pycom08g16520

Dihydrofolate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008449
Physical Location & Seq
Forward (+)
16488264 .. 16490755
2492 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g16520.2

Sequence Viewer

Length: 996 bp
ATGGCGCGCACATGGAGAGAACACACGCGTGGTCAAAGCTTGCGTTCTCAAATTTCCCTGACGATGACGTCATCCCTCGCCTCAAACACCGTTATTCACATGGAACGAGGGAGAGCCCCACACGACGTCGTCAAGGCCAAACTCGCCCTCAAACCTGCACCTGTTAAATCCAACCAAATTCCACACGCATTCCATCGCTCCTCCCACACTACCGGAGCTCCCGAGAAGAAGAAGAACAACCTCGTCGACCGATCGGAATATTCTTATTCTCCTTATTATCTCCAAAAAACAGGAGAAAAAGAGAACATGGGAAGCCAAGGAAGCGAAGGAGGCGGCGGCGGGTGTTCGAGGAAACCCAGGTTTCTGTGCCTCCATGGGTTCCGAACGAACGGAGAAATCATGAGGAAACAAGTGGGAAAGTGGCCGGAATCGGTGCTGGAGAATTTCGACATGGTTTATCTCGATGCGCCCTTTCCGGCCCAAGGTAAATCCGAAGTTGAAGGCATTTTTGATCCTCCTTATTACGAGTGGTTCCAATTCAACAAGGAATTTACAGAGTACACAAACTTTGATAAGTGTCTGGAATACATAGAGGATTACATGATTAAACAGGGACCGTTCGATGGCCTTGTTGGTTTTTCCCAGGGAGCAATAATATCGGCGGGGCTGCCGGGGCTGCAAGCGAAGGGTGTGGCATTGACCAAAGTTCCCAAAATTAAATTCTTGATAATTATTGGAGGAGCAAAATTCAAGTCACAGGCAGTGGTTGACGATGCTTATTCCTCTTCCATTCAGTGCCCCTCCCTTCACTTTTTAGGAGAGATGGACTTCTTGAAACCGTACGGGCTTGAACTGTTGGAACATTGTGAAGATGCTTCGGTGATTCATCATCCGAAAGGCCACACTGTTCCGAGACTTGACGAAAAGGGTTTAGAGACGATGATGAGCTTCATTGACAAGATTCAAAAGATATTGGCAGAGAAAGACCAACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1

Protein Analysis

332

Amino Acids

37.13

Weight (kDa)

8.14

Isoelectric Point (pI)

