MD12G1018800.v1.1

fructose-bisphosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001206
Physical Location & Seq
Forward (+)
1800195 .. 1802619
2425 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1018800.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGTCTGCTTACAGAGGCAAATACGCTGATGAGCTTATCGCCAATGCTGCCTACATCGGCACCCCTGGAAAGGGTATTCTCGCTGCTGATGAGTCAACTGGCACAATCGGCAAACGTTTTGCCAGCATCAATGTTGAGAATGTTGAATCAAACAGGCGTGCTCTTCGTGAACTGCTCTTCACTGCCCCTGATGTGCTCCAATACCTTAGTGGAGTTATCCTCTTTGAGGAAACCCTCTACCAGAAGACCGCTTCAGGAAAGCCATTCGTTGATGTCTTGAAGGAAGGTGGTGTTCTCCCTGGAATTAAGGTCGACAAGGGTACCGTTGAGCTTGCCGGAACCAATGGTGAGACCACAACCCAGGGTCTTGATGGCCTTGCCCAGCGTTGCCAGAAGTACTATGAAGCTGGTGCACGGTTCGCAAAGTGGCGTGCTGTGCTCAAGATTGGCCCCAATGAACCATCTCAGCTGTCCATCCAAGAGAATGCCAACGGGTTGGCTCGTTATGCCATCATCTGCCAGGAGAATGGCCTAGTGCCCATCGTTGAGCCAGAGATCCTGGTCGATGGACCCCATTCCATCGATAAGTGTGCCGATGTGACTGAACGTGTTCTTGCCGCATGCTACAAGGCCCTTAACGACCACCATGTCCTGCTTGAAGGAACTCTCTTGAAACCCAACATGGTTACCCCAGGATCTGATGCCAAGAAGGTTTCACCAGAAGTGATTGCTAAGTACACAGTTCGCGCCTTGTTGCGCACTACCCCAGCAGCTGTCCCTGCTGTTGTGTTCTTGTCCGGTGGACAGAGTGAGGAGGAGGCTACCCTCAACCTCAACGCCATGAACCAACTCAAGGGAAAGAAGCCGTGGTCTCTATCCTTCTCCTTCGGACGTGCTCTTCAGCAGAGCACACTGAAGGCATGGGCAGGGAAAGAGGGAAACATCCCAAAGGCGCAGGCTGCTCTCCTCACAAGGGCCAAGGCCAACTCAGAAGCTACTCTTGGAATCTACAAGGGTGATGCCAAGCTCGGTGAGGGTGCCGCTGAGTCTCTCCATGTGAAGGATTACAAGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00680 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00030 map00051 map00680 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
Glycolytic

Protein Analysis

359

Amino Acids

38.41

Weight (kDa)

8.46

Isoelectric Point (pI)

