Rh7CG480000

Dihydrofolate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0008449
Physical Location & Seq
Reverse (-)
64473076 .. 64476404
3329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG480000.1

Sequence Viewer

Length: 795 bp
ATGTCATCCAACGCATCATCCACCCTCCACTTAAACCGCACGCCCGTTCTGGACCGTCGATTTCCGCTACGCGGTCTACCCACCCTATTTATTGTTCCCCATTCCTGTCACTCCATTCGCATTGAAAGCAGGAAGCTCCAATACGACGTCGTTGCGACCAAGGCCGTGCCTTTTAAATCCCCCACTACCTCCCGCACTGGCGGCACTGACAAGAACAACAACAACAACAGCAACAGCCTCAACCTTTCCGAAACGACAAGGAACACCAGCAACAACAACATGGGAAGCGAGGGTTGTGCCGCGGCTTCTGATTCGAGGAAACCCAGGTTTCTTTGCCTTCATGGGTTTCGAACCAGCGGTCTGATCATGAAGACCCAACTGGGTAAATGGCCGGAGTCGGTGCTCAACAATTTCGACCTGGACTTTCTCGATGGGCCTTTCCCGGCCCAAGGTAAATCCGAAGTCGAGGGCATTTTCGATCCTCCTTATCACGAGTGGTTCCAATTCAGCAAGGATTTTTCTGAGTACACGAACTTTGACGAGTGTCTTGAATTCATTGAAGATTACATGGTCAAGAATGGACCCTTTGATGGGCTACTTGGTTTTTCCCAGGGGGCAATACTGTCAGCGGCACTGCCGGGTCTACAAGCAAAGGTAATTGACAATGACCAAAAAGAACAAGGAAAAGAAAAGTTTCCGAATCTATATTGTGACACTGATATTGATAGGTATTTTGTTGTTTGTTATGGTAATTTGATGAGTTGTTTGACTTGTTATTGTAGGGTTTGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1

Protein Analysis

264

Amino Acids

29.72

Weight (kDa)

