Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0008277

Physical Location & Seq

Genomic Coordinates Forward (+)

23057860 .. 23059649

Gene Structure 1790 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1150500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 744 bp

ATGGCTCTCTTCTCGATGCTCCGCAGGCCGTCTTACTCCGCCCTAGCCCTCGCCGTCCGCTCCGTCCGCGCCGTCGGATCTGCACCCACCTTTCACCGCGCGATCTCCACCGTCCCTGCTTTCAGCCCACAGCTCCTCCGGAGAAGCTTTGTTCCAGTTCATCGATTCTCCACGGCCACGGCCACCGCCGTGGAGCCGATCGGTGAACAAAGTCTGGTCGAAGTCCTTGAGTCCGAGATCAAGTGTGCCCAAGACGTGAAACCCAAAAAAGCCGAGGTCCCAGAGAAATTTCCTTTTGAAATAGTGGACAATCCTGGAGACCAAACCATTCTGCTCAAAAGAGAATTTGAAGGCGAATCCATCCAAGTATGTGTCGATATTCCTAGTTTTTCCGCAGATGATGAGGACGAGTGGGAAGACCCCGGGATTCCGATGGTGGTGAGCATTTCCAAACCAAATGGACTGCAACTGGAGCTTGGTGTTTCTGTCTTTGAAACTGAGATTTCGATCGATAGCTTGTCCGTCAAGCAGCCTGAGCTTTCTTCCAAAGAGCTTGATTATGCTTATGATGGACCTGAATTTCCCGACTTGGATGAGAAGTTGCAGAATGCTTTCTACAAGTATCTCGAGGCTCGAGGGATCAAGCACACCATGGCTGACTTCTTGTATGATTACATGAAGAACAAAAACAACAAAGAGTTCCTGATGTGGTTGAAGAACGTCAAGCGTGCTGTTCAGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko05168 map05168

Pfam Domains

Protein Families

MAM33

Protein Analysis

248

Amino Acids

27.84

Weight (kDa)

4.95

Isoelectric Point (pI)

