RchiOBHm_Chr7g0241431

Glutamyl endopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002809
Physical Location & Seq
Reverse (-)
67528771 .. 67534349
5579 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21634

Sequence Viewer

Length: 2895 bp
ATGAGCGATAGGATGCTCCATCTTCAAAAAGTCTATCATCGCCTATCTCTTCGCTCCTTCACTCCACACTCCCTCCCTTTCAAACCCCCCCAATTCCCATCCCTCCGTTCGCCTCCGCGCCTCCGTGTGCGGCCGCGAAGCCACGCCGTCAGGTCCATCGGAACCGCCGCCGCCGTCATGTCTGCTAGCGCTAGCTCGAGGCTCAGCAACATCGTCCCCCTCAACGCCGTCGTTGCCGAGGACGGCGGCAACGGTTCCGTCTCTTCTGCCACTGCTACTACATTTGAAGATGAAGATTCCTCGCTGGGATATCGTCTTCCTCCGAAGGAAATTAAAGATATAGTTGATGCTCCGCCGCTTCCGGCATTGTCATTCTCGCCGTACAGGGATAAGATACTGTTTCTGAAGCGGAGAGCTTTACCGCCGTTGACAGAACTTGCAAGACCAGAGGAGAAGCTTGCTGGTGTTCGGATTGATGGGAAATGCAATACAAGGACTCGGATGTCGTTTTATACTGGGATTGGGATCCATCAATTGCTGCCGGATGGTACTCTTGGTTCCGAAGTTGAGGTGCATGGATTCCCTGAGGGTGCTAAGATCAATTTTGTTACTTGGTCACCAGATGGCCGCCATTTGGCCTTCACTATTCGATTTGATGATGAAGATACTAGTAGTAAGCTTAAAGTGTGGGTTGCCCAAGTGGAAACCGGGATAGCCAGGCCTTTGTTTGAGTCACATGAATACTGTTTGAATGCGGTTTTTGACAATTTTGTTTGGGTGAACGACTCTTCTTTGTTAGTTTGCACCATTCCTCTGTCCCGTGGAGACCCGCCAAAGAAACCTTTGGTGCCTTTTGGACCTAAAATTCAATCTAACGAACGGAAAAACATTATTCAAGTTAGAACCTTCCAGGATTTGCTGAAGGATGAGTATGATGAAGATTTGTTTGATTACTATGCCACCTCACAGCTTGTTTTAGCTTCTTTGGATGGTATAGTGAAGGAAATTGGTCCACTGGCTGTATATACCTCAATGGACCCCTCTCCTGATCACAAATACCTTTTAATTAGTTCGCTTCACAGACCATACTCTTTCATTGTACCATGCGGAAGATTTCCCAAGAAGGTTGAGATGTGGACAGCTGATGGGAAGTTTGTTAGAACTCTTTGTGATTTGCCTCTTGCTGAGGACATCCCCATTGCATTCAATAGTGTGCGAAGAGGGATGCGCTCTCTCAACTGGAGAGCGGATAAACCATCAACACTCTACTGGGTTGAGACCCAAGATGAAGGAGATGCGAAAGTGGAAGTTTCTCCACGTGATATTGTTCATACACAGCCTGCTGAACCACTAGAAGGTGAAGATCCAACAATCTTACACAAACTCGATCTCCGTTATGGAGGGATTTCTTGGTCCGATGATTCACTAGCTCTAGTTTACGAGTCATGGTACAAAACACGGAGAACAAGAACCTGGGTAATTTCTCCTGGATCAAATGATGTCAGTCCCCGCATCCTGTTTGATAGGTCATCGGAAGATGTGTACTCTGATCCTGGCTCCCCCATGCTGCGGAGAACTCCTGCTGGCACATATGTGCTTGCAAAAGTGAAGAAAGAAAATGATGAAGGCACTTATTTACTACTGAATGGAAATGGTGCTACACCAGAGGGGAATATCCCGTTCCTTGATTTGTTTGACATAAATACAGGAAATAAAGAACGAATATGGAAGAGTGACAAAGAAAAGTATTATGAGGGTGTTGTTGCTTTAATGTCTGATGAGAAAGAAGGAGATCTTCCCATCAATACCTTGAAAATATTGACTTCCAAGGAGTCAAAAACTGAAAACACCCAATATTATATCCTAAGCTGGCCGGAAAAGAAAGCATGTCAAATTACAAATTTTCCTCATCCGTATCCACAGCTGGCATCATTGCAGAAAGAGATGGTCAGGTACCAGAGAAAGGATGGGGTTCAACTAACCGCAACACTATATCTTCCACCAGGCTACGATCCATCCAGAGATGGCCCTCTTCCATGTTTGGTTTGGTCTTACCCTGGAGAATTCAAAAGCAAAGATGCTGCCGGACAAGTTCGTGGTTCTCCTAATGAATTTGCTGGGATAGGTCCAACATCAGCCTTGCTCTGGATGGCTCGAAGGTTTGCTATTTTATCTGGACCAACTATTCCTATAATTGGTGAGGGTGATGATGAAGCAAATGATAGGTATGTGGAGCAATTGGTTGCAAGTGCAGAGGCTGCTGTGGAGGAAGTCATTCGACGTGGAGTGGCTCATCCAAAGAAAATTGCTGTTGGGGGACATTCATATGGGGCATTCATGACTGCAAACCTACTGGCACATGCTCCTCATCTTTTTGCTTGTGGAATTGCTCGTTCGGGTGCTTACAACAGAACACTTACTCCTTTCGGTTTTCAGAATGAGGAGAGAACTCTTTGGGAGGCTACTAGTACTTATGTGGAGATGAGTCCTTTCATGTCAGCTAATAAAATTAAGAAGCCAATCTTGCTTATCCATGGAGAGGAAGACAATAATCCAGGGACTTTAACCATGCAGTCCGATCGTTTCTTTAATGCTCTGAAAGGTCATGGTGCTCTTTGCCGCCTTGTGATTCTTCCCTTTGAAAGCCATGGCTATGCTGCAAGAGAGAGTATCATGCATGTGCTGTGGGAAACTGACAGGTGGTTGCAAAAATATTGTGTGTCAGACACTTCTGATGTAAATGTTAATCAAGATGCATGTAAAGACAATATAGGCAGAGGATCTACAAATTCTGAAAGCAAAGCAGTAGCTACTGGCGGAGGCAGTGGCTCAGAAGTGTCAAATTCTGAGCATGAAGGAGTTGACTCTTTGCCAAGATCATTAATACATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

964

Amino Acids

107.33

Weight (kDa)

6.14

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 752 - 905 2.2e-22 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 502
Acc65I GGTACC 1 cut(s) 1953
AccB1I GGYRCC 2 cut(s) 847, 1953
AccBSI CCGCTC 1 cut(s) 1247
AccII CGCG 2 cut(s) 118, 136
AclWI GGATC 7 cut(s) 520, 533, 1358, 1498, 1544, 2006, 2788
AcoI YGGCCR 3 cut(s) 131, 625, 1871
AcsI RAATTY 6 cut(s) 864, 1900, 2063, 2111, 2788, 2842
AcuI CTGAAG 2 cut(s) 425, 941
AcvI CACGTG 1 cut(s) 1319
AfaI GTAC 7 cut(s) 383, 550, 1101, 1451, 1544, 1955, 2470
AfeI AGCGCT 1 cut(s) 190
AfiI CCNNNNNNNGG 7 cut(s) 634, 1355, 1569, 1963, 2145, 2195, 2539
AhlI ACTAGT 2 cut(s) 668, 2465
AjiI CACGTC 1 cut(s) 2282
AjnI CCWGG 8 cut(s) 716, 909, 1472, 1486, 1552, 2002, 2056, 2554
AleI CACNNNNGTG 1 cut(s) 1592
AloI GAACNNNNNNTCC 2 cut(s) 2404, 2436
Alw21I GWGCWC 1 cut(s) 2614
Alw26I GTCTC 3 cut(s) 265, 819, 1271
AlwI GGATC 7 cut(s) 520, 533, 1358, 1498, 1544, 2006, 2788
AlwNI CAGNNNCTG 1 cut(s) 2258
Ama87I CYCGRG 1 cut(s) 196
Aor51HI AGCGCT 1 cut(s) 190
AoxI GGCC 6 cut(s) 131, 625, 636, 719, 1871, 2026
