FvH4_6g17980

fructose-bisphosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001206
Physical Location & Seq
Forward (+)
11787930 .. 11796836
8907 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g17980.t8

Sequence Viewer

Length: 1020 bp
ATGACTGCTTACAGAGGCAAATACGCTGATGAGCTCATTAAGAATGCTGCCTACATTGGCACCCCTGGAAAGGGTATTCTAGCTGCTGATGAGTCCACTGGCACTATTGGAAAACGTTTTGACAGCATCAAAGTTGAGAATGTTGAAGAGAACAGGCGTGCTCTTCGTGAGCTCCTCTTCACTGCCCCTGGTGTCCTCCAGTACCTTAGTGGAGTGATCCTCTTTGAGGAGACCCTCTACCAGAAGACAGCTGAAGGCAAGCCTTTCGTTGATGTCTTGAACGAGGGTGGTGTTCTTCCTGGTATCAAGGTTGACAAGGGTGTTGTTGAGCTTAAAGGTACTGATGGTGAGACCACAACCCAGGGATTGGATGGCCTTGGTGCCCGCTGCGCTAAGTACTACGAAGCTGGTGCCAGGTTTGCCAAATGGCGTGCCGTTCTCAAGATTGGTCCCAATGAGCCATCTCAGCTCTCAATCAACGAGAATGCTAATGGCTTGGCTCGTTATGCTATCATCTGCCAGGAGAATGGTTTGGTACCCATTGTTGAGCCTGAGATCCTTGTTGATGGACCCCACTCCATTGACAAGTGTGCTGATGTGACTGAGCGTGTACTTGCTGCATGTTACAAGGCTCTCAATGACCACCACGTCCTTCTTGAAGGAACTCTCTTGAAGCCCAACATGGTTACTCCCGGATCTGAAGCCAAGAAGGCTTCACCAGAAGTGATTGCTGAGTACACCGTCCGTGCCTTGCAGCGCACTACCCCCGCAGCTGTCCCTGCTGTGGTGTTCTTGTCTGGTGGACAGAGTGAGGAGGAGGCTACCCTCAACCTTAACGCCATGAACCAGCTCAAGGGAAAGAAGCCATGGACTCTTTCCTTCTCTTTTGGCCGTGCTCTTCAGGCACTCCTCACAAGGGCCAAGGCCAACTCAGAGGCAACCTTGGGAATCTACAAGGGTGATGCCAAGCTCGGAGAAGGAGCTGCTGAGAGCCTTCATGTCAAGGACTACAAGTACTGA

Protein Analysis

340

Amino Acids

36.5

Weight (kDa)

6.19

Isoelectric Point (pI)