24.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycolytic PF00274 11 - 358 2.7e-172 Fructose-bisphosphate aldolase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 647
Acc16I TGCGCA 1 cut(s) 758
Acc65I GGTACC 1 cut(s) 320
AccB1I GGYRCC 3 cut(s) 59, 320, 1037
AccI GTMKAC 1 cut(s) 312
AccII CGCG 1 cut(s) 747
AciI CCGC 3 cut(s) 249, 618, 1041
AclI AACGTT 1 cut(s) 115
AclWI GGATC 2 cut(s) 550, 703
AcuI CTGAAG 3 cut(s) 237, 884, 935
AfaI GTAC 4 cut(s) 322, 398, 737, 1073
AfiI CCNNNNNNNGG 6 cut(s) 70, 71, 226, 853, 973, 1060
AflIII ACRYGT 1 cut(s) 607
AgsI TTSAA 4 cut(s) 146, 280, 659, 673
AjiI CACGTC 1 cut(s) 893
AjnI CCWGG 6 cut(s) 64, 298, 360, 519, 558, 691
AjuI GAANNNNNNNTTGG 2 cut(s) 984, 1016
AluBI AGCT 7 cut(s) 34, 331, 407, 469, 773, 995, 1027
AluI AGCT 7 cut(s) 34, 331, 407, 469, 773, 995, 1027
Alw21I GWGCWC 6 cut(s) 163, 198, 415, 441, 898, 911
Alw26I GTCTC 3 cut(s) 344, 876, 1053
Alw44I GTGCAC 1 cut(s) 411
AlwI GGATC 2 cut(s) 550, 703
AlwNI CAGNNNCTG 2 cut(s) 698, 773
AoxI GGCC 6 cut(s) 373, 448, 529, 630, 975, 981
ApaLI GTGCAC 1 cut(s) 411
ApeKI GCWGC 4 cut(s) 47, 83, 770, 959
Asp700I GAANNNNTTC 1 cut(s) 609
Asp718I GGTACC 1 cut(s) 320
AspLEI GCGC 3 cut(s) 749, 759, 955
AspS9I GGNCC 4 cut(s) 449, 569, 631, 975
AsuHPI GGTGA 4 cut(s) 359, 708, 1028, 1043
AvaII GGWCC 1 cut(s) 569
BaeGI GKGCMC 2 cut(s) 415, 540
BaeI ACNNNNGTAYC 2 cut(s) 304, 337
BanI GGYRCC 3 cut(s) 59, 320, 1037
BbsI GAAGAC 1 cut(s) 251
Bbv12I GWGCWC 6 cut(s) 163, 198, 415, 441, 898, 911
BbvI GCAGC 4 cut(s) 34, 70, 782, 946
BccI CCATC 7 cut(s) 365, 469, 482, 518, 548, 560, 587
BceAI ACGGC 1 cut(s) 850
BcgI CGANNNNNNTGC 2 cut(s) 572, 606
BciT130I CCWGG 6 cut(s) 66, 300, 362, 521, 560, 693
BcoDI GTCTC 3 cut(s) 344, 876, 1053
BfaI CTAG 1 cut(s) 533
BisI GCNGC 6 cut(s) 48, 84, 618, 771, 960, 1041
BlsI GCNGC 6 cut(s) 49, 85, 619, 772, 961, 1042
BmcAI AGTACT 2 cut(s) 398, 1073
Bme1390I CCNGG 6 cut(s) 66, 300, 362, 521, 560, 693
Bme18I GGWCC 1 cut(s) 569
BmgBI CACGTC 1 cut(s) 893
BmgT120I GGNCC 4 cut(s) 449, 569, 631, 975
BmiI GGNNCC 6 cut(s) 61, 322, 340, 451, 571, 1039
BmrFI CCNGG 6 cut(s) 66, 300, 362, 521, 560, 693
BmsI GCATC 3 cut(s) 135, 691, 1009
BpiI GAAGAC 1 cut(s) 251
BplI GAGNNNNNCTC 2 cut(s) 204, 236
BpuEI CTTGAG 2 cut(s) 425, 836
Bsa29I ATCGAT 1 cut(s) 582
BsaI GGTCTC 2 cut(s) 344, 876
BsaJI CCNNGG 7 cut(s) 64, 298, 360, 361, 691, 866, 978
BsaWI WCCGGW 1 cut(s) 797
Bsc4I CCNNNNNNNGG 6 cut(s) 70, 71, 226, 853, 973, 1060
Bse1I ACTGG 1 cut(s) 103
BseBI CCWGG 6 cut(s) 66, 300, 362, 521, 560, 693
BseCI ATCGAT 1 cut(s) 582
BseDI CCNNGG 7 cut(s) 64, 298, 360, 361, 691, 866, 978
BseGI GGATG 2 cut(s) 474, 942
BseLI CCNNNNNNNGG 6 cut(s) 70, 71, 226, 853, 973, 1060
BseMII CTCAG 3 cut(s) 479, 1002, 1035
