Rroxscaffold_3G00219150

Glutamyl endopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002809
Physical Location & Seq
Forward (+)
1313113 .. 1318718
5606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00219150.1

Sequence Viewer

Length: 2895 bp
ATGAGCGATAGGATGCTTCATCTTCAAAAAGTCTATCATCGCCTATCTCTTCGCTCCTTCACTCCACACTCCCTCCCTTTCAAACCCCCCCAATTCCCATCCCTCCGTTCGCCTCCGCGCCTCCGTGTGCGGCCGCGAAGCCACGCCGTCAGGTCCATCGGAACCGCCGCCGCCGTCATGTCTACTAGCTCTAGCTCTAGGCTCAGCAACATCGTCCCCCTCAACGCCGTGGTTGCCGAGGACGGCGGCAACGGTTCCGTCTCTTCTGCCACTGCTACTACTCCATTTGAAGATGAAGAAGATTCCACGTTGGGATATCGTCTTCCTCCAAAAGAAATTAAAGATATAGTTGATGCTCCGCCGCTTCCGGCCTTGTCATTCTCGCCGCACAGGGATAAAATACTGTTTCTGAAGCGGAGAGCTTTGCCGCCGTTGACAGAACTTGCAAGACCAGAGGAGAAGCTTGCTGGTGTTCGGATTGATGGGAAATGCAATACAAGGACTCGGATGTCGTTTTATACTGGGGTTGGGATCCATCAATTGTTGCCGGATGGTACTCTTGGTTCCGAAGTTGAGGTACATGGGTTCCCTGAGGGTGCTAAGATCAATTTTGTTACTTGGTCCCCAGATGGTCGCCATTTGGCCTTCACTATTCGATTTGATGATGAAGATACTAGTAGTAAGCTTAAAGTGTGGGTTGCCCAAGTAGAAACTGGGGTAGCCAGGCCTTTGTTTGAGTCACATGAATACTGTTTGAATGCGGTTTTTGACAATTTTGTTTGGGTGAACGACTCTTCTTTATTAGTTTGCACCATTCCTCTGTCCCGTGGAGAGCCGCCAAAGAAACCTTTGGTGCCTTTTGGGCCTAAAATTCAATCTAACGAGCAGAAAAACATTATTCAAGTCAGGACCTTCCAGGATTTGCTGAAGGATGAATATGATGAAGATTTGTTTGATTACTATGCCACCTCACAGCTTGTTTTAGGTTCTTTGGATGGCAGGGTGAAGGAAATTGGTCCAGCGGCTGTATATACATCAATGGACCCCTCCCCAGATCACAAATACCTTTTAATTAGTTCCCTTCACAGACCATACTCTTTCATTGTACCATGCGGAAGATTTCCCAAGAAGGTTGAGATGTGGACAGCTGATGGGAAGTTTGTTAGAGAACTTTGTGATTTGCCTCTTGCCGAGGACATCCCCATTGCATTCAATAGTGTGCGAAGAGGGATGCGCTCTCTCAACTGGAGAGCGGATAAATCATCAACACTCTACTGGGTTGAGACCCAAGATGAAGGAGATGCGAAAGTGGAAGTTTCTCCACGTGATATTGTTTATACACAGCCTGCTGAACCACTAGAAGGTGAAGATCCAACAATCTTACACAAACTTGATCTCCGTTATGGAGGGATTTCTTGGTCCGATGATTCACTAGCTCTAGTTTACGAGTCATGGTACAAAACACGGAGAACAAGAACCTGGGTAATTTCTCCTGGATCAAATGATGTCAGTCCCCGCATCCTGTTTGATAGGTCATCGGAAGATGTGTATTCAGATCCTGGCTCCCCCATGCTGCGGAGAACTCCTGCTGGCACATATGTGCTTGCAAAAGTGAAGAAAGAAAATGATGAAGGCACTTATTTACTTTTGAATGGAAATGGTGCTACCCCAGAGGGGAATATCCCTTTCCTCGATTTGTTTGACATAAATACAGGAAATAATGAACGAATATGGAAGAGTGACAAAGAAAAGTATTATGAGGGTGTTGTTGCTTTAATGTCTGATGAGAAAGAAGGAGATCTTCCCATCAGTACCTTGAAACTATTGACTTCCAAAGAGTCAAAAACTGAAAACACCCAATATTATATCCTAAGCTGGCCGGAAAAGAAAGCATGTCAAATTACAAATTTCCCTCATCCGTATCCACAACTGGCATCATTGCAGAAAGAGATGGTCAGGTACCAGAGAAAGGATGGGGTTCAACTAACTGCAACACTATATCTTCCACCAGGCTACGATCCATCCAGAGATGGCCCTCTTCCATGTTTGGTTTGGTCTTATCCTGGAGAATTCAAAAGCAAAGATGCTGCCGGACAAGTTCGTGGTTCTCCTAATGAATTTGCTGGGATAGGTCCAACATCAGCTCTGCTCTGGATGGCTCGAAGGTTTGCTATTTTATCTGGACCAACTATTCCTATTATTGGTGAGGGTGATGATGAGGCAAATGATAGGTATGTGGAGCAATTGGTAGCAAGTGCAGAGGCTGCTGTGGAGGAAGTCATTCGACGTGGAGTGGCTCATCCAAAGAAAATTGCTGTTGGGGGACATTCATATGGGGCATTCATGACTGCAAACCTACTGGCACATGCTCCTCATCTTTTTGCTTGTGGAATTGCTCGTTCGGGTGCTTACAACAGAACACTTACTCCTTTCGGTTTTCAGAATGAGGAGAGAACTCTTTGGGAGGCTACTAGTACTTATGTGGAGATGAGTCCTTTCATGTCAGCTAATAAAATTAAGAAGCCAATCTTGCTTATCCATGGAGAGGAAGACAATAATCCAGGGACTTTAACCATGCAGTCTGATCGTTTCTTTAATGCTCTGAAAGGTCATGGTGCTCTTTGCCGCCTTGTGATTCTTCCCTTTGAGAGCCATGGTTATGCTGCAAGAGAGAGTATCATGCATGTGCTATGGGAAACTGACAGGTGGTTGCAAAAATATTGTGTGTCAGACACTTCTGATGTAAATGTTAATCAAGATGCATGTAAAGACAATATAGGCACAGGATCTACAAACTCTGAAAGCAAACCAGTGGCTACTGGCGGGGGCAGTGGCTCAGAAGTGTCAAATTCTGAGCATGAAGGAGTTGACTCTTTGCCGAGGTCATTGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

964

Amino Acids

107.27

Weight (kDa)

5.9

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 754 - 907 2.2e-22 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 508
Acc65I GGTACC 1 cut(s) 1959
AccB1I GGYRCC 2 cut(s) 853, 1959
AccBSI CCGCTC 1 cut(s) 1253
AccI GTMKAC 1 cut(s) 182
AccII CGCG 2 cut(s) 118, 136
AclWI GGATC 7 cut(s) 526, 539, 1364, 1504, 1550, 2012, 2794
AcoI YGGCCR 2 cut(s) 131, 1877
AcsI RAATTY 5 cut(s) 870, 1906, 2069, 2117, 2848
AcuI CTGAAG 2 cut(s) 431, 947
AcvI CACGTG 1 cut(s) 1325
AfaI GTAC 7 cut(s) 556, 579, 1107, 1457, 1813, 1961, 2476
AfiI CCNNNNNNNGG 6 cut(s) 1361, 1575, 1674, 1969, 2201, 2545
AhlI ACTAGT 2 cut(s) 674, 2471
AjiI CACGTC 1 cut(s) 2288
AjnI CCWGG 8 cut(s) 722, 915, 1478, 1492, 1558, 2008, 2062, 2560
AleI CACNNNNGTG 1 cut(s) 1598
AloI GAACNNNNNNTCC 2 cut(s) 2410, 2442
Alw21I GWGCWC 1 cut(s) 2620
Alw26I GTCTC 2 cut(s) 265, 1277
AlwI GGATC 7 cut(s) 526, 539, 1364, 1504, 1550, 2012, 2794
AlwNI CAGNNNCTG 3 cut(s) 1025, 1559, 2264
AoxI GGCC 7 cut(s) 131, 369, 642, 725, 863, 1877, 2032
ApeKI GCWGC 4 cut(s) 1573, 2087, 2264, 2663
ApoI RAATTY 5 cut(s) 870, 1906, 2069, 2117, 2848
Asp700I GAANNNNTTC 1 cut(s) 2280
Asp718I GGTACC 1 cut(s) 1959
AspLEI GCGC 2 cut(s) 120, 1236
