MD08G1191700.v1.1

Dihydrofolate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008449
Physical Location & Seq
Forward (+)
24528508 .. 24531402
2895 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1191700.v1.1.491

Sequence Viewer

Length: 933 bp
ATGACGTCATCCCTCGCATCAAACACCGTTTTTCACATGGAACGAGGGAGCGCCCCACACGACGTCGTCAAGGCCCAACTCGCCCTCAAACCTGCACCTTTTAAATCCAACCGAATTCCACTCGCATTCCATCGCTCCTCCCACACTACCGGAGCTCCCGAGAAGAAGAAGAGCAACCTCGTCGACCGATCGGAATATTCTTATTCTCCTTATTATCTCCGAAAAACAACAGAAAAAGAGAACATGGGAAGCCAAGGAAGCCAAGGAGGCGGCGGCGGGTGTTCGAGGAAACCCAGGTTTCTGTGCCTCCATGGGTTCCGAACGAACGGAGAAATCATGAGGAAACAAGTGGGAAAGTGGCCGGAATCGGTGCTGGAGAATTTCGACATGGTTTATCTCGATGCGCCCTTTCCGGCCCAAGGTAAATCCGAAGTTGAAGGCATTTTCGATCCTCCTTATTACGAGTGGTTCCAATTCAACAAGGAATTTTCAGAGTACACAAACTTTGATAAGTGTCTGGAATACATAGAGGATTACATGGTTAAACAGGGGCCGTTCGATGGCCTTGTTGGTTTTTCCCAGGGAGCAGTAATATCGGCTGGGCTGCCGGGGCTGCAAGCGAAGGGTGTGGCATTGACTAAAGTTCCCAAAATTAAATTCCTGATAATAATTGGAGGAGCAAAATTCAAGTCACCGGCAGTGGCTGAGGATGCTTATTCCTCTCCCATTCAGTGCCCCTCCCTTCACTTTTTAGGAGAGATGGACTTCTTGAAGCCGTACGGGCTTGAACTGTTGGAACATTGTGCAGATGCTTCGGTGATTCATCATCCGAAAGGCCACACTGTTCCGAGACTTGACGAAAAGGGTTTAGAGACGATGATGAGCTTCATTGACAACATTCAAAAGATATTGGCAGAGAAAGACCAACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1

Protein Analysis

311

Amino Acids

34.55

Weight (kDa)

7.17

Isoelectric Point (pI)