6.08

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 106 - 223 4e-31 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccI GTMKAC 2 cut(s) 76, 643
AccII CGCG 2 cut(s) 72, 302
AciI CCGC 9 cut(s) 37, 65, 72, 193, 201, 300, 302, 357, 629
AclWI GGATC 1 cut(s) 473
AcoI YGGCCR 1 cut(s) 389
AcsI RAATTY 1 cut(s) 551
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 1 cut(s) 527
AfiI CCNNNNNNNGG 4 cut(s) 71, 449, 590, 591
AgsI TTSAA 3 cut(s) 125, 551, 560
AjnI CCWGG 3 cut(s) 323, 417, 609
AluBI AGCT 1 cut(s) 136
AluI AGCT 1 cut(s) 136
Alw21I GWGCWC 1 cut(s) 405
AlwI GGATC 1 cut(s) 473
AoxI GGCC 4 cut(s) 162, 389, 434, 444
ApoI RAATTY 1 cut(s) 551
ArsI GACNNNNNNTTYG 2 cut(s) 343, 375
Asp700I GAANNNNTTC 1 cut(s) 693
AspS9I GGNCC 4 cut(s) 52, 434, 445, 581
AsuC2I CCSGG 2 cut(s) 443, 639
AsuII TTCGAA 1 cut(s) 349
AvaII GGWCC 2 cut(s) 52, 581
BarI GAAGNNNNNNTAC 2 cut(s) 125, 157
BauI CACGAG 1 cut(s) 491
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 1 cut(s) 405
BccI CCATC 2 cut(s) 425, 584
BceAI ACGGC 1 cut(s) 149
BcgI CGANNNNNNTGC 4 cut(s) 134, 168, 278, 312
BciT130I CCWGG 3 cut(s) 325, 419, 611
BclI TGATCA 1 cut(s) 363
BcnI CCSGG 2 cut(s) 443, 639
BisI GCNGC 4 cut(s) 202, 300, 303, 630
BlsI GCNGC 4 cut(s) 203, 301, 304, 631
Bme1390I CCNGG 5 cut(s) 325, 419, 443, 611, 639
Bme18I GGWCC 2 cut(s) 52, 581
BmgT120I GGNCC 4 cut(s) 52, 434, 445, 581
BmiI GGNNCC 2 cut(s) 500, 583
BmrFI CCNGG 5 cut(s) 325, 419, 443, 611, 639
BmrI ACTGGG 1 cut(s) 389
BmsI GCATC 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 389
BoxI GACNNNNGTC 1 cut(s) 543
BpiI GAAGAC 1 cut(s) 377
Bpu14I TTCGAA 1 cut(s) 349
BpuMI CCSGG 2 cut(s) 443, 639
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 6 cut(s) 159, 300, 323, 448, 609, 610
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 449, 590, 591
Bse1I ACTGG 2 cut(s) 202, 384
BseBI CCWGG 3 cut(s) 325, 419, 611
BseDI CCNNGG 6 cut(s) 159, 300, 323, 448, 609, 610
BseGI GGATG 2 cut(s) 5, 17
BseLI CCNNNNNNNGG 4 cut(s) 71, 449, 590, 591
BseMII CTCAG 1 cut(s) 513
BseNI ACTGG 2 cut(s) 202, 384
Bsh1236I CGCG 2 cut(s) 72, 302
BshFI GGCC 4 cut(s) 164, 391, 436, 446
BsiHKAI GWGCWC 1 cut(s) 405
BsiSI CCGG 3 cut(s) 392, 443, 638
BslI CCNNNNNNNGG 4 cut(s) 71, 449, 590, 591
BsnI GGCC 4 cut(s) 164, 391, 436, 446
Bsp119I TTCGAA 1 cut(s) 349
Bsp1286I GDGCHC 1 cut(s) 405
Bsp143I GATC 2 cut(s) 363, 478
BspACI CCGC 9 cut(s) 37, 65, 72, 193, 201, 300, 302, 357, 629
BspANI GGCC 4 cut(s) 164, 391, 436, 446
BspCNI CTCAG 1 cut(s) 514
BspFNI CGCG 2 cut(s) 72, 302
BspHI TCATGA 1 cut(s) 366
BspLI GGNNCC 2 cut(s) 500, 583
BspPI GGATC 1 cut(s) 473
BspT104I TTCGAA 1 cut(s) 349
BsrI ACTGG 2 cut(s) 202, 384
BssECI CCNNGG 6 cut(s) 159, 300, 323, 448, 609, 610
BssMI GATC 2 cut(s) 363, 478
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 1 cut(s) 491
BssT1I CCWWGG 2 cut(s) 159, 448
Bst2BI CACGAG 1 cut(s) 491
Bst2UI CCWGG 3 cut(s) 325, 419, 611
Bst4CI ACNGT 2 cut(s) 56, 624
BstACI GRCGYC 1 cut(s) 147
BstBI TTCGAA 1 cut(s) 349
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 1 cut(s) 522
BstDSI CCRYGG 1 cut(s) 300
BstF5I GGATG 2 cut(s) 5, 17
BstFNI CGCG 2 cut(s) 72, 302
BstKTI GATC 2 cut(s) 366, 481
BstMBI GATC 2 cut(s) 363, 478
BstMWI GCNNNNNNNGC 3 cut(s) 126, 161, 201
BstNI CCWGG 3 cut(s) 325, 419, 611
BstPAI GACNNNNGTC 1 cut(s) 543
BstSCI CCNGG 5 cut(s) 323, 417, 441, 609, 637
BstUI CGCG 2 cut(s) 72, 302
BstV2I GAAGAC 1 cut(s) 377
BsuRI GGCC 4 cut(s) 164, 391, 436, 446
BtgI CCRYGG 1 cut(s) 300
BtsCI GGATG 2 cut(s) 5, 17
BtsI GCAGTG 1 cut(s) 632
BtsIMutI CAGTG 4 cut(s) 195, 204, 632, 714
Cac8I GCNNGC 1 cut(s) 41
CciI TCATGA 1 cut(s) 366
Cfr13I GGNCC 4 cut(s) 52, 434, 445, 581
Cfr42I CCGCGG 1 cut(s) 303
Csp6I GTAC 1 cut(s) 526
CviAII CATG 4 cut(s) 280, 341, 367, 568
CviJI RGCY 8 cut(s) 136, 164, 237, 305, 391, 436, 446, 595
CviKI_1 RGCY 8 cut(s) 136, 164, 237, 305, 391, 436, 446, 595
CviQI GTAC 1 cut(s) 526
DdeI CTNAG 1 cut(s) 522
DpnI GATC 2 cut(s) 365, 480
