Rroxscaffold_3G00221610

5'-adenylylsulfate reductase-like 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008540
Physical Location & Seq
Reverse (-)
4377567 .. 4380287
2721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00221610.1

Sequence Viewer

Length: 951 bp
ATGGGGATTAGGGTTTGGGGATTTGGGATCCTGATATTATGGATATGGGGCCGGCTAACGTGCGCCGCCGAGCCGACGTCTTCTCCGGCGGAGTCCGCGGCGGAAGCGATCTTCGGATTCCGAGATTCGTATTGTCCGGCTAACGGCGTTACCAGATCCGTTGGTTCTAATGGTGTTATTGTGGGAGATGAGGTTTCGTTACAAAGGGCATTGAATCTGGTTCACAAGAATAGTCACGAGTATGTGGCTGTGCTCTTCTATGCATCTTGGTGCCCTTTCTCAAGGATGTTTAAGCCAACATTTTCTGTTCTGGCTTCTTTGTATCCTTCCATCCCACATTTTGCATTTGAAGAATCAGCCATCAGGCCAAGTATACTCTCAAAGTATGGAGTTCATGGCTTTCCTACTCTTTTCATTTTGAATTCTACCATGCGAGTTCGATACCAGGGCGCCCGGACTCCTGGTTCTCTTATTGCTTTCTATAGTGAAGTTACTGGCATCAAGACAGTATCATTGGATCAATTGTCACTGGAAAAGGCCGGACACCCACCAAATCATGAGAAGAATGATAGCAATGAGCAGGAAAGCTGCCCCTTTTCTTGGGCAAGATCTCCAGAGAATTTGCTTCGTCAGGAAACATATTTGGCTCTTGCCTCTGCTTTTGTAATTCTGCGGTTGCTCTACATCTTTTTTCCCACTCTGATTTCATTTGCTCAATCTGCATGGAGAAGGCATATCCGAAATGTAAGATTCGGGAGCTTGTTTGAGCACCCTGTGGCCTATTTAAAGCGTGCAGTGCAGTTGTTTAACTCTCTAAAAGAGCCTTGCAAAAGGAGTAATCTGCAGGAAGGAGCAATGAACGCCCGGGTCTGGGCTTCCAAGTCGCTCGCCACAGTTTCCATCGGGGATGCAAGTACCAGCAGGGGTACCGCAGTGACTGAAACCCGTTGA

Protein Analysis

316

Amino Acids

35.18

Weight (kDa)

