MD15G1380500.v1.1

Dihydrofolate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008449
Physical Location & Seq
Forward (+)
46817726 .. 46820120
2395 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1380500.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGGGAGCCAAGGAAGCGGAGAAGGCGGCGGGTGTTCGAGGAAGCCCAGGTTTCTGTGCCTCCATGGGTTCCGAACTAGCGGGGAAATAATGAGGAAGCAAGTGGGCAAGTGGCCGGAATCGGTGCTGGAGAAGCTCGACATGGTTTATCTCGATGGGCCCTTTCCGGCCGAAGGTAAATCCGACGTCGACGGCATTTTCGATCCTCCTTATTACGAGTGGTTCCAATTCAACCAGGAATTTTCAGCGTACACGAACTTTGACGAGTGTCTGGAATACATAGAAGATTACATGATTAAACAGGGACCGTTCGATGGCCTTGTTGGTTTTTCCCAGGGAGCAATACTGTCGGCGGGGCTGCCGGGGCTGCAAGCGAAGGGTGTGGCATTGACTAAAGTTCCCAAAATTAAATTCCTGATAATAATCGGAGGGGCGAAATTCAAGTCACCGGCGGTGGCTGAGGATGCTTATTCGTCTCCCATTCAGTGCCCGTCCCTTCACTTTTTAGGAGAGATGGATTTCTTGAAGCCGTACGGGCTTGAACTGTTGGAACACTGTGTAGATGCTTCGGTGATTCATCATCCGAAAGGCCACACTGTTCCGAGACTTGACGAAAAGGGTTTGGATACGATGATGAACTTCATCGACAAGATTCAGAACATATTGGCGGAGAAGGATCAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1

Protein Analysis

229

Amino Acids

25.29

Weight (kDa)

