MD00G1179200.v1.1

Glutamyl endopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002809
Physical Location & Seq
Forward (+)
42089421 .. 42092769
3349 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1179200.v1.1.491

Sequence Viewer

Length: 1617 bp
ATGCCCAAGCCTAACCCATGGTCTACATATGACTTGGTTGAGACCCAAGATGAAGGAGATGCAAAAGTGGAAGTTTCTCCACGTGATATAATTTATACACAGCCCGCTGATCCACTAGAAGGTGAAGGGCCAACTGTCTTGCACAAACTTGATCTTCGCTTTGGAGGAATTTCTTGGTCTGATGATTCACTAGCTCTGGTTTACGAGTCTTGGTACAAAACACGGAGAACAAGAACCTGGGTGATTTCTCCTGGATCAAATGATGTCAGTCCGCGCATCCTGTTTGATAGATCGTCAGAAGATGTATATTCCGATCCTGGCTCTCCTATGCTGCGGAGAACTCCTGCTGGGACATATGTGTTAGCAAAAGTAAAGAAGGAAAACGAGGAAGGCACTTATTTACTGCTGAATGGAAATGGTGCCACACCAGAGGGAAATATCCCATTCCTCGACTTGTTTGACACAAATACAGGAAACAAAGAAAGAATATGGAAGAGTGACAAAGAAAAGTATTACGAGACTGTTGTTGCTTTAATGTCTGATGAAAAAGAAGGGGATCTGCCCATTGATCACTTGAAAATATTGACTTCTAAAGAGTCAAAAACTGAAAACACTCAGTATTCTATCATAAGCTGGCCAGAAAAGAAAGCAGTCCAAATTACAGATTTTCCTCATCCATATCCACAGCTGGCATCATTGCAGAAAGAGCTGGTCAAGTACCAGAGGAAGGATGGGGTTCAACTAACTGCAACGCTATACCTTCCACCAGGATATGATCCATCCAGAGATGGCCCTCTTCCGTGTCTGTTCTGGTCTTATCCTGGAGAGTTCAAAAGCAAAGATGCTGCTGGACAAGTTCGTGGTTCTCCTAATGAATTTGCTGGCATAGGTCCAACTTCGGCCCTCCTCTGGCTGGCTCGAAGGTTTGCTATTCTGTCTGGACCAACAATTCCTATTATTGGTGAGGGTGATGTAGAAGCAAATGATAGCTATGTGGAGCAATTGGTTGCAAGCGCAGAGGCTGCTGTGGAGGAAGTTGTTCGACGAGGAGTGGCTCATCCAGACAAAATTGCTGTTGGGGGACATTCATATGGGGCATTCATGACTGCAAACCTCTTGGCACATGCTCCCCATCTTTTCTCTTGTGGAATTGCACGTTCTGGTGCTTACAATAGAACACTTACTCCTTTTGGTTTTCAGAATGAGGATAGAACTCTTTGGGAGGCTACTAGTACTTATGTAGAGATGAGTCCTTTCATGTCCGCTAATAAAATTAAGAAGCCAATCTTGCTCATCCATGGAGAAGAAGACAGCAATTCTGGAACTTTAACCATGCAGTCGGATCGTTTCTTTAATGCTCTGAAAGGTCATGGCGCTCTGTCCCGCCTTGTGATTCTTCCATTTGAGAGTCATGGCTATGCTGCAAGAGAGAGCATCATGCACGTGCTATGGGAAAGTGATAGATGGCTTCAGAAATATTGCGTGTCAGACACTTCCAATGTAAATGCTGATTCTGATGCAAGTGAAAAAGAGAACTTAGGCACAGGTTCTACAGCTTCTGAAAGCAAACCAGTTGCTGCTAGTGGAGGCGGTGGCCCAGAGGCGTCAAATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

539

Amino Acids

59.23

Weight (kDa)

5.13

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 341 - 493 2e-22 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 419
AccI GTMKAC 1 cut(s) 23
AccII CGCG 1 cut(s) 274
