RchiOBHm_Chr7g0237691

Dihydrofolate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008449
Physical Location & Seq
Reverse (-)
62856672 .. 62858994
2323 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21302

Sequence Viewer

Length: 1161 bp
ATGTTTTCAGATGGCCCCCCCAATCGAAACAAGTTTGCCTTTTTTTATTCTTTCATATTTCAAATAGGGGCTATCAAATTACCAAACGCCCCTTCCTTTTGTACCAATAGATTTCTGTCTCCGAGGGTGTTATTTCCCACGCGGGTCAAAACCTGCGTTCTCTCCCTCTCTTTCGTCCCTCGTTCGCCACTTCGCCTTGCCCTGATGTCATCCAACGCATCATCCACCCTCCACTTAAACCGCACGCCCATTCTGGACCGTCGATTTCCGCTACGCGGTCTACGCAACCTATTTAGTGTTCCCCATTCCCTTCACTCCATTCGCATTGAAAGCAGGAAACTCCAACACGACGTCGTTGCGACCAAGGCCGCGCCTTTTAAATCCCCCACCACCTCCCGCACTAGCGGAACTGACCAGAACAACAACAACAGCCTCAACCTTTCCGAAACGACAAGGAACACCAACAACAACAACATGGGAAGCGAGGGTTGTGCCGCGGCTTCTGATTCGAGGAAACCCAGGTTTCTTTGCCTTCATGGGTTTCGAACCAGCGGTGTGATCATGAAGACCCAACTGGGTAAATGGCCGGAGTCGGTGCTCAACAATTTCGACCTGGACTTTCTCGATGGGCCTTTCCCGGCCCAAGGTAAATCCGAAGTCGAGGGCATTTTCGATCCTCCTTATCACGAGTGGTTCCAATTCAGCAAGGATTTTCTTGAGTACACGAACTTTGACGAGTGTCTTGAATTCATTGAAGATTACATGATCAAGAATGGACCCTTTGATGGGCTACTTGGCTTTTCCCAGGGGGCAATACTGTCAGCGGCACTGCCGGGTCTACAAGCAAAGGGTTTGGCACTGACCAAAGTACCTAAAATTAAGTTCCTCATAATAATTGGAGGCGCAAAATTCAAGTCAGCAACCGTGGCTGAAAATGCTTATTCATCTCCTATTCAAATTCCGTCTCTTCACTTTTTAGGAGAAACAGACTTCTTGAAGCCATATGGACTCGAACTGCTAGAAAACTGTGTAGATCCTACCGTGATTCATCATCCCAAGGGCCACACAATACCACGACTCGATGAAAAGGGTTTAGAGACCATTATGAGCTTCATTGAGACAATTCAGAAGAAGTTGGACGAGAAGGAGGAGCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1

Protein Analysis

386

Amino Acids

43.19

Weight (kDa)