24.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycolytic PF00274 11 - 260 2e-126 Fructose-bisphosphate aldolase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 647
Acc65I GGTACC 1 cut(s) 535
AccB1I GGYRCC 4 cut(s) 59, 380, 410, 535
AccB7I CCANNNNNTGG 1 cut(s) 367
AciI CCGC 2 cut(s) 385, 768
AclI AACGTT 1 cut(s) 115
AclWI GGATC 3 cut(s) 211, 550, 703
AcoI YGGCCR 1 cut(s) 889
AcuI CTGAAG 3 cut(s) 273, 720, 884
AfaI GTAC 7 cut(s) 203, 340, 398, 537, 612, 737, 1016
AfiI CCNNNNNNNGG 7 cut(s) 70, 71, 226, 367, 784, 853, 916
AgsI TTSAA 4 cut(s) 146, 280, 659, 673
AjiI CACGTC 1 cut(s) 649
AjnI CCWGG 6 cut(s) 64, 187, 298, 360, 413, 519
Alw21I GWGCWC 4 cut(s) 36, 163, 174, 898
Alw26I GTCTC 2 cut(s) 224, 344
AlwI GGATC 3 cut(s) 211, 550, 703
AoxI GGCC 4 cut(s) 373, 889, 918, 924
ApeKI GCWGC 7 cut(s) 47, 83, 387, 617, 754, 770, 983
Asp718I GGTACC 1 cut(s) 535
AspLEI GCGC 2 cut(s) 392, 759
AspS9I GGNCC 3 cut(s) 449, 569, 918
AsuC2I CCSGG 1 cut(s) 693
AsuHPI GGTGA 3 cut(s) 359, 708, 971
AvaII GGWCC 2 cut(s) 449, 569
BaeGI GKGCMC 1 cut(s) 385
BanI GGYRCC 4 cut(s) 59, 380, 410, 535
BanII GRGCYC 2 cut(s) 36, 174
BbsI GAAGAC 1 cut(s) 251
Bbv12I GWGCWC 4 cut(s) 36, 163, 174, 898
BbvI GCAGC 7 cut(s) 34, 70, 374, 604, 766, 782, 970
BccI CCATC 4 cut(s) 338, 365, 469, 560
BceAI ACGGC 2 cut(s) 419, 876
BcgI CGANNNNNNTGC 4 cut(s) 247, 281, 392, 426
BciT130I CCWGG 6 cut(s) 66, 189, 300, 362, 415, 521
BcnI CCSGG 1 cut(s) 693
BcoDI GTCTC 2 cut(s) 224, 344
BfaI CTAG 1 cut(s) 80
BglI GCCNNNNNGGC 1 cut(s) 710
BisI GCNGC 7 cut(s) 48, 84, 388, 618, 755, 771, 984
BlsI GCNGC 7 cut(s) 49, 85, 389, 619, 756, 772, 985
BmcAI AGTACT 2 cut(s) 398, 1016
Bme1390I CCNGG 7 cut(s) 66, 189, 300, 362, 415, 521, 693
Bme18I GGWCC 2 cut(s) 449, 569
BmgBI CACGTC 1 cut(s) 649
BmgT120I GGNCC 3 cut(s) 449, 569, 918
BmiI GGNNCC 6 cut(s) 61, 382, 412, 451, 537, 571
BmrFI CCNGG 7 cut(s) 66, 189, 300, 362, 415, 521, 693
BmsI GCATC 2 cut(s) 135, 952
BpiI GAAGAC 1 cut(s) 251
BplI GAGNNNNNCTC 2 cut(s) 204, 236
BpmI CTGGAG 1 cut(s) 182
BpuEI CTTGAG 2 cut(s) 425, 836
BpuMI CCSGG 1 cut(s) 693
BsaI GGTCTC 2 cut(s) 224, 344
BsaJI CCNNGG 8 cut(s) 64, 187, 360, 361, 376, 866, 921, 942
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 71, 226, 367, 784, 853, 916
Bse1I ACTGG 2 cut(s) 103, 199
BseBI CCWGG 6 cut(s) 66, 189, 300, 362, 415, 521
BseDI CCNNGG 8 cut(s) 64, 187, 360, 361, 376, 866, 921, 942
BseGI GGATG 1 cut(s) 376
BseLI CCNNNNNNNGG 7 cut(s) 70, 71, 226, 367, 784, 853, 916
BseMII CTCAG 6 cut(s) 479, 543, 594, 723, 945, 978
BseNI ACTGG 2 cut(s) 103, 199
