Prupe.1G526700_v2.0.a1

Dihydrofolate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0008449
Physical Location & Seq
Forward (+)
43114086 .. 43116185
2100 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G526700.1

Sequence Viewer

Length: 957 bp
ATGATGATATCTCAATTTCGTTGGTCCGCTGCCGTTCACATTGAACGAGGCAGATTCCCACACGACGTCGTTCCAGTCAACAATAATTGCCTTGAACCTTCGCCTTTTAAATTCAGCCACCACGCCACTCAAATTCAATGCACATTTCATTCCTCCCACACTAGCGGAACTGAAAAGAAGAAGAAGAAGAAGAAAAAACTCATCGACCCATTGGCATATTCTTCTTCTTATTATTATTACAGAAAAACCCCAGAAAAACTAAGAAATTACATGGGTAGCCAGGGAAGCGAAGAAGGAGGGTTGTTGTTGAAGAAACCCAGATTTCTCTGCCTCCATGGATTCAGAACCAGTGGAGAAATCATGAAGAAACAAGTGGGTAAGTGGCCTGAATCTGTGCTGCAGAAGCTCGACCTTGTTTATCTTGATGGGCCTTTTCCGGCCCTAGGTAAATCTGACGTTGAGGGCATTTTTGATCCTCCTTATTATGAGTGGTTCCAATTCAACAAGGAATTTTCTGAGTACACAAACTTTGACAAGTGTTTGGAATACATAGAAGATTACATTATAAAGCAGGGACCATTTGATGGGCTAGTTGGTTTTTCCCAGGGTGCAATATTGTCAGCTGCGCTGCCGGGGCTACAAACGAAGGGGGTGGCATTGACTAAAGTACCTAAAATTAAATTCCTCATCATAATTGGAGGGGCCAAATTCAAGTCCCCAGCAGTGGCTGACGATGCTTATGCTTCTCCCATTCAATGCCCGTCCCTACACTTTTTAGGAGAGACGGACTTCTTGAAGCCATATGGACTTGAGCTGTTGGAACACTGTGTAGAACCTACAATCATTCATCATCCCAAGGGTCATACAGTCCCTAGGCTTGATGAAAAGGGTTTAGAGACCATGATGAGCTTCATTGACAAGATTCAAAAGGCATTGACTGAGAAAGACCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1

Protein Analysis

319

Amino Acids

36.09

Weight (kDa)