54.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MAM33	PF02330	76 - 243	1.5e-39	Mitochondrial glycoprotein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008277)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02050
fragaria_vesca	FvH4_6g13000
malus_domestica	MD00G1141400.v1.1 MD12G1150500.v1.1
prunus_persica	Prupe.6G262100_v2.0.a1
pyrus_communis	pycom12g14430
rosa_chinensis	RchiOBHm_Chr3g0465131
rosa_laevigata	RLG00000008900 RLG00000024654
rosa_multiflora	Rmu_sc0012005.1_g000006 Rmu_ssc0000252.1_g000039
rosa_roxburghii	Rroxscaffold_3G00232030 Rroxscaffold_6G00415220
rosa_rugosa	Rorug03G0075700
rosa_samantha	Rh3BG139300 Rh3CG141300 Rh3CG149600 Rh3CG149700 Rh3DG140600 Rh6CG170300 Rh6DG162600 Rh7AG360700
rosa_wichuraiana	Rw3G011400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	60
AccII	CGCG	3 cut(s)	69, 99, 101
AccIII	TCCGGA	1 cut(s)	138
AciI	CCGC	7 cut(s)	22, 39, 58, 67, 97, 186, 393
AclWI	GGATC	2 cut(s)	85, 647
AcoI	YGGCCR	2 cut(s)	174, 180
AcsI	RAATTY	3 cut(s)	287, 344, 578
AgsI	TTSAA	4 cut(s)	299, 350, 494, 715
AjiI	CACGTC	1 cut(s)	256
AjnI	CCWGG	1 cut(s)	313
AleI	CACNNNNGTG	1 cut(s)	188
AluBI	AGCT	6 cut(s)	133, 147, 475, 516, 538, 553
AluI	AGCT	6 cut(s)	133, 147, 475, 516, 538, 553
Alw26I	GTCTC	1 cut(s)	312
AlwI	GGATC	2 cut(s)	85, 647
Ama87I	CYCGRG	3 cut(s)	422, 626, 633
Aor13HI	TCCGGA	1 cut(s)	138
AoxI	GGCC	3 cut(s)	26, 174, 180
ApeKI	GCWGC	1 cut(s)	529
ApoI	RAATTY	3 cut(s)	287, 344, 578
Asp700I	GAANNNNTTC	1 cut(s)	611
AspLEI	GCGC	2 cut(s)	71, 101
AspS9I	GGNCC	2 cut(s)	277, 572
AsuC2I	CCSGG	2 cut(s)	423, 424
AsuHPI	GGTGA	3 cut(s)	86, 215, 451
AvaI	CYCGRG	3 cut(s)	422, 626, 633
AvaII	GGWCC	2 cut(s)	277, 572
BaeGI	GKGCMC	1 cut(s)	250
BbsI	GAAGAC	1 cut(s)	423
BbvI	GCAGC	1 cut(s)	541
BccI	CCATC	3 cut(s)	368, 427, 563
BceAI	ACGGC	6 cut(s)	13, 38, 56, 173, 189, 195
BciT130I	CCWGG	1 cut(s)	315
BcnI	CCSGG	2 cut(s)	423, 424
BcoDI	GTCTC	1 cut(s)	312
BfaI	CTAG	2 cut(s)	44, 384
BisI	GCNGC	1 cut(s)	530
BlsI	GCNGC	1 cut(s)	531
Bme1390I	CCNGG	3 cut(s)	315, 423, 424
Bme18I	GGWCC	2 cut(s)	277, 572
BmeT110I	CYCGRG	3 cut(s)	422, 626, 633
BmgBI	CACGTC	1 cut(s)	256
BmgT120I	GGNCC	2 cut(s)	277, 572
BmiI	GGNNCC	2 cut(s)	195, 279
BmrFI	CCNGG	3 cut(s)	315, 423, 424
BmsI	GCATC	1 cut(s)	6
BpiI	GAAGAC	1 cut(s)	423
BpmI	CTGGAG	2 cut(s)	336, 491
Bpu10I	CCTNAGC	1 cut(s)	534
BpuEI	CTTGAG	1 cut(s)	248
BpuMI	CCSGG	2 cut(s)	423, 424