9.44

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 77 - 160 6.6e-10 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 80
Acc65I GGTACC 1 cut(s) 926
AccB1I GGYRCC 3 cut(s) 270, 449, 926
AccI GTMKAC 1 cut(s) 373
AccII CGCG 1 cut(s) 98
AciI CCGC 7 cut(s) 66, 89, 96, 98, 101, 673, 930
AclWI GGATC 4 cut(s) 22, 35, 150, 525
AcsI RAATTY 2 cut(s) 421, 619
AcyI GRCGYC 2 cut(s) 77, 450
AdeI CACNNNGTG 1 cut(s) 775
AfaI GTAC 2 cut(s) 916, 928
AfiI CCNNNNNNNGG 4 cut(s) 143, 600, 870, 871
AgsI TTSAA 3 cut(s) 214, 350, 421
AjnI CCWGG 2 cut(s) 444, 460
AloI GAACNNNNNNTCC 2 cut(s) 448, 480
AluBI AGCT 2 cut(s) 588, 759
AluI AGCT 2 cut(s) 588, 759
Alw21I GWGCWC 2 cut(s) 255, 771
AlwI GGATC 4 cut(s) 22, 35, 150, 525
AlwNI CAGNNNCTG 1 cut(s) 938
Ama87I CYCGRG 1 cut(s) 864
AoxI GGCC 4 cut(s) 49, 365, 537, 777
ApeKI GCWGC 1 cut(s) 588
ApoI RAATTY 2 cut(s) 421, 619
Asp718I GGTACC 1 cut(s) 926
AspLEI GCGC 2 cut(s) 65, 452
AspS9I GGNCC 1 cut(s) 49
AsuC2I CCSGG 3 cut(s) 454, 865, 866
AvaI CYCGRG 1 cut(s) 864
BaeGI GKGCMC 1 cut(s) 275
BaeI ACNNNNGTAYC 2 cut(s) 918, 951
BamHI GGATCC 1 cut(s) 27
BanI GGYRCC 3 cut(s) 270, 449, 926
BauI CACGAG 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 2 cut(s) 255, 771
BbvI GCAGC 1 cut(s) 575
BccI CCATC 3 cut(s) 338, 368, 908
BceAI ACGGC 1 cut(s) 160
BciT130I CCWGG 2 cut(s) 446, 462
BciVI GTATCC 1 cut(s) 333
BcnI CCSGG 3 cut(s) 454, 865, 866
BfmI CTRYAG 2 cut(s) 481, 842
BfoI RGCGCY 1 cut(s) 453
BfuI GTATCC 1 cut(s) 333
BglII AGATCT 1 cut(s) 608
BisI GCNGC 3 cut(s) 66, 99, 589
BlsI GCNGC 3 cut(s) 67, 100, 590
Bme1390I CCNGG 5 cut(s) 446, 454, 462, 865, 866
BmeT110I CYCGRG 1 cut(s) 864
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 5 cut(s) 29, 50, 272, 451, 928
BmrFI CCNGG 5 cut(s) 446, 454, 462, 865, 866
BmsI GCATC 3 cut(s) 272, 507, 898
BpiI GAAGAC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 597
BpuEI CTTGAG 1 cut(s) 265
BpuMI CCSGG 3 cut(s) 454, 865, 866
BsaHI GRCGYC 2 cut(s) 77, 450
BsaJI CCNNGG 3 cut(s) 96, 445, 864
BsaXI ACNNNNNCTCC 4 cut(s) 67, 97, 177, 207
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 600, 870, 871
Bse118I RCCGGY 1 cut(s) 51
Bse1I ACTGG 2 cut(s) 499, 534
Bse3DI GCAATG 2 cut(s) 580, 861
BseBI CCWGG 2 cut(s) 446, 462
BseDI CCNNGG 3 cut(s) 96, 445, 864
BseGI GGATG 3 cut(s) 291, 330, 913
BseLI CCNNNNNNNGG 4 cut(s) 143, 600, 870, 871
BseMI GCAATG 2 cut(s) 580, 861
BseNI ACTGG 2 cut(s) 499, 534
BseSI GKGCMC 1 cut(s) 275
BseXI GCAGC 1 cut(s) 575
BsgI GTGCAG 2 cut(s) 813, 818
