MD12G1018700.v1.1

fructose-bisphosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001206
Physical Location & Seq
Forward (+)
1792377 .. 1795377
3001 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1018700.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGTCGGCCTTCAAGGGAAAGTATCACGATGAGCTCATTGCTAATGCTGCCTACATTGGTACCCCGGGAAAGGGTATCCTTGCTGCCGATGAGTCAACTGGCACGATTGGCAAGCGTCTTTCCAGCATTGGCGTTGAGAATACTGAGACAAACAGGCGCGCTCTCCGTGAGCTGCTTTTCACCACTCCCGGTGCCCTCCAATACCTCAGTGGAGTCATCCTCTTTGAGGAAACCCTCTACCAGAAGACAGCTGCTGGTAAGCCGTTCGTTGATGTTTTGAAGGAGGGCGGTGTCCTTGCTGGAATTAAGGTTGACAAGGGCACTGTTGAGCTTGCTGGTACCAATGGTGAAACCACAACTCAGGGTCTGGATGGCCTTGGCGAACGTTGCAAGAAGTACTACGAAGCTGGTGCCCGATTTGCAAAATGGCGTGCTGTGCTCAAGATTGGCCCCACCGAGCCATCTCAGCTGGCAATCAACGAGAACGCCAATGGGTTGGCCCGATATGCTATCATCTGCCAGGAGAATGGCCTTGTCCCCATTGTTGAGCCAGAGATCCTTGTTGATGGATCCCACGACATTCAGAAGTGTGCTGTGACCGAACGTGTTCTTGCCGCATGCTACAAGGCACTTAACGACCACCATGTCCTGCTTGAAGGGACTCTCTTGAAGCCTAACATGGTGACTCCAGGATCTGATTCCAAAAAGGTTGCGCCCGAGGTGGTTGCCGAGCACACTGTCCGCGCCTTGCAGCGCACCATGCCTGCAGCTGTCCCTGCTGTGGCTTTCCTCTCTGGTGGGCAGAGCGAGGAGGACGCTACCCTCAACCTCAACGCCATGAACAAGTTAAAGGCGAAGAAGCCATGGACTCTTTCATTCTCCTTCGGACGTGCCCTTCAGCAGAGCACTCTCAAGGCTTGGTCAGGAAAGGAGGAGAATGTGAAGAAGGCTCAGGAAGCTTTCCTCACCAGGGCCAAGGCTAACTCGGAAGCGACATTGGGAGCCTACAAGGGTGACGCCAAGCTTGGCGAGGGTGCCGCCGAGAACCTTCATGTCAAGGACTACAAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00680 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00030 map00051 map00680 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
Glycolytic

Protein Analysis

358

Amino Acids

38.31

Weight (kDa)

8.46

Isoelectric Point (pI)

