RLG00000024654

protein At2g39795, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008277
Physical Location & Seq
Forward (+)
37920121 .. 37921288
1168 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024654

Sequence Viewer

Length: 768 bp
ATGGCCTTGTTCTCATCGATGCTTCGCAGGCCATCCTACTCCTTATTCTCCTTCGCGCTCCGCACCGTCGGTTCTACAACAACCTTTCACAGCCGATTCTCCACGGTCCCCTATTTCGGCAGTGAGATCACCCGAGAATGCTCTGTTCCATTTCGGCATTTCTCCGCCGCTACCGCCACCGCCATGAAACCGGCCGCCGACGACAGGGTGGTCGGAGCACTCGAGTCTGAGATCAAGGTCGCCGAAGAGGACATACAAAATTATAAGGTGGGTGAGGTTGCAGATGGGTTTCCTTTTGAAATAGTAGACAATGCTGGAGAGCAGACCATCATGCTCAGAAGAGAATTCGATGGAGAAATCATCAAAGTGGCAGTCGATATTCCTAATGTTTCTTGCACCGAGGACAATGATGATGAGGAGGAGAAGGAGGAGGAGAATGAAGACACTGGGATTCCAATGGTTGTGAGTATTTCGAAAGGAAATGGACAGGAGCTGGAGTTTGGGCTCACTGCCTTTTCTGATGAGATTTGTGTCGATAGCTTGTCGATGAGGCAGCCAGAAGATTCTGAAGGATCGAAACGGATTGCATATGAAGGGCCTAATTTTAGTGACTTGGATGAGAGCTTTCAGAAGGCCTTGCAGAAATATCTGGAGGTTCGAGGGATCAAGTCGAGCATAACTAAGTTCTTGCAGGAGTATATGAGGAACAAAGACTTCAGAGAGTACTTGATGTGGTTGAAGAACCTAAAAGGTTTTATTGAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
MAM33

Protein Analysis

256

Amino Acids

28.86

Weight (kDa)

4.57

Isoelectric Point (pI)

