Prupe.7G116600_v2.0.a1

fructose-bisphosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001206
Physical Location & Seq
Reverse (-)
14164351 .. 14167758
3408 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G116600.1

Sequence Viewer

Length: 1077 bp
ATGTCGGCCTTCAAGGGAAAGTACCATGATGAGCTCATTGCTAATGCTACCTACATTGGCACCCCTGGAAAGGGTATCCTTGCTGCTGATGAGTCAACTGGCACAATTGGCAAGCGCCTTTCAAGCATTAATGTTGAGAATAGTGAGACGAACAGACGCGCCCTCCGTGAGTTACTCTTCACCACCCCTGGTGCCCTCCAATACCTCAGTGGAGTGATTCTCTTTGAGGAAACCCTCTACCAAAAGACAGCTGCTGGTAAGCCATTTGTTGACGTTTTAAGGGAAGGCGGTGTTCTCCCTGGCATCAAGGTTGACAAGGGCACTGTTGAGCTTGCTGGTACCAATGGTGAGACCACAACCCAGGGTCTGGATGGCCTTGGAGAGCGTTGCAAAAAGTACTATGAAGCTGGTGCTCGGTTTGCAAAATGGCGTGCTGTGCTCAATATTGGCCCCAGTGAGCCATCTCAGCTCTCAATCAATGAGAATGCCAATGGGTTGGCCCGATATGCTATCATCTGCCAGGAGAATGGCCTGGTCCCCATTGTTGAGCCAGAGATCCTGGTTGATGGATCCCATGACATTCACAAGTGTGCTGAGGTGACCGAACGTGTTCTTGCTGCATGCTACAAAGCTCTTAACGACCACCATGTTCTGCTTGAAGGAACTCTCTTGAAGCCTAACATGGTGACTCCGGGATCTGATTCACCAAAGGTTGCACCAGAGGTGATTGCTGAGCACACAGTCCGTTCCTTGCAGCGCACCATGCCTGTGGCTGTTCCTGCCGTGGTTTTCTTGTCCGGTGGGCAGAGTGAGGAGGAGGCTACCCTCAACCTCAATGCCATGAACAAATTGAAGGGGAAGAAACCATGGACTCTATCCTTCTCTTTCGGACGGGCTCTTCAGCAAAGCACCCTCAAGGCTTGGGCAGGAAAGGAGGAAAATATTAAGGCGGCACAGGAGGCTTTCCTCACCAGGGCAAAGGCCAACTCGGAAGCGACATTGGGAACCTACAAGGGTGATGCCAAGCTTGGTGAGGGTGCTGCAGAGAGCCTTCATGTCAAGGACTATAAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00680 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00030 map00051 map00680 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
Glycolytic

Protein Analysis

359

Amino Acids

38.56

Weight (kDa)