Bsa29I	ATCGAT	2 cut(s)	163, 510
BsaBI	GATNNNNATC	1 cut(s)	506
BsaI	GGTCTC	1 cut(s)	312
BsaJI	CCNNGG	7 cut(s)	171, 177, 189, 273, 421, 422, 651
BsaWI	WCCGGW	1 cut(s)	138
BsaXI	ACNNNNNCTCC	2 cut(s)	120, 150
Bse1I	ACTGG	2 cut(s)	155, 474
Bse8I	GATNNNNATC	1 cut(s)	506
BseAI	TCCGGA	1 cut(s)	138
BseBI	CCWGG	1 cut(s)	315
BseCI	ATCGAT	2 cut(s)	163, 510
BseDI	CCNNGG	7 cut(s)	171, 177, 189, 273, 421, 422, 651
BseGI	GGATG	2 cut(s)	360, 598
BseJI	GATNNNNATC	1 cut(s)	506
BseMII	CTCAG	2 cut(s)	489, 525
BseNI	ACTGG	2 cut(s)	155, 474
BseRI	GAGGAG	1 cut(s)	125
BseSI	GKGCMC	1 cut(s)	250
BseXI	GCAGC	1 cut(s)	541
BsgI	GTGCAG	1 cut(s)	66
Bsh1236I	CGCG	3 cut(s)	69, 99, 101
Bsh1285I	CGRYCG	2 cut(s)	201, 510
BshFI	GGCC	3 cut(s)	28, 176, 182
BshVI	ATCGAT	2 cut(s)	163, 510
BsiEI	CGRYCG	2 cut(s)	201, 510
BsiHKCI	CYCGRG	3 cut(s)	422, 626, 633
BsiSI	CCGG	2 cut(s)	139, 423
BslFI	GGGAC	2 cut(s)	98, 263
BsmAI	GTCTC	1 cut(s)	312
BsmFI	GGGAC	2 cut(s)	98, 263
BsmI	GAATGC	1 cut(s)	613
BsnI	GGCC	3 cut(s)	28, 176, 182
Bso31I	GGTCTC	1 cut(s)	312
BsoBI	CYCGRG	3 cut(s)	422, 626, 633
Bsp1286I	GDGCHC	1 cut(s)	250
Bsp13I	TCCGGA	1 cut(s)	138
Bsp143I	GATC	6 cut(s)	77, 102, 198, 237, 507, 639
Bsp19I	CCATGG	1 cut(s)	651
BspACI	CCGC	7 cut(s)	22, 39, 58, 67, 97, 186, 393
BspANI	GGCC	3 cut(s)	28, 176, 182
BspCNI	CTCAG	2 cut(s)	490, 526
BspDI	ATCGAT	2 cut(s)	163, 510
BspEI	TCCGGA	1 cut(s)	138
BspFNI	CGCG	3 cut(s)	69, 99, 101
BspLI	GGNNCC	2 cut(s)	195, 279
BspPI	GGATC	2 cut(s)	85, 647
BspTNI	GGTCTC	1 cut(s)	312
BsrBI	CCGCTC	1 cut(s)	60
BsrI	ACTGG	2 cut(s)	155, 474
BssECI	CCNNGG	7 cut(s)	171, 177, 189, 273, 421, 422, 651
BssMI	GATC	6 cut(s)	77, 102, 198, 237, 507, 639
BssT1I	CCWWGG	1 cut(s)	651
Bst2UI	CCWGG	1 cut(s)	315
Bst4CI	ACNGT	1 cut(s)	112
Bst6I	CTCTTC	1 cut(s)	14
BstC8I	GCNNGC	2 cut(s)	26, 729
BstDEI	CTNAG	2 cut(s)	498, 534
BstDSI	CCRYGG	4 cut(s)	171, 177, 189, 651
BstF5I	GGATG	2 cut(s)	360, 598
BstFNI	CGCG	3 cut(s)	69, 99, 101
BstHHI	GCGC	2 cut(s)	71, 101
BstKTI	GATC	6 cut(s)	80, 105, 201, 240, 510, 642
BstMAI	GTCTC	1 cut(s)	312
BstMBI	GATC	6 cut(s)	77, 102, 198, 237, 507, 639
BstMCI	CGRYCG	2 cut(s)	201, 510
BstMWI	GCNNNNNNNGC	4 cut(s)	25, 66, 472, 535
BstNI	CCWGG	1 cut(s)	315
BstSCI	CCNGG	3 cut(s)	313, 421, 422
BstSLI	GKGCMC	1 cut(s)	250
BstUI	CGCG	3 cut(s)	69, 99, 101
BstV1I	GCAGC	1 cut(s)	541
BstV2I	GAAGAC	1 cut(s)	423
BstX2I	RGATCY	1 cut(s)	77