50.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAM33 PF02330 74 - 253 1.2e-44 Mitochondrial glycoprotein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 264
AasI GACNNNNNNGTC 1 cut(s) 209
AccI GTMKAC 1 cut(s) 306
AccII CGCG 1 cut(s) 56
AciI CCGC 6 cut(s) 61, 165, 168, 174, 180, 195
AclWI GGATC 2 cut(s) 580, 671
AcoI YGGCCR 1 cut(s) 192
AcsI RAATTY 1 cut(s) 344
AcuI CTGAAG 2 cut(s) 588, 700
AfaI GTAC 1 cut(s) 725
AfiI CCNNNNNNNGG 2 cut(s) 116, 204
AgsI TTSAA 2 cut(s) 299, 739
AluBI AGCT 3 cut(s) 493, 540, 624
AluI AGCT 3 cut(s) 493, 540, 624
Alw21I GWGCWC 1 cut(s) 220
AlwI GGATC 2 cut(s) 580, 671
AlwNI CAGNNNCTG 1 cut(s) 493
Ama87I CYCGRG 2 cut(s) 132, 221
AoxI GGCC 5 cut(s) 3, 29, 192, 596, 633
ApeKI GCWGC 1 cut(s) 553
ApoI RAATTY 1 cut(s) 344
ArsI GACNNNNNNTTYG 2 cut(s) 357, 389
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 2 cut(s) 106, 596
AsuHPI GGTGA 2 cut(s) 121, 284
AsuII TTCGAA 1 cut(s) 473
AvaI CYCGRG 2 cut(s) 132, 221
AvaII GGWCC 1 cut(s) 106
BanII GRGCYC 1 cut(s) 507
BarI GAAGNNNNNNTAC 2 cut(s) 237, 269
BbsI GAAGAC 1 cut(s) 447
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 1 cut(s) 565
BccI CCATC 4 cut(s) 40, 278, 335, 344
BisI GCNGC 3 cut(s) 168, 195, 554
BlsI GCNGC 3 cut(s) 169, 196, 555
BmcAI AGTACT 1 cut(s) 725
Bme18I GGWCC 1 cut(s) 106
BmeT110I CYCGRG 2 cut(s) 132, 221
BmgT120I GGNCC 2 cut(s) 106, 596
BmiI GGNNCC 1 cut(s) 108
BmrI ACTGGG 1 cut(s) 456
BmsI GCATC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 456
BpiI GAAGAC 1 cut(s) 447
BpmI CTGGAG 3 cut(s) 336, 515, 671
Bpu14I TTCGAA 1 cut(s) 473
Bsa29I ATCGAT 1 cut(s) 17
BsaJI CCNNGG 2 cut(s) 102, 399
BsaXI ACNNNNNCTCC 2 cut(s) 482, 512
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 204
Bse118I RCCGGY 1 cut(s) 190
Bse1I ACTGG 1 cut(s) 451
BseCI ATCGAT 1 cut(s) 17
BseDI CCNNGG 2 cut(s) 102, 399
BseGI GGATG 2 cut(s) 32, 622
BseLI CCNNNNNNNGG 2 cut(s) 116, 204
BseMII CTCAG 2 cut(s) 219, 349
BseNI ACTGG 1 cut(s) 451
BseRI GAGGAG 4 cut(s) 431, 434, 443, 446
BseX3I CGGCCG 1 cut(s) 192
BseXI GCAGC 1 cut(s) 565
Bsh1236I CGCG 1 cut(s) 56
Bsh1285I CGRYCG 1 cut(s) 195
BshFI GGCC 5 cut(s) 5, 31, 194, 598, 635
BshVI ATCGAT 1 cut(s) 17
BsiEI CGRYCG 1 cut(s) 195
BsiHKAI GWGCWC 1 cut(s) 220
BsiHKCI CYCGRG 2 cut(s) 132, 221
BsiSI CCGG 1 cut(s) 191
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 2 cut(s) 116, 204
BsmFI GGGAC 1 cut(s) 92
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 5 cut(s) 5, 31, 194, 598, 635
BsoBI CYCGRG 2 cut(s) 132, 221
Bsp119I TTCGAA 1 cut(s) 473
Bsp1286I GDGCHC 2 cut(s) 220, 507
Bsp143I GATC 4 cut(s) 126, 231, 572, 663
BspACI CCGC 6 cut(s) 61, 165, 168, 174, 180, 195
BspANI GGCC 5 cut(s) 5, 31, 194, 598, 635
BspCNI CTCAG 2 cut(s) 220, 348
BspDI ATCGAT 1 cut(s) 17
BspFNI CGCG 1 cut(s) 56
BspLI GGNNCC 1 cut(s) 108
BspPI GGATC 2 cut(s) 580, 671
BspT104I TTCGAA 1 cut(s) 473
BsrFI RCCGGY 1 cut(s) 190
BsrI ACTGG 1 cut(s) 451
BssAI RCCGGY 1 cut(s) 190
BssECI CCNNGG 2 cut(s) 102, 399
BssMI GATC 4 cut(s) 126, 231, 572, 663
Bst4CI ACNGT 2 cut(s) 67, 106
Bst6I CTCTTC 2 cut(s) 240, 334
BstBI TTCGAA 1 cut(s) 473
BstC8I GCNNGC 1 cut(s) 29
BstDEI CTNAG 3 cut(s) 228, 335, 681
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 2 cut(s) 32, 622
BstFNI CGCG 1 cut(s) 56
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 4 cut(s) 129, 234, 575, 666
BstMBI GATC 4 cut(s) 126, 231, 572, 663
BstMCI CGRYCG 1 cut(s) 195
BstMWI GCNNNNNNNGC 2 cut(s) 28, 173
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 565
BstV2I GAAGAC 1 cut(s) 447
BstZI CGGCCG 1 cut(s) 192
Bsu15I ATCGAT 1 cut(s) 17
BsuRI GGCC 5 cut(s) 5, 31, 194, 598, 635
BsuTUI ATCGAT 1 cut(s) 17
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 2 cut(s) 32, 622
BtsI GCAGTG 2 cut(s) 127, 507
BtsIMutI CAGTG 3 cut(s) 127, 444, 507
Cac8I GCNNGC 1 cut(s) 29
CaiI CAGNNNCTG 1 cut(s) 493
CfoI GCGC 1 cut(s) 58
Cfr10I RCCGGY 1 cut(s) 190
Cfr13I GGNCC 2 cut(s) 106, 596
ClaI ATCGAT 1 cut(s) 17
Csp6I GTAC 1 cut(s) 724
CviAII CATG 2 cut(s) 184, 331