ApeKI GCWGC 5 cut(s) 538, 1567, 2081, 2258, 2657
ApoI RAATTY 6 cut(s) 864, 1900, 2063, 2111, 2788, 2842
AseI ATTAAT 1 cut(s) 2882
Asp700I GAANNNNTTC 1 cut(s) 2274
Asp718I GGTACC 1 cut(s) 1953
AspLEI GCGC 3 cut(s) 120, 191, 1230
AspS9I GGNCC 8 cut(s) 153, 857, 1010, 1036, 1413, 2027, 2126, 2177
AsuC2I CCSGG 1 cut(s) 709
AsuHPI GGTGA 5 cut(s) 609, 790, 1370, 2210, 2216
AsuNHI GCTAGC 2 cut(s) 185, 191
AvaI CYCGRG 1 cut(s) 196
AvaII GGWCC 7 cut(s) 153, 857, 1010, 1036, 1413, 2126, 2177
AxyI CCTNAGG 1 cut(s) 585
BamHI GGATCC 1 cut(s) 525
BanI GGYRCC 2 cut(s) 847, 1953
BbrPI CACGTG 1 cut(s) 1319
BbsI GAAGAC 2 cut(s) 308, 2550
Bbv12I GWGCWC 1 cut(s) 2614
BbvCI CCTCAGC 1 cut(s) 1185
BbvI GCAGC 5 cut(s) 525, 1554, 2068, 2245, 2644
BceAI ACGGC 6 cut(s) 131, 158, 212, 259, 364, 409
BcgI CGANNNNNNTGC 2 cut(s) 2561, 2595
BciT130I CCWGG 8 cut(s) 718, 911, 1474, 1488, 1554, 2004, 2058, 2556
BciVI GTATCC 1 cut(s) 1926
BclI TGATCA 1 cut(s) 1048
BcnI CCSGG 1 cut(s) 709
BcoDI GTCTC 3 cut(s) 265, 819, 1271
BcuI ACTAGT 2 cut(s) 668, 2465
BfaI CTAG 7 cut(s) 186, 192, 669, 1352, 1427, 1433, 2466
BfoI RGCGCY 1 cut(s) 192
BfuI GTATCC 1 cut(s) 1926
BglII AGATCT 1 cut(s) 1792
BlpI GCTNAGC 1 cut(s) 203
BmcAI AGTACT 1 cut(s) 2470
Bme1390I CCNGG 9 cut(s) 709, 718, 911, 1474, 1488, 1554, 2004, 2058, 2556
Bme18I GGWCC 7 cut(s) 153, 857, 1010, 1036, 1413, 2126, 2177
BmeT110I CYCGRG 1 cut(s) 196
BmgBI CACGTC 1 cut(s) 2282
BmgT120I GGNCC 8 cut(s) 153, 857, 1010, 1036, 1413, 2027, 2126, 2177
BmiI GGNNCC 8 cut(s) 163, 256, 527, 559, 849, 1038, 1558, 1955
BmrFI CCNGG 9 cut(s) 709, 718, 911, 1474, 1488, 1554, 2004, 2058, 2556
BmrI ACTGGG 2 cut(s) 525, 1279
BmsI GCATC 8 cut(s) 3, 337, 1215, 1285, 1521, 1937, 2068, 2743
BmtI GCTAGC 2 cut(s) 189, 195
BmuI ACTGGG 2 cut(s) 525, 1279
BpiI GAAGAC 2 cut(s) 308, 2550
BpmI CTGGAG 2 cut(s) 1261, 2079
Bpu10I CCTNAGC 2 cut(s) 1185, 1865
Bpu1102I GCTNAGC 1 cut(s) 203
BpuMI CCSGG 1 cut(s) 709
BsaAI YACGTR 1 cut(s) 1319
BsaBI GATNNNNATC 1 cut(s) 524
BsaI GGTCTC 2 cut(s) 819, 1271
BsaJI CCNNGG 8 cut(s) 237, 820, 1473, 1827, 2056, 2533, 2555, 2647
BsaXI ACNNNNNCTCC 6 cut(s) 57, 87, 1374, 1404, 2404, 2434