8.67

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 566
AatII GACGTC 1 cut(s) 69
AccB7I CCANNNNNTGG 1 cut(s) 584
AciI CCGC 2 cut(s) 27, 165
AclWI GGATC 1 cut(s) 467
AcsI RAATTY 5 cut(s) 110, 132, 509, 680, 707
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 2 cut(s) 521, 669
AfiI CCNNNNNNNGG 3 cut(s) 443, 584, 724
AgsI TTSAA 9 cut(s) 44, 95, 137, 310, 502, 712, 755, 796, 926
AjnI CCWGG 2 cut(s) 279, 603
AluBI AGCT 4 cut(s) 406, 623, 814, 909
AluI AGCT 4 cut(s) 406, 623, 814, 909
Alw26I GTCTC 2 cut(s) 776, 890
AlwI GGATC 1 cut(s) 467
AlwNI CAGNNNCTG 1 cut(s) 728
AoxI GGCC 4 cut(s) 383, 428, 438, 702
ApeKI GCWGC 4 cut(s) 29, 397, 623, 628
ApoI RAATTY 5 cut(s) 110, 132, 509, 680, 707
AspA2I CCTAGG 2 cut(s) 442, 872
AspLEI GCGC 1 cut(s) 628
AspS9I GGNCC 5 cut(s) 24, 428, 439, 575, 702
AsuC2I CCSGG 1 cut(s) 633
AvaII GGWCC 2 cut(s) 24, 575
AvrII CCTAGG 2 cut(s) 442, 872
BaeI ACNNNNGTAYC 2 cut(s) 651, 684
BbvI GCAGC 4 cut(s) 16, 384, 610, 615
BccI CCATC 2 cut(s) 419, 578
BceAI ACGGC 1 cut(s) 17
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BciT130I CCWGG 2 cut(s) 281, 605
BcnI CCSGG 1 cut(s) 633
BcoDI GTCTC 2 cut(s) 776, 890
BfaI CTAG 4 cut(s) 162, 443, 590, 873
BfmI CTRYAG 1 cut(s) 398
BisI GCNGC 4 cut(s) 30, 398, 624, 629
BlnI CCTAGG 2 cut(s) 442, 872
BlsI GCNGC 4 cut(s) 31, 399, 625, 630
Bme1390I CCNGG 3 cut(s) 281, 605, 633
Bme18I GGWCC 2 cut(s) 24, 575
BmgT120I GGNCC 5 cut(s) 24, 428, 439, 575, 702
BmiI GGNNCC 3 cut(s) 494, 576, 703
BmrFI CCNGG 3 cut(s) 281, 605, 633
BmsI GCATC 1 cut(s) 724
BpuEI CTTGAG 1 cut(s) 830
BpuMI CCSGG 1 cut(s) 633
BsaHI GRCGYC 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 890
BsaJI CCNNGG 8 cut(s) 280, 334, 442, 603, 604, 632, 855, 872
Bsc4I CCNNNNNNNGG 3 cut(s) 443, 584, 724
Bse1I ACTGG 2 cut(s) 74, 348
BseBI CCWGG 2 cut(s) 281, 605
BseDI CCNNGG 8 cut(s) 280, 334, 442, 603, 604, 632, 855, 872
BseGI GGATG 1 cut(s) 850
BseLI CCNNNNNNNGG 3 cut(s) 443, 584, 724
BseMII CTCAG 2 cut(s) 507, 930
BseNI ACTGG 2 cut(s) 74, 348
BseXI GCAGC 4 cut(s) 16, 384, 610, 615
BseYI CCCAGC 1 cut(s) 718
BshFI GGCC 4 cut(s) 385, 430, 440, 704
BsiSI CCGG 2 cut(s) 437, 632
BslFI GGGAC 4 cut(s) 588, 700, 748, 854
BslI CCNNNNNNNGG 3 cut(s) 443, 584, 724
BsmAI GTCTC 2 cut(s) 776, 890
BsmBI CGTCTC 1 cut(s) 776
BsmFI GGGAC 4 cut(s) 588, 700, 748, 854
BsnI GGCC 4 cut(s) 385, 430, 440, 704
Bso31I GGTCTC 1 cut(s) 890
Bsp143I GATC 1 cut(s) 472
Bsp19I CCATGG 1 cut(s) 334
BspACI CCGC 2 cut(s) 27, 165
BspANI GGCC 4 cut(s) 385, 430, 440, 704
BspCNI CTCAG 2 cut(s) 508, 931