BseRI GAGGAG 5 cut(s) 164, 242, 827, 830, 899
BseSI GKGCMC 1 cut(s) 385
BseXI GCAGC 7 cut(s) 34, 70, 374, 604, 766, 782, 970
BshFI GGCC 4 cut(s) 375, 891, 920, 926
BshNI GGYRCC 4 cut(s) 59, 380, 410, 535
BsiHKAI GWGCWC 4 cut(s) 36, 163, 174, 898
BsiSI CCGG 1 cut(s) 693
BslFI GGGAC 2 cut(s) 435, 761
BslI CCNNNNNNNGG 7 cut(s) 70, 71, 226, 367, 784, 853, 916
BsmAI GTCTC 2 cut(s) 224, 344
BsmFI GGGAC 2 cut(s) 435, 761
BsmI GAATGC 2 cut(s) 49, 490
BsnI GGCC 4 cut(s) 375, 891, 920, 926
Bso31I GGTCTC 2 cut(s) 224, 344
Bsp1286I GDGCHC 5 cut(s) 36, 163, 174, 385, 898
Bsp143I GATC 3 cut(s) 216, 555, 695
Bsp19I CCATGG 1 cut(s) 866
BspACI CCGC 2 cut(s) 385, 768
BspANI GGCC 4 cut(s) 375, 891, 920, 926
BspCNI CTCAG 6 cut(s) 478, 544, 595, 724, 944, 979
BspLI GGNNCC 6 cut(s) 61, 382, 412, 451, 537, 571
BspPI GGATC 3 cut(s) 211, 550, 703
BspQI GCTCTTC 2 cut(s) 168, 903
BspT107I GGYRCC 4 cut(s) 59, 380, 410, 535
BspTNI GGTCTC 2 cut(s) 224, 344
BsrI ACTGG 2 cut(s) 103, 199
BssECI CCNNGG 8 cut(s) 64, 187, 360, 361, 376, 866, 921, 942
BssMI GATC 3 cut(s) 216, 555, 695
BssT1I CCWWGG 4 cut(s) 376, 866, 921, 942
Bst2UI CCWGG 6 cut(s) 66, 189, 300, 362, 415, 521
Bst4CI ACNGT 1 cut(s) 742
Bst6I CTCTTC 4 cut(s) 141, 168, 182, 903
BstC8I GCNNGC 4 cut(s) 159, 260, 385, 432
BstDEI CTNAG 8 cut(s) 206, 393, 465, 552, 603, 732, 931, 987
BstDSI CCRYGG 1 cut(s) 866
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 1 cut(s) 376
BstHHI GCGC 2 cut(s) 392, 759
BstKTI GATC 3 cut(s) 219, 558, 698
BstMAI GTCTC 2 cut(s) 224, 344
BstMBI GATC 3 cut(s) 216, 555, 695
BstMWI GCNNNNNNNGC 7 cut(s) 389, 419, 466, 506, 710, 779, 902
BstNI CCWGG 6 cut(s) 66, 189, 300, 362, 415, 521
BstNSI RCATGY 1 cut(s) 624
BstSCI CCNGG 7 cut(s) 64, 187, 298, 360, 413, 519, 691
BstSLI GKGCMC 1 cut(s) 385
BstV1I GCAGC 7 cut(s) 34, 70, 374, 604, 766, 782, 970
BstV2I GAAGAC 1 cut(s) 251
BstX2I RGATCY 2 cut(s) 555, 695
BstXI CCANNNNNNTGG 1 cut(s) 527
BstYI RGATCY 2 cut(s) 555, 695
BsuRI GGCC 4 cut(s) 375, 891, 920, 926
BtgI CCRYGG 1 cut(s) 866
BtrI CACGTC 1 cut(s) 649
BtsCI GGATG 1 cut(s) 376
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 2 cut(s) 96, 180
Cac8I GCNNGC 4 cut(s) 159, 260, 385, 432
CfoI GCGC 2 cut(s) 392, 759
Cfr13I GGNCC 3 cut(s) 449, 569, 918
Csp6I GTAC 7 cut(s) 202, 339, 397, 536, 611, 736, 1015
CviAII CATG 5 cut(s) 621, 682, 841, 867, 998
CviQI GTAC 7 cut(s) 202, 339, 397, 536, 611, 736, 1015
DdeI CTNAG 8 cut(s) 206, 393, 465, 552, 603, 732, 931, 987
DpnI GATC 3 cut(s) 218, 557, 697
DpnII GATC 3 cut(s) 216, 555, 695