26.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycolytic PF00274 11 - 357 1.5e-172 Fructose-bisphosphate aldolase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 644
Acc65I GGTACC 2 cut(s) 59, 338
AccB1I GGYRCC 5 cut(s) 59, 191, 338, 410, 1034
AccII CGCG 2 cut(s) 159, 744
AciI CCGC 4 cut(s) 288, 615, 742, 1038
AclI AACGTT 1 cut(s) 385
AclWI GGATC 4 cut(s) 550, 564, 577, 700
AcuI CTGAAG 1 cut(s) 881
AcyI GRCGYC 1 cut(s) 1017
AfaI GTAC 3 cut(s) 61, 340, 398
AfiI CCNNNNNNNGG 5 cut(s) 70, 71, 226, 781, 970
AflIII ACRYGT 1 cut(s) 604
AgsI TTSAA 4 cut(s) 13, 280, 656, 670
AjiI CACGTC 1 cut(s) 890
AjnI CCWGG 3 cut(s) 519, 688, 968
AluBI AGCT 9 cut(s) 34, 172, 251, 331, 407, 469, 770, 959, 1024
AluI AGCT 9 cut(s) 34, 172, 251, 331, 407, 469, 770, 959, 1024
Alw21I GWGCWC 4 cut(s) 36, 441, 735, 908
Alw26I GTCTC 1 cut(s) 140
AlwI GGATC 4 cut(s) 550, 564, 577, 700
AlwNI CAGNNNCTG 3 cut(s) 254, 367, 695
Ama87I CYCGRG 2 cut(s) 64, 716
AoxI GGCC 6 cut(s) 6, 373, 448, 498, 529, 972
ApeKI GCWGC 6 cut(s) 47, 83, 172, 251, 751, 767
Asp700I GAANNNNTTC 1 cut(s) 606
Asp718I GGTACC 2 cut(s) 59, 338
AspLEI GCGC 5 cut(s) 159, 161, 715, 746, 756
AspS9I GGNCC 3 cut(s) 449, 499, 972
AsuC2I CCSGG 3 cut(s) 65, 66, 189
AsuHPI GGTGA 5 cut(s) 172, 359, 694, 958, 1025
AvaI CYCGRG 2 cut(s) 64, 716
BaeGI GKGCMC 4 cut(s) 196, 323, 415, 895
BaeI ACNNNNGTAYC 2 cut(s) 43, 76
BamHI GGATCC 1 cut(s) 569
BanI GGYRCC 5 cut(s) 59, 191, 338, 410, 1034
BanII GRGCYC 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 251
Bbv12I GWGCWC 4 cut(s) 36, 441, 735, 908
BbvI GCAGC 6 cut(s) 34, 70, 159, 238, 763, 779
BccI CCATC 3 cut(s) 365, 469, 560
BceAI ACGGC 1 cut(s) 247
BcgI CGANNNNNNTGC 4 cut(s) 392, 426, 707, 741
BciT130I CCWGG 3 cut(s) 521, 690, 970
BciVI GTATCC 1 cut(s) 86
BcnI CCSGG 3 cut(s) 65, 66, 189
BcoDI GTCTC 1 cut(s) 140
BfmI CTRYAG 1 cut(s) 765
BfuI GTATCC 1 cut(s) 86
BisI GCNGC 8 cut(s) 48, 84, 173, 252, 615, 752, 768, 1038
BlsI GCNGC 8 cut(s) 49, 85, 174, 253, 616, 753, 769, 1039
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 6 cut(s) 65, 66, 189, 521, 690, 970
BmeT110I CYCGRG 2 cut(s) 64, 716
BmgBI CACGTC 1 cut(s) 890
BmgT120I GGNCC 3 cut(s) 449, 499, 972
BmiI GGNNCC 8 cut(s) 61, 193, 340, 412, 451, 571, 1003, 1036
BmrFI CCNGG 6 cut(s) 65, 66, 189, 521, 690, 970
BpiI GAAGAC 1 cut(s) 251
BplI GAGNNNNNCTC 2 cut(s) 204, 236
BpmI CTGGAG 1 cut(s) 672
Bpu10I CCTNAGC 1 cut(s) 951
BpuEI CTTGAG 2 cut(s) 425, 896
BpuMI CCSGG 3 cut(s) 65, 66, 189
BsaHI GRCGYC 1 cut(s) 1017
BsaJI CCNNGG 7 cut(s) 63, 64, 376, 717, 863, 969, 975
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 71, 226, 781, 970
Bse1I ACTGG 1 cut(s) 103
Bse3DI GCAATG 1 cut(s) 36
BseBI CCWGG 3 cut(s) 521, 690, 970
BseDI CCNNGG 7 cut(s) 63, 64, 376, 717, 863, 969, 975