Bsc4I CCNNNNNNNGG 7 cut(s) 634, 1355, 1569, 1963, 2145, 2195, 2539
Bse1I ACTGG 6 cut(s) 520, 1020, 1244, 1274, 2358, 2818
Bse21I CCTNAGG 1 cut(s) 585
Bse3DI GCAATG 3 cut(s) 1197, 1931, 2887
Bse8I GATNNNNATC 1 cut(s) 524
BseBI CCWGG 8 cut(s) 718, 911, 1474, 1488, 1554, 2004, 2058, 2556
BseDI CCNNGG 8 cut(s) 237, 820, 1473, 1827, 2056, 2533, 2555, 2647
BseJI GATNNNNATC 1 cut(s) 524
BseLI CCNNNNNNNGG 7 cut(s) 634, 1355, 1569, 1963, 2145, 2195, 2539
BseMI GCAATG 3 cut(s) 1197, 1931, 2887
BseMII CTCAG 5 cut(s) 217, 576, 1176, 2838, 2844
BseNI ACTGG 6 cut(s) 520, 1020, 1244, 1274, 2358, 2818
BseRI GAGGAG 3 cut(s) 464, 2355, 2456
BseX3I CGGCCG 1 cut(s) 131
BseXI GCAGC 5 cut(s) 525, 1554, 2068, 2245, 2644
BseYI CCCAGC 2 cut(s) 304, 2117
BsgI GTGCAG 1 cut(s) 2271
Bsh1236I CGCG 2 cut(s) 118, 136
Bsh1285I CGRYCG 2 cut(s) 134, 2581
BshFI GGCC 6 cut(s) 133, 627, 638, 721, 1873, 2028
BshNI GGYRCC 2 cut(s) 847, 1953
BsiEI CGRYCG 2 cut(s) 134, 2581
BsiHKAI GWGCWC 1 cut(s) 2614
BsiHKCI CYCGRG 1 cut(s) 196
BsiSI CCGG 5 cut(s) 362, 542, 708, 1874, 2085
BslFI GGGAC 5 cut(s) 200, 802, 1491, 2331, 2572
BslI CCNNNNNNNGG 7 cut(s) 634, 1355, 1569, 1963, 2145, 2195, 2539
BsmAI GTCTC 3 cut(s) 265, 819, 1271
BsmBI CGTCTC 1 cut(s) 265
BsmFI GGGAC 5 cut(s) 200, 802, 1491, 2331, 2572
BsmI GAATGC 3 cut(s) 757, 1202, 2333
BsnI GGCC 6 cut(s) 133, 627, 638, 721, 1873, 2028
Bso31I GGTCTC 2 cut(s) 819, 1271
BsoBI CYCGRG 1 cut(s) 196
Bsp1286I GDGCHC 1 cut(s) 2614
Bsp1720I GCTNAGC 1 cut(s) 203
Bsp19I CCATGG 2 cut(s) 2533, 2647
BspANI GGCC 6 cut(s) 133, 627, 638, 721, 1873, 2028
BspCNI CTCAG 5 cut(s) 216, 577, 1177, 2839, 2843
BspFNI CGCG 2 cut(s) 118, 136
BspHI TCATGA 1 cut(s) 2337
BspLI GGNNCC 8 cut(s) 163, 256, 527, 559, 849, 1038, 1558, 1955
BspOI GCTAGC 2 cut(s) 189, 195
BspPI GGATC 7 cut(s) 520, 533, 1358, 1498, 1544, 2006, 2788
BspT107I GGYRCC 2 cut(s) 847, 1953
BspTNI GGTCTC 2 cut(s) 819, 1271
BsrBI CCGCTC 1 cut(s) 1247
BsrDI GCAATG 3 cut(s) 1197, 1931, 2887
BsrI ACTGG 6 cut(s) 520, 1020, 1244, 1274, 2358, 2818
BssECI CCNNGG 8 cut(s) 237, 820, 1473, 1827, 2056, 2533, 2555, 2647
BssT1I CCWWGG 3 cut(s) 1827, 2533, 2647
Bst2UI CCWGG 8 cut(s) 718, 911, 1474, 1488, 1554, 2004, 2058, 2556
Bst4CI ACNGT 3 cut(s) 254, 399, 746
Bst6I CTCTTC 6 cut(s) 54, 268, 793, 1213, 1724, 2037
BstAPI GCANNNNNTGC 1 cut(s) 2258
BstBAI YACGTR 1 cut(s) 1319
BstC8I GCNNGC 8 cut(s) 187, 193, 459, 1341, 1585, 1599, 1871, 1926
BstDEI CTNAG 7 cut(s) 203, 585, 594, 1185, 1865, 2830, 2847
BstDSI CCRYGG 3 cut(s) 820, 2533, 2647
BstEII GGTNACC 1 cut(s) 615
BstFNI CGCG 2 cut(s) 118, 136
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 3 cut(s) 120, 191, 1230
BstMAI GTCTC 3 cut(s) 265, 819, 1271
BstMCI CGRYCG 2 cut(s) 134, 2581
BstMWI GCNNNNNNNGC 3 cut(s) 233, 2258, 2524
BstNI CCWGG 8 cut(s) 718, 911, 1474, 1488, 1554, 2004, 2058, 2556
BstNSI RCATGY 4 cut(s) 1890, 2363, 2681, 2760
BstPI GGTNACC 1 cut(s) 615
BstSCI CCNGG 9 cut(s) 707, 716, 909, 1472, 1486, 1552, 2002, 2056, 2554
BstUI CGCG 2 cut(s) 118, 136
BstV1I GCAGC 5 cut(s) 525, 1554, 2068, 2245, 2644
BstV2I GAAGAC 2 cut(s) 308, 2550
BstX2I RGATCY 4 cut(s) 525, 1363, 1792, 2780
BstYI RGATCY 4 cut(s) 525, 1363, 1792, 2780
BstZI CGGCCG 1 cut(s) 131
Bsu36I CCTNAGG 1 cut(s) 585
BsuI GTATCC 1 cut(s) 1926
BsuRI GGCC 6 cut(s) 133, 627, 638, 721, 1873, 2028
BtgI CCRYGG 3 cut(s) 820, 2533, 2647
BtgZI GCGATG 1 cut(s) 23
BtrI CACGTC 1 cut(s) 2282
BtsI GCAGTG 2 cut(s) 270, 2830
BtsIMutI CAGTG 3 cut(s) 270, 1013, 2830
Cac8I GCNNGC 8 cut(s) 187, 193, 459, 1341, 1585, 1599, 1871, 1926
CaiI CAGNNNCTG 1 cut(s) 2258
CciI TCATGA 1 cut(s) 2337
CciNI GCGGCCGC 1 cut(s) 131
CfoI GCGC 3 cut(s) 120, 191, 1230
Cfr13I GGNCC 8 cut(s) 153, 857, 1010, 1036, 1413, 2027, 2126, 2177
Csp6I GTAC 7 cut(s) 382, 549, 1100, 1450, 1543, 1954, 2469
CviQI GTAC 7 cut(s) 382, 549, 1100, 1450, 1543, 1954, 2469
DdeI CTNAG 7 cut(s) 203, 585, 594, 1185, 1865, 2830, 2847
DrdI GACNNNNNNGTC 1 cut(s) 502
DseDI GACNNNNNNGTC 1 cut(s) 502
EaeI YGGCCR 3 cut(s) 131, 625, 1871
EagI CGGCCG 1 cut(s) 131
Eam1104I CTCTTC 6 cut(s) 54, 268, 793, 1213, 1724, 2037
EarI CTCTTC 6 cut(s) 54, 268, 793, 1213, 1724, 2037
EciI GGCGGA 2 cut(s) 342, 2832
EclXI CGGCCG 1 cut(s) 131
Eco130I CCWWGG 3 cut(s) 1827, 2533, 2647
Eco147I AGGCCT 1 cut(s) 721
Eco31I GGTCTC 2 cut(s) 819, 1271
Eco32I GATATC 1 cut(s) 311
