pycom12g14430

protein At2g39795, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008277
Physical Location & Seq
Forward (+)
16970005 .. 16971589
1585 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g14430.1

Sequence Viewer

Length: 813 bp
ATGCACAAAACCCTGCTGAAATTGCAAAACCCTAAACCAACTGAATTTTCAGCCATAGCCGGACCTCAAATGGCTCTCTTCTCGATGCTCCGCAGGCCATCCTACTCCGCCCTAGCCCTCGCCGTCCGCTCCATCCGCGCCGTCGGATCTGCACCCACCTTTCACCGCTCGATCTCCACCGTCCCTGCTTTCAGCCCACAGCTCCTCCGGAGAGGCTTTGTTCCAGTTCATCGATTCTCCACGGCCACGGCCACCGCCGTGGAGCCGATCGGCGAACAAAGTCTGGTCGAAGTCCTTGAGTCCGAGATCAAGTGTGCCCTTGACGTGAAACCCAGAAAAGCCGAGGTCCCAGAAAAGTTTCCTTTTGAAATAGTGGACAATCCTGGAGACCAAACCATTCTGCTCAAAAGAGAATTTGAAGGCGAATCCATCCAAGTATGTGTCGATACTCCTAGTTTTTCCACAGATGATGAGGACGAGTGGGAAGACCCTGGGATTCCGATGGTTGTGAGCATTTCCAAACCAAATGGACTGCAACTGGAGCTTGGTGTTTCTGTCTTTGAAACTGAGATTTCAATCGATAGCTTGTCCGTCAAGCAGCCTGAGCTTTCTTCCAAAGAGCTTGATTATGCTTATGATGGACCTGAATTTCCCGACTTGGATGAGAAGTTGCAGAATGCTTTCTACAAGTATCTCGAGGTTCGAGGGATCAAGCACACCATGGCTGACTTCTTGTATGATTACATGAGGAACAAAAACAACAAAGAGTTCCTGATGTGGTTGAAGAACGTCAAGCGTGTTGTTCAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
MAM33

Protein Analysis

271

Amino Acids

30.49

Weight (kDa)

5.2

Isoelectric Point (pI)

