MD17G1158400.v1.1

fructose-bisphosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001206
Physical Location & Seq
Forward (+)
15331914 .. 15333020
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1158400.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGTCTGCTTACAGAGGCAAATACGCTGATGAGCTTATCGCCAATGCTGCCTACATCGGGACCCCTGGAAAGGGTATTCTTGCTGCTGATGAGTCAAATGGCATAATCGGCAAACGTTTTGCTAGCATCAATGTTGAGAATGTTCAATCAAATAGACGTGCTCTTCGCGAACTCCTTTTCACTGCCCCTGATGTGCTCCAATACCTTAATGGAGTTATCCTCTTTGAGGAAACCCTCTACCAGAAGACCACTTCAGGCAAGCCATTCGTTGATGTCTTGAAGGAAAGAGGTGTTCTCCCTAGAATTAAGGTCGACCAGGGTACCGTTGAGCTCGCCGGAACCAATGGTGAGACCATAACCCAGGGTCTTGATGGCCTTGCCCAGTGTTGCCAGAAGTACTATGAAGTTGGTGCACGGTTCGTAAAGTGGCGTGCTGTGCTTAAGATTAGCCCTATTGAACCATCTCAGCTGTCCATCCAAGAGAATGCCAACGGGTTAGCTCATTATGCCATCATCTGCCAGGAGAATGGCCTAGTCCCCATTGTTGAGCCAGAGATCCTGGTTAATGGACCACATTCCATCAATAAGTGTGCAGATATGACCGAATGTGTTCTTATCGCATGCTATAAGGCATTTAGCGACCACTATGTCCTGCTTGAAGGGACTCTCTTGAAACCCAACATGGTTACCCTAAGATTTGATGCCAAGAAGGCTTCACCAGAAGTGATTGCTGAGTACACAGTTCGTGCCTTGTTGCGCACTACCTATGTAGCTGTCTCTGCTGTTGTGTTCTTATCCGGTGGACAGAGTAAGGATGAGGCTACCCTCAACCTTAACGCAATGAACCAACTCAAAGGAAATAAGCCGTGGTTTCTATCCTTCTCCTTTAGACGTGCTCTTCAGCATAGCACACTGAAGGCATGGGCAGGGAAAGAGGAAAACATCCCAAAGGCACATGTTGCTCTCCTCACAAGGGCCAAGGCCAACTCAGAAGCTACTTTTGGAATCTACAAGGGTGATGCCAAACTCGGTGAGGGACAAGGATGGGGAGGAGCTGGAAAAGAAGGATCTCTGGGAAGGTACGTTTCGGATTTCGGATTTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00680 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00030 map00051 map00680 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
Glycolytic

Protein Analysis

369

Amino Acids

40.1

Weight (kDa)

8.57

Isoelectric Point (pI)

