MD00G1141400.v1.1

protein At2g39795, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0008277
Physical Location & Seq
Reverse (-)
30862058 .. 30864235
2178 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1141400.v1.1.491

Sequence Viewer

Length: 753 bp
ATGTCTCTCTTCTCGATGCTCCGCAGGCCGTCCTACTCCGCCCTATCCCTCGCCGTCCTCTCCGTCCGCGATGTCGGGTCAGCACCCATCTTTCACCGCGTGATCTCCACCGTCCCTACTTTCAGCCCTCAGCTCATCCGGAGAAGCTTTGTTCCAATTCATCCATTCTCCACTGCCACTGCCACTGCCACAGCCGTGGAGCCGATCAGCGAACGAAGTCTGGTCGAAGTCCTTGAGTCCGAGATCAAGTGTGCCCAAGACGTGAAACCCAAAGAAGCCGAGGTCCCAGAGAAGTTTCCTTTTCAAATAGTAGACAATCCTGGAGAACAAACCATTTTGCTCAAAAGAGAATTTGAAGGCGAAACCATCCAAGTGTGTGTCGATATTCCTAGTGTTTCCACAGGTGAAGACGATTGGAAGTGTGAAGACCCCGGGATTCCGATGGTTGTGAGCATTTCCAAACCAAATGGACTGCAATTGGAGCTTGGTGTTTCTGCTTTTGCAACTGAGATTTTGATCGATTGCTTGTCGGTCAAGCAGCCTGAGCGTTGTTCCAAAGACCTTGATTATGCTTATGATGGACCTGAATTTCATGACTTGGATGAGGAGTTGCAGAATGCTTTCTACAAGTATCTCGAGGGTCGAGGGATCGAACGCACCATGTCTGACTTCTTGTATGATTACATGGAGAACAAAGACAAGAAAGAGTACCTGATTTGGTTGAAGAAAGCCAAGGGTTTAGTTCAGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
MAM33

Protein Analysis

251

Amino Acids

28.2

Weight (kDa)

4.84

Isoelectric Point (pI)