5.15

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 13 - 205 1.8e-54 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 189
AccI GTMKAC 1 cut(s) 189
AciI CCGC 7 cut(s) 18, 27, 30, 81, 353, 452, 668
AclWI GGATC 2 cut(s) 197, 684
AcoI YGGCCR 2 cut(s) 113, 168
AcsI RAATTY 3 cut(s) 239, 410, 437
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 2 cut(s) 251, 533
AfiI CCNNNNNNNGG 2 cut(s) 173, 314
AgsI TTSAA 4 cut(s) 232, 442, 526, 542
AjnI CCWGG 3 cut(s) 47, 234, 333
AjuI GAANNNNNNNTTGG 2 cut(s) 395, 427
AluBI AGCT 1 cut(s) 136
AluI AGCT 1 cut(s) 136
Alw26I GTCTC 2 cut(s) 480, 598
AlwI GGATC 2 cut(s) 197, 684
AoxI GGCC 5 cut(s) 113, 158, 168, 316, 589
ApaI GGGCCC 1 cut(s) 162
ApeKI GCWGC 2 cut(s) 358, 367
ApoI RAATTY 3 cut(s) 239, 410, 437
ArsI GACNNNNNNTTYG 4 cut(s) 182, 214, 428, 460
AspS9I GGNCC 3 cut(s) 158, 159, 305
AsuC2I CCSGG 1 cut(s) 363
AsuHPI GGTGA 2 cut(s) 438, 583
AvaII GGWCC 1 cut(s) 305
BaeGI GKGCMC 2 cut(s) 162, 491
BanII GRGCYC 1 cut(s) 162
BbvCI CCTCAGC 1 cut(s) 459
BbvI GCAGC 2 cut(s) 345, 354
BccI CCATC 3 cut(s) 149, 308, 508
BceAI ACGGC 2 cut(s) 208, 514
BcgI CGANNNNNNTGC 2 cut(s) 330, 364
BciT130I CCWGG 3 cut(s) 49, 236, 335
BciVI GTATCC 1 cut(s) 619
BcnI CCSGG 1 cut(s) 363
BcoDI GTCTC 2 cut(s) 480, 598
BfaI CTAG 1 cut(s) 78
BfuI GTATCC 1 cut(s) 619
BglI GCCNNNNNGGC 1 cut(s) 535
BisI GCNGC 3 cut(s) 28, 359, 368
BlsI GCNGC 3 cut(s) 29, 360, 369
Bme1390I CCNGG 4 cut(s) 49, 236, 335, 363
Bme18I GGWCC 1 cut(s) 305
BmgT120I GGNCC 3 cut(s) 158, 159, 305
BmiI GGNNCC 5 cut(s) 8, 71, 160, 224, 306
BmrFI CCNGG 4 cut(s) 49, 236, 335, 363
BmsI GCATC 2 cut(s) 454, 553
BoxI GACNNNNGTC 1 cut(s) 267
BpmI CTGGAG 1 cut(s) 149
Bpu10I CCTNAGC 1 cut(s) 459
BpuMI CCSGG 1 cut(s) 363
BsaBI GATNNNNATC 1 cut(s) 422
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 6 cut(s) 10, 47, 64, 333, 334, 362
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 314
Bse118I RCCGGY 1 cut(s) 448
Bse8I GATNNNNATC 1 cut(s) 422
BseBI CCWGG 3 cut(s) 49, 236, 335
BseDI CCNNGG 6 cut(s) 10, 47, 64, 333, 334, 362
BseGI GGATG 2 cut(s) 469, 580
BseJI GATNNNNATC 1 cut(s) 422
BseLI CCNNNNNNNGG 2 cut(s) 173, 314
BseMII CTCAG 1 cut(s) 450
BseSI GKGCMC 2 cut(s) 162, 491
BseX3I CGGCCG 1 cut(s) 168
BseXI GCAGC 2 cut(s) 345, 354
Bsh1285I CGRYCG 1 cut(s) 171
BshFI GGCC 5 cut(s) 115, 160, 170, 318, 591
BsiEI CGRYCG 1 cut(s) 171
BsiSI CCGG 4 cut(s) 116, 167, 362, 449
BsiWI CGTACG 1 cut(s) 531
BslFI GGGAC 2 cut(s) 318, 478
BslI CCNNNNNNNGG 2 cut(s) 173, 314
BsmAI GTCTC 2 cut(s) 480, 598
BsmBI CGTCTC 1 cut(s) 480
BsmFI GGGAC 2 cut(s) 318, 478
BsnI GGCC 5 cut(s) 115, 160, 170, 318, 591
Bsp120I GGGCCC 1 cut(s) 158
Bsp1286I GDGCHC 2 cut(s) 162, 491
Bsp143I GATC 2 cut(s) 202, 676
Bsp19I CCATGG 1 cut(s) 64
BspACI CCGC 7 cut(s) 18, 27, 30, 81, 353, 452, 668
