RchiOBHm_Chr5g0055721

Glutamyl endopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002809
Physical Location & Seq
Forward (+)
59050550 .. 59051664
1115 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33268

Sequence Viewer

Length: 927 bp
ATGAACTTTGATATACTCTTCACACTCTACAAGGTGGTGTACATGGTTTTTGAATTCGATTTGAATTACAATTGTGGGATCCATCAATTGTTGCCGCATGGTACCTTTGGTTCTGAAGTTGAGGTACATGGATTCCCTGAGGGTGCTAAGATCAATTTTGTTACTTGGTCCCCAGATGGCCGCCATTCGGCCTTCACTATCCGATTTGATGATGAAAATAGTAGTAGTCAGCTTAAAGTGTGGGTTGCCCAAGTGGAAACCGGGGTGGCCAGGCCTTTGTTCGAGTCGCATGAATACTGTTTGCATGCGGTTTTTGACAATTTTGTTTGGGTGAACGACTCTTCTTTGTTAGTTTGCACCATTCCTCTGTCCCGTGGAGATCCACCAAAGAAACGTTTGGTGCCTTTTGGGCCTAAAATTCAATCTAACGAGCAGAAAAACATTATTCAGGTTAGGACCTTCCAGGATTTGCTTAAGGATGAATATGATGAAGATTTGTTTGATTACTATGCCACCTCACAGCTTGTTTTAGCTTCTTTGGATGGCATGGTGAAGGAAATTGGTCCACCGGCTGCATATACATCAACGGACCCCTCCCCAGATCACAAATATCTTTTAATTAATTTCCCGAGAAGGTTGAGATGTGGAGCTGATGGGAAGTTTGTTAGAGAAATTTGTGATTTGCCTCTTGCCGAGGACATCCCCATTGCATTCAATAGTGTGCGAAGAGGGATGCGCTCTCTCAACTGGAGAGCGGATAAACCATCAACACTCTACTGGTATGTATATGTAACCCTGATGTCAAACTGCTGGGATATCTTAAATGACATGATTTTGAGATGCTATGTTGTAAAAGATAAGACCATATATTCAATAAGAATTTTCTATATAGGAGTACAACTCATGATTCCTTGTACTAATAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

308

Amino Acids

35.49

Weight (kDa)

