Rw7G038300

Dihydrofolate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0008449
Physical Location & Seq
Reverse (-)
58381237 .. 58382845
1609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G038300.1

Sequence Viewer

Length: 687 bp
ATGGGAAGCGAGGGTTGTGCCGCGGCTTCTGATTCGAGGAAACCCAGGTTTCTTTGCCTTCATGGGTTTCGAACCAGCGGTGTGATCATGAAGACCCAACTGGGTAAATGGCCGGAGTCGGTGCTCAACAATTTCGACCTGGACTTTCTCGATGGGCCTTTCCCGGCCCAAGGTAAATCCGAAGTCGAGGGCATTTTCGATCCTCCTTATCACGAGTGGTTCCAATTCAGCAAGGATTTTTCTGAGTACACGAACTTTGACGAGTGTCTTGAATTCATTGAAGATTACATGATCAAGAATGGACCCTTTGATGGGCTACTTGGCTTTTCCCAGGGGGCAATACTGTCAGCGGCACTGCCGGGTCTACAAGCAAAGGGTTTGGCACTGACCAAAGTACCTAAAATTAAATTCCTCGTAATAATTGGAGGCGCAAAATTCAAGTCAGCAACCGTGGCTGAAAATGCTTATTCATCTCCTATTCAAATTCCGTCTCTTCACTTTTTAGGAGAAACAGACTTCTTGAAGCCATATGGACTCGAACTGCTAGAAAACTGTGTAGATCCTACCGTGATTCATCATCCCAAGGGCCACACGATACCACGACTCGATGAAAAGGGTTTAGAGACCATTATGAGCTTCATTGAGACAATTCAGAAGAAGTTGGACGAGAAGGAAGAGCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1

Protein Analysis

228

Amino Acids

25.38

Weight (kDa)

5.09

Isoelectric Point (pI)

