FvH4_6g13000

protein At2g39795, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008277
Physical Location & Seq
Reverse (-)
7881401 .. 7883216
1816 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g13000.t1

Sequence Viewer

Length: 765 bp
ATGGCTTTCTTCTCGTCGATGCTTCGCAACCCATCCCGCTCCTTATTCTCCCTCGCCCTCCGCACCGTCGGATCTGCAAGAACCTTCCACAGCCGCACCTCCACCGTCCCCAACTTCGGCACTGAGAATGTCACCCGACAATGCTCTGTTCCATTTCTGCGTCTCTCCACCGCCACCGCCGTGAAACCCGCCGCCGACGACACTCTGGTCGGAGTTCTCGAGTCTGAGATCAGGGTCGCTGAAGAAGACATACAAGATCATCAGGTAGGTGAGGTTGCAGATGAGTTTCCTTTTGAAATAGTAGACAATGCTGGAGAGCAAACCATAATGCTCCGAAGAGAATTTGATGGAGAAATCATTAAAGTGGCGGTTGACGTTCCTAATGTTTCTCCCACCGAGGACAATGATGATGAGGAGGAGGAGGAGGAGGAGGATGAGGACACTGGGATTCCGATGGTTGTGAGTGTTTCGAAAGGAAATGGGAAGGAGCTGGAGTTTGGGCTCACTGCCTTTACTGATGAGATTTGCATTGATAGCTTGTTGATCAGGCAGCCAGAGGATTCAGAAGGTTCGAAGCGGATTGCATATGAAGGGCCTAATTTTAGTGACTTGGATAAGAGCTTTCAGAAGGCCTTCCAGAAATATCTGGAGATTCGAGGGATTAAGTCGAGCATAACCAAGTTCCTGCAGGAGTATATGAAGAACAAAGACTACAGAGAGTACTTGCTGTGGTTAAAGAACCTAAAGGGTTTTATTGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
MAM33

Protein Analysis

255

Amino Acids

28.7

Weight (kDa)

4.51

Isoelectric Point (pI)