AciI CCGC 6 cut(s) 105, 272, 334, 1263, 1384, 1590
AclWI GGATC 6 cut(s) 104, 262, 308, 564, 770, 1350
AcoI YGGCCR 1 cut(s) 635
AcsI RAATTY 3 cut(s) 168, 875, 1609
AcuI CTGAAG 1 cut(s) 1454
AcvI CACGTG 2 cut(s) 83, 1444
AcyI GRCGYC 1 cut(s) 1604
AfaI GTAC 3 cut(s) 215, 719, 1234
AfiI CCNNNNNNNGG 5 cut(s) 119, 727, 909, 913, 959
AgsI TTSAA 3 cut(s) 577, 740, 832
AhlI ACTAGT 1 cut(s) 1229
AjnI CCWGG 5 cut(s) 236, 250, 316, 766, 820
AloI GAACNNNNNNTCC 2 cut(s) 1168, 1200
AluBI AGCT 6 cut(s) 194, 633, 688, 709, 990, 1556
AluI AGCT 6 cut(s) 194, 633, 688, 709, 990, 1556
Alw26I GTCTC 2 cut(s) 35, 512
AlwI GGATC 6 cut(s) 104, 262, 308, 564, 770, 1350
AlwNI CAGNNNCTG 3 cut(s) 1022, 1559, 1577
AoxI GGCC 5 cut(s) 128, 635, 790, 900, 1594
ApeKI GCWGC 5 cut(s) 331, 845, 1022, 1421, 1577
ApoI RAATTY 3 cut(s) 168, 875, 1609
Asp700I GAANNNNTTC 1 cut(s) 1038
AspLEI GCGC 3 cut(s) 276, 1016, 1376
AspS9I GGNCC 6 cut(s) 128, 791, 890, 901, 941, 1595
AsuHPI GGTGA 4 cut(s) 134, 253, 974, 980
AvaII GGWCC 2 cut(s) 890, 941
BalI TGGCCA 1 cut(s) 637
BanI GGYRCC 1 cut(s) 419
BbrPI CACGTG 2 cut(s) 83, 1444
BbsI GAAGAC 1 cut(s) 1314
BbvI GCAGC 5 cut(s) 318, 832, 1009, 1408, 1564
BccI CCATC 5 cut(s) 725, 782, 787, 1140, 1458
BcgI CGANNNNNNTGC 2 cut(s) 1325, 1359
BciT130I CCWGG 5 cut(s) 238, 252, 318, 768, 822
BclI TGATCA 1 cut(s) 568
BcoDI GTCTC 2 cut(s) 35, 512
BcuI ACTAGT 1 cut(s) 1229
BfaI CTAG 4 cut(s) 116, 191, 1230, 1581
BfmI CTRYAG 1 cut(s) 1551
BfoI RGCGCY 1 cut(s) 1377
BisI GCNGC 5 cut(s) 332, 846, 1023, 1422, 1578
BlsI GCNGC 5 cut(s) 333, 847, 1024, 1423, 1579
BmcAI AGTACT 1 cut(s) 1234
Bme1390I CCNGG 5 cut(s) 238, 252, 318, 768, 822
Bme18I GGWCC 2 cut(s) 890, 941
BmgT120I GGNCC 6 cut(s) 128, 791, 890, 901, 941, 1595
BmiI GGNNCC 1 cut(s) 421
BmrFI CCNGG 5 cut(s) 238, 252, 318, 768, 822
BmsI GCATC 6 cut(s) 49, 285, 701, 832, 1443, 1507
BpiI GAAGAC 1 cut(s) 1314
BpmI CTGGAG 1 cut(s) 843
BsaAI YACGTR 2 cut(s) 83, 1444
BsaHI GRCGYC 1 cut(s) 1604
BsaI GGTCTC 1 cut(s) 35
BsaJI CCNNGG 3 cut(s) 17, 237, 1297
BsaXI ACNNNNNCTCC 2 cut(s) 1168, 1198
Bsc4I CCNNNNNNNGG 5 cut(s) 119, 727, 909, 913, 959
Bse1I ACTGG 1 cut(s) 1571
Bse3DI GCAATG 1 cut(s) 695