31.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 13 - 204 1.7e-53 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 364
AccII CGCG 1 cut(s) 23
AciI CCGC 4 cut(s) 21, 23, 78, 350
AclWI GGATC 2 cut(s) 194, 554
AcoI YGGCCR 1 cut(s) 110
AcsI RAATTY 4 cut(s) 272, 407, 434, 483
AfaI GTAC 2 cut(s) 248, 396
AfiI CCNNNNNNNGG 3 cut(s) 170, 311, 312
AgsI TTSAA 5 cut(s) 272, 281, 439, 482, 523
AjnI CCWGG 3 cut(s) 44, 138, 330
AluBI AGCT 1 cut(s) 636
AluI AGCT 1 cut(s) 636
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 3 cut(s) 495, 617, 638
AlwI GGATC 2 cut(s) 194, 554
AoxI GGCC 4 cut(s) 110, 155, 165, 586
ApoI RAATTY 4 cut(s) 272, 407, 434, 483
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
AspLEI GCGC 1 cut(s) 431
AspS9I GGNCC 4 cut(s) 155, 166, 302, 586
AsuC2I CCSGG 2 cut(s) 164, 360
AsuII TTCGAA 1 cut(s) 70
AvaII GGWCC 1 cut(s) 302
BaeI ACNNNNGTAYC 2 cut(s) 378, 411
BauI CACGAG 1 cut(s) 212
BbsI GAAGAC 1 cut(s) 98
Bbv12I GWGCWC 1 cut(s) 126
BccI CCATC 2 cut(s) 146, 305
BcgI CGANNNNNNTGC 1 cut(s) 33
BciT130I CCWGG 3 cut(s) 46, 140, 332
BclI TGATCA 2 cut(s) 84, 291
BcnI CCSGG 2 cut(s) 164, 360
BcoDI GTCTC 3 cut(s) 495, 617, 638
BfaI CTAG 1 cut(s) 545
BisI GCNGC 3 cut(s) 21, 24, 351
BlsI GCNGC 3 cut(s) 22, 25, 352
Bme1390I CCNGG 5 cut(s) 46, 140, 164, 332, 360
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 4 cut(s) 155, 166, 302, 586
BmiI GGNNCC 2 cut(s) 221, 304
BmrFI CCNGG 5 cut(s) 46, 140, 164, 332, 360
BmrI ACTGGG 1 cut(s) 110
BmuI ACTGGG 1 cut(s) 110
BoxI GACNNNNGTC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 98
Bpu14I TTCGAA 1 cut(s) 70
BpuMI CCSGG 2 cut(s) 164, 360
BsaI GGTCTC 1 cut(s) 617
BsaJI CCNNGG 7 cut(s) 21, 44, 169, 330, 331, 450, 582
Bsc4I CCNNNNNNNGG 3 cut(s) 170, 311, 312
Bse1I ACTGG 1 cut(s) 105
BseBI CCWGG 3 cut(s) 46, 140, 332
BseDI CCNNGG 7 cut(s) 21, 44, 169, 330, 331, 450, 582
BseGI GGATG 1 cut(s) 577
BseLI CCNNNNNNNGG 3 cut(s) 170, 311, 312
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 105
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 4 cut(s) 112, 157, 167, 588
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 3 cut(s) 113, 164, 359
BslI CCNNNNNNNGG 3 cut(s) 170, 311, 312
BsmAI GTCTC 3 cut(s) 495, 617, 638
BsmBI CGTCTC 1 cut(s) 495
BsnI GGCC 4 cut(s) 112, 157, 167, 588
Bso31I GGTCTC 1 cut(s) 617
Bsp119I TTCGAA 1 cut(s) 70
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 4 cut(s) 84, 199, 291, 559
BspACI CCGC 4 cut(s) 21, 23, 78, 350
BspANI GGCC 4 cut(s) 112, 157, 167, 588
BspCNI CTCAG 1 cut(s) 235
BspFNI CGCG 1 cut(s) 23
BspHI TCATGA 1 cut(s) 87
BspLI GGNNCC 2 cut(s) 221, 304
BspPI GGATC 2 cut(s) 194, 554
BspQI GCTCTTC 1 cut(s) 669
BspT104I TTCGAA 1 cut(s) 70
BspTNI GGTCTC 1 cut(s) 617
BsrI ACTGG 1 cut(s) 105
BssECI CCNNGG 7 cut(s) 21, 44, 169, 330, 331, 450, 582
BssMI GATC 4 cut(s) 84, 199, 291, 559
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 2 cut(s) 169, 582
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 3 cut(s) 46, 140, 332
Bst4CI ACNGT 5 cut(s) 345, 451, 554, 568, 684
Bst6I CTCTTC 2 cut(s) 498, 669
BstBI TTCGAA 1 cut(s) 70
BstDEI CTNAG 1 cut(s) 243
BstDSI CCRYGG 2 cut(s) 21, 450
BstF5I GGATG 1 cut(s) 577
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 1 cut(s) 431
BstKTI GATC 4 cut(s) 87, 202, 294, 562
BstMAI GTCTC 3 cut(s) 495, 617, 638
BstMBI GATC 4 cut(s) 84, 199, 291, 559
BstMWI GCNNNNNNNGC 2 cut(s) 452, 461
BstNI CCWGG 3 cut(s) 46, 140, 332
BstPAI GACNNNNGTC 1 cut(s) 264
BstSCI CCNGG 5 cut(s) 44, 138, 162, 330, 358
BstUI CGCG 1 cut(s) 23
BstV2I GAAGAC 1 cut(s) 98
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
BsuRI GGCC 4 cut(s) 112, 157, 167, 588
BtgI CCRYGG 2 cut(s) 21, 450
BtsCI GGATG 1 cut(s) 577
BtsI GCAGTG 1 cut(s) 353
BtsIMutI CAGTG 2 cut(s) 353, 383
CciI TCATGA 1 cut(s) 87
CfoI GCGC 1 cut(s) 431
Cfr13I GGNCC 4 cut(s) 155, 166, 302, 586
Cfr42I CCGCGG 1 cut(s) 24
Csp6I GTAC 2 cut(s) 247, 395
CviAII CATG 3 cut(s) 62, 88, 289
CviQI GTAC 2 cut(s) 247, 395
DdeI CTNAG 1 cut(s) 243
DpnI GATC 4 cut(s) 86, 201, 293, 561