Eco47I GGWCC 7 cut(s) 153, 857, 1010, 1036, 1413, 2126, 2177
Eco47III AGCGCT 1 cut(s) 190
Eco52I CGGCCG 1 cut(s) 131
Eco57I CTGAAG 2 cut(s) 425, 941
Eco72I CACGTG 1 cut(s) 1319
Eco81I CCTNAGG 1 cut(s) 585
Eco88I CYCGRG 1 cut(s) 196
Eco91I GGTNACC 1 cut(s) 615
EcoO65I GGTNACC 1 cut(s) 615
EcoRI GAATTC 1 cut(s) 2063
EcoRII CCWGG 8 cut(s) 716, 909, 1472, 1486, 1552, 2002, 2056, 2554
EcoRV GATATC 1 cut(s) 311
EcoT14I CCWWGG 3 cut(s) 1827, 2533, 2647
EcoT22I ATGCAT 2 cut(s) 2679, 2758
ErhI CCWWGG 3 cut(s) 1827, 2533, 2647
Esp3I CGTCTC 1 cut(s) 265
FalI AAGNNNNNCTT 4 cut(s) 1779, 1811, 2507, 2539
FaqI GGGAC 5 cut(s) 200, 802, 1491, 2331, 2572
FauI CCCGC 2 cut(s) 837, 1517
FauNDI CATATG 2 cut(s) 1591, 2326
FbaI TGATCA 1 cut(s) 1048
FspBI CTAG 7 cut(s) 186, 192, 669, 1352, 1427, 1433, 2466
GlaI GCGC 3 cut(s) 119, 190, 1229
GsaI CCCAGC 2 cut(s) 308, 2121
GsuI CTGGAG 2 cut(s) 1261, 2079
HaeII RGCGCY 1 cut(s) 192
HaeIII GGCC 6 cut(s) 133, 627, 638, 721, 1873, 2028
HapII CCGG 5 cut(s) 362, 542, 708, 1874, 2085
HhaI GCGC 3 cut(s) 120, 191, 1230
Hin6I GCGC 3 cut(s) 118, 189, 1228
HinP1I GCGC 3 cut(s) 118, 189, 1228
HincII GTYRAC 2 cut(s) 429, 2863
HindII GTYRAC 2 cut(s) 429, 2863
HindIII AAGCTT 2 cut(s) 455, 677
HpaII CCGG 5 cut(s) 362, 542, 708, 1874, 2085
HphI GGTGA 5 cut(s) 609, 790, 1370, 2210, 2216
Hpy166II GTNNAC 7 cut(s) 429, 781, 1013, 1137, 1438, 1543, 2863
Hpy188III TCNNGA 6 cut(s) 1046, 2019, 2146, 2175, 2338, 2750
Hpy8I GTNNAC 7 cut(s) 429, 781, 1013, 1137, 1438, 1543, 2863
Hpy99I CGWCG 2 cut(s) 233, 2283
HpyCH4III ACNGT 3 cut(s) 254, 399, 746
HpyCH4IV ACGT 2 cut(s) 1318, 2281
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 2258, 2524
HpyF3I CTNAG 7 cut(s) 203, 585, 594, 1185, 1865, 2830, 2847
HpySE526I ACGT 2 cut(s) 1318, 2281
HspAI GCGC 3 cut(s) 118, 189, 1228
KpnI GGTACC 1 cut(s) 1957
Ksp22I TGATCA 1 cut(s) 1048
LmnI GCTCC 6 cut(s) 21, 59, 355, 1562, 2233, 2368
Lsp1109I GCAGC 5 cut(s) 525, 1554, 2068, 2245, 2644
LweI GCATC 8 cut(s) 3, 337, 1215, 1285, 1521, 1937, 2068, 2743
MaeI CTAG 7 cut(s) 186, 192, 669, 1352, 1427, 1433, 2466
MaeII ACGT 2 cut(s) 1318, 2281
MaeIII GTNAC 4 cut(s) 607, 615, 732, 1733