DrdI GACNNNNNNGTC 1 cut(s) 647
DseDI GACNNNNNNGTC 1 cut(s) 647
EaeI YGGCCR 1 cut(s) 889
Eam1104I CTCTTC 4 cut(s) 141, 168, 182, 903
EarI CTCTTC 4 cut(s) 141, 168, 182, 903
Ecl136II GAGCTC 2 cut(s) 34, 172
Eco130I CCWWGG 4 cut(s) 376, 866, 921, 942
Eco24I GRGCYC 2 cut(s) 36, 174
Eco31I GGTCTC 2 cut(s) 224, 344
Eco47I GGWCC 2 cut(s) 449, 569
Eco53kI GAGCTC 2 cut(s) 34, 172
Eco57I CTGAAG 3 cut(s) 273, 720, 884
EcoICRI GAGCTC 2 cut(s) 34, 172
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoRII CCWGG 6 cut(s) 64, 187, 298, 360, 413, 519
EcoT14I CCWWGG 4 cut(s) 376, 866, 921, 942
EcoT38I GRGCYC 2 cut(s) 36, 174
ErhI CCWWGG 4 cut(s) 376, 866, 921, 942
FaeI CATG 5 cut(s) 624, 685, 844, 870, 1001
FaiI YATR 6 cut(s) 507, 622, 683, 842, 868, 999
FaqI GGGAC 2 cut(s) 435, 761
FatI CATG 5 cut(s) 620, 681, 840, 866, 997
FauI CCCGC 2 cut(s) 392, 775
Fnu4HI GCNGC 7 cut(s) 48, 84, 388, 618, 755, 771, 984
FokI GGATG 1 cut(s) 383
FriOI GRGCYC 2 cut(s) 36, 174
Fsp4HI GCNGC 7 cut(s) 48, 84, 388, 618, 755, 771, 984
FspBI CTAG 1 cut(s) 80
GlaI GCGC 2 cut(s) 391, 758
GluI GCNGC 7 cut(s) 48, 84, 388, 618, 755, 771, 984
GsuI CTGGAG 1 cut(s) 182
HaeIII GGCC 4 cut(s) 375, 891, 920, 926
HapII CCGG 1 cut(s) 693
HhaI GCGC 2 cut(s) 392, 759
Hin1II CATG 5 cut(s) 624, 685, 844, 870, 1001
Hin6I GCGC 2 cut(s) 390, 757
HinP1I GCGC 2 cut(s) 390, 757
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 3 cut(s) 92, 871, 948
HpaII CCGG 1 cut(s) 693
HphI GGTGA 3 cut(s) 359, 708, 971
Hpy166II GTNNAC 5 cut(s) 96, 313, 611, 738, 803
Hpy188I TCNGA 3 cut(s) 700, 934, 974
Hpy188III TCNNGA 5 cut(s) 167, 277, 442, 656, 670
Hpy8I GTNNAC 5 cut(s) 96, 313, 611, 738, 803
HpyAV CCTTC 7 cut(s) 248, 653, 662, 703, 889, 971, 1004
HpyCH4III ACNGT 1 cut(s) 742
HpyCH4IV ACGT 2 cut(s) 115, 648
HpyCH4V TGCA 2 cut(s) 620, 754
HpyF10VI GCNNNNNNNGC 7 cut(s) 389, 419, 466, 506, 710, 779, 902
HpyF3I CTNAG 8 cut(s) 206, 393, 465, 552, 603, 732, 931, 987
HpySE526I ACGT 2 cut(s) 115, 648
Hsp92II CATG 5 cut(s) 624, 685, 844, 870, 1001
HspAI GCGC 2 cut(s) 390, 757
KpnI GGTACC 1 cut(s) 539
Kzo9I GATC 3 cut(s) 216, 555, 695
LguI GCTCTTC 2 cut(s) 168, 903
LmnI GCTCC 2 cut(s) 177, 980
Lsp1109I GCAGC 7 cut(s) 34, 70, 374, 604, 766, 782, 970
LweI GCATC 2 cut(s) 135, 952
MaeI CTAG 1 cut(s) 80
MaeII ACGT 2 cut(s) 115, 648
MaeIII GTNAC 3 cut(s) 598, 623, 685
MalI GATC 3 cut(s) 218, 557, 697
MboI GATC 3 cut(s) 216, 555, 695
MboII GAAGA 6 cut(s) 155, 158, 169, 256, 287, 890
MflI RGATCY 2 cut(s) 555, 695
MhlI GDGCHC 5 cut(s) 36, 163, 174, 385, 898
MlyI GAGTC 2 cut(s) 101, 865