AsuHPI GGTGA 5 cut(s) 796, 1015, 1376, 2216, 2222
AvaII GGWCC 8 cut(s) 153, 621, 909, 1016, 1042, 1419, 2132, 2183
AxyI CCTNAGG 1 cut(s) 591
BaeI ACNNNNGTAYC 2 cut(s) 569, 602
BamHI GGATCC 1 cut(s) 531
BanI GGYRCC 2 cut(s) 853, 1959
BarI GAAGNNNNNNTAC 2 cut(s) 561, 593
BbrPI CACGTG 1 cut(s) 1325
BbsI GAAGAC 2 cut(s) 314, 2556
Bbv12I GWGCWC 1 cut(s) 2620
BbvI GCAGC 4 cut(s) 1560, 2074, 2251, 2650
BceAI ACGGC 5 cut(s) 131, 158, 212, 259, 415
BcgI CGANNNNNNTGC 2 cut(s) 2567, 2601
BciT130I CCWGG 8 cut(s) 724, 917, 1480, 1494, 1560, 2010, 2064, 2562
BciVI GTATCC 1 cut(s) 1932
BcoDI GTCTC 2 cut(s) 265, 1277
BcuI ACTAGT 2 cut(s) 674, 2471
BfaI CTAG 8 cut(s) 186, 192, 198, 675, 1358, 1433, 1439, 2472
BfuI GTATCC 1 cut(s) 1932
BglI GCCNNNNNGGC 1 cut(s) 862
BglII AGATCT 1 cut(s) 1798
BlpI GCTNAGC 1 cut(s) 203
BmcAI AGTACT 1 cut(s) 2476
Bme1390I CCNGG 8 cut(s) 724, 917, 1480, 1494, 1560, 2010, 2064, 2562
Bme18I GGWCC 8 cut(s) 153, 621, 909, 1016, 1042, 1419, 2132, 2183
BmgBI CACGTC 1 cut(s) 2288
BmrFI CCNGG 8 cut(s) 724, 917, 1480, 1494, 1560, 2010, 2064, 2562
BmrI ACTGGG 3 cut(s) 531, 723, 1285
BmsI GCATC 8 cut(s) 3, 343, 1221, 1291, 1527, 1943, 2074, 2749
BmuI ACTGGG 3 cut(s) 531, 723, 1285
BpiI GAAGAC 2 cut(s) 314, 2556
BpmI CTGGAG 2 cut(s) 1267, 2085
Bpu10I CCTNAGC 1 cut(s) 1871
Bpu1102I GCTNAGC 1 cut(s) 203
BsaAI YACGTR 1 cut(s) 1325
BsaI GGTCTC 1 cut(s) 1277
BsaJI CCNNGG 9 cut(s) 228, 237, 826, 1191, 1479, 2539, 2561, 2653, 2879
BsaXI ACNNNNNCTCC 6 cut(s) 57, 87, 1380, 1410, 2410, 2440
Bsc4I CCNNNNNNNGG 6 cut(s) 1361, 1575, 1674, 1969, 2201, 2545
Bse1I ACTGG 8 cut(s) 526, 718, 1250, 1280, 1935, 2364, 2810, 2824
Bse21I CCTNAGG 1 cut(s) 591
Bse3DI GCAATG 2 cut(s) 1203, 1937
BseBI CCWGG 8 cut(s) 724, 917, 1480, 1494, 1560, 2010, 2064, 2562
BseDI CCNNGG 9 cut(s) 228, 237, 826, 1191, 1479, 2539, 2561, 2653, 2879
BseLI CCNNNNNNNGG 6 cut(s) 1361, 1575, 1674, 1969, 2201, 2545
BseMI GCAATG 2 cut(s) 1203, 1937
BseMII CTCAG 4 cut(s) 217, 582, 2844, 2850
BseNI ACTGG 8 cut(s) 526, 718, 1250, 1280, 1935, 2364, 2810, 2824
BseRI GAGGAG 3 cut(s) 470, 2361, 2462
BseX3I CGGCCG 1 cut(s) 131
BseXI GCAGC 4 cut(s) 1560, 2074, 2251, 2650
BseYI CCCAGC 1 cut(s) 2123
BsgI GTGCAG 1 cut(s) 2277