34.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 67
AatII GACGTC 2 cut(s) 71, 129
Acc36I ACCTGC 1 cut(s) 163
AccI GTMKAC 1 cut(s) 246
AccII CGCG 2 cut(s) 7, 28
AciI CCGC 4 cut(s) 333, 336, 339, 662
AclWI GGATC 1 cut(s) 506
AcoI YGGCCR 1 cut(s) 422
AcsI RAATTY 6 cut(s) 51, 177, 442, 548, 719, 746
AcyI GRCGYC 2 cut(s) 68, 126
AfaI GTAC 2 cut(s) 560, 842
AfiI CCNNNNNNNGG 2 cut(s) 482, 623
AflIII ACRYGT 1 cut(s) 26
AgsI TTSAA 6 cut(s) 500, 541, 751, 835, 851, 965
AjnI CCWGG 2 cut(s) 356, 642
AjuI GAANNNNNNNTTGG 2 cut(s) 704, 736
AleI CACNNNNGTG 1 cut(s) 27
AluBI AGCT 3 cut(s) 39, 218, 948
AluI AGCT 3 cut(s) 39, 218, 948
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 2 cut(s) 907, 929
AlwI GGATC 1 cut(s) 506
Ama87I CYCGRG 1 cut(s) 221
AoxI GGCC 5 cut(s) 135, 422, 477, 625, 898
ApeKI GCWGC 2 cut(s) 667, 676
ApoI RAATTY 6 cut(s) 51, 177, 442, 548, 719, 746
ArsI GACNNNNNNTTYG 2 cut(s) 737, 769
AspLEI GCGC 3 cut(s) 7, 9, 469
AspS9I GGNCC 2 cut(s) 478, 614
AsuC2I CCSGG 1 cut(s) 672
AsuHPI GGTGA 1 cut(s) 892
AvaI CYCGRG 1 cut(s) 221
AvaII GGWCC 1 cut(s) 614
BaeGI GKGCMC 1 cut(s) 800
BanII GRGCYC 2 cut(s) 118, 220
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 2 cut(s) 654, 663
BccI CCATC 3 cut(s) 201, 617, 817
BcgI CGANNNNNNTGC 2 cut(s) 639, 673
BciT130I CCWGG 2 cut(s) 358, 644
BcnI CCSGG 1 cut(s) 672
BcoDI GTCTC 2 cut(s) 907, 929
BfaI CTAG 1 cut(s) 994
BfuAI ACCTGC 1 cut(s) 163
BisI GCNGC 4 cut(s) 334, 337, 668, 677
BlsI GCNGC 4 cut(s) 335, 338, 669, 678
Bme1390I CCNGG 3 cut(s) 358, 644, 672
Bme18I GGWCC 1 cut(s) 614
BmeT110I CYCGRG 1 cut(s) 221
BmgT120I GGNCC 2 cut(s) 478, 614
BmiI GGNNCC 3 cut(s) 380, 533, 615
BmrFI CCNGG 3 cut(s) 358, 644, 672
BmsI GCATC 3 cut(s) 454, 763, 862
BpmI CTGGAG 1 cut(s) 458
BpuMI CCSGG 1 cut(s) 672
BsaHI GRCGYC 2 cut(s) 68, 126
BsaJI CCNNGG 7 cut(s) 316, 356, 373, 481, 642, 643, 671
BsaWI WCCGGW 1 cut(s) 212
BsaXI ACNNNNNCTCC 2 cut(s) 202, 232
Bsc4I CCNNNNNNNGG 2 cut(s) 482, 623
BseBI CCWGG 2 cut(s) 358, 644
BseDI CCNNGG 7 cut(s) 316, 356, 373, 481, 642, 643, 671
BseGI GGATG 2 cut(s) 71, 889
BseLI CCNNNNNNNGG 2 cut(s) 482, 623
BsePI GCGCGC 1 cut(s) 5
BseRI GAGGAG 2 cut(s) 190, 753
BseSI GKGCMC 1 cut(s) 800
BseXI GCAGC 2 cut(s) 654, 663
BsgI GTGCAG 1 cut(s) 141
Bsh1236I CGCG 2 cut(s) 7, 28
Bsh1285I CGRYCG 2 cut(s) 250, 254
BshFI GGCC 5 cut(s) 137, 424, 479, 627, 900