MseI TTAA 3 cut(s) 39, 333, 834
MspA1I CMGCKG 3 cut(s) 251, 387, 773
MspI CCGG 1 cut(s) 693
MspR9I CCNGG 7 cut(s) 66, 189, 300, 362, 415, 521, 693
Mva1269I GAATGC 2 cut(s) 49, 490
MvaI CCWGG 6 cut(s) 66, 189, 300, 362, 415, 521
MwoI GCNNNNNNNGC 7 cut(s) 389, 419, 466, 506, 710, 779, 902
NciI CCSGG 1 cut(s) 693
NcoI CCATGG 1 cut(s) 866
NdeII GATC 3 cut(s) 216, 555, 695
NlaIII CATG 5 cut(s) 624, 685, 844, 870, 1001
NlaIV GGNNCC 6 cut(s) 61, 382, 412, 451, 537, 571
NmuCI GTSAC 1 cut(s) 598
NspI RCATGY 1 cut(s) 624
PasI CCCWGGG 1 cut(s) 361
PciSI GCTCTTC 2 cut(s) 168, 903
PctI GAATGC 2 cut(s) 49, 490
PfeI GAWTC 1 cut(s) 948
PflMI CCANNNNNTGG 1 cut(s) 367
PfoI TCCNGGA 1 cut(s) 691
PkrI GCNGC 7 cut(s) 49, 85, 389, 619, 756, 772, 985
PleI GAGTC 2 cut(s) 100, 865
PpsI GAGTC 2 cut(s) 100, 865
Psp124BI GAGCTC 2 cut(s) 36, 174
Psp1406I AACGTT 1 cut(s) 115
Psp6I CCWGG 6 cut(s) 64, 187, 298, 360, 413, 519
PspGI CCWGG 6 cut(s) 64, 187, 298, 360, 413, 519
PspN4I GGNNCC 6 cut(s) 61, 382, 412, 451, 537, 571
PspPI GGNCC 3 cut(s) 449, 569, 918
PsuI RGATCY 2 cut(s) 555, 695
PvuII CAGCTG 2 cut(s) 251, 773
RsaI GTAC 7 cut(s) 203, 340, 398, 537, 612, 737, 1016
RsaNI GTAC 7 cut(s) 202, 339, 397, 536, 611, 736, 1015
SacI GAGCTC 2 cut(s) 36, 174
SapI GCTCTTC 2 cut(s) 168, 903
SaqAI TTAA 3 cut(s) 39, 333, 834
SatI GCNGC 7 cut(s) 48, 84, 388, 618, 755, 771, 984
Sau3AI GATC 3 cut(s) 216, 555, 695
Sau96I GGNCC 3 cut(s) 449, 569, 918
ScaI AGTACT 2 cut(s) 398, 1016
SchI GAGTC 2 cut(s) 101, 865
ScrFI CCNGG 7 cut(s) 66, 189, 300, 362, 415, 521, 693
SduI GDGCHC 5 cut(s) 36, 163, 174, 385, 898
SfaNI GCATC 2 cut(s) 135, 952
SinI GGWCC 2 cut(s) 449, 569
SmlI CTYRAG 2 cut(s) 440, 851
SmoI CTYRAG 2 cut(s) 440, 851
SsiI CCGC 2 cut(s) 385, 768
SspMI CTAG 1 cut(s) 80
SstI GAGCTC 2 cut(s) 36, 174
StyD4I CCNGG 7 cut(s) 64, 187, 298, 360, 413, 519, 691
StyI CCWWGG 4 cut(s) 376, 866, 921, 942
TaaI ACNGT 1 cut(s) 742
TaiI ACGT 2 cut(s) 118, 651
TatI WGTACW 4 cut(s) 396, 610, 735, 1014
TfiI GAWTC 1 cut(s) 948
Tru1I TTAA 3 cut(s) 39, 333, 834
Tru9I TTAA 3 cut(s) 39, 333, 834
TscAI CASTG 2 cut(s) 103, 187
TseFI GTSAC 1 cut(s) 598
TseI GCWGC 7 cut(s) 47, 83, 387, 617, 754, 770, 983
Tsp45I GTSAC 1 cut(s) 598
TspDTI ATGAA 2 cut(s) 857, 986
TspGWI ACGGA 1 cut(s) 734
TspRI CASTG 2 cut(s) 103, 187
Van91I CCANNNNNTGG 1 cut(s) 367
VpaK11BI GGWCC 2 cut(s) 449, 569
XagI CCTNNNNNAGG 1 cut(s) 224
XceI RCATGY 1 cut(s) 624
XcmI CCANNNNNNNNNTGG 2 cut(s) 206, 368
XspI CTAG 1 cut(s) 80
ZrmI AGTACT 2 cut(s) 398, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.