54.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAM33 PF02330 99 - 267 5.8e-38 Mitochondrial glycoprotein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 129, 168
AccII CGCG 1 cut(s) 138
AccIII TCCGGA 1 cut(s) 207
AciI CCGC 6 cut(s) 91, 108, 127, 136, 166, 255
AclWI GGATC 2 cut(s) 154, 716
AcoI YGGCCR 2 cut(s) 243, 249
AcsI RAATTY 3 cut(s) 44, 413, 647
AgsI TTSAA 5 cut(s) 368, 419, 563, 576, 784
AjiI CACGTC 1 cut(s) 325
AjnI CCWGG 2 cut(s) 382, 490
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 63
AleI CACNNNNGTG 1 cut(s) 257
AluBI AGCT 5 cut(s) 202, 544, 585, 607, 622
AluI AGCT 5 cut(s) 202, 544, 585, 607, 622
Alw26I GTCTC 1 cut(s) 381
AlwI GGATC 2 cut(s) 154, 716
Ama87I CYCGRG 1 cut(s) 695
Aor13HI TCCGGA 1 cut(s) 207
AoxI GGCC 3 cut(s) 95, 243, 249
ApeKI GCWGC 1 cut(s) 598
ApoI RAATTY 3 cut(s) 44, 413, 647
Asp700I GAANNNNTTC 2 cut(s) 357, 680
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 3 cut(s) 62, 346, 641
AsuHPI GGTGA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 695
AvaII GGWCC 3 cut(s) 62, 346, 641
BaeGI GKGCMC 1 cut(s) 319
BbsI GAAGAC 1 cut(s) 492
BbvI GCAGC 1 cut(s) 610
BccI CCATC 5 cut(s) 106, 140, 437, 496, 632
BceAI ACGGC 5 cut(s) 107, 125, 242, 258, 264
BciT130I CCWGG 2 cut(s) 384, 492
BcoDI GTCTC 1 cut(s) 381
BfaI CTAG 2 cut(s) 113, 453
BisI GCNGC 1 cut(s) 599
BlsI GCNGC 1 cut(s) 600
Bme1390I CCNGG 2 cut(s) 384, 492
Bme18I GGWCC 3 cut(s) 62, 346, 641
BmeT110I CYCGRG 1 cut(s) 695
BmgBI CACGTC 1 cut(s) 325
BmgT120I GGNCC 3 cut(s) 62, 346, 641
BmiI GGNNCC 2 cut(s) 264, 348
BmrFI CCNGG 2 cut(s) 384, 492
BmsI GCATC 1 cut(s) 75
BpiI GAAGAC 1 cut(s) 492
BpmI CTGGAG 2 cut(s) 405, 560
Bpu10I CCTNAGC 1 cut(s) 603
BpuEI CTTGAG 1 cut(s) 317
Bsa29I ATCGAT 2 cut(s) 232, 579
BsaBI GATNNNNATC 1 cut(s) 575
BsaI GGTCTC 1 cut(s) 381
BsaJI CCNNGG 7 cut(s) 240, 246, 258, 342, 490, 491, 720
BsaWI WCCGGW 1 cut(s) 207
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bse1I ACTGG 2 cut(s) 224, 543
Bse8I GATNNNNATC 1 cut(s) 575
BseAI TCCGGA 1 cut(s) 207
BseBI CCWGG 2 cut(s) 384, 492
BseCI ATCGAT 2 cut(s) 232, 579
BseDI CCNNGG 7 cut(s) 240, 246, 258, 342, 490, 491, 720
BseGI GGATG 4 cut(s) 98, 132, 429, 667
BseJI GATNNNNATC 1 cut(s) 575
BseMII CTCAG 2 cut(s) 558, 594
BseNI ACTGG 2 cut(s) 224, 543
BseRI GAGGAG 1 cut(s) 194
BseSI GKGCMC 1 cut(s) 319
BseXI GCAGC 1 cut(s) 610
BsgI GTGCAG 1 cut(s) 135
Bsh1236I CGCG 1 cut(s) 138
Bsh1285I CGRYCG 1 cut(s) 270
BshFI GGCC 3 cut(s) 97, 245, 251
BshVI ATCGAT 2 cut(s) 232, 579
BsiEI CGRYCG 1 cut(s) 270
BsiHKCI CYCGRG 1 cut(s) 695
BsiSI CCGG 2 cut(s) 60, 208
BslFI GGGAC 2 cut(s) 167, 332
BsmAI GTCTC 1 cut(s) 381
BsmFI GGGAC 2 cut(s) 167, 332
BsmI GAATGC 1 cut(s) 682
BsnI GGCC 3 cut(s) 97, 245, 251
Bso31I GGTCTC 1 cut(s) 381
BsoBI CYCGRG 1 cut(s) 695
Bsp1286I GDGCHC 1 cut(s) 319
Bsp13I TCCGGA 1 cut(s) 207
Bsp143I GATC 5 cut(s) 146, 171, 267, 306, 708
Bsp19I CCATGG 1 cut(s) 720