6.68

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 338
AccB1I GGYRCC 3 cut(s) 59, 191, 338
AccB7I CCANNNNNTGG 1 cut(s) 367
AccII CGCG 1 cut(s) 159
AciI CCGC 2 cut(s) 288, 950
AclWI GGATC 4 cut(s) 550, 564, 577, 703
AcuI CTGAAG 1 cut(s) 884
AfaI GTAC 3 cut(s) 23, 340, 398
AfiI CCNNNNNNNGG 4 cut(s) 70, 71, 367, 973
AflIII ACRYGT 1 cut(s) 607
AgsI TTSAA 5 cut(s) 13, 123, 659, 673, 853
AjnI CCWGG 8 cut(s) 64, 187, 298, 360, 519, 531, 558, 971
AleI CACNNNNGTG 1 cut(s) 588
AluBI AGCT 7 cut(s) 34, 251, 331, 407, 469, 632, 1027
AluI AGCT 7 cut(s) 34, 251, 331, 407, 469, 632, 1027
Alw21I GWGCWC 4 cut(s) 36, 415, 441, 738
Alw26I GTCTC 2 cut(s) 140, 344
AlwI GGATC 4 cut(s) 550, 564, 577, 703
AlwNI CAGNNNCTG 2 cut(s) 254, 367
AoxI GGCC 6 cut(s) 6, 373, 448, 498, 529, 981
ApeKI GCWGC 5 cut(s) 83, 251, 617, 754, 1040
AseI ATTAAT 1 cut(s) 129
Asp700I GAANNNNTTC 1 cut(s) 609
Asp718I GGTACC 1 cut(s) 338
AspLEI GCGC 3 cut(s) 117, 161, 759
AspS9I GGNCC 3 cut(s) 449, 499, 535
AsuC2I CCSGG 1 cut(s) 693
AsuHPI GGTGA 9 cut(s) 172, 359, 610, 696, 697, 736, 961, 1028, 1043
AvaII GGWCC 1 cut(s) 535
BaeGI GKGCMC 2 cut(s) 196, 323
BamHI GGATCC 1 cut(s) 569
BanI GGYRCC 3 cut(s) 59, 191, 338
BanII GRGCYC 2 cut(s) 36, 898
Bbv12I GWGCWC 4 cut(s) 36, 415, 441, 738
BbvCI CCTCAGC 1 cut(s) 594
BbvI GCAGC 5 cut(s) 70, 238, 604, 766, 1027
BccI CCATC 3 cut(s) 365, 469, 560
BceAI ACGGC 1 cut(s) 767
BciT130I CCWGG 8 cut(s) 66, 189, 300, 362, 521, 533, 560, 973
BciVI GTATCC 1 cut(s) 86
BcnI CCSGG 1 cut(s) 693
BcoDI GTCTC 2 cut(s) 140, 344
BfmI CTRYAG 1 cut(s) 1041
BfoI RGCGCY 1 cut(s) 118
BfuI GTATCC 1 cut(s) 86
BisI GCNGC 6 cut(s) 84, 252, 618, 755, 951, 1041
BlpI GCTNAGC 1 cut(s) 732
BlsI GCNGC 6 cut(s) 85, 253, 619, 756, 952, 1042
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 9 cut(s) 66, 189, 300, 362, 521, 533, 560, 693, 973
Bme18I GGWCC 1 cut(s) 535
BmgT120I GGNCC 3 cut(s) 449, 499, 535
BmiI GGNNCC 7 cut(s) 61, 193, 340, 451, 537, 571, 1006
BmrFI CCNGG 9 cut(s) 66, 189, 300, 362, 521, 533, 560, 693, 973
BmrI ACTGGG 1 cut(s) 447
BmsI GCATC 2 cut(s) 312, 1009
BmuI ACTGGG 1 cut(s) 447
BplI GAGNNNNNCTC 2 cut(s) 204, 236
Bpu10I CCTNAGC 1 cut(s) 594
Bpu1102I GCTNAGC 1 cut(s) 732
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 1 cut(s) 693
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 9 cut(s) 64, 187, 298, 360, 361, 376, 783, 866, 972
BsaWI WCCGGW 1 cut(s) 797
BsaXI ACNNNNNCTCC 2 cut(s) 147, 177
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 71, 367, 973
Bse1I ACTGG 2 cut(s) 103, 453
Bse3DI GCAATG 1 cut(s) 36
BseBI CCWGG 8 cut(s) 66, 189, 300, 362, 521, 533, 560, 973
BseDI CCNNGG 9 cut(s) 64, 187, 298, 360, 361, 376, 783, 866, 972
BseGI GGATG 1 cut(s) 376
BseLI CCNNNNNNNGG 4 cut(s) 70, 71, 367, 973
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 4 cut(s) 220, 479, 585, 723
BseNI ACTGG 2 cut(s) 103, 453