Bsh1236I CGCG 2 cut(s) 118, 136
Bsh1285I CGRYCG 1 cut(s) 134
BshFI GGCC 7 cut(s) 133, 371, 644, 727, 865, 1879, 2034
BshNI GGYRCC 2 cut(s) 853, 1959
BsiEI CGRYCG 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 2620
BsiSI CCGG 4 cut(s) 368, 548, 1880, 2091
BslFI GGGAC 6 cut(s) 200, 607, 808, 1497, 2337, 2578
BslI CCNNNNNNNGG 6 cut(s) 1361, 1575, 1674, 1969, 2201, 2545
BsmAI GTCTC 2 cut(s) 265, 1277
BsmBI CGTCTC 1 cut(s) 265
BsmFI GGGAC 6 cut(s) 200, 607, 808, 1497, 2337, 2578
BsmI GAATGC 3 cut(s) 763, 1208, 2339
BsnI GGCC 7 cut(s) 133, 371, 644, 727, 865, 1879, 2034
Bso31I GGTCTC 1 cut(s) 1277
Bsp1286I GDGCHC 1 cut(s) 2620
Bsp1720I GCTNAGC 1 cut(s) 203
Bsp19I CCATGG 2 cut(s) 2539, 2653
BspANI GGCC 7 cut(s) 133, 371, 644, 727, 865, 1879, 2034
BspCNI CTCAG 4 cut(s) 216, 583, 2845, 2849
BspFNI CGCG 2 cut(s) 118, 136
BspHI TCATGA 1 cut(s) 2343
BspPI GGATC 7 cut(s) 526, 539, 1364, 1504, 1550, 2012, 2794
BspT107I GGYRCC 2 cut(s) 853, 1959
BspTNI GGTCTC 1 cut(s) 1277
BsrBI CCGCTC 1 cut(s) 1253
BsrDI GCAATG 2 cut(s) 1203, 1937
BsrI ACTGG 8 cut(s) 526, 718, 1250, 1280, 1935, 2364, 2810, 2824
BssECI CCNNGG 9 cut(s) 228, 237, 826, 1191, 1479, 2539, 2561, 2653, 2879
BssT1I CCWWGG 2 cut(s) 2539, 2653
Bst2UI CCWGG 8 cut(s) 724, 917, 1480, 1494, 1560, 2010, 2064, 2562
Bst4CI ACNGT 3 cut(s) 254, 405, 752
Bst6I CTCTTC 6 cut(s) 54, 268, 799, 1219, 1730, 2043
BstAPI GCANNNNNTGC 1 cut(s) 2264
BstBAI YACGTR 1 cut(s) 1325
BstC8I GCNNGC 5 cut(s) 465, 1347, 1591, 1605, 1877
BstDEI CTNAG 6 cut(s) 203, 591, 600, 1871, 2836, 2853
BstDSI CCRYGG 4 cut(s) 228, 826, 2539, 2653
BstFNI CGCG 2 cut(s) 118, 136
BstHHI GCGC 2 cut(s) 120, 1236
BstMAI GTCTC 2 cut(s) 265, 1277
BstMCI CGRYCG 1 cut(s) 134
BstMWI GCNNNNNNNGC 4 cut(s) 233, 862, 2264, 2530
BstNI CCWGG 8 cut(s) 724, 917, 1480, 1494, 1560, 2010, 2064, 2562
BstNSI RCATGY 4 cut(s) 1896, 2369, 2687, 2766
BstSCI CCNGG 8 cut(s) 722, 915, 1478, 1492, 1558, 2008, 2062, 2560
BstUI CGCG 2 cut(s) 118, 136
BstV1I GCAGC 4 cut(s) 1560, 2074, 2251, 2650
BstV2I GAAGAC 2 cut(s) 314, 2556
BstX2I RGATCY 5 cut(s) 531, 1369, 1555, 1798, 2786
BstYI RGATCY 5 cut(s) 531, 1369, 1555, 1798, 2786
BstZI CGGCCG 1 cut(s) 131
Bsu36I CCTNAGG 1 cut(s) 591