BsiEI CGRYCG 2 cut(s) 250, 254
BsiHKAI GWGCWC 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 4 cut(s) 213, 425, 476, 671
BsiWI CGTACG 1 cut(s) 840
BslFI GGGAC 1 cut(s) 627
BslI CCNNNNNNNGG 2 cut(s) 482, 623
BsmAI GTCTC 2 cut(s) 907, 929
BsmBI CGTCTC 1 cut(s) 929
BsmFI GGGAC 1 cut(s) 627
BsmI GAATGC 1 cut(s) 188
BsnI GGCC 5 cut(s) 137, 424, 479, 627, 900
BsoBI CYCGRG 1 cut(s) 221
Bsp1286I GDGCHC 3 cut(s) 118, 220, 800
Bsp143I GATC 2 cut(s) 251, 511
Bsp19I CCATGG 1 cut(s) 373
BspACI CCGC 4 cut(s) 333, 336, 339, 662
BspANI GGCC 5 cut(s) 137, 424, 479, 627, 900
BspFNI CGCG 2 cut(s) 7, 28
BspHI TCATGA 1 cut(s) 399
BspLI GGNNCC 3 cut(s) 380, 533, 615
BspMI ACCTGC 1 cut(s) 163
BspPI GGATC 1 cut(s) 506
BssECI CCNNGG 7 cut(s) 316, 356, 373, 481, 642, 643, 671
BssHII GCGCGC 1 cut(s) 5
BssMI GATC 2 cut(s) 251, 511
BssNI GRCGYC 2 cut(s) 68, 126
BssT1I CCWWGG 3 cut(s) 316, 373, 481
Bst2UI CCWGG 2 cut(s) 358, 644
Bst4CI ACNGT 5 cut(s) 91, 618, 840, 855, 907
Bst6I CTCTTC 1 cut(s) 790
BstACI GRCGYC 2 cut(s) 68, 126
BstC8I GCNNGC 3 cut(s) 7, 41, 681
BstDSI CCRYGG 1 cut(s) 373
BstF5I GGATG 2 cut(s) 71, 889
BstFNI CGCG 2 cut(s) 7, 28
BstHHI GCGC 3 cut(s) 7, 9, 469
BstKTI GATC 2 cut(s) 254, 514
BstMAI GTCTC 2 cut(s) 907, 929
BstMBI GATC 2 cut(s) 251, 511
BstMCI CGRYCG 2 cut(s) 250, 254
BstMWI GCNNNNNNNGC 5 cut(s) 143, 321, 330, 673, 676
BstNI CCWGG 2 cut(s) 358, 644
BstSCI CCNGG 3 cut(s) 356, 642, 670
BstSLI GKGCMC 1 cut(s) 800
BstUI CGCG 2 cut(s) 7, 28
BstV1I GCAGC 2 cut(s) 654, 663
BsuRI GGCC 5 cut(s) 137, 424, 479, 627, 900
BtgI CCRYGG 1 cut(s) 373
BtgZI GCGATG 1 cut(s) 179
BtsCI GGATG 2 cut(s) 71, 889
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 3 cut(s) 768, 800, 903
BveI ACCTGC 1 cut(s) 163
Cac8I GCNNGC 3 cut(s) 7, 41, 681
CciI TCATGA 1 cut(s) 399
CfoI GCGC 3 cut(s) 7, 9, 469
Cfr13I GGNCC 2 cut(s) 478, 614
Csp6I GTAC 2 cut(s) 559, 841
CviAII CATG 7 cut(s) 12, 100, 307, 374, 400, 451, 601
CviQI GTAC 2 cut(s) 559, 841
DpnI GATC 2 cut(s) 253, 513
DpnII GATC 2 cut(s) 251, 511
DrdI GACNNNNNNGTC 1 cut(s) 67
DseDI GACNNNNNNGTC 1 cut(s) 67
EaeI YGGCCR 1 cut(s) 422
Eam1104I CTCTTC 1 cut(s) 790
EarI CTCTTC 1 cut(s) 790
Ecl136II GAGCTC 1 cut(s) 218
Eco130I CCWWGG 3 cut(s) 316, 373, 481
Eco24I GRGCYC 2 cut(s) 118, 220
Eco47I GGWCC 1 cut(s) 614
Eco53kI GAGCTC 1 cut(s) 218
Eco88I CYCGRG 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 218