37.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 95 - 287 1.6e-54 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 8, 66
Acc36I ACCTGC 1 cut(s) 100
AccI GTMKAC 1 cut(s) 183
AciI CCGC 3 cut(s) 270, 273, 276
AclWI GGATC 1 cut(s) 443
AcoI YGGCCR 1 cut(s) 359
AcsI RAATTY 5 cut(s) 114, 379, 485, 656, 683
AcyI GRCGYC 2 cut(s) 5, 63
AfaI GTAC 2 cut(s) 497, 779
AfiI CCNNNNNNNGG 2 cut(s) 419, 560
AgsI TTSAA 6 cut(s) 437, 478, 688, 772, 788, 902
AjnI CCWGG 2 cut(s) 293, 579
AjuI GAANNNNNNNTTGG 2 cut(s) 641, 673
AluBI AGCT 2 cut(s) 155, 885
AluI AGCT 2 cut(s) 155, 885
Alw21I GWGCWC 1 cut(s) 157
Alw26I GTCTC 2 cut(s) 844, 866
AlwI GGATC 1 cut(s) 443
AlwNI CAGNNNCTG 1 cut(s) 704
Ama87I CYCGRG 1 cut(s) 158
AoxI GGCC 6 cut(s) 72, 359, 414, 551, 562, 835
ApeKI GCWGC 2 cut(s) 604, 613
ApoI RAATTY 5 cut(s) 114, 379, 485, 656, 683
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
AspLEI GCGC 2 cut(s) 53, 406
AspS9I GGNCC 3 cut(s) 73, 415, 551
AsuC2I CCSGG 1 cut(s) 609
AsuHPI GGTGA 2 cut(s) 684, 829
AvaI CYCGRG 1 cut(s) 158
BaeGI GKGCMC 1 cut(s) 737
BanII GRGCYC 1 cut(s) 157
Bbv12I GWGCWC 1 cut(s) 157
BbvCI CCTCAGC 1 cut(s) 705
BbvI GCAGC 2 cut(s) 591, 600
BccI CCATC 3 cut(s) 138, 554, 754
BceAI ACGGC 2 cut(s) 538, 760
BcgI CGANNNNNNTGC 6 cut(s) 163, 197, 576, 610, 795, 829
BciT130I CCWGG 2 cut(s) 295, 581
BcnI CCSGG 1 cut(s) 609
BcoDI GTCTC 2 cut(s) 844, 866
BfaI CTAG 1 cut(s) 931
BfoI RGCGCY 1 cut(s) 54
BfuAI ACCTGC 1 cut(s) 100
BglI GCCNNNNNGGC 2 cut(s) 267, 781
BisI GCNGC 4 cut(s) 271, 274, 605, 614
BlsI GCNGC 4 cut(s) 272, 275, 606, 615
Bme1390I CCNGG 3 cut(s) 295, 581, 609
BmeT110I CYCGRG 1 cut(s) 158
BmgT120I GGNCC 3 cut(s) 73, 415, 551
BmiI GGNNCC 3 cut(s) 317, 470, 552
BmrFI CCNGG 3 cut(s) 295, 581, 609
BmsI GCATC 4 cut(s) 26, 391, 700, 799
BpmI CTGGAG 1 cut(s) 395
Bpu10I CCTNAGC 1 cut(s) 705
BpuMI CCSGG 1 cut(s) 609
BsaHI GRCGYC 2 cut(s) 5, 63
BsaJI CCNNGG 8 cut(s) 253, 262, 293, 310, 418, 579, 580, 608
BsaWI WCCGGW 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 139, 169
Bsc4I CCNNNNNNNGG 2 cut(s) 419, 560
Bse118I RCCGGY 1 cut(s) 694
BseBI CCWGG 2 cut(s) 295, 581
BseDI CCNNGG 8 cut(s) 253, 262, 293, 310, 418, 579, 580, 608
BseGI GGATG 3 cut(s) 8, 715, 826
BseLI CCNNNNNNNGG 2 cut(s) 419, 560
BseMII CTCAG 1 cut(s) 696
BseRI GAGGAG 2 cut(s) 127, 690
BseSI GKGCMC 1 cut(s) 737
BseXI GCAGC 2 cut(s) 591, 600
BseYI CCCAGC 1 cut(s) 599
BsgI GTGCAG 2 cut(s) 78, 825
Bsh1285I CGRYCG 2 cut(s) 187, 191
BshFI GGCC 6 cut(s) 74, 361, 416, 553, 564, 837
BsiEI CGRYCG 2 cut(s) 187, 191
BsiHKAI GWGCWC 1 cut(s) 157
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 5 cut(s) 150, 362, 413, 608, 695
BsiWI CGTACG 1 cut(s) 777
BslI CCNNNNNNNGG 2 cut(s) 419, 560
BsmAI GTCTC 2 cut(s) 844, 866
BsmBI CGTCTC 1 cut(s) 866
BsmI GAATGC 1 cut(s) 125
BsnI GGCC 6 cut(s) 74, 361, 416, 553, 564, 837
BsoBI CYCGRG 1 cut(s) 158
Bsp1286I GDGCHC 2 cut(s) 157, 737
Bsp143I GATC 2 cut(s) 188, 448
Bsp19I CCATGG 1 cut(s) 310
BspACI CCGC 3 cut(s) 270, 273, 276
BspANI GGCC 6 cut(s) 74, 361, 416, 553, 564, 837
BspCNI CTCAG 1 cut(s) 697
BspHI TCATGA 1 cut(s) 336
BspLI GGNNCC 3 cut(s) 317, 470, 552
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 1 cut(s) 443
