RLG00000000976

Dihydrofolate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008449
Physical Location & Seq
Forward (+)
8745460 .. 8747349
1890 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000976

Sequence Viewer

Length: 957 bp
ATGTCATCCAACGCATCATCCACCCTCCACTTAAACCGCACGCCCATTCTGGACCGTCGATTTCCGCTACGCGGTCTACCTACCCTATTTATTGTTCCCCATTCCCTTCACTCCATTCGCATTGGAAGCAGGAAAGTCCAACACGACGTCGTTGCGACCAAGGCCGACGTGCCTTATAAATCCCCCGCCACCTCCCGCACTAGTGGAACTGACAAGAACAACAACAGCCTCAACCTTTCCGAAACGACAAGGTACACCAACAACAACATGGGAAGCCAGGGTTGTGCCGCGGCGGCTTCTGATTCGAGGAAACCCAGGTTTCTTTGCCTTCATGGGTTTCGAACCAGCGGTGTGATCATGAAGACCCAACTGGGTAAATGGCCGGAGTCGGTGCTCAACAATTTCGACCTGGACTTTCTCGATGGGCCTTTCCCGGCCCAAGGTAAATCCGAAGTCGAGGGCATTTTCGATCCTCCTTATCACGAGTGGTTCCAATTCAACAAGGATTTTTCTGAGTACACGAACTTTGACGAGTGTCTTGAATTCATTGAAGATTACATGATCAAGAATGGACCCTTTGATGGGCTACTTGGCTTTTCCCAGGGGGCAATACTGTCAGCGGCACTGCCGGGTCTACAAGCAAAGGGTTTGGCATTCACCAAAGTACCCAAAATTAAATTCCTCATAATAATTGGAGGCGCAAAATTCAAATCGCCAACCGTGGCTGAAAATGCTTATTCATCTCCTATTCAAATTCCGTCTCTTCACTTTTTAGGAGAAACAGACTTCTTGAAGCCATATGGACTCGAGCTGCTAGAAAACTGTGTAGATCCTACCGTGATTCATCATCCCAAGGGCCACACGATACCACGACTCGATGAAAAGGGTTTAGAGACCATTATGAGCTTCATTGAGACAATTCAGAAGAAGTTGGACGAGAAGGAGGAGCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1

Protein Analysis

319

Amino Acids

35.42

Weight (kDa)