BseRI GAGGAG 2 cut(s) 827, 830
BseSI GKGCMC 2 cut(s) 196, 323
BseXI GCAGC 5 cut(s) 70, 238, 604, 766, 1027
Bsh1236I CGCG 1 cut(s) 159
BshFI GGCC 6 cut(s) 8, 375, 450, 500, 531, 983
BshNI GGYRCC 3 cut(s) 59, 191, 338
BsiHKAI GWGCWC 4 cut(s) 36, 415, 441, 738
BsiSI CCGG 2 cut(s) 692, 798
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 4 cut(s) 70, 71, 367, 973
BsmAI GTCTC 2 cut(s) 140, 344
BsmBI CGTCTC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 521
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 6 cut(s) 8, 375, 450, 500, 531, 983
Bso31I GGTCTC 1 cut(s) 344
Bsp1286I GDGCHC 7 cut(s) 36, 196, 323, 415, 441, 738, 898
Bsp143I GATC 3 cut(s) 555, 569, 695
Bsp1720I GCTNAGC 1 cut(s) 732
Bsp19I CCATGG 1 cut(s) 866
BspACI CCGC 2 cut(s) 288, 950
BspANI GGCC 6 cut(s) 8, 375, 450, 500, 531, 983
BspCNI CTCAG 4 cut(s) 219, 478, 586, 724
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 7 cut(s) 61, 193, 340, 451, 537, 571, 1006
BspMAI CTGCAG 1 cut(s) 1045
BspPI GGATC 4 cut(s) 550, 564, 577, 703
BspQI GCTCTTC 1 cut(s) 903
BspT107I GGYRCC 3 cut(s) 59, 191, 338
BspTNI GGTCTC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 36
BsrI ACTGG 2 cut(s) 103, 453
BssECI CCNNGG 9 cut(s) 64, 187, 298, 360, 361, 376, 783, 866, 972
BssMI GATC 3 cut(s) 555, 569, 695
BssT1I CCWWGG 2 cut(s) 376, 866
Bst2UI CCWGG 8 cut(s) 66, 189, 300, 362, 521, 533, 560, 973
Bst4CI ACNGT 2 cut(s) 325, 742
Bst6I CTCTTC 2 cut(s) 182, 903
BstC8I GCNNGC 4 cut(s) 113, 333, 432, 622
BstDEI CTNAG 4 cut(s) 206, 465, 594, 732
BstDSI CCRYGG 2 cut(s) 783, 866
BstEII GGTNACC 1 cut(s) 598
BstF5I GGATG 1 cut(s) 376
BstFNI CGCG 1 cut(s) 159
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 3 cut(s) 117, 161, 759
BstKTI GATC 3 cut(s) 558, 572, 698
BstMAI GTCTC 2 cut(s) 140, 344
BstMBI GATC 3 cut(s) 555, 569, 695
BstMWI GCNNNNNNNGC 9 cut(s) 108, 123, 419, 436, 466, 506, 763, 779, 959
BstNI CCWGG 8 cut(s) 66, 189, 300, 362, 521, 533, 560, 973
BstNSI RCATGY 1 cut(s) 624
BstPI GGTNACC 1 cut(s) 598
BstSCI CCNGG 9 cut(s) 64, 187, 298, 360, 519, 531, 558, 691, 971
BstSFI CTRYAG 1 cut(s) 1041
BstSLI GKGCMC 2 cut(s) 196, 323
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 5 cut(s) 70, 238, 604, 766, 1027
BstX2I RGATCY 3 cut(s) 555, 569, 695
BstXI CCANNNNNNTGG 3 cut(s) 496, 527, 769
BstYI RGATCY 3 cut(s) 555, 569, 695
BsuI GTATCC 1 cut(s) 86
BsuRI GGCC 6 cut(s) 8, 375, 450, 500, 531, 983
BtgI CCRYGG 2 cut(s) 783, 866
BtsCI GGATG 1 cut(s) 376
BtsIMutI CAGTG 3 cut(s) 214, 321, 460
Cac8I GCNNGC 4 cut(s) 113, 333, 432, 622
CaiI CAGNNNCTG 2 cut(s) 254, 367
CfoI GCGC 3 cut(s) 117, 161, 759
Cfr13I GGNCC 3 cut(s) 449, 499, 535
CseI GACGC 1 cut(s) 165
Csp6I GTAC 3 cut(s) 22, 339, 397
CviAII CATG 9 cut(s) 26, 575, 621, 647, 682, 763, 841, 867, 1055
CviQI GTAC 3 cut(s) 22, 339, 397
DdeI CTNAG 4 cut(s) 206, 465, 594, 732
DpnI GATC 3 cut(s) 557, 571, 697
DpnII GATC 3 cut(s) 555, 569, 695