8.79

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 171 - 362 2.8e-52 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 161
AccI GTMKAC 2 cut(s) 280, 838
AccII CGCG 4 cut(s) 142, 276, 371, 497
AclWI GGATC 2 cut(s) 668, 1028
AcoI YGGCCR 1 cut(s) 584
AcsI RAATTY 3 cut(s) 746, 908, 957
AcyI GRCGYC 1 cut(s) 351
AfaI GTAC 3 cut(s) 103, 722, 870
AfiI CCNNNNNNNGG 4 cut(s) 275, 644, 785, 786
AgsI TTSAA 7 cut(s) 62, 329, 746, 755, 913, 956, 997
AjnI CCWGG 3 cut(s) 518, 612, 804
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
AluBI AGCT 1 cut(s) 1110
AluI AGCT 1 cut(s) 1110
Alw21I GWGCWC 1 cut(s) 600
Alw26I GTCTC 4 cut(s) 123, 969, 1091, 1112
AlwI GGATC 2 cut(s) 668, 1028
AoxI GGCC 6 cut(s) 13, 366, 584, 629, 639, 1060
ApoI RAATTY 3 cut(s) 746, 908, 957
ArsI GACNNNNNNTTYG 2 cut(s) 899, 931
AspLEI GCGC 2 cut(s) 373, 905
AspS9I GGNCC 6 cut(s) 14, 256, 629, 640, 776, 1060
AsuC2I CCSGG 2 cut(s) 638, 834
AsuII TTCGAA 1 cut(s) 544
AvaII GGWCC 2 cut(s) 256, 776
BaeI ACNNNNGTAYC 2 cut(s) 852, 885
BauI CACGAG 1 cut(s) 686
BbsI GAAGAC 1 cut(s) 572
Bbv12I GWGCWC 1 cut(s) 600
BccI CCATC 3 cut(s) 5, 620, 779
BcgI CGANNNNNNTGC 4 cut(s) 338, 372, 473, 507
BciT130I CCWGG 3 cut(s) 520, 614, 806
BclI TGATCA 2 cut(s) 558, 765
BcnI CCSGG 2 cut(s) 638, 834
BcoDI GTCTC 4 cut(s) 123, 969, 1091, 1112
BfaI CTAG 2 cut(s) 402, 1019
BfuAI ACCTGC 1 cut(s) 161
BisI GCNGC 4 cut(s) 369, 495, 498, 825
BlsI GCNGC 4 cut(s) 370, 496, 499, 826
Bme1390I CCNGG 5 cut(s) 520, 614, 638, 806, 834
Bme18I GGWCC 2 cut(s) 256, 776
BmgT120I GGNCC 6 cut(s) 14, 256, 629, 640, 776, 1060
BmiI GGNNCC 3 cut(s) 16, 695, 778
BmrFI CCNGG 5 cut(s) 520, 614, 638, 806, 834
BmrI ACTGGG 1 cut(s) 584
BmsI GCATC 1 cut(s) 227
BmuI ACTGGG 1 cut(s) 584
BoxI GACNNNNGTC 1 cut(s) 738
BpiI GAAGAC 1 cut(s) 572
Bpu14I TTCGAA 1 cut(s) 544
BpuEI CTTGAG 1 cut(s) 737
BpuMI CCSGG 2 cut(s) 638, 834
BsaHI GRCGYC 1 cut(s) 351
BsaI GGTCTC 1 cut(s) 1091
BsaJI CCNNGG 9 cut(s) 122, 363, 495, 518, 643, 804, 805, 924, 1056
BsaXI ACNNNNNCTCC 1 cut(s) 1142
Bsc4I CCNNNNNNNGG 4 cut(s) 275, 644, 785, 786
Bse1I ACTGG 1 cut(s) 579
BseBI CCWGG 3 cut(s) 520, 614, 806
BseDI CCNNGG 9 cut(s) 122, 363, 495, 518, 643, 804, 805, 924, 1056
BseGI GGATG 3 cut(s) 209, 221, 1051
BseLI CCNNNNNNNGG 4 cut(s) 275, 644, 785, 786
BseNI ACTGG 1 cut(s) 579