29.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycolytic PF00274 11 - 342 1.4e-148 Fructose-bisphosphate aldolase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 647
Acc16I TGCGCA 1 cut(s) 758
Acc65I GGTACC 1 cut(s) 320
AccB1I GGYRCC 1 cut(s) 320
AccI GTMKAC 1 cut(s) 312
AccII CGCG 1 cut(s) 168
AclI AACGTT 1 cut(s) 115
AclWI GGATC 2 cut(s) 550, 1075
AcuI CTGAAG 3 cut(s) 237, 884, 935
AfaI GTAC 4 cut(s) 322, 398, 737, 1082
AfiI CCNNNNNNNGG 5 cut(s) 57, 70, 71, 226, 973
AflII CTTAAG 1 cut(s) 440
AflIII ACRYGT 1 cut(s) 955
AgsI TTSAA 5 cut(s) 146, 280, 458, 659, 673
AjiI CACGTC 2 cut(s) 158, 893
AjnI CCWGG 5 cut(s) 64, 315, 360, 519, 558
AjuI GAANNNNNNNTTGG 2 cut(s) 984, 1016
AluBI AGCT 7 cut(s) 34, 331, 469, 500, 773, 995, 1055
AluI AGCT 7 cut(s) 34, 331, 469, 500, 773, 995, 1055
Alw21I GWGCWC 5 cut(s) 163, 198, 333, 415, 898
Alw26I GTCTC 2 cut(s) 344, 781
Alw44I GTGCAC 1 cut(s) 411
AlwI GGATC 2 cut(s) 550, 1075
AoxI GGCC 4 cut(s) 373, 529, 975, 981
ApaLI GTGCAC 1 cut(s) 411
ApeKI GCWGC 2 cut(s) 47, 83
ArsI GACNNNNNNTTYG 2 cut(s) 147, 179
Asp700I GAANNNNTTC 1 cut(s) 609
Asp718I GGTACC 1 cut(s) 320
AspLEI GCGC 1 cut(s) 759
AspS9I GGNCC 3 cut(s) 60, 569, 975
AsuHPI GGTGA 4 cut(s) 359, 708, 1028, 1043
AsuNHI GCTAGC 1 cut(s) 122
AvaII GGWCC 2 cut(s) 60, 569
BaeGI GKGCMC 1 cut(s) 415
BaeI ACNNNNGTAYC 2 cut(s) 304, 337
BanI GGYRCC 1 cut(s) 320
BanII GRGCYC 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 251
Bbv12I GWGCWC 5 cut(s) 163, 198, 333, 415, 898
BbvI GCAGC 2 cut(s) 34, 70
BccI CCATC 6 cut(s) 365, 469, 482, 518, 587, 1038
BceAI ACGGC 1 cut(s) 850
BcgI CGANNNNNNTGC 2 cut(s) 247, 281
BciT130I CCWGG 5 cut(s) 66, 317, 362, 521, 560
BcoDI GTCTC 2 cut(s) 344, 781
BfaI CTAG 3 cut(s) 123, 300, 533
BfrI CTTAAG 1 cut(s) 440
BglI GCCNNNNNGGC 1 cut(s) 710
BisI GCNGC 2 cut(s) 48, 84
BlsI GCNGC 2 cut(s) 49, 85
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 5 cut(s) 66, 317, 362, 521, 560
Bme18I GGWCC 2 cut(s) 60, 569
BmgBI CACGTC 2 cut(s) 158, 893
BmgT120I GGNCC 3 cut(s) 60, 569, 975
BmiI GGNNCC 4 cut(s) 61, 62, 322, 340
BmrFI CCNGG 5 cut(s) 66, 317, 362, 521, 560
BmrI ACTGGG 1 cut(s) 376
BmsI GCATC 3 cut(s) 135, 691, 1009
BmtI GCTAGC 1 cut(s) 126
BmuI ACTGGG 1 cut(s) 376
BpiI GAAGAC 1 cut(s) 251
BplI GAGNNNNNCTC 4 cut(s) 204, 236, 279, 311
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 6 cut(s) 64, 316, 360, 361, 866, 978
BsaWI WCCGGW 1 cut(s) 797
Bsc4I CCNNNNNNNGG 5 cut(s) 57, 70, 71, 226, 973
Bse1I ACTGG 1 cut(s) 382
Bse3DI GCAATG 1 cut(s) 846
BseBI CCWGG 5 cut(s) 66, 317, 362, 521, 560
BseDI CCNNGG 6 cut(s) 64, 316, 360, 361, 866, 978
BseGI GGATG 4 cut(s) 474, 820, 942, 1049
BseLI CCNNNNNNNGG 5 cut(s) 57, 70, 71, 226, 973