5.52

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 101
AccB1I GGYRCC 2 cut(s) 101, 400
AccBSI CCGCTC 1 cut(s) 755
AciI CCGC 4 cut(s) 95, 181, 308, 755
AclI AACGTT 1 cut(s) 394
AclWI GGATC 3 cut(s) 73, 86, 374
AcoI YGGCCR 2 cut(s) 178, 267
AcsI RAATTY 4 cut(s) 53, 417, 672, 879
AcuI CTGAAG 1 cut(s) 135
AfaI GTAC 5 cut(s) 41, 103, 126, 897, 916
AfiI CCNNNNNNNGG 1 cut(s) 187
AflII CTTAAG 1 cut(s) 473
AgsI TTSAA 5 cut(s) 53, 64, 422, 715, 873
AjnI CCWGG 2 cut(s) 269, 462
AluBI AGCT 4 cut(s) 232, 523, 533, 650
AluI AGCT 4 cut(s) 232, 523, 533, 650
AlwI GGATC 3 cut(s) 73, 86, 374
Ama87I CYCGRG 1 cut(s) 628
AoxI GGCC 5 cut(s) 178, 189, 267, 272, 410
ApeKI GCWGC 1 cut(s) 572
ApoI RAATTY 4 cut(s) 53, 417, 672, 879
AseI ATTAAT 1 cut(s) 621
Asp718I GGTACC 1 cut(s) 101
AspLEI GCGC 1 cut(s) 738
AspS9I GGNCC 5 cut(s) 168, 410, 456, 563, 589
AsuC2I CCSGG 1 cut(s) 262
AsuHPI GGTGA 2 cut(s) 343, 562
AvaI CYCGRG 1 cut(s) 628
AvaII GGWCC 4 cut(s) 168, 456, 563, 589
AxyI CCTNAGG 1 cut(s) 138
BalI TGGCCA 1 cut(s) 269
BamHI GGATCC 1 cut(s) 78
BanI GGYRCC 2 cut(s) 101, 400
BarI GAAGNNNNNNTAC 2 cut(s) 108, 140
BbvI GCAGC 1 cut(s) 559
BccI CCATC 5 cut(s) 90, 170, 536, 647, 772
BciT130I CCWGG 2 cut(s) 271, 464
BcnI CCSGG 1 cut(s) 262
BfrI CTTAAG 1 cut(s) 473
BglI GCCNNNNNGGC 1 cut(s) 409
BisI GCNGC 3 cut(s) 95, 181, 573
BlsI GCNGC 3 cut(s) 96, 182, 574
Bme1390I CCNGG 3 cut(s) 262, 271, 464
Bme18I GGWCC 4 cut(s) 168, 456, 563, 589
BmeT110I CYCGRG 1 cut(s) 628
BmgT120I GGNCC 5 cut(s) 168, 410, 456, 563, 589
BmiI GGNNCC 5 cut(s) 80, 103, 170, 402, 591
BmrFI CCNGG 3 cut(s) 262, 271, 464
BmsI GCATC 2 cut(s) 723, 830
BplI GAGNNNNNCTC 2 cut(s) 885, 917
BpmI CTGGAG 1 cut(s) 769
BpuMI CCSGG 1 cut(s) 262
BsaJI CCNNGG 3 cut(s) 261, 373, 693
Bsc4I CCNNNNNNNGG 1 cut(s) 187
Bse118I RCCGGY 1 cut(s) 568
Bse1I ACTGG 2 cut(s) 752, 782
Bse21I CCTNAGG 1 cut(s) 138
Bse3DI GCAATG 1 cut(s) 705
BseBI CCWGG 2 cut(s) 271, 464
BseDI CCNNGG 3 cut(s) 261, 373, 693
BseGI GGATG 4 cut(s) 484, 547, 699, 738
BseLI CCNNNNNNNGG 1 cut(s) 187
BseMI GCAATG 1 cut(s) 705
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 2 cut(s) 752, 782
BseXI GCAGC 1 cut(s) 559
BseYI CCCAGC 1 cut(s) 810
BshFI GGCC 5 cut(s) 180, 191, 269, 274, 412
BshNI GGYRCC 2 cut(s) 101, 400
BsiHKCI CYCGRG 1 cut(s) 628
BsiSI CCGG 2 cut(s) 261, 569
BslFI GGGAC 2 cut(s) 154, 355
BslI CCNNNNNNNGG 1 cut(s) 187
BsmFI GGGAC 2 cut(s) 154, 355
BsmI GAATGC 1 cut(s) 710
BsnI GGCC 5 cut(s) 180, 191, 269, 274, 412
BsoBI CYCGRG 1 cut(s) 628
Bsp1407I TGTACA 1 cut(s) 39
Bsp143I GATC 4 cut(s) 78, 150, 379, 601
BspACI CCGC 4 cut(s) 95, 181, 308, 755
BspANI GGCC 5 cut(s) 180, 191, 269, 274, 412
BspCNI CTCAG 1 cut(s) 130
BspHI TCATGA 1 cut(s) 903
BspLI GGNNCC 5 cut(s) 80, 103, 170, 402, 591
BspPI GGATC 3 cut(s) 73, 86, 374
BspT107I GGYRCC 2 cut(s) 101, 400
BspTI CTTAAG 1 cut(s) 473
BsrBI CCGCTC 1 cut(s) 755
