MD08G1184200.v1.1

5'-adenylylsulfate reductase-like 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008540
Physical Location & Seq
Reverse (-)
22946721 .. 22949465
2745 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1184200.v1.1.491

Sequence Viewer

Length: 942 bp
ATGGGGATTAGGGTTTGGGAAAGTGGGATCGTGATATTGGTTTTATGGGTCCGGCTAGTTTGCGCCGCCGAGCCCGCCTCTTGCCCGACGGAGTCCGTTGCCGACACGATCTTCGGCTTCCGGTATTCCAATTGCCCCGTCGACGGCGCTTGCAGATCCGTTGAATCCATGGGTGTTATTGTGGGAGATGAGGTTTCCTTACAAAGGGCATTAAATATGGTTCACAAGAATACTCATGATTATGTGGCGGTGCTCTTCTATGCGTCATGGTGCCCTTTTTCAAGGATGTTTAAACCAACGTTTTCCATCCTGGCTTCTTTGTATCCTTCGATCCCCCATTTTGCATTTGAAGAATCAGCCATCAGGCCAAGTATACTCTCCAAATATGGAGTTCATGGATTTCCTACACTTTTCATTTTGAATTCTACTATGCGTATTCGATATCAAGGCGCCCGCACTCCTGGTTCTCTTATTGCTTTCTATAGTCATGTTACTGGCTTCAAGACTGTATCACTGGATCAATTGTCACTGGAAAAAATTGGATACCCACTAAATCATGAGAGGCATGATAGCACTGAGCAGGAAAGCTGCCCATTTTCTTGGGCTAGATCTCCAGAGAATTTGCTTCGGCAAGAAACATATTTGGCTCTGGCTTCTGCTTTTGTGCTTCTGAGATTGCTCTACTTCTTTTTTCCTGCTCTGCTATCGTTTGCTCAATCTGCATGGAGATGGCATATTCAAGACATGAGATTAGGGAGCTTGTTGGAGCACCCTTTGGCTTTTCTAAAGCGGGCAGTGCAGTTGTTTAATTCTCTAAAAGAGCCTTGCAAGAGGAGTAATTTGCAGGAAGGAGCAATGAACGCCAGGGTGTGGGCTTCCAAGTCACTTGCCACAGTTTCTATCGGGGATGCAAGCACCAGCAGGGGTTATAGCACTGACTGA

Protein Analysis

314

Amino Acids

35.17

Weight (kDa)

