MD03G1294700.v1.1

Glutamyl endopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002809
Physical Location & Seq
Reverse (-)
37073565 .. 37078576
5012 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1294700.v1.1.491

Sequence Viewer

Length: 2919 bp
ATGAATGTCGATAGGATGTTCCTTCACAAAGTCTATCATCGCCTCTCCCTCCTCTCCCTCACTCCCCCTCACTCACTCCCTTTCAAGCCTCTCCACTTCACATCGGTCCTTTCGCCCGCCCACCTCAGAACAAGGCGCTCCCCATACGACGCCGTAAGTTCCATCAGAACATCCGCCGCCGCCATGGCCACCTCTCGGCTCCGCAACCTCGTCCCCGTCAACGCCGTCGCTTCCGAAGATTCCAACAGTGGCGGAGCCACCAACGGCTCTGTCTCTTCCACTGCAGCTGCCACTTCACTTGACGATGAAGAGGATGCGACTTTGGGAGTTAGATACCGTCTTCCTCCGCAAGAAATCAAAGATATTGTGGATGCTCCGCCACTTCCCGCCTTGTCATTCTCACCGCATAGGGATAAGATACTGTTTCTCAAGCGGAGAGCGTTGCCGCCATTGGCAGAACTTGCAAGACCCGAGGAAAAGCTTGCCGGTGTTCGTATTGATGGAAAATGCAATACAAGGACGAGGATGTCGTTTTACACGGGTATTGGGATCCATCAATTATTGCCTGATGGCACCCTTGGTCCGGAGATAGAGGTGCGTGGATTCCCTGATGGTGCCAAGATCAATTTTGTTACTTGGTCCCCGGATGGCCGCCATTTAGCCTTCACCATCCGGTTTGATGAGGGGGAAAGTACTACTAGTAAGCTTAAAGTGTGGGTGGCCCAAGTTGAAACAGGGATTGCTAAACCTTTGTTTAAGTCGGAAGAAATATTTTTGAATGCAGTTTTTGATAATTTTGTTTGGGTGAATGATTCTTCTTTGTTAGTTTGCACCATTCCTCAGTCTCGTGGTGACCCGCCAAAGAAACCTTTGGTTCCTTTTGGGCCTAAAATTCAATCTAATGAGCAGAAAAGTATTATCCAAGTTAGGACCTTTCAGGATTTGCTGAAGGATGAGTATGATGAAGATTTGTTTGACTACTACGCCACGACACAACTTGTTTTGGCCTCCTTGGATGGGGCAGTTAAGGAAATTGGTTCTCCAGCTGTATATACATCAATGGACCCCTCTCCCGATCAGAAATACCTCTTAATTAGTTCAATTCACAGACCATACTCTTTCATTGTACCATGTGGAAGATTTCCCAAGAAGGTGGATGTGTGGACAGCTGATGGGAAGTTTGTTAGAGAAATTTGTGATCTGCCCCTTGCTGAGGACATCCCCATTGCATTCAGTAGTGTGCGAAAGGGGAAGCGGTCTATCAACTGGAGAGCAGATAAACCATCAACACTCTATTGGGTTGAGACCCAAGATGAAGGAAATGCAAAAGTGGATGTTTCTCCACGTGATATAGTTTATACACAGTCCGCTGAGCCACTTGAAGGTGAAGGGCCAACTGTCCTACACAAACTTGATCTTCGCTATGGAGGGATTTCTTGGTCAGATGATTCACTGGCTCTAGTTTACGAGTCTTGGTACAAAACACGGAGAACGAGAACCTGGGTAATTTCTCCTGGATCAAATGATGTCAGTCCACGCATCCTGTTTGATAGATCGTCAGAAGATGTGTATTCTGATCCTGGCTCTCCTATGCTGCGGAGAACTCCTGCTGGGACATATGTGTTAGCAAAAGTAAAGAAAGAAAATGAGGAAGGCACTTATTTACTGCTGAATGGAAATGGTGCTACACCAGAGGGAGATATCCCCTTCCTCGACTTGTTTGACATAAATACAGGAAACAAAGAAAGAATATGGAAGAGTGACAAAGAAAAGTATTACGAGACTGTTGTTGCTTTAATGTCTGATGAGGAAGAAGGGGATCTGCTCGTTGATCACCTGAAAGTATTGACTTCCAAAGAGTCAAAAACTGAAAACACCCAGTATTCTATCCTAAGCTGGCCAGAAAAGAAAGCAGTCCAAATTACAAATTTTCCTCATCCATATCCACAGCTGGCATCATTGCAGAAAGAGCTGGTCAAGTACCAGAGGAAGGATGGGGTTCAACTAACTGCAACGCTATACCTTCCACCAGGTTATGATCCAGCCAGAGATGGCCCTCTTCCATGTCTGTTCTGGTCTTACCCTGGAGAATTCAAGAGCAAAGATGCTGCTGGACAAGTTCGTGGTTCTCCTAATGAATTTGCTGGCATAGGTCCCACATCAGCCCTCCTCTGGCTGGCTCGAAGGTTTGCTATTCTGTCTGGACCAACAATTCCTATTATTGGTGAGGGTGATAAAGAAGCAAATGATAGCTATGTGGAGCAATTGGTTGCAAGTGCAGAGGCTGCTGTGGAGGAAGTTGTTCGACGCGGGGTGGCTCATCCAGACAAAATTGCTGTTGGGGGACATTCATATGGGGCATTCATGACTGCAAACCTCTTGGCACATGCCCCTCATCTTTTTTCTTGTGGAATTGCTCGTTCTGGTGCTTACAACAGAACACTTACTCCTTTTGGTTTTCAGAACGAGGATAGAACTCTTTGGGAGGCTACCAGTACTTATGTAGAGATGAGTCCCTTCATGTCCGCTAATAAAATTAAGAAGCCAATCTTGCTTATCCATGGAGAAGAAGACAATAATTCTGGAACTTTGACAATGCAGTCAGATCGCTTCTTTAATGCTCTGAAAGGTCATGGTGCTCTGTCCCGCCTCGTGATTCTTCCATTTGAGAGCCATGGATATGCAGCAAGAGAGAGCATCATGCACGTGCTATGGGAAAGTGATAGATGGCTTCAGAAATATTGCGTGTCAAACACTTCCAATGTAAATGCAGATTCCGATGCAAGTAAAGAGAACTTAAGCCCAGGATCTACAGTTTCTGAAAGCAAATCGGTGGCTGCTAGTGGAGGCGGTAGTCCAGAGGTGTCAAATTCTGAGCATGAAGGATTTGACTCATTGCCAAGGTCATCGTTATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

973

Amino Acids

107.6

Weight (kDa)

5.81

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 761 - 914 4.7e-22 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 526, 2599
AccB1I GGYRCC 2 cut(s) 572, 614
AccII CGCG 1 cut(s) 2310
AccIII TCCGGA 1 cut(s) 583
AclWI GGATC 7 cut(s) 544, 557, 1525, 1571, 1827, 2033, 2815
AcoI YGGCCR 3 cut(s) 186, 649, 1898
AcsI RAATTY 6 cut(s) 891, 1191, 1927, 2090, 2138, 2869
AcuI CTGAAG 2 cut(s) 968, 2717
AcvI CACGTG 2 cut(s) 1346, 2707
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 5 cut(s) 694, 1128, 1478, 1982, 2497
AfiI CCNNNNNNNGG 9 cut(s) 195, 583, 1017, 1213, 1382, 1990, 2172, 2176, 2222
AflII CTTAAG 1 cut(s) 2797
AgsI TTSAA 8 cut(s) 85, 731, 778, 896, 1101, 1382, 2003, 2095
AhlI ACTAGT 1 cut(s) 698
AjnI CCWGG 6 cut(s) 1499, 1513, 1579, 2029, 2083, 2803
AloI GAACNNNNNNTCC 2 cut(s) 2431, 2463