MbiI CCGCTC 1 cut(s) 1247
MfeI CAATTG 2 cut(s) 533, 2237
MflI RGATCY 4 cut(s) 525, 1363, 1792, 2780
MhlI GDGCHC 1 cut(s) 2614
MlyI GAGTC 7 cut(s) 490, 740, 779, 1451, 1841, 2494, 2858
MmeI TCCRAC 2 cut(s) 1391, 2153
Mph1103I ATGCAT 2 cut(s) 2679, 2758
MroXI GAANNNNTTC 1 cut(s) 2274
MseI TTAA 9 cut(s) 333, 681, 1064, 1769, 2511, 2564, 2589, 2745, 2882
MslI CAYNNNNRTG 4 cut(s) 1592, 2325, 2652, 2678
MspA1I CMGCKG 2 cut(s) 1142, 1924
MspI CCGG 5 cut(s) 362, 542, 708, 1874, 2085
MspR9I CCNGG 9 cut(s) 709, 718, 911, 1474, 1488, 1554, 2004, 2058, 2556
MunI CAATTG 2 cut(s) 533, 2237
Mva1269I GAATGC 3 cut(s) 757, 1202, 2333
MvaI CCWGG 8 cut(s) 718, 911, 1474, 1488, 1554, 2004, 2058, 2556
MvnI CGCG 2 cut(s) 118, 136
MwoI GCNNNNNNNGC 3 cut(s) 233, 2258, 2524
NciI CCSGG 1 cut(s) 709
NcoI CCATGG 2 cut(s) 2533, 2647
NdeI CATATG 2 cut(s) 1591, 2326
NheI GCTAGC 2 cut(s) 185, 191
NlaIV GGNNCC 8 cut(s) 163, 256, 527, 559, 849, 1038, 1558, 1955
NmeAIII GCCGAG 1 cut(s) 262
NmuCI GTSAC 3 cut(s) 615, 732, 1733
NotI GCGGCCGC 1 cut(s) 131
NsiI ATGCAT 2 cut(s) 2679, 2758
NspI RCATGY 4 cut(s) 1890, 2363, 2681, 2760
OliI CACNNNNGTG 1 cut(s) 1592
PaeR7I CTCGAG 1 cut(s) 196
PagI TCATGA 1 cut(s) 2337
PceI AGGCCT 1 cut(s) 721
PctI GAATGC 3 cut(s) 757, 1202, 2333
PdmI GAANNNNTTC 1 cut(s) 2274
PfeI GAWTC 4 cut(s) 296, 579, 1421, 2629
PfoI TCCNGGA 2 cut(s) 909, 1486
Ple19I CGATCG 1 cut(s) 2581
PleI GAGTC 7 cut(s) 490, 739, 779, 1450, 1840, 2493, 2858
PmaCI CACGTG 1 cut(s) 1319
PmlI CACGTG 1 cut(s) 1319
PpsI GAGTC 7 cut(s) 490, 739, 779, 1450, 1840, 2493, 2858
Ppu21I YACGTR 1 cut(s) 1319
PshBI ATTAAT 1 cut(s) 2882
Psp6I CCWGG 8 cut(s) 716, 909, 1472, 1486, 1552, 2002, 2056, 2554
PspCI CACGTG 1 cut(s) 1319
PspEI GGTNACC 1 cut(s) 615
PspFI CCCAGC 2 cut(s) 304, 2117
PspGI CCWGG 8 cut(s) 716, 909, 1472, 1486, 1552, 2002, 2056, 2554
PspN4I GGNNCC 8 cut(s) 163, 256, 527, 559, 849, 1038, 1558, 1955
PspPI GGNCC 8 cut(s) 153, 857, 1010, 1036, 1413, 2027, 2126, 2177
PspXI VCTCGAGB 1 cut(s) 196
PsrI GAACNNNNNNTAC 2 cut(s) 541, 573
PstNI CAGNNNCTG 1 cut(s) 2258
PsuI RGATCY 4 cut(s) 525, 1363, 1792, 2780
PvuI CGATCG 1 cut(s) 2581
PvuII CAGCTG 2 cut(s) 1142, 1924