BsrDI GCAATG 1 cut(s) 705
BsrFI RCCGGY 1 cut(s) 568
BsrGI TGTACA 1 cut(s) 39
BsrI ACTGG 2 cut(s) 752, 782
BssAI RCCGGY 1 cut(s) 568
BssECI CCNNGG 3 cut(s) 261, 373, 693
BssMI GATC 4 cut(s) 78, 150, 379, 601
Bst2UI CCWGG 2 cut(s) 271, 464
Bst4CI ACNGT 1 cut(s) 299
Bst6I CTCTTC 3 cut(s) 23, 346, 721
BstAFI CTTAAG 1 cut(s) 473
BstAUI TGTACA 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 2 cut(s) 138, 147
BstDSI CCRYGG 1 cut(s) 373
BstF5I GGATG 4 cut(s) 484, 547, 699, 738
BstHHI GCGC 1 cut(s) 738
BstKTI GATC 4 cut(s) 81, 153, 382, 604
BstMBI GATC 4 cut(s) 78, 150, 379, 601
BstMWI GCNNNNNNNGC 1 cut(s) 409
BstNI CCWGG 2 cut(s) 271, 464
BstNSI RCATGY 1 cut(s) 308
BstSCI CCNGG 3 cut(s) 260, 269, 462
BstV1I GCAGC 1 cut(s) 559
BstX2I RGATCY 2 cut(s) 78, 379
BstYI RGATCY 2 cut(s) 78, 379
Bsu36I CCTNAGG 1 cut(s) 138
BsuRI GGCC 5 cut(s) 180, 191, 269, 274, 412
BtgI CCRYGG 1 cut(s) 373
BtsCI GGATG 4 cut(s) 484, 547, 699, 738
Cac8I GCNNGC 1 cut(s) 306
CciI TCATGA 1 cut(s) 903
CfoI GCGC 1 cut(s) 738
Cfr10I RCCGGY 1 cut(s) 568
Cfr13I GGNCC 5 cut(s) 168, 410, 456, 563, 589
Csp6I GTAC 5 cut(s) 40, 102, 125, 896, 915
CviAII CATG 8 cut(s) 43, 98, 128, 290, 305, 547, 829, 904
CviQI GTAC 5 cut(s) 40, 102, 125, 896, 915
DdeI CTNAG 2 cut(s) 138, 147
DpnI GATC 4 cut(s) 80, 152, 381, 603
DpnII GATC 4 cut(s) 78, 150, 379, 601
EaeI YGGCCR 2 cut(s) 178, 267
Eam1104I CTCTTC 3 cut(s) 23, 346, 721
EarI CTCTTC 3 cut(s) 23, 346, 721
Eco147I AGGCCT 1 cut(s) 274
Eco32I GATATC 1 cut(s) 817
Eco47I GGWCC 4 cut(s) 168, 456, 563, 589
Eco57I CTGAAG 1 cut(s) 135
Eco81I CCTNAGG 1 cut(s) 138
Eco88I CYCGRG 1 cut(s) 628
EcoO109I RGGNCCY 1 cut(s) 456
EcoRI GAATTC 1 cut(s) 53
EcoRII CCWGG 2 cut(s) 269, 462
EcoRV GATATC 1 cut(s) 817
FaeI CATG 8 cut(s) 46, 101, 131, 293, 308, 550, 832, 907
FaqI GGGAC 2 cut(s) 154, 355
FatI CATG 8 cut(s) 42, 97, 127, 289, 304, 546, 828, 903
Fnu4HI GCNGC 3 cut(s) 95, 181, 573
FokI GGATG 4 cut(s) 491, 554, 686, 745
Fsp4HI GCNGC 3 cut(s) 95, 181, 573
GlaI GCGC 1 cut(s) 737
GluI GCNGC 3 cut(s) 95, 181, 573
GsaI CCCAGC 1 cut(s) 814
GsuI CTGGAG 1 cut(s) 769
HaeIII GGCC 5 cut(s) 180, 191, 269, 274, 412
HapII CCGG 2 cut(s) 261, 569
HhaI GCGC 1 cut(s) 738
Hin1II CATG 8 cut(s) 46, 101, 131, 293, 308, 550, 832, 907
Hin6I GCGC 1 cut(s) 736
HinP1I GCGC 1 cut(s) 736
HinfI GANTC 4 cut(s) 132, 284, 338, 907
HpaII CCGG 2 cut(s) 261, 569
HphI GGTGA 2 cut(s) 343, 562
Hpy166II GTNNAC 3 cut(s) 40, 334, 566
Hpy188I TCNGA 2 cut(s) 115, 203
Hpy188III TCNNGA 2 cut(s) 628, 904
Hpy8I GTNNAC 3 cut(s) 40, 334, 566
HpyAV CCTTC 4 cut(s) 202, 469, 547, 627
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4IV ACGT 1 cut(s) 394
HpyCH4V TGCA 4 cut(s) 304, 357, 575, 710
HpyF10VI GCNNNNNNNGC 1 cut(s) 409
HpyF3I CTNAG 2 cut(s) 138, 147
HpySE526I ACGT 1 cut(s) 394
Hsp92II CATG 8 cut(s) 46, 101, 131, 293, 308, 550, 832, 907
HspAI GCGC 1 cut(s) 736
KpnI GGTACC 1 cut(s) 105
