FvH4_5g37130

5'-adenylylsulfate reductase-like 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008540
Physical Location & Seq
Forward (+)
27216570 .. 27219493
2924 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g37130.t1

Sequence Viewer

Length: 951 bp
ATGGGGATTAGGGTTTGGGGATTTGGGATCCTGATTTTATTGATATGGGGCCGACTAACGTCCGCCGTTGAGCCGACGTCGTGCCCGACGAAGTCCGCGGCGGAGGCGATCTTTGGGTTCCGAGATTCCGTTTGTGCGATTAACGGCGTTACCAGATCCGTTGGTTCTAATGGTGTTATTGTGGGAGATGAGGTTTCATTACAAAGGGCATTGAATCTAGTTCACAAGAATAGTCATGAGTATGTGGCTGTACTCTTCTATGCATCATGGTGCCCTTTCTCAAGGATGTTTAAGCCAACATTTTCTGTTTTGGCTTCTTTGTACCCTTCCATTCCACATTTTGCGTTCGAAGAATCAGCCATCAGGCCAAGCATACTCTCCAAGTATGGAGTTCATGGTTTTCCTACACTTTTCATCTTGAATTCTACAATGCGAGTCCGATACCAGGGTGCCCGGACTCCTGGTTCTCTTGTTGCATTCTATAGTGATGTTACCGGCATCAAGACAGTATCATTGGATCAATTGTCACCGGAAAAAGTTGGACACCCACCAAATCATGAGAAGAATGATAGCAATGAGCAGGAAAGCTGCCCGTTTTCATGGGCAAGGTCCCCAGAGAATATGCTTCGTCAGGAAACATATTTGGCTCTCGCCTCTGCTTTTGTAATTCTGCGTTTGCTCTACATCTTTTTCCCCACTCTGATTTCTCTTGCTCAGTGCGCATGGAGAAGGCATATTCGAAACGTAAGATTCGGGAACTTGTTTGAGCACCCTGTGGCTTATCTAAAGCGAGCAGTGCAGTTGTTTAACTCTCTAAAAGAGCCTTGCAAAAGGAGTAATCTGCAGGAAGGAGCAATGAACGCACGGGTCTGGGCTTCCAAGTCGCTTGCCACAGTTTCTATTGGGGATGCAAACACCAGCAGGGGTACTGCAGTGACTGAAACTCGTTGA

Protein Analysis

317

Amino Acids

35.15

Weight (kDa)