BspANI GGCC 5 cut(s) 115, 160, 170, 318, 591
BspCNI CTCAG 1 cut(s) 451
BspLI GGNNCC 5 cut(s) 8, 71, 160, 224, 306
BspPI GGATC 2 cut(s) 197, 684
BsrFI RCCGGY 1 cut(s) 448
BssAI RCCGGY 1 cut(s) 448
BssECI CCNNGG 6 cut(s) 10, 47, 64, 333, 334, 362
BssMI GATC 2 cut(s) 202, 676
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 2 cut(s) 10, 64
Bst2UI CCWGG 3 cut(s) 49, 236, 335
Bst4CI ACNGT 6 cut(s) 309, 348, 546, 557, 598, 684
BstACI GRCGYC 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 1 cut(s) 459
BstDSI CCRYGG 1 cut(s) 64
BstF5I GGATG 2 cut(s) 469, 580
BstKTI GATC 2 cut(s) 205, 679
BstMAI GTCTC 2 cut(s) 480, 598
BstMBI GATC 2 cut(s) 202, 676
BstMCI CGRYCG 1 cut(s) 171
BstMWI GCNNNNNNNGC 7 cut(s) 15, 24, 133, 364, 367, 464, 535
BstNI CCWGG 3 cut(s) 49, 236, 335
BstPAI GACNNNNGTC 1 cut(s) 267
BstSCI CCNGG 4 cut(s) 47, 234, 333, 361
BstSLI GKGCMC 2 cut(s) 162, 491
BstV1I GCAGC 2 cut(s) 345, 354
BstZI CGGCCG 1 cut(s) 168
BsuI GTATCC 1 cut(s) 619
BsuRI GGCC 5 cut(s) 115, 160, 170, 318, 591
BtgI CCRYGG 1 cut(s) 64
BtsCI GGATG 2 cut(s) 469, 580
BtsIMutI CAGTG 3 cut(s) 491, 553, 594
Cac8I GCNNGC 1 cut(s) 372
Cfr10I RCCGGY 1 cut(s) 448
Cfr13I GGNCC 3 cut(s) 158, 159, 305
Csp6I GTAC 2 cut(s) 250, 532
CviAII CATG 3 cut(s) 65, 142, 292
CviQI GTAC 2 cut(s) 250, 532
DdeI CTNAG 1 cut(s) 459
DpnI GATC 2 cut(s) 204, 678
DpnII GATC 2 cut(s) 202, 676
EaeI YGGCCR 2 cut(s) 113, 168
EagI CGGCCG 1 cut(s) 168
EciI GGCGGA 1 cut(s) 683
EclXI CGGCCG 1 cut(s) 168
Eco130I CCWWGG 2 cut(s) 10, 64
Eco24I GRGCYC 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 305
Eco52I CGGCCG 1 cut(s) 168
EcoO109I RGGNCCY 1 cut(s) 159
EcoRII CCWGG 3 cut(s) 47, 234, 333
EcoT14I CCWWGG 2 cut(s) 10, 64
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 2 cut(s) 10, 64
Esp3I CGTCTC 1 cut(s) 480
FaeI CATG 3 cut(s) 68, 145, 295
FaiI YATR 5 cut(s) 66, 143, 281, 293, 662
FaqI GGGAC 2 cut(s) 318, 478
FatI CATG 3 cut(s) 64, 141, 291
FauI CCCGC 3 cut(s) 23, 74, 346
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 3 cut(s) 28, 359, 368
FokI GGATG 2 cut(s) 476, 567
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 3 cut(s) 28, 359, 368
FspBI CTAG 1 cut(s) 78
GluI GCNGC 3 cut(s) 28, 359, 368
GsuI CTGGAG 1 cut(s) 149
HaeIII GGCC 5 cut(s) 115, 160, 170, 318, 591
HapII CCGG 4 cut(s) 116, 167, 362, 449
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 3 cut(s) 68, 145, 295
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 3 cut(s) 119, 574, 652
HpaII CCGG 4 cut(s) 116, 167, 362, 449
HphI GGTGA 2 cut(s) 438, 583
Hpy166II GTNNAC 2 cut(s) 190, 252
Hpy188I TCNGA 6 cut(s) 74, 184, 428, 585, 603, 657
Hpy188III TCNNGA 4 cut(s) 152, 272, 415, 523
Hpy8I GTNNAC 2 cut(s) 190, 252
Hpy99I CGWCG 3 cut(s) 188, 191, 194
HpyAV CCTTC 5 cut(s) 17, 167, 370, 506, 667