8.65

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 77 - 160 1.6e-09 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 270, 449
AccB7I CCANNNNNTGG 1 cut(s) 870
AccI GTMKAC 2 cut(s) 141, 373
AciI CCGC 5 cut(s) 66, 75, 248, 454, 790
AclI AACGTT 1 cut(s) 299
AclWI GGATC 4 cut(s) 35, 150, 325, 525
AcsI RAATTY 2 cut(s) 421, 619
AcyI GRCGYC 1 cut(s) 450
AfiI CCNNNNNNNGG 3 cut(s) 143, 204, 870
AgsI TTSAA 6 cut(s) 164, 282, 350, 421, 502, 740
AjnI CCWGG 3 cut(s) 309, 460, 863
AluBI AGCT 2 cut(s) 588, 759
AluI AGCT 2 cut(s) 588, 759
Alw21I GWGCWC 2 cut(s) 255, 771
AlwI GGATC 4 cut(s) 35, 150, 325, 525
AoxI GGCC 1 cut(s) 365
ApeKI GCWGC 1 cut(s) 588
ApoI RAATTY 2 cut(s) 421, 619
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
AspLEI GCGC 3 cut(s) 65, 149, 452
AspS9I GGNCC 1 cut(s) 49
AvaII GGWCC 1 cut(s) 49
BaeGI GKGCMC 1 cut(s) 275
BanI GGYRCC 2 cut(s) 270, 449
BanII GRGCYC 1 cut(s) 75
Bbv12I GWGCWC 2 cut(s) 255, 771
BbvI GCAGC 1 cut(s) 575
BccI CCATC 3 cut(s) 314, 368, 723
BceAI ACGGC 1 cut(s) 160
BciT130I CCWGG 3 cut(s) 311, 462, 865
BciVI GTATCC 2 cut(s) 333, 536
BfaI CTAG 2 cut(s) 56, 606
BfmI CTRYAG 1 cut(s) 481
BfoI RGCGCY 2 cut(s) 150, 453
BfuI GTATCC 2 cut(s) 333, 536
BglII AGATCT 1 cut(s) 608
BisI GCNGC 2 cut(s) 66, 589
BlsI GCNGC 2 cut(s) 67, 590
Bme1390I CCNGG 3 cut(s) 311, 462, 865
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 3 cut(s) 50, 272, 451
BmrFI CCNGG 3 cut(s) 311, 462, 865
BmsI GCATC 1 cut(s) 898
BplI GAGNNNNNCTC 2 cut(s) 62, 94
BpmI CTGGAG 1 cut(s) 597
BsaHI GRCGYC 1 cut(s) 450
BsaJI CCNNGG 2 cut(s) 168, 864
BsaWI WCCGGW 1 cut(s) 120
BsaXI ACNNNNNCTCC 2 cut(s) 177, 207
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 204, 870
Bse1I ACTGG 3 cut(s) 499, 519, 534
Bse3DI GCAATG 1 cut(s) 861
BseBI CCWGG 3 cut(s) 311, 462, 865
BseDI CCNNGG 2 cut(s) 168, 864
BseGI GGATG 3 cut(s) 291, 306, 913
BseLI CCNNNNNNNGG 3 cut(s) 143, 204, 870
BseMI GCAATG 1 cut(s) 861
BseMII CTCAG 2 cut(s) 567, 662
BseNI ACTGG 3 cut(s) 499, 519, 534
BseRI GAGGAG 1 cut(s) 847
BseSI GKGCMC 1 cut(s) 275
BseXI GCAGC 1 cut(s) 575
BsgI GTGCAG 1 cut(s) 818
BshFI GGCC 1 cut(s) 367
BshNI GGYRCC 2 cut(s) 270, 449
BsiHKAI GWGCWC 2 cut(s) 255, 771
BsiSI CCGG 2 cut(s) 52, 121
BslI CCNNNNNNNGG 3 cut(s) 143, 204, 870
BsnI GGCC 1 cut(s) 367
Bsp1286I GDGCHC 4 cut(s) 75, 255, 275, 771
Bsp143I GATC 6 cut(s) 27, 108, 155, 330, 517, 608
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 5 cut(s) 66, 75, 248, 454, 790
BspANI GGCC 1 cut(s) 367
BspCNI CTCAG 2 cut(s) 568, 663
BspHI TCATGA 2 cut(s) 235, 556
BspLI GGNNCC 3 cut(s) 50, 272, 451
BspPI GGATC 4 cut(s) 35, 150, 325, 525
BspQI GCTCTTC 1 cut(s) 260
BspT107I GGYRCC 2 cut(s) 270, 449
BsrDI GCAATG 1 cut(s) 861
BsrI ACTGG 3 cut(s) 499, 519, 534
BssECI CCNNGG 2 cut(s) 168, 864
BssMI GATC 6 cut(s) 27, 108, 155, 330, 517, 608
BssNAI GTATAC 1 cut(s) 374
BssNI GRCGYC 1 cut(s) 450
BssT1I CCWWGG 1 cut(s) 168
Bst1107I GTATAC 1 cut(s) 374
Bst2UI CCWGG 3 cut(s) 311, 462, 865
Bst4CI ACNGT 2 cut(s) 508, 895
Bst6I CTCTTC 1 cut(s) 260
BstACI GRCGYC 1 cut(s) 450
BstC8I GCNNGC 5 cut(s) 75, 151, 454, 792, 913
BstDEI CTNAG 2 cut(s) 576, 671
