Rroxscaffold_3G00223870

Dihydrofolate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008449
Physical Location & Seq
Forward (+)
6967883 .. 6970061
2179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00223870.1

Sequence Viewer

Length: 960 bp
ATGTCATCCAATGCATCATCCACCCTCCACTTAAACCGCACGCCCATTCTGGACCGTCGATTTCCTCTACGCGGCCTACGAACCCTATTTATTGTTCCCCATTCCCTTCGCTCCATTCGCATTGAAAGCAGGAAACTCCAACACGACGTCGTTGCGACCAAGGCCGACGTGCCTTTTAAATCCCCCACCACCTCCCGCACTAGCGGAACTGACAAGAACAACAACAACAACAGCCTCAATCTTTCCGAAACGACAAGGAACACCAACAACAACATGGGAAGCGAGGGTTGTGCCGCGGCTTCTGATTCGAGGAAACCCAGGTTTCTTTGCCTTCATGGGTTCCGAACCAGCGGTGTGATCATGAAGACCCAACTGGGTAAATGGCCGGAGTCGGTGCTCAACAATTTCGACCTGGACTTTCTCGATGGGCCTTTCCCGGCCCAAGGCAAATCCGAAGTCGAGGGCATTTTCGATCCTCCTTATCACGAGTGGTTCCAATTCAACAAGGATTTTTCTGAGTACACGAACTTTGACGAGTGTCTTGAATTCATTGAAGATTACATGATCAAGAATGGACCCTTTGATGGGCTACTTGGCTTTTCCCAGGGGGCAATACTGTCAGCGGCACTGCCGGGTCTACAAGCAAAGGGTTTGGCATTGACCAAAGCACCCAAAATTAAATTCCTCATAGTAATTGGAGGCGCAAAATTCAAATCGTCAACCGTGGCTGAAAATGCTTATTCATCTCCTATTCAAATTCCGTCTCTTCACTTTTTAGGAGAAACAGACTTCTTGAAGCCATATGGACTCGAACTGCTAGAAAACTGTGTAGATCCTACAGTGATTCATCATCCCAAGGGCCACACGGTACCACGACTCGATGAAAAGGGTTTAGAGACCATTATGAGCTTCATTGAGACAATTCAGAAGAAGTTGGACGAGAAGGAGGAGCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1

Protein Analysis

319

Amino Acids

35.61

Weight (kDa)

