FvH4_5g39170

Glutamyl endopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002809
Physical Location & Seq
Reverse (-)
28909317 .. 28915032
5716 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g39170.t2

Sequence Viewer

Length: 2727 bp
ATGCTCCATCTTCAAAAAGTCTATCATCGCCTCTCTCTTCGCTCCTTCACTCCTCACTCCCTCCCTTTCCAATTCCCATCCCTCCTCTCCCCCACGCGCCCCCGCCGTAGCCACGCCGTCAGGTCCATCCGAACCTCCGCCGCCATGTCTGCTACCTCCAGGCTCAGCAACATCGTCCCCCTCAACGCCGTCGTCGCCGAGAACGCCGGCAACGGTTCCGTCTCTTCTGCCACTGCTACTACTTCATTCGAAGATGAAGATTCCACGATGGGATATCGTCTTCCTCCGAAAGAAATCAAAGATATAGTTGATGCTCCGCCGCTTCCGGCCTTGTCATTCTCACCGCACAGAGACAAGATACTGTTTCTAAAACGGAGAGCTTTGCCCCCGTTGAGTGAGCTCGCGAGACCGGAGGAAAAGCTTGCCGGTGTTCGGATTGATGGAAAATGCAATACAAGGACTCGAATGTCATTTTATACTGGGATTGGGATCCATCAGTTGCTACCGGATGGGACTCTTGGTTCTGAAGTTGAGGTGCATGGATTCCCTGAGGGTGCTAAGATCAATTTTGTTACTTGGTCCCCGGATGGCCGCCATTTGGCCTTCACTATCCGGTTTGATGATGAGAGTACTAGTAGTAAGCTTAAAGTGTGGGTTGCGAAAGTGGAATCCGGGGTAGCCAGGCCGTTGTTGGAGTTGCATGATTACTGTTTGAATGCGGTGTTTGACAATTTTGTTTGGGTGAATGAGTCTTCTTTGTTAGTTTGCACCATTCCTCTGTCTCGTGGAGACCCACCAAAGAAACCTTTGGTGCCTTTTGGGCCTAAAATCCAGTCGAACGAGCAGAAAAACATTATTCAAGTTAGGACATTCCAGGATTTGCTGAAGGATGAGTATGATGAAGATTTATTTGATTACTATGCTACCTCACAGCTTGTTTTAGCTTCTTTGGATGGCACCATCAAGGAAATTGGTCCACCGGCTGTATATACATCAATGGACCCCTCCCCAGATCACAAATATCTTTTGATTAGTTCGCTTCACAGACCATACTCTTTCATTGTACCATGCGGAAGATTTCCCAAGAAGGTTGACATGTGGACAGCTGATGGAAAATTTGTTAGAGAACTTTGTGATTTGCCTCTTGCCGAGGACATCCCCATTGCATTCAATAGTGTGCGAAGAGGGATGCGCTCTCTCAACTGGAGAGCGGATGAACCATCAACACTCTACTGGGTTGAGACCCAAGATGAAGGAGATGCGAAAGTGGAAGTTTCTCCACGTGATATTGTTTATACACAGCCTGCTGAACCACTAGAAGGCAAAGCTCCAACAATCTTACACAAACTCGATCTCCGATATGGGTCATCGGAAGATGTATACTCTGATCCTGGCTCACCCATGCTGCGGAGAACTCCTGCCGGAACGTATGTGCTTGCCAAAGTGAAGAAAGAAAATGATGAAGGCACTTATTTACTACTGAATGGAAATGGTGCTACACCAGAGGGAAATATCCCATTCCTTGATTTGTTTGACATAAATACAGGAAATAAAGAACGAATATGGAAGAGTGACAAAGAAAAGTATTATGAGGGTGTTGTTGCTTTAATGTCTGATGAGAAAGAAGGAGATCTCCCCATCAATACCTTGAAAATATTGACATCCAAAGAGTCAAAAACTGAAAACACTCAATATTATATCCTAAGCTGGCCAGAAAAGAAAGCATGTCAAATTACAAATTTCCCTCATCCGTATCCACAGCTGGCATCATTGCAGAAAGAGATGGTTAGGTACCAGAGAAAGGATGGGGTTCAACTAACCGCAACACTATATCTTCCACCAGGCTACGATCCATCCAGGGATGGCCCTCTTCCATGTTTGTTTTGGTCTTACCCTGGAGAATTCAAAAGCAAAGATGCTGCCGGACAAGTTCGTGGTTCTCCTAATGAATTTGCTGGGATAGGTCCAACATCAGCCCTGCTCTGGATGGCTCGAAGGTTTGCAATTTTATCTGGACCAACTATTCCTATTATTGGTGAGGGTGATGAGGAAGCAAATGATAGGTATGTGGAGCAATTAGTTGCAAGTGCCGAGGCTGCTGTGGAGGAAGTCATTAGACGTGGAGTGGCTCATCCAAAGAAAATTGCTGTTGGGGGACATTCATATGGGGCGTTCATGACTGCAAACCTACTGGCACATGCTCCTCATCTTTTTGCTTGTGGAGTTGCTCGTTCGGGTGCTTACAACAGAACACTTACTCCTTTCGGTTTTCAGAACGAGGAAAGAACTCTCTGGGAGGCTACTAGTACTTATGTGGAGATGAGTCCATTCATGTCAGCTAATAAAATAAAGAAGCCAATCTTGCTTATCCATGGAGAGGAAGACAATAACCCAGGGACTTTAACCATGCAGTCAGATCGTTTCTTTAATGCTTTGAAAGGTCATGGTGCTCTTTGCCGCCTTGTGATTCTTCCTTTTGAGAGCCATGGCTATGCTGCAAGAGAAAGTATCATGCATGTATTATGGGAAACTGACAGGTGGTTGCAAAAATATTGCGTGTCTGACACTTCTGATGTTAATGTTGATGAAGATGCATGTAAAGACAATGTAGGCACAGGATCTACAAATTCTGAAAACAAAACAGTGGCCACTGGTGGAGGCAGTGCCTCAGAAGTGTCAAGTTCTGAGCATGAAGGAGTTGATTCTTTGCCGAGGTCATTGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

909

Amino Acids

100.84

Weight (kDa)

5.89

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 744 - 843 6.5e-16 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 466
Acc65I GGTACC 1 cut(s) 1791
AccB1I GGYRCC 3 cut(s) 811, 956, 1791
AccBSI CCGCTC 1 cut(s) 1211
AccI GTMKAC 1 cut(s) 1380
AccII CGCG 2 cut(s) 97, 404
AclWI GGATC 5 cut(s) 484, 497, 1382, 1844, 2626
AcoI YGGCCR 3 cut(s) 589, 1709, 2646
AcsI RAATTY 5 cut(s) 1115, 1738, 1901, 1949, 2626
AcuI CTGAAG 2 cut(s) 546, 905
AcvI CACGTG 1 cut(s) 1283
AfaI GTAC 4 cut(s) 631, 1065, 1793, 2308
AfiI CCNNNNNNNGG 8 cut(s) 432, 598, 1319, 1407, 1801, 1983, 2033, 2377
AflIII ACRYGT 1 cut(s) 1095
AgsI TTSAA 8 cut(s) 14, 715, 860, 1171, 1651, 1814, 1906, 2437
AhlI ACTAGT 2 cut(s) 632, 2303
AjiI CACGTC 1 cut(s) 2120
AjnI CCWGG 8 cut(s) 158, 680, 873, 1390, 1840, 1856, 1894, 2392
AloI GAACNNNNNNTCC 4 cut(s) 505, 537, 2242, 2274
Alw21I GWGCWC 2 cut(s) 402, 2452
Alw26I GTCTC 6 cut(s) 226, 345, 400, 783, 786, 1235
AlwI GGATC 5 cut(s) 484, 497, 1382, 1844, 2626
AoxI GGCC 8 cut(s) 327, 589, 600, 683, 821, 1709, 1864, 2646
ApeKI GCWGC 4 cut(s) 1405, 1919, 2096, 2495