BspACI CCGC 6 cut(s) 91, 108, 127, 136, 166, 255
BspANI GGCC 3 cut(s) 97, 245, 251
BspCNI CTCAG 2 cut(s) 559, 595
BspDI ATCGAT 2 cut(s) 232, 579
BspEI TCCGGA 1 cut(s) 207
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 2 cut(s) 264, 348
BspPI GGATC 2 cut(s) 154, 716
BspTNI GGTCTC 1 cut(s) 381
BsrBI CCGCTC 2 cut(s) 129, 168
BsrI ACTGG 2 cut(s) 224, 543
BssECI CCNNGG 7 cut(s) 240, 246, 258, 342, 490, 491, 720
BssMI GATC 5 cut(s) 146, 171, 267, 306, 708
BssT1I CCWWGG 1 cut(s) 720
Bst2UI CCWGG 2 cut(s) 384, 492
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 1 cut(s) 83
BstC8I GCNNGC 1 cut(s) 95
BstDEI CTNAG 2 cut(s) 567, 603
BstDSI CCRYGG 4 cut(s) 240, 246, 258, 720
BstF5I GGATG 4 cut(s) 98, 132, 429, 667
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 5 cut(s) 149, 174, 270, 309, 711
BstMAI GTCTC 1 cut(s) 381
BstMBI GATC 5 cut(s) 146, 171, 267, 306, 708
BstMCI CGRYCG 1 cut(s) 270
BstMWI GCNNNNNNNGC 5 cut(s) 22, 94, 135, 541, 604
BstNI CCWGG 2 cut(s) 384, 492
BstSCI CCNGG 2 cut(s) 382, 490
BstSLI GKGCMC 1 cut(s) 319
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 1 cut(s) 610
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 1 cut(s) 146
BstXI CCANNNNNNTGG 1 cut(s) 259
BstYI RGATCY 1 cut(s) 146
Bsu15I ATCGAT 2 cut(s) 232, 579
BsuRI GGCC 3 cut(s) 97, 245, 251
BsuTUI ATCGAT 2 cut(s) 232, 579
BtgI CCRYGG 4 cut(s) 240, 246, 258, 720
BtrI CACGTC 1 cut(s) 325
BtsCI GGATG 4 cut(s) 98, 132, 429, 667
Cac8I GCNNGC 1 cut(s) 95
CfoI GCGC 1 cut(s) 140
Cfr13I GGNCC 3 cut(s) 62, 346, 641
ClaI ATCGAT 2 cut(s) 232, 579
CviAII CATG 2 cut(s) 721, 745
DdeI CTNAG 2 cut(s) 567, 603
DpnI GATC 5 cut(s) 148, 173, 269, 308, 710
DpnII GATC 5 cut(s) 146, 171, 267, 306, 708
EaeI YGGCCR 2 cut(s) 243, 249
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
EciI GGCGGA 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 720
Eco31I GGTCTC 1 cut(s) 381
Eco47I GGWCC 3 cut(s) 62, 346, 641
Eco88I CYCGRG 1 cut(s) 695
EcoO109I RGGNCCY 1 cut(s) 346
EcoRII CCWGG 2 cut(s) 382, 490
EcoT14I CCWWGG 1 cut(s) 720
ErhI CCWWGG 1 cut(s) 720
FaeI CATG 2 cut(s) 724, 748
FaiI YATR 7 cut(s) 56, 439, 630, 636, 722, 738, 746
FaqI GGGAC 2 cut(s) 167, 332
FatI CATG 2 cut(s) 720, 744
Fnu4HI GCNGC 1 cut(s) 599
FokI GGATG 4 cut(s) 85, 119, 416, 674
Fsp4HI GCNGC 1 cut(s) 599
FspBI CTAG 2 cut(s) 113, 453
GlaI GCGC 1 cut(s) 139
GluI GCNGC 1 cut(s) 599
GsuI CTGGAG 2 cut(s) 405, 560
HaeIII GGCC 3 cut(s) 97, 245, 251
HapII CCGG 2 cut(s) 60, 208
HhaI GCGC 1 cut(s) 140
Hin1II CATG 2 cut(s) 724, 748
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HinfI GANTC 4 cut(s) 234, 299, 425, 496
HpaII CCGG 2 cut(s) 60, 208
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 3 cut(s) 146, 304, 501
Hpy188III TCNNGA 5 cut(s) 82, 208, 653, 695, 772
Hpy8I GTNNAC 1 cut(s) 376
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 413
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4IV ACGT 2 cut(s) 324, 789