AluBI AGCT 9 cut(s) 287, 481, 706, 1046, 1169, 1896, 1951, 1972, 2253
AluI AGCT 9 cut(s) 287, 481, 706, 1046, 1169, 1896, 1951, 1972, 2253
Alw21I GWGCWC 1 cut(s) 2641
Alw26I GTCTC 4 cut(s) 277, 849, 1298, 1775
AlwI GGATC 7 cut(s) 544, 557, 1525, 1571, 1827, 2033, 2815
AlwNI CAGNNNCTG 2 cut(s) 2285, 2819
Ama87I CYCGRG 1 cut(s) 470
Aor13HI TCCGGA 1 cut(s) 583
AoxI GGCC 8 cut(s) 186, 649, 720, 884, 1005, 1391, 1898, 2053
ApeKI GCWGC 7 cut(s) 284, 287, 1594, 2108, 2285, 2684, 2837
ApoI RAATTY 6 cut(s) 891, 1191, 1927, 2090, 2138, 2869
Asp700I GAANNNNTTC 1 cut(s) 2301
AspLEI GCGC 1 cut(s) 138
AsuC2I CCSGG 1 cut(s) 644
AsuHPI GGTGA 8 cut(s) 393, 658, 817, 863, 1397, 1826, 2237, 2243
AvaI CYCGRG 1 cut(s) 470
AvaII GGWCC 7 cut(s) 106, 581, 639, 930, 1063, 2153, 2204
BalI TGGCCA 2 cut(s) 188, 1900
BamHI GGATCC 1 cut(s) 549
BanI GGYRCC 2 cut(s) 572, 614
BauI CACGAG 2 cut(s) 846, 2651
BbrPI CACGTG 2 cut(s) 1346, 2707
BbsI GAAGAC 2 cut(s) 332, 2577
Bbv12I GWGCWC 1 cut(s) 2641
BbvCI CCTCAGC 1 cut(s) 1212
BbvI GCAGC 7 cut(s) 274, 296, 1581, 2095, 2272, 2696, 2824
BceAI ACGGC 3 cut(s) 137, 209, 280
BcgI CGANNNNNNTGC 2 cut(s) 2588, 2622
BciT130I CCWGG 6 cut(s) 1501, 1515, 1581, 2031, 2085, 2805
BclI TGATCA 1 cut(s) 1831
BcnI CCSGG 1 cut(s) 644
BcoDI GTCTC 4 cut(s) 277, 849, 1298, 1775
BcuI ACTAGT 1 cut(s) 698
BfaI CTAG 3 cut(s) 699, 1460, 2841
BfmI CTRYAG 2 cut(s) 282, 2811
BfoI RGCGCY 1 cut(s) 139
BfrI CTTAAG 1 cut(s) 2797
BglI GCCNNNNNGGC 1 cut(s) 185
BlpI GCTNAGC 1 cut(s) 1371
BmcAI AGTACT 2 cut(s) 694, 2497
Bme1390I CCNGG 7 cut(s) 644, 1501, 1515, 1581, 2031, 2085, 2805
Bme18I GGWCC 7 cut(s) 106, 581, 639, 930, 1063, 2153, 2204
BmeT110I CYCGRG 1 cut(s) 470
BmiI GGNNCC 9 cut(s) 200, 256, 551, 574, 616, 641, 876, 1065, 2155
BmrFI CCNGG 7 cut(s) 644, 1501, 1515, 1581, 2031, 2085, 2805
BmrI ACTGGG 1 cut(s) 1873
BmsI GCATC 7 cut(s) 304, 361, 1548, 1964, 2095, 2706, 2770
BmuI ACTGGG 1 cut(s) 1873
BpiI GAAGAC 2 cut(s) 332, 2577
BpmI CTGGAG 3 cut(s) 1026, 1288, 2106
Bpu10I CCTNAGC 2 cut(s) 1212, 1892
Bpu1102I GCTNAGC 1 cut(s) 1371
BpuEI CTTGAG 1 cut(s) 413