BseGI GGATG 2 cut(s) 216, 376
BseLI CCNNNNNNNGG 5 cut(s) 70, 71, 226, 781, 970
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 5 cut(s) 135, 220, 374, 479, 965
BseNI ACTGG 1 cut(s) 103
BsePI GCGCGC 1 cut(s) 157
BseRI GAGGAG 2 cut(s) 824, 947
BseSI GKGCMC 4 cut(s) 196, 323, 415, 895
BseXI GCAGC 6 cut(s) 34, 70, 159, 238, 763, 779
Bsh1236I CGCG 2 cut(s) 159, 744
BshFI GGCC 6 cut(s) 8, 375, 450, 500, 531, 974
BshNI GGYRCC 5 cut(s) 59, 191, 338, 410, 1034
BsiHKAI GWGCWC 4 cut(s) 36, 441, 735, 908
BsiHKCI CYCGRG 2 cut(s) 64, 716
BsiSI CCGG 2 cut(s) 65, 189
BslFI GGGAC 3 cut(s) 521, 673, 758
BslI CCNNNNNNNGG 5 cut(s) 70, 71, 226, 781, 970
BsmAI GTCTC 1 cut(s) 140
BsmFI GGGAC 3 cut(s) 521, 673, 758
BsnI GGCC 6 cut(s) 8, 375, 450, 500, 531, 974
BsoBI CYCGRG 2 cut(s) 64, 716
Bsp1286I GDGCHC 8 cut(s) 36, 196, 323, 415, 441, 735, 895, 908
Bsp143I GATC 3 cut(s) 555, 569, 692
Bsp19I CCATGG 1 cut(s) 863
BspACI CCGC 4 cut(s) 288, 615, 742, 1038
BspANI GGCC 6 cut(s) 8, 375, 450, 500, 531, 974
BspCNI CTCAG 5 cut(s) 136, 219, 373, 478, 964
BspFNI CGCG 2 cut(s) 159, 744
BspLI GGNNCC 8 cut(s) 61, 193, 340, 412, 451, 571, 1003, 1036
BspMAI CTGCAG 1 cut(s) 769
BspPI GGATC 4 cut(s) 550, 564, 577, 700
BspT107I GGYRCC 5 cut(s) 59, 191, 338, 410, 1034
BsrDI GCAATG 1 cut(s) 36
BsrI ACTGG 1 cut(s) 103
BssECI CCNNGG 7 cut(s) 63, 64, 376, 717, 863, 969, 975
BssHII GCGCGC 1 cut(s) 157
BssMI GATC 3 cut(s) 555, 569, 692
BssNI GRCGYC 1 cut(s) 1017
BssT1I CCWWGG 3 cut(s) 376, 863, 975
Bst2UI CCWGG 3 cut(s) 521, 690, 970
Bst4CI ACNGT 2 cut(s) 325, 739
BstACI GRCGYC 1 cut(s) 1017
BstC8I GCNNGC 7 cut(s) 113, 159, 333, 432, 471, 619, 765
BstDEI CTNAG 5 cut(s) 144, 206, 360, 465, 951
BstDSI CCRYGG 1 cut(s) 863
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 2 cut(s) 216, 376
BstFNI CGCG 2 cut(s) 159, 744
BstHHI GCGC 5 cut(s) 159, 161, 715, 746, 756
BstKTI GATC 3 cut(s) 558, 572, 695
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 3 cut(s) 555, 569, 692
BstNI CCWGG 3 cut(s) 521, 690, 970
BstNSI RCATGY 1 cut(s) 621
BstSCI CCNGG 6 cut(s) 63, 64, 187, 519, 688, 968
BstSFI CTRYAG 1 cut(s) 765
BstSLI GKGCMC 4 cut(s) 196, 323, 415, 895
BstUI CGCG 2 cut(s) 159, 744
BstV1I GCAGC 6 cut(s) 34, 70, 159, 238, 763, 779
BstV2I GAAGAC 1 cut(s) 251
BstX2I RGATCY 3 cut(s) 555, 569, 692
BstXI CCANNNNNNTGG 2 cut(s) 496, 527
BstYI RGATCY 3 cut(s) 555, 569, 692
BsuI GTATCC 1 cut(s) 86
BsuRI GGCC 6 cut(s) 8, 375, 450, 500, 531, 974
BtgI CCRYGG 1 cut(s) 863
BtrI CACGTC 1 cut(s) 890
BtsCI GGATG 2 cut(s) 216, 376
BtsIMutI CAGTG 3 cut(s) 214, 321, 735
Cac8I GCNNGC 7 cut(s) 113, 159, 333, 432, 471, 619, 765
CaiI CAGNNNCTG 3 cut(s) 254, 367, 695
CfoI GCGC 5 cut(s) 159, 161, 715, 746, 756
Cfr13I GGNCC 3 cut(s) 449, 499, 972
Cfr9I CCCGGG 1 cut(s) 64