BseBI CCWGG 5 cut(s) 238, 252, 318, 768, 822
BseDI CCNNGG 3 cut(s) 17, 237, 1297
BseGI GGATG 6 cut(s) 276, 673, 736, 779, 1057, 1293
BseLI CCNNNNNNNGG 5 cut(s) 119, 727, 909, 913, 959
BseMI GCAATG 1 cut(s) 695
BseMII CTCAG 1 cut(s) 629
BseNI ACTGG 1 cut(s) 1571
BseRI GAGGAG 2 cut(s) 896, 1062
BseXI GCAGC 5 cut(s) 318, 832, 1009, 1408, 1564
BseYI CCCAGC 1 cut(s) 347
Bsh1236I CGCG 1 cut(s) 274
BshFI GGCC 5 cut(s) 130, 637, 792, 902, 1596
BshNI GGYRCC 1 cut(s) 419
BslFI GGGAC 3 cut(s) 364, 1095, 1366
BslI CCNNNNNNNGG 5 cut(s) 119, 727, 909, 913, 959
BsmAI GTCTC 2 cut(s) 35, 512
BsmFI GGGAC 3 cut(s) 364, 1095, 1366
BsmI GAATGC 1 cut(s) 1097
BsnI GGCC 5 cut(s) 130, 637, 792, 902, 1596
Bso31I GGTCTC 1 cut(s) 35
Bsp143I GATC 9 cut(s) 109, 151, 254, 290, 313, 556, 568, 775, 1342
Bsp19I CCATGG 2 cut(s) 17, 1297
BspACI CCGC 6 cut(s) 105, 272, 334, 1263, 1384, 1590
BspANI GGCC 5 cut(s) 130, 637, 792, 902, 1596
BspCNI CTCAG 1 cut(s) 628
BspFNI CGCG 1 cut(s) 274
BspHI TCATGA 1 cut(s) 1101
BspLI GGNNCC 1 cut(s) 421
BspPI GGATC 6 cut(s) 104, 262, 308, 564, 770, 1350
BspT107I GGYRCC 1 cut(s) 419
BspTNI GGTCTC 1 cut(s) 35
BsrDI GCAATG 1 cut(s) 695
BsrI ACTGG 1 cut(s) 1571
BssECI CCNNGG 3 cut(s) 17, 237, 1297
BssMI GATC 9 cut(s) 109, 151, 254, 290, 313, 556, 568, 775, 1342
BssNI GRCGYC 1 cut(s) 1604
BssT1I CCWWGG 2 cut(s) 17, 1297
Bst2UI CCWGG 5 cut(s) 238, 252, 318, 768, 822
Bst4CI ACNGT 2 cut(s) 136, 523
Bst6I CTCTTC 2 cut(s) 488, 801
BstACI GRCGYC 1 cut(s) 1604
BstAPI GCANNNNNTGC 1 cut(s) 1022
BstBAI YACGTR 2 cut(s) 83, 1444
BstC8I GCNNGC 6 cut(s) 105, 635, 690, 883, 915, 1012
BstDEI CTNAG 3 cut(s) 615, 1537, 1614
BstDSI CCRYGG 2 cut(s) 17, 1297
BstF5I GGATG 6 cut(s) 276, 673, 736, 779, 1057, 1293
BstFNI CGCG 1 cut(s) 274
BstH2I RGCGCY 1 cut(s) 1377
BstHHI GCGC 3 cut(s) 276, 1016, 1376
BstKTI GATC 9 cut(s) 112, 154, 257, 293, 316, 559, 571, 778, 1345
BstMAI GTCTC 2 cut(s) 35, 512
BstMBI GATC 9 cut(s) 109, 151, 254, 290, 313, 556, 568, 775, 1342
BstMWI GCNNNNNNNGC 3 cut(s) 706, 1022, 1288
BstNI CCWGG 5 cut(s) 238, 252, 318, 768, 822
BstNSI RCATGY 1 cut(s) 1127
BstSCI CCNGG 5 cut(s) 236, 250, 316, 766, 820