BspQI GCTCTTC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 694
BssAI RCCGGY 1 cut(s) 694
BssECI CCNNGG 8 cut(s) 253, 262, 293, 310, 418, 579, 580, 608
BssMI GATC 2 cut(s) 188, 448
BssNI GRCGYC 2 cut(s) 5, 63
BssT1I CCWWGG 4 cut(s) 253, 262, 310, 418
Bst2UI CCWGG 2 cut(s) 295, 581
Bst4CI ACNGT 3 cut(s) 28, 792, 844
Bst6I CTCTTC 1 cut(s) 164
BstACI GRCGYC 2 cut(s) 5, 63
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 1 cut(s) 705
BstDSI CCRYGG 1 cut(s) 310
BstF5I GGATG 3 cut(s) 8, 715, 826
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 2 cut(s) 53, 406
BstKTI GATC 2 cut(s) 191, 451
BstMAI GTCTC 2 cut(s) 844, 866
BstMBI GATC 2 cut(s) 188, 448
BstMCI CGRYCG 2 cut(s) 187, 191
BstMWI GCNNNNNNNGC 7 cut(s) 80, 258, 267, 610, 613, 710, 781
BstNI CCWGG 2 cut(s) 295, 581
BstSCI CCNGG 3 cut(s) 293, 579, 607
BstSLI GKGCMC 1 cut(s) 737
BstV1I GCAGC 2 cut(s) 591, 600
BsuRI GGCC 6 cut(s) 74, 361, 416, 553, 564, 837
BtgI CCRYGG 1 cut(s) 310
BtgZI GCGATG 1 cut(s) 116
BtsCI GGATG 3 cut(s) 8, 715, 826
BtsI GCAGTG 1 cut(s) 705
BtsIMutI CAGTG 3 cut(s) 705, 737, 840
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 1 cut(s) 618
CaiI CAGNNNCTG 1 cut(s) 704
CciI TCATGA 1 cut(s) 336
CfoI GCGC 2 cut(s) 53, 406
Cfr10I RCCGGY 1 cut(s) 694
Cfr13I GGNCC 3 cut(s) 73, 415, 551
Csp6I GTAC 2 cut(s) 496, 778
CviAII CATG 6 cut(s) 37, 244, 311, 337, 388, 538
CviQI GTAC 2 cut(s) 496, 778
DdeI CTNAG 1 cut(s) 705
DpnI GATC 2 cut(s) 190, 450
DpnII GATC 2 cut(s) 188, 448
DraI TTTAAA 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 359
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
Ecl136II GAGCTC 1 cut(s) 155
Eco130I CCWWGG 4 cut(s) 253, 262, 310, 418
Eco24I GRGCYC 1 cut(s) 157
Eco53kI GAGCTC 1 cut(s) 155
Eco88I CYCGRG 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 155
EcoRI GAATTC 1 cut(s) 114
EcoRII CCWGG 2 cut(s) 293, 579
EcoT14I CCWWGG 4 cut(s) 253, 262, 310, 418
EcoT38I GRGCYC 1 cut(s) 157
ErhI CCWWGG 4 cut(s) 253, 262, 310, 418
Esp3I CGTCTC 1 cut(s) 866
FaeI CATG 6 cut(s) 40, 247, 314, 340, 391, 541
FaiI YATR 7 cut(s) 38, 245, 312, 338, 389, 527, 539
FatI CATG 6 cut(s) 36, 243, 310, 336, 387, 537
FauI CCCGC 1 cut(s) 269
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 4 cut(s) 271, 274, 605, 614
FokI GGATG 2 cut(s) 722, 813
FriOI GRGCYC 1 cut(s) 157
Fsp4HI GCNGC 4 cut(s) 271, 274, 605, 614
FspBI CTAG 1 cut(s) 931
GlaI GCGC 2 cut(s) 52, 405
GluI GCNGC 4 cut(s) 271, 274, 605, 614
GsaI CCCAGC 1 cut(s) 603
GsuI CTGGAG 1 cut(s) 395
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 6 cut(s) 74, 361, 416, 553, 564, 837
HapII CCGG 5 cut(s) 150, 362, 413, 608, 695
HhaI GCGC 2 cut(s) 53, 406
Hin1I GRCGYC 2 cut(s) 5, 63
Hin1II CATG 6 cut(s) 40, 247, 314, 340, 391, 541
Hin6I GCGC 2 cut(s) 51, 404
HinP1I GCGC 2 cut(s) 51, 404
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 2 cut(s) 365, 820
HpaII CCGG 5 cut(s) 150, 362, 413, 608, 695
HphI GGTGA 2 cut(s) 684, 829
Hpy166II GTNNAC 2 cut(s) 184, 498
Hpy188I TCNGA 7 cut(s) 193, 221, 320, 430, 493, 831, 849
Hpy188III TCNNGA 6 cut(s) 158, 337, 398, 518, 661, 769
Hpy8I GTNNAC 2 cut(s) 184, 498
Hpy99I CGWCG 3 cut(s) 65, 68, 185
HpyAV CCTTC 3 cut(s) 431, 616, 752
HpyCH4III ACNGT 3 cut(s) 28, 792, 844
HpyCH4IV ACGT 2 cut(s) 5, 63