6.52

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 104 - 295 1.4e-53 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
Acc65I GGTACC 1 cut(s) 868
AccB1I GGYRCC 1 cut(s) 868
AccI GTMKAC 1 cut(s) 637
AccII CGCG 2 cut(s) 72, 296
AciI CCGC 8 cut(s) 37, 72, 196, 204, 294, 296, 351, 623
AclWI GGATC 2 cut(s) 467, 827
AcoI YGGCCR 1 cut(s) 383
AcsI RAATTY 4 cut(s) 545, 680, 707, 756
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 2 cut(s) 521, 870
AfiI CCNNNNNNNGG 4 cut(s) 71, 443, 584, 585
AgsI TTSAA 7 cut(s) 125, 502, 545, 554, 712, 755, 796
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 3 cut(s) 317, 411, 603
AjuI GAANNNNNNNTTGG 2 cut(s) 665, 697
AluBI AGCT 1 cut(s) 909
AluI AGCT 1 cut(s) 909
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 3 cut(s) 768, 890, 911
AlwI GGATC 2 cut(s) 467, 827
AoxI GGCC 6 cut(s) 73, 162, 383, 428, 438, 859
ApoI RAATTY 4 cut(s) 545, 680, 707, 756
ArsI GACNNNNNNTTYG 2 cut(s) 441, 473
Asp718I GGTACC 1 cut(s) 868
AspLEI GCGC 1 cut(s) 704
AspS9I GGNCC 5 cut(s) 52, 428, 439, 575, 859
AsuC2I CCSGG 2 cut(s) 437, 633
AvaII GGWCC 2 cut(s) 52, 575
BanI GGYRCC 1 cut(s) 868
BauI CACGAG 1 cut(s) 485
BbsI GAAGAC 1 cut(s) 371
Bbv12I GWGCWC 1 cut(s) 399
BccI CCATC 2 cut(s) 419, 578
BcgI CGANNNNNNTGC 4 cut(s) 134, 168, 272, 306
BciT130I CCWGG 3 cut(s) 319, 413, 605
BclI TGATCA 2 cut(s) 357, 564
BcnI CCSGG 2 cut(s) 437, 633
BcoDI GTCTC 3 cut(s) 768, 890, 911
BfaI CTAG 2 cut(s) 201, 818
BfmI CTRYAG 1 cut(s) 837
BisI GCNGC 4 cut(s) 73, 294, 297, 624
BlsI GCNGC 4 cut(s) 74, 295, 298, 625
Bme1390I CCNGG 5 cut(s) 319, 413, 437, 605, 633
Bme18I GGWCC 2 cut(s) 52, 575
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 5 cut(s) 52, 428, 439, 575, 859
BmiI GGNNCC 4 cut(s) 341, 494, 577, 870
BmrFI CCNGG 5 cut(s) 319, 413, 437, 605, 633
BmrI ACTGGG 1 cut(s) 383
BmsI GCATC 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 383
BoxI GACNNNNGTC 1 cut(s) 537
BpiI GAAGAC 1 cut(s) 371
BpuMI CCSGG 2 cut(s) 437, 633
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 890
BsaJI CCNNGG 8 cut(s) 159, 294, 317, 442, 603, 604, 723, 855
BsaXI ACNNNNNCTCC 1 cut(s) 941
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 443, 584, 585
Bse1I ACTGG 1 cut(s) 378
BseBI CCWGG 3 cut(s) 319, 413, 605
BseDI CCNNGG 8 cut(s) 159, 294, 317, 442, 603, 604, 723, 855
BseGI GGATG 3 cut(s) 5, 17, 850
BseLI CCNNNNNNNGG 4 cut(s) 71, 443, 584, 585
BseMII CTCAG 1 cut(s) 507
BseNI ACTGG 1 cut(s) 378
Bsh1236I CGCG 2 cut(s) 72, 296
BshFI GGCC 6 cut(s) 75, 164, 385, 430, 440, 861
BshNI GGYRCC 1 cut(s) 868
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 3 cut(s) 386, 437, 632
BslI CCNNNNNNNGG 4 cut(s) 71, 443, 584, 585
BsmAI GTCTC 3 cut(s) 768, 890, 911
BsmBI CGTCTC 1 cut(s) 768
BsnI GGCC 6 cut(s) 75, 164, 385, 430, 440, 861
Bso31I GGTCTC 1 cut(s) 890
Bsp1286I GDGCHC 1 cut(s) 399
Bsp143I GATC 4 cut(s) 357, 472, 564, 832
BspACI CCGC 8 cut(s) 37, 72, 196, 204, 294, 296, 351, 623
BspANI GGCC 6 cut(s) 75, 164, 385, 430, 440, 861
BspCNI CTCAG 1 cut(s) 508
BspFNI CGCG 2 cut(s) 72, 296
BspHI TCATGA 1 cut(s) 360
BspLI GGNNCC 4 cut(s) 341, 494, 577, 870
BspPI GGATC 2 cut(s) 467, 827
BspT107I GGYRCC 1 cut(s) 868
