Rh7BG433200

Dihydrofolate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0008449
Physical Location & Seq
Reverse (-)
49683861 .. 49685370
1510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG433200.1

Sequence Viewer

Length: 600 bp
ATGAAGACCCAACTGGGTAAATGGCCGGAGTCGGTGCTCAAGAATTTCGACCTGGACTTTCTCGATGGGCCTTTCCCGGCCCAAGGTAAATCCGAAGTCGAGGGCATTTTCGATCCTCCTTATCACGAGTGGTTCCAATTCAGCAAGGATTTTTCTGAGTACACGAACTTTGACGAGTGTCTTGAATTCATTGAAGATTACATGATCAAGAATGGACCCTTTGATGGGCTACTTGGCTTTTCCCAGGGAGCAATACTGTCAGCGGCACTGCCGGGTCTACAAGCAAAGGGTTTGGCATTGACCAAAGTACCTAAAATTAAATTCCTCGTAATAATTGGAGGCGCAAAATTCAAGTCAGCAACCTTGGCTGAAAATGCTTATTCATCTCCTATTCAAATTCCGTCTCTTCACTTTTTAGGAGAAACAGACTTCTTGAAGCCATATGGACTCGAACTGCTAAAAAACTGTGTAGATCCTACCGTGATTCATCATCCCAAGGGCCACACGATACCACGACTCGATGAAAAGGGTTTAGAGACCATTATGAGCTTCATTGAGACAATTCAGAAGAAATTGGACGAGAAGGAGGAGCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1

Protein Analysis

199

Amino Acids

22.37

Weight (kDa)

5.05

Isoelectric Point (pI)