HpyCH4V TGCA 5 cut(s) 4, 25, 152, 535, 673
HpyF10VI GCNNNNNNNGC 5 cut(s) 22, 94, 135, 541, 604
HpyF3I CTNAG 2 cut(s) 567, 603
HpySE526I ACGT 2 cut(s) 324, 789
Hsp92II CATG 2 cut(s) 724, 748
HspAI GCGC 1 cut(s) 138
Kpn2I TCCGGA 1 cut(s) 207
Kzo9I GATC 5 cut(s) 146, 171, 267, 306, 708
LmnI GCTCC 5 cut(s) 93, 134, 207, 262, 541
Lsp1109I GCAGC 1 cut(s) 610
LweI GCATC 1 cut(s) 75
MaeI CTAG 2 cut(s) 113, 453
MaeII ACGT 2 cut(s) 324, 789
MalI GATC 5 cut(s) 148, 173, 269, 308, 710
MbiI CCGCTC 2 cut(s) 129, 168
MboI GATC 5 cut(s) 146, 171, 267, 306, 708
MboII GAAGA 4 cut(s) 70, 497, 603, 796
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 1 cut(s) 319
MluCI AATT 4 cut(s) 20, 44, 413, 647
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 124
MnlI CCTC 9 cut(s) 75, 128, 206, 215, 337, 466, 691, 698, 741
MroI TCCGGA 1 cut(s) 207
MroXI GAANNNNTTC 2 cut(s) 357, 680
MslI CAYNNNNRTG 1 cut(s) 257
MspI CCGG 2 cut(s) 60, 208
MspR9I CCNGG 2 cut(s) 384, 492
Mva1269I GAATGC 1 cut(s) 682
MvaI CCWGG 2 cut(s) 384, 492
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 5 cut(s) 22, 94, 135, 541, 604
NcoI CCATGG 1 cut(s) 720
NdeII GATC 5 cut(s) 146, 171, 267, 306, 708
NlaIII CATG 2 cut(s) 724, 748
NlaIV GGNNCC 2 cut(s) 264, 348
NmeAIII GCCGAG 1 cut(s) 367
OliI CACNNNNGTG 1 cut(s) 257
PaeR7I CTCGAG 1 cut(s) 695
PasI CCCWGGG 1 cut(s) 491
PctI GAATGC 1 cut(s) 682
PdmI GAANNNNTTC 2 cut(s) 357, 680
PfeI GAWTC 3 cut(s) 234, 425, 496
PfoI TCCNGGA 1 cut(s) 382
PkrI GCNGC 1 cut(s) 600
Ple19I CGATCG 1 cut(s) 270
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
PpuMI RGGWCCY 1 cut(s) 346
Psp5II RGGWCCY 1 cut(s) 346
Psp6I CCWGG 2 cut(s) 382, 490
PspGI CCWGG 2 cut(s) 382, 490
PspN4I GGNNCC 2 cut(s) 264, 348
PspPI GGNCC 3 cut(s) 62, 346, 641
PspPPI RGGWCCY 1 cut(s) 346
PsuI RGATCY 1 cut(s) 146
PvuI CGATCG 1 cut(s) 270
RseI CAYNNNNRTG 1 cut(s) 257
SatI GCNGC 1 cut(s) 599
Sau3AI GATC 5 cut(s) 146, 171, 267, 306, 708
Sau96I GGNCC 3 cut(s) 62, 346, 641
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 2 cut(s) 384, 492
SduI GDGCHC 1 cut(s) 319
SfaNI GCATC 1 cut(s) 75
Sfr274I CTCGAG 1 cut(s) 695
SinI GGWCC 3 cut(s) 62, 346, 641
SlaI CTCGAG 1 cut(s) 695
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 2 cut(s) 296, 695
SmoI CTYRAG 2 cut(s) 296, 695
Sse9I AATT 4 cut(s) 20, 44, 413, 647
SsiI CCGC 6 cut(s) 91, 108, 127, 136, 166, 255
SspMI CTAG 2 cut(s) 113, 453
StyD4I CCNGG 2 cut(s) 382, 490
StyI CCWWGG 1 cut(s) 720
TaaI ACNGT 1 cut(s) 181
TaiI ACGT 2 cut(s) 327, 792
TaqI TCGA 8 cut(s) 83, 170, 232, 288, 444, 579, 696, 703
TasI AATT 4 cut(s) 20, 44, 413, 647
TfiI GAWTC 3 cut(s) 234, 425, 496
TseI GCWGC 1 cut(s) 598
TspDTI ATGAA 1 cut(s) 218
TspGWI ACGGA 1 cut(s) 580
VpaK11BI GGWCC 3 cut(s) 62, 346, 641
XapI RAATTY 3 cut(s) 44, 413, 647
XhoI CTCGAG 1 cut(s) 695
XmnI GAANNNNTTC 2 cut(s) 357, 680
XspI CTAG 2 cut(s) 113, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.