51.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAM33 PF02330 73 - 252 6.8e-45 Mitochondrial glycoprotein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 206
AccBSI CCGCTC 1 cut(s) 39
AccI GTMKAC 1 cut(s) 303
AciI CCGC 9 cut(s) 37, 61, 94, 171, 177, 189, 192, 368, 577
AclWI GGATC 1 cut(s) 79
AcsI RAATTY 1 cut(s) 341
AcuI CTGAAG 1 cut(s) 261
AfaI GTAC 1 cut(s) 722
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 2 cut(s) 296, 758
AleI CACNNNNGTG 1 cut(s) 179
AluBI AGCT 3 cut(s) 490, 537, 621
AluI AGCT 3 cut(s) 490, 537, 621
Alw26I GTCTC 1 cut(s) 167
AlwI GGATC 1 cut(s) 79
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 2 cut(s) 593, 630
ApeKI GCWGC 1 cut(s) 550
ApoI RAATTY 1 cut(s) 341
Asp700I GAANNNNTTC 1 cut(s) 632
AspS9I GGNCC 1 cut(s) 593
AsuHPI GGTGA 2 cut(s) 124, 281
AsuII TTCGAA 2 cut(s) 470, 572
AvaI CYCGRG 1 cut(s) 218
BanII GRGCYC 1 cut(s) 504
BarI GAAGNNNNNNTAC 2 cut(s) 234, 266
BbsI GAAGAC 1 cut(s) 252
BbvI GCAGC 1 cut(s) 562
BccI CCATC 3 cut(s) 40, 341, 448
BceAI ACGGC 1 cut(s) 164
BclI TGATCA 1 cut(s) 543
BcoDI GTCTC 1 cut(s) 167
BfmI CTRYAG 2 cut(s) 686, 712
BisI GCNGC 3 cut(s) 94, 192, 551
BlsI GCNGC 3 cut(s) 95, 193, 552
BmcAI AGTACT 1 cut(s) 722
BmeT110I CYCGRG 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 593
BmrI ACTGGG 1 cut(s) 453
BmsI GCATC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 453
BpiI GAAGAC 1 cut(s) 252
BpmI CTGGAG 3 cut(s) 333, 512, 668
Bpu14I TTCGAA 2 cut(s) 470, 572
BsaJI CCNNGG 1 cut(s) 396
BsaXI ACNNNNNCTCC 2 cut(s) 479, 509
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 448
BseDI CCNNGG 1 cut(s) 396
BseGI GGATG 2 cut(s) 32, 439
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMII CTCAG 2 cut(s) 114, 216
BseNI ACTGG 1 cut(s) 448
BseRI GAGGAG 6 cut(s) 428, 431, 434, 437, 440, 443
BseXI GCAGC 1 cut(s) 562
BshFI GGCC 2 cut(s) 595, 632
BsiHKCI CYCGRG 1 cut(s) 218
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 167
BsmBI CGTCTC 1 cut(s) 167
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 2 cut(s) 595, 632
BsoBI CYCGRG 1 cut(s) 218
Bsp119I TTCGAA 2 cut(s) 470, 572
Bsp1286I GDGCHC 1 cut(s) 504
Bsp143I GATC 4 cut(s) 71, 228, 256, 543
BspACI CCGC 9 cut(s) 37, 61, 94, 171, 177, 189, 192, 368, 577
BspANI GGCC 2 cut(s) 595, 632
BspCNI CTCAG 2 cut(s) 115, 217
BspMAI CTGCAG 1 cut(s) 690
BspPI GGATC 1 cut(s) 79
BspT104I TTCGAA 2 cut(s) 470, 572
BsrBI CCGCTC 1 cut(s) 39
BsrI ACTGG 1 cut(s) 448
BssECI CCNNGG 1 cut(s) 396
BssMI GATC 4 cut(s) 71, 228, 256, 543
Bst4CI ACNGT 2 cut(s) 67, 106
Bst6I CTCTTC 2 cut(s) 331, 753
BstBI TTCGAA 2 cut(s) 470, 572
BstDEI CTNAG 2 cut(s) 123, 225
BstF5I GGATG 2 cut(s) 32, 439
BstKTI GATC 4 cut(s) 74, 231, 259, 546
BstMAI GTCTC 1 cut(s) 167
BstMBI GATC 4 cut(s) 71, 228, 256, 543
BstMWI GCNNNNNNNGC 1 cut(s) 534
BstSFI CTRYAG 2 cut(s) 686, 712
BstV1I GCAGC 1 cut(s) 562
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 1 cut(s) 71
BstYI RGATCY 1 cut(s) 71
BsuRI GGCC 2 cut(s) 595, 632
BtsCI GGATG 2 cut(s) 32, 439
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 3 cut(s) 120, 441, 504
Cfr13I GGNCC 1 cut(s) 593
CseI GACGC 1 cut(s) 149
Csp6I GTAC 1 cut(s) 721
CviJI RGCY 9 cut(s) 5, 93, 490, 502, 537, 553, 595, 621, 632
CviKI_1 RGCY 9 cut(s) 5, 93, 490, 502, 537, 553, 595, 621, 632
CviQI GTAC 1 cut(s) 721
DdeI CTNAG 2 cut(s) 123, 225
DpnI GATC 4 cut(s) 73, 230, 258, 545
DpnII GATC 4 cut(s) 71, 228, 256, 543
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
Eam1104I CTCTTC 2 cut(s) 331, 753
EarI CTCTTC 2 cut(s) 331, 753
Eco147I AGGCCT 1 cut(s) 632
Eco24I GRGCYC 1 cut(s) 504
Eco57I CTGAAG 1 cut(s) 261
Eco88I CYCGRG 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 593