BstSFI CTRYAG 1 cut(s) 1551
BstUI CGCG 1 cut(s) 274
BstV1I GCAGC 5 cut(s) 318, 832, 1009, 1408, 1564
BstV2I GAAGAC 1 cut(s) 1314
BstX2I RGATCY 1 cut(s) 556
BstYI RGATCY 1 cut(s) 556
BsuRI GGCC 5 cut(s) 130, 637, 792, 902, 1596
BtgI CCRYGG 2 cut(s) 17, 1297
BtsCI GGATG 6 cut(s) 276, 673, 736, 779, 1057, 1293
Cac8I GCNNGC 6 cut(s) 105, 635, 690, 883, 915, 1012
CaiI CAGNNNCTG 3 cut(s) 1022, 1559, 1577
CciI TCATGA 1 cut(s) 1101
CfoI GCGC 3 cut(s) 276, 1016, 1376
Cfr13I GGNCC 6 cut(s) 128, 791, 890, 901, 941, 1595
CseI GACGC 1 cut(s) 1593
Csp6I GTAC 3 cut(s) 214, 718, 1233
CviAII CATG 9 cut(s) 18, 1102, 1124, 1258, 1298, 1333, 1370, 1412, 1438
CviQI GTAC 3 cut(s) 214, 718, 1233
DdeI CTNAG 3 cut(s) 615, 1537, 1614
DpnI GATC 9 cut(s) 111, 153, 256, 292, 315, 558, 570, 777, 1344
DpnII GATC 9 cut(s) 109, 151, 254, 290, 313, 556, 568, 775, 1342
EaeI YGGCCR 1 cut(s) 635
Eam1104I CTCTTC 2 cut(s) 488, 801
EarI CTCTTC 2 cut(s) 488, 801
Eco130I CCWWGG 2 cut(s) 17, 1297
Eco31I GGTCTC 1 cut(s) 35
Eco47I GGWCC 2 cut(s) 890, 941
Eco57I CTGAAG 1 cut(s) 1454
Eco72I CACGTG 2 cut(s) 83, 1444
EcoRII CCWGG 5 cut(s) 236, 250, 316, 766, 820
EcoT14I CCWWGG 2 cut(s) 17, 1297
ErhI CCWWGG 2 cut(s) 17, 1297
FaeI CATG 9 cut(s) 21, 1105, 1127, 1261, 1301, 1336, 1373, 1415, 1441
FalI AAGNNNNNCTT 2 cut(s) 1271, 1303
FaqI GGGAC 3 cut(s) 364, 1095, 1366
FatI CATG 9 cut(s) 17, 1101, 1123, 1257, 1297, 1332, 1369, 1411, 1437
FauI CCCGC 2 cut(s) 112, 1391
FauNDI CATATG 3 cut(s) 28, 355, 1090
FbaI TGATCA 1 cut(s) 568
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 5 cut(s) 332, 846, 1023, 1422, 1578
FokI GGATG 6 cut(s) 263, 660, 743, 766, 1044, 1280
Fsp4HI GCNGC 5 cut(s) 332, 846, 1023, 1422, 1578
FspBI CTAG 4 cut(s) 116, 191, 1230, 1581
GlaI GCGC 3 cut(s) 275, 1015, 1375
GluI GCNGC 5 cut(s) 332, 846, 1023, 1422, 1578
GsaI CCCAGC 1 cut(s) 351
GsuI CTGGAG 1 cut(s) 843
HaeII RGCGCY 1 cut(s) 1377
HaeIII GGCC 5 cut(s) 130, 637, 792, 902, 1596
HgaI GACGC 1 cut(s) 1593
HhaI GCGC 3 cut(s) 276, 1016, 1376
Hin1I GRCGYC 1 cut(s) 1604
Hin1II CATG 9 cut(s) 21, 1105, 1127, 1261, 1301, 1336, 1373, 1415, 1441
Hin6I GCGC 3 cut(s) 274, 1014, 1374
HinP1I GCGC 3 cut(s) 274, 1014, 1374