BsuI GTATCC 1 cut(s) 1932
BsuRI GGCC 7 cut(s) 133, 371, 644, 727, 865, 1879, 2034
BtgI CCRYGG 4 cut(s) 228, 826, 2539, 2653
BtgZI GCGATG 1 cut(s) 23
BtrI CACGTC 1 cut(s) 2288
BtsI GCAGTG 2 cut(s) 270, 2836
BtsIMutI CAGTG 3 cut(s) 270, 2817, 2836
Cac8I GCNNGC 5 cut(s) 465, 1347, 1591, 1605, 1877
CaiI CAGNNNCTG 3 cut(s) 1025, 1559, 2264
CciI TCATGA 1 cut(s) 2343
CciNI GCGGCCGC 1 cut(s) 131
CfoI GCGC 2 cut(s) 120, 1236
Csp6I GTAC 7 cut(s) 555, 578, 1106, 1456, 1812, 1960, 2475
CviQI GTAC 7 cut(s) 555, 578, 1106, 1456, 1812, 1960, 2475
DdeI CTNAG 6 cut(s) 203, 591, 600, 1871, 2836, 2853
DrdI GACNNNNNNGTC 1 cut(s) 508
DseDI GACNNNNNNGTC 1 cut(s) 508
EaeI YGGCCR 2 cut(s) 131, 1877
EagI CGGCCG 1 cut(s) 131
Eam1104I CTCTTC 6 cut(s) 54, 268, 799, 1219, 1730, 2043
EarI CTCTTC 6 cut(s) 54, 268, 799, 1219, 1730, 2043
EciI GGCGGA 1 cut(s) 348
EclXI CGGCCG 1 cut(s) 131
Eco130I CCWWGG 2 cut(s) 2539, 2653
Eco147I AGGCCT 1 cut(s) 727
Eco31I GGTCTC 1 cut(s) 1277
Eco32I GATATC 1 cut(s) 317
Eco47I GGWCC 8 cut(s) 153, 621, 909, 1016, 1042, 1419, 2132, 2183
Eco52I CGGCCG 1 cut(s) 131
Eco57I CTGAAG 2 cut(s) 431, 947
Eco72I CACGTG 1 cut(s) 1325
Eco81I CCTNAGG 1 cut(s) 591
EcoO109I RGGNCCY 1 cut(s) 909
EcoRI GAATTC 1 cut(s) 2069
EcoRII CCWGG 8 cut(s) 722, 915, 1478, 1492, 1558, 2008, 2062, 2560
EcoRV GATATC 1 cut(s) 317
EcoT14I CCWWGG 2 cut(s) 2539, 2653
EcoT22I ATGCAT 2 cut(s) 2685, 2764
ErhI CCWWGG 2 cut(s) 2539, 2653
Esp3I CGTCTC 1 cut(s) 265
FalI AAGNNNNNCTT 4 cut(s) 1785, 1817, 2513, 2545
FaqI GGGAC 6 cut(s) 200, 607, 808, 1497, 2337, 2578
FauI CCCGC 2 cut(s) 1523, 2816
FauNDI CATATG 2 cut(s) 1597, 2332
FblI GTMKAC 1 cut(s) 182
FspBI CTAG 8 cut(s) 186, 192, 198, 675, 1358, 1433, 1439, 2472
GlaI GCGC 2 cut(s) 119, 1235
GsaI CCCAGC 1 cut(s) 2127
GsuI CTGGAG 2 cut(s) 1267, 2085
HaeIII GGCC 7 cut(s) 133, 371, 644, 727, 865, 1879, 2034
HapII CCGG 4 cut(s) 368, 548, 1880, 2091
HhaI GCGC 2 cut(s) 120, 1236
Hin6I GCGC 2 cut(s) 118, 1234
HinP1I GCGC 2 cut(s) 118, 1234
HincII GTYRAC 2 cut(s) 435, 2869
HindII GTYRAC 2 cut(s) 435, 2869
HindIII AAGCTT 2 cut(s) 461, 683
HpaII CCGG 4 cut(s) 368, 548, 1880, 2091
HphI GGTGA 5 cut(s) 796, 1015, 1376, 2216, 2222
Hpy166II GTNNAC 6 cut(s) 183, 435, 787, 1143, 1444, 2869