Eam1104I CTCTTC 2 cut(s) 182, 903
EarI CTCTTC 2 cut(s) 182, 903
Ecl136II GAGCTC 1 cut(s) 34
Eco130I CCWWGG 2 cut(s) 376, 866
Eco24I GRGCYC 2 cut(s) 36, 898
Eco31I GGTCTC 1 cut(s) 344
Eco47I GGWCC 1 cut(s) 535
Eco53kI GAGCTC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 884
Eco91I GGTNACC 1 cut(s) 598
EcoICRI GAGCTC 1 cut(s) 34
EcoO65I GGTNACC 1 cut(s) 598
EcoRII CCWGG 8 cut(s) 64, 187, 298, 360, 519, 531, 558, 971
EcoT14I CCWWGG 2 cut(s) 376, 866
EcoT38I GRGCYC 2 cut(s) 36, 898
ErhI CCWWGG 2 cut(s) 376, 866
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 9 cut(s) 29, 578, 624, 650, 685, 766, 844, 870, 1058
FaqI GGGAC 1 cut(s) 521
FatI CATG 9 cut(s) 25, 574, 620, 646, 681, 762, 840, 866, 1054
Fnu4HI GCNGC 6 cut(s) 84, 252, 618, 755, 951, 1041
FokI GGATG 1 cut(s) 383
FriOI GRGCYC 2 cut(s) 36, 898
Fsp4HI GCNGC 6 cut(s) 84, 252, 618, 755, 951, 1041
GlaI GCGC 3 cut(s) 116, 160, 758
GluI GCNGC 6 cut(s) 84, 252, 618, 755, 951, 1041
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 6 cut(s) 8, 375, 450, 500, 531, 983
HapII CCGG 2 cut(s) 692, 798
HgaI GACGC 1 cut(s) 165
HhaI GCGC 3 cut(s) 117, 161, 759
Hin1II CATG 9 cut(s) 29, 578, 624, 650, 685, 766, 844, 870, 1058
Hin6I GCGC 3 cut(s) 115, 159, 757
HinP1I GCGC 3 cut(s) 115, 159, 757
HincII GTYRAC 3 cut(s) 96, 271, 313
HindII GTYRAC 3 cut(s) 96, 271, 313
HindIII AAGCTT 1 cut(s) 1025
HinfI GANTC 5 cut(s) 92, 217, 688, 701, 871
HpaII CCGG 2 cut(s) 692, 798
HphI GGTGA 9 cut(s) 172, 359, 610, 696, 697, 736, 961, 1028, 1043
Hpy166II GTNNAC 3 cut(s) 96, 271, 313
Hpy188I TCNGA 3 cut(s) 700, 890, 991
Hpy188III TCNNGA 2 cut(s) 368, 670
Hpy8I GTNNAC 3 cut(s) 96, 271, 313
HpyAV CCTTC 6 cut(s) 19, 278, 653, 847, 889, 1061
HpyCH4III ACNGT 2 cut(s) 325, 742
HpyCH4IV ACGT 2 cut(s) 273, 607
HpyCH4V TGCA 6 cut(s) 390, 422, 620, 716, 754, 1043
HpyF10VI GCNNNNNNNGC 9 cut(s) 108, 123, 419, 436, 466, 506, 763, 779, 959
HpyF3I CTNAG 4 cut(s) 206, 465, 594, 732
HpySE526I ACGT 2 cut(s) 273, 607
Hsp92II CATG 9 cut(s) 29, 578, 624, 650, 685, 766, 844, 870, 1058
HspAI GCGC 3 cut(s) 115, 159, 757
KpnI GGTACC 1 cut(s) 342
Kzo9I GATC 3 cut(s) 555, 569, 695
LguI GCTCTTC 1 cut(s) 903
Lsp1109I GCAGC 5 cut(s) 70, 238, 604, 766, 1027
LweI GCATC 2 cut(s) 312, 1009
MaeII ACGT 2 cut(s) 273, 607
MaeIII GTNAC 3 cut(s) 171, 598, 685
MalI GATC 3 cut(s) 557, 571, 697
MboI GATC 3 cut(s) 555, 569, 695
MboII GAAGA 3 cut(s) 169, 871, 890
MfeI CAATTG 1 cut(s) 105
MflI RGATCY 3 cut(s) 555, 569, 695
MhlI GDGCHC 7 cut(s) 36, 196, 323, 415, 441, 738, 898
MluCI AATT 2 cut(s) 105, 848
MlyI GAGTC 3 cut(s) 101, 682, 865
MroXI GAANNNNTTC 1 cut(s) 609
MseI TTAA 4 cut(s) 129, 278, 636, 945
MslI CAYNNNNRTG 2 cut(s) 588, 767
MspA1I CMGCKG 1 cut(s) 251
MspI CCGG 2 cut(s) 692, 798
MspR9I CCNGG 9 cut(s) 66, 189, 300, 362, 521, 533, 560, 693, 973
MunI CAATTG 1 cut(s) 105
Mva1269I GAATGC 1 cut(s) 490
