477
AcuI CTGAAG 1 cut(s) 543
AfaI GTAC 2 cut(s) 242, 390
AfiI CCNNNNNNNGG 3 cut(s) 27, 164, 305
AgsI TTSAA 7 cut(s) 223, 266, 275, 452, 476, 517, 533
AjnI CCWGG 3 cut(s) 35, 324, 352
AluBI AGCT 2 cut(s) 344, 630
AluI AGCT 2 cut(s) 344, 630
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 3 cut(s) 489, 611, 632
AlwI GGATC 4 cut(s) 70, 188, 548, 561
AoxI GGCC 4 cut(s) 70, 104, 159, 580
ApeKI GCWGC 1 cut(s) 344
ApoI RAATTY 5 cut(s) 230, 247, 266, 428, 477
ArsI GACNNNNNNTTYG 2 cut(s) 419, 451
AspLEI GCGC 1 cut(s) 151
AspS9I GGNCC 2 cut(s) 296, 580
AvaII GGWCC 1 cut(s) 296
BaeI ACNNNNGTAYC 2 cut(s) 372, 405
BamHI GGATCC 1 cut(s) 553
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 1 cut(s) 331
BccI CCATC 2 cut(s) 299, 499
BcgI CGANNNNNNTGC 1 cut(s) 33
BciT130I CCWGG 3 cut(s) 37, 326, 354
BcoDI GTCTC 3 cut(s) 489, 611, 632
BisI GCNGC 3 cut(s) 21, 70, 345
BlsI GCNGC 3 cut(s) 22, 71, 346
Bme1390I CCNGG 3 cut(s) 37, 326, 354
Bme18I GGWCC 1 cut(s) 296
BmgT120I GGNCC 2 cut(s) 296, 580
BmiI GGNNCC 4 cut(s) 41, 59, 215, 555
BmrFI CCNGG 3 cut(s) 37, 326, 354
BmsI GCATC 1 cut(s) 136
BoxI GACNNNNGTC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 611
BsaJI CCNNGG 6 cut(s) 35, 174, 324, 325, 444, 576
BsaXI ACNNNNNCTCC 1 cut(s) 662
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 164, 305
Bse1I ACTGG 1 cut(s) 217
BseBI CCWGG 3 cut(s) 37, 326, 354
BseDI CCNNGG 6 cut(s) 35, 174, 324, 325, 444, 576
BseGI GGATG 1 cut(s) 571
BseLI CCNNNNNNNGG 3 cut(s) 27, 164, 305
BseNI ACTGG 1 cut(s) 217
BseXI GCAGC 1 cut(s) 331
BshFI GGCC 4 cut(s) 72, 106, 161, 582
BsiHKAI GWGCWC 1 cut(s) 120
BsiSI CCGG 3 cut(s) 107, 121, 158
BslI CCNNNNNNNGG 3 cut(s) 27, 164, 305
BsmAI GTCTC 3 cut(s) 489, 611, 632
BsmBI CGTCTC 2 cut(s) 489, 632
BsnI GGCC 4 cut(s) 72, 106, 161, 582
Bso31I GGTCTC 1 cut(s) 611
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 3 cut(s) 75, 193, 553
BspACI CCGC 2 cut(s) 21, 69
BspANI GGCC 4 cut(s) 72, 106, 161, 582
BspLI GGNNCC 4 cut(s) 41, 59, 215, 555
BspPI GGATC 4 cut(s) 70, 188, 548, 561
BspTNI GGTCTC 1 cut(s) 611
BsrI ACTGG 1 cut(s) 217
BssECI CCNNGG 6 cut(s) 35, 174, 324, 325, 444, 576
BssMI GATC 3 cut(s) 75, 193, 553
BssT1I CCWWGG 1 cut(s) 576
Bst2UI CCWGG 3 cut(s) 37, 326, 354
Bst4CI ACNGT 3 cut(s) 339, 445, 678
Bst6I CTCTTC 1 cut(s) 492
BstDSI CCRYGG 1 cut(s) 444
BstF5I GGATG 1 cut(s) 571
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 3 cut(s) 78, 196, 556
BstMAI GTCTC 3 cut(s) 489, 611, 632
BstMBI GATC 3 cut(s) 75, 193, 553
BstNI CCWGG 3 cut(s) 37, 326, 354
BstPAI GACNNNNGTC 1 cut(s) 258
BstSCI CCNGG 3 cut(s) 35, 324, 352
BstV1I GCAGC 1 cut(s) 331
BstX2I RGATCY 1 cut(s) 553
BstYI RGATCY 1 cut(s) 553
BsuRI GGCC 4 cut(s) 72, 106, 161, 582
BtgI CCRYGG 1 cut(s) 444
BtsCI GGATG 1 cut(s) 571
BtsIMutI CAGTG 1 cut(s) 567
CfoI GCGC 1 cut(s) 151
Cfr13I GGNCC 2 cut(s) 296, 580
Csp6I GTAC 2 cut(s) 241, 389
CviAII CATG 2 cut(s) 53, 283
CviJI RGCY 7 cut(s) 72, 106, 161, 310, 344, 582, 630
CviKI_1 RGCY 7 cut(s) 72, 106, 161, 310, 344, 582, 630
CviQI GTAC 2 cut(s) 241, 389
DpnI GATC 3 cut(s) 77, 195, 555
DpnII GATC 3 cut(s) 75, 193, 553
EaeI YGGCCR 2 cut(s) 104, 159
Eam1104I CTCTTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 492
Eco130I CCWWGG 1 cut(s) 576
Eco31I GGTCTC 1 cut(s) 611
Eco47I GGWCC 1 cut(s) 296
Eco57I CTGAAG 1 cut(s) 543