RsaI GTAC 7 cut(s) 383, 550, 1101, 1451, 1544, 1955, 2470
RsaNI GTAC 7 cut(s) 382, 549, 1100, 1450, 1543, 1954, 2469
RseI CAYNNNNRTG 4 cut(s) 1592, 2325, 2652, 2678
SaqAI TTAA 9 cut(s) 333, 681, 1064, 1769, 2511, 2564, 2589, 2745, 2882
Sau96I GGNCC 8 cut(s) 153, 857, 1010, 1036, 1413, 2027, 2126, 2177
ScaI AGTACT 1 cut(s) 2470
SchI GAGTC 7 cut(s) 490, 740, 779, 1451, 1841, 2494, 2858
ScrFI CCNGG 9 cut(s) 709, 718, 911, 1474, 1488, 1554, 2004, 2058, 2556
SduI GDGCHC 1 cut(s) 2614
SfaNI GCATC 8 cut(s) 3, 337, 1215, 1285, 1521, 1937, 2068, 2743
Sfr274I CTCGAG 1 cut(s) 196
SinI GGWCC 7 cut(s) 153, 857, 1010, 1036, 1413, 2126, 2177
SlaI CTCGAG 1 cut(s) 196
SmiMI CAYNNNNRTG 4 cut(s) 1592, 2325, 2652, 2678
SmlI CTYRAG 1 cut(s) 196
SmoI CTYRAG 1 cut(s) 196
SpeI ACTAGT 2 cut(s) 668, 2465
SseBI AGGCCT 1 cut(s) 721
SspI AATATT 3 cut(s) 1818, 1856, 2714
SspMI CTAG 7 cut(s) 186, 192, 669, 1352, 1427, 1433, 2466
StuI AGGCCT 1 cut(s) 721
StyD4I CCNGG 9 cut(s) 707, 716, 909, 1472, 1486, 1552, 2002, 2056, 2554
StyI CCWWGG 3 cut(s) 1827, 2533, 2647
TaaI ACNGT 3 cut(s) 254, 399, 746
TaiI ACGT 2 cut(s) 1321, 2284
TaqI TCGA 5 cut(s) 197, 649, 1386, 2155, 2278
TatI WGTACW 2 cut(s) 1542, 2468
TauI GCSGC 8 cut(s) 133, 136, 170, 173, 249, 358, 630, 2622
TfiI GAWTC 4 cut(s) 296, 579, 1421, 2629
Tru1I TTAA 9 cut(s) 333, 681, 1064, 1769, 2511, 2564, 2589, 2745, 2882
Tru9I TTAA 9 cut(s) 333, 681, 1064, 1769, 2511, 2564, 2589, 2745, 2882
TscAI CASTG 3 cut(s) 277, 1020, 2830
TseFI GTSAC 3 cut(s) 615, 732, 1733
TseI GCWGC 5 cut(s) 538, 1567, 2081, 2258, 2657
Tsp45I GTSAC 3 cut(s) 615, 732, 1733
TspGWI ACGGA 7 cut(s) 95, 113, 247, 895, 1382, 1474, 1902
TspRI CASTG 3 cut(s) 277, 1020, 2830
VpaK11BI GGWCC 7 cut(s) 153, 857, 1010, 1036, 1413, 2126, 2177
VspI ATTAAT 1 cut(s) 2882
XapI RAATTY 6 cut(s) 864, 1900, 2063, 2111, 2788, 2842
XceI RCATGY 4 cut(s) 1890, 2363, 2681, 2760
XcmI CCANNNNNNNNNTGG 2 cut(s) 1964, 2043
XhoI CTCGAG 1 cut(s) 196
XmnI GAANNNNTTC 1 cut(s) 2274
XspI CTAG 7 cut(s) 186, 192, 669, 1352, 1427, 1433, 2466
ZrmI AGTACT 1 cut(s) 2470
Zsp2I ATGCAT 2 cut(s) 2679, 2758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.