Hpy188III TCNNGA 6 cut(s) 907, 2025, 2152, 2181, 2344, 2756
Hpy8I GTNNAC 6 cut(s) 183, 435, 787, 1143, 1444, 2869
Hpy99I CGWCG 1 cut(s) 2289
HpyCH4III ACNGT 3 cut(s) 254, 405, 752
HpyCH4IV ACGT 3 cut(s) 308, 1324, 2287
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 862, 2264, 2530
HpyF3I CTNAG 6 cut(s) 203, 591, 600, 1871, 2836, 2853
HpySE526I ACGT 3 cut(s) 308, 1324, 2287
HspAI GCGC 2 cut(s) 118, 1234
KpnI GGTACC 1 cut(s) 1963
LmnI GCTCC 5 cut(s) 59, 361, 1568, 2239, 2374
Lsp1109I GCAGC 4 cut(s) 1560, 2074, 2251, 2650
LweI GCATC 8 cut(s) 3, 343, 1221, 1291, 1527, 1943, 2074, 2749
MaeI CTAG 8 cut(s) 186, 192, 198, 675, 1358, 1433, 1439, 2472
MaeII ACGT 3 cut(s) 308, 1324, 2287
MaeIII GTNAC 3 cut(s) 613, 738, 1739
MbiI CCGCTC 1 cut(s) 1253
MfeI CAATTG 2 cut(s) 539, 2243
MflI RGATCY 5 cut(s) 531, 1369, 1555, 1798, 2786
MhlI GDGCHC 1 cut(s) 2620
MlyI GAGTC 7 cut(s) 496, 746, 785, 1457, 1847, 2500, 2864
MmeI TCCRAC 2 cut(s) 1397, 2159
Mph1103I ATGCAT 2 cut(s) 2685, 2764
MroXI GAANNNNTTC 1 cut(s) 2280
MseI TTAA 8 cut(s) 339, 687, 1070, 1775, 2517, 2570, 2595, 2751
MslI CAYNNNNRTG 5 cut(s) 1598, 2331, 2658, 2684, 2890
MspA1I CMGCKG 2 cut(s) 1022, 1148
MspI CCGG 4 cut(s) 368, 548, 1880, 2091
MspR9I CCNGG 8 cut(s) 724, 917, 1480, 1494, 1560, 2010, 2064, 2562
MunI CAATTG 2 cut(s) 539, 2243
Mva1269I GAATGC 3 cut(s) 763, 1208, 2339
MvaI CCWGG 8 cut(s) 724, 917, 1480, 1494, 1560, 2010, 2064, 2562
MvnI CGCG 2 cut(s) 118, 136
MwoI GCNNNNNNNGC 4 cut(s) 233, 862, 2264, 2530
NcoI CCATGG 2 cut(s) 2539, 2653
NdeI CATATG 2 cut(s) 1597, 2332
NmeAIII GCCGAG 2 cut(s) 262, 1216
NmuCI GTSAC 2 cut(s) 738, 1739
NotI GCGGCCGC 1 cut(s) 131
NsiI ATGCAT 2 cut(s) 2685, 2764
NspI RCATGY 4 cut(s) 1896, 2369, 2687, 2766
OliI CACNNNNGTG 1 cut(s) 1598
PagI TCATGA 1 cut(s) 2343
PceI AGGCCT 1 cut(s) 727
PctI GAATGC 3 cut(s) 763, 1208, 2339
PdmI GAANNNNTTC 1 cut(s) 2280
PfeI GAWTC 3 cut(s) 302, 1427, 2635
PfoI TCCNGGA 3 cut(s) 915, 1492, 2062
PleI GAGTC 7 cut(s) 496, 745, 785, 1456, 1846, 2499, 2864
PmaCI CACGTG 1 cut(s) 1325
PmlI CACGTG 1 cut(s) 1325
PpsI GAGTC 7 cut(s) 496, 745, 785, 1456, 1846, 2499, 2864
Ppu21I YACGTR 1 cut(s) 1325
PpuMI RGGWCCY 1 cut(s) 909
Psp5II RGGWCCY 1 cut(s) 909