CviQI GTAC 1 cut(s) 724
DdeI CTNAG 3 cut(s) 228, 335, 681
DpnI GATC 4 cut(s) 128, 233, 574, 665
DpnII GATC 4 cut(s) 126, 231, 572, 663
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 1 cut(s) 192
EagI CGGCCG 1 cut(s) 192
Eam1104I CTCTTC 2 cut(s) 240, 334
EarI CTCTTC 2 cut(s) 240, 334
EciI GGCGGA 1 cut(s) 154
EclXI CGGCCG 1 cut(s) 192
Eco147I AGGCCT 1 cut(s) 635
Eco24I GRGCYC 1 cut(s) 507
Eco47I GGWCC 1 cut(s) 106
Eco52I CGGCCG 1 cut(s) 192
Eco57I CTGAAG 2 cut(s) 588, 700
Eco88I CYCGRG 2 cut(s) 132, 221
EcoO109I RGGNCCY 1 cut(s) 596
EcoRI GAATTC 1 cut(s) 344
EcoT38I GRGCYC 1 cut(s) 507
FaeI CATG 2 cut(s) 187, 334
FaiI YATR 9 cut(s) 185, 254, 264, 332, 589, 591, 677, 699, 701
FaqI GGGAC 1 cut(s) 92
FatI CATG 2 cut(s) 183, 330
FauNDI CATATG 1 cut(s) 589
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 3 cut(s) 168, 195, 554
FokI GGATG 2 cut(s) 19, 629
FriOI GRGCYC 1 cut(s) 507
Fsp4HI GCNGC 3 cut(s) 168, 195, 554
GlaI GCGC 1 cut(s) 57
GluI GCNGC 3 cut(s) 168, 195, 554
GsuI CTGGAG 3 cut(s) 336, 515, 671
HaeIII GGCC 5 cut(s) 5, 31, 194, 598, 635
HapII CCGG 1 cut(s) 191
HhaI GCGC 1 cut(s) 58
Hin1II CATG 2 cut(s) 187, 334
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HinfI GANTC 4 cut(s) 96, 224, 451, 563
HpaII CCGG 1 cut(s) 191
HphI GGTGA 2 cut(s) 121, 284
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 7 cut(s) 215, 229, 338, 520, 568, 630, 719
Hpy188III TCNNGA 1 cut(s) 650
Hpy8I GTNNAC 1 cut(s) 307
Hpy99I CGWCG 2 cut(s) 71, 203
HpyAV CCTTC 5 cut(s) 61, 418, 563, 587, 625
HpyCH4III ACNGT 2 cut(s) 67, 106
HpyCH4V TGCA 5 cut(s) 281, 396, 587, 640, 691
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 173
HpyF3I CTNAG 3 cut(s) 228, 335, 681
Hsp92II CATG 2 cut(s) 187, 334
HspAI GCGC 1 cut(s) 56
Kzo9I GATC 4 cut(s) 126, 231, 572, 663
LmnI GCTCC 3 cut(s) 63, 215, 490
Lsp1109I GCAGC 1 cut(s) 565
LweI GCATC 1 cut(s) 9
MaeIII GTNAC 1 cut(s) 608
MalI GATC 4 cut(s) 128, 233, 574, 665
MboI GATC 4 cut(s) 126, 231, 572, 663
MboII GAAGA 5 cut(s) 257, 351, 452, 572, 751
MhlI GDGCHC 2 cut(s) 220, 507
MluCI AATT 3 cut(s) 259, 344, 601
MlyI GAGTC 1 cut(s) 233
MmeI TCCRAC 1 cut(s) 193
MslI CAYNNNNRTG 2 cut(s) 182, 365
MspI CCGG 1 cut(s) 191
Mva1269I GAATGC 1 cut(s) 143
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 2 cut(s) 28, 173
NdeI CATATG 1 cut(s) 589
NdeII GATC 4 cut(s) 126, 231, 572, 663
NlaIII CATG 2 cut(s) 187, 334
NlaIV GGNNCC 1 cut(s) 108
NmuCI GTSAC 1 cut(s) 608
NspV TTCGAA 1 cut(s) 473
PaeR7I CTCGAG 1 cut(s) 221
PceI AGGCCT 1 cut(s) 635
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 1 cut(s) 143
PfeI GAWTC 3 cut(s) 96, 451, 563
PkrI GCNGC 3 cut(s) 169, 196, 555
PleI GAGTC 1 cut(s) 232
PpsI GAGTC 1 cut(s) 232
PsiI TTATAA 1 cut(s) 264
PspN4I GGNNCC 1 cut(s) 108
PspPI GGNCC 2 cut(s) 106, 596
PspXI VCTCGAGB 1 cut(s) 221
PstNI CAGNNNCTG 1 cut(s) 493
RsaI GTAC 1 cut(s) 725
RsaNI GTAC 1 cut(s) 724
RseI CAYNNNNRTG 2 cut(s) 182, 365
SatI GCNGC 3 cut(s) 168, 195, 554
Sau3AI GATC 4 cut(s) 126, 231, 572, 663
Sau96I GGNCC 2 cut(s) 106, 596
ScaI AGTACT 1 cut(s) 725
SchI GAGTC 1 cut(s) 233
SduI GDGCHC 2 cut(s) 220, 507
SfaNI GCATC 1 cut(s) 9
Sfr274I CTCGAG 1 cut(s) 221
SfuI TTCGAA 1 cut(s) 473
SinI GGWCC 1 cut(s) 106
SlaI CTCGAG 1 cut(s) 221
SmiMI CAYNNNNRTG 2 cut(s) 182, 365
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 3 cut(s) 259, 344, 601
SseBI AGGCCT 1 cut(s) 635
SsiI CCGC 6 cut(s) 61, 165, 168, 174, 180, 195
StuI AGGCCT 1 cut(s) 635
TaaI ACNGT 2 cut(s) 67, 106
TasI AATT 3 cut(s) 259, 344, 601
TatI WGTACW 1 cut(s) 723
TauI GCSGC 2 cut(s) 170, 197
TfiI GAWTC 3 cut(s) 96, 451, 563
TscAI CASTG 3 cut(s) 127, 451, 514
TseFI GTSAC 1 cut(s) 608
TseI GCWGC 1 cut(s) 553
Tsp45I GTSAC 1 cut(s) 608
TspDTI ATGAA 3 cut(s) 200, 453, 606
TspGWI ACGGA 1 cut(s) 595
TspRI CASTG 3 cut(s) 127, 451, 514
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 1 cut(s) 344
XhoI CTCGAG 1 cut(s) 221
XmiI GTMKAC 1 cut(s) 306
ZrmI AGTACT 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.