DpnII GATC 2 cut(s) 363, 478
DraI TTTAAA 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 389
Eco130I CCWWGG 2 cut(s) 159, 448
Eco47I GGWCC 2 cut(s) 52, 581
EcoRI GAATTC 1 cut(s) 551
EcoRII CCWGG 3 cut(s) 323, 417, 609
EcoT14I CCWWGG 2 cut(s) 159, 448
ErhI CCWWGG 2 cut(s) 159, 448
FaeI CATG 4 cut(s) 283, 344, 370, 571
FaiI YATR 6 cut(s) 281, 342, 368, 569, 706, 747
FatI CATG 4 cut(s) 279, 340, 366, 567
FauI CCCGC 1 cut(s) 200
FbaI TGATCA 1 cut(s) 363
FblI GTMKAC 2 cut(s) 76, 643
Fnu4HI GCNGC 4 cut(s) 202, 300, 303, 630
FokI GGATG 1 cut(s) 4
Fsp4HI GCNGC 4 cut(s) 202, 300, 303, 630
GluI GCNGC 4 cut(s) 202, 300, 303, 630
HaeIII GGCC 4 cut(s) 164, 391, 436, 446
HapII CCGG 3 cut(s) 392, 443, 638
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 4 cut(s) 283, 344, 370, 571
HinfI GANTC 3 cut(s) 311, 395, 700
HpaII CCGG 3 cut(s) 392, 443, 638
Hpy166II GTNNAC 3 cut(s) 77, 528, 644
Hpy188I TCNGA 6 cut(s) 250, 310, 363, 460, 523, 699
Hpy188III TCNNGA 6 cut(s) 50, 367, 428, 491, 548, 574
Hpy8I GTNNAC 3 cut(s) 77, 528, 644
Hpy99I CGWCG 3 cut(s) 60, 149, 152
HpyAV CCTTC 1 cut(s) 347
HpyCH4III ACNGT 2 cut(s) 56, 624
HpyCH4IV ACGT 1 cut(s) 147
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 161, 201
HpyF3I CTNAG 1 cut(s) 522
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 4 cut(s) 283, 344, 370, 571
Ksp22I TGATCA 1 cut(s) 363
KspI CCGCGG 1 cut(s) 303
Kzo9I GATC 2 cut(s) 363, 478
LmnI GCTCC 1 cut(s) 141
LweI GCATC 1 cut(s) 23
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 2 cut(s) 107, 710
MalI GATC 2 cut(s) 365, 480
MboI GATC 2 cut(s) 363, 478
MboII GAAGA 2 cut(s) 382, 572
MhlI GDGCHC 1 cut(s) 405
MluCI AATT 5 cut(s) 409, 503, 551, 657, 751
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 7 cut(s) 35, 199, 248, 283, 309, 460, 492
MroXI GAANNNNTTC 1 cut(s) 693
MseI TTAA 2 cut(s) 32, 174
MspA1I CMGCKG 3 cut(s) 302, 357, 629
MspI CCGG 3 cut(s) 392, 443, 638
MspR9I CCNGG 5 cut(s) 325, 419, 443, 611, 639
MvaI CCWGG 3 cut(s) 325, 419, 611
MvnI CGCG 2 cut(s) 72, 302
MwoI GCNNNNNNNGC 3 cut(s) 126, 161, 201
NciI CCSGG 2 cut(s) 443, 639
NdeII GATC 2 cut(s) 363, 478
NlaIII CATG 4 cut(s) 283, 344, 370, 571
NlaIV GGNNCC 2 cut(s) 500, 583
NmuCI GTSAC 2 cut(s) 107, 710
NspV TTCGAA 1 cut(s) 349
PagI TCATGA 1 cut(s) 366
PasI CCCWGGG 1 cut(s) 610
PdmI GAANNNNTTC 1 cut(s) 693
PfeI GAWTC 2 cut(s) 311, 700
PkrI GCNGC 4 cut(s) 203, 301, 304, 631
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
PshAI GACNNNNGTC 1 cut(s) 543
Psp6I CCWGG 3 cut(s) 323, 417, 609
PspGI CCWGG 3 cut(s) 323, 417, 609
PspN4I GGNNCC 2 cut(s) 500, 583
PspPI GGNCC 4 cut(s) 52, 434, 445, 581
RsaI GTAC 1 cut(s) 527
RsaNI GTAC 1 cut(s) 526
SacII CCGCGG 1 cut(s) 303
SaqAI TTAA 2 cut(s) 32, 174
SatI GCNGC 4 cut(s) 202, 300, 303, 630
Sau3AI GATC 2 cut(s) 363, 478
Sau96I GGNCC 4 cut(s) 52, 434, 445, 581
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 5 cut(s) 325, 419, 443, 611, 639
SduI GDGCHC 1 cut(s) 405
SetI ASST 9 cut(s) 138, 150, 191, 246, 329, 420, 454, 657, 731
SfaNI GCATC 1 cut(s) 23
Sfr303I CCGCGG 1 cut(s) 303
SfuI TTCGAA 1 cut(s) 349
SgrBI CCGCGG 1 cut(s) 303
SinI GGWCC 2 cut(s) 52, 581
Sse9I AATT 5 cut(s) 409, 503, 551, 657, 751
SsiI CCGC 9 cut(s) 37, 65, 72, 193, 201, 300, 302, 357, 629
StyD4I CCNGG 5 cut(s) 323, 417, 441, 609, 637
StyI CCWWGG 2 cut(s) 159, 448
TaaI ACNGT 2 cut(s) 56, 624
TaiI ACGT 1 cut(s) 150
TaqI TCGA 7 cut(s) 58, 314, 349, 414, 429, 465, 477
TasI AATT 5 cut(s) 409, 503, 551, 657, 751
TatI WGTACW 1 cut(s) 525
TauI GCSGC 4 cut(s) 204, 302, 305, 632
TfiI GAWTC 2 cut(s) 311, 700
Tru1I TTAA 2 cut(s) 32, 174
Tru9I TTAA 2 cut(s) 32, 174
TscAI CASTG 4 cut(s) 202, 211, 639, 721
TseFI GTSAC 2 cut(s) 107, 710
Tsp45I GTSAC 2 cut(s) 107, 710
TspDTI ATGAA 3 cut(s) 329, 383, 544
TspRI CASTG 4 cut(s) 202, 211, 639, 721
VpaK11BI GGWCC 2 cut(s) 52, 581
XapI RAATTY 1 cut(s) 551
XmiI GTMKAC 2 cut(s) 76, 643
XmnI GAANNNNTTC 1 cut(s) 693
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.