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 4 cut(s) 51, 367, 539, 779
BshNI GGYRCC 3 cut(s) 270, 449, 926
BsiHKAI GWGCWC 2 cut(s) 255, 771
BsiHKCI CYCGRG 1 cut(s) 864
BsiSI CCGG 6 cut(s) 52, 86, 137, 454, 540, 865
BslI CCNNNNNNNGG 4 cut(s) 143, 600, 870, 871
BsnI GGCC 4 cut(s) 51, 367, 539, 779
BsoBI CYCGRG 1 cut(s) 864
Bsp1286I GDGCHC 3 cut(s) 255, 275, 771
Bsp143I GATC 5 cut(s) 27, 108, 155, 517, 608
BspACI CCGC 7 cut(s) 66, 89, 96, 98, 101, 673, 930
BspANI GGCC 4 cut(s) 51, 367, 539, 779
BspFNI CGCG 1 cut(s) 98
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 5 cut(s) 29, 50, 272, 451, 928
BspMAI CTGCAG 1 cut(s) 846
BspPI GGATC 4 cut(s) 22, 35, 150, 525
BspQI GCTCTTC 1 cut(s) 260
BspT107I GGYRCC 3 cut(s) 270, 449, 926
BsrDI GCAATG 2 cut(s) 580, 861
BsrFI RCCGGY 1 cut(s) 51
BsrI ACTGG 2 cut(s) 499, 534
BssAI RCCGGY 1 cut(s) 51
BssECI CCNNGG 3 cut(s) 96, 445, 864
BssMI GATC 5 cut(s) 27, 108, 155, 517, 608
BssNAI GTATAC 1 cut(s) 374
BssNI GRCGYC 2 cut(s) 77, 450
BssSI CACGAG 1 cut(s) 236
Bst1107I GTATAC 1 cut(s) 374
Bst2BI CACGAG 1 cut(s) 236
Bst2UI CCWGG 2 cut(s) 446, 462
Bst4CI ACNGT 2 cut(s) 508, 895
Bst6I CTCTTC 1 cut(s) 260
BstACI GRCGYC 2 cut(s) 77, 450
BstC8I GCNNGC 3 cut(s) 53, 792, 888
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 3 cut(s) 291, 330, 913
BstFNI CGCG 1 cut(s) 98
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 2 cut(s) 65, 452
BstKTI GATC 5 cut(s) 30, 111, 158, 520, 611
BstMBI GATC 5 cut(s) 27, 108, 155, 517, 608
BstMWI GCNNNNNNNGC 5 cut(s) 95, 104, 719, 796, 860
BstNI CCWGG 2 cut(s) 446, 462
BstSCI CCNGG 5 cut(s) 444, 452, 460, 863, 864
BstSFI CTRYAG 2 cut(s) 481, 842
BstSLI GKGCMC 1 cut(s) 275
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 1 cut(s) 575
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 3 cut(s) 27, 155, 608
BstYI RGATCY 3 cut(s) 27, 155, 608
BstZ17I GTATAC 1 cut(s) 374
BsuI GTATCC 1 cut(s) 333
BsuRI GGCC 4 cut(s) 51, 367, 539, 779
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 3 cut(s) 291, 330, 913
BtsI GCAGTG 2 cut(s) 801, 939
BtsIMutI CAGTG 3 cut(s) 527, 801, 939
Cac8I GCNNGC 3 cut(s) 53, 792, 888
CaiI CAGNNNCTG 1 cut(s) 938
CciI TCATGA 1 cut(s) 556
CfoI GCGC 2 cut(s) 65, 452
Cfr10I RCCGGY 1 cut(s) 51
Cfr13I GGNCC 1 cut(s) 49
Cfr42I CCGCGG 1 cut(s) 99
Cfr9I CCCGGG 1 cut(s) 864
Csp6I GTAC 2 cut(s) 915, 927
CviAII CATG 4 cut(s) 395, 430, 557, 723
CviQI GTAC 2 cut(s) 915, 927
DinI GGCGCC 1 cut(s) 451
DpnI GATC 5 cut(s) 29, 110, 157, 519, 610
DpnII GATC 5 cut(s) 27, 108, 155, 517, 608