ApoI RAATTY 5 cut(s) 1115, 1738, 1901, 1949, 2626
Asp718I GGTACC 1 cut(s) 1791
AspLEI GCGC 2 cut(s) 99, 1194
AspS9I GGNCC 8 cut(s) 123, 579, 821, 974, 1000, 1865, 1964, 2015
AsuC2I CCSGG 2 cut(s) 584, 673
AsuHPI GGTGA 5 cut(s) 333, 754, 1389, 2048, 2054
AsuII TTCGAA 1 cut(s) 249
AvaII GGWCC 6 cut(s) 123, 579, 974, 1000, 1964, 2015
AxyI CCTNAGG 1 cut(s) 549
BalI TGGCCA 2 cut(s) 1711, 2648
BamHI GGATCC 1 cut(s) 489
BanI GGYRCC 3 cut(s) 811, 956, 1791
BanII GRGCYC 1 cut(s) 402
BauI CACGAG 1 cut(s) 783
BbrPI CACGTG 1 cut(s) 1283
BbsI GAAGAC 3 cut(s) 272, 744, 2388
Bbv12I GWGCWC 2 cut(s) 402, 2452
BbvI GCAGC 4 cut(s) 1392, 1906, 2083, 2482
BceAI ACGGC 4 cut(s) 90, 101, 173, 670
BcgI CGANNNNNNTGC 2 cut(s) 2399, 2433
BciT130I CCWGG 8 cut(s) 160, 682, 875, 1392, 1842, 1858, 1896, 2394
BciVI GTATCC 1 cut(s) 1764
BcnI CCSGG 2 cut(s) 584, 673
BcoDI GTCTC 6 cut(s) 226, 345, 400, 783, 786, 1235
BcuI ACTAGT 2 cut(s) 632, 2303
BfaI CTAG 3 cut(s) 633, 1316, 2304
BfuI GTATCC 1 cut(s) 1764
BglI GCCNNNNNGGC 1 cut(s) 820
BglII AGATCT 1 cut(s) 1630
BisI GCNGC 8 cut(s) 141, 320, 592, 1406, 1920, 2097, 2458, 2496
BlpI GCTNAGC 1 cut(s) 164
BlsI GCNGC 8 cut(s) 142, 321, 593, 1407, 1921, 2098, 2459, 2497
BmcAI AGTACT 2 cut(s) 631, 2308
Bme18I GGWCC 6 cut(s) 123, 579, 974, 1000, 1964, 2015
BmgBI CACGTC 1 cut(s) 2120
BmgT120I GGNCC 8 cut(s) 123, 579, 821, 974, 1000, 1865, 1964, 2015
BmiI GGNNCC 7 cut(s) 217, 491, 581, 813, 958, 1002, 1793
BmrI ACTGGG 2 cut(s) 489, 1243
BmsI GCATC 6 cut(s) 301, 1179, 1249, 1775, 1906, 2581
BmuI ACTGGG 2 cut(s) 489, 1243
BpiI GAAGAC 3 cut(s) 272, 744, 2388
BpmI CTGGAG 3 cut(s) 142, 1225, 1917
Bpu10I CCTNAGC 1 cut(s) 1703
Bpu1102I GCTNAGC 1 cut(s) 164
Bpu14I TTCGAA 1 cut(s) 249
BpuMI CCSGG 2 cut(s) 584, 673
BsaAI YACGTR 1 cut(s) 1283
BsaBI GATNNNNATC 1 cut(s) 488
BsaI GGTCTC 3 cut(s) 400, 783, 1235
BsaWI WCCGGW 3 cut(s) 409, 505, 612
BsaXI ACNNNNNCTCC 4 cut(s) 1338, 1368, 2242, 2272
Bsc4I CCNNNNNNNGG 8 cut(s) 432, 598, 1319, 1407, 1801, 1983, 2033, 2377
Bse118I RCCGGY 3 cut(s) 206, 425, 979
Bse1I ACTGG 6 cut(s) 484, 832, 1208, 1238, 2196, 2656
Bse21I CCTNAGG 1 cut(s) 549