6.56

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 103 - 294 3.8e-53 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AatII GACGTC 1 cut(s) 150
AccI GTMKAC 2 cut(s) 76, 634
AccII CGCG 2 cut(s) 72, 290
AclWI GGATC 2 cut(s) 464, 824
AcoI YGGCCR 1 cut(s) 380
AcsI RAATTY 4 cut(s) 542, 677, 704, 753
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 3 cut(s) 254, 518, 666
AfiI CCNNNNNNNGG 4 cut(s) 71, 440, 581, 582
AgsI TTSAA 6 cut(s) 499, 542, 551, 709, 752, 793
AhlI ACTAGT 1 cut(s) 200
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 4 cut(s) 276, 314, 408, 600
AjuI GAANNNNNNNTTGG 2 cut(s) 662, 694
AluBI AGCT 2 cut(s) 811, 906
AluI AGCT 2 cut(s) 811, 906
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 3 cut(s) 765, 887, 908
AlwI GGATC 2 cut(s) 464, 824
Ama87I CYCGRG 1 cut(s) 806
AoxI GGCC 5 cut(s) 162, 380, 425, 435, 856
ApeKI GCWGC 1 cut(s) 811
ApoI RAATTY 4 cut(s) 542, 677, 704, 753
AspLEI GCGC 1 cut(s) 701
AspS9I GGNCC 5 cut(s) 52, 425, 436, 572, 856
AsuC2I CCSGG 2 cut(s) 434, 630
AsuHPI GGTGA 1 cut(s) 649
AsuII TTCGAA 1 cut(s) 340
AvaI CYCGRG 1 cut(s) 806
AvaII GGWCC 2 cut(s) 52, 572
BaeI ACNNNNGTAYC 2 cut(s) 648, 681
BauI CACGAG 1 cut(s) 482
BbsI GAAGAC 1 cut(s) 368
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 1 cut(s) 798
BccI CCATC 2 cut(s) 416, 575
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 4 cut(s) 278, 316, 410, 602
BclI TGATCA 2 cut(s) 354, 561
BcnI CCSGG 2 cut(s) 434, 630
BcoDI GTCTC 3 cut(s) 765, 887, 908
BcuI ACTAGT 1 cut(s) 200
BfaI CTAG 2 cut(s) 201, 815
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 5 cut(s) 288, 291, 294, 621, 812
BlsI GCNGC 5 cut(s) 289, 292, 295, 622, 813
Bme1390I CCNGG 6 cut(s) 278, 316, 410, 434, 602, 630
Bme18I GGWCC 2 cut(s) 52, 572
BmeT110I CYCGRG 1 cut(s) 806
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 5 cut(s) 52, 425, 436, 572, 856
BmiI GGNNCC 2 cut(s) 491, 574
BmrFI CCNGG 6 cut(s) 278, 316, 410, 434, 602, 630
BmrI ACTGGG 1 cut(s) 380
BmsI GCATC 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 380
BoxI GACNNNNGTC 1 cut(s) 534
BpiI GAAGAC 1 cut(s) 368
Bpu14I TTCGAA 1 cut(s) 340
BpuMI CCSGG 2 cut(s) 434, 630
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 887
BsaJI CCNNGG 9 cut(s) 159, 277, 288, 314, 439, 600, 601, 720, 852
BsaXI ACNNNNNCTCC 1 cut(s) 938
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 440, 581, 582
Bse1I ACTGG 1 cut(s) 375
BseBI CCWGG 4 cut(s) 278, 316, 410, 602
BseDI CCNNGG 9 cut(s) 159, 277, 288, 314, 439, 600, 601, 720, 852
BseGI GGATG 3 cut(s) 5, 17, 847
BseLI CCNNNNNNNGG 4 cut(s) 71, 440, 581, 582
BseMII CTCAG 1 cut(s) 504
BseNI ACTGG 1 cut(s) 375
BseXI GCAGC 1 cut(s) 798
Bsh1236I CGCG 2 cut(s) 72, 290
BshFI GGCC 5 cut(s) 164, 382, 427, 437, 858
BsiHKAI GWGCWC 1 cut(s) 396
BsiHKCI CYCGRG 1 cut(s) 806
BsiSI CCGG 3 cut(s) 383, 434, 629
BslI CCNNNNNNNGG 4 cut(s) 71, 440, 581, 582
BsmAI GTCTC 3 cut(s) 765, 887, 908
BsmBI CGTCTC 1 cut(s) 765
BsmI GAATGC 1 cut(s) 653
BsnI GGCC 5 cut(s) 164, 382, 427, 437, 858
Bso31I GGTCTC 1 cut(s) 887
BsoBI CYCGRG 1 cut(s) 806
Bsp119I TTCGAA 1 cut(s) 340
Bsp1286I GDGCHC 1 cut(s) 396
Bsp143I GATC 4 cut(s) 354, 469, 561, 829
BspANI GGCC 5 cut(s) 164, 382, 427, 437, 858
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 2 cut(s) 72, 290
BspHI TCATGA 1 cut(s) 357