EcoRII CCWGG 2 cut(s) 356, 642
EcoT14I CCWWGG 3 cut(s) 316, 373, 481
EcoT38I GRGCYC 2 cut(s) 118, 220
ErhI CCWWGG 3 cut(s) 316, 373, 481
Esp3I CGTCTC 1 cut(s) 929
FaeI CATG 7 cut(s) 15, 103, 310, 377, 403, 454, 604
FaiI YATR 8 cut(s) 13, 101, 308, 375, 401, 452, 590, 602
FaqI GGGAC 1 cut(s) 627
FatI CATG 7 cut(s) 11, 99, 306, 373, 399, 450, 600
FauI CCCGC 2 cut(s) 332, 655
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 4 cut(s) 334, 337, 668, 677
FokI GGATG 2 cut(s) 58, 876
FriOI GRGCYC 2 cut(s) 118, 220
Fsp4HI GCNGC 4 cut(s) 334, 337, 668, 677
FspBI CTAG 1 cut(s) 994
GlaI GCGC 3 cut(s) 6, 8, 468
GluI GCNGC 4 cut(s) 334, 337, 668, 677
GsuI CTGGAG 1 cut(s) 458
HaeIII GGCC 5 cut(s) 137, 424, 479, 627, 900
HapII CCGG 4 cut(s) 213, 425, 476, 671
HhaI GCGC 3 cut(s) 7, 9, 469
Hin1I GRCGYC 2 cut(s) 68, 126
Hin1II CATG 7 cut(s) 15, 103, 310, 377, 403, 454, 604
Hin6I GCGC 3 cut(s) 5, 7, 467
HinP1I GCGC 3 cut(s) 5, 7, 467
HincII GTYRAC 2 cut(s) 247, 769
HindII GTYRAC 2 cut(s) 247, 769
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 3 cut(s) 428, 883, 961
HpaII CCGG 4 cut(s) 213, 425, 476, 671
HphI GGTGA 1 cut(s) 892
Hpy166II GTNNAC 3 cut(s) 247, 561, 769
Hpy188I TCNGA 5 cut(s) 256, 383, 493, 894, 912
Hpy188III TCNNGA 6 cut(s) 221, 400, 461, 581, 724, 832
Hpy8I GTNNAC 3 cut(s) 247, 561, 769
Hpy99I CGWCG 3 cut(s) 128, 131, 248
HpyAV CCTTC 4 cut(s) 320, 494, 679, 815
HpyCH4III ACNGT 5 cut(s) 91, 618, 840, 855, 907
HpyCH4IV ACGT 2 cut(s) 68, 126
HpyCH4V TGCA 2 cut(s) 158, 679
HpyF10VI GCNNNNNNNGC 5 cut(s) 143, 321, 330, 673, 676
HpySE526I ACGT 2 cut(s) 68, 126
Hsp92I GRCGYC 2 cut(s) 68, 126
Hsp92II CATG 7 cut(s) 15, 103, 310, 377, 403, 454, 604
HspAI GCGC 3 cut(s) 5, 7, 467
Kzo9I GATC 2 cut(s) 251, 511
LmnI GCTCC 5 cut(s) 203, 215, 223, 647, 740
Lsp1109I GCAGC 2 cut(s) 654, 663
LweI GCATC 3 cut(s) 454, 763, 862
MaeI CTAG 1 cut(s) 994
MaeII ACGT 2 cut(s) 68, 126
MaeIII GTNAC 1 cut(s) 753
MalI GATC 2 cut(s) 253, 513
MboI GATC 2 cut(s) 251, 511
MboII GAAGA 5 cut(s) 238, 241, 244, 777, 881
MhlI GDGCHC 3 cut(s) 118, 220, 800
MluCI AATT 9 cut(s) 51, 177, 442, 536, 548, 714, 719, 729, 746
MluI ACGCGT 1 cut(s) 26
MmeI TCCRAC 2 cut(s) 195, 837
MseI TTAA 3 cut(s) 165, 606, 717
MslI CAYNNNNRTG 1 cut(s) 27
MspI CCGG 4 cut(s) 213, 425, 476, 671
MspR9I CCNGG 3 cut(s) 358, 644, 672
Mva1269I GAATGC 1 cut(s) 188