BpuMI CCSGG 1 cut(s) 644
BsaAI YACGTR 2 cut(s) 1346, 2707
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 1298
BsaWI WCCGGW 2 cut(s) 583, 672
BsaXI ACNNNNNCTCC 2 cut(s) 2431, 2461
Bsc4I CCNNNNNNNGG 9 cut(s) 195, 583, 1017, 1213, 1382, 1990, 2172, 2176, 2222
Bse118I RCCGGY 1 cut(s) 485
Bse1I ACTGG 4 cut(s) 1271, 1458, 1879, 2493
Bse3DI GCAATG 3 cut(s) 1224, 1958, 2894
BseAI TCCGGA 1 cut(s) 583
BseBI CCWGG 6 cut(s) 1501, 1515, 1581, 2031, 2085, 2805
BseLI CCNNNNNNNGG 9 cut(s) 195, 583, 1017, 1213, 1382, 1990, 2172, 2176, 2222
BseMI GCAATG 3 cut(s) 1224, 1958, 2894
BseMII CTCAG 5 cut(s) 139, 854, 1203, 1362, 2865
BseNI ACTGG 4 cut(s) 1271, 1458, 1879, 2493
BseRI GAGGAG 2 cut(s) 41, 2159
BseXI GCAGC 7 cut(s) 274, 296, 1581, 2095, 2272, 2696, 2824
BseYI CCCAGC 1 cut(s) 1610
BsgI GTGCAG 1 cut(s) 2298
Bsh1236I CGCG 1 cut(s) 2310
BshFI GGCC 8 cut(s) 188, 651, 722, 886, 1007, 1393, 1900, 2055
BshNI GGYRCC 2 cut(s) 572, 614
BsiHKAI GWGCWC 1 cut(s) 2641
BsiHKCI CYCGRG 1 cut(s) 470
BsiSI CCGG 4 cut(s) 486, 584, 644, 673
BslFI GGGAC 7 cut(s) 197, 625, 1627, 2139, 2358, 2499, 2629
BslI CCNNNNNNNGG 9 cut(s) 195, 583, 1017, 1213, 1382, 1990, 2172, 2176, 2222
BsmAI GTCTC 4 cut(s) 277, 849, 1298, 1775
BsmFI GGGAC 7 cut(s) 197, 625, 1627, 2139, 2358, 2499, 2629
BsmI GAATGC 3 cut(s) 784, 1229, 2360
BsnI GGCC 8 cut(s) 188, 651, 722, 886, 1007, 1393, 1900, 2055
Bso31I GGTCTC 1 cut(s) 1298
BsoBI CYCGRG 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 2641
Bsp13I TCCGGA 1 cut(s) 583
Bsp1720I GCTNAGC 1 cut(s) 1371
Bsp19I CCATGG 3 cut(s) 183, 2560, 2674
BspANI GGCC 8 cut(s) 188, 651, 722, 886, 1007, 1393, 1900, 2055
BspCNI CTCAG 5 cut(s) 138, 853, 1204, 1363, 2866
BspEI TCCGGA 1 cut(s) 583
BspFNI CGCG 1 cut(s) 2310
BspHI TCATGA 1 cut(s) 2364
BspLI GGNNCC 9 cut(s) 200, 256, 551, 574, 616, 641, 876, 1065, 2155
BspMAI CTGCAG 1 cut(s) 286
BspPI GGATC 7 cut(s) 544, 557, 1525, 1571, 1827, 2033, 2815
BspT107I GGYRCC 2 cut(s) 572, 614
BspTI CTTAAG 1 cut(s) 2797
BspTNI GGTCTC 1 cut(s) 1298
BsrDI GCAATG 3 cut(s) 1224, 1958, 2894
