Rh7DG462300

5'-adenylylsulfate reductase-like 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008540
Physical Location & Seq
Forward (+)
65854803 .. 65857460
2658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG462300.1

Sequence Viewer

Length: 951 bp
ATGGGGATTAGGGTTTGGGGATTTGGGATCCTGATATTATTGATATGGGGCCGGCTAACGTGCGCCGCCGAGCCGACGTCTTCTCCGACGGAGTCCGCGGCGGAAGCGATCTTTGGATTCCGAGATTCCTATTGTCCGGCTGACGGCGTTACCAGATCCGTTGGTTCTAATGGTGTTATTGTGGGAGATGAGGTTTCGTTACAAAGGGCATTGAATCTGGTTCACAAGAATAGTCACGAGTATGTGGCTGTGCTCTTTTATGCATCTTGGTGCCCTTTCTCAAGGATGTTTAAGCCAACATTTTCTGTTCTGGCTTCTTTGTATCCTTCCATCCCACATTTTGCATTTGAAGAATCAGCCATCAGGCCAAGTATCCTCTCAAAGTATGGAGTTCATGGCTTTCCTACTCTTTTCATTTTGAATTCTACCATGCGAGTTCGATACCAGGGCGCCCGGACTCCTGGTTCTCTTATTGCTTTCTATAGTGAAGTTACTGGCATCAAGACAGTATCATTGGATCAATTGTCACTGGAAAAGGCCGGACACCCACCAAATCATGAGAAGAATGATAGCAATGAGCAGGAAAGCTGCCCCTTTTCTTGGGCAAGATCTCCAGAGAATTTGCTTCGTCAGGAAACATATTTGGCTCTTGCCTCTGCTTTTGTAATTCTGCGTTTGCTCTACATCTTTTTTCCCACTCTGATTTCATTTGCTCAATCCGCATGGAGAAGGCATATCCGAAACGTAAGATTCGGGAGCTTGTTTGAGCACCCTGTGGCCTATCTAAAGCGTGCAGTGCAGTTGTTTAACTCTCTAAAAGAGCCTTGCAAAAGGAGTAATCTGCAGGAAGGAGCAATGAACGCCCGGGTCTGGGCTTCCAAGTCGCTCGCCACAGTTTCCATCGGGGATGCAAGTACCAGCAGGGGTACTGCAGTGACTGAAACCCGTTGA

Protein Analysis

316

Amino Acids

35.14

Weight (kDa)