CseI GACGC 3 cut(s) 104, 824, 1025
Csp6I GTAC 3 cut(s) 60, 339, 397
CviAII CATG 7 cut(s) 618, 644, 679, 760, 838, 864, 1052
CviQI GTAC 3 cut(s) 60, 339, 397
DdeI CTNAG 5 cut(s) 144, 206, 360, 465, 951
DpnI GATC 3 cut(s) 557, 571, 694
DpnII GATC 3 cut(s) 555, 569, 692
DrdI GACNNNNNNGTC 1 cut(s) 644
DseDI GACNNNNNNGTC 1 cut(s) 644
Ecl136II GAGCTC 1 cut(s) 34
Eco130I CCWWGG 3 cut(s) 376, 863, 975
Eco24I GRGCYC 1 cut(s) 36
Eco53kI GAGCTC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 881
Eco88I CYCGRG 2 cut(s) 64, 716
EcoICRI GAGCTC 1 cut(s) 34
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoRII CCWGG 3 cut(s) 519, 688, 968
EcoT14I CCWWGG 3 cut(s) 376, 863, 975
EcoT38I GRGCYC 1 cut(s) 36
ErhI CCWWGG 3 cut(s) 376, 863, 975
FaeI CATG 7 cut(s) 621, 647, 682, 763, 841, 867, 1055
FaiI YATR 8 cut(s) 507, 619, 645, 680, 761, 839, 865, 1053
FaqI GGGAC 3 cut(s) 521, 673, 758
FatI CATG 7 cut(s) 617, 643, 678, 759, 837, 863, 1051
Fnu4HI GCNGC 8 cut(s) 48, 84, 173, 252, 615, 752, 768, 1038
FokI GGATG 2 cut(s) 203, 383
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 8 cut(s) 48, 84, 173, 252, 615, 752, 768, 1038
GlaI GCGC 5 cut(s) 158, 160, 714, 745, 755
GluI GCNGC 8 cut(s) 48, 84, 173, 252, 615, 752, 768, 1038
GsuI CTGGAG 1 cut(s) 672
HaeIII GGCC 6 cut(s) 8, 375, 450, 500, 531, 974
HapII CCGG 2 cut(s) 65, 189
HgaI GACGC 3 cut(s) 104, 824, 1025
HhaI GCGC 5 cut(s) 159, 161, 715, 746, 756
Hin1I GRCGYC 1 cut(s) 1017
Hin1II CATG 7 cut(s) 621, 647, 682, 763, 841, 867, 1055
Hin6I GCGC 5 cut(s) 157, 159, 713, 744, 754
HinP1I GCGC 5 cut(s) 157, 159, 713, 744, 754
HincII GTYRAC 2 cut(s) 96, 313
HindII GTYRAC 2 cut(s) 96, 313
HindIII AAGCTT 2 cut(s) 957, 1022
HinfI GANTC 6 cut(s) 92, 213, 661, 685, 698, 868
HpaII CCGG 2 cut(s) 65, 189
HphI GGTGA 5 cut(s) 172, 359, 694, 958, 1025
Hpy166II GTNNAC 2 cut(s) 96, 313
Hpy188I TCNGA 4 cut(s) 585, 697, 887, 988
Hpy188III TCNNGA 6 cut(s) 26, 368, 442, 667, 924, 953
Hpy8I GTNNAC 2 cut(s) 96, 313
HpyAV CCTTC 7 cut(s) 19, 274, 650, 892, 905, 940, 1058
HpyCH4III ACNGT 2 cut(s) 325, 739
HpyCH4IV ACGT 3 cut(s) 385, 604, 889
HpyCH4V TGCA 4 cut(s) 390, 422, 751, 767
HpyF3I CTNAG 5 cut(s) 144, 206, 360, 465, 951
HpySE526I ACGT 3 cut(s) 385, 604, 889
Hsp92I GRCGYC 1 cut(s) 1017
Hsp92II CATG 7 cut(s) 621, 647, 682, 763, 841, 867, 1055
HspAI GCGC 5 cut(s) 157, 159, 713, 744, 754
KpnI GGTACC 2 cut(s) 63, 342
Kzo9I GATC 3 cut(s) 555, 569, 692
LmnI GCTCC 1 cut(s) 1001
Lsp1109I GCAGC 6 cut(s) 34, 70, 159, 238, 763, 779
MaeII ACGT 3 cut(s) 385, 604, 889
MaeIII GTNAC 3 cut(s) 595, 682, 1013
MalI GATC 3 cut(s) 557, 571, 694
MboI GATC 3 cut(s) 555, 569, 692
MboII GAAGA 3 cut(s) 256, 868, 955
MflI RGATCY 3 cut(s) 555, 569, 692
MhlI GDGCHC 8 cut(s) 36, 196, 323, 415, 441, 735, 895, 908