HinfI GANTC 7 cut(s) 185, 206, 596, 1249, 1393, 1408, 1511
HphI GGTGA 4 cut(s) 134, 253, 974, 980
Hpy166II GTNNAC 2 cut(s) 24, 202
Hpy188III TCNNGA 5 cut(s) 783, 939, 1061, 1102, 1320
Hpy8I GTNNAC 2 cut(s) 24, 202
Hpy99I CGWCG 1 cut(s) 1047
HpyAV CCTTC 9 cut(s) 47, 113, 119, 370, 383, 545, 721, 770, 915
HpyCH4III ACNGT 2 cut(s) 136, 523
HpyCH4IV ACGT 3 cut(s) 82, 1156, 1443
HpyF10VI GCNNNNNNNGC 3 cut(s) 706, 1022, 1288
HpyF3I CTNAG 3 cut(s) 615, 1537, 1614
HpySE526I ACGT 3 cut(s) 82, 1156, 1443
Hsp92I GRCGYC 1 cut(s) 1604
Hsp92II CATG 9 cut(s) 21, 1105, 1127, 1261, 1301, 1336, 1373, 1415, 1441
HspAI GCGC 3 cut(s) 274, 1014, 1374
Ksp22I TGATCA 1 cut(s) 568
Kzo9I GATC 9 cut(s) 109, 151, 254, 290, 313, 556, 568, 775, 1342
LmnI GCTCC 2 cut(s) 997, 1132
Lsp1109I GCAGC 5 cut(s) 318, 832, 1009, 1408, 1564
LweI GCATC 6 cut(s) 49, 285, 701, 832, 1443, 1507
MaeI CTAG 4 cut(s) 116, 191, 1230, 1581
MaeII ACGT 3 cut(s) 82, 1156, 1443
MaeIII GTNAC 1 cut(s) 497
MalI GATC 9 cut(s) 111, 153, 256, 292, 315, 558, 570, 777, 1344
MboI GATC 9 cut(s) 109, 151, 254, 290, 313, 556, 568, 775, 1342
MboII GAAGA 7 cut(s) 146, 311, 505, 788, 1316, 1319, 1388
MfeI CAATTG 1 cut(s) 1001
MflI RGATCY 1 cut(s) 556
MlsI TGGCCA 1 cut(s) 637
MluNI TGGCCA 1 cut(s) 637
MlyI GAGTC 4 cut(s) 215, 605, 1258, 1417
MmeI TCCRAC 2 cut(s) 917, 1320
Mox20I TGGCCA 1 cut(s) 637
MroXI GAANNNNTTC 1 cut(s) 1038
MscI TGGCCA 1 cut(s) 637
MseI TTAA 4 cut(s) 533, 1275, 1328, 1353
MslI CAYNNNNRTG 3 cut(s) 1089, 1416, 1442
Msp20I TGGCCA 1 cut(s) 637
MspA1I CMGCKG 2 cut(s) 107, 688
MspR9I CCNGG 5 cut(s) 238, 252, 318, 768, 822
MunI CAATTG 1 cut(s) 1001
Mva1269I GAATGC 1 cut(s) 1097
MvaI CCWGG 5 cut(s) 238, 252, 318, 768, 822
MvnI CGCG 1 cut(s) 274
MwoI GCNNNNNNNGC 3 cut(s) 706, 1022, 1288
NcoI CCATGG 2 cut(s) 17, 1297
NdeI CATATG 3 cut(s) 28, 355, 1090
NdeII GATC 9 cut(s) 109, 151, 254, 290, 313, 556, 568, 775, 1342
NlaIII CATG 9 cut(s) 21, 1105, 1127, 1261, 1301, 1336, 1373, 1415, 1441
NlaIV GGNNCC 1 cut(s) 421
NmuCI GTSAC 1 cut(s) 497
NspI RCATGY 1 cut(s) 1127
PagI TCATGA 1 cut(s) 1101
PctI GAATGC 1 cut(s) 1097
PdmI GAANNNNTTC 1 cut(s) 1038