Bsh1236I CGCG 4 cut(s) 142, 276, 371, 497
BshFI GGCC 6 cut(s) 15, 368, 586, 631, 641, 1062
BsiHKAI GWGCWC 1 cut(s) 600
BsiSI CCGG 3 cut(s) 587, 638, 833
BslFI GGGAC 1 cut(s) 161
BslI CCNNNNNNNGG 4 cut(s) 275, 644, 785, 786
BsmAI GTCTC 4 cut(s) 123, 969, 1091, 1112
BsmBI CGTCTC 1 cut(s) 969
BsmFI GGGAC 1 cut(s) 161
BsnI GGCC 6 cut(s) 15, 368, 586, 631, 641, 1062
Bso31I GGTCTC 1 cut(s) 1091
Bsp119I TTCGAA 1 cut(s) 544
Bsp1286I GDGCHC 1 cut(s) 600
Bsp143I GATC 4 cut(s) 558, 673, 765, 1033
BspANI GGCC 6 cut(s) 15, 368, 586, 631, 641, 1062
BspFNI CGCG 4 cut(s) 142, 276, 371, 497
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 3 cut(s) 16, 695, 778
BspMI ACCTGC 1 cut(s) 161
BspPI GGATC 2 cut(s) 668, 1028
BspT104I TTCGAA 1 cut(s) 544
BspTNI GGTCTC 1 cut(s) 1091
BsrI ACTGG 1 cut(s) 579
BssECI CCNNGG 9 cut(s) 122, 363, 495, 518, 643, 804, 805, 924, 1056
BssMI GATC 4 cut(s) 558, 673, 765, 1033
BssNI GRCGYC 1 cut(s) 351
BssSI CACGAG 1 cut(s) 686
BssT1I CCWWGG 3 cut(s) 363, 643, 1056
Bst2BI CACGAG 1 cut(s) 686
Bst2UI CCWGG 3 cut(s) 520, 614, 806
Bst4CI ACNGT 6 cut(s) 260, 819, 925, 1028, 1042, 1158
Bst6I CTCTTC 1 cut(s) 972
BstACI GRCGYC 1 cut(s) 351
BstBI TTCGAA 1 cut(s) 544
BstC8I GCNNGC 1 cut(s) 245
BstDSI CCRYGG 2 cut(s) 495, 924
BstF5I GGATG 3 cut(s) 209, 221, 1051
BstFNI CGCG 4 cut(s) 142, 276, 371, 497
BstHHI GCGC 2 cut(s) 373, 905
BstKTI GATC 4 cut(s) 561, 676, 768, 1036
BstMAI GTCTC 4 cut(s) 123, 969, 1091, 1112
BstMBI GATC 4 cut(s) 558, 673, 765, 1033
BstMWI GCNNNNNNNGC 5 cut(s) 282, 330, 365, 926, 935
BstNI CCWGG 3 cut(s) 520, 614, 806
BstPAI GACNNNNGTC 1 cut(s) 738
BstSCI CCNGG 5 cut(s) 518, 612, 636, 804, 832
BstUI CGCG 4 cut(s) 142, 276, 371, 497
BstV2I GAAGAC 1 cut(s) 572
BstX2I RGATCY 1 cut(s) 1033
BstYI RGATCY 1 cut(s) 1033
BsuRI GGCC 6 cut(s) 15, 368, 586, 631, 641, 1062
BtgI CCRYGG 2 cut(s) 495, 924
BtsCI GGATG 3 cut(s) 209, 221, 1051
BtsI GCAGTG 1 cut(s) 827
BtsIMutI CAGTG 2 cut(s) 827, 857
BveI ACCTGC 1 cut(s) 161
Cac8I GCNNGC 1 cut(s) 245
CciI TCATGA 1 cut(s) 561
CfoI GCGC 2 cut(s) 373, 905
Cfr13I GGNCC 6 cut(s) 14, 256, 629, 640, 776, 1060
Cfr42I CCGCGG 1 cut(s) 498
Csp6I GTAC 3 cut(s) 102, 721, 869
CviAII CATG 4 cut(s) 475, 536, 562, 763
CviQI GTAC 3 cut(s) 102, 721, 869
DpnI GATC 4 cut(s) 560, 675, 767, 1035