BspTNI GGTCTC 1 cut(s) 890
BsrI ACTGG 1 cut(s) 378
BssECI CCNNGG 8 cut(s) 159, 294, 317, 442, 603, 604, 723, 855
BssMI GATC 4 cut(s) 357, 472, 564, 832
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 1 cut(s) 485
BssT1I CCWWGG 3 cut(s) 159, 442, 855
Bst2BI CACGAG 1 cut(s) 485
Bst2UI CCWGG 3 cut(s) 319, 413, 605
Bst4CI ACNGT 7 cut(s) 56, 618, 724, 827, 841, 868, 957
Bst6I CTCTTC 1 cut(s) 771
BstACI GRCGYC 1 cut(s) 147
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 1 cut(s) 516
BstDSI CCRYGG 2 cut(s) 294, 723
BstF5I GGATG 3 cut(s) 5, 17, 850
BstFNI CGCG 2 cut(s) 72, 296
BstHHI GCGC 1 cut(s) 704
BstKTI GATC 4 cut(s) 360, 475, 567, 835
BstMAI GTCTC 3 cut(s) 768, 890, 911
BstMBI GATC 4 cut(s) 357, 472, 564, 832
BstMWI GCNNNNNNNGC 4 cut(s) 117, 126, 161, 734
BstNI CCWGG 3 cut(s) 319, 413, 605
BstPAI GACNNNNGTC 1 cut(s) 537
BstSCI CCNGG 5 cut(s) 317, 411, 435, 603, 631
BstSFI CTRYAG 1 cut(s) 837
BstUI CGCG 2 cut(s) 72, 296
BstV2I GAAGAC 1 cut(s) 371
BstX2I RGATCY 1 cut(s) 832
BstYI RGATCY 1 cut(s) 832
BsuRI GGCC 6 cut(s) 75, 164, 385, 430, 440, 861
BtgI CCRYGG 2 cut(s) 294, 723
BtrI CACGTC 1 cut(s) 169
BtsCI GGATG 3 cut(s) 5, 17, 850
BtsI GCAGTG 1 cut(s) 626
BtsIMutI CAGTG 2 cut(s) 626, 846
Cac8I GCNNGC 1 cut(s) 41
CciI TCATGA 1 cut(s) 360
CfoI GCGC 1 cut(s) 704
Cfr13I GGNCC 5 cut(s) 52, 428, 439, 575, 859
Cfr42I CCGCGG 1 cut(s) 297
Csp6I GTAC 2 cut(s) 520, 869
CviAII CATG 4 cut(s) 274, 335, 361, 562
CviQI GTAC 2 cut(s) 520, 869
DdeI CTNAG 1 cut(s) 516
DpnI GATC 4 cut(s) 359, 474, 566, 834
DpnII GATC 4 cut(s) 357, 472, 564, 832
DraI TTTAAA 1 cut(s) 178
EaeI YGGCCR 1 cut(s) 383
Eam1104I CTCTTC 1 cut(s) 771
EarI CTCTTC 1 cut(s) 771
Eco130I CCWWGG 3 cut(s) 159, 442, 855
Eco31I GGTCTC 1 cut(s) 890
Eco47I GGWCC 2 cut(s) 52, 575
EcoRI GAATTC 1 cut(s) 545
EcoRII CCWGG 3 cut(s) 317, 411, 603
EcoT14I CCWWGG 3 cut(s) 159, 442, 855
EcoT22I ATGCAT 1 cut(s) 16
ErhI CCWWGG 3 cut(s) 159, 442, 855
Esp3I CGTCTC 1 cut(s) 768
FaeI CATG 4 cut(s) 277, 338, 364, 565
FaiI YATR 8 cut(s) 275, 336, 362, 563, 689, 802, 804, 905
FatI CATG 4 cut(s) 273, 334, 360, 561
FauI CCCGC 1 cut(s) 203
FauNDI CATATG 1 cut(s) 802
FbaI TGATCA 2 cut(s) 357, 564
FblI GTMKAC 1 cut(s) 637
Fnu4HI GCNGC 4 cut(s) 73, 294, 297, 624
FokI GGATG 2 cut(s) 4, 837
Fsp4HI GCNGC 4 cut(s) 73, 294, 297, 624
FspBI CTAG 2 cut(s) 201, 818
GlaI GCGC 1 cut(s) 703
GluI GCNGC 4 cut(s) 73, 294, 297, 624
HaeIII GGCC 6 cut(s) 75, 164, 385, 430, 440, 861
HapII CCGG 3 cut(s) 386, 437, 632
HhaI GCGC 1 cut(s) 704
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 4 cut(s) 277, 338, 364, 565
Hin6I GCGC 1 cut(s) 702
HinP1I GCGC 1 cut(s) 702
HincII GTYRAC 1 cut(s) 720
HindII GTYRAC 1 cut(s) 720
HinfI GANTC 5 cut(s) 305, 389, 807, 844, 876
HpaII CCGG 3 cut(s) 386, 437, 632
Hpy166II GTNNAC 3 cut(s) 522, 638, 720
Hpy188I TCNGA 6 cut(s) 247, 304, 344, 454, 517, 927
Hpy188III TCNNGA 7 cut(s) 50, 361, 422, 485, 542, 568, 793
Hpy8I GTNNAC 3 cut(s) 522, 638, 720
Hpy99I CGWCG 4 cut(s) 60, 149, 152, 170
HpyAV CCTTC 3 cut(s) 116, 341, 937
HpyCH4III ACNGT 7 cut(s) 56, 618, 724, 827, 841, 868, 957
HpyCH4IV ACGT 2 cut(s) 147, 168
HpyCH4V TGCA 1 cut(s) 14