31.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 3 - 175 3.4e-45 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 277
AciI CCGC 1 cut(s) 263
AclWI GGATC 2 cut(s) 107, 467
AcoI YGGCCR 1 cut(s) 23
AcsI RAATTY 5 cut(s) 43, 185, 320, 347, 396
AfaI GTAC 2 cut(s) 161, 309
AfiI CCNNNNNNNGG 3 cut(s) 83, 224, 225
AgsI TTSAA 5 cut(s) 185, 194, 352, 395, 436
AjnI CCWGG 2 cut(s) 51, 243
AluBI AGCT 1 cut(s) 549
AluI AGCT 1 cut(s) 549
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 3 cut(s) 408, 530, 551
AlwI GGATC 2 cut(s) 107, 467
AoxI GGCC 4 cut(s) 23, 68, 78, 499
ApoI RAATTY 5 cut(s) 43, 185, 320, 347, 396
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 4 cut(s) 68, 79, 215, 499
AsuC2I CCSGG 2 cut(s) 77, 273
AvaII GGWCC 1 cut(s) 215
BaeI ACNNNNGTAYC 2 cut(s) 291, 324
BauI CACGAG 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 39
BccI CCATC 2 cut(s) 59, 218
BciT130I CCWGG 2 cut(s) 53, 245
BclI TGATCA 1 cut(s) 204
BcnI CCSGG 2 cut(s) 77, 273
BcoDI GTCTC 3 cut(s) 408, 530, 551
BisI GCNGC 1 cut(s) 264
BlsI GCNGC 1 cut(s) 265
Bme1390I CCNGG 4 cut(s) 53, 77, 245, 273
Bme18I GGWCC 1 cut(s) 215
BmgT120I GGNCC 4 cut(s) 68, 79, 215, 499
BmiI GGNNCC 2 cut(s) 134, 217
BmrFI CCNGG 4 cut(s) 53, 77, 245, 273
BmrI ACTGGG 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 23
BoxI GACNNNNGTC 1 cut(s) 177
BpiI GAAGAC 1 cut(s) 11
BpuEI CTTGAG 1 cut(s) 23
BpuMI CCSGG 2 cut(s) 77, 273
BsaI GGTCTC 1 cut(s) 530
BsaJI CCNNGG 5 cut(s) 82, 243, 244, 363, 495
BsaXI ACNNNNNCTCC 1 cut(s) 581
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 224, 225
Bse1I ACTGG 1 cut(s) 18
BseBI CCWGG 2 cut(s) 53, 245
BseDI CCNNGG 5 cut(s) 82, 243, 244, 363, 495
BseGI GGATG 1 cut(s) 490
BseLI CCNNNNNNNGG 3 cut(s) 83, 224, 225
BseMII CTCAG 1 cut(s) 147
BseNI ACTGG 1 cut(s) 18
BshFI GGCC 4 cut(s) 25, 70, 80, 501
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 3 cut(s) 26, 77, 272
BslI CCNNNNNNNGG 3 cut(s) 83, 224, 225
BsmAI GTCTC 3 cut(s) 408, 530, 551
BsmBI CGTCTC 1 cut(s) 408
BsnI GGCC 4 cut(s) 25, 70, 80, 501
Bso31I GGTCTC 1 cut(s) 530
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 3 cut(s) 112, 204, 472
BspACI CCGC 1 cut(s) 263
BspANI GGCC 4 cut(s) 25, 70, 80, 501
BspCNI CTCAG 1 cut(s) 148
BspLI GGNNCC 2 cut(s) 134, 217
BspPI GGATC 2 cut(s) 107, 467
BspTNI GGTCTC 1 cut(s) 530
BsrI ACTGG 1 cut(s) 18
BssECI CCNNGG 5 cut(s) 82, 243, 244, 363, 495
BssMI GATC 3 cut(s) 112, 204, 472
BssSI CACGAG 1 cut(s) 125
BssT1I CCWWGG 3 cut(s) 82, 363, 495
Bst2BI CACGAG 1 cut(s) 125
Bst2UI CCWGG 2 cut(s) 53, 245
Bst4CI ACNGT 4 cut(s) 258, 467, 481, 597
Bst6I CTCTTC 1 cut(s) 411
BstDEI CTNAG 1 cut(s) 156
BstF5I GGATG 1 cut(s) 490
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 3 cut(s) 115, 207, 475
BstMAI GTCTC 3 cut(s) 408, 530, 551
BstMBI GATC 3 cut(s) 112, 204, 472
BstMWI GCNNNNNNNGC 2 cut(s) 365, 374
BstNI CCWGG 2 cut(s) 53, 245
BstPAI GACNNNNGTC 1 cut(s) 177
BstSCI CCNGG 4 cut(s) 51, 75, 243, 271
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
BsuRI GGCC 4 cut(s) 25, 70, 80, 501
BtsCI GGATG 1 cut(s) 490
BtsI GCAGTG 1 cut(s) 266
BtsIMutI CAGTG 1 cut(s) 266
CfoI GCGC 1 cut(s) 344
Cfr13I GGNCC 4 cut(s) 68, 79, 215, 499
Csp6I GTAC 2 cut(s) 160, 308
CviAII CATG 1 cut(s) 202
CviJI RGCY 9 cut(s) 25, 70, 80, 229, 237, 368, 439, 501, 549
CviKI_1 RGCY 9 cut(s) 25, 70, 80, 229, 237, 368, 439, 501, 549