BstXI	CCANNNNNNTGG	1 cut(s)	190
BstYI	RGATCY	1 cut(s)	77
Bsu15I	ATCGAT	2 cut(s)	163, 510
BsuRI	GGCC	3 cut(s)	28, 176, 182
BsuTUI	ATCGAT	2 cut(s)	163, 510
BtgI	CCRYGG	4 cut(s)	171, 177, 189, 651
BtrI	CACGTC	1 cut(s)	256
BtsCI	GGATG	2 cut(s)	360, 598
Cac8I	GCNNGC	2 cut(s)	26, 729
CfoI	GCGC	2 cut(s)	71, 101
Cfr13I	GGNCC	2 cut(s)	277, 572
Cfr9I	CCCGGG	1 cut(s)	422
ClaI	ATCGAT	2 cut(s)	163, 510
CviAII	CATG	2 cut(s)	652, 676
DdeI	CTNAG	2 cut(s)	498, 534
DpnI	GATC	6 cut(s)	79, 104, 200, 239, 509, 641
DpnII	GATC	6 cut(s)	77, 102, 198, 237, 507, 639
EaeI	YGGCCR	2 cut(s)	174, 180
Eam1104I	CTCTTC	1 cut(s)	14
EarI	CTCTTC	1 cut(s)	14
EciI	GGCGGA	1 cut(s)	28
Eco130I	CCWWGG	1 cut(s)	651
Eco31I	GGTCTC	1 cut(s)	312
Eco47I	GGWCC	2 cut(s)	277, 572
Eco88I	CYCGRG	3 cut(s)	422, 626, 633
EcoO109I	RGGNCCY	1 cut(s)	277
EcoRII	CCWGG	1 cut(s)	313
EcoT14I	CCWWGG	1 cut(s)	651
ErhI	CCWWGG	1 cut(s)	651
FaeI	CATG	2 cut(s)	655, 679
FaiI	YATR	6 cut(s)	370, 561, 567, 653, 669, 677
FaqI	GGGAC	2 cut(s)	98, 263
FatI	CATG	2 cut(s)	651, 675
Fnu4HI	GCNGC	1 cut(s)	530
FokI	GGATG	2 cut(s)	347, 605
Fsp4HI	GCNGC	1 cut(s)	530
FspBI	CTAG	2 cut(s)	44, 384
GlaI	GCGC	2 cut(s)	70, 100
GluI	GCNGC	1 cut(s)	530
GsuI	CTGGAG	2 cut(s)	336, 491
HaeIII	GGCC	3 cut(s)	28, 176, 182
HapII	CCGG	2 cut(s)	139, 423
HhaI	GCGC	2 cut(s)	71, 101
Hin1II	CATG	2 cut(s)	655, 679
Hin6I	GCGC	2 cut(s)	69, 99
HinP1I	GCGC	2 cut(s)	69, 99
HindIII	AAGCTT	1 cut(s)	145
HinfI	GANTC	4 cut(s)	165, 230, 356, 427
HpaII	CCGG	2 cut(s)	139, 423
HphI	GGTGA	3 cut(s)	86, 215, 451
Hpy166II	GTNNAC	2 cut(s)	206, 307
Hpy188I	TCNGA	3 cut(s)	77, 235, 432
Hpy188III	TCNNGA	5 cut(s)	13, 139, 584, 626, 703
Hpy8I	GTNNAC	2 cut(s)	206, 307
Hpy99I	CGWCG	1 cut(s)	77
HpyAV	CCTTC	1 cut(s)	344
HpyCH4III	ACNGT	1 cut(s)	112
HpyCH4IV	ACGT	2 cut(s)	255, 720
HpyCH4V	TGCA	3 cut(s)	83, 466, 604
HpyF10VI	GCNNNNNNNGC	4 cut(s)	25, 66, 472, 535
HpyF3I	CTNAG	2 cut(s)	498, 534
HpySE526I	ACGT	2 cut(s)	255, 720
Hsp92II	CATG	2 cut(s)	655, 679
HspAI	GCGC	2 cut(s)	69, 99
Kpn2I	TCCGGA	1 cut(s)	138
Kzo9I	GATC	6 cut(s)	77, 102, 198, 237, 507, 639
LmnI	GCTCC	5 cut(s)	24, 65, 138, 193, 472
Lsp1109I	GCAGC	1 cut(s)	541
LweI	GCATC	1 cut(s)	6
MaeI	CTAG	2 cut(s)	44, 384
MaeII	ACGT	2 cut(s)	255, 720
MalI	GATC	6 cut(s)	79, 104, 200, 239, 509, 641
MbiI	CCGCTC	1 cut(s)	60
MboI	GATC	6 cut(s)	77, 102, 198, 237, 507, 639
MboII	GAAGA	4 cut(s)	428, 534, 691, 727