BspLI GGNNCC 2 cut(s) 491, 574
BspPI GGATC 2 cut(s) 464, 824
BspT104I TTCGAA 1 cut(s) 340
BspTNI GGTCTC 1 cut(s) 887
BsrI ACTGG 1 cut(s) 375
BssECI CCNNGG 9 cut(s) 159, 277, 288, 314, 439, 600, 601, 720, 852
BssMI GATC 4 cut(s) 354, 469, 561, 829
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 1 cut(s) 482
BssT1I CCWWGG 3 cut(s) 159, 439, 852
Bst2BI CACGAG 1 cut(s) 482
Bst2UI CCWGG 4 cut(s) 278, 316, 410, 602
Bst4CI ACNGT 6 cut(s) 56, 615, 721, 824, 838, 954
Bst6I CTCTTC 1 cut(s) 768
BstACI GRCGYC 1 cut(s) 147
BstBI TTCGAA 1 cut(s) 340
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 1 cut(s) 513
BstDSI CCRYGG 2 cut(s) 288, 720
BstF5I GGATG 3 cut(s) 5, 17, 847
BstFNI CGCG 2 cut(s) 72, 290
BstHHI GCGC 1 cut(s) 701
BstKTI GATC 4 cut(s) 357, 472, 564, 832
BstMAI GTCTC 3 cut(s) 765, 887, 908
BstMBI GATC 4 cut(s) 354, 469, 561, 829
BstMWI GCNNNNNNNGC 4 cut(s) 126, 161, 293, 731
BstNI CCWGG 4 cut(s) 278, 316, 410, 602
BstPAI GACNNNNGTC 1 cut(s) 534
BstSCI CCNGG 6 cut(s) 276, 314, 408, 432, 600, 628
BstUI CGCG 2 cut(s) 72, 290
BstV1I GCAGC 1 cut(s) 798
BstV2I GAAGAC 1 cut(s) 368
BstX2I RGATCY 1 cut(s) 829
BstYI RGATCY 1 cut(s) 829
BsuRI GGCC 5 cut(s) 164, 382, 427, 437, 858
BtgI CCRYGG 2 cut(s) 288, 720
BtrI CACGTC 1 cut(s) 169
BtsCI GGATG 3 cut(s) 5, 17, 847
BtsI GCAGTG 1 cut(s) 623
BtsIMutI CAGTG 1 cut(s) 623
Cac8I GCNNGC 1 cut(s) 41
CciI TCATGA 1 cut(s) 357
CfoI GCGC 1 cut(s) 701
Cfr13I GGNCC 5 cut(s) 52, 425, 436, 572, 856
Cfr42I CCGCGG 1 cut(s) 291
Csp6I GTAC 3 cut(s) 253, 517, 665
CviAII CATG 4 cut(s) 268, 332, 358, 559
CviQI GTAC 3 cut(s) 253, 517, 665
DdeI CTNAG 1 cut(s) 513
DpnI GATC 4 cut(s) 356, 471, 563, 831
DpnII GATC 4 cut(s) 354, 469, 561, 829
EaeI YGGCCR 1 cut(s) 380
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Eco130I CCWWGG 3 cut(s) 159, 439, 852
Eco31I GGTCTC 1 cut(s) 887
Eco47I GGWCC 2 cut(s) 52, 572
Eco88I CYCGRG 1 cut(s) 806
EcoRI GAATTC 1 cut(s) 542
EcoRII CCWGG 4 cut(s) 276, 314, 408, 600
EcoT14I CCWWGG 3 cut(s) 159, 439, 852
ErhI CCWWGG 3 cut(s) 159, 439, 852
Esp3I CGTCTC 1 cut(s) 765
FaeI CATG 4 cut(s) 271, 335, 361, 562
FaiI YATR 9 cut(s) 177, 269, 333, 359, 560, 686, 799, 801, 902
FatI CATG 4 cut(s) 267, 331, 357, 558
FauI CCCGC 2 cut(s) 193, 203
FauNDI CATATG 1 cut(s) 799
FbaI TGATCA 2 cut(s) 354, 561
FblI GTMKAC 2 cut(s) 76, 634
Fnu4HI GCNGC 5 cut(s) 288, 291, 294, 621, 812
FokI GGATG 2 cut(s) 4, 834
Fsp4HI GCNGC 5 cut(s) 288, 291, 294, 621, 812
FspBI CTAG 2 cut(s) 201, 815
GlaI GCGC 1 cut(s) 700
GluI GCNGC 5 cut(s) 288, 291, 294, 621, 812
HaeIII GGCC 5 cut(s) 164, 382, 427, 437, 858
HapII CCGG 3 cut(s) 383, 434, 629
HhaI GCGC 1 cut(s) 701
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 4 cut(s) 271, 335, 361, 562
Hin6I GCGC 1 cut(s) 699
HinP1I GCGC 1 cut(s) 699
HinfI GANTC 5 cut(s) 302, 386, 804, 841, 873
HpaII CCGG 3 cut(s) 383, 434, 629
HphI GGTGA 1 cut(s) 649
Hpy166II GTNNAC 4 cut(s) 77, 255, 519, 635
Hpy188I TCNGA 5 cut(s) 241, 301, 451, 514, 924
Hpy188III TCNNGA 7 cut(s) 50, 358, 419, 482, 539, 565, 790
Hpy8I GTNNAC 4 cut(s) 77, 255, 519, 635
Hpy99I CGWCG 4 cut(s) 60, 149, 152, 170
HpyAV CCTTC 3 cut(s) 116, 338, 934
HpyCH4III ACNGT 6 cut(s) 56, 615, 721, 824, 838, 954
HpyCH4IV ACGT 2 cut(s) 147, 168
HpyF10VI GCNNNNNNNGC 4 cut(s) 126, 161, 293, 731
HpyF3I CTNAG 1 cut(s) 513