BseNI ACTGG 1 cut(s) 103
BseRI GAGGAG 3 cut(s) 827, 830, 956
BseSI GKGCMC 2 cut(s) 415, 540
BseXI GCAGC 4 cut(s) 34, 70, 782, 946
BseYI CCCAGC 2 cut(s) 381, 766
Bsh1236I CGCG 1 cut(s) 747
BshFI GGCC 6 cut(s) 375, 450, 531, 632, 977, 983
BshNI GGYRCC 3 cut(s) 59, 320, 1037
BshVI ATCGAT 1 cut(s) 582
BsiHKAI GWGCWC 6 cut(s) 163, 198, 415, 441, 898, 911
BsiSI CCGG 2 cut(s) 336, 798
BslFI GGGAC 1 cut(s) 761
BslI CCNNNNNNNGG 6 cut(s) 70, 71, 226, 853, 973, 1060
BsmAI GTCTC 3 cut(s) 344, 876, 1053
BsmFI GGGAC 1 cut(s) 761
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 6 cut(s) 375, 450, 531, 632, 977, 983
Bso31I GGTCTC 2 cut(s) 344, 876
Bsp1286I GDGCHC 7 cut(s) 163, 198, 415, 441, 540, 898, 911
Bsp143I GATC 2 cut(s) 555, 695
BspACI CCGC 3 cut(s) 249, 618, 1041
BspANI GGCC 6 cut(s) 375, 450, 531, 632, 977, 983
BspCNI CTCAG 3 cut(s) 478, 1001, 1036
BspDI ATCGAT 1 cut(s) 582
BspFNI CGCG 1 cut(s) 747
BspLI GGNNCC 6 cut(s) 61, 322, 340, 451, 571, 1039
BspPI GGATC 2 cut(s) 550, 703
BspQI GCTCTTC 3 cut(s) 168, 182, 903
BspT107I GGYRCC 3 cut(s) 59, 320, 1037
BspTNI GGTCTC 2 cut(s) 344, 876
BsrI ACTGG 1 cut(s) 103
BssECI CCNNGG 7 cut(s) 64, 298, 360, 361, 691, 866, 978
BssMI GATC 2 cut(s) 555, 695
BssT1I CCWWGG 1 cut(s) 978
Bst2UI CCWGG 6 cut(s) 66, 300, 362, 521, 560, 693
Bst4CI ACNGT 3 cut(s) 325, 417, 742
Bst6I CTCTTC 3 cut(s) 168, 182, 903
BstC8I GCNNGC 6 cut(s) 124, 159, 333, 432, 622, 957
BstDEI CTNAG 5 cut(s) 206, 465, 732, 988, 1044
BstDSI CCRYGG 1 cut(s) 866
BstEII GGTNACC 1 cut(s) 685
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 2 cut(s) 474, 942
BstFNI CGCG 1 cut(s) 747
BstHHI GCGC 3 cut(s) 749, 759, 955
BstKTI GATC 2 cut(s) 558, 698
BstMAI GTCTC 3 cut(s) 344, 876, 1053
BstMBI GATC 2 cut(s) 555, 695
BstMWI GCNNNNNNNGC 7 cut(s) 47, 108, 419, 436, 506, 779, 959
BstNI CCWGG 6 cut(s) 66, 300, 362, 521, 560, 693
BstNSI RCATGY 1 cut(s) 624
BstPI GGTNACC 1 cut(s) 685
BstSCI CCNGG 6 cut(s) 64, 298, 360, 519, 558, 691
BstSLI GKGCMC 2 cut(s) 415, 540
BstUI CGCG 1 cut(s) 747
BstV1I GCAGC 4 cut(s) 34, 70, 782, 946
BstV2I GAAGAC 1 cut(s) 251
BstX2I RGATCY 2 cut(s) 555, 695
BstXI CCANNNNNNTGG 2 cut(s) 496, 527
BstYI RGATCY 2 cut(s) 555, 695
Bsu15I ATCGAT 1 cut(s) 582
BsuRI GGCC 6 cut(s) 375, 450, 531, 632, 977, 983
BsuTUI ATCGAT 1 cut(s) 582
BtgI CCRYGG 1 cut(s) 866
BtrI CACGTC 1 cut(s) 893
BtsCI GGATG 2 cut(s) 474, 942
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 2 cut(s) 180, 911
Cac8I GCNNGC 6 cut(s) 124, 159, 333, 432, 622, 957
CaiI CAGNNNCTG 2 cut(s) 698, 773
CfoI GCGC 3 cut(s) 749, 759, 955
Cfr13I GGNCC 4 cut(s) 449, 569, 631, 975
ClaI ATCGAT 1 cut(s) 582
Csp6I GTAC 4 cut(s) 321, 397, 736, 1072
CviAII CATG 6 cut(s) 621, 647, 682, 841, 921, 1055