BseMI GCAATG 1 cut(s) 846
BseMII CTCAG 3 cut(s) 479, 723, 1002
BseNI ACTGG 1 cut(s) 382
BseRI GAGGAG 2 cut(s) 956, 1065
BseSI GKGCMC 1 cut(s) 415
BseXI GCAGC 2 cut(s) 34, 70
BsgI GTGCAG 1 cut(s) 612
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 4 cut(s) 375, 531, 977, 983
BshNI GGYRCC 1 cut(s) 320
BsiHKAI GWGCWC 5 cut(s) 163, 198, 333, 415, 898
BsiSI CCGG 2 cut(s) 336, 798
BslFI GGGAC 4 cut(s) 73, 521, 676, 1050
BslI CCNNNNNNNGG 5 cut(s) 57, 70, 71, 226, 973
BsmAI GTCTC 2 cut(s) 344, 781
BsmFI GGGAC 4 cut(s) 73, 521, 676, 1050
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 4 cut(s) 375, 531, 977, 983
Bso31I GGTCTC 1 cut(s) 344
Bsp1286I GDGCHC 5 cut(s) 163, 198, 333, 415, 898
Bsp143I GATC 2 cut(s) 555, 1067
Bsp68I TCGCGA 1 cut(s) 168
BspANI GGCC 4 cut(s) 375, 531, 977, 983
BspCNI CTCAG 3 cut(s) 478, 724, 1001
BspFNI CGCG 1 cut(s) 168
BspLI GGNNCC 4 cut(s) 61, 62, 322, 340
BspOI GCTAGC 1 cut(s) 126
BspPI GGATC 2 cut(s) 550, 1075
BspQI GCTCTTC 2 cut(s) 168, 903
BspT107I GGYRCC 1 cut(s) 320
BspTI CTTAAG 1 cut(s) 440
BspTNI GGTCTC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 846
BsrI ACTGG 1 cut(s) 382
BssECI CCNNGG 6 cut(s) 64, 316, 360, 361, 866, 978
BssMI GATC 2 cut(s) 555, 1067
BssT1I CCWWGG 1 cut(s) 978
Bst2UI CCWGG 5 cut(s) 66, 317, 362, 521, 560
Bst4CI ACNGT 3 cut(s) 325, 417, 742
Bst6I CTCTTC 2 cut(s) 168, 903
BstAFI CTTAAG 1 cut(s) 440
BstAPI GCANNNNNTGC 1 cut(s) 959
BstC8I GCNNGC 5 cut(s) 124, 260, 333, 432, 622
BstDEI CTNAG 4 cut(s) 465, 692, 732, 988
BstDSI CCRYGG 1 cut(s) 866
BstEII GGTNACC 1 cut(s) 685
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 4 cut(s) 474, 820, 942, 1049
BstFNI CGCG 1 cut(s) 168
BstHHI GCGC 1 cut(s) 759
BstKTI GATC 2 cut(s) 558, 1070
BstMAI GTCTC 2 cut(s) 344, 781
BstMBI GATC 2 cut(s) 555, 1067
BstMWI GCNNNNNNNGC 7 cut(s) 47, 108, 436, 506, 710, 779, 959
BstNI CCWGG 5 cut(s) 66, 317, 362, 521, 560
BstNSI RCATGY 2 cut(s) 624, 959
BstPI GGTNACC 1 cut(s) 685
BstSCI CCNGG 5 cut(s) 64, 315, 360, 519, 558
BstSLI GKGCMC 1 cut(s) 415
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 34, 70
BstV2I GAAGAC 1 cut(s) 251
BstX2I RGATCY 2 cut(s) 555, 1067
BstXI CCANNNNNNTGG 1 cut(s) 527
BstYI RGATCY 2 cut(s) 555, 1067
BsuRI GGCC 4 cut(s) 375, 531, 977, 983
BtgI CCRYGG 1 cut(s) 866
BtrI CACGTC 2 cut(s) 158, 893
BtsCI GGATG 4 cut(s) 474, 820, 942, 1049
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 3 cut(s) 180, 389, 911
BtuMI TCGCGA 1 cut(s) 168
Cac8I GCNNGC 5 cut(s) 124, 260, 333, 432, 622
CfoI GCGC 1 cut(s) 759
Cfr13I GGNCC 3 cut(s) 60, 569, 975
Csp6I GTAC 4 cut(s) 321, 397, 736, 1081
CviAII CATG 5 cut(s) 621, 682, 921, 956, 1104
CviQI GTAC 4 cut(s) 321, 397, 736, 1081
DdeI CTNAG 4 cut(s) 465, 692, 732, 988