BsrFI RCCGGY 1 cut(s) 485
BsrI ACTGG 4 cut(s) 1271, 1458, 1879, 2493
BssAI RCCGGY 1 cut(s) 485
BssNI GRCGYC 1 cut(s) 150
BssSI CACGAG 2 cut(s) 846, 2651
BssT1I CCWWGG 6 cut(s) 183, 577, 1011, 2560, 2674, 2900
Bst2BI CACGAG 2 cut(s) 846, 2651
Bst2UI CCWGG 6 cut(s) 1501, 1515, 1581, 2031, 2085, 2805
Bst4CI ACNGT 7 cut(s) 248, 338, 423, 1365, 1399, 1786, 2815
Bst6I CTCTTC 4 cut(s) 280, 303, 1751, 2064
BstACI GRCGYC 1 cut(s) 150
BstAFI CTTAAG 1 cut(s) 2797
BstAPI GCANNNNNTGC 2 cut(s) 461, 2285
BstBAI YACGTR 2 cut(s) 1346, 2707
BstC8I GCNNGC 6 cut(s) 117, 483, 1898, 1953, 2146, 2178
BstDEI CTNAG 6 cut(s) 125, 840, 1212, 1371, 1892, 2874
BstDSI CCRYGG 3 cut(s) 183, 2560, 2674
BstEII GGTNACC 1 cut(s) 851
BstENI CCTNNNNNAGG 1 cut(s) 1211
BstFNI CGCG 1 cut(s) 2310
BstH2I RGCGCY 1 cut(s) 139
BstHHI GCGC 1 cut(s) 138
BstMAI GTCTC 4 cut(s) 277, 849, 1298, 1775
BstMWI GCNNNNNNNGC 5 cut(s) 185, 461, 1969, 2285, 2551
BstNI CCWGG 6 cut(s) 1501, 1515, 1581, 2031, 2085, 2805
BstNSI RCATGY 1 cut(s) 2390
BstPI GGTNACC 1 cut(s) 851
BstSCI CCNGG 7 cut(s) 642, 1499, 1513, 1579, 2029, 2083, 2803
BstSFI CTRYAG 2 cut(s) 282, 2811
BstUI CGCG 1 cut(s) 2310
BstV1I GCAGC 7 cut(s) 274, 296, 1581, 2095, 2272, 2696, 2824
BstV2I GAAGAC 2 cut(s) 332, 2577
BstX2I RGATCY 3 cut(s) 549, 1819, 2807
BstXI CCANNNNNNTGG 1 cut(s) 1153
BstYI RGATCY 3 cut(s) 549, 1819, 2807
BsuRI GGCC 8 cut(s) 188, 651, 722, 886, 1007, 1393, 1900, 2055
BtgI CCRYGG 3 cut(s) 183, 2560, 2674
BtgZI GCGATG 1 cut(s) 23
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 3 cut(s) 253, 279, 1451
Cac8I GCNNGC 6 cut(s) 117, 483, 1898, 1953, 2146, 2178
CaiI CAGNNNCTG 2 cut(s) 2285, 2819
CciI TCATGA 1 cut(s) 2364
CfoI GCGC 1 cut(s) 138
Cfr10I RCCGGY 1 cut(s) 485
CseI GACGC 2 cut(s) 158, 2316
CsiI ACCWGGT 1 cut(s) 2029
Csp6I GTAC 5 cut(s) 693, 1127, 1477, 1981, 2496
CspCI CAANNNNNGTGG 2 cut(s) 280, 315
CviQI GTAC 5 cut(s) 693, 1127, 1477, 1981, 2496
DdeI CTNAG 6 cut(s) 125, 840, 1212, 1371, 1892, 2874
DrdI GACNNNNNNGTC 2 cut(s) 526, 2599
DseDI GACNNNNNNGTC 2 cut(s) 526, 2599
EaeI YGGCCR 3 cut(s) 186, 649, 1898