53.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAM33 PF02330 78 - 247 2.2e-39 Mitochondrial glycoprotein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 312
AccII CGCG 2 cut(s) 69, 99
AccIII TCCGGA 1 cut(s) 138
AciI CCGC 4 cut(s) 22, 39, 67, 97
AclWI GGATC 1 cut(s) 656
AcsI RAATTY 2 cut(s) 350, 587
AdeI CACNNNGTG 1 cut(s) 100
AfaI GTAC 1 cut(s) 710
AgsI TTSAA 3 cut(s) 305, 356, 724
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 1 cut(s) 319
AleI CACNNNNGTG 1 cut(s) 194
AluBI AGCT 3 cut(s) 133, 147, 484
AluI AGCT 3 cut(s) 133, 147, 484
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 656
Ama87I CYCGRG 2 cut(s) 431, 635
Aor13HI TCCGGA 1 cut(s) 138
AoxI GGCC 1 cut(s) 26
ApeKI GCWGC 1 cut(s) 538
ApoI RAATTY 2 cut(s) 350, 587
Asp700I GAANNNNTTC 1 cut(s) 620
AspS9I GGNCC 2 cut(s) 283, 581
AsuC2I CCSGG 2 cut(s) 432, 433
AsuHPI GGTGA 2 cut(s) 86, 416
AvaI CYCGRG 2 cut(s) 431, 635
AvaII GGWCC 2 cut(s) 283, 581
BaeGI GKGCMC 1 cut(s) 256
BbsI GAAGAC 2 cut(s) 414, 432
BbvCI CCTCAGC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 550
BccI CCATC 4 cut(s) 95, 374, 436, 572
BceAI ACGGC 3 cut(s) 13, 38, 179
BciT130I CCWGG 1 cut(s) 321
BcnI CCSGG 2 cut(s) 432, 433
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 1 cut(s) 390
BisI GCNGC 1 cut(s) 539
BlsI GCNGC 1 cut(s) 540
Bme1390I CCNGG 3 cut(s) 321, 432, 433
Bme18I GGWCC 2 cut(s) 283, 581
BmeT110I CYCGRG 2 cut(s) 431, 635
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 2 cut(s) 283, 581
BmiI GGNNCC 2 cut(s) 201, 285
BmrFI CCNGG 3 cut(s) 321, 432, 433
BmsI GCATC 1 cut(s) 6
BpiI GAAGAC 2 cut(s) 414, 432
BpmI CTGGAG 1 cut(s) 342
Bpu10I CCTNAGC 2 cut(s) 129, 543
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 2 cut(s) 432, 433
Bsa29I ATCGAT 1 cut(s) 519
BsaBI GATNNNNATC 1 cut(s) 515
BsaJI CCNNGG 5 cut(s) 195, 279, 430, 431, 732
BsaWI WCCGGW 1 cut(s) 138
Bse8I GATNNNNATC 1 cut(s) 515
BseAI TCCGGA 1 cut(s) 138
BseBI CCWGG 1 cut(s) 321
BseCI ATCGAT 1 cut(s) 519
BseDI CCNNGG 5 cut(s) 195, 279, 430, 431, 732
BseGI GGATG 4 cut(s) 135, 160, 366, 607
BseJI GATNNNNATC 1 cut(s) 515
BseMII CTCAG 3 cut(s) 143, 498, 534
BseRI GAGGAG 1 cut(s) 620
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 1 cut(s) 550
Bsh1236I CGCG 2 cut(s) 69, 99
BshFI GGCC 1 cut(s) 28
BshVI ATCGAT 1 cut(s) 519
BsiHKCI CYCGRG 2 cut(s) 431, 635
BsiSI CCGG 2 cut(s) 139, 432
BslFI GGGAC 2 cut(s) 98, 269
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 98, 269
BsmI GAATGC 1 cut(s) 622
BsnI GGCC 1 cut(s) 28
BsoBI CYCGRG 2 cut(s) 431, 635
Bsp1286I GDGCHC 1 cut(s) 256
Bsp13I TCCGGA 1 cut(s) 138
Bsp143I GATC 5 cut(s) 102, 204, 243, 516, 648
BspACI CCGC 4 cut(s) 22, 39, 67, 97
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 3 cut(s) 142, 499, 535
BspDI ATCGAT 1 cut(s) 519
BspEI TCCGGA 1 cut(s) 138