BstDSI CCRYGG 1 cut(s) 168
BstENI CCTNNNNNAGG 1 cut(s) 202
BstF5I GGATG 3 cut(s) 291, 306, 913
BstH2I RGCGCY 2 cut(s) 150, 453
BstHHI GCGC 3 cut(s) 65, 149, 452
BstKTI GATC 6 cut(s) 30, 111, 158, 333, 520, 611
BstMBI GATC 6 cut(s) 27, 108, 155, 330, 517, 608
BstMWI GCNNNNNNNGC 4 cut(s) 74, 719, 796, 860
BstNI CCWGG 3 cut(s) 311, 462, 865
BstSCI CCNGG 3 cut(s) 309, 460, 863
BstSFI CTRYAG 1 cut(s) 481
BstSLI GKGCMC 1 cut(s) 275
BstV1I GCAGC 1 cut(s) 575
BstX2I RGATCY 2 cut(s) 155, 608
BstXI CCANNNNNNTGG 1 cut(s) 600
BstYI RGATCY 2 cut(s) 155, 608
BstZ17I GTATAC 1 cut(s) 374
BsuI GTATCC 2 cut(s) 333, 536
BsuRI GGCC 1 cut(s) 367
BtgI CCRYGG 1 cut(s) 168
BtsCI GGATG 3 cut(s) 291, 306, 913
BtsI GCAGTG 1 cut(s) 801
BtsIMutI CAGTG 5 cut(s) 512, 527, 573, 801, 933
Cac8I GCNNGC 5 cut(s) 75, 151, 454, 792, 913
CciI TCATGA 2 cut(s) 235, 556
CfoI GCGC 3 cut(s) 65, 149, 452
Cfr13I GGNCC 1 cut(s) 49
CseI GACGC 1 cut(s) 252
CviAII CATG 9 cut(s) 169, 236, 267, 395, 488, 557, 566, 723, 745
DdeI CTNAG 2 cut(s) 576, 671
DinI GGCGCC 1 cut(s) 451
DpnI GATC 6 cut(s) 29, 110, 157, 332, 519, 610
DpnII GATC 6 cut(s) 27, 108, 155, 330, 517, 608
DraI TTTAAA 1 cut(s) 292
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco130I CCWWGG 1 cut(s) 168
Eco24I GRGCYC 1 cut(s) 75
Eco32I GATATC 1 cut(s) 443
Eco47I GGWCC 1 cut(s) 49
EcoNI CCTNNNNNAGG 1 cut(s) 202
EcoRI GAATTC 1 cut(s) 421
EcoRII CCWGG 3 cut(s) 309, 460, 863
EcoRV GATATC 1 cut(s) 443
EcoT14I CCWWGG 1 cut(s) 168
EcoT38I GRGCYC 1 cut(s) 75
EgeI GGCGCC 1 cut(s) 451
EheI GGCGCC 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 9 cut(s) 172, 239, 270, 398, 491, 560, 569, 726, 748
FatI CATG 9 cut(s) 168, 235, 266, 394, 487, 556, 565, 722, 744
FauI CCCGC 3 cut(s) 82, 461, 783
FblI GTMKAC 2 cut(s) 141, 373
Fnu4HI GCNGC 2 cut(s) 66, 589
FokI GGATG 3 cut(s) 293, 298, 920
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 2 cut(s) 66, 589
FspBI CTAG 2 cut(s) 56, 606
GlaI GCGC 3 cut(s) 64, 148, 451
GluI GCNGC 2 cut(s) 66, 589
GsuI CTGGAG 1 cut(s) 597
HaeII RGCGCY 2 cut(s) 150, 453
HaeIII GGCC 1 cut(s) 367
HapII CCGG 2 cut(s) 52, 121
HgaI GACGC 1 cut(s) 252
HhaI GCGC 3 cut(s) 65, 149, 452
Hin1I GRCGYC 1 cut(s) 450
Hin1II CATG 9 cut(s) 172, 239, 270, 398, 491, 560, 569, 726, 748
Hin6I GCGC 3 cut(s) 63, 147, 450
HinP1I GCGC 3 cut(s) 63, 147, 450
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 3 cut(s) 92, 164, 353
HpaII CCGG 2 cut(s) 52, 121
Hpy166II GTNNAC 3 cut(s) 142, 223, 374
Hpy188I TCNGA 1 cut(s) 672
Hpy188III TCNNGA 6 cut(s) 31, 236, 502, 557, 614, 740
Hpy8I GTNNAC 3 cut(s) 142, 223, 374
Hpy99I CGWCG 3 cut(s) 91, 143, 146
HpyAV CCTTC 2 cut(s) 336, 842
HpyCH4III ACNGT 2 cut(s) 508, 895
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 7 cut(s) 153, 344, 722, 799, 828, 844, 911
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 719, 796, 860
HpyF3I CTNAG 2 cut(s) 576, 671
HpySE526I ACGT 1 cut(s) 299
Hsp92I GRCGYC 1 cut(s) 450
Hsp92II CATG 9 cut(s) 172, 239, 270, 398, 491, 560, 569, 726, 748
HspAI GCGC 3 cut(s) 63, 147, 450
KasI GGCGCC 1 cut(s) 449
Kzo9I GATC 6 cut(s) 27, 108, 155, 330, 517, 608
LguI GCTCTTC 1 cut(s) 260