PfeI GAWTC 3 cut(s) 185, 1393, 1511
PfoI TCCNGGA 2 cut(s) 250, 820
PkrI GCNGC 5 cut(s) 333, 847, 1024, 1423, 1579
PleI GAGTC 4 cut(s) 214, 604, 1257, 1416
PmaCI CACGTG 2 cut(s) 83, 1444
PmlI CACGTG 2 cut(s) 83, 1444
PpsI GAGTC 4 cut(s) 214, 604, 1257, 1416
Ppu21I YACGTR 2 cut(s) 83, 1444
Psp6I CCWGG 5 cut(s) 236, 250, 316, 766, 820
PspCI CACGTG 2 cut(s) 83, 1444
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 5 cut(s) 236, 250, 316, 766, 820
PspN4I GGNNCC 1 cut(s) 421
PspPI GGNCC 6 cut(s) 128, 791, 890, 901, 941, 1595
PstNI CAGNNNCTG 3 cut(s) 1022, 1559, 1577
PsuI RGATCY 1 cut(s) 556
PvuII CAGCTG 1 cut(s) 688
RsaI GTAC 3 cut(s) 215, 719, 1234
RsaNI GTAC 3 cut(s) 214, 718, 1233
RseI CAYNNNNRTG 3 cut(s) 1089, 1416, 1442
SaqAI TTAA 4 cut(s) 533, 1275, 1328, 1353
SatI GCNGC 5 cut(s) 332, 846, 1023, 1422, 1578
Sau3AI GATC 9 cut(s) 109, 151, 254, 290, 313, 556, 568, 775, 1342
Sau96I GGNCC 6 cut(s) 128, 791, 890, 901, 941, 1595
ScaI AGTACT 1 cut(s) 1234
SchI GAGTC 4 cut(s) 215, 605, 1258, 1417
ScrFI CCNGG 5 cut(s) 238, 252, 318, 768, 822
SfaNI GCATC 6 cut(s) 49, 285, 701, 832, 1443, 1507
SfcI CTRYAG 1 cut(s) 1551
SinI GGWCC 2 cut(s) 890, 941
SmiMI CAYNNNNRTG 3 cut(s) 1089, 1416, 1442
SpeI ACTAGT 1 cut(s) 1229
SsiI CCGC 6 cut(s) 105, 272, 334, 1263, 1384, 1590
SspI AATATT 2 cut(s) 582, 1478
SspMI CTAG 4 cut(s) 116, 191, 1230, 1581
StyD4I CCNGG 5 cut(s) 236, 250, 316, 766, 820
StyI CCWWGG 2 cut(s) 17, 1297
TaaI ACNGT 2 cut(s) 136, 523
TaiI ACGT 3 cut(s) 85, 1159, 1446
TaqI TCGA 3 cut(s) 450, 919, 1042
TatI WGTACW 1 cut(s) 1232
TfiI GAWTC 3 cut(s) 185, 1393, 1511
Tru1I TTAA 4 cut(s) 533, 1275, 1328, 1353
Tru9I TTAA 4 cut(s) 533, 1275, 1328, 1353
TseFI GTSAC 1 cut(s) 497
TseI GCWGC 5 cut(s) 331, 845, 1022, 1421, 1577
Tsp45I GTSAC 1 cut(s) 497
TspDTI ATGAA 6 cut(s) 66, 558, 888, 1077, 1090, 1246
TspGWI ACGGA 2 cut(s) 238, 789
VpaK11BI GGWCC 2 cut(s) 890, 941
XapI RAATTY 3 cut(s) 168, 875, 1609
XceI RCATGY 1 cut(s) 1127
XcmI CCANNNNNNNNNTGG 1 cut(s) 728
XmiI GTMKAC 1 cut(s) 23
XmnI GAANNNNTTC 1 cut(s) 1038
XspI CTAG 4 cut(s) 116, 191, 1230, 1581
ZrmI AGTACT 1 cut(s) 1234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.