HpySE526I ACGT 2 cut(s) 147, 168
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 4 cut(s) 271, 335, 361, 562
HspAI GCGC 1 cut(s) 699
Ksp22I TGATCA 2 cut(s) 354, 561
KspI CCGCGG 1 cut(s) 291
Kzo9I GATC 4 cut(s) 354, 469, 561, 829
LmnI GCTCC 1 cut(s) 946
Lsp1109I GCAGC 1 cut(s) 798
LweI GCATC 1 cut(s) 23
MaeI CTAG 2 cut(s) 201, 815
MaeII ACGT 2 cut(s) 147, 168
MalI GATC 4 cut(s) 356, 471, 563, 831
MboI GATC 4 cut(s) 354, 469, 561, 829
MboII GAAGA 4 cut(s) 373, 563, 755, 937
MflI RGATCY 1 cut(s) 829
MhlI GDGCHC 1 cut(s) 396
MluCI AATT 9 cut(s) 400, 494, 542, 672, 677, 690, 704, 753, 918
MlyI GAGTC 3 cut(s) 395, 798, 867
MmeI TCCRAC 3 cut(s) 33, 163, 912
MnlI CCTC 9 cut(s) 35, 202, 239, 300, 451, 483, 689, 692, 937
MseI TTAA 2 cut(s) 32, 675
MspA1I CMGCKG 3 cut(s) 290, 348, 620
MspI CCGG 3 cut(s) 383, 434, 629
MspR9I CCNGG 6 cut(s) 278, 316, 410, 434, 602, 630
Mva1269I GAATGC 1 cut(s) 653
MvaI CCWGG 4 cut(s) 278, 316, 410, 602
MvnI CGCG 2 cut(s) 72, 290
MwoI GCNNNNNNNGC 4 cut(s) 126, 161, 293, 731
NciI CCSGG 2 cut(s) 434, 630
NdeI CATATG 1 cut(s) 799
NdeII GATC 4 cut(s) 354, 469, 561, 829
NlaIII CATG 4 cut(s) 271, 335, 361, 562
NlaIV GGNNCC 2 cut(s) 491, 574
NspV TTCGAA 1 cut(s) 340
PaeR7I CTCGAG 1 cut(s) 806
PagI TCATGA 1 cut(s) 357
PasI CCCWGGG 1 cut(s) 601
PctI GAATGC 1 cut(s) 653
PfeI GAWTC 2 cut(s) 302, 841
PkrI GCNGC 5 cut(s) 289, 292, 295, 622, 813
PleI GAGTC 3 cut(s) 394, 798, 867
PpsI GAGTC 3 cut(s) 394, 798, 867
PshAI GACNNNNGTC 1 cut(s) 534
PsiI TTATAA 1 cut(s) 177
Psp6I CCWGG 4 cut(s) 276, 314, 408, 600
PspGI CCWGG 4 cut(s) 276, 314, 408, 600
PspN4I GGNNCC 2 cut(s) 491, 574
PspPI GGNCC 5 cut(s) 52, 425, 436, 572, 856
PspXI VCTCGAGB 1 cut(s) 806
PsuI RGATCY 1 cut(s) 829
RsaI GTAC 3 cut(s) 254, 518, 666
RsaNI GTAC 3 cut(s) 253, 517, 665
SacII CCGCGG 1 cut(s) 291
SaqAI TTAA 2 cut(s) 32, 675
SatI GCNGC 5 cut(s) 288, 291, 294, 621, 812
Sau3AI GATC 4 cut(s) 354, 469, 561, 829
Sau96I GGNCC 5 cut(s) 52, 425, 436, 572, 856
SchI GAGTC 3 cut(s) 395, 798, 867
ScrFI CCNGG 6 cut(s) 278, 316, 410, 434, 602, 630
SduI GDGCHC 1 cut(s) 396
SfaNI GCATC 1 cut(s) 23
Sfr274I CTCGAG 1 cut(s) 806
Sfr303I CCGCGG 1 cut(s) 291
SfuI TTCGAA 1 cut(s) 340
SgrBI CCGCGG 1 cut(s) 291
SinI GGWCC 2 cut(s) 52, 572
SlaI CTCGAG 1 cut(s) 806
SmlI CTYRAG 1 cut(s) 806
SmoI CTYRAG 1 cut(s) 806
SpeI ACTAGT 1 cut(s) 200
Sse9I AATT 9 cut(s) 400, 494, 542, 672, 677, 690, 704, 753, 918
SspMI CTAG 2 cut(s) 201, 815
StyD4I CCNGG 6 cut(s) 276, 314, 408, 432, 600, 628
StyI CCWWGG 3 cut(s) 159, 439, 852
TaaI ACNGT 6 cut(s) 56, 615, 721, 824, 838, 954
TaiI ACGT 2 cut(s) 150, 171
TaqI TCGA 9 cut(s) 58, 305, 340, 405, 420, 456, 468, 807, 876
TasI AATT 9 cut(s) 400, 494, 542, 672, 677, 690, 704, 753, 918
TatI WGTACW 1 cut(s) 516
TauI GCSGC 4 cut(s) 290, 293, 296, 623
TfiI GAWTC 2 cut(s) 302, 841
Tru1I TTAA 2 cut(s) 32, 675
Tru9I TTAA 2 cut(s) 32, 675
TscAI CASTG 1 cut(s) 630
TseI GCWGC 1 cut(s) 811
TspDTI ATGAA 7 cut(s) 320, 374, 535, 729, 833, 894, 898
TspGWI ACGGA 1 cut(s) 747
TspRI CASTG 1 cut(s) 630
VpaK11BI GGWCC 2 cut(s) 52, 572
XapI RAATTY 4 cut(s) 542, 677, 704, 753
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XhoI CTCGAG 1 cut(s) 806
XmiI GTMKAC 2 cut(s) 76, 634
XspI CTAG 2 cut(s) 201, 815
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.