MvaI CCWGG 8 cut(s) 66, 189, 300, 362, 521, 533, 560, 973
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 9 cut(s) 108, 123, 419, 436, 466, 506, 763, 779, 959
NciI CCSGG 1 cut(s) 693
NcoI CCATGG 1 cut(s) 866
NdeII GATC 3 cut(s) 555, 569, 695
NlaIII CATG 9 cut(s) 29, 578, 624, 650, 685, 766, 844, 870, 1058
NlaIV GGNNCC 7 cut(s) 61, 193, 340, 451, 537, 571, 1006
NmuCI GTSAC 2 cut(s) 598, 685
NspI RCATGY 1 cut(s) 624
OliI CACNNNNGTG 1 cut(s) 588
PaeI GCATGC 1 cut(s) 624
PasI CCCWGGG 1 cut(s) 361
PciSI GCTCTTC 1 cut(s) 903
PcsI WCGNNNNNNNCGW 1 cut(s) 163
PctI GAATGC 1 cut(s) 490
PdmI GAANNNNTTC 1 cut(s) 609
PfeI GAWTC 2 cut(s) 217, 701
PflMI CCANNNNNTGG 1 cut(s) 367
PfoI TCCNGGA 1 cut(s) 691
PkrI GCNGC 6 cut(s) 85, 253, 619, 756, 952, 1042
PleI GAGTC 3 cut(s) 100, 682, 865
PpsI GAGTC 3 cut(s) 100, 682, 865
PshBI ATTAAT 1 cut(s) 129
Psp124BI GAGCTC 1 cut(s) 36
Psp6I CCWGG 8 cut(s) 64, 187, 298, 360, 519, 531, 558, 971
PspEI GGTNACC 1 cut(s) 598
PspGI CCWGG 8 cut(s) 64, 187, 298, 360, 519, 531, 558, 971
PspN4I GGNNCC 7 cut(s) 61, 193, 340, 451, 537, 571, 1006
PspPI GGNCC 3 cut(s) 449, 499, 535
PstI CTGCAG 1 cut(s) 1045
PstNI CAGNNNCTG 2 cut(s) 254, 367
PsuI RGATCY 3 cut(s) 555, 569, 695
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 3 cut(s) 23, 340, 398
RsaNI GTAC 3 cut(s) 22, 339, 397
RseI CAYNNNNRTG 2 cut(s) 588, 767
SacI GAGCTC 1 cut(s) 36
SapI GCTCTTC 1 cut(s) 903
SaqAI TTAA 4 cut(s) 129, 278, 636, 945
SatI GCNGC 6 cut(s) 84, 252, 618, 755, 951, 1041
Sau3AI GATC 3 cut(s) 555, 569, 695
Sau96I GGNCC 3 cut(s) 449, 499, 535
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 3 cut(s) 101, 682, 865
ScrFI CCNGG 9 cut(s) 66, 189, 300, 362, 521, 533, 560, 693, 973
SduI GDGCHC 7 cut(s) 36, 196, 323, 415, 441, 738, 898
SfaNI GCATC 2 cut(s) 312, 1009
SfcI CTRYAG 1 cut(s) 1041
SinI GGWCC 1 cut(s) 535
SmiMI CAYNNNNRTG 2 cut(s) 588, 767
SmlI CTYRAG 1 cut(s) 914
SmoI CTYRAG 1 cut(s) 914
SphI GCATGC 1 cut(s) 624
Sse9I AATT 2 cut(s) 105, 848
SsiI CCGC 2 cut(s) 288, 950
SspI AATATT 2 cut(s) 445, 943
SstI GAGCTC 1 cut(s) 36
StyD4I CCNGG 9 cut(s) 64, 187, 298, 360, 519, 531, 558, 691, 971
StyI CCWWGG 2 cut(s) 376, 866
TaaI ACNGT 2 cut(s) 325, 742
TaiI ACGT 2 cut(s) 276, 610
TaqII GACCGA 1 cut(s) 617
TasI AATT 2 cut(s) 105, 848
TatI WGTACW 1 cut(s) 396
TauI GCSGC 1 cut(s) 953
TfiI GAWTC 2 cut(s) 217, 701
Tru1I TTAA 4 cut(s) 129, 278, 636, 945
Tru9I TTAA 4 cut(s) 129, 278, 636, 945
TscAI CASTG 3 cut(s) 214, 328, 460
TseFI GTSAC 2 cut(s) 598, 685
TseI GCWGC 5 cut(s) 83, 251, 617, 754, 1040
Tsp45I GTSAC 2 cut(s) 598, 685
TspDTI ATGAA 3 cut(s) 417, 857, 1043
TspGWI ACGGA 2 cut(s) 155, 734
TspRI CASTG 3 cut(s) 214, 328, 460
Van91I CCANNNNNTGG 1 cut(s) 367
VpaK11BI GGWCC 1 cut(s) 535
VspI ATTAAT 1 cut(s) 129
XceI RCATGY 1 cut(s) 624
XcmI CCANNNNNNNNNTGG 2 cut(s) 206, 368
XmnI GAANNNNTTC 1 cut(s) 609
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.