Psp6I CCWGG 8 cut(s) 722, 915, 1478, 1492, 1558, 2008, 2062, 2560
PspCI CACGTG 1 cut(s) 1325
PspFI CCCAGC 1 cut(s) 2123
PspGI CCWGG 8 cut(s) 722, 915, 1478, 1492, 1558, 2008, 2062, 2560
PspPPI RGGWCCY 1 cut(s) 909
PsrI GAACNNNNNNTAC 2 cut(s) 547, 579
PstNI CAGNNNCTG 3 cut(s) 1025, 1559, 2264
PsuI RGATCY 5 cut(s) 531, 1369, 1555, 1798, 2786
PvuII CAGCTG 1 cut(s) 1148
RsaI GTAC 7 cut(s) 556, 579, 1107, 1457, 1813, 1961, 2476
RsaNI GTAC 7 cut(s) 555, 578, 1106, 1456, 1812, 1960, 2475
RseI CAYNNNNRTG 5 cut(s) 1598, 2331, 2658, 2684, 2890
SaqAI TTAA 8 cut(s) 339, 687, 1070, 1775, 2517, 2570, 2595, 2751
ScaI AGTACT 1 cut(s) 2476
SchI GAGTC 7 cut(s) 496, 746, 785, 1457, 1847, 2500, 2864
ScrFI CCNGG 8 cut(s) 724, 917, 1480, 1494, 1560, 2010, 2064, 2562
SduI GDGCHC 1 cut(s) 2620
SfaNI GCATC 8 cut(s) 3, 343, 1221, 1291, 1527, 1943, 2074, 2749
SinI GGWCC 8 cut(s) 153, 621, 909, 1016, 1042, 1419, 2132, 2183
SmiMI CAYNNNNRTG 5 cut(s) 1598, 2331, 2658, 2684, 2890
SpeI ACTAGT 2 cut(s) 674, 2471
SseBI AGGCCT 1 cut(s) 727
SspI AATATT 2 cut(s) 1862, 2720
SspMI CTAG 8 cut(s) 186, 192, 198, 675, 1358, 1433, 1439, 2472
StuI AGGCCT 1 cut(s) 727
StyD4I CCNGG 8 cut(s) 722, 915, 1478, 1492, 1558, 2008, 2062, 2560
StyI CCWWGG 2 cut(s) 2539, 2653
TaaI ACNGT 3 cut(s) 254, 405, 752
TaiI ACGT 3 cut(s) 311, 1327, 2290
TaqI TCGA 4 cut(s) 655, 1692, 2161, 2284
TatI WGTACW 1 cut(s) 2474
TfiI GAWTC 3 cut(s) 302, 1427, 2635
Tru1I TTAA 8 cut(s) 339, 687, 1070, 1775, 2517, 2570, 2595, 2751
Tru9I TTAA 8 cut(s) 339, 687, 1070, 1775, 2517, 2570, 2595, 2751
TscAI CASTG 3 cut(s) 277, 2817, 2836
TseFI GTSAC 2 cut(s) 738, 1739
TseI GCWGC 4 cut(s) 1573, 2087, 2264, 2663
Tsp45I GTSAC 2 cut(s) 738, 1739
TspGWI ACGGA 6 cut(s) 95, 113, 247, 1388, 1480, 1908
TspRI CASTG 3 cut(s) 277, 2817, 2836
VpaK11BI GGWCC 8 cut(s) 153, 621, 909, 1016, 1042, 1419, 2132, 2183
XapI RAATTY 5 cut(s) 870, 1906, 2069, 2117, 2848
XceI RCATGY 4 cut(s) 1896, 2369, 2687, 2766
XcmI CCANNNNNNNNNTGG 3 cut(s) 710, 1970, 2049
XmiI GTMKAC 1 cut(s) 182
XmnI GAANNNNTTC 1 cut(s) 2280
XspI CTAG 8 cut(s) 186, 192, 198, 675, 1358, 1433, 1439, 2472
ZrmI AGTACT 1 cut(s) 2476
Zsp2I ATGCAT 2 cut(s) 2685, 2764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.