9.33

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 77 - 160 6.6e-10 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 80
AccB1I GGYRCC 2 cut(s) 270, 449
AccII CGCG 1 cut(s) 98
AciI CCGC 5 cut(s) 66, 96, 98, 101, 720
AclWI GGATC 4 cut(s) 22, 35, 150, 525
AcsI RAATTY 2 cut(s) 421, 619
AcyI GRCGYC 2 cut(s) 77, 450
AdeI CACNNNGTG 1 cut(s) 775
AfaI GTAC 2 cut(s) 916, 928
AfiI CCNNNNNNNGG 4 cut(s) 143, 600, 870, 871
AgsI TTSAA 3 cut(s) 214, 350, 421
AjnI CCWGG 2 cut(s) 444, 460
AjuI GAANNNNNNNTTGG 2 cut(s) 96, 128
AloI GAACNNNNNNTCC 2 cut(s) 448, 480
AluBI AGCT 2 cut(s) 588, 759
AluI AGCT 2 cut(s) 588, 759
Alw21I GWGCWC 2 cut(s) 255, 771
AlwI GGATC 4 cut(s) 22, 35, 150, 525
AlwNI CAGNNNCTG 1 cut(s) 938
Ama87I CYCGRG 1 cut(s) 864
AoxI GGCC 4 cut(s) 49, 365, 537, 777
ApeKI GCWGC 1 cut(s) 588
ApoI RAATTY 2 cut(s) 421, 619
AspLEI GCGC 2 cut(s) 65, 452
AspS9I GGNCC 1 cut(s) 49
AsuC2I CCSGG 3 cut(s) 454, 865, 866
AvaI CYCGRG 1 cut(s) 864
BaeGI GKGCMC 1 cut(s) 275
BaeI ACNNNNGTAYC 2 cut(s) 918, 951
BamHI GGATCC 1 cut(s) 27
BanI GGYRCC 2 cut(s) 270, 449
BauI CACGAG 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 2 cut(s) 255, 771
BbvI GCAGC 1 cut(s) 575
BccI CCATC 3 cut(s) 338, 368, 908
BceAI ACGGC 1 cut(s) 160
BciT130I CCWGG 2 cut(s) 446, 462
BciVI GTATCC 2 cut(s) 333, 383
BcnI CCSGG 3 cut(s) 454, 865, 866
BfmI CTRYAG 3 cut(s) 481, 842, 930
BfoI RGCGCY 1 cut(s) 453
BfuI GTATCC 2 cut(s) 333, 383
BglII AGATCT 1 cut(s) 608
BisI GCNGC 3 cut(s) 66, 99, 589
BlsI GCNGC 3 cut(s) 67, 100, 590
Bme1390I CCNGG 5 cut(s) 446, 454, 462, 865, 866
BmeT110I CYCGRG 1 cut(s) 864
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 4 cut(s) 29, 50, 272, 451
BmrFI CCNGG 5 cut(s) 446, 454, 462, 865, 866
BmsI GCATC 3 cut(s) 272, 507, 898
BpiI GAAGAC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 597
BpuEI CTTGAG 1 cut(s) 265
BpuMI CCSGG 3 cut(s) 454, 865, 866
BsaHI GRCGYC 2 cut(s) 77, 450
BsaJI CCNNGG 3 cut(s) 96, 445, 864
BsaXI ACNNNNNCTCC 4 cut(s) 67, 97, 177, 207
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 600, 870, 871
Bse118I RCCGGY 1 cut(s) 51
Bse1I ACTGG 2 cut(s) 499, 534
Bse3DI GCAATG 2 cut(s) 580, 861
BseBI CCWGG 2 cut(s) 446, 462
BseDI CCNNGG 3 cut(s) 96, 445, 864
BseGI GGATG 3 cut(s) 291, 330, 913
BseLI CCNNNNNNNGG 4 cut(s) 143, 600, 870, 871
BseMI GCAATG 2 cut(s) 580, 861
BseNI ACTGG 2 cut(s) 499, 534
BseSI GKGCMC 1 cut(s) 275
BseXI GCAGC 1 cut(s) 575
BsgI GTGCAG 2 cut(s) 813, 818
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 4 cut(s) 51, 367, 539, 779
BshNI GGYRCC 2 cut(s) 270, 449
BsiHKAI GWGCWC 2 cut(s) 255, 771
BsiHKCI CYCGRG 1 cut(s) 864
BsiSI CCGG 5 cut(s) 52, 137, 454, 540, 865
BslI CCNNNNNNNGG 4 cut(s) 143, 600, 870, 871
BsnI GGCC 4 cut(s) 51, 367, 539, 779
BsoBI CYCGRG 1 cut(s) 864
Bsp1286I GDGCHC 3 cut(s) 255, 275, 771
Bsp143I GATC 5 cut(s) 27, 108, 155, 517, 608
BspACI CCGC 5 cut(s) 66, 96, 98, 101, 720
BspANI GGCC 4 cut(s) 51, 367, 539, 779
BspFNI CGCG 1 cut(s) 98
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 4 cut(s) 29, 50, 272, 451
BspMAI CTGCAG 2 cut(s) 846, 934
BspPI GGATC 4 cut(s) 22, 35, 150, 525
BspT107I GGYRCC 2 cut(s) 270, 449
BsrDI GCAATG 2 cut(s) 580, 861
BsrFI RCCGGY 1 cut(s) 51
BsrI ACTGG 2 cut(s) 499, 534