Bse3DI GCAATG 2 cut(s) 1161, 1769
Bse8I GATNNNNATC 1 cut(s) 488
BseBI CCWGG 8 cut(s) 160, 682, 875, 1392, 1842, 1858, 1896, 2394
BseJI GATNNNNATC 1 cut(s) 488
BseLI CCNNNNNNNGG 8 cut(s) 432, 598, 1319, 1407, 1801, 1983, 2033, 2377
BseMI GCAATG 2 cut(s) 1161, 1769
BseMII CTCAG 4 cut(s) 178, 540, 2676, 2682
BseNI ACTGG 6 cut(s) 484, 832, 1208, 1238, 2196, 2656
BseRI GAGGAG 3 cut(s) 42, 74, 2193
BseXI GCAGC 4 cut(s) 1392, 1906, 2083, 2482
BseYI CCCAGC 1 cut(s) 1955
Bsh1236I CGCG 2 cut(s) 97, 404
BshFI GGCC 8 cut(s) 329, 591, 602, 685, 823, 1711, 1866, 2648
BshNI GGYRCC 3 cut(s) 811, 956, 1791
BsiHKAI GWGCWC 2 cut(s) 402, 2452
BslFI GGGAC 5 cut(s) 161, 526, 565, 2169, 2410
BslI CCNNNNNNNGG 8 cut(s) 432, 598, 1319, 1407, 1801, 1983, 2033, 2377
BsmAI GTCTC 6 cut(s) 226, 345, 400, 783, 786, 1235
BsmBI CGTCTC 1 cut(s) 226
BsmFI GGGAC 5 cut(s) 161, 526, 565, 2169, 2410
BsmI GAATGC 2 cut(s) 721, 1166
BsnI GGCC 8 cut(s) 329, 591, 602, 685, 823, 1711, 1866, 2648
Bso31I GGTCTC 3 cut(s) 400, 783, 1235
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 2 cut(s) 402, 2452
Bsp143I GATC 9 cut(s) 489, 561, 1012, 1351, 1387, 1630, 1849, 2416, 2618
Bsp1720I GCTNAGC 1 cut(s) 164
Bsp19I CCATGG 2 cut(s) 2371, 2485
Bsp68I TCGCGA 1 cut(s) 404
BspANI GGCC 8 cut(s) 329, 591, 602, 685, 823, 1711, 1866, 2648
BspCNI CTCAG 4 cut(s) 177, 541, 2677, 2681
BspFNI CGCG 2 cut(s) 97, 404
BspHI TCATGA 1 cut(s) 2175
BspLI GGNNCC 7 cut(s) 217, 491, 581, 813, 958, 1002, 1793
BspPI GGATC 5 cut(s) 484, 497, 1382, 1844, 2626
BspT104I TTCGAA 1 cut(s) 249
BspT107I GGYRCC 3 cut(s) 811, 956, 1791
BspTNI GGTCTC 3 cut(s) 400, 783, 1235
BsrBI CCGCTC 1 cut(s) 1211
BsrDI GCAATG 2 cut(s) 1161, 1769
BsrFI RCCGGY 3 cut(s) 206, 425, 979
BsrI ACTGG 6 cut(s) 484, 832, 1208, 1238, 2196, 2656
BssAI RCCGGY 3 cut(s) 206, 425, 979
BssMI GATC 9 cut(s) 489, 561, 1012, 1351, 1387, 1630, 1849, 2416, 2618
BssNAI GTATAC 1 cut(s) 1381
BssSI CACGAG 1 cut(s) 783
BssT1I CCWWGG 2 cut(s) 2371, 2485
Bst1107I GTATAC 1 cut(s) 1381
Bst2BI CACGAG 1 cut(s) 783
Bst2UI CCWGG 8 cut(s) 160, 682, 875, 1392, 1842, 1858, 1896, 2394