CviQI GTAC 2 cut(s) 160, 308
DdeI CTNAG 1 cut(s) 156
DpnI GATC 3 cut(s) 114, 206, 474
DpnII GATC 3 cut(s) 112, 204, 472
EaeI YGGCCR 1 cut(s) 23
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
Eco130I CCWWGG 3 cut(s) 82, 363, 495
Eco31I GGTCTC 1 cut(s) 530
Eco47I GGWCC 1 cut(s) 215
EcoRI GAATTC 1 cut(s) 185
EcoRII CCWGG 2 cut(s) 51, 243
EcoT14I CCWWGG 3 cut(s) 82, 363, 495
ErhI CCWWGG 3 cut(s) 82, 363, 495
Esp3I CGTCTC 1 cut(s) 408
FaeI CATG 1 cut(s) 205
FaiI YATR 4 cut(s) 203, 442, 444, 545
FatI CATG 1 cut(s) 201
FauNDI CATATG 1 cut(s) 442
FbaI TGATCA 1 cut(s) 204
FblI GTMKAC 1 cut(s) 277
Fnu4HI GCNGC 1 cut(s) 264
FokI GGATG 1 cut(s) 477
Fsp4HI GCNGC 1 cut(s) 264
GlaI GCGC 1 cut(s) 343
GluI GCNGC 1 cut(s) 264
HaeIII GGCC 4 cut(s) 25, 70, 80, 501
HapII CCGG 3 cut(s) 26, 77, 272
HhaI GCGC 1 cut(s) 344
Hin1II CATG 1 cut(s) 205
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HinfI GANTC 4 cut(s) 29, 447, 484, 516
HpaII CCGG 3 cut(s) 26, 77, 272
Hpy166II GTNNAC 2 cut(s) 162, 278
Hpy188I TCNGA 3 cut(s) 94, 157, 567
Hpy188III TCNNGA 6 cut(s) 40, 62, 125, 182, 208, 433
Hpy8I GTNNAC 2 cut(s) 162, 278
HpyAV CCTTC 1 cut(s) 577
HpyCH4III ACNGT 4 cut(s) 258, 467, 481, 597
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 374
HpyF3I CTNAG 1 cut(s) 156
Hsp92II CATG 1 cut(s) 205
HspAI GCGC 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 204
Kzo9I GATC 3 cut(s) 112, 204, 472
LmnI GCTCC 2 cut(s) 248, 589
LpnPI CCDG 7 cut(s) 38, 39, 65, 90, 230, 257, 285
MalI GATC 3 cut(s) 114, 206, 474
MboI GATC 3 cut(s) 112, 204, 472
MboII GAAGA 4 cut(s) 16, 206, 398, 580
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 1 cut(s) 39
MlyI GAGTC 3 cut(s) 38, 441, 510
MnlI CCTC 5 cut(s) 94, 126, 332, 335, 580
MseI TTAA 1 cut(s) 318
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 3 cut(s) 26, 77, 272
MspR9I CCNGG 4 cut(s) 53, 77, 245, 273
MvaI CCWGG 2 cut(s) 53, 245
MwoI GCNNNNNNNGC 2 cut(s) 365, 374
NciI CCSGG 2 cut(s) 77, 273
NdeI CATATG 1 cut(s) 442
NdeII GATC 3 cut(s) 112, 204, 472
NlaIII CATG 1 cut(s) 205
NlaIV GGNNCC 2 cut(s) 134, 217
PasI CCCWGGG 1 cut(s) 244
PfeI GAWTC 1 cut(s) 484
PkrI GCNGC 1 cut(s) 265
PleI GAGTC 3 cut(s) 37, 441, 510
PpsI GAGTC 3 cut(s) 37, 441, 510
PshAI GACNNNNGTC 1 cut(s) 177
Psp6I CCWGG 2 cut(s) 51, 243
PspGI CCWGG 2 cut(s) 51, 243
PspN4I GGNNCC 2 cut(s) 134, 217
PspPI GGNCC 4 cut(s) 68, 79, 215, 499
PsuI RGATCY 1 cut(s) 472
RsaI GTAC 2 cut(s) 161, 309
RsaNI GTAC 2 cut(s) 160, 308
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 1 cut(s) 264
Sau3AI GATC 3 cut(s) 112, 204, 472
Sau96I GGNCC 4 cut(s) 68, 79, 215, 499
SchI GAGTC 3 cut(s) 38, 441, 510
ScrFI CCNGG 4 cut(s) 53, 77, 245, 273
SduI GDGCHC 1 cut(s) 39
SetI ASST 5 cut(s) 54, 88, 313, 365, 551
SinI GGWCC 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
SsiI CCGC 1 cut(s) 263
StyD4I CCNGG 4 cut(s) 51, 75, 243, 271
StyI CCWWGG 3 cut(s) 82, 363, 495
TaaI ACNGT 4 cut(s) 258, 467, 481, 597
TaqI TCGA 6 cut(s) 48, 63, 99, 111, 450, 519
TatI WGTACW 1 cut(s) 159
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 1 cut(s) 484
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 1 cut(s) 273
TspDTI ATGAA 6 cut(s) 17, 178, 372, 476, 537, 541
TspGWI ACGGA 1 cut(s) 390
TspRI CASTG 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 5 cut(s) 43, 185, 320, 347, 396
XmiI GTMKAC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.