EcoRI GAATTC 1 cut(s) 266
EcoRII CCWGG 3 cut(s) 35, 324, 352
EcoT14I CCWWGG 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 576
Esp3I CGTCTC 2 cut(s) 489, 632
FaeI CATG 2 cut(s) 56, 286
FaiI YATR 4 cut(s) 54, 284, 525, 626
FatI CATG 2 cut(s) 52, 282
FblI GTMKAC 1 cut(s) 358
Fnu4HI GCNGC 3 cut(s) 21, 70, 345
FokI GGATG 1 cut(s) 558
Fsp4HI GCNGC 3 cut(s) 21, 70, 345
GlaI GCGC 1 cut(s) 150
GluI GCNGC 3 cut(s) 21, 70, 345
HaeIII GGCC 4 cut(s) 72, 106, 161, 582
HapII CCGG 3 cut(s) 107, 121, 158
HhaI GCGC 1 cut(s) 151
Hin1II CATG 2 cut(s) 56, 286
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HinfI GANTC 5 cut(s) 110, 472, 565, 597, 643
HpaII CCGG 3 cut(s) 107, 121, 158
Hpy166II GTNNAC 2 cut(s) 243, 359
Hpy188I TCNGA 5 cut(s) 62, 175, 238, 471, 648
Hpy188III TCNNGA 6 cut(s) 82, 143, 263, 289, 406, 514
Hpy8I GTNNAC 2 cut(s) 243, 359
HpyAV CCTTC 4 cut(s) 59, 176, 567, 658
HpyCH4III ACNGT 3 cut(s) 339, 445, 678
Hsp92II CATG 2 cut(s) 56, 286
HspAI GCGC 1 cut(s) 149
Kzo9I GATC 3 cut(s) 75, 193, 553
LmnI GCTCC 1 cut(s) 123
Lsp1109I GCAGC 1 cut(s) 331
LweI GCATC 1 cut(s) 136
MaeIII GTNAC 1 cut(s) 95
MalI GATC 3 cut(s) 77, 195, 555
MboI GATC 3 cut(s) 75, 193, 553
MboII GAAGA 3 cut(s) 287, 479, 661
MflI RGATCY 1 cut(s) 553
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 7 cut(s) 230, 247, 266, 396, 414, 428, 477
MlyI GAGTC 2 cut(s) 119, 591
MmeI TCCRAC 1 cut(s) 636
MnlI CCTC 9 cut(s) 4, 21, 53, 89, 143, 169, 207, 413, 661
MseI TTAA 1 cut(s) 399
MspA1I CMGCKG 2 cut(s) 69, 344
MspI CCGG 3 cut(s) 107, 121, 158
MspR9I CCNGG 3 cut(s) 37, 326, 354
MvaI CCWGG 3 cut(s) 37, 326, 354
NdeII GATC 3 cut(s) 75, 193, 553
NlaIII CATG 2 cut(s) 56, 286
NlaIV GGNNCC 4 cut(s) 41, 59, 215, 555
PasI CCCWGGG 1 cut(s) 325
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 3 cut(s) 472, 565, 643
PkrI GCNGC 3 cut(s) 22, 71, 346
PleI GAGTC 2 cut(s) 118, 591
PpsI GAGTC 2 cut(s) 118, 591
PshAI GACNNNNGTC 1 cut(s) 258
Psp6I CCWGG 3 cut(s) 35, 324, 352
PspGI CCWGG 3 cut(s) 35, 324, 352
PspN4I GGNNCC 4 cut(s) 41, 59, 215, 555
PspPI GGNCC 2 cut(s) 296, 580
PsuI RGATCY 1 cut(s) 553
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 2 cut(s) 242, 390
RsaNI GTAC 2 cut(s) 241, 389
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 3 cut(s) 21, 70, 345
Sau3AI GATC 3 cut(s) 75, 193, 553
Sau96I GGNCC 2 cut(s) 296, 580
SchI GAGTC 2 cut(s) 119, 591
ScrFI CCNGG 3 cut(s) 37, 326, 354
SduI GDGCHC 1 cut(s) 120
SetI ASST 8 cut(s) 41, 135, 180, 301, 346, 394, 437, 632
SfaNI GCATC 1 cut(s) 136
SinI GGWCC 1 cut(s) 296
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 7 cut(s) 230, 247, 266, 396, 414, 428, 477
SsiI CCGC 2 cut(s) 21, 69
StyD4I CCNGG 3 cut(s) 35, 324, 352
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 3 cut(s) 339, 445, 678
TaqI TCGA 7 cut(s) 26, 129, 135, 144, 180, 192, 600
TasI AATT 7 cut(s) 230, 247, 266, 396, 414, 428, 477
TatI WGTACW 1 cut(s) 240
TauI GCSGC 2 cut(s) 23, 72
TfiI GAWTC 3 cut(s) 472, 565, 643
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 1 cut(s) 567
TseI GCWGC 1 cut(s) 344
TspDTI ATGAA 5 cut(s) 41, 259, 557, 618, 622
TspGWI ACGGA 1 cut(s) 471
TspRI CASTG 1 cut(s) 567
VpaK11BI GGWCC 1 cut(s) 296
XapI RAATTY 5 cut(s) 230, 247, 266, 428, 477
XmiI GTMKAC 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.