HpyCH4III ACNGT 6 cut(s) 309, 348, 546, 557, 598, 684
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 1 cut(s) 370
HpyF10VI GCNNNNNNNGC 7 cut(s) 15, 24, 133, 364, 367, 464, 535
HpyF3I CTNAG 1 cut(s) 459
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 3 cut(s) 68, 145, 295
Kzo9I GATC 2 cut(s) 202, 676
LmnI GCTCC 2 cut(s) 6, 338
Lsp1109I GCAGC 2 cut(s) 345, 354
LweI GCATC 2 cut(s) 454, 553
MaeI CTAG 1 cut(s) 78
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 1 cut(s) 444
MalI GATC 2 cut(s) 204, 678
MboI GATC 2 cut(s) 202, 676
MboII GAAGA 1 cut(s) 296
MhlI GDGCHC 2 cut(s) 162, 491
MluCI AATT 5 cut(s) 227, 239, 405, 410, 437
MmeI TCCRAC 2 cut(s) 207, 528
MnlI CCTC 6 cut(s) 33, 71, 87, 216, 422, 454
MseI TTAA 2 cut(s) 297, 408
MspI CCGG 4 cut(s) 116, 167, 362, 449
MspR9I CCNGG 4 cut(s) 49, 236, 335, 363
MvaI CCWGG 3 cut(s) 49, 236, 335
MwoI GCNNNNNNNGC 7 cut(s) 15, 24, 133, 364, 367, 464, 535
NciI CCSGG 1 cut(s) 363
NcoI CCATGG 1 cut(s) 64
NdeII GATC 2 cut(s) 202, 676
NlaIII CATG 3 cut(s) 68, 145, 295
NlaIV GGNNCC 5 cut(s) 8, 71, 160, 224, 306
NmuCI GTSAC 1 cut(s) 444
PasI CCCWGGG 1 cut(s) 334
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PfeI GAWTC 3 cut(s) 119, 574, 652
Pfl23II CGTACG 1 cut(s) 531
PkrI GCNGC 3 cut(s) 29, 360, 369
PshAI GACNNNNGTC 1 cut(s) 267
Psp6I CCWGG 3 cut(s) 47, 234, 333
PspGI CCWGG 3 cut(s) 47, 234, 333
PspLI CGTACG 1 cut(s) 531
PspN4I GGNNCC 5 cut(s) 8, 71, 160, 224, 306
PspOMI GGGCCC 1 cut(s) 158
PspPI GGNCC 3 cut(s) 158, 159, 305
RsaI GTAC 2 cut(s) 251, 533
RsaNI GTAC 2 cut(s) 250, 532
SalI GTCGAC 1 cut(s) 188
SaqAI TTAA 2 cut(s) 297, 408
SatI GCNGC 3 cut(s) 28, 359, 368
Sau3AI GATC 2 cut(s) 202, 676
Sau96I GGNCC 3 cut(s) 158, 159, 305
ScrFI CCNGG 4 cut(s) 49, 236, 335, 363
SduI GDGCHC 2 cut(s) 162, 491
SetI ASST 4 cut(s) 53, 138, 178, 189
SfaNI GCATC 2 cut(s) 454, 553
SgrAI CRCCGGYG 1 cut(s) 448
SgrDI CGTCGACG 1 cut(s) 188
SinI GGWCC 1 cut(s) 305
Sse9I AATT 5 cut(s) 227, 239, 405, 410, 437
SsiI CCGC 7 cut(s) 18, 27, 30, 81, 353, 452, 668
SspMI CTAG 1 cut(s) 78
StyD4I CCNGG 4 cut(s) 47, 234, 333, 361
StyI CCWWGG 2 cut(s) 10, 64
TaaI ACNGT 6 cut(s) 309, 348, 546, 557, 598, 684
TaiI ACGT 1 cut(s) 189
TaqI TCGA 7 cut(s) 38, 138, 153, 189, 201, 312, 645
TasI AATT 5 cut(s) 227, 239, 405, 410, 437
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 3 cut(s) 119, 574, 652
Tru1I TTAA 2 cut(s) 297, 408
Tru9I TTAA 2 cut(s) 297, 408
TscAI CASTG 3 cut(s) 491, 560, 601
TseFI GTSAC 1 cut(s) 444
TseI GCWGC 2 cut(s) 358, 367
Tsp45I GTSAC 1 cut(s) 444
TspDTI ATGAA 3 cut(s) 566, 631, 650
TspRI CASTG 3 cut(s) 491, 560, 601
VpaK11BI GGWCC 1 cut(s) 305
XapI RAATTY 3 cut(s) 239, 410, 437
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 1 cut(s) 78
ZraI GACGTC 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.