BspHI TCATGA 1 cut(s) 360
BspLI GGNNCC 3 cut(s) 494, 576, 703
BspMAI CTGCAG 1 cut(s) 402
BspPI GGATC 1 cut(s) 467
BspTNI GGTCTC 1 cut(s) 890
BsrI ACTGG 2 cut(s) 74, 348
BssECI CCNNGG 8 cut(s) 280, 334, 442, 603, 604, 632, 855, 872
BssMI GATC 1 cut(s) 472
BssNI GRCGYC 1 cut(s) 66
BssT1I CCWWGG 4 cut(s) 334, 442, 855, 872
Bst2UI CCWGG 2 cut(s) 281, 605
Bst4CI ACNGT 3 cut(s) 827, 868, 954
BstACI GRCGYC 1 cut(s) 66
BstDEI CTNAG 3 cut(s) 260, 516, 939
BstDSI CCRYGG 1 cut(s) 334
BstF5I GGATG 1 cut(s) 850
BstHHI GCGC 1 cut(s) 628
BstKTI GATC 1 cut(s) 475
BstMAI GTCTC 2 cut(s) 776, 890
BstMBI GATC 1 cut(s) 472
BstMWI GCNNNNNNNGC 4 cut(s) 285, 403, 634, 734
BstNI CCWGG 2 cut(s) 281, 605
BstSCI CCNGG 3 cut(s) 279, 603, 631
BstSFI CTRYAG 1 cut(s) 398
BstV1I GCAGC 4 cut(s) 16, 384, 610, 615
BsuRI GGCC 4 cut(s) 385, 430, 440, 704
BtgI CCRYGG 1 cut(s) 334
BtsCI GGATG 1 cut(s) 850
BtsI GCAGTG 1 cut(s) 729
BtsIMutI CAGTG 3 cut(s) 355, 729, 823
CaiI CAGNNNCTG 1 cut(s) 728
CciI TCATGA 1 cut(s) 360
CfoI GCGC 1 cut(s) 628
Cfr13I GGNCC 5 cut(s) 24, 428, 439, 575, 702
Csp6I GTAC 2 cut(s) 520, 668
CviAII CATG 4 cut(s) 271, 335, 361, 901
CviQI GTAC 2 cut(s) 520, 668
DdeI CTNAG 3 cut(s) 260, 516, 939
DpnI GATC 1 cut(s) 474
DpnII GATC 1 cut(s) 472
DraI TTTAAA 1 cut(s) 109
Eco130I CCWWGG 4 cut(s) 334, 442, 855, 872
Eco31I GGTCTC 1 cut(s) 890
Eco32I GATATC 1 cut(s) 9
Eco47I GGWCC 2 cut(s) 24, 575
EcoRII CCWGG 2 cut(s) 279, 603
EcoRV GATATC 1 cut(s) 9
EcoT14I CCWWGG 4 cut(s) 334, 442, 855, 872
ErhI CCWWGG 4 cut(s) 334, 442, 855, 872
Esp3I CGTCTC 1 cut(s) 776
FaeI CATG 4 cut(s) 274, 338, 364, 904
FaqI GGGAC 4 cut(s) 588, 700, 748, 854
FatI CATG 4 cut(s) 270, 334, 360, 900
FauNDI CATATG 1 cut(s) 802
Fnu4HI GCNGC 4 cut(s) 30, 398, 624, 629
FokI GGATG 1 cut(s) 837
Fsp4HI GCNGC 4 cut(s) 30, 398, 624, 629
FspBI CTAG 4 cut(s) 162, 443, 590, 873
GlaI GCGC 1 cut(s) 627
GluI GCNGC 4 cut(s) 30, 398, 624, 629
GsaI CCCAGC 1 cut(s) 722
HaeIII GGCC 4 cut(s) 385, 430, 440, 704
HapII CCGG 2 cut(s) 437, 632
HhaI GCGC 1 cut(s) 628
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 4 cut(s) 274, 338, 364, 904
Hin6I GCGC 1 cut(s) 626
HinP1I GCGC 1 cut(s) 626
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 4 cut(s) 54, 339, 389, 922
HpaII CCGG 2 cut(s) 437, 632
Hpy166II GTNNAC 3 cut(s) 37, 79, 522
Hpy188I TCNGA 3 cut(s) 344, 454, 517
Hpy188III TCNNGA 3 cut(s) 361, 422, 793
Hpy8I GTNNAC 3 cut(s) 37, 79, 522
Hpy99I CGWCG 2 cut(s) 68, 71
HpyAV CCTTC 3 cut(s) 108, 287, 640
HpyCH4III ACNGT 3 cut(s) 827, 868, 954
HpyCH4IV ACGT 2 cut(s) 66, 456