DraI TTTAAA 1 cut(s) 786
DraIII CACNNNGTG 1 cut(s) 775
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
EciI GGCGGA 2 cut(s) 104, 116
Eco88I CYCGRG 1 cut(s) 864
EcoRI GAATTC 1 cut(s) 421
EcoRII CCWGG 2 cut(s) 444, 460
EcoT22I ATGCAT 1 cut(s) 265
EgeI GGCGCC 1 cut(s) 451
EheI GGCGCC 1 cut(s) 451
FaeI CATG 4 cut(s) 398, 433, 560, 726
FatI CATG 4 cut(s) 394, 429, 556, 722
FblI GTMKAC 1 cut(s) 373
Fnu4HI GCNGC 3 cut(s) 66, 99, 589
FokI GGATG 3 cut(s) 298, 317, 920
Fsp4HI GCNGC 3 cut(s) 66, 99, 589
GlaI GCGC 2 cut(s) 64, 451
GluI GCNGC 3 cut(s) 66, 99, 589
GsuI CTGGAG 1 cut(s) 597
HaeII RGCGCY 1 cut(s) 453
HaeIII GGCC 4 cut(s) 51, 367, 539, 779
HapII CCGG 6 cut(s) 52, 86, 137, 454, 540, 865
HhaI GCGC 2 cut(s) 65, 452
Hin1I GRCGYC 2 cut(s) 77, 450
Hin1II CATG 4 cut(s) 398, 433, 560, 726
Hin6I GCGC 2 cut(s) 63, 450
HinP1I GCGC 2 cut(s) 63, 450
HinfI GANTC 7 cut(s) 92, 117, 125, 214, 353, 457, 750
HpaII CCGG 6 cut(s) 52, 86, 137, 454, 540, 865
Hpy166II GTNNAC 2 cut(s) 223, 374
Hpy188I TCNGA 4 cut(s) 116, 122, 702, 740
Hpy188III TCNNGA 7 cut(s) 31, 236, 502, 557, 614, 632, 754
Hpy8I GTNNAC 2 cut(s) 223, 374
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 3 cut(s) 336, 723, 842
HpyCH4III ACNGT 2 cut(s) 508, 895
HpyCH4IV ACGT 2 cut(s) 59, 77
HpyCH4V TGCA 8 cut(s) 263, 344, 722, 794, 799, 828, 844, 911
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 104, 719, 796, 860
HpySE526I ACGT 2 cut(s) 59, 77
Hsp92I GRCGYC 2 cut(s) 77, 450
Hsp92II CATG 4 cut(s) 398, 433, 560, 726
HspAI GCGC 2 cut(s) 63, 450
KasI GGCGCC 1 cut(s) 449
KpnI GGTACC 1 cut(s) 930
KroI GCCGGC 1 cut(s) 51
KroNI GCCGGC 1 cut(s) 53
KspI CCGCGG 1 cut(s) 99
Kzo9I GATC 5 cut(s) 27, 108, 155, 517, 608
LguI GCTCTTC 1 cut(s) 260
LmnI GCTCC 2 cut(s) 756, 851
Lsp1109I GCAGC 1 cut(s) 575
LweI GCATC 3 cut(s) 272, 507, 898
MaeII ACGT 2 cut(s) 59, 77
MaeIII GTNAC 6 cut(s) 148, 198, 233, 490, 525, 934
MalI GATC 5 cut(s) 29, 110, 157, 519, 610
MboI GATC 5 cut(s) 27, 108, 155, 517, 608
MboII GAAGA 5 cut(s) 72, 103, 247, 362, 574
MfeI CAATTG 1 cut(s) 521
MflI RGATCY 3 cut(s) 27, 155, 608
MhlI GDGCHC 3 cut(s) 255, 275, 771
MluCI AATT 4 cut(s) 421, 521, 619, 666
Mly113I GGCGCC 1 cut(s) 450
MlyI GAGTC 2 cut(s) 101, 451
MnlI CCTC 2 cut(s) 184, 664
Mph1103I ATGCAT 1 cut(s) 265
MroNI GCCGGC 1 cut(s) 51
MseI TTAA 3 cut(s) 291, 785, 807
MslI CAYNNNNRTG 2 cut(s) 240, 268
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 6 cut(s) 52, 86, 137, 454, 540, 865
MspR9I CCNGG 5 cut(s) 446, 454, 462, 865, 866