MvaI CCWGG 2 cut(s) 358, 644
MvnI CGCG 2 cut(s) 7, 28
MwoI GCNNNNNNNGC 5 cut(s) 143, 321, 330, 673, 676
NciI CCSGG 1 cut(s) 672
NcoI CCATGG 1 cut(s) 373
NdeII GATC 2 cut(s) 251, 511
NlaIII CATG 7 cut(s) 15, 103, 310, 377, 403, 454, 604
NlaIV GGNNCC 3 cut(s) 380, 533, 615
NmuCI GTSAC 1 cut(s) 753
OliI CACNNNNGTG 1 cut(s) 27
PagI TCATGA 1 cut(s) 399
PasI CCCWGGG 1 cut(s) 643
PauI GCGCGC 1 cut(s) 5
PctI GAATGC 1 cut(s) 188
PfeI GAWTC 3 cut(s) 428, 883, 961
Pfl23II CGTACG 1 cut(s) 840
PflFI GACNNNGTC 1 cut(s) 128
PkrI GCNGC 4 cut(s) 335, 338, 669, 678
Ple19I CGATCG 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 220
Psp6I CCWGG 2 cut(s) 356, 642
PspGI CCWGG 2 cut(s) 356, 642
PspLI CGTACG 1 cut(s) 840
PspN4I GGNNCC 3 cut(s) 380, 533, 615
PspPI GGNCC 2 cut(s) 478, 614
PsyI GACNNNGTC 1 cut(s) 128
PteI GCGCGC 1 cut(s) 5
PvuI CGATCG 1 cut(s) 254
RsaI GTAC 2 cut(s) 560, 842
RsaNI GTAC 2 cut(s) 559, 841
RseI CAYNNNNRTG 1 cut(s) 27
SacI GAGCTC 1 cut(s) 220
SalI GTCGAC 1 cut(s) 245
SaqAI TTAA 3 cut(s) 165, 606, 717
SatI GCNGC 4 cut(s) 334, 337, 668, 677
Sau3AI GATC 2 cut(s) 251, 511
Sau96I GGNCC 2 cut(s) 478, 614
ScrFI CCNGG 3 cut(s) 358, 644, 672
SduI GDGCHC 3 cut(s) 118, 220, 800
SfaNI GCATC 3 cut(s) 454, 763, 862
SinI GGWCC 1 cut(s) 614
SmiMI CAYNNNNRTG 1 cut(s) 27
Sse9I AATT 9 cut(s) 51, 177, 442, 536, 548, 714, 719, 729, 746
SsiI CCGC 4 cut(s) 333, 336, 339, 662
SspI AATATT 1 cut(s) 260
SspMI CTAG 1 cut(s) 994
SstI GAGCTC 1 cut(s) 220
StyD4I CCNGG 3 cut(s) 356, 642, 670
StyI CCWWGG 3 cut(s) 316, 373, 481
TaaI ACNGT 5 cut(s) 91, 618, 840, 855, 907
TaiI ACGT 2 cut(s) 71, 129
TaqI TCGA 5 cut(s) 246, 347, 447, 462, 621
TaqII GACCGA 1 cut(s) 264
TasI AATT 9 cut(s) 51, 177, 442, 536, 548, 714, 719, 729, 746
TatI WGTACW 1 cut(s) 558
TauI GCSGC 2 cut(s) 336, 339
TfiI GAWTC 3 cut(s) 428, 883, 961
Tru1I TTAA 3 cut(s) 165, 606, 717
Tru9I TTAA 3 cut(s) 165, 606, 717
TscAI CASTG 3 cut(s) 768, 800, 910
TseFI GTSAC 1 cut(s) 753
TseI GCWGC 2 cut(s) 667, 676
Tsp45I GTSAC 1 cut(s) 753
TspDTI ATGAA 2 cut(s) 875, 940
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 3 cut(s) 768, 800, 910
Tth111I GACNNNGTC 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 614
XapI RAATTY 6 cut(s) 51, 177, 442, 548, 719, 746
XmiI GTMKAC 1 cut(s) 246
XspI CTAG 1 cut(s) 994
ZraI GACGTC 2 cut(s) 69, 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.