HpyCH4V TGCA 3 cut(s) 95, 616, 806
HpyF10VI GCNNNNNNNGC 7 cut(s) 80, 258, 267, 610, 613, 710, 781
HpyF3I CTNAG 1 cut(s) 705
HpySE526I ACGT 2 cut(s) 5, 63
Hsp92I GRCGYC 2 cut(s) 5, 63
Hsp92II CATG 6 cut(s) 40, 247, 314, 340, 391, 541
HspAI GCGC 2 cut(s) 51, 404
Kzo9I GATC 2 cut(s) 188, 448
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 6 cut(s) 48, 140, 152, 160, 584, 677
Lsp1109I GCAGC 2 cut(s) 591, 600
LweI GCATC 4 cut(s) 26, 391, 700, 799
MaeI CTAG 1 cut(s) 931
MaeII ACGT 2 cut(s) 5, 63
MaeIII GTNAC 1 cut(s) 690
MalI GATC 2 cut(s) 190, 450
MboI GATC 2 cut(s) 188, 448
MboII GAAGA 3 cut(s) 175, 178, 181
MhlI GDGCHC 2 cut(s) 157, 737
MluCI AATT 8 cut(s) 114, 379, 473, 485, 651, 656, 669, 683
MmeI TCCRAC 2 cut(s) 132, 774
MseI TTAA 3 cut(s) 102, 543, 654
MspI CCGG 5 cut(s) 150, 362, 413, 608, 695
MspR9I CCNGG 3 cut(s) 295, 581, 609
Mva1269I GAATGC 1 cut(s) 125
MvaI CCWGG 2 cut(s) 295, 581
MwoI GCNNNNNNNGC 7 cut(s) 80, 258, 267, 610, 613, 710, 781
NciI CCSGG 1 cut(s) 609
NcoI CCATGG 1 cut(s) 310
NdeII GATC 2 cut(s) 188, 448
NlaIII CATG 6 cut(s) 40, 247, 314, 340, 391, 541
NlaIV GGNNCC 3 cut(s) 317, 470, 552
NmuCI GTSAC 1 cut(s) 690
PagI TCATGA 1 cut(s) 336
PasI CCCWGGG 1 cut(s) 580
PciSI GCTCTTC 1 cut(s) 164
PctI GAATGC 1 cut(s) 125
PfeI GAWTC 2 cut(s) 365, 820
Pfl23II CGTACG 1 cut(s) 777
PflFI GACNNNGTC 1 cut(s) 65
PkrI GCNGC 4 cut(s) 272, 275, 606, 615
Ple19I CGATCG 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 157
Psp6I CCWGG 2 cut(s) 293, 579
PspFI CCCAGC 1 cut(s) 599
PspGI CCWGG 2 cut(s) 293, 579
PspLI CGTACG 1 cut(s) 777
PspN4I GGNNCC 3 cut(s) 317, 470, 552
PspPI GGNCC 3 cut(s) 73, 415, 551
PstNI CAGNNNCTG 1 cut(s) 704
PsyI GACNNNGTC 1 cut(s) 65
PvuI CGATCG 1 cut(s) 191
RsaI GTAC 2 cut(s) 497, 779
RsaNI GTAC 2 cut(s) 496, 778
SacI GAGCTC 1 cut(s) 157
SalI GTCGAC 1 cut(s) 182
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 3 cut(s) 102, 543, 654
SatI GCNGC 4 cut(s) 271, 274, 605, 614
Sau3AI GATC 2 cut(s) 188, 448
Sau96I GGNCC 3 cut(s) 73, 415, 551
ScrFI CCNGG 3 cut(s) 295, 581, 609
SduI GDGCHC 2 cut(s) 157, 737
SetI ASST 9 cut(s) 8, 66, 94, 100, 157, 180, 299, 424, 887
SfaNI GCATC 4 cut(s) 26, 391, 700, 799
Sse9I AATT 8 cut(s) 114, 379, 473, 485, 651, 656, 669, 683
SsiI CCGC 3 cut(s) 270, 273, 276
SspI AATATT 1 cut(s) 197
SspMI CTAG 1 cut(s) 931
SstI GAGCTC 1 cut(s) 157
StyD4I CCNGG 3 cut(s) 293, 579, 607
StyI CCWWGG 4 cut(s) 253, 262, 310, 418
TaaI ACNGT 3 cut(s) 28, 792, 844
TaiI ACGT 2 cut(s) 8, 66
TaqI TCGA 6 cut(s) 183, 284, 384, 399, 447, 558
TaqII GACCGA 1 cut(s) 201
TasI AATT 8 cut(s) 114, 379, 473, 485, 651, 656, 669, 683
TatI WGTACW 1 cut(s) 495
TauI GCSGC 2 cut(s) 273, 276
TfiI GAWTC 2 cut(s) 365, 820
Tru1I TTAA 3 cut(s) 102, 543, 654
Tru9I TTAA 3 cut(s) 102, 543, 654
TscAI CASTG 3 cut(s) 705, 737, 847
TseFI GTSAC 1 cut(s) 690
TseI GCWGC 2 cut(s) 604, 613
Tsp45I GTSAC 1 cut(s) 690
TspDTI ATGAA 2 cut(s) 812, 877
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 3 cut(s) 705, 737, 847
Tth111I GACNNNGTC 1 cut(s) 65
XapI RAATTY 5 cut(s) 114, 379, 485, 656, 683
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 1 cut(s) 931
ZraI GACGTC 2 cut(s) 6, 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.