DpnII GATC 4 cut(s) 84, 199, 291, 559
EaeI YGGCCR 1 cut(s) 110
Eam1104I CTCTTC 2 cut(s) 498, 669
EarI CTCTTC 2 cut(s) 498, 669
Eco130I CCWWGG 2 cut(s) 169, 582
Eco31I GGTCTC 1 cut(s) 617
Eco47I GGWCC 1 cut(s) 302
EcoRI GAATTC 1 cut(s) 272
EcoRII CCWGG 3 cut(s) 44, 138, 330
EcoT14I CCWWGG 2 cut(s) 169, 582
ErhI CCWWGG 2 cut(s) 169, 582
Esp3I CGTCTC 1 cut(s) 495
FaeI CATG 3 cut(s) 65, 91, 292
FaiI YATR 6 cut(s) 63, 89, 290, 529, 531, 632
FatI CATG 3 cut(s) 61, 87, 288
FauNDI CATATG 1 cut(s) 529
FbaI TGATCA 2 cut(s) 84, 291
FblI GTMKAC 1 cut(s) 364
Fnu4HI GCNGC 3 cut(s) 21, 24, 351
FokI GGATG 1 cut(s) 564
Fsp4HI GCNGC 3 cut(s) 21, 24, 351
FspBI CTAG 1 cut(s) 545
GlaI GCGC 1 cut(s) 430
GluI GCNGC 3 cut(s) 21, 24, 351
HaeIII GGCC 4 cut(s) 112, 157, 167, 588
HapII CCGG 3 cut(s) 113, 164, 359
HhaI GCGC 1 cut(s) 431
Hin1II CATG 3 cut(s) 65, 91, 292
Hin6I GCGC 1 cut(s) 429
HinP1I GCGC 1 cut(s) 429
HinfI GANTC 5 cut(s) 32, 116, 534, 571, 603
HpaII CCGG 3 cut(s) 113, 164, 359
Hpy166II GTNNAC 2 cut(s) 249, 365
Hpy188I TCNGA 4 cut(s) 31, 181, 244, 654
Hpy188III TCNNGA 6 cut(s) 88, 149, 212, 269, 295, 520
Hpy8I GTNNAC 2 cut(s) 249, 365
HpyAV CCTTC 2 cut(s) 68, 664
HpyCH4III ACNGT 5 cut(s) 345, 451, 554, 568, 684
HpyF10VI GCNNNNNNNGC 2 cut(s) 452, 461
HpyF3I CTNAG 1 cut(s) 243
Hsp92II CATG 3 cut(s) 65, 91, 292
HspAI GCGC 1 cut(s) 429
Ksp22I TGATCA 2 cut(s) 84, 291
KspI CCGCGG 1 cut(s) 24
Kzo9I GATC 4 cut(s) 84, 199, 291, 559
LguI GCTCTTC 1 cut(s) 669
MaeI CTAG 1 cut(s) 545
MalI GATC 4 cut(s) 86, 201, 293, 561
MboI GATC 4 cut(s) 84, 199, 291, 559
MboII GAAGA 5 cut(s) 103, 293, 485, 667, 686
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 9 cut(s) 130, 224, 272, 402, 407, 420, 434, 483, 648
MlyI GAGTC 3 cut(s) 125, 528, 597
MmeI TCCRAC 1 cut(s) 642
MnlI CCTC 6 cut(s) 4, 30, 181, 213, 419, 422
MseI TTAA 1 cut(s) 405
MspA1I CMGCKG 3 cut(s) 23, 78, 350
MspI CCGG 3 cut(s) 113, 164, 359
MspR9I CCNGG 5 cut(s) 46, 140, 164, 332, 360
MvaI CCWGG 3 cut(s) 46, 140, 332
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 2 cut(s) 452, 461
NciI CCSGG 2 cut(s) 164, 360
NdeI CATATG 1 cut(s) 529
NdeII GATC 4 cut(s) 84, 199, 291, 559
NlaIII CATG 3 cut(s) 65, 91, 292
NlaIV GGNNCC 2 cut(s) 221, 304
NspV TTCGAA 1 cut(s) 70
PagI TCATGA 1 cut(s) 87
PasI CCCWGGG 1 cut(s) 331
PciSI GCTCTTC 1 cut(s) 669
PfeI GAWTC 2 cut(s) 32, 571
PkrI GCNGC 3 cut(s) 22, 25, 352
PleI GAGTC 3 cut(s) 124, 528, 597
PpsI GAGTC 3 cut(s) 124, 528, 597
PshAI GACNNNNGTC 1 cut(s) 264
Psp6I CCWGG 3 cut(s) 44, 138, 330
PspGI CCWGG 3 cut(s) 44, 138, 330
PspN4I GGNNCC 2 cut(s) 221, 304
PspPI GGNCC 4 cut(s) 155, 166, 302, 586
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 2 cut(s) 248, 396
RsaNI GTAC 2 cut(s) 247, 395
SacII CCGCGG 1 cut(s) 24
SapI GCTCTTC 1 cut(s) 669
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 3 cut(s) 21, 24, 351
Sau3AI GATC 4 cut(s) 84, 199, 291, 559
Sau96I GGNCC 4 cut(s) 155, 166, 302, 586
SchI GAGTC 3 cut(s) 125, 528, 597
ScrFI CCNGG 5 cut(s) 46, 140, 164, 332, 360
SduI GDGCHC 1 cut(s) 126
SetI ASST 5 cut(s) 50, 141, 175, 400, 638
Sfr303I CCGCGG 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 70
SgrBI CCGCGG 1 cut(s) 24
SinI GGWCC 1 cut(s) 302
Sse9I AATT 9 cut(s) 130, 224, 272, 402, 407, 420, 434, 483, 648
SsiI CCGC 4 cut(s) 21, 23, 78, 350
SspMI CTAG 1 cut(s) 545
StyD4I CCNGG 5 cut(s) 44, 138, 162, 330, 358
StyI CCWWGG 2 cut(s) 169, 582
TaaI ACNGT 5 cut(s) 345, 451, 554, 568, 684
TaqI TCGA 8 cut(s) 35, 70, 135, 150, 186, 198, 537, 606
TasI AATT 9 cut(s) 130, 224, 272, 402, 407, 420, 434, 483, 648
TatI WGTACW 1 cut(s) 246
TauI GCSGC 3 cut(s) 23, 26, 353
TfiI GAWTC 2 cut(s) 32, 571
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 2 cut(s) 360, 390
TspDTI ATGAA 7 cut(s) 50, 104, 265, 459, 563, 624, 628
TspGWI ACGGA 1 cut(s) 477
TspRI CASTG 2 cut(s) 360, 390
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 4 cut(s) 272, 407, 434, 483
XmiI GTMKAC 1 cut(s) 364
XspI CTAG 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.