Eam1104I CTCTTC 4 cut(s) 280, 303, 1751, 2064
EarI CTCTTC 4 cut(s) 280, 303, 1751, 2064
EciI GGCGGA 3 cut(s) 163, 267, 366
Eco130I CCWWGG 6 cut(s) 183, 577, 1011, 2560, 2674, 2900
Eco31I GGTCTC 1 cut(s) 1298
Eco32I GATATC 1 cut(s) 1702
Eco47I GGWCC 7 cut(s) 106, 581, 639, 930, 1063, 2153, 2204
Eco57I CTGAAG 2 cut(s) 968, 2717
Eco72I CACGTG 2 cut(s) 1346, 2707
Eco88I CYCGRG 1 cut(s) 470
Eco91I GGTNACC 1 cut(s) 851
EcoNI CCTNNNNNAGG 1 cut(s) 1211
EcoO109I RGGNCCY 2 cut(s) 930, 2153
EcoO65I GGTNACC 1 cut(s) 851
EcoRI GAATTC 1 cut(s) 2090
EcoRII CCWGG 6 cut(s) 1499, 1513, 1579, 2029, 2083, 2803
EcoRV GATATC 1 cut(s) 1702
EcoT14I CCWWGG 6 cut(s) 183, 577, 1011, 2560, 2674, 2900
ErhI CCWWGG 6 cut(s) 183, 577, 1011, 2560, 2674, 2900
FalI AAGNNNNNCTT 2 cut(s) 2534, 2566
FaqI GGGAC 7 cut(s) 197, 625, 1627, 2139, 2358, 2499, 2629
FauI CCCGC 5 cut(s) 124, 394, 864, 2303, 2654
FauNDI CATATG 2 cut(s) 1618, 2353
FbaI TGATCA 1 cut(s) 1831
FspBI CTAG 3 cut(s) 699, 1460, 2841
GlaI GCGC 1 cut(s) 137
GsaI CCCAGC 1 cut(s) 1614
GsuI CTGGAG 3 cut(s) 1026, 1288, 2106
HaeII RGCGCY 1 cut(s) 139
HaeIII GGCC 8 cut(s) 188, 651, 722, 886, 1007, 1393, 1900, 2055
HapII CCGG 4 cut(s) 486, 584, 644, 673
HgaI GACGC 2 cut(s) 158, 2316
HhaI GCGC 1 cut(s) 138
Hin1I GRCGYC 1 cut(s) 150
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HindIII AAGCTT 2 cut(s) 479, 704
HpaII CCGG 4 cut(s) 486, 584, 644, 673
HphI GGTGA 8 cut(s) 393, 658, 817, 863, 1397, 1826, 2237, 2243
Hpy166II GTNNAC 4 cut(s) 220, 1164, 1465, 1535
Hpy8I GTNNAC 4 cut(s) 220, 1164, 1465, 1535
Hpy99I CGWCG 3 cut(s) 152, 230, 2310
HpyCH4III ACNGT 7 cut(s) 248, 338, 423, 1365, 1399, 1786, 2815
HpyCH4IV ACGT 2 cut(s) 1345, 2706
HpyF10VI GCNNNNNNNGC 5 cut(s) 185, 461, 1969, 2285, 2551
HpyF3I CTNAG 6 cut(s) 125, 840, 1212, 1371, 1892, 2874
HpySE526I ACGT 2 cut(s) 1345, 2706
Hsp92I GRCGYC 1 cut(s) 150
HspAI GCGC 1 cut(s) 136
Kpn2I TCCGGA 1 cut(s) 583
Ksp22I TGATCA 1 cut(s) 1831
LmnI GCTCC 5 cut(s) 143, 204, 254, 379, 2260
Lsp1109I GCAGC 7 cut(s) 274, 296, 1581, 2095, 2272, 2696, 2824