CviQI GTAC 4 cut(s) 321, 397, 736, 1072
DdeI CTNAG 5 cut(s) 206, 465, 732, 988, 1044
DpnI GATC 2 cut(s) 557, 697
DpnII GATC 2 cut(s) 555, 695
DrdI GACNNNNNNGTC 1 cut(s) 647
DseDI GACNNNNNNGTC 1 cut(s) 647
Eam1104I CTCTTC 3 cut(s) 168, 182, 903
EarI CTCTTC 3 cut(s) 168, 182, 903
Eco130I CCWWGG 1 cut(s) 978
Eco31I GGTCTC 2 cut(s) 344, 876
Eco47I GGWCC 1 cut(s) 569
Eco57I CTGAAG 3 cut(s) 237, 884, 935
Eco91I GGTNACC 1 cut(s) 685
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 631
EcoO65I GGTNACC 1 cut(s) 685
EcoRII CCWGG 6 cut(s) 64, 298, 360, 519, 558, 691
EcoT14I CCWWGG 1 cut(s) 978
ErhI CCWWGG 1 cut(s) 978
FaeI CATG 6 cut(s) 624, 650, 685, 844, 924, 1058
FaiI YATR 8 cut(s) 402, 507, 622, 648, 683, 842, 922, 1056
FalI AAGNNNNNCTT 2 cut(s) 984, 1016
FaqI GGGAC 1 cut(s) 761
FatI CATG 6 cut(s) 620, 646, 681, 840, 920, 1054
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 6 cut(s) 48, 84, 618, 771, 960, 1041
FokI GGATG 2 cut(s) 461, 929
Fsp4HI GCNGC 6 cut(s) 48, 84, 618, 771, 960, 1041
FspBI CTAG 1 cut(s) 533
FspI TGCGCA 1 cut(s) 758
GlaI GCGC 3 cut(s) 748, 758, 954
GluI GCNGC 6 cut(s) 48, 84, 618, 771, 960, 1041
GsaI CCCAGC 2 cut(s) 385, 770
HaeIII GGCC 6 cut(s) 375, 450, 531, 632, 977, 983
HapII CCGG 2 cut(s) 336, 798
HhaI GCGC 3 cut(s) 749, 759, 955
Hin1II CATG 6 cut(s) 624, 650, 685, 844, 924, 1058
Hin6I GCGC 3 cut(s) 747, 757, 953
HinP1I GCGC 3 cut(s) 747, 757, 953
HincII GTYRAC 2 cut(s) 96, 313
HindII GTYRAC 2 cut(s) 96, 313
HinfI GANTC 4 cut(s) 92, 146, 1005, 1046
HpaII CCGG 2 cut(s) 336, 798
HphI GGTGA 4 cut(s) 359, 708, 1028, 1043
Hpy166II GTNNAC 6 cut(s) 96, 170, 313, 413, 738, 803
Hpy188I TCNGA 3 cut(s) 700, 890, 991
Hpy188III TCNNGA 6 cut(s) 167, 255, 277, 368, 442, 670
Hpy8I GTNNAC 6 cut(s) 96, 170, 313, 413, 738, 803
HpyAV CCTTC 8 cut(s) 274, 278, 653, 703, 889, 895, 910, 1054
HpyCH4III ACNGT 3 cut(s) 325, 417, 742
HpyCH4IV ACGT 3 cut(s) 115, 607, 892
HpyCH4V TGCA 1 cut(s) 413
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 108, 419, 436, 506, 779, 959
HpyF3I CTNAG 5 cut(s) 206, 465, 732, 988, 1044
HpySE526I ACGT 3 cut(s) 115, 607, 892
Hsp92II CATG 6 cut(s) 624, 650, 685, 844, 924, 1058
HspAI GCGC 3 cut(s) 747, 757, 953
KpnI GGTACC 1 cut(s) 324
Kzo9I GATC 2 cut(s) 555, 695
LguI GCTCTTC 3 cut(s) 168, 182, 903
LmnI GCTCC 1 cut(s) 201
Lsp1109I GCAGC 4 cut(s) 34, 70, 782, 946
LweI GCATC 3 cut(s) 135, 691, 1009
MaeI CTAG 1 cut(s) 533
MaeII ACGT 3 cut(s) 115, 607, 892
MaeIII GTNAC 2 cut(s) 598, 685
MalI GATC 2 cut(s) 557, 697
MboI GATC 2 cut(s) 555, 695
MboII GAAGA 4 cut(s) 155, 169, 256, 890
MflI RGATCY 2 cut(s) 555, 695
MhlI GDGCHC 7 cut(s) 163, 198, 415, 441, 540, 898, 911
MluCI AATT 1 cut(s) 303
MlyI GAGTC 2 cut(s) 101, 1055