9.55

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 77 - 160 6.3e-10 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 80
Acc16I TGCGCA 1 cut(s) 721
AccB1I GGYRCC 2 cut(s) 270, 449
AccII CGCG 1 cut(s) 98
AciI CCGC 4 cut(s) 63, 96, 98, 101
AclWI GGATC 4 cut(s) 22, 35, 150, 525
AcsI RAATTY 1 cut(s) 421
AcyI GRCGYC 1 cut(s) 77
AdeI CACNNNGTG 1 cut(s) 775
AfaI GTAC 3 cut(s) 252, 323, 928
AfiI CCNNNNNNNGG 1 cut(s) 445
AgsI TTSAA 2 cut(s) 214, 421
AjnI CCWGG 2 cut(s) 444, 460
AloI GAACNNNNNNTCC 2 cut(s) 448, 480
AluBI AGCT 1 cut(s) 588
AluI AGCT 1 cut(s) 588
Alw21I GWGCWC 1 cut(s) 771
AlwI GGATC 4 cut(s) 22, 35, 150, 525
AlwNI CAGNNNCTG 1 cut(s) 938
AoxI GGCC 2 cut(s) 49, 365
ApeKI GCWGC 1 cut(s) 588
ApoI RAATTY 1 cut(s) 421
AspLEI GCGC 1 cut(s) 722
AspS9I GGNCC 2 cut(s) 49, 609
AsuC2I CCSGG 1 cut(s) 454
AsuHPI GGTGA 1 cut(s) 519
AsuII TTCGAA 2 cut(s) 348, 739
AvaII GGWCC 1 cut(s) 609
BaeGI GKGCMC 3 cut(s) 86, 275, 454
BaeI ACNNNNGTAYC 4 cut(s) 433, 466, 918, 951
BamHI GGATCC 1 cut(s) 27
BanI GGYRCC 2 cut(s) 270, 449
Bbv12I GWGCWC 1 cut(s) 771
BbvI GCAGC 1 cut(s) 575
BccI CCATC 1 cut(s) 368
BceAI ACGGC 2 cut(s) 50, 160
BciT130I CCWGG 2 cut(s) 446, 462
BcnI CCSGG 1 cut(s) 454
BfaI CTAG 1 cut(s) 218
BfmI CTRYAG 3 cut(s) 481, 842, 930
BisI GCNGC 2 cut(s) 99, 589
BlsI GCNGC 2 cut(s) 100, 590
Bme1390I CCNGG 3 cut(s) 446, 454, 462
Bme18I GGWCC 1 cut(s) 609
BmgT120I GGNCC 2 cut(s) 49, 609
BmiI GGNNCC 6 cut(s) 29, 50, 119, 272, 451, 611
BmrFI CCNGG 3 cut(s) 446, 454, 462
BmsI GCATC 3 cut(s) 272, 507, 898
BoxI GACNNNNGTC 1 cut(s) 58
Bpu14I TTCGAA 2 cut(s) 348, 739
BpuEI CTTGAG 1 cut(s) 265
BpuMI CCSGG 1 cut(s) 454
BsaHI GRCGYC 1 cut(s) 77
BsaJI CCNNGG 2 cut(s) 96, 445
BsaWI WCCGGW 1 cut(s) 529
BsaXI ACNNNNNCTCC 2 cut(s) 177, 207
Bsc4I CCNNNNNNNGG 1 cut(s) 445
Bse118I RCCGGY 1 cut(s) 494
Bse3DI GCAATG 2 cut(s) 580, 861
BseBI CCWGG 2 cut(s) 446, 462
BseDI CCNNGG 2 cut(s) 96, 445
BseGI GGATG 2 cut(s) 291, 913
BseLI CCNNNNNNNGG 1 cut(s) 445
BseMI GCAATG 2 cut(s) 580, 861
BseMII CTCAG 1 cut(s) 728
BseSI GKGCMC 3 cut(s) 86, 275, 454
BseXI GCAGC 1 cut(s) 575
BsgI GTGCAG 1 cut(s) 818
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 2 cut(s) 51, 367
BshNI GGYRCC 2 cut(s) 270, 449
BsiHKAI GWGCWC 1 cut(s) 771
BsiSI CCGG 3 cut(s) 454, 495, 530
BslFI GGGAC 1 cut(s) 595
BslI CCNNNNNNNGG 1 cut(s) 445
BsmFI GGGAC 1 cut(s) 595
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 2 cut(s) 51, 367
Bsp119I TTCGAA 2 cut(s) 348, 739
Bsp1286I GDGCHC 4 cut(s) 86, 275, 454, 771
Bsp143I GATC 4 cut(s) 27, 108, 155, 517
BspACI CCGC 4 cut(s) 63, 96, 98, 101
BspANI GGCC 2 cut(s) 51, 367
BspCNI CTCAG 1 cut(s) 727
BspFNI CGCG 1 cut(s) 98
BspHI TCATGA 2 cut(s) 235, 556
BspLI GGNNCC 6 cut(s) 29, 50, 119, 272, 451, 611
BspMAI CTGCAG 2 cut(s) 846, 934
BspPI GGATC 4 cut(s) 22, 35, 150, 525
BspT104I TTCGAA 2 cut(s) 348, 739
BspT107I GGYRCC 2 cut(s) 270, 449
BsrDI GCAATG 2 cut(s) 580, 861
BsrFI RCCGGY 1 cut(s) 494
BssAI RCCGGY 1 cut(s) 494
BssECI CCNNGG 2 cut(s) 96, 445
BssMI GATC 4 cut(s) 27, 108, 155, 517
BssNI GRCGYC 1 cut(s) 77
Bst2UI CCWGG 2 cut(s) 446, 462
Bst4CI ACNGT 2 cut(s) 508, 895