LweI GCATC 7 cut(s) 304, 361, 1548, 1964, 2095, 2706, 2770
MabI ACCWGGT 1 cut(s) 2029
MaeI CTAG 3 cut(s) 699, 1460, 2841
MaeII ACGT 2 cut(s) 1345, 2706
MaeIII GTNAC 3 cut(s) 631, 851, 1760
MfeI CAATTG 1 cut(s) 2264
MflI RGATCY 3 cut(s) 549, 1819, 2807
MhlI GDGCHC 1 cut(s) 2641
MlsI TGGCCA 2 cut(s) 188, 1900
MluNI TGGCCA 2 cut(s) 188, 1900
MlyI GAGTC 4 cut(s) 1478, 1868, 2521, 2885
MmeI TCCRAC 2 cut(s) 267, 741
Mox20I TGGCCA 2 cut(s) 188, 1900
MroI TCCGGA 1 cut(s) 583
MroXI GAANNNNTTC 1 cut(s) 2301
MscI TGGCCA 2 cut(s) 188, 1900
MseI TTAA 8 cut(s) 708, 756, 1026, 1091, 1796, 2538, 2616, 2798
MslI CAYNNNNRTG 4 cut(s) 2352, 2679, 2705, 2911
Msp20I TGGCCA 2 cut(s) 188, 1900
MspA1I CMGCKG 5 cut(s) 287, 1046, 1169, 1370, 1951
MspCI CTTAAG 1 cut(s) 2797
MspI CCGG 4 cut(s) 486, 584, 644, 673
MspR9I CCNGG 7 cut(s) 644, 1501, 1515, 1581, 2031, 2085, 2805
MunI CAATTG 1 cut(s) 2264
Mva1269I GAATGC 3 cut(s) 784, 1229, 2360
MvaI CCWGG 6 cut(s) 1501, 1515, 1581, 2031, 2085, 2805
MvnI CGCG 1 cut(s) 2310
MwoI GCNNNNNNNGC 5 cut(s) 185, 461, 1969, 2285, 2551
NciI CCSGG 1 cut(s) 644
NcoI CCATGG 3 cut(s) 183, 2560, 2674
NdeI CATATG 2 cut(s) 1618, 2353
NlaIV GGNNCC 9 cut(s) 200, 256, 551, 574, 616, 641, 876, 1065, 2155
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 2 cut(s) 851, 1760
NspI RCATGY 1 cut(s) 2390
PagI TCATGA 1 cut(s) 2364
PcsI WCGNNNNNNNCGW 1 cut(s) 527
PctI GAATGC 3 cut(s) 784, 1229, 2360
PdmI GAANNNNTTC 1 cut(s) 2301
PfeI GAWTC 6 cut(s) 239, 603, 812, 1448, 2656, 2774
PfoI TCCNGGA 1 cut(s) 1513
PleI GAGTC 4 cut(s) 1477, 1867, 2520, 2885
PmaCI CACGTG 2 cut(s) 1346, 2707
PmlI CACGTG 2 cut(s) 1346, 2707
PpsI GAGTC 4 cut(s) 1477, 1867, 2520, 2885
Ppu21I YACGTR 2 cut(s) 1346, 2707
PpuMI RGGWCCY 2 cut(s) 930, 2153
Psp5II RGGWCCY 2 cut(s) 930, 2153
Psp6I CCWGG 6 cut(s) 1499, 1513, 1579, 2029, 2083, 2803
PspCI CACGTG 2 cut(s) 1346, 2707
PspEI GGTNACC 1 cut(s) 851
PspFI CCCAGC 1 cut(s) 1610
PspGI CCWGG 6 cut(s) 1499, 1513, 1579, 2029, 2083, 2803
PspN4I GGNNCC 9 cut(s) 200, 256, 551, 574, 616, 641, 876, 1065, 2155