DpnI GATC 2 cut(s) 557, 1069
DpnII GATC 2 cut(s) 555, 1067
DrdI GACNNNNNNGTC 1 cut(s) 647
DseDI GACNNNNNNGTC 1 cut(s) 647
Eam1104I CTCTTC 2 cut(s) 168, 903
EarI CTCTTC 2 cut(s) 168, 903
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 1 cut(s) 978
Eco24I GRGCYC 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 344
Eco47I GGWCC 2 cut(s) 60, 569
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 3 cut(s) 237, 884, 935
Eco91I GGTNACC 1 cut(s) 685
EcoICRI GAGCTC 1 cut(s) 331
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 60
EcoO65I GGTNACC 1 cut(s) 685
EcoRII CCWGG 5 cut(s) 64, 315, 360, 519, 558
EcoT14I CCWWGG 1 cut(s) 978
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 978
FaeI CATG 5 cut(s) 624, 685, 924, 959, 1107
FaqI GGGAC 4 cut(s) 73, 521, 676, 1050
FatI CATG 5 cut(s) 620, 681, 920, 955, 1103
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 2 cut(s) 48, 84
FokI GGATG 4 cut(s) 461, 827, 929, 1056
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 2 cut(s) 48, 84
FspBI CTAG 3 cut(s) 123, 300, 533
FspI TGCGCA 1 cut(s) 758
GlaI GCGC 1 cut(s) 758
GluI GCNGC 2 cut(s) 48, 84
HaeIII GGCC 4 cut(s) 375, 531, 977, 983
HapII CCGG 2 cut(s) 336, 798
HhaI GCGC 1 cut(s) 759
Hin1II CATG 5 cut(s) 624, 685, 924, 959, 1107
Hin6I GCGC 1 cut(s) 757
HinP1I GCGC 1 cut(s) 757
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 3 cut(s) 92, 664, 1005
HpaII CCGG 2 cut(s) 336, 798
HphI GGTGA 4 cut(s) 359, 708, 1028, 1043
Hpy166II GTNNAC 4 cut(s) 313, 413, 738, 803
Hpy188I TCNGA 3 cut(s) 991, 1090, 1097
Hpy188III TCNNGA 5 cut(s) 58, 167, 277, 368, 670
Hpy8I GTNNAC 4 cut(s) 313, 413, 738, 803
HpyAV CCTTC 7 cut(s) 274, 653, 703, 889, 910, 1058, 1071
HpyCH4III ACNGT 3 cut(s) 325, 417, 742
HpyCH4IV ACGT 4 cut(s) 115, 157, 892, 1083
HpyCH4V TGCA 3 cut(s) 413, 593, 1103
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 108, 436, 506, 710, 779, 959
HpyF3I CTNAG 4 cut(s) 465, 692, 732, 988
HpySE526I ACGT 4 cut(s) 115, 157, 892, 1083
Hsp92II CATG 5 cut(s) 624, 685, 924, 959, 1107
HspAI GCGC 1 cut(s) 757
KflI GGGWCCC 1 cut(s) 60
KpnI GGTACC 1 cut(s) 324
Kzo9I GATC 2 cut(s) 555, 1067
LguI GCTCTTC 2 cut(s) 168, 903
LmnI GCTCC 2 cut(s) 201, 1052
Lsp1109I GCAGC 2 cut(s) 34, 70
LweI GCATC 3 cut(s) 135, 691, 1009
MaeI CTAG 3 cut(s) 123, 300, 533
MaeII ACGT 4 cut(s) 115, 157, 892, 1083
MaeIII GTNAC 1 cut(s) 685
MalI GATC 2 cut(s) 557, 1069
MboI GATC 2 cut(s) 555, 1067
MboII GAAGA 3 cut(s) 155, 256, 890
MflI RGATCY 2 cut(s) 555, 1067
MhlI GDGCHC 5 cut(s) 163, 198, 333, 415, 898
MluCI AATT 1 cut(s) 303
MlyI GAGTC 2 cut(s) 101, 658
MroXI GAANNNNTTC 1 cut(s) 609
MseI TTAA 5 cut(s) 207, 306, 441, 564, 834
MspA1I CMGCKG 1 cut(s) 469