Bst6I CTCTTC 1 cut(s) 260
BstACI GRCGYC 1 cut(s) 77
BstBI TTCGAA 2 cut(s) 348, 739
BstC8I GCNNGC 2 cut(s) 792, 888
BstDEI CTNAG 1 cut(s) 714
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 2 cut(s) 291, 913
BstFNI CGCG 1 cut(s) 98
BstHHI GCGC 1 cut(s) 722
BstKTI GATC 4 cut(s) 30, 111, 158, 520
BstMBI GATC 4 cut(s) 27, 108, 155, 517
BstMWI GCNNNNNNNGC 4 cut(s) 104, 719, 796, 860
BstNI CCWGG 2 cut(s) 446, 462
BstPAI GACNNNNGTC 1 cut(s) 58
BstSCI CCNGG 3 cut(s) 444, 452, 460
BstSFI CTRYAG 3 cut(s) 481, 842, 930
BstSLI GKGCMC 3 cut(s) 86, 275, 454
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 1 cut(s) 575
BstX2I RGATCY 2 cut(s) 27, 155
BstYI RGATCY 2 cut(s) 27, 155
BsuRI GGCC 2 cut(s) 51, 367
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 2 cut(s) 291, 913
BtsI GCAGTG 2 cut(s) 801, 939
BtsIMutI CAGTG 3 cut(s) 722, 801, 939
Cac8I GCNNGC 2 cut(s) 792, 888
CaiI CAGNNNCTG 1 cut(s) 938
CciI TCATGA 2 cut(s) 235, 556
CfoI GCGC 1 cut(s) 722
Cfr10I RCCGGY 1 cut(s) 494
Cfr13I GGNCC 2 cut(s) 49, 609
Cfr42I CCGCGG 1 cut(s) 99
Csp6I GTAC 3 cut(s) 251, 322, 927
CviAII CATG 6 cut(s) 236, 267, 395, 557, 600, 723
CviQI GTAC 3 cut(s) 251, 322, 927
DdeI CTNAG 1 cut(s) 714
DpnI GATC 4 cut(s) 29, 110, 157, 519
DpnII GATC 4 cut(s) 27, 108, 155, 517
DraIII CACNNNGTG 1 cut(s) 775
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
EciI GGCGGA 2 cut(s) 52, 116
Eco47I GGWCC 1 cut(s) 609
EcoO109I RGGNCCY 1 cut(s) 609
EcoRI GAATTC 1 cut(s) 421
EcoRII CCWGG 2 cut(s) 444, 460
EcoT22I ATGCAT 1 cut(s) 265
FaeI CATG 6 cut(s) 239, 270, 398, 560, 603, 726
FaqI GGGAC 1 cut(s) 595
FatI CATG 6 cut(s) 235, 266, 394, 556, 599, 722
Fnu4HI GCNGC 2 cut(s) 99, 589
FokI GGATG 2 cut(s) 298, 920
Fsp4HI GCNGC 2 cut(s) 99, 589
FspAI RTGCGCAY 1 cut(s) 721
FspBI CTAG 1 cut(s) 218
FspI TGCGCA 1 cut(s) 721
GlaI GCGC 1 cut(s) 721
GluI GCNGC 2 cut(s) 99, 589
HaeIII GGCC 2 cut(s) 51, 367
HapII CCGG 3 cut(s) 454, 495, 530
HhaI GCGC 1 cut(s) 722
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 6 cut(s) 239, 270, 398, 560, 603, 726
Hin6I GCGC 1 cut(s) 720
HinP1I GCGC 1 cut(s) 720
HinfI GANTC 6 cut(s) 125, 214, 353, 435, 457, 750
HpaII CCGG 3 cut(s) 454, 495, 530
HphI GGTGA 1 cut(s) 519
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 3 cut(s) 122, 440, 702
Hpy188III TCNNGA 7 cut(s) 31, 236, 418, 502, 557, 632, 754
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 3 cut(s) 79, 82, 91
HpyAV CCTTC 3 cut(s) 336, 723, 842
HpyCH4III ACNGT 2 cut(s) 508, 895
HpyCH4IV ACGT 3 cut(s) 59, 77, 744
HpyCH4V TGCA 7 cut(s) 263, 476, 799, 828, 844, 911, 932
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 719, 796, 860
HpyF3I CTNAG 1 cut(s) 714
HpySE526I ACGT 3 cut(s) 59, 77, 744
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 6 cut(s) 239, 270, 398, 560, 603, 726
HspAI GCGC 1 cut(s) 720
KspI CCGCGG 1 cut(s) 99
Kzo9I GATC 4 cut(s) 27, 108, 155, 517
LmnI GCTCC 1 cut(s) 851
Lsp1109I GCAGC 1 cut(s) 575
LweI GCATC 3 cut(s) 272, 507, 898
MaeI CTAG 1 cut(s) 218
MaeII ACGT 3 cut(s) 59, 77, 744
MaeIII GTNAC 4 cut(s) 148, 490, 525, 934
MalI GATC 4 cut(s) 29, 110, 157, 519