HpyF10VI GCNNNNNNNGC 4 cut(s) 117, 126, 161, 734
HpyF3I CTNAG 1 cut(s) 516
HpySE526I ACGT 2 cut(s) 147, 168
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 4 cut(s) 277, 338, 364, 565
HspAI GCGC 1 cut(s) 702
KpnI GGTACC 1 cut(s) 872
Ksp22I TGATCA 2 cut(s) 357, 564
KspI CCGCGG 1 cut(s) 297
Kzo9I GATC 4 cut(s) 357, 472, 564, 832
LmnI GCTCC 2 cut(s) 116, 949
LweI GCATC 1 cut(s) 23
MaeI CTAG 2 cut(s) 201, 818
MaeII ACGT 2 cut(s) 147, 168
MalI GATC 4 cut(s) 359, 474, 566, 834
MboI GATC 4 cut(s) 357, 472, 564, 832
MboII GAAGA 4 cut(s) 376, 566, 758, 940
MflI RGATCY 1 cut(s) 832
MhlI GDGCHC 1 cut(s) 399
MluCI AATT 9 cut(s) 403, 497, 545, 675, 680, 693, 707, 756, 921
MlyI GAGTC 3 cut(s) 398, 801, 870
MmeI TCCRAC 2 cut(s) 163, 915
Mph1103I ATGCAT 1 cut(s) 16
MseI TTAA 3 cut(s) 32, 177, 678
MspA1I CMGCKG 3 cut(s) 296, 351, 623
MspI CCGG 3 cut(s) 386, 437, 632
MspR9I CCNGG 5 cut(s) 319, 413, 437, 605, 633
MvaI CCWGG 3 cut(s) 319, 413, 605
MvnI CGCG 2 cut(s) 72, 296
MwoI GCNNNNNNNGC 4 cut(s) 117, 126, 161, 734
NciI CCSGG 2 cut(s) 437, 633
NdeI CATATG 1 cut(s) 802
NdeII GATC 4 cut(s) 357, 472, 564, 832
NlaIII CATG 4 cut(s) 277, 338, 364, 565
NlaIV GGNNCC 4 cut(s) 341, 494, 577, 870
NsiI ATGCAT 1 cut(s) 16
PagI TCATGA 1 cut(s) 360
PasI CCCWGGG 1 cut(s) 604
PcsI WCGNNNNNNNCGW 1 cut(s) 76
PfeI GAWTC 2 cut(s) 305, 844
PkrI GCNGC 4 cut(s) 74, 295, 298, 625
PleI GAGTC 3 cut(s) 397, 801, 870
PpsI GAGTC 3 cut(s) 397, 801, 870
PshAI GACNNNNGTC 1 cut(s) 537
Psp6I CCWGG 3 cut(s) 317, 411, 603
PspGI CCWGG 3 cut(s) 317, 411, 603
PspN4I GGNNCC 4 cut(s) 341, 494, 577, 870
PspPI GGNCC 5 cut(s) 52, 428, 439, 575, 859
PsuI RGATCY 1 cut(s) 832
RsaI GTAC 2 cut(s) 521, 870
RsaNI GTAC 2 cut(s) 520, 869
SacII CCGCGG 1 cut(s) 297
SaqAI TTAA 3 cut(s) 32, 177, 678
SatI GCNGC 4 cut(s) 73, 294, 297, 624
Sau3AI GATC 4 cut(s) 357, 472, 564, 832
Sau96I GGNCC 5 cut(s) 52, 428, 439, 575, 859
SchI GAGTC 3 cut(s) 398, 801, 870
ScrFI CCNGG 5 cut(s) 319, 413, 437, 605, 633
SduI GDGCHC 1 cut(s) 399
SetI ASST 6 cut(s) 150, 171, 194, 323, 414, 911
SfaNI GCATC 1 cut(s) 23
SfcI CTRYAG 1 cut(s) 837
Sfr303I CCGCGG 1 cut(s) 297
SgrBI CCGCGG 1 cut(s) 297
SinI GGWCC 2 cut(s) 52, 575
Sse9I AATT 9 cut(s) 403, 497, 545, 675, 680, 693, 707, 756, 921
SsiI CCGC 8 cut(s) 37, 72, 196, 204, 294, 296, 351, 623
SspMI CTAG 2 cut(s) 201, 818
StyD4I CCNGG 5 cut(s) 317, 411, 435, 603, 631
StyI CCWWGG 3 cut(s) 159, 442, 855
TaaI ACNGT 7 cut(s) 56, 618, 724, 827, 841, 868, 957
TaiI ACGT 2 cut(s) 150, 171
TaqI TCGA 8 cut(s) 58, 308, 408, 423, 459, 471, 810, 879
TasI AATT 9 cut(s) 403, 497, 545, 675, 680, 693, 707, 756, 921
TatI WGTACW 1 cut(s) 519
TauI GCSGC 4 cut(s) 75, 296, 299, 626
TfiI GAWTC 2 cut(s) 305, 844
Tru1I TTAA 3 cut(s) 32, 177, 678
Tru9I TTAA 3 cut(s) 32, 177, 678
TscAI CASTG 2 cut(s) 633, 846
TspDTI ATGAA 7 cut(s) 323, 377, 538, 732, 836, 897, 901
TspGWI ACGGA 1 cut(s) 750
TspRI CASTG 2 cut(s) 633, 846
VpaK11BI GGWCC 2 cut(s) 52, 575
XapI RAATTY 4 cut(s) 545, 680, 707, 756
XcmI CCANNNNNNNNNTGG 1 cut(s) 271
XmiI GTMKAC 1 cut(s) 637
XspI CTAG 2 cut(s) 201, 818
ZraI GACGTC 1 cut(s) 148
Zsp2I ATGCAT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.