BssAI RCCGGY 1 cut(s) 51
BssECI CCNNGG 3 cut(s) 96, 445, 864
BssMI GATC 5 cut(s) 27, 108, 155, 517, 608
BssNI GRCGYC 2 cut(s) 77, 450
BssSI CACGAG 1 cut(s) 236
Bst2BI CACGAG 1 cut(s) 236
Bst2UI CCWGG 2 cut(s) 446, 462
Bst4CI ACNGT 2 cut(s) 508, 895
BstACI GRCGYC 2 cut(s) 77, 450
BstC8I GCNNGC 3 cut(s) 53, 792, 888
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 3 cut(s) 291, 330, 913
BstFNI CGCG 1 cut(s) 98
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 2 cut(s) 65, 452
BstKTI GATC 5 cut(s) 30, 111, 158, 520, 611
BstMBI GATC 5 cut(s) 27, 108, 155, 517, 608
BstMWI GCNNNNNNNGC 4 cut(s) 104, 719, 796, 860
BstNI CCWGG 2 cut(s) 446, 462
BstSCI CCNGG 5 cut(s) 444, 452, 460, 863, 864
BstSFI CTRYAG 3 cut(s) 481, 842, 930
BstSLI GKGCMC 1 cut(s) 275
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 1 cut(s) 575
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 3 cut(s) 27, 155, 608
BstYI RGATCY 3 cut(s) 27, 155, 608
BsuI GTATCC 2 cut(s) 333, 383
BsuRI GGCC 4 cut(s) 51, 367, 539, 779
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 3 cut(s) 291, 330, 913
BtsI GCAGTG 2 cut(s) 801, 939
BtsIMutI CAGTG 3 cut(s) 527, 801, 939
Cac8I GCNNGC 3 cut(s) 53, 792, 888
CaiI CAGNNNCTG 1 cut(s) 938
CciI TCATGA 1 cut(s) 556
CfoI GCGC 2 cut(s) 65, 452
Cfr10I RCCGGY 1 cut(s) 51
Cfr13I GGNCC 1 cut(s) 49
Cfr42I CCGCGG 1 cut(s) 99
Cfr9I CCCGGG 1 cut(s) 864
Csp6I GTAC 2 cut(s) 915, 927
CviAII CATG 4 cut(s) 395, 430, 557, 723
CviQI GTAC 2 cut(s) 915, 927
DinI GGCGCC 1 cut(s) 451
DpnI GATC 5 cut(s) 29, 110, 157, 519, 610
DpnII GATC 5 cut(s) 27, 108, 155, 517, 608
DraIII CACNNNGTG 1 cut(s) 775
EciI GGCGGA 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 864
EcoRI GAATTC 1 cut(s) 421
EcoRII CCWGG 2 cut(s) 444, 460
EcoT22I ATGCAT 1 cut(s) 265
EgeI GGCGCC 1 cut(s) 451
EheI GGCGCC 1 cut(s) 451
FaeI CATG 4 cut(s) 398, 433, 560, 726
FatI CATG 4 cut(s) 394, 429, 556, 722
Fnu4HI GCNGC 3 cut(s) 66, 99, 589
FokI GGATG 3 cut(s) 298, 317, 920
Fsp4HI GCNGC 3 cut(s) 66, 99, 589
GlaI GCGC 2 cut(s) 64, 451
GluI GCNGC 3 cut(s) 66, 99, 589
GsuI CTGGAG 1 cut(s) 597
HaeII RGCGCY 1 cut(s) 453
HaeIII GGCC 4 cut(s) 51, 367, 539, 779
HapII CCGG 5 cut(s) 52, 137, 454, 540, 865
HhaI GCGC 2 cut(s) 65, 452
Hin1I GRCGYC 2 cut(s) 77, 450
Hin1II CATG 4 cut(s) 398, 433, 560, 726
Hin6I GCGC 2 cut(s) 63, 450
HinP1I GCGC 2 cut(s) 63, 450
HinfI GANTC 7 cut(s) 92, 117, 125, 214, 353, 457, 750
HpaII CCGG 5 cut(s) 52, 137, 454, 540, 865
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 4 cut(s) 87, 122, 702, 740
Hpy188III TCNNGA 7 cut(s) 31, 236, 502, 557, 614, 632, 754
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 2 cut(s) 79, 91
HpyAV CCTTC 3 cut(s) 336, 723, 842
HpyCH4III ACNGT 2 cut(s) 508, 895
HpyCH4IV ACGT 3 cut(s) 59, 77, 744
HpyCH4V TGCA 8 cut(s) 263, 344, 794, 799, 828, 844, 911, 932
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 719, 796, 860
HpySE526I ACGT 3 cut(s) 59, 77, 744
Hsp92I GRCGYC 2 cut(s) 77, 450
Hsp92II CATG 4 cut(s) 398, 433, 560, 726
HspAI GCGC 2 cut(s) 63, 450
KasI GGCGCC 1 cut(s) 449
KroI GCCGGC 1 cut(s) 51
KroNI GCCGGC 1 cut(s) 53
KspI CCGCGG 1 cut(s) 99
Kzo9I GATC 5 cut(s) 27, 108, 155, 517, 608
LmnI GCTCC 2 cut(s) 756, 851
Lsp1109I GCAGC 1 cut(s) 575