PspPPI RGGWCCY 2 cut(s) 930, 2153
PstI CTGCAG 1 cut(s) 286
PstNI CAGNNNCTG 2 cut(s) 2285, 2819
PsuI RGATCY 3 cut(s) 549, 1819, 2807
PvuII CAGCTG 4 cut(s) 287, 1046, 1169, 1951
RsaI GTAC 5 cut(s) 694, 1128, 1478, 1982, 2497
RsaNI GTAC 5 cut(s) 693, 1127, 1477, 1981, 2496
RseI CAYNNNNRTG 4 cut(s) 2352, 2679, 2705, 2911
SaqAI TTAA 8 cut(s) 708, 756, 1026, 1091, 1796, 2538, 2616, 2798
ScaI AGTACT 2 cut(s) 694, 2497
SchI GAGTC 4 cut(s) 1478, 1868, 2521, 2885
ScrFI CCNGG 7 cut(s) 644, 1501, 1515, 1581, 2031, 2085, 2805
SduI GDGCHC 1 cut(s) 2641
SexAI ACCWGGT 1 cut(s) 2029
SfaNI GCATC 7 cut(s) 304, 361, 1548, 1964, 2095, 2706, 2770
SfcI CTRYAG 2 cut(s) 282, 2811
SinI GGWCC 7 cut(s) 106, 581, 639, 930, 1063, 2153, 2204
SmiMI CAYNNNNRTG 4 cut(s) 2352, 2679, 2705, 2911
SmlI CTYRAG 2 cut(s) 428, 2797
SmoI CTYRAG 2 cut(s) 428, 2797
SpeI ACTAGT 1 cut(s) 698
SspI AATATT 2 cut(s) 771, 2741
SspMI CTAG 3 cut(s) 699, 1460, 2841
StyD4I CCNGG 7 cut(s) 642, 1499, 1513, 1579, 2029, 2083, 2803
StyI CCWWGG 6 cut(s) 183, 577, 1011, 2560, 2674, 2900
TaaI ACNGT 7 cut(s) 248, 338, 423, 1365, 1399, 1786, 2815
TaiI ACGT 2 cut(s) 1348, 2709
TaqI TCGA 4 cut(s) 9, 1713, 2182, 2305
TaqII GACCGA 1 cut(s) 94
TatI WGTACW 2 cut(s) 692, 2495
TauI GCSGC 4 cut(s) 179, 182, 448, 654
TfiI GAWTC 6 cut(s) 239, 603, 812, 1448, 2656, 2774
Tru1I TTAA 8 cut(s) 708, 756, 1026, 1091, 1796, 2538, 2616, 2798
Tru9I TTAA 8 cut(s) 708, 756, 1026, 1091, 1796, 2538, 2616, 2798
TscAI CASTG 3 cut(s) 253, 286, 1458
TseFI GTSAC 2 cut(s) 851, 1760
TseI GCWGC 7 cut(s) 284, 287, 1594, 2108, 2285, 2684, 2837
Tsp45I GTSAC 2 cut(s) 851, 1760
TspGWI ACGGA 1 cut(s) 1501
TspRI CASTG 3 cut(s) 253, 286, 1458
Vha464I CTTAAG 1 cut(s) 2797
VpaK11BI GGWCC 7 cut(s) 106, 581, 639, 930, 1063, 2153, 2204
XagI CCTNNNNNAGG 1 cut(s) 1211
XapI RAATTY 6 cut(s) 891, 1191, 1927, 2090, 2138, 2869
XceI RCATGY 1 cut(s) 2390
XcmI CCANNNNNNNNNTGG 2 cut(s) 1991, 2070
XmnI GAANNNNTTC 1 cut(s) 2301
XspI CTAG 3 cut(s) 699, 1460, 2841
ZrmI AGTACT 2 cut(s) 694, 2497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.