DpnII GATC 4 cut(s) 558, 673, 765, 1033
DraI TTTAAA 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 584
Eam1104I CTCTTC 1 cut(s) 972
EarI CTCTTC 1 cut(s) 972
Eco130I CCWWGG 3 cut(s) 363, 643, 1056
Eco31I GGTCTC 1 cut(s) 1091
Eco47I GGWCC 2 cut(s) 256, 776
EcoRI GAATTC 1 cut(s) 746
EcoRII CCWGG 3 cut(s) 518, 612, 804
EcoT14I CCWWGG 3 cut(s) 363, 643, 1056
ErhI CCWWGG 3 cut(s) 363, 643, 1056
Esp3I CGTCTC 1 cut(s) 969
FaeI CATG 4 cut(s) 478, 539, 565, 766
FaiI YATR 9 cut(s) 56, 476, 537, 563, 764, 890, 1003, 1005, 1106
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FaqI GGGAC 1 cut(s) 161
FatI CATG 4 cut(s) 474, 535, 561, 762
FauI CCCGC 2 cut(s) 135, 404
FauNDI CATATG 1 cut(s) 1003
FbaI TGATCA 2 cut(s) 558, 765
FblI GTMKAC 2 cut(s) 280, 838
Fnu4HI GCNGC 4 cut(s) 369, 495, 498, 825
FokI GGATG 3 cut(s) 196, 208, 1038
Fsp4HI GCNGC 4 cut(s) 369, 495, 498, 825
FspBI CTAG 2 cut(s) 402, 1019
GlaI GCGC 2 cut(s) 372, 904
GluI GCNGC 4 cut(s) 369, 495, 498, 825
HaeIII GGCC 6 cut(s) 15, 368, 586, 631, 641, 1062
HapII CCGG 3 cut(s) 587, 638, 833
HhaI GCGC 2 cut(s) 373, 905
Hin1I GRCGYC 1 cut(s) 351
Hin1II CATG 4 cut(s) 478, 539, 565, 766
Hin6I GCGC 2 cut(s) 371, 903
HinP1I GCGC 2 cut(s) 371, 903
HinfI GANTC 5 cut(s) 506, 590, 1008, 1045, 1077
HpaII CCGG 3 cut(s) 587, 638, 833
Hpy166II GTNNAC 3 cut(s) 281, 723, 839
Hpy188I TCNGA 6 cut(s) 10, 123, 445, 505, 655, 1128
Hpy188III TCNNGA 8 cut(s) 254, 562, 623, 686, 716, 743, 769, 994
Hpy8I GTNNAC 3 cut(s) 281, 723, 839
Hpy99I CGWCG 3 cut(s) 264, 353, 356
HpyAV CCTTC 4 cut(s) 102, 320, 542, 1138
HpyCH4III ACNGT 6 cut(s) 260, 819, 925, 1028, 1042, 1158
HpyCH4IV ACGT 1 cut(s) 351
HpyF10VI GCNNNNNNNGC 5 cut(s) 282, 330, 365, 926, 935
HpySE526I ACGT 1 cut(s) 351
Hsp92I GRCGYC 1 cut(s) 351
Hsp92II CATG 4 cut(s) 478, 539, 565, 766
HspAI GCGC 2 cut(s) 371, 903
Ksp22I TGATCA 2 cut(s) 558, 765
KspI CCGCGG 1 cut(s) 498
Kzo9I GATC 4 cut(s) 558, 673, 765, 1033
LmnI GCTCC 1 cut(s) 1150
LweI GCATC 1 cut(s) 227
MaeI CTAG 2 cut(s) 402, 1019
MaeII ACGT 1 cut(s) 351
MalI GATC 4 cut(s) 560, 675, 767, 1035
MboI GATC 4 cut(s) 558, 673, 765, 1033
MboII GAAGA 4 cut(s) 577, 767, 959, 1141
MflI RGATCY 1 cut(s) 1033
MhlI GDGCHC 1 cut(s) 600
MluCI AATT 9 cut(s) 77, 604, 698, 746, 876, 894, 908, 957, 1122
MlyI GAGTC 3 cut(s) 599, 1002, 1071