LmnI GCTCC 3 cut(s) 756, 766, 851
Lsp1109I GCAGC 1 cut(s) 575
LweI GCATC 1 cut(s) 898
MaeI CTAG 2 cut(s) 56, 606
MaeII ACGT 1 cut(s) 299
MaeIII GTNAC 3 cut(s) 490, 525, 882
MalI GATC 6 cut(s) 29, 110, 157, 332, 519, 610
MboI GATC 6 cut(s) 27, 108, 155, 330, 517, 608
MboII GAAGA 3 cut(s) 103, 247, 362
MfeI CAATTG 2 cut(s) 130, 521
MflI RGATCY 2 cut(s) 155, 608
MhlI GDGCHC 4 cut(s) 75, 255, 275, 771
MluCI AATT 7 cut(s) 130, 421, 521, 537, 619, 808, 838
Mly113I GGCGCC 1 cut(s) 450
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 1 cut(s) 744
MnlI CCTC 4 cut(s) 88, 184, 555, 825
MseI TTAA 3 cut(s) 212, 291, 807
MslI CAYNNNNRTG 2 cut(s) 240, 727
MspI CCGG 2 cut(s) 52, 121
MspR9I CCNGG 3 cut(s) 311, 462, 865
MssI GTTTAAAC 1 cut(s) 292
MunI CAATTG 2 cut(s) 130, 521
MvaI CCWGG 3 cut(s) 311, 462, 865
MwoI GCNNNNNNNGC 4 cut(s) 74, 719, 796, 860
NarI GGCGCC 1 cut(s) 450
NcoI CCATGG 1 cut(s) 168
NdeII GATC 6 cut(s) 27, 108, 155, 330, 517, 608
NlaIII CATG 9 cut(s) 172, 239, 270, 398, 491, 560, 569, 726, 748
NlaIV GGNNCC 3 cut(s) 50, 272, 451
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 2 cut(s) 525, 882
PagI TCATGA 2 cut(s) 235, 556
PciSI GCTCTTC 1 cut(s) 260
PfeI GAWTC 2 cut(s) 164, 353
PflFI GACNNNGTC 1 cut(s) 91
PflMI CCANNNNNTGG 1 cut(s) 870
PkrI GCNGC 2 cut(s) 67, 590
PleI GAGTC 1 cut(s) 100
PluTI GGCGCC 1 cut(s) 453
PmeI GTTTAAAC 1 cut(s) 292
PpsI GAGTC 1 cut(s) 100
Psp1406I AACGTT 1 cut(s) 299
Psp6I CCWGG 3 cut(s) 309, 460, 863
PspGI CCWGG 3 cut(s) 309, 460, 863
PspN4I GGNNCC 3 cut(s) 50, 272, 451
PspPI GGNCC 1 cut(s) 49
PsuI RGATCY 2 cut(s) 155, 608
PsyI GACNNNGTC 1 cut(s) 91
RseI CAYNNNNRTG 2 cut(s) 240, 727
SalI GTCGAC 1 cut(s) 140
SapI GCTCTTC 1 cut(s) 260
SaqAI TTAA 3 cut(s) 212, 291, 807
SatI GCNGC 2 cut(s) 66, 589
Sau3AI GATC 6 cut(s) 27, 108, 155, 330, 517, 608
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 3 cut(s) 311, 462, 865
SduI GDGCHC 4 cut(s) 75, 255, 275, 771
SetI ASST 4 cut(s) 195, 302, 590, 761
SfaNI GCATC 1 cut(s) 898
SfcI CTRYAG 1 cut(s) 481
SfoI GGCGCC 1 cut(s) 451
SgrDI CGTCGACG 1 cut(s) 140
SinI GGWCC 1 cut(s) 49
SmiMI CAYNNNNRTG 2 cut(s) 240, 727
Sse9I AATT 7 cut(s) 130, 421, 521, 537, 619, 808, 838
SsiI CCGC 5 cut(s) 66, 75, 248, 454, 790
SspDI GGCGCC 1 cut(s) 449
SspMI CTAG 2 cut(s) 56, 606
StyD4I CCNGG 3 cut(s) 309, 460, 863
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 2 cut(s) 508, 895
TaiI ACGT 1 cut(s) 302
TaqI TCGA 3 cut(s) 141, 329, 439
TasI AATT 7 cut(s) 130, 421, 521, 537, 619, 808, 838
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 2 cut(s) 164, 353
Tru1I TTAA 3 cut(s) 212, 291, 807
Tru9I TTAA 3 cut(s) 212, 291, 807
TscAI CASTG 5 cut(s) 519, 534, 580, 801, 940
TseFI GTSAC 2 cut(s) 525, 882
TseI GCWGC 1 cut(s) 588
Tsp45I GTSAC 2 cut(s) 525, 882
TspDTI ATGAA 3 cut(s) 383, 403, 872
TspGWI ACGGA 3 cut(s) 85, 104, 148
TspRI CASTG 5 cut(s) 519, 534, 580, 801, 940
Tth111I GACNNNGTC 1 cut(s) 91
Van91I CCANNNNNTGG 1 cut(s) 870
VpaK11BI GGWCC 1 cut(s) 49
XagI CCTNNNNNAGG 1 cut(s) 202
XapI RAATTY 2 cut(s) 421, 619
XmiI GTMKAC 2 cut(s) 141, 373
XspI CTAG 2 cut(s) 56, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.