LweI GCATC 3 cut(s) 272, 507, 898
MaeII ACGT 3 cut(s) 59, 77, 744
MaeIII GTNAC 6 cut(s) 148, 198, 233, 490, 525, 934
MalI GATC 5 cut(s) 29, 110, 157, 519, 610
MboI GATC 5 cut(s) 27, 108, 155, 517, 608
MboII GAAGA 3 cut(s) 72, 362, 574
MfeI CAATTG 1 cut(s) 521
MflI RGATCY 3 cut(s) 27, 155, 608
MhlI GDGCHC 3 cut(s) 255, 275, 771
MluCI AATT 4 cut(s) 421, 521, 619, 666
Mly113I GGCGCC 1 cut(s) 450
MlyI GAGTC 2 cut(s) 101, 451
MmeI TCCRAC 1 cut(s) 110
MnlI CCTC 3 cut(s) 184, 386, 664
Mph1103I ATGCAT 1 cut(s) 265
MroNI GCCGGC 1 cut(s) 51
MseI TTAA 2 cut(s) 291, 807
MslI CAYNNNNRTG 2 cut(s) 240, 268
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 5 cut(s) 52, 137, 454, 540, 865
MspR9I CCNGG 5 cut(s) 446, 454, 462, 865, 866
MunI CAATTG 1 cut(s) 521
MvaI CCWGG 2 cut(s) 446, 462
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 4 cut(s) 104, 719, 796, 860
NaeI GCCGGC 1 cut(s) 53
NarI GGCGCC 1 cut(s) 450
NciI CCSGG 3 cut(s) 454, 865, 866
NdeII GATC 5 cut(s) 27, 108, 155, 517, 608
NgoMIV GCCGGC 1 cut(s) 51
NlaIII CATG 4 cut(s) 398, 433, 560, 726
NlaIV GGNNCC 4 cut(s) 29, 50, 272, 451
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 3 cut(s) 233, 525, 934
NsiI ATGCAT 1 cut(s) 265
PagI TCATGA 1 cut(s) 556
PcsI WCGNNNNNNNCGW 1 cut(s) 83
PdiI GCCGGC 1 cut(s) 53
PfeI GAWTC 5 cut(s) 117, 125, 214, 353, 750
PflFI GACNNNGTC 1 cut(s) 91
PkrI GCNGC 3 cut(s) 67, 100, 590
PleI GAGTC 2 cut(s) 100, 451
PluTI GGCGCC 1 cut(s) 453
PpsI GAGTC 2 cut(s) 100, 451
Psp6I CCWGG 2 cut(s) 444, 460
PspGI CCWGG 2 cut(s) 444, 460
PspN4I GGNNCC 4 cut(s) 29, 50, 272, 451
PspPI GGNCC 1 cut(s) 49
PstI CTGCAG 2 cut(s) 846, 934
PstNI CAGNNNCTG 1 cut(s) 938
PsuI RGATCY 3 cut(s) 27, 155, 608
PsyI GACNNNGTC 1 cut(s) 91
RsaI GTAC 2 cut(s) 916, 928
RsaNI GTAC 2 cut(s) 915, 927
RseI CAYNNNNRTG 2 cut(s) 240, 268
SacII CCGCGG 1 cut(s) 99
SaqAI TTAA 2 cut(s) 291, 807
SatI GCNGC 3 cut(s) 66, 99, 589
Sau3AI GATC 5 cut(s) 27, 108, 155, 517, 608
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 2 cut(s) 101, 451
ScrFI CCNGG 5 cut(s) 446, 454, 462, 865, 866
SduI GDGCHC 3 cut(s) 255, 275, 771
SetI ASST 6 cut(s) 62, 80, 195, 590, 747, 761
SfaNI GCATC 3 cut(s) 272, 507, 898
SfcI CTRYAG 3 cut(s) 481, 842, 930
SfoI GGCGCC 1 cut(s) 451
Sfr303I CCGCGG 1 cut(s) 99
SgrBI CCGCGG 1 cut(s) 99
SmaI CCCGGG 1 cut(s) 866
SmiMI CAYNNNNRTG 2 cut(s) 240, 268
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
Sse9I AATT 4 cut(s) 421, 521, 619, 666
SsiI CCGC 5 cut(s) 66, 96, 98, 101, 720
SspDI GGCGCC 1 cut(s) 449
StyD4I CCNGG 5 cut(s) 444, 452, 460, 863, 864
TaaI ACNGT 2 cut(s) 508, 895
TaiI ACGT 3 cut(s) 62, 80, 747
TaqI TCGA 1 cut(s) 439
TasI AATT 4 cut(s) 421, 521, 619, 666
TauI GCSGC 2 cut(s) 68, 101
TfiI GAWTC 5 cut(s) 117, 125, 214, 353, 750
Tru1I TTAA 2 cut(s) 291, 807
Tru9I TTAA 2 cut(s) 291, 807
TscAI CASTG 3 cut(s) 534, 801, 939
TseFI GTSAC 3 cut(s) 233, 525, 934
TseI GCWGC 1 cut(s) 588
Tsp45I GTSAC 3 cut(s) 233, 525, 934
TspDTI ATGAA 4 cut(s) 383, 403, 696, 872
TspGWI ACGGA 2 cut(s) 104, 148
TspMI CCCGGG 1 cut(s) 864
TspRI CASTG 3 cut(s) 534, 801, 939
Tth111I GACNNNGTC 1 cut(s) 91
XapI RAATTY 2 cut(s) 421, 619
XmaI CCCGGG 1 cut(s) 864
ZraI GACGTC 1 cut(s) 78
Zsp2I ATGCAT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.