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 2 cut(s) 336, 798
MspR9I CCNGG 5 cut(s) 66, 317, 362, 521, 560
Mva1269I GAATGC 1 cut(s) 490
MvaI CCWGG 5 cut(s) 66, 317, 362, 521, 560
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 7 cut(s) 47, 108, 436, 506, 710, 779, 959
NdeII GATC 2 cut(s) 555, 1067
NheI GCTAGC 1 cut(s) 122
NlaIII CATG 5 cut(s) 624, 685, 924, 959, 1107
NlaIV GGNNCC 4 cut(s) 61, 62, 322, 340
NruI TCGCGA 1 cut(s) 168
NsbI TGCGCA 1 cut(s) 758
NspI RCATGY 2 cut(s) 624, 959
PaeI GCATGC 1 cut(s) 624
PasI CCCWGGG 1 cut(s) 361
PciI ACATGT 1 cut(s) 955
PciSI GCTCTTC 2 cut(s) 168, 903
PctI GAATGC 1 cut(s) 490
PdmI GAANNNNTTC 1 cut(s) 609
PfeI GAWTC 1 cut(s) 1005
PkrI GCNGC 2 cut(s) 49, 85
PleI GAGTC 2 cut(s) 100, 658
PpsI GAGTC 2 cut(s) 100, 658
PpuMI RGGWCCY 1 cut(s) 60
PscI ACATGT 1 cut(s) 955
Psp124BI GAGCTC 1 cut(s) 333
Psp1406I AACGTT 1 cut(s) 115
Psp5II RGGWCCY 1 cut(s) 60
Psp6I CCWGG 5 cut(s) 64, 315, 360, 519, 558
PspEI GGTNACC 1 cut(s) 685
PspGI CCWGG 5 cut(s) 64, 315, 360, 519, 558
PspN4I GGNNCC 4 cut(s) 61, 62, 322, 340
PspPI GGNCC 3 cut(s) 60, 569, 975
PspPPI RGGWCCY 1 cut(s) 60
PsuI RGATCY 2 cut(s) 555, 1067
PvuII CAGCTG 1 cut(s) 469
RruI TCGCGA 1 cut(s) 168
RsaI GTAC 4 cut(s) 322, 398, 737, 1082
RsaNI GTAC 4 cut(s) 321, 397, 736, 1081
SacI GAGCTC 1 cut(s) 333
SalI GTCGAC 1 cut(s) 311
SapI GCTCTTC 2 cut(s) 168, 903
SaqAI TTAA 5 cut(s) 207, 306, 441, 564, 834
SatI GCNGC 2 cut(s) 48, 84
Sau3AI GATC 2 cut(s) 555, 1067
Sau96I GGNCC 3 cut(s) 60, 569, 975
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 2 cut(s) 101, 658
ScrFI CCNGG 5 cut(s) 66, 317, 362, 521, 560
SduI GDGCHC 5 cut(s) 163, 198, 333, 415, 898
SfaNI GCATC 3 cut(s) 135, 691, 1009
SinI GGWCC 2 cut(s) 60, 569
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
SphI GCATGC 1 cut(s) 624
Sse9I AATT 1 cut(s) 303
SspMI CTAG 3 cut(s) 123, 300, 533
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 5 cut(s) 64, 315, 360, 519, 558
StyI CCWWGG 1 cut(s) 978
TaaI ACNGT 3 cut(s) 325, 417, 742
TaiI ACGT 4 cut(s) 118, 160, 895, 1086
TaqI TCGA 1 cut(s) 312
TaqII GACCGA 1 cut(s) 617
TasI AATT 1 cut(s) 303
TatI WGTACW 2 cut(s) 396, 735
TfiI GAWTC 1 cut(s) 1005
Tru1I TTAA 5 cut(s) 207, 306, 441, 564, 834
Tru9I TTAA 5 cut(s) 207, 306, 441, 564, 834
TscAI CASTG 3 cut(s) 187, 389, 918
TseI GCWGC 2 cut(s) 47, 83
TspDTI ATGAA 2 cut(s) 417, 857
TspRI CASTG 3 cut(s) 187, 389, 918
Vha464I CTTAAG 1 cut(s) 440
VneI GTGCAC 1 cut(s) 411
VpaK11BI GGWCC 2 cut(s) 60, 569
XagI CCTNNNNNAGG 1 cut(s) 224
XceI RCATGY 2 cut(s) 624, 959
XcmI CCANNNNNNNNNTGG 2 cut(s) 206, 368
XmiI GTMKAC 1 cut(s) 312
XmnI GAANNNNTTC 1 cut(s) 609
XspI CTAG 3 cut(s) 123, 300, 533
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.