MroXI GAANNNNTTC 1 cut(s) 609
MseI TTAA 2 cut(s) 306, 636
MspA1I CMGCKG 3 cut(s) 469, 773, 1043
MspI CCGG 2 cut(s) 336, 798
MspR9I CCNGG 6 cut(s) 66, 300, 362, 521, 560, 693
Mva1269I GAATGC 1 cut(s) 490
MvaI CCWGG 6 cut(s) 66, 300, 362, 521, 560, 693
MvnI CGCG 1 cut(s) 747
MwoI GCNNNNNNNGC 7 cut(s) 47, 108, 419, 436, 506, 779, 959
NdeII GATC 2 cut(s) 555, 695
NlaIII CATG 6 cut(s) 624, 650, 685, 844, 924, 1058
NlaIV GGNNCC 6 cut(s) 61, 322, 340, 451, 571, 1039
NmuCI GTSAC 1 cut(s) 598
NsbI TGCGCA 1 cut(s) 758
NspI RCATGY 1 cut(s) 624
PaeI GCATGC 1 cut(s) 624
PasI CCCWGGG 1 cut(s) 361
PciSI GCTCTTC 3 cut(s) 168, 182, 903
PctI GAATGC 1 cut(s) 490
PdmI GAANNNNTTC 1 cut(s) 609
PfeI GAWTC 2 cut(s) 146, 1005
PkrI GCNGC 6 cut(s) 49, 85, 619, 772, 961, 1042
PleI GAGTC 2 cut(s) 100, 1054
PpsI GAGTC 2 cut(s) 100, 1054
Psp1406I AACGTT 1 cut(s) 115
Psp6I CCWGG 6 cut(s) 64, 298, 360, 519, 558, 691
PspEI GGTNACC 1 cut(s) 685
PspFI CCCAGC 2 cut(s) 381, 766
PspGI CCWGG 6 cut(s) 64, 298, 360, 519, 558, 691
PspN4I GGNNCC 6 cut(s) 61, 322, 340, 451, 571, 1039
PspPI GGNCC 4 cut(s) 449, 569, 631, 975
PstNI CAGNNNCTG 2 cut(s) 698, 773
PsuI RGATCY 2 cut(s) 555, 695
PvuII CAGCTG 2 cut(s) 469, 773
RsaI GTAC 4 cut(s) 322, 398, 737, 1073
RsaNI GTAC 4 cut(s) 321, 397, 736, 1072
SalI GTCGAC 1 cut(s) 311
SapI GCTCTTC 3 cut(s) 168, 182, 903
SaqAI TTAA 2 cut(s) 306, 636
SatI GCNGC 6 cut(s) 48, 84, 618, 771, 960, 1041
Sau3AI GATC 2 cut(s) 555, 695
Sau96I GGNCC 4 cut(s) 449, 569, 631, 975
ScaI AGTACT 2 cut(s) 398, 1073
SchI GAGTC 2 cut(s) 101, 1055
ScrFI CCNGG 6 cut(s) 66, 300, 362, 521, 560, 693
SduI GDGCHC 7 cut(s) 163, 198, 415, 441, 540, 898, 911
SfaNI GCATC 3 cut(s) 135, 691, 1009
SinI GGWCC 1 cut(s) 569
SmlI CTYRAG 2 cut(s) 440, 851
SmoI CTYRAG 2 cut(s) 440, 851
SphI GCATGC 1 cut(s) 624
Sse9I AATT 1 cut(s) 303
SsiI CCGC 3 cut(s) 249, 618, 1041
SspMI CTAG 1 cut(s) 533
StyD4I CCNGG 6 cut(s) 64, 298, 360, 519, 558, 691
StyI CCWWGG 1 cut(s) 978
TaaI ACNGT 3 cut(s) 325, 417, 742
TaiI ACGT 3 cut(s) 118, 610, 895
TaqI TCGA 3 cut(s) 312, 564, 582
TasI AATT 1 cut(s) 303
TatI WGTACW 3 cut(s) 396, 735, 1071
TauI GCSGC 2 cut(s) 620, 1043
TfiI GAWTC 2 cut(s) 146, 1005
Tru1I TTAA 2 cut(s) 306, 636
Tru9I TTAA 2 cut(s) 306, 636
TscAI CASTG 2 cut(s) 187, 918
TseFI GTSAC 1 cut(s) 598
TseI GCWGC 4 cut(s) 47, 83, 770, 959
Tsp45I GTSAC 1 cut(s) 598
TspDTI ATGAA 3 cut(s) 417, 471, 857
TspRI CASTG 2 cut(s) 187, 918
VneI GTGCAC 1 cut(s) 411
VpaK11BI GGWCC 1 cut(s) 569
XagI CCTNNNNNAGG 1 cut(s) 224
XceI RCATGY 1 cut(s) 624
XcmI CCANNNNNNNNNTGG 2 cut(s) 206, 368
XmiI GTMKAC 1 cut(s) 312
XmnI GAANNNNTTC 1 cut(s) 609
XspI CTAG 1 cut(s) 533
ZrmI AGTACT 2 cut(s) 398, 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.