HpyCH4V TGCA 3 cut(s) 141, 400, 611
HpyF10VI GCNNNNNNNGC 4 cut(s) 285, 403, 634, 734
HpyF3I CTNAG 3 cut(s) 260, 516, 939
HpySE526I ACGT 2 cut(s) 66, 456
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 4 cut(s) 274, 338, 364, 904
HspAI GCGC 1 cut(s) 626
Kzo9I GATC 1 cut(s) 472
Lsp1109I GCAGC 4 cut(s) 16, 384, 610, 615
LweI GCATC 1 cut(s) 724
MaeI CTAG 4 cut(s) 162, 443, 590, 873
MaeII ACGT 2 cut(s) 66, 456
MalI GATC 1 cut(s) 474
MboI GATC 1 cut(s) 472
MmeI TCCRAC 1 cut(s) 798
MnlI CCTC 8 cut(s) 41, 163, 290, 341, 454, 486, 692, 695
MseI TTAA 2 cut(s) 108, 678
MspA1I CMGCKG 2 cut(s) 29, 623
MspI CCGG 2 cut(s) 437, 632
MspR9I CCNGG 3 cut(s) 281, 605, 633
MvaI CCWGG 2 cut(s) 281, 605
MwoI GCNNNNNNNGC 4 cut(s) 285, 403, 634, 734
NciI CCSGG 1 cut(s) 633
NcoI CCATGG 1 cut(s) 334
NdeI CATATG 1 cut(s) 802
NdeII GATC 1 cut(s) 472
NlaIII CATG 4 cut(s) 274, 338, 364, 904
NlaIV GGNNCC 3 cut(s) 494, 576, 703
PagI TCATGA 1 cut(s) 360
PasI CCCWGGG 1 cut(s) 604
PfeI GAWTC 4 cut(s) 54, 339, 389, 922
PflMI CCANNNNNTGG 1 cut(s) 584
PkrI GCNGC 4 cut(s) 31, 399, 625, 630
PsiI TTATAA 1 cut(s) 566
Psp6I CCWGG 2 cut(s) 279, 603
PspFI CCCAGC 1 cut(s) 718
PspGI CCWGG 2 cut(s) 279, 603
PspN4I GGNNCC 3 cut(s) 494, 576, 703
PspPI GGNCC 5 cut(s) 24, 428, 439, 575, 702
PstI CTGCAG 1 cut(s) 402
PstNI CAGNNNCTG 1 cut(s) 728
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 2 cut(s) 521, 669
RsaNI GTAC 2 cut(s) 520, 668
SaqAI TTAA 2 cut(s) 108, 678
SatI GCNGC 4 cut(s) 30, 398, 624, 629
Sau3AI GATC 1 cut(s) 472
Sau96I GGNCC 5 cut(s) 24, 428, 439, 575, 702
ScrFI CCNGG 3 cut(s) 281, 605, 633
SfaNI GCATC 1 cut(s) 724
SfcI CTRYAG 1 cut(s) 398
SinI GGWCC 2 cut(s) 24, 575
SmlI CTYRAG 1 cut(s) 809
SmoI CTYRAG 1 cut(s) 809
SsiI CCGC 2 cut(s) 27, 165
SspI AATATT 1 cut(s) 615
SspMI CTAG 4 cut(s) 162, 443, 590, 873
StyD4I CCNGG 3 cut(s) 279, 603, 631
StyI CCWWGG 4 cut(s) 334, 442, 855, 872
TaaI ACNGT 3 cut(s) 827, 868, 954
TaiI ACGT 2 cut(s) 69, 459
TaqI TCGA 2 cut(s) 204, 408
TatI WGTACW 1 cut(s) 519
TfiI GAWTC 4 cut(s) 54, 339, 389, 922
Tru1I TTAA 2 cut(s) 108, 678
Tru9I TTAA 2 cut(s) 108, 678
TscAI CASTG 3 cut(s) 355, 729, 830
TseI GCWGC 4 cut(s) 29, 397, 623, 628
TspDTI ATGAA 5 cut(s) 137, 377, 836, 897, 901
TspGWI ACGGA 1 cut(s) 800
TspRI CASTG 3 cut(s) 355, 729, 830
Van91I CCANNNNNTGG 1 cut(s) 584
VpaK11BI GGWCC 2 cut(s) 24, 575
XapI RAATTY 5 cut(s) 110, 132, 509, 680, 707
XmaJI CCTAGG 2 cut(s) 442, 872
XspI CTAG 4 cut(s) 162, 443, 590, 873
ZraI GACGTC 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.