Kzo9I GATC 4 cut(s) 78, 150, 379, 601
LmnI GCTCC 1 cut(s) 647
Lsp1109I GCAGC 1 cut(s) 559
LweI GCATC 2 cut(s) 723, 830
MaeII ACGT 1 cut(s) 394
MaeIII GTNAC 2 cut(s) 160, 790
MalI GATC 4 cut(s) 80, 152, 381, 603
MbiI CCGCTC 1 cut(s) 755
MboI GATC 4 cut(s) 78, 150, 379, 601
MboII GAAGA 4 cut(s) 10, 333, 503, 738
MfeI CAATTG 2 cut(s) 70, 86
MflI RGATCY 2 cut(s) 78, 379
MlsI TGGCCA 1 cut(s) 269
MluNI TGGCCA 1 cut(s) 269
MlyI GAGTC 2 cut(s) 293, 332
MnlI CCTC 8 cut(s) 115, 133, 375, 526, 604, 688, 696, 722
Mox20I TGGCCA 1 cut(s) 269
MscI TGGCCA 1 cut(s) 269
MseI TTAA 6 cut(s) 234, 474, 617, 621, 821, 925
Msp20I TGGCCA 1 cut(s) 269
MspCI CTTAAG 1 cut(s) 473
MspI CCGG 2 cut(s) 261, 569
MspR9I CCNGG 3 cut(s) 262, 271, 464
MunI CAATTG 2 cut(s) 70, 86
Mva1269I GAATGC 1 cut(s) 710
MvaI CCWGG 2 cut(s) 271, 464
MwoI GCNNNNNNNGC 1 cut(s) 409
NciI CCSGG 1 cut(s) 262
NdeII GATC 4 cut(s) 78, 150, 379, 601
NlaIII CATG 8 cut(s) 46, 101, 131, 293, 308, 550, 832, 907
NlaIV GGNNCC 5 cut(s) 80, 103, 170, 402, 591
NmeAIII GCCGAG 1 cut(s) 718
NspI RCATGY 1 cut(s) 308
PacI TTAATTAA 1 cut(s) 621
PaeI GCATGC 1 cut(s) 308
PagI TCATGA 1 cut(s) 903
PceI AGGCCT 1 cut(s) 274
PctI GAATGC 1 cut(s) 710
PfeI GAWTC 2 cut(s) 132, 907
PfoI TCCNGGA 1 cut(s) 462
PkrI GCNGC 3 cut(s) 96, 182, 574
PleI GAGTC 2 cut(s) 292, 332
PpsI GAGTC 2 cut(s) 292, 332
PpuMI RGGWCCY 1 cut(s) 456
PshBI ATTAAT 1 cut(s) 621
Psp1406I AACGTT 1 cut(s) 394
Psp5II RGGWCCY 1 cut(s) 456
Psp6I CCWGG 2 cut(s) 269, 462
PspFI CCCAGC 1 cut(s) 810
PspGI CCWGG 2 cut(s) 269, 462
PspN4I GGNNCC 5 cut(s) 80, 103, 170, 402, 591
PspPI GGNCC 5 cut(s) 168, 410, 456, 563, 589
PspPPI RGGWCCY 1 cut(s) 456
PsrI GAACNNNNNNTAC 2 cut(s) 94, 126
PsuI RGATCY 2 cut(s) 78, 379
RsaI GTAC 5 cut(s) 41, 103, 126, 897, 916
RsaNI GTAC 5 cut(s) 40, 102, 125, 896, 915
SaqAI TTAA 6 cut(s) 234, 474, 617, 621, 821, 925
SatI GCNGC 3 cut(s) 95, 181, 573
Sau3AI GATC 4 cut(s) 78, 150, 379, 601
Sau96I GGNCC 5 cut(s) 168, 410, 456, 563, 589
SchI GAGTC 2 cut(s) 293, 332
ScrFI CCNGG 3 cut(s) 262, 271, 464
SfaNI GCATC 2 cut(s) 723, 830
SinI GGWCC 4 cut(s) 168, 456, 563, 589
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
SphI GCATGC 1 cut(s) 308
SseBI AGGCCT 1 cut(s) 274
SsiI CCGC 4 cut(s) 95, 181, 308, 755
StuI AGGCCT 1 cut(s) 274
StyD4I CCNGG 3 cut(s) 260, 269, 462
TaaI ACNGT 1 cut(s) 299
TaiI ACGT 1 cut(s) 397
TaqI TCGA 2 cut(s) 57, 282
TatI WGTACW 3 cut(s) 39, 895, 914
TauI GCSGC 2 cut(s) 97, 183
TfiI GAWTC 2 cut(s) 132, 907
Tru1I TTAA 6 cut(s) 234, 474, 617, 621, 821, 925
Tru9I TTAA 6 cut(s) 234, 474, 617, 621, 821, 925
TseI GCWGC 1 cut(s) 572
TspDTI ATGAA 5 cut(s) 17, 228, 306, 495, 504
TspGWI ACGGA 1 cut(s) 602
Vha464I CTTAAG 1 cut(s) 473
VpaK11BI GGWCC 4 cut(s) 168, 456, 563, 589
VspI ATTAAT 1 cut(s) 621
XapI RAATTY 4 cut(s) 53, 417, 672, 879
XceI RCATGY 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.