BspFNI CGCG 2 cut(s) 69, 99
BspHI TCATGA 1 cut(s) 592
BspLI GGNNCC 2 cut(s) 201, 285
BspPI GGATC 1 cut(s) 656
BssECI CCNNGG 5 cut(s) 195, 279, 430, 431, 732
BssMI GATC 5 cut(s) 102, 204, 243, 516, 648
BssT1I CCWWGG 1 cut(s) 732
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 1 cut(s) 112
Bst6I CTCTTC 1 cut(s) 14
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 3 cut(s) 129, 507, 543
BstDSI CCRYGG 1 cut(s) 195
BstF5I GGATG 4 cut(s) 135, 160, 366, 607
BstFNI CGCG 2 cut(s) 69, 99
BstKTI GATC 5 cut(s) 105, 207, 246, 519, 651
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 5 cut(s) 102, 204, 243, 516, 648
BstMWI GCNNNNNNNGC 3 cut(s) 25, 481, 544
BstNI CCWGG 1 cut(s) 321
BstSCI CCNGG 3 cut(s) 319, 430, 431
BstSLI GKGCMC 1 cut(s) 256
BstUI CGCG 2 cut(s) 69, 99
BstV1I GCAGC 1 cut(s) 550
BstV2I GAAGAC 2 cut(s) 414, 432
BstXI CCANNNNNNTGG 1 cut(s) 196
Bsu15I ATCGAT 1 cut(s) 519
BsuRI GGCC 1 cut(s) 28
BsuTUI ATCGAT 1 cut(s) 519
BtgI CCRYGG 1 cut(s) 195
BtgZI GCGATG 1 cut(s) 84
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 4 cut(s) 135, 160, 366, 607
BtsI GCAGTG 3 cut(s) 171, 177, 183
BtsIMutI CAGTG 3 cut(s) 171, 177, 183
Cac8I GCNNGC 1 cut(s) 26
CciI TCATGA 1 cut(s) 592
Cfr13I GGNCC 2 cut(s) 283, 581
Cfr9I CCCGGG 1 cut(s) 431
ClaI ATCGAT 1 cut(s) 519
Csp6I GTAC 1 cut(s) 709
CviAII CATG 3 cut(s) 593, 661, 685
CviQI GTAC 1 cut(s) 709
DdeI CTNAG 3 cut(s) 129, 507, 543
DpnI GATC 5 cut(s) 104, 206, 245, 518, 650
DpnII GATC 5 cut(s) 102, 204, 243, 516, 648
DraIII CACNNNGTG 1 cut(s) 100
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
EciI GGCGGA 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 732
Eco47I GGWCC 2 cut(s) 283, 581
Eco88I CYCGRG 2 cut(s) 431, 635
EcoO109I RGGNCCY 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 319
EcoT14I CCWWGG 1 cut(s) 732
ErhI CCWWGG 1 cut(s) 732
FaeI CATG 3 cut(s) 596, 664, 688
FaiI YATR 6 cut(s) 570, 576, 594, 662, 678, 686
FaqI GGGAC 2 cut(s) 98, 269
FatI CATG 3 cut(s) 592, 660, 684
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 1 cut(s) 539
FokI GGATG 4 cut(s) 122, 147, 353, 614
Fsp4HI GCNGC 1 cut(s) 539
FspBI CTAG 1 cut(s) 390
GluI GCNGC 1 cut(s) 539
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 1 cut(s) 28
HapII CCGG 2 cut(s) 139, 432
Hin1II CATG 3 cut(s) 596, 664, 688
HindIII AAGCTT 1 cut(s) 145
HinfI GANTC 2 cut(s) 236, 436
HpaII CCGG 2 cut(s) 139, 432
HphI GGTGA 2 cut(s) 86, 416
Hpy166II GTNNAC 1 cut(s) 313
Hpy188I TCNGA 3 cut(s) 241, 441, 667
Hpy188III TCNNGA 4 cut(s) 13, 139, 593, 635
Hpy8I GTNNAC 1 cut(s) 313
HpyAV CCTTC 1 cut(s) 350
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 3 cut(s) 475, 503, 613
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 481, 544
HpyF3I CTNAG 3 cut(s) 129, 507, 543
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 3 cut(s) 596, 664, 688