MunI CAATTG 1 cut(s) 521
MvaI CCWGG 2 cut(s) 446, 462
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 5 cut(s) 95, 104, 719, 796, 860
NaeI GCCGGC 1 cut(s) 53
NarI GGCGCC 1 cut(s) 450
NciI CCSGG 3 cut(s) 454, 865, 866
NdeII GATC 5 cut(s) 27, 108, 155, 517, 608
NgoMIV GCCGGC 1 cut(s) 51
NlaIII CATG 4 cut(s) 398, 433, 560, 726
NlaIV GGNNCC 5 cut(s) 29, 50, 272, 451, 928
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 3 cut(s) 233, 525, 934
NsiI ATGCAT 1 cut(s) 265
PagI TCATGA 1 cut(s) 556
PciSI GCTCTTC 1 cut(s) 260
PdiI GCCGGC 1 cut(s) 53
PfeI GAWTC 5 cut(s) 117, 125, 214, 353, 750
PkrI GCNGC 3 cut(s) 67, 100, 590
PleI GAGTC 2 cut(s) 100, 451
PluTI GGCGCC 1 cut(s) 453
PpsI GAGTC 2 cut(s) 100, 451
Psp6I CCWGG 2 cut(s) 444, 460
PspGI CCWGG 2 cut(s) 444, 460
PspN4I GGNNCC 5 cut(s) 29, 50, 272, 451, 928
PspPI GGNCC 1 cut(s) 49
PstI CTGCAG 1 cut(s) 846
PstNI CAGNNNCTG 1 cut(s) 938
PsuI RGATCY 3 cut(s) 27, 155, 608
RsaI GTAC 2 cut(s) 916, 928
RsaNI GTAC 2 cut(s) 915, 927
RseI CAYNNNNRTG 2 cut(s) 240, 268
SacII CCGCGG 1 cut(s) 99
SapI GCTCTTC 1 cut(s) 260
SaqAI TTAA 3 cut(s) 291, 785, 807
SatI GCNGC 3 cut(s) 66, 99, 589
Sau3AI GATC 5 cut(s) 27, 108, 155, 517, 608
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 2 cut(s) 101, 451
ScrFI CCNGG 5 cut(s) 446, 454, 462, 865, 866
SduI GDGCHC 3 cut(s) 255, 275, 771
SetI ASST 5 cut(s) 62, 80, 195, 590, 761
SfaNI GCATC 3 cut(s) 272, 507, 898
SfcI CTRYAG 2 cut(s) 481, 842
SfoI GGCGCC 1 cut(s) 451
Sfr303I CCGCGG 1 cut(s) 99
SgrBI CCGCGG 1 cut(s) 99
SmaI CCCGGG 1 cut(s) 866
SmiMI CAYNNNNRTG 2 cut(s) 240, 268
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
Sse9I AATT 4 cut(s) 421, 521, 619, 666
SsiI CCGC 7 cut(s) 66, 89, 96, 98, 101, 673, 930
SspDI GGCGCC 1 cut(s) 449
StyD4I CCNGG 5 cut(s) 444, 452, 460, 863, 864
TaaI ACNGT 2 cut(s) 508, 895
TaiI ACGT 2 cut(s) 62, 80
TaqI TCGA 1 cut(s) 439
TasI AATT 4 cut(s) 421, 521, 619, 666
TauI GCSGC 2 cut(s) 68, 101
TfiI GAWTC 5 cut(s) 117, 125, 214, 353, 750
Tru1I TTAA 3 cut(s) 291, 785, 807
Tru9I TTAA 3 cut(s) 291, 785, 807
TscAI CASTG 3 cut(s) 534, 801, 939
TseFI GTSAC 3 cut(s) 233, 525, 934
TseI GCWGC 1 cut(s) 588
Tsp45I GTSAC 3 cut(s) 233, 525, 934
TspDTI ATGAA 4 cut(s) 383, 403, 696, 872
TspGWI ACGGA 1 cut(s) 148
TspMI CCCGGG 1 cut(s) 864
TspRI CASTG 3 cut(s) 534, 801, 939
XapI RAATTY 2 cut(s) 421, 619
XmaI CCCGGG 1 cut(s) 864
XmiI GTMKAC 1 cut(s) 373
ZraI GACGTC 1 cut(s) 78
Zsp2I ATGCAT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.