Bst4CI ACNGT 4 cut(s) 215, 363, 710, 2644
Bst6I CTCTTC 5 cut(s) 42, 229, 1177, 1562, 1875
BstBAI YACGTR 1 cut(s) 1283
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 7 cut(s) 208, 402, 423, 1305, 1437, 1709, 1764
BstDEI CTNAG 6 cut(s) 164, 549, 558, 1703, 2668, 2685
BstDSI CCRYGG 2 cut(s) 2371, 2485
BstFNI CGCG 2 cut(s) 97, 404
BstHHI GCGC 2 cut(s) 99, 1194
BstKTI GATC 9 cut(s) 492, 564, 1015, 1354, 1390, 1633, 1852, 2419, 2621
BstMAI GTCTC 6 cut(s) 226, 345, 400, 783, 786, 1235
BstMBI GATC 9 cut(s) 489, 561, 1012, 1351, 1387, 1630, 1849, 2416, 2618
BstMWI GCNNNNNNNGC 6 cut(s) 149, 194, 203, 820, 2096, 2362
BstNI CCWGG 8 cut(s) 160, 682, 875, 1392, 1842, 1858, 1896, 2394
BstNSI RCATGY 5 cut(s) 1099, 1728, 2201, 2519, 2598
BstUI CGCG 2 cut(s) 97, 404
BstV1I GCAGC 4 cut(s) 1392, 1906, 2083, 2482
BstV2I GAAGAC 3 cut(s) 272, 744, 2388
BstX2I RGATCY 3 cut(s) 489, 1630, 2618
BstYI RGATCY 3 cut(s) 489, 1630, 2618
BstZ17I GTATAC 1 cut(s) 1381
Bsu36I CCTNAGG 1 cut(s) 549
BsuI GTATCC 1 cut(s) 1764
BsuRI GGCC 8 cut(s) 329, 591, 602, 685, 823, 1711, 1866, 2648
BtgI CCRYGG 2 cut(s) 2371, 2485
BtgZI GCGATG 1 cut(s) 11
BtrI CACGTC 1 cut(s) 2120
BtsI GCAGTG 2 cut(s) 231, 2668
BtsIMutI CAGTG 4 cut(s) 231, 2649, 2649, 2668
BtuMI TCGCGA 1 cut(s) 404
Cac8I GCNNGC 7 cut(s) 208, 402, 423, 1305, 1437, 1709, 1764
CciI TCATGA 1 cut(s) 2175
CfoI GCGC 2 cut(s) 99, 1194
Cfr10I RCCGGY 3 cut(s) 206, 425, 979
Cfr13I GGNCC 8 cut(s) 123, 579, 821, 974, 1000, 1865, 1964, 2015
Csp6I GTAC 4 cut(s) 630, 1064, 1792, 2307
CviQI GTAC 4 cut(s) 630, 1064, 1792, 2307
DdeI CTNAG 6 cut(s) 164, 549, 558, 1703, 2668, 2685
DpnI GATC 9 cut(s) 491, 563, 1014, 1353, 1389, 1632, 1851, 2418, 2620
DpnII GATC 9 cut(s) 489, 561, 1012, 1351, 1387, 1630, 1849, 2416, 2618
DrdI GACNNNNNNGTC 1 cut(s) 466
DseDI GACNNNNNNGTC 1 cut(s) 466
EaeI YGGCCR 3 cut(s) 589, 1709, 2646
Eam1104I CTCTTC 5 cut(s) 42, 229, 1177, 1562, 1875
EarI CTCTTC 5 cut(s) 42, 229, 1177, 1562, 1875
EciI GGCGGA 2 cut(s) 127, 306
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 2 cut(s) 2371, 2485
Eco24I GRGCYC 1 cut(s) 402