EcoT38I GRGCYC 1 cut(s) 504
Esp3I CGTCTC 1 cut(s) 167
FaiI YATR 7 cut(s) 251, 326, 586, 588, 674, 696, 698
FaqI GGGAC 1 cut(s) 92
FauI CCCGC 2 cut(s) 44, 196
FauNDI CATATG 1 cut(s) 586
FbaI TGATCA 1 cut(s) 543
FblI GTMKAC 1 cut(s) 303
Fnu4HI GCNGC 3 cut(s) 94, 192, 551
FokI GGATG 2 cut(s) 19, 446
FriOI GRGCYC 1 cut(s) 504
Fsp4HI GCNGC 3 cut(s) 94, 192, 551
GluI GCNGC 3 cut(s) 94, 192, 551
GsuI CTGGAG 3 cut(s) 333, 512, 668
HaeIII GGCC 2 cut(s) 595, 632
HgaI GACGC 1 cut(s) 149
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HinfI GANTC 4 cut(s) 221, 448, 560, 652
HphI GGTGA 2 cut(s) 124, 281
Hpy166II GTNNAC 2 cut(s) 304, 373
Hpy188I TCNGA 7 cut(s) 71, 212, 226, 335, 453, 565, 627
Hpy188III TCNNGA 3 cut(s) 218, 637, 647
Hpy8I GTNNAC 2 cut(s) 304, 373
Hpy99I CGWCG 3 cut(s) 19, 71, 200
HpyAV CCTTC 6 cut(s) 94, 478, 560, 584, 622, 643
HpyCH4III ACNGT 2 cut(s) 67, 106
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 5 cut(s) 77, 278, 528, 584, 688
HpyF10VI GCNNNNNNNGC 1 cut(s) 534
HpyF3I CTNAG 2 cut(s) 123, 225
HpySE526I ACGT 1 cut(s) 375
Ksp22I TGATCA 1 cut(s) 543
Kzo9I GATC 4 cut(s) 71, 228, 256, 543
LmnI GCTCC 3 cut(s) 44, 336, 487
Lsp1109I GCAGC 1 cut(s) 562
LweI GCATC 1 cut(s) 9
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 2 cut(s) 130, 605
MalI GATC 4 cut(s) 73, 230, 258, 545
MbiI CCGCTC 1 cut(s) 39
MboI GATC 4 cut(s) 71, 228, 256, 543
MboII GAAGA 4 cut(s) 254, 257, 348, 712
MflI RGATCY 1 cut(s) 71
MhlI GDGCHC 1 cut(s) 504
MluCI AATT 2 cut(s) 341, 598
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 2 cut(s) 49, 190
MroXI GAANNNNTTC 1 cut(s) 632
MseI TTAA 3 cut(s) 360, 663, 734
MslI CAYNNNNRTG 2 cut(s) 179, 362
MwoI GCNNNNNNNGC 1 cut(s) 534
NdeI CATATG 1 cut(s) 586
NdeII GATC 4 cut(s) 71, 228, 256, 543
NmuCI GTSAC 2 cut(s) 130, 605
NspV TTCGAA 2 cut(s) 470, 572
OliI CACNNNNGTG 1 cut(s) 179
PaeR7I CTCGAG 1 cut(s) 218
PceI AGGCCT 1 cut(s) 632
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 632
PfeI GAWTC 3 cut(s) 448, 560, 652
PkrI GCNGC 3 cut(s) 95, 193, 552
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
PspPI GGNCC 1 cut(s) 593
PsrI GAACNNNNNNTAC 2 cut(s) 695, 727
PstI CTGCAG 1 cut(s) 690
PsuI RGATCY 1 cut(s) 71
RsaI GTAC 1 cut(s) 722
RsaNI GTAC 1 cut(s) 721
RseI CAYNNNNRTG 2 cut(s) 179, 362
SaqAI TTAA 3 cut(s) 360, 663, 734
SatI GCNGC 3 cut(s) 94, 192, 551
Sau3AI GATC 4 cut(s) 71, 228, 256, 543
Sau96I GGNCC 1 cut(s) 593
SbfI CCTGCAGG 1 cut(s) 690
ScaI AGTACT 1 cut(s) 722
SchI GAGTC 1 cut(s) 230
SdaI CCTGCAGG 1 cut(s) 690
SduI GDGCHC 1 cut(s) 504
SfaNI GCATC 1 cut(s) 9
SfcI CTRYAG 2 cut(s) 686, 712
Sfr274I CTCGAG 1 cut(s) 218
SfuI TTCGAA 2 cut(s) 470, 572
SlaI CTCGAG 1 cut(s) 218
SmiMI CAYNNNNRTG 2 cut(s) 179, 362
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse8387I CCTGCAGG 1 cut(s) 690
Sse9I AATT 2 cut(s) 341, 598
SseBI AGGCCT 1 cut(s) 632
SsiI CCGC 9 cut(s) 37, 61, 94, 171, 177, 189, 192, 368, 577
StuI AGGCCT 1 cut(s) 632
TaaI ACNGT 2 cut(s) 67, 106
TaiI ACGT 1 cut(s) 378
TaqI TCGA 6 cut(s) 17, 219, 470, 572, 655, 668
TasI AATT 2 cut(s) 341, 598
TatI WGTACW 1 cut(s) 720
TauI GCSGC 2 cut(s) 96, 194
TfiI GAWTC 3 cut(s) 448, 560, 652
Tru1I TTAA 3 cut(s) 360, 663, 734
Tru9I TTAA 3 cut(s) 360, 663, 734
TscAI CASTG 3 cut(s) 127, 448, 511
TseFI GTSAC 2 cut(s) 130, 605
TseI GCWGC 1 cut(s) 550
Tsp45I GTSAC 2 cut(s) 130, 605
TspDTI ATGAA 2 cut(s) 603, 713
TspRI CASTG 3 cut(s) 127, 448, 511
XapI RAATTY 1 cut(s) 341
XhoI CTCGAG 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 303
XmnI GAANNNNTTC 1 cut(s) 632
ZrmI AGTACT 1 cut(s) 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.