Kpn2I TCCGGA 1 cut(s) 138
Kzo9I GATC 5 cut(s) 102, 204, 243, 516, 648
LmnI GCTCC 3 cut(s) 24, 199, 481
Lsp1109I GCAGC 1 cut(s) 550
LweI GCATC 1 cut(s) 6
MaeI CTAG 1 cut(s) 390
MaeII ACGT 1 cut(s) 261
MalI GATC 5 cut(s) 104, 206, 245, 518, 650
MboI GATC 5 cut(s) 102, 204, 243, 516, 648
MboII GAAGA 3 cut(s) 419, 437, 736
MfeI CAATTG 1 cut(s) 476
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 4 cut(s) 156, 350, 476, 587
MlyI GAGTC 1 cut(s) 245
MnlI CCTC 7 cut(s) 59, 68, 138, 274, 598, 631, 638
MroI TCCGGA 1 cut(s) 138
MroXI GAANNNNTTC 1 cut(s) 620
MslI CAYNNNNRTG 2 cut(s) 194, 371
MspI CCGG 2 cut(s) 139, 432
MspR9I CCNGG 3 cut(s) 321, 432, 433
MunI CAATTG 1 cut(s) 476
Mva1269I GAATGC 1 cut(s) 622
MvaI CCWGG 1 cut(s) 321
MvnI CGCG 2 cut(s) 69, 99
MwoI GCNNNNNNNGC 3 cut(s) 25, 481, 544
NciI CCSGG 2 cut(s) 432, 433
NdeII GATC 5 cut(s) 102, 204, 243, 516, 648
NlaIII CATG 3 cut(s) 596, 664, 688
NlaIV GGNNCC 2 cut(s) 201, 285
NmeAIII GCCGAG 1 cut(s) 304
OliI CACNNNNGTG 1 cut(s) 194
PaeR7I CTCGAG 1 cut(s) 635
PagI TCATGA 1 cut(s) 592
PctI GAATGC 1 cut(s) 622
PdmI GAANNNNTTC 1 cut(s) 620
PfeI GAWTC 1 cut(s) 436
PfoI TCCNGGA 1 cut(s) 319
PkrI GCNGC 1 cut(s) 540
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
PpuMI RGGWCCY 1 cut(s) 283
Psp5II RGGWCCY 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 319
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 2 cut(s) 201, 285
PspPI GGNCC 2 cut(s) 283, 581
PspPPI RGGWCCY 1 cut(s) 283
RsaI GTAC 1 cut(s) 710
RsaNI GTAC 1 cut(s) 709
RseI CAYNNNNRTG 2 cut(s) 194, 371
SatI GCNGC 1 cut(s) 539
Sau3AI GATC 5 cut(s) 102, 204, 243, 516, 648
Sau96I GGNCC 2 cut(s) 283, 581
SchI GAGTC 1 cut(s) 245
ScrFI CCNGG 3 cut(s) 321, 432, 433
SduI GDGCHC 1 cut(s) 256
SetI ASST 9 cut(s) 135, 149, 264, 285, 406, 486, 564, 586, 714
SfaNI GCATC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 635
SinI GGWCC 2 cut(s) 283, 581
SlaI CTCGAG 1 cut(s) 635
SmaI CCCGGG 1 cut(s) 433
SmiMI CAYNNNNRTG 2 cut(s) 194, 371
SmlI CTYRAG 2 cut(s) 233, 635
SmoI CTYRAG 2 cut(s) 233, 635
Sse9I AATT 4 cut(s) 156, 350, 476, 587
SsiI CCGC 4 cut(s) 22, 39, 67, 97
SspMI CTAG 1 cut(s) 390
StyD4I CCNGG 3 cut(s) 319, 430, 431
StyI CCWWGG 1 cut(s) 732
TaaI ACNGT 1 cut(s) 112
TaiI ACGT 1 cut(s) 264
TaqI TCGA 7 cut(s) 14, 225, 381, 519, 636, 643, 651
TaqII GACCGA 1 cut(s) 520
TasI AATT 4 cut(s) 156, 350, 476, 587
TfiI GAWTC 1 cut(s) 436
TscAI CASTG 3 cut(s) 178, 184, 190
TseI GCWGC 1 cut(s) 538
TspDTI ATGAA 2 cut(s) 149, 581
TspGWI ACGGA 1 cut(s) 52
TspMI CCCGGG 1 cut(s) 431
TspRI CASTG 3 cut(s) 178, 184, 190
VpaK11BI GGWCC 2 cut(s) 283, 581
XapI RAATTY 2 cut(s) 350, 587
XhoI CTCGAG 1 cut(s) 635
XmaI CCCGGG 1 cut(s) 431
XmiI GTMKAC 1 cut(s) 312
XmnI GAANNNNTTC 1 cut(s) 620
XspI CTAG 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.