Eco31I GGTCTC 3 cut(s) 400, 783, 1235
Eco32I GATATC 1 cut(s) 275
Eco47I GGWCC 6 cut(s) 123, 579, 974, 1000, 1964, 2015
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 2 cut(s) 546, 905
Eco72I CACGTG 1 cut(s) 1283
Eco81I CCTNAGG 1 cut(s) 549
EcoICRI GAGCTC 1 cut(s) 400
EcoRI GAATTC 1 cut(s) 1901
EcoRII CCWGG 8 cut(s) 158, 680, 873, 1390, 1840, 1856, 1894, 2392
EcoRV GATATC 1 cut(s) 275
EcoT14I CCWWGG 2 cut(s) 2371, 2485
EcoT22I ATGCAT 2 cut(s) 2517, 2596
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 2 cut(s) 2371, 2485
Esp3I CGTCTC 1 cut(s) 226
FalI AAGNNNNNCTT 2 cut(s) 2345, 2377
FaqI GGGAC 5 cut(s) 161, 526, 565, 2169, 2410
FauI CCCGC 1 cut(s) 110
FauNDI CATATG 1 cut(s) 2164
FblI GTMKAC 1 cut(s) 1380
Fnu4HI GCNGC 8 cut(s) 141, 320, 592, 1406, 1920, 2097, 2458, 2496
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 8 cut(s) 141, 320, 592, 1406, 1920, 2097, 2458, 2496
FspBI CTAG 3 cut(s) 633, 1316, 2304
GlaI GCGC 2 cut(s) 98, 1193
GluI GCNGC 8 cut(s) 141, 320, 592, 1406, 1920, 2097, 2458, 2496
GsaI CCCAGC 1 cut(s) 1959
GsuI CTGGAG 3 cut(s) 142, 1225, 1917
HaeIII GGCC 8 cut(s) 329, 591, 602, 685, 823, 1711, 1866, 2648
HhaI GCGC 2 cut(s) 99, 1194
Hin6I GCGC 2 cut(s) 97, 1192
HinP1I GCGC 2 cut(s) 97, 1192
HincII GTYRAC 1 cut(s) 1093
HindII GTYRAC 1 cut(s) 1093
HindIII AAGCTT 2 cut(s) 419, 641
HphI GGTGA 5 cut(s) 333, 754, 1389, 2048, 2054
Hpy166II GTNNAC 4 cut(s) 977, 1093, 1101, 1381
Hpy188III TCNNGA 4 cut(s) 403, 1984, 2013, 2176
Hpy8I GTNNAC 4 cut(s) 977, 1093, 1101, 1381
Hpy99I CGWCG 2 cut(s) 194, 197
HpyCH4III ACNGT 4 cut(s) 215, 363, 710, 2644
HpyCH4IV ACGT 3 cut(s) 1282, 1427, 2119
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 194, 203, 820, 2096, 2362
HpyF3I CTNAG 6 cut(s) 164, 549, 558, 1703, 2668, 2685
HpySE526I ACGT 3 cut(s) 1282, 1427, 2119
HspAI GCGC 2 cut(s) 97, 1192
KpnI GGTACC 1 cut(s) 1795
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 9 cut(s) 489, 561, 1012, 1351, 1387, 1630, 1849, 2416, 2618
LmnI GCTCC 6 cut(s) 9, 47, 319, 1333, 2071, 2206
Lsp1109I GCAGC 4 cut(s) 1392, 1906, 2083, 2482