MflI	RGATCY	1 cut(s)	77
MhlI	GDGCHC	1 cut(s)	250
MluCI	AATT	3 cut(s)	287, 344, 578
MlyI	GAGTC	1 cut(s)	239
MmeI	TCCRAC	1 cut(s)	55
MnlI	CCTC	6 cut(s)	59, 146, 268, 397, 622, 629
MroI	TCCGGA	1 cut(s)	138
MroXI	GAANNNNTTC	1 cut(s)	611
MslI	CAYNNNNRTG	1 cut(s)	188
MspI	CCGG	2 cut(s)	139, 423
MspR9I	CCNGG	3 cut(s)	315, 423, 424
Mva1269I	GAATGC	1 cut(s)	613
MvaI	CCWGG	1 cut(s)	315
MvnI	CGCG	3 cut(s)	69, 99, 101
MwoI	GCNNNNNNNGC	4 cut(s)	25, 66, 472, 535
NciI	CCSGG	2 cut(s)	423, 424
NcoI	CCATGG	1 cut(s)	651
NdeII	GATC	6 cut(s)	77, 102, 198, 237, 507, 639
NlaIII	CATG	2 cut(s)	655, 679
NlaIV	GGNNCC	2 cut(s)	195, 279
NmeAIII	GCCGAG	1 cut(s)	298
OliI	CACNNNNGTG	1 cut(s)	188
PaeR7I	CTCGAG	2 cut(s)	626, 633
PctI	GAATGC	1 cut(s)	613
PdmI	GAANNNNTTC	1 cut(s)	611
PfeI	GAWTC	3 cut(s)	165, 356, 427
PfoI	TCCNGGA	1 cut(s)	313
PkrI	GCNGC	1 cut(s)	531
Ple19I	CGATCG	2 cut(s)	201, 510
PleI	GAGTC	1 cut(s)	238
PpsI	GAGTC	1 cut(s)	238
PpuMI	RGGWCCY	1 cut(s)	277
Psp5II	RGGWCCY	1 cut(s)	277
Psp6I	CCWGG	1 cut(s)	313
PspGI	CCWGG	1 cut(s)	313
PspN4I	GGNNCC	2 cut(s)	195, 279
PspPI	GGNCC	2 cut(s)	277, 572
PspPPI	RGGWCCY	1 cut(s)	277
PspXI	VCTCGAGB	1 cut(s)	633
PsuI	RGATCY	1 cut(s)	77
PvuI	CGATCG	2 cut(s)	201, 510
RseI	CAYNNNNRTG	1 cut(s)	188
SatI	GCNGC	1 cut(s)	530
Sau3AI	GATC	6 cut(s)	77, 102, 198, 237, 507, 639
Sau96I	GGNCC	2 cut(s)	277, 572
SchI	GAGTC	1 cut(s)	239
ScrFI	CCNGG	3 cut(s)	315, 423, 424
SduI	GDGCHC	1 cut(s)	250
SfaNI	GCATC	1 cut(s)	6
Sfr274I	CTCGAG	2 cut(s)	626, 633
SinI	GGWCC	2 cut(s)	277, 572
SlaI	CTCGAG	2 cut(s)	626, 633
SmaI	CCCGGG	1 cut(s)	424
SmiMI	CAYNNNNRTG	1 cut(s)	188
SmlI	CTYRAG	3 cut(s)	227, 626, 633
SmoI	CTYRAG	3 cut(s)	227, 626, 633
Sse9I	AATT	3 cut(s)	287, 344, 578
SsiI	CCGC	7 cut(s)	22, 39, 58, 67, 97, 186, 393
SspMI	CTAG	2 cut(s)	44, 384
StyD4I	CCNGG	3 cut(s)	313, 421, 422
StyI	CCWWGG	1 cut(s)	651
TaaI	ACNGT	1 cut(s)	112
TaiI	ACGT	2 cut(s)	258, 723
TaqI	TCGA	8 cut(s)	14, 163, 219, 375, 506, 510, 627, 634
TasI	AATT	3 cut(s)	287, 344, 578
TfiI	GAWTC	3 cut(s)	165, 356, 427
TseI	GCWGC	1 cut(s)	529
TspDTI	ATGAA	2 cut(s)	149, 692
TspGWI	ACGGA	2 cut(s)	52, 511
TspMI	CCCGGG	1 cut(s)	422
VpaK11BI	GGWCC	2 cut(s)	277, 572
XapI	RAATTY	3 cut(s)	287, 344, 578
XhoI	CTCGAG	2 cut(s)	626, 633
XmaI	CCCGGG	1 cut(s)	422
XmnI	GAANNNNTTC	1 cut(s)	611
XspI	CTAG	2 cut(s)	44, 384

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.