MmeI TCCRAC 3 cut(s) 237, 367, 1116
MseI TTAA 3 cut(s) 236, 378, 879
MspA1I CMGCKG 3 cut(s) 497, 552, 824
MspI CCGG 3 cut(s) 587, 638, 833
MspR9I CCNGG 5 cut(s) 520, 614, 638, 806, 834
MvaI CCWGG 3 cut(s) 520, 614, 806
MvnI CGCG 4 cut(s) 142, 276, 371, 497
MwoI GCNNNNNNNGC 5 cut(s) 282, 330, 365, 926, 935
NciI CCSGG 2 cut(s) 638, 834
NdeI CATATG 1 cut(s) 1003
NdeII GATC 4 cut(s) 558, 673, 765, 1033
NlaIII CATG 4 cut(s) 478, 539, 565, 766
NlaIV GGNNCC 3 cut(s) 16, 695, 778
NspV TTCGAA 1 cut(s) 544
PagI TCATGA 1 cut(s) 561
PasI CCCWGGG 1 cut(s) 805
PfeI GAWTC 2 cut(s) 506, 1045
PkrI GCNGC 4 cut(s) 370, 496, 499, 826
PleI GAGTC 3 cut(s) 598, 1002, 1071
PpsI GAGTC 3 cut(s) 598, 1002, 1071
PshAI GACNNNNGTC 1 cut(s) 738
Psp6I CCWGG 3 cut(s) 518, 612, 804
PspGI CCWGG 3 cut(s) 518, 612, 804
PspN4I GGNNCC 3 cut(s) 16, 695, 778
PspPI GGNCC 6 cut(s) 14, 256, 629, 640, 776, 1060
PsuI RGATCY 1 cut(s) 1033
RsaI GTAC 3 cut(s) 103, 722, 870
RsaNI GTAC 3 cut(s) 102, 721, 869
SacII CCGCGG 1 cut(s) 498
SaqAI TTAA 3 cut(s) 236, 378, 879
SatI GCNGC 4 cut(s) 369, 495, 498, 825
Sau3AI GATC 4 cut(s) 558, 673, 765, 1033
Sau96I GGNCC 6 cut(s) 14, 256, 629, 640, 776, 1060
SchI GAGTC 3 cut(s) 599, 1002, 1071
ScrFI CCNGG 5 cut(s) 520, 614, 638, 806, 834
SduI GDGCHC 1 cut(s) 600
SfaNI GCATC 1 cut(s) 227
Sfr303I CCGCGG 1 cut(s) 498
SfuI TTCGAA 1 cut(s) 544
SgrBI CCGCGG 1 cut(s) 498
SinI GGWCC 2 cut(s) 256, 776
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
Sse9I AATT 9 cut(s) 77, 604, 698, 746, 876, 894, 908, 957, 1122
SspMI CTAG 2 cut(s) 402, 1019
StyD4I CCNGG 5 cut(s) 518, 612, 636, 804, 832
StyI CCWWGG 3 cut(s) 363, 643, 1056
TaaI ACNGT 6 cut(s) 260, 819, 925, 1028, 1042, 1158
TaiI ACGT 1 cut(s) 354
TasI AATT 9 cut(s) 77, 604, 698, 746, 876, 894, 908, 957, 1122
TatI WGTACW 1 cut(s) 720
TauI GCSGC 4 cut(s) 371, 497, 500, 827
TfiI GAWTC 2 cut(s) 506, 1045
Tru1I TTAA 3 cut(s) 236, 378, 879
Tru9I TTAA 3 cut(s) 236, 378, 879
TscAI CASTG 2 cut(s) 834, 864
TspDTI ATGAA 8 cut(s) 43, 524, 578, 739, 933, 1037, 1098, 1102
TspGWI ACGGA 1 cut(s) 951
TspRI CASTG 2 cut(s) 834, 864
VpaK11BI GGWCC 2 cut(s) 256, 776
XapI RAATTY 3 cut(s) 746, 908, 957
XmiI GTMKAC 2 cut(s) 280, 838
XspI CTAG 2 cut(s) 402, 1019
ZraI GACGTC 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.