MboI GATC 4 cut(s) 27, 108, 155, 517
MboII GAAGA 3 cut(s) 247, 362, 574
MfeI CAATTG 1 cut(s) 521
MflI RGATCY 2 cut(s) 27, 155
MhlI GDGCHC 4 cut(s) 86, 275, 454, 771
MluCI AATT 3 cut(s) 421, 521, 666
MlyI GAGTC 2 cut(s) 444, 451
MmeI TCCRAC 1 cut(s) 520
MnlI CCTC 3 cut(s) 97, 184, 664
Mph1103I ATGCAT 1 cut(s) 265
MseI TTAA 3 cut(s) 141, 291, 807
MslI CAYNNNNRTG 2 cut(s) 240, 268
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 3 cut(s) 454, 495, 530
MspR9I CCNGG 3 cut(s) 446, 454, 462
MunI CAATTG 1 cut(s) 521
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 2 cut(s) 446, 462
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 4 cut(s) 104, 719, 796, 860
NciI CCSGG 1 cut(s) 454
NdeII GATC 4 cut(s) 27, 108, 155, 517
NlaIII CATG 6 cut(s) 239, 270, 398, 560, 603, 726
NlaIV GGNNCC 6 cut(s) 29, 50, 119, 272, 451, 611
NmuCI GTSAC 2 cut(s) 525, 934
NsbI TGCGCA 1 cut(s) 721
NsiI ATGCAT 1 cut(s) 265
NspV TTCGAA 2 cut(s) 348, 739
PagI TCATGA 2 cut(s) 235, 556
PcsI WCGNNNNNNNCGW 2 cut(s) 83, 86
PctI GAATGC 1 cut(s) 476
PfeI GAWTC 4 cut(s) 125, 214, 353, 750
PflFI GACNNNGTC 1 cut(s) 91
PkrI GCNGC 2 cut(s) 100, 590
PleI GAGTC 2 cut(s) 443, 451
PpsI GAGTC 2 cut(s) 443, 451
PpuMI RGGWCCY 1 cut(s) 609
PshAI GACNNNNGTC 1 cut(s) 58
Psp5II RGGWCCY 1 cut(s) 609
Psp6I CCWGG 2 cut(s) 444, 460
PspGI CCWGG 2 cut(s) 444, 460
PspN4I GGNNCC 6 cut(s) 29, 50, 119, 272, 451, 611
PspPI GGNCC 2 cut(s) 49, 609
PspPPI RGGWCCY 1 cut(s) 609
PstI CTGCAG 2 cut(s) 846, 934
PstNI CAGNNNCTG 1 cut(s) 938
PsuI RGATCY 2 cut(s) 27, 155
PsyI GACNNNGTC 1 cut(s) 91
RsaI GTAC 3 cut(s) 252, 323, 928
RsaNI GTAC 3 cut(s) 251, 322, 927
RseI CAYNNNNRTG 2 cut(s) 240, 268
SacII CCGCGG 1 cut(s) 99
SaqAI TTAA 3 cut(s) 141, 291, 807
SatI GCNGC 2 cut(s) 99, 589
Sau3AI GATC 4 cut(s) 27, 108, 155, 517
Sau96I GGNCC 2 cut(s) 49, 609
SchI GAGTC 2 cut(s) 444, 451
ScrFI CCNGG 3 cut(s) 446, 454, 462
SduI GDGCHC 4 cut(s) 86, 275, 454, 771
SetI ASST 6 cut(s) 62, 80, 195, 590, 611, 747
SfaNI GCATC 3 cut(s) 272, 507, 898
SfcI CTRYAG 3 cut(s) 481, 842, 930
Sfr303I CCGCGG 1 cut(s) 99
SfuI TTCGAA 2 cut(s) 348, 739
SgrBI CCGCGG 1 cut(s) 99
SinI GGWCC 1 cut(s) 609
SmiMI CAYNNNNRTG 2 cut(s) 240, 268
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
Sse9I AATT 3 cut(s) 421, 521, 666
SsiI CCGC 4 cut(s) 63, 96, 98, 101
SspMI CTAG 1 cut(s) 218
StyD4I CCNGG 3 cut(s) 444, 452, 460
TaaI ACNGT 2 cut(s) 508, 895
TaiI ACGT 3 cut(s) 62, 80, 747
TaqI TCGA 2 cut(s) 348, 739
TasI AATT 3 cut(s) 421, 521, 666
TatI WGTACW 1 cut(s) 250
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 4 cut(s) 125, 214, 353, 750
Tru1I TTAA 3 cut(s) 141, 291, 807
Tru9I TTAA 3 cut(s) 141, 291, 807
TscAI CASTG 3 cut(s) 722, 801, 939
TseFI GTSAC 2 cut(s) 525, 934
TseI GCWGC 1 cut(s) 588
Tsp45I GTSAC 2 cut(s) 525, 934
TspDTI ATGAA 5 cut(s) 186, 383, 403, 588, 872
TspGWI ACGGA 2 cut(s) 118, 148
TspRI CASTG 3 cut(s) 722, 801, 939
Tth111I GACNNNGTC 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 609
XapI RAATTY 1 cut(s) 421
XspI CTAG 1 cut(s) 218
ZraI GACGTC 1 cut(s) 78
Zsp2I ATGCAT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.