LweI GCATC 6 cut(s) 301, 1179, 1249, 1775, 1906, 2581
MaeI CTAG 3 cut(s) 633, 1316, 2304
MaeII ACGT 3 cut(s) 1282, 1427, 2119
MaeIII GTNAC 2 cut(s) 571, 1571
MalI GATC 9 cut(s) 491, 563, 1014, 1353, 1389, 1632, 1851, 2418, 2620
MbiI CCGCTC 1 cut(s) 1211
MboI GATC 9 cut(s) 489, 561, 1012, 1351, 1387, 1630, 1849, 2416, 2618
MflI RGATCY 3 cut(s) 489, 1630, 2618
MhlI GDGCHC 2 cut(s) 402, 2452
MlsI TGGCCA 2 cut(s) 1711, 2648
MluNI TGGCCA 2 cut(s) 1711, 2648
MlyI GAGTC 5 cut(s) 454, 508, 758, 1679, 2332
MmeI TCCRAC 3 cut(s) 672, 1355, 1991
Mox20I TGGCCA 2 cut(s) 1711, 2648
Mph1103I ATGCAT 2 cut(s) 2517, 2596
MroNI GCCGGC 1 cut(s) 206
MscI TGGCCA 2 cut(s) 1711, 2648
MseI TTAA 5 cut(s) 645, 1607, 2402, 2427, 2577
MslI CAYNNNNRTG 3 cut(s) 2163, 2490, 2722
Msp20I TGGCCA 2 cut(s) 1711, 2648
MspA1I CMGCKG 2 cut(s) 1106, 1762
Mva1269I GAATGC 2 cut(s) 721, 1166
MvaI CCWGG 8 cut(s) 160, 682, 875, 1392, 1842, 1858, 1896, 2394
MvnI CGCG 2 cut(s) 97, 404
MwoI GCNNNNNNNGC 6 cut(s) 149, 194, 203, 820, 2096, 2362
NaeI GCCGGC 1 cut(s) 208
NciI CCSGG 2 cut(s) 584, 673
NcoI CCATGG 2 cut(s) 2371, 2485
NdeI CATATG 1 cut(s) 2164
NdeII GATC 9 cut(s) 489, 561, 1012, 1351, 1387, 1630, 1849, 2416, 2618
NgoMIV GCCGGC 1 cut(s) 206
NlaIV GGNNCC 7 cut(s) 217, 491, 581, 813, 958, 1002, 1793
NmeAIII GCCGAG 3 cut(s) 223, 1174, 2116
NmuCI GTSAC 1 cut(s) 1571
NruI TCGCGA 1 cut(s) 404
NsiI ATGCAT 2 cut(s) 2517, 2596
NspI RCATGY 5 cut(s) 1099, 1728, 2201, 2519, 2598
NspV TTCGAA 1 cut(s) 249
PagI TCATGA 1 cut(s) 2175
PasI CCCWGGG 1 cut(s) 2393
PciI ACATGT 1 cut(s) 1095
PctI GAATGC 2 cut(s) 721, 1166
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 5 cut(s) 260, 543, 668, 2467, 2702
PfoI TCCNGGA 1 cut(s) 873
PkrI GCNGC 8 cut(s) 142, 321, 593, 1407, 1921, 2098, 2459, 2497
PleI GAGTC 5 cut(s) 454, 508, 757, 1678, 2331
PmaCI CACGTG 1 cut(s) 1283
PmlI CACGTG 1 cut(s) 1283
PpsI GAGTC 5 cut(s) 454, 508, 757, 1678, 2331
Ppu21I YACGTR 1 cut(s) 1283
PscI ACATGT 1 cut(s) 1095
Psp124BI GAGCTC 1 cut(s) 402
Psp6I CCWGG 8 cut(s) 158, 680, 873, 1390, 1840, 1856, 1894, 2392
PspCI CACGTG 1 cut(s) 1283