MluCI AATT 1 cut(s) 303
MlyI GAGTC 5 cut(s) 101, 222, 655, 679, 862
MroXI GAANNNNTTC 1 cut(s) 606
MseI TTAA 3 cut(s) 306, 633, 848
MspA1I CMGCKG 3 cut(s) 251, 469, 770
MspI CCGG 2 cut(s) 65, 189
MspR9I CCNGG 6 cut(s) 65, 66, 189, 521, 690, 970
MvaI CCWGG 3 cut(s) 521, 690, 970
MvnI CGCG 2 cut(s) 159, 744
NciI CCSGG 3 cut(s) 65, 66, 189
NcoI CCATGG 1 cut(s) 863
NdeII GATC 3 cut(s) 555, 569, 692
NlaIII CATG 7 cut(s) 621, 647, 682, 763, 841, 867, 1055
NlaIV GGNNCC 8 cut(s) 61, 193, 340, 412, 451, 571, 1003, 1036
NmeAIII GCCGAG 2 cut(s) 754, 1066
NmuCI GTSAC 3 cut(s) 595, 682, 1013
NspI RCATGY 1 cut(s) 621
PaeI GCATGC 1 cut(s) 621
PauI GCGCGC 1 cut(s) 157
PdmI GAANNNNTTC 1 cut(s) 606
PfeI GAWTC 1 cut(s) 698
PfoI TCCNGGA 1 cut(s) 688
PkrI GCNGC 8 cut(s) 49, 85, 174, 253, 616, 753, 769, 1039
PleI GAGTC 5 cut(s) 100, 221, 655, 679, 862
PpsI GAGTC 5 cut(s) 100, 221, 655, 679, 862
Psp124BI GAGCTC 1 cut(s) 36
Psp1406I AACGTT 1 cut(s) 385
Psp6I CCWGG 3 cut(s) 519, 688, 968
PspGI CCWGG 3 cut(s) 519, 688, 968
PspN4I GGNNCC 8 cut(s) 61, 193, 340, 412, 451, 571, 1003, 1036
PspPI GGNCC 3 cut(s) 449, 499, 972
PstI CTGCAG 1 cut(s) 769
PstNI CAGNNNCTG 3 cut(s) 254, 367, 695
PsuI RGATCY 3 cut(s) 555, 569, 692
PteI GCGCGC 1 cut(s) 157
PvuII CAGCTG 3 cut(s) 251, 469, 770
RsaI GTAC 3 cut(s) 61, 340, 398
RsaNI GTAC 3 cut(s) 60, 339, 397
SacI GAGCTC 1 cut(s) 36
SaqAI TTAA 3 cut(s) 306, 633, 848
SatI GCNGC 8 cut(s) 48, 84, 173, 252, 615, 752, 768, 1038
Sau3AI GATC 3 cut(s) 555, 569, 692
Sau96I GGNCC 3 cut(s) 449, 499, 972
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 5 cut(s) 101, 222, 655, 679, 862
ScrFI CCNGG 6 cut(s) 65, 66, 189, 521, 690, 970
SduI GDGCHC 8 cut(s) 36, 196, 323, 415, 441, 735, 895, 908
SfcI CTRYAG 1 cut(s) 765
SmaI CCCGGG 1 cut(s) 66
SmlI CTYRAG 2 cut(s) 440, 911
SmoI CTYRAG 2 cut(s) 440, 911
SphI GCATGC 1 cut(s) 621
Sse9I AATT 1 cut(s) 303
SsiI CCGC 4 cut(s) 288, 615, 742, 1038
SstI GAGCTC 1 cut(s) 36
StyD4I CCNGG 6 cut(s) 63, 64, 187, 519, 688, 968
StyI CCWWGG 3 cut(s) 376, 863, 975
TaaI ACNGT 2 cut(s) 325, 739
TaiI ACGT 3 cut(s) 388, 607, 892
TaqII GACCGA 1 cut(s) 614
TasI AATT 1 cut(s) 303
TatI WGTACW 1 cut(s) 396
TauI GCSGC 2 cut(s) 617, 1040
TfiI GAWTC 1 cut(s) 698
Tru1I TTAA 3 cut(s) 306, 633, 848
Tru9I TTAA 3 cut(s) 306, 633, 848
TscAI CASTG 3 cut(s) 214, 328, 742
TseFI GTSAC 3 cut(s) 595, 682, 1013
TseI GCWGC 6 cut(s) 47, 83, 172, 251, 751, 767
Tsp45I GTSAC 3 cut(s) 595, 682, 1013
TspDTI ATGAA 3 cut(s) 854, 864, 1040
TspGWI ACGGA 1 cut(s) 155
TspMI CCCGGG 1 cut(s) 64
TspRI CASTG 3 cut(s) 214, 328, 742
XagI CCTNNNNNAGG 1 cut(s) 224
XceI RCATGY 1 cut(s) 621
XcmI CCANNNNNNNNNTGG 1 cut(s) 206
XmaI CCCGGG 1 cut(s) 64
XmnI GAANNNNTTC 1 cut(s) 606
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.