PspFI CCCAGC 1 cut(s) 1955
PspGI CCWGG 8 cut(s) 158, 680, 873, 1390, 1840, 1856, 1894, 2392
PspN4I GGNNCC 7 cut(s) 217, 491, 581, 813, 958, 1002, 1793
PspPI GGNCC 8 cut(s) 123, 579, 821, 974, 1000, 1865, 1964, 2015
PsuI RGATCY 3 cut(s) 489, 1630, 2618
PvuII CAGCTG 2 cut(s) 1106, 1762
RruI TCGCGA 1 cut(s) 404
RsaI GTAC 4 cut(s) 631, 1065, 1793, 2308
RsaNI GTAC 4 cut(s) 630, 1064, 1792, 2307
RseI CAYNNNNRTG 3 cut(s) 2163, 2490, 2722
SacI GAGCTC 1 cut(s) 402
SaqAI TTAA 5 cut(s) 645, 1607, 2402, 2427, 2577
SatI GCNGC 8 cut(s) 141, 320, 592, 1406, 1920, 2097, 2458, 2496
Sau3AI GATC 9 cut(s) 489, 561, 1012, 1351, 1387, 1630, 1849, 2416, 2618
Sau96I GGNCC 8 cut(s) 123, 579, 821, 974, 1000, 1865, 1964, 2015
ScaI AGTACT 2 cut(s) 631, 2308
SchI GAGTC 5 cut(s) 454, 508, 758, 1679, 2332
SduI GDGCHC 2 cut(s) 402, 2452
SfaNI GCATC 6 cut(s) 301, 1179, 1249, 1775, 1906, 2581
SfuI TTCGAA 1 cut(s) 249
SinI GGWCC 6 cut(s) 123, 579, 974, 1000, 1964, 2015
SmiMI CAYNNNNRTG 3 cut(s) 2163, 2490, 2722
SpeI ACTAGT 2 cut(s) 632, 2303
SspI AATATT 3 cut(s) 1656, 1694, 2552
SspMI CTAG 3 cut(s) 633, 1316, 2304
SstI GAGCTC 1 cut(s) 402
StyI CCWWGG 2 cut(s) 2371, 2485
TaaI ACNGT 4 cut(s) 215, 363, 710, 2644
TaiI ACGT 3 cut(s) 1285, 1430, 2122
TaqI TCGA 5 cut(s) 249, 463, 836, 1350, 1993
TatI WGTACW 2 cut(s) 629, 2306
TauI GCSGC 4 cut(s) 143, 322, 594, 2460
TfiI GAWTC 5 cut(s) 260, 543, 668, 2467, 2702
Tru1I TTAA 5 cut(s) 645, 1607, 2402, 2427, 2577
Tru9I TTAA 5 cut(s) 645, 1607, 2402, 2427, 2577
TscAI CASTG 4 cut(s) 238, 2649, 2656, 2668
TseFI GTSAC 1 cut(s) 1571
TseI GCWGC 4 cut(s) 1405, 1919, 2096, 2495
Tsp45I GTSAC 1 cut(s) 1571
TspGWI ACGGA 3 cut(s) 208, 388, 1740
TspRI CASTG 4 cut(s) 238, 2649, 2656, 2668
VpaK11BI GGWCC 6 cut(s) 123, 579, 974, 1000, 1964, 2015
XapI RAATTY 5 cut(s) 1115, 1738, 1901, 1949, 2626
XceI RCATGY 5 cut(s) 1099, 1728, 2201, 2519, 2598
XcmI CCANNNNNNNNNTGG 3 cut(s) 688, 1802, 1881
XmiI GTMKAC 1 cut(s) 1380
XspI CTAG 3 cut(s) 633, 1316, 2304
ZrmI AGTACT 2 cut(s) 631, 2308